CN114014428B - Environment-friendly flocculant and application thereof - Google Patents
Environment-friendly flocculant and application thereof Download PDFInfo
- Publication number
- CN114014428B CN114014428B CN202111475645.7A CN202111475645A CN114014428B CN 114014428 B CN114014428 B CN 114014428B CN 202111475645 A CN202111475645 A CN 202111475645A CN 114014428 B CN114014428 B CN 114014428B
- Authority
- CN
- China
- Prior art keywords
- dextran
- solution
- flocculant
- citric acid
- environment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5263—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using natural chemical compounds
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
- C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Hydrology & Water Resources (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- General Chemical & Material Sciences (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
An environment-friendly flocculant and application thereof belong to the technical field of environment-friendly flocculants. The most widely used flocculating agents at present are mainly inorganic flocculating agents (such as polyaluminium chloride) and synthetic organic flocculating agents (such as polyacrylamide), and the flocculating agents have certain toxicity and are difficult to degrade, and secondary pollution is caused by wide use and waste residues cannot be treated. The invention provides an environment-friendly flocculant which is nontoxic and harmless, has no secondary pollution and good performance, and an application thereof, and the main component of the flocculant is dextran-citric acid anionic polymer. The preparation method comprises the following steps: adjusting the pH of the citric acid solution to 7-9 by using sodium hydroxide, uniformly mixing and stirring the citric acid solution and the dextran solution, and adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to react for 12-36 hours, wherein the molar ratio of edc to citric acid is 1-2: 1, a step of; adding ethanol for precipitation, washing and drying the precipitate to obtain the flocculant. The flocculated soybean meal waste liquid can be used for livestock feeding, and has simple preparation and high efficiency.
Description
Technical Field
An environment-friendly flocculant and application thereof belong to the technical field of environment-friendly flocculants.
Background
With the development of industry, more and more industrial wastewater is generated, the environmental pollution problem caused by the industrial wastewater is more and more severe, the flocculation method is the sewage treatment method which is the most widely applied, the flocculation method has the advantages of high efficiency, low cost and the like, the flocculation process mainly plays a role of a flocculant, the most widely applied flocculant at present is mainly an inorganic flocculant (such as polyaluminium chloride) and a synthetic organic flocculant (such as polyacrylamide), although the flocculant has high flocculation activity and low cost, the flocculant has certain toxicity and is difficult to degrade, secondary pollution is caused by wide application, new environmental and human health problems are caused, and solid residues obtained after flocculation and precipitation are not reasonable treatment methods.
The technology of fermentation, washing and the like in the bean product industry can generate a large amount of high-turbidity and turbid wastewater, which contains a large amount of oxidizable organic matters and cannot be directly discharged, but means that the wastewater contains abundant nutrients such as protein, amino acid, vitamin, inorganic salt and the like, so that the wastewater has huge recycling value, and in the existing wastewater treatment method in the bean product industry, a large amount of heavy metals or toxic monomers are introduced by adopting a flocculation treatment means, so that the wastewater is difficult to treat cleanly, a large amount of nutrient wastes are caused, and an environment-friendly flocculant which is nontoxic and harmless, degradable, free of secondary pollution and huge in recycling value is urgently needed at present.
Disclosure of Invention
The technical problems to be solved by the invention are as follows: overcomes the defects of the prior art, and provides an environment-friendly flocculant which is nontoxic and harmless, degradable, free from secondary pollution and good in performance and application thereof.
The technical scheme adopted for solving the technical problems is as follows: an environment-friendly flocculant is characterized in that: the main component is dextran-citric acid anionic polymer.
The high molecular dextran and citric acid natural products are applied to the field of flocculants, the dextran provides extremely high molecular weight, the citric acid provides enough grafting anions, the citric acid and the dextran generate higher coordination effect, the complementary synergy is easy to graft, and the final dextran-citric acid anionic flocculant contains a large number of anionic active groups and can be used as a high-performance biological high molecular flocculant for removing impurity particles with cationic groups in water. Does not contain metal ions and toxic and harmful organic monomers, has good biocompatibility and biodegradability and has wider application range.
The preparation method of the environment-friendly flocculant comprises the following steps: adjusting the pH of the citric acid solution to 7-9 by using strong alkali, mixing and stirring the citric acid solution and the dextran solution uniformly, adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to react for 12-36 hours, wherein the molar ratio of the 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to the citric acid is 1-2: 1, a step of; adding ethanol for precipitation, washing and drying the precipitate to obtain the flocculant.
The preferable mixing time of the dextran solution and the citric acid solution is 3-5 min, and the reaction temperature after adding the 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride is preferably room temperature; the strong base is common alkali which does not introduce heavy metal ions, and is further preferably sodium hydroxide.
The reaction mechanism of the above reaction is shown in the following formula (1):
the method comprises the steps of preparing high molecular dextran through catalyzing sucrose reaction with dextrorotase, and then using 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) as an activating reagent, and connecting citric acid to a dextran molecular chain through esterification reaction between the high molecular dextran and the citric acid to obtain the anionic dextran flocculant.
The invention innovatively applies two natural products of high molecular dextran and citric acid to the field of flocculants, and utilizes the esterification reaction of the high molecular dextran and the citric acid to carry out derivatization of the dextran, so that the obtained dextran-citric acid anionic flocculant has extremely high molecular weight, the citric acid provides enough grafted anions, the citric acid and the dextran generate higher coordination effect, the grafting is easy, the complementary synergy is easy, and the final dextran-citric acid anionic flocculant contains a large number of anionic active groups and can be used as a biopolymer flocculant with good performance for removing impurity particles with cationic groups in water. The preparation process and the product are nontoxic and harmless, and degradable, the raw material sources are wide, the derivatization method is mild and safe, the operation process steps are simple, the product quality is controllable, and the prepared flocculant does not contain metal ions and toxic and harmful organic monomers, has good biocompatibility and biodegradability, and does not bring harm to human health and environmental safety. Meanwhile, the flocculation performance is high, the dosage is small, the flocculation treatment device is applicable to pH 4-9, the precipitation is more thorough, the flocculation treatment device can be used with chitosan and other cationic flocculants to treat waste water in the hematite and bean product industries, and the flocculation effect is remarkably improved.
Preferably, the concentration of the dextran solution is 10-60 g/L.
The preferred concentration can be fully reacted with citric acid solution and higher concentration of dextran-citric acid anionic flocculant is obtained, which is easy for final precipitation and purification.
Preferably, the dextran in the dextran solution is obtained from a sucrose solution by an enzymatic reaction of dextran sucrase.
The dextran sucrase is a hydrolysis synthase for synthesizing the dextran by taking sucrose as a substrate for catalytic synthesis, and is commonly produced by leuconostoc mesenteroides, can also be produced by genetic engineering bacteria capable of highly expressing the dextran sucrase as described in Chinese patent CN101363009A, and can also be produced by mixed fermentation as described in Chinese patent CN 105132390A.
The dextran obtained by adopting the preferred dextran preparation method has higher activity and higher controllability, so that the dextran with higher molecular weight can be catalyzed and synthesized under the condition suitable for process control, so that the dextran-citric acid anionic flocculant with higher flocculation efficiency and smaller dosage is obtained, and the method is more suitable for the preparation method of the environment-friendly flocculant.
Further preferably, the enzyme catalytic reaction comprises preparing a sucrose solution from acetic acid-calcium acetate buffer solution with pH of 5.0-5.5, adding dextrorotatory sucrase into the sucrose solution for enzyme catalytic reaction, reacting at 20-28 ℃ until dynamic viscosity reaches 8000-10000 CPS, terminating the reaction, precipitating high molecular weight dextran with ethanol, and washing to obtain dextran.
And (3) obtaining a high molecular dextran solution if the obtained dextran is dissolved in water, and finally obtaining dextran solid powder if the high molecular dextran solution is placed in a constant-temperature blast oven at 30-50 ℃ for drying and grinding. In the preparation process of the environment-friendly flocculant, the dextran is easier to participate in the reaction in a solution mode, so that the reaction efficiency is improved.
Further preferably, the molar concentration of the sucrose solution is 0.55-0.75 mol/L, and the concentration of the dextrorotatory sucrase is 5.0-6.0U/mL.
Preferably, after the mixture is uniformly mixed and stirred, the mole ratio of citric acid to dextran anhydroglucose units in the solution is 0.1-1.0: 1.
an application of an environment-friendly flocculant is characterized in that: the environment-friendly flocculant is added into the soybean meal wastewater in a stirring state, stirred for 0.5-2 min at a rotating speed of 150-200 rpm, stirred for 0.5-2 min at a rotating speed of 50-80 rpm, and filtered.
The environment-friendly flocculant contains a large number of anionic active groups, is suitable for removing impurity particles with cationic groups in the soybean meal wastewater, and flocculates and settles the impurity particles such as protein, amino acid and the like in the soybean meal wastewater through the flocculation mechanism effects such as charge neutralization, adsorption bridging and the like, thereby playing a good clarification role, and similarly being applicable to the fields such as other foods, medicines and the like; in addition, by utilizing the characteristics of no toxicity, no harm and green environmental protection of the invention, the flocculated solid sediment can be used in the fields of livestock feeding and the like, thus the application value is regained, the double treatment of wastewater and solid waste is realized, the economic benefit is obtained, and the environment is protected.
Further preferably, the stirring speed of the environment-friendly flocculant in the adding process is 150-200 rpm.
The whole adding process is divided into a mode of firstly stirring rapidly and then stirring slowly, the preferable rotating speed is more suitable for fully combining and reacting anions and cations in the initial flocculation stage, and in the later flocculation stage, the flocculation structure is protected, and full sedimentation is ensured, so that a better flocculation effect is achieved.
Preferably, the environment-friendly flocculant is prepared into a solution of 1-10 g/L and then added into the soybean meal wastewater.
The environment-friendly flocculant is prepared into the mother solution and then participates in flocculation reaction, so that the dispersion effect of the flocculant can be effectively improved, impurities in the waste liquid can be ensured to fully contact with the anionic dextran-based flocculant solution, and the flocculation effect is improved.
Preferably, the final concentration of the environment-friendly flocculant in the soybean meal wastewater is 200-400 mg/L.
The preferable flocculant dosage can fully settle most of protein, amino acid and other impurities in the soybean meal wastewater, and can effectively avoid introducing excessive anionic groups, ensure flocculation effect, save flocculant dosage and reduce the impurities in the treated waste liquid.
Compared with the prior art, the invention has the following beneficial effects: according to the invention, citric acid is grafted onto a high molecular dextran chain through esterification reaction, so that the natural anionic dextran flocculant is successfully prepared, raw materials used in the preparation are all derived from natural products, and the method has the advantages of environmental protection, no toxicity, no harm, wide sources and the like, the adopted derivatization method is mild and safe, the operation process steps are simple, the product quality is controllable, the prepared flocculant does not contain metal ions and toxic and harmful organic monomers, and has good biocompatibility and biodegradability, and no harm to human health and environmental safety is caused. Compared with the inorganic and synthetic organic flocculants sold in the market at present, the dextran-citric acid anionic flocculant is safe and environment-friendly, has no secondary pollution, does not have the problems of metal residue, corrosion pipeline, toxic monomer residue and the like, has wider application range, and can be used in clarification processes in the fields of food, medicine and the like.
Drawings
FIG. 1 is a graph showing the results of a flocculation experiment for a simulated waste water sample of the hematite suspension of example 1.
Detailed Description
The present invention will be further described with reference to the following examples, with example 1 being the best mode of carrying out the invention.
Example 1
The preparation method of the dextran-citric acid anionic environment-friendly flocculant comprises the following steps of:
1) Preparing a sucrose solution with the concentration of 5.0U/mL by adopting an acetic acid-calcium acetate buffer solution with the pH value of=5.4, and adding a dextran sucrase solution into the sucrose solution with the concentration of 5.58 mol/L; enzyme catalytic reaction is carried out for 24 hours at 25 ℃ in a shaking table at 100 rpm; the dynamic viscosity reaches 9000CPS at the end of the reaction; precipitating high molecular weight dextran product with ethanol, washing for 2 times, and drying in a 40 deg.C constant temperature blast oven; grinding after the high molecular weight dextran product is dried to obtain high molecular weight dextran solid powder;
2) Preparing a high molecular weight dextran solution and a citric acid solution, regulating the pH value of the citric acid solution to 7.5 by using a sodium hydroxide solution, mixing and stirring the citric acid solution and the dextran solution for 5min, wherein the concentration of the dextran in the mixed solution is 25g/L, and the molar ratio of the citric acid to the dextran anhydroglucose units is 0.5:1, a step of; EDC was added in a molar ratio of EDC to citric acid of 1:1, a step of; the reaction solution is placed at room temperature for reaction for 24 hours, absolute ethanol is added to precipitate a product, and the product is washed with 80% ethanol for 2 times; and (3) drying and grinding the product in a blast oven with constant temperature of 40 ℃ to obtain white dry solid powder, namely the anionic dextran flocculant.
Example 2
On the basis of the embodiment 1, the concentration of the dextran in the mixed solution in the step 2) is set to be 50g/L, and the molar ratio of the final citric acid to the dextran anhydroglucose units is 1:1, the other conditions were the same as in example 1.
Example 3
On the basis of the embodiment 1, the concentration of the dextran in the mixed solution in the step 2) is set to be 10g/L, and the molar ratio of the final citric acid to the dextran anhydroglucose units is 0.1:1, the other conditions were the same as in example 1.
Example 4
On the basis of the example 1, EDC is added in the step 2) to make the mole ratio of EDC to citric acid be 2:1, a step of; other conditions were the same as in example 1.
Example 5
The application of the dextran-citric acid anionic environment-friendly flocculant is that the anionic dextran-based flocculant obtained in the example 1 is used for clarifying soybean meal wastewater and recycling filter residues. The method comprises the following steps:
preparing a 1g/L flocculant solution of the dextran-citric acid anionic environment-friendly flocculant prepared in the example 1, adding the flocculant solution into the soybean meal wastewater at a rotating speed of 200rpm until the concentration of the dextran-citric acid anionic environment-friendly flocculant in the soybean meal wastewater is 300mg/L, continuing stirring for 1min, and stirring for 1min after the rotating speed is reduced to 80 rpm; stopping stirring, standing for sedimentation, filtering, and finishing the treatment.
After the waste water is clarified by the method, the turbidity of the waste water is greatly reduced, the water body becomes clear, the original suspended particles form floccules in a lump shape through flocculation, the floccules are settled under the action of gravity, and after the floccules are filtered and washed, heavy metal or thiocyanate monomers are detected, so that the waste water can be used for livestock feeding.
Example 6
On the basis of the example 5, the concentration of the dextran-citric acid anionic environment-friendly flocculant added to the soybean meal wastewater is 200mg/L, and other conditions are the same as the example 5.
After the waste water is clarified by the method, the turbidity of the waste water is greatly reduced, the water body becomes clear, the original suspended particles form floccules in a lump shape through flocculation, the floccules are settled under the action of gravity, and after the floccules are filtered and washed, heavy metal or thiocyanate monomers are detected, so that the waste water can be used for livestock feeding.
Comparative example 1
A flocculant, the protein recovery rate of commercial flocculant polymeric ferric sulfate to bean pulp waste water can reach more than 90%, but polymeric ferric sulfate as inorganic flocculant can pollute the recovered protein, the recovered protein can not be used as feed, the resource utilization of waste can not be realized, and the flocculated waste liquid contains a large amount of metal ion residues, which is easy to cause secondary pollution.
Comparative example 2
The preparation method of the dextran-citric acid anionic environment-friendly flocculant comprises the following steps of:
preparing a high molecular weight dextran solution and a citric acid solution by using the high molecular weight dextran solid powder obtained in the example 1, mixing and stirring the citric acid solution and the dextran solution for 5min, wherein the concentration of the dextran in the mixed solution is 25g/L, and the molar ratio of the citric acid to the dextran anhydroglucose units is 1:1, a step of; adding sodium hypophosphite (PHP) with the mass ratio of the sodium hypophosphite to the citric acid of 1:10; placing the reaction solution in a water bath kettle at 60 ℃ for reaction for 5 hours, adding absolute ethyl alcohol to precipitate a product after the reaction is finished, and washing the product with 80% ethyl alcohol for 2 times; and (3) drying and grinding the product in a blast oven with constant temperature of 40 ℃ to obtain white dry solid powder, namely the anionic dextran flocculant.
Comparative example 3
The preparation method of the dextran-citric acid anionic environment-friendly flocculant comprises the following steps of:
using the high molecular weight dextran solid powder obtained in example 1, a high molecular weight dextran solution and a citric acid solution were prepared, the pH of the citric acid solution was adjusted to 4 with a sodium hydroxide solution, and the mixture was stirred for 5 minutes with a concentration of dextran of 25g/L in the mixed solution, and a molar ratio of citric acid to dextran anhydroglucose units of 0.5:1, a step of; EDC was added in a molar ratio of EDC to citric acid of 1:1, a step of; the reaction solution is placed at room temperature for reaction for 24 hours, absolute ethanol is added to precipitate a product, and the product is washed with 80% ethanol for 2 times; and (3) drying and grinding the product in a blast oven with constant temperature of 40 ℃ to obtain white dry solid powder, namely the anionic dextran flocculant.
Comparative example 4
The preparation method of the dextran-citric acid anionic environment-friendly flocculant comprises the following steps of:
preparing a high molecular weight dextran solid powder obtained in example 1, namely preparing a high molecular weight dextran solution and a malic acid solution, regulating the pH value of the malic acid solution to 7 by using a sodium hydroxide solution, mixing and stirring the malic acid solution and the dextran solution for 5min, wherein the concentration of the dextran in the mixed solution is 25g/L, and the molar ratio of malic acid to dextran anhydroglucose units is 1:1, a step of; EDC is added, and the molar ratio of EDC to malic acid is 1:1, a step of; the reaction solution is placed at room temperature for reaction for 24 hours, absolute ethanol is added to precipitate a product, and the product is washed with 80% ethanol for 2 times; and (3) drying and grinding the product in a blast oven with constant temperature of 40 ℃ to obtain white dry solid powder, namely the anionic dextran flocculant.
Performance testing
0.25g of hematite powder is weighed and stirred in 250mL of distilled water for 5min, and a simulated waste water sample of the hematite suspension with the concentration of 1.0g/L is obtained.
Dissolving the dextran-citric acid anion type environment-friendly flocculant powder prepared in examples 1-4 and comparative examples 1 and 2 in distilled water to prepare a dextran-citric acid anion type environment-friendly flocculant mother solution with the concentration of 1mg/L, adding 2ml of the prepared dextran-citric acid anion type flocculant mother solution into the hematite suspension, stirring while adding, standing for 2min after stirring, taking a supernatant, detecting an absorbance value at 550nm, and calculating flocculation efficiency F% according to the following formula:
F%=（F0-F1）/ F0 * 100%
wherein F0 and F1 are absorbance values at 550nm of the hematite suspension before and after flocculation, respectively.
Under the condition that the pH value of the hematite suspension is 6, the final flocculation efficiency F% of the examples 1-4 is 84.4%, 83.5%, 82.7% and 82.5% respectively; the final flocculation efficiencies F% of comparative examples 1 to 4 were 90.1%, 70.5%, 61.1% and 60.9%, respectively.
The dextran-citric acid anionic environment-friendly flocculant obtained in the embodiment 1 is additionally tested under the condition of pH 4-9, under different system pH values, the use amount of the dextran-citric acid anionic flocculant is increased from 0mg/L to 72mg/L along with the change trend of flocculation efficiency, the experimental result is shown in the attached figure 1, the result shows that under the condition of pH 4-9, under different system pH values, the use amount of the dextran-citric acid anionic flocculant is increased from 0mg/L to 72mg/L along with the change trend of flocculation efficiency is similar, the use amount of the dextran-citric acid anionic flocculant is increased rapidly, the higher flocculation efficiency is achieved when the use amount is only 8mg/L, then the rising rate is slowed down, and the flocculation efficiency is gradually decreased after the highest value is reached. At pH 6, the optimum amount is the lowest, and the corresponding flocculation efficiency is the highest, 24mg/L and 84.4%, respectively. At pH4, the highest flocculation efficiency is lower but still reaches 70%, and the dextran-citric acid anionic flocculant has excellent flocculation activity in a wider pH range.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the invention in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to the equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims (7)
1. An environment-friendly flocculant is characterized in that: the components comprise dextran-citric acid anionic polymer; the preparation method comprises the following steps: the pH value of the citric acid solution is regulated to 7-9 by strong alkali, the citric acid solution and the dextran solution are mixed and stirred uniformly, and the molar ratio of citric acid to dextran anhydroglucose units in the solution is 0.1-1.0: 1, a step of; adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to react for 12-36 hours, wherein the molar ratio of the 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to the citric acid is 1-2: 1, a step of; adding ethanol for precipitation, washing and drying the precipitation to obtain a flocculant; the concentration of the dextran solution is 10-60 g/L.
2. The environmentally friendly flocculant of claim 1, wherein: the dextran in the dextran solution is obtained from a sucrose solution by an enzyme catalytic reaction of dextran sucrase.
3. The environmentally friendly flocculant of claim 2, wherein: the enzyme catalytic reaction comprises the steps of preparing a sucrose solution from acetic acid-calcium acetate buffer solution with pH of 5.0-5.5, adding dextrorotatory sucrase into the sucrose solution for enzyme catalytic reaction, reacting at 20-28 ℃ until dynamic viscosity reaches 8000-10000 CPS, terminating the reaction, precipitating high molecular weight dextran with ethanol, and washing to obtain dextran.
4. An environmentally friendly flocculant according to claim 3, wherein: the molar concentration of the sucrose solution is 0.55-0.75 mol/L, and the dextran sucrase adopts a solution with the concentration of 5.0-6.0U/mL.
5. An application of an environment-friendly flocculant is characterized in that: adding the environment-friendly flocculant according to any one of claims 1-4 into soybean meal wastewater in a stirring state, stirring for 0.5-2 min at a rotation speed of 150-200 rpm, stirring for 0.5-2 min at a rotation speed of 50-80 rpm, and filtering.
6. The use of an environmentally friendly flocculant according to claim 5, wherein: the environment-friendly flocculant is prepared into a solution of 1-10 g/L and then added into the soybean meal wastewater.
7. The use of an environmentally friendly flocculant according to claim 5, wherein: the final concentration of the environment-friendly flocculant in the soybean meal wastewater is 200-400 mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111475645.7A CN114014428B (en) | 2021-12-06 | 2021-12-06 | Environment-friendly flocculant and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111475645.7A CN114014428B (en) | 2021-12-06 | 2021-12-06 | Environment-friendly flocculant and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114014428A CN114014428A (en) | 2022-02-08 |
| CN114014428B true CN114014428B (en) | 2023-06-13 |
Family
ID=80068035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111475645.7A Active CN114014428B (en) | 2021-12-06 | 2021-12-06 | Environment-friendly flocculant and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114014428B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874445B (en) * | 2022-04-29 | 2023-09-19 | 合肥工业大学智能制造技术研究院 | Preparation method and application of dextran-polyaspartic acid anionic flocculant |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014038537A1 (en) * | 2012-09-10 | 2014-03-13 | 栗田工業株式会社 | Water treatment method and apparatus |
| CN110437378A (en) * | 2019-09-09 | 2019-11-12 | 安徽春霖环保科技有限公司 | Cationic dextran based polyalcohol, preparation and the application as flocculant |
| CN110759413B (en) * | 2019-11-01 | 2020-08-14 | 山东鑫泰水处理技术股份有限公司 | Printing and dyeing wastewater treatment agent and treatment method |
| CN112920404B (en) * | 2021-01-29 | 2023-01-24 | 合肥工业大学 | Preparation method and application of dextran-polyamino acid cationic flocculant |
-
2021
- 2021-12-06 CN CN202111475645.7A patent/CN114014428B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN114014428A (en) | 2022-02-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yang et al. | A review on chitosan-based flocculants and their applications in water treatment | |
| Salehizadeh et al. | Recent advances in polysaccharide bio-based flocculants | |
| Wu et al. | Evaluation of chain architectures and charge properties of various starch-based flocculants for flocculation of humic acid from water | |
| CN108359103B (en) | Hydrophobic modified chitosan flocculant and preparation method and application thereof | |
| CN109319908A (en) | Modification of chitosan-sodium alginate composite flocculation agent preparation method and products thereof and application | |
| KR20070116274A (en) | Use of polysaccharides in order to eliminate heavy metals in the form of anions from water | |
| Moosa et al. | Use of biocomposite adsorbents for the removal of methylene blue dye from aqueous solution | |
| CN111620473B (en) | Water treatment method | |
| CN114014428B (en) | Environment-friendly flocculant and application thereof | |
| CN112920404B (en) | Preparation method and application of dextran-polyamino acid cationic flocculant | |
| CN111573798A (en) | Natural composite polyaluminum chloride water treatment agent and preparation method thereof | |
| CN114874445B (en) | Preparation method and application of dextran-polyaspartic acid anionic flocculant | |
| CN100343180C (en) | Chitin/chitosan water processing flocculating agent and its preparing method | |
| CN114890525B (en) | Preparation method and application of dextran-betaine cationic flocculant | |
| Haleem et al. | Flocculation of livestock wastewater using cationic starch prepared from potato peels | |
| CN111573801B (en) | Organic composite aluminum sulfate water treatment agent and preparation method thereof | |
| CN1119294C (en) | Method for preparing flocculent for treatment of chitosan water | |
| CN1266821A (en) | Polyaluminium chloride and chitin compounded efficient flocculant and its preparing process | |
| CN114044564A (en) | Preparation method of dextran-based flocculant and method for clarifying fish-farming circulating water by using dextran-based flocculant | |
| Pang et al. | Synthesis and flocculation characteristics of cationic modified corncob: a novel polymeric flocculant | |
| CN108530558B (en) | Preparation method of amphoteric particle flocculant based on sodium alginate | |
| CN110921753B (en) | Ternary graft copolymerization modified spirulina protein water quality clarifying agent and preparation method thereof | |
| Rokhati et al. | The use of chitosan as non-toxic flocculant for harvesting microalgae Spirulina sp | |
| CN111717954A (en) | Heavy metal wastewater treating agent and treating method thereof | |
| KR20000047351A (en) | Coagulant for treating wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |