CN114014428A - Environment-friendly flocculating agent and application thereof - Google Patents
Environment-friendly flocculating agent and application thereof Download PDFInfo
- Publication number
- CN114014428A CN114014428A CN202111475645.7A CN202111475645A CN114014428A CN 114014428 A CN114014428 A CN 114014428A CN 202111475645 A CN202111475645 A CN 202111475645A CN 114014428 A CN114014428 A CN 114014428A
- Authority
- CN
- China
- Prior art keywords
- environment
- dextran
- friendly
- solution
- citric acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5263—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using natural chemical compounds
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
- C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Hydrology & Water Resources (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- General Chemical & Material Sciences (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
An environment-friendly flocculant and application thereof, belonging to the technical field of environment-friendly flocculants. At present, the most widely used flocculating agents are mainly inorganic flocculating agents (such as polyaluminium chloride) and synthetic organic flocculating agents (such as polyacrylamide), and the flocculating agents have certain toxicity and are difficult to degrade, secondary pollution is caused by wide use, and waste residues cannot be treated. The invention provides an environment-friendly flocculating agent which is non-toxic, harmless, free of secondary pollution and good in performance and application thereof. The preparation method comprises the following steps: adjusting the pH value of the citric acid solution to 7-9 by using sodium hydroxide, mixing and stirring the citric acid solution and the dextran solution uniformly, adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to react for 12-36 h, wherein the molar ratio of edc to citric acid is 1-2: 1; adding ethanol for precipitation, washing the precipitate, and drying to obtain the flocculant. The flocculated soybean meal waste liquid can be used for livestock breeding, and has the advantages of simple preparation and high efficiency.
Description
Technical Field
An environment-friendly flocculant and application thereof, belonging to the technical field of environment-friendly flocculants.
Background
With the development of industry, more and more industrial wastewater is generated, the problem of environmental pollution caused by the industrial wastewater is more and more severe, the flocculation method is the most widely applied sewage treatment method, the flocculation method has the advantages of high efficiency, low cost and the like, the main effect of the flocculation process is a flocculating agent, the flocculating agent which is most widely applied at present mainly comprises an inorganic flocculating agent (such as polyaluminium chloride) and a synthetic organic flocculating agent (such as polyacrylamide), although the flocculating agents have high flocculation activity and low cost, the flocculating agents have certain toxicity and are difficult to degrade, secondary pollution is caused by wide use, new environmental and human health problems are caused, and solid residues obtained after flocculation precipitation do not have a reasonable treatment method.
The processes of fermentation, washing and the like in the bean product industry generate a large amount of high-turbidity turbid wastewater which contains a large amount of oxidizable organic matters and cannot be directly discharged, but at the same time, the process also means that the wastewater contains rich nutrients such as protein, amino acid, vitamins, inorganic salt and the like, and has great resource utilization value.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: overcomes the defects of the prior art, and provides the environment-friendly flocculating agent which is non-toxic, harmless, degradable, free of secondary pollution and good in performance and the application thereof.
The technical scheme adopted by the invention for solving the technical problems is as follows: an environment-friendly flocculant is characterized in that: the main component is dextran-citric acid anion polymer.
The dextran-citric acid anion flocculant has the advantages that two natural products of high-molecular dextran and citric acid are applied to the field of flocculants, the dextran provides extremely high molecular weight, the citric acid provides enough grafting anions, the citric acid and the dextran generate higher cooperation effect, the grafting is easy, the complementation and the synergy are realized, and the final dextran-citric acid anion flocculant contains a large number of anion active groups and can be used as a biopolymer flocculant with good performance to remove impurity particles with cationic groups in a water body. Does not contain metal ions and toxic and harmful organic monomers, has good biocompatibility and biodegradability, and has wider application range.
The preparation method of the environment-friendly flocculating agent comprises the following steps: the pH value of the citric acid solution is adjusted to 7-9 by using strong base, the citric acid solution and the dextran solution are mixed and stirred uniformly, 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride is added to react for 12-36 h, and the molar ratio of the 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to the citric acid is 1-2: 1; adding ethanol for precipitation, washing the precipitate, and drying to obtain the flocculant.
The preferable mixing time of the dextran solution and the citric acid solution is 3-5 min, and the preferable reaction temperature after adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride is room temperature; the strong base is a common alkali without introducing heavy metal ions, and is preferably sodium hydroxide.
The reaction mechanism of the above reaction is shown by the following formula (1):
firstly, preparing macromolecular dextran by using dextran sucrase to catalyze sucrose reaction, then using 1- (3-dimethylaminopropyl) -3-ethyl carbodiimide hydrochloride (EDC) as an activating reagent, and using esterification reaction between the macromolecular dextran and citric acid to link citric acid to dextran molecular chains to obtain the anionic dextran-based flocculant.
The invention creatively applies two natural products of macromolecular dextran and citric acid to the field of flocculant, utilizes esterification reaction of the macromolecular dextran and citric acid to perform derivatization of dextran to obtain the dextran-citric acid anion flocculant, dextran provides extremely high molecular weight, citric acid provides enough grafting anions, citric acid and dextran generate higher coordination effect, and the dextran-citric acid anion flocculant is easy to graft and simultaneously has complementary synergy, and the final dextran-citric acid anion flocculant contains a large amount of anion active groups and can be used as a biopolymer flocculant with good performance for removing impurity particles with cationic groups in water. The preparation process and the product are nontoxic, harmless and degradable, the raw material source is wide, the derivatization method is mild and safe, the operation process steps are simple, the product quality is controllable, and the prepared flocculating agent does not contain metal ions and toxic and harmful organic monomers, has good biocompatibility and biodegradability, and does not bring harm to human health and environmental safety. Meanwhile, the flocculating agent is high in flocculating performance, small in using amount, applicable to pH 4-9, more thorough in sedimentation, and capable of being matched with other cationic flocculating agents such as chitosan to be used for treating the sedimentation of wastewater in the industries of hematite and bean products, and the flocculating effect is obviously improved.
Preferably, the concentration of the dextran solution is 10-60 g/L.
The preferable concentration can fully react with the citric acid solution and obtain higher concentration of the dextran-citric acid anion type flocculating agent, thus being easy for final precipitation purification.
Preferably, the dextran in the dextran solution is obtained by enzyme-catalyzed reaction of dextran sucrase in sucrose solution.
The dextransucrase is a hydrolytic synthetase for synthesizing carbohydrate catalyzed and synthesized by taking sucrose as a substrate, can be produced by leuconostoc mesenteroides, can be produced by genetic engineering bacteria capable of highly expressing dextransucrase as described in Chinese patent CN101363009A, and can also be produced by mixed fermentation as described in Chinese patent CN 105132390A.
The dextran obtained by adopting the optimized dextran preparation method has higher activity and higher controllability, so that the dextran with higher molecular weight can be catalytically synthesized under the condition suitable for process control, thereby obtaining the dextran-citric acid anion type flocculant with higher flocculation efficiency and less consumption, and being more suitable for the preparation method of the environment-friendly flocculant.
Preferably, the enzyme catalysis reaction comprises preparing a sucrose solution from an acetic acid-calcium acetate buffer solution with a pH value of 5.0-5.5, adding dextran sucrase into the sucrose solution to perform an enzyme catalysis reaction, reacting at 20-28 ℃ until the dynamic viscosity reaches 8000-10000 CPS, stopping the reaction, precipitating high molecular weight dextran with ethanol, and washing to obtain the dextran.
If the obtained dextran is dissolved in water, a high-molecular dextran solution can be obtained, and if the dextran is placed in a constant-temperature air blast oven at the temperature of 30-50 ℃ for drying and grinding, dextran solid powder can be finally obtained. In the preparation process of the environment-friendly flocculant, the dextran is easier to participate in the reaction in a solution mode, so that the reaction efficiency is improved.
Further preferably, the molar concentration of the sucrose solution is 0.55-0.75 mol/L, and the dextran sucrase is a solution with a concentration of 5.0-6.0U/mL.
Preferably, after the mixing and stirring are carried out uniformly, the molar ratio of the citric acid to the dextran anhydroglucose units in the solution is 0.1-1.0: 1.
the application of the environment-friendly flocculant is characterized in that: the environment-friendly flocculating agent is added into the soybean meal wastewater in a stirring state, stirred for 0.5-2 min at the rotating speed of 150-200 rpm, stirred for 0.5-2 min at the rotating speed of 50-80 rpm, and filtered.
The environment-friendly flocculant contains a large amount of anionic active groups, is suitable for removing impurity particles with cationic groups in the bean pulp wastewater, flocculates and settles the impurity particles such as protein, amino acid and the like in the bean pulp wastewater through flocculation mechanism effects such as charge neutralization, adsorption bridging and the like to play a good clarification role, and is also suitable for the fields of other foods, medicines and the like in the same way; in addition, by utilizing the characteristics of no toxicity, no harm and environmental protection, the flocculated solid sediment can be used in the fields of livestock feeding and the like, so that the application value is obtained again, the double treatment of waste water and solid waste is realized, the economic benefit is obtained, and the environment is protected.
Further preferably, the stirring speed of the environment-friendly flocculating agent in the adding process is 150-200 rpm.
The whole adding process is divided into a mode of firstly quickly stirring and then switching to slowly stirring, the optimized rotating speed is more suitable for fully combining and reacting anions and cations at the initial stage of flocculation, and at the later stage of flocculation, the flocculation structure is protected, full sedimentation is ensured, and therefore a better flocculation effect is achieved.
Preferably, the environment-friendly flocculating agent is added into the soybean meal waste liquid after being prepared into 1-10 g/L solution.
The environment-friendly flocculant is prepared into mother liquor and then participates in flocculation reaction, so that the dispersion effect of the flocculant can be effectively improved, impurities in waste liquid can be ensured to fully contact with the anionic dextran-based flocculant solution, and the flocculation effect is improved.
Preferably, the concentration of the final environment-friendly flocculating agent in the soybean meal wastewater is 200-400 mg/L.
The optimized flocculant dosage can fully settle most of impurities such as protein, amino acid and the like in the bean pulp wastewater, so that excessive anionic groups can be effectively prevented from being introduced, the flocculation effect is ensured, the flocculant dosage is saved, and the impurities in the treated wastewater are reduced.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, citric acid is grafted to a polymer dextran chain through an esterification reaction, so that a natural anionic dextran-based flocculant is successfully prepared, raw materials used in the preparation are all derived from natural products, and the flocculant has the advantages of environmental friendliness, no toxicity, no harm, wide source and the like, the adopted derivatization method is mild and safe, the operation process steps are simple, the product quality is controllable, and the prepared flocculant does not contain metal ions and toxic and harmful organic monomers, has good biocompatibility and biodegradability, and does not bring harm to human health and environmental safety. Compared with the inorganic and synthetic organic flocculants sold in the market at present, the dextran-citric acid anion flocculant is safe and environment-friendly, has no secondary pollution, does not have the problems of metal residue, pipeline corrosion, toxic monomer residue and the like, has a wider application range, and can be used for clarification processes in the fields of food, medicine and the like.
Drawings
FIG. 1 is a graph of the results of a flocculation experiment performed on a hematite suspension simulated wastewater sample in example 1.
Detailed Description
The present invention is further illustrated by the following examples, example 1 being the best mode of carrying out the invention.
Example 1
A dextran-citric acid anion type environment-friendly flocculant is prepared by the following steps:
1) preparing a 0.58mol/L sucrose solution by adopting an acetic acid-calcium acetate buffer solution with pH =5.4, and adding a dextran sucrase solution into the sucrose solution, wherein the enzyme concentration is 5.0U/mL; carrying out enzyme catalysis reaction for 24 hours in a shaker at 25 ℃ and 100 rpm; the dynamic viscosity reaches 9000CPS at the end of the reaction; precipitating a high molecular weight dextran product by using ethanol, washing for 2 times, and drying in a constant-temperature blast oven at 40 ℃; grinding the high molecular weight dextran product after drying to obtain high molecular weight dextran solid powder;
2) preparing a high molecular weight dextran solution and a citric acid solution, adjusting the pH value of the citric acid solution to 7.5 by using a sodium hydroxide solution, mixing and stirring the solution with the dextran solution for 5min, wherein the concentration of dextran in the mixed solution is 25g/L, and the molar ratio of citric acid to dextran anhydroglucose units is 0.5: 1; adding EDC, wherein the molar ratio of EDC to citric acid is 1: 1; reacting the reaction solution at room temperature for 24h, adding absolute ethyl alcohol to precipitate a product, and washing the product for 2 times by using 80% ethyl alcohol; and (3) drying and grinding the product in a constant-temperature blast oven at 40 ℃ to obtain white dry solid powder, namely the anionic dextran-based flocculant.
Example 2
Based on example 1, the dextran concentration of 50g/L is set in the mixed solution in step 2), and the molar ratio of the final citric acid to the dextran anhydroglucose unit is 1:1, other conditions were the same as in example 1.
Example 3
Based on example 1, the dextran concentration of 10g/L is set in the mixed solution in step 2), and the final molar ratio of citric acid to dextran anhydroglucose units is 0.1: 1, other conditions were the same as in example 1.
Example 4
A dextran-citric acid anionic environment-friendly flocculant, based on example 1, EDC is added in step 2) so that the molar ratio of EDC to citric acid is 2: 1; other conditions were the same as in example 1.
Example 5
An application of dextran-citric acid anion type environment-friendly flocculant, the anion type dextran-based flocculant obtained in example 1 is used for clarifying bean pulp waste liquid and recycling filter residue. The following method is adopted:
preparing the dextran-citric acid anion type environment-friendly flocculant prepared in the example 1 into 1g/L flocculant solution, adding the flocculant solution into the soybean meal waste liquid at the rotating speed of 200rpm until the concentration of the dextran-citric acid anion type environment-friendly flocculant in the soybean meal waste liquid is 300mg/L, continuously stirring for 1min, and stirring for 1min after the rotating speed is reduced to 80 rpm; stopping stirring, standing for settling, filtering, and finishing the treatment.
After the clarification of the method, the turbidity of the soybean meal waste liquid is greatly reduced, the water body becomes clear, the original suspended particles form lumpy flocculate under the flocculation effect, the flocculate is settled under the gravity effect, and after the flocculate is filtered and washed, no heavy metal or sulfur cyanide monomer is detected, so the method can be used for livestock feeding.
Example 6
Based on example 5, the dextran-citric acid anion type environment-friendly flocculant is added into soybean meal waste liquid, the concentration of the dextran-citric acid anion type environment-friendly flocculant is 200mg/L, and other conditions are the same as example 5.
After the clarification of the method, the turbidity of the soybean meal waste liquid is greatly reduced, the water body becomes clear, the original suspended particles form lumpy flocculate under the flocculation effect, the flocculate is settled under the gravity effect, and after the flocculate is filtered and washed, no heavy metal or sulfur cyanide monomer is detected, so the method can be used for livestock feeding.
Comparative example 1
A flocculant, namely a commercial flocculant polyferric sulfate, can achieve more than 90 percent of protein recovery rate of soybean meal waste liquid, but the polyferric sulfate as an inorganic flocculant can pollute the recovered protein, the recovered protein cannot be used as feed, the resource utilization of waste cannot be realized, and the flocculated waste liquid contains a large amount of metal ion residues, so that secondary pollution is easily caused.
Comparative example 2
A dextran-citric acid anion type environment-friendly flocculant is prepared by the following steps:
utilizing the high molecular weight dextran solid powder obtained in the embodiment 1 to prepare a high molecular weight dextran solution and a citric acid solution, mixing and stirring the citric acid solution and the dextran solution for 5min, wherein the concentration of dextran in the mixed solution is 25g/L, and the molar ratio of citric acid to dextran anhydroglucose units is 1: 1; adding sodium hypophosphite (PHP) with the mass ratio of 1:10 to the citric acid; placing the reaction solution in a water bath kettle at 60 ℃ for reaction for 5 hours, adding absolute ethyl alcohol after the reaction is finished, precipitating to obtain a product, and washing for 2 times by using 80% ethyl alcohol; and (3) drying and grinding the product in a constant-temperature blast oven at 40 ℃ to obtain white dry solid powder, namely the anionic dextran-based flocculant.
Comparative example 3
A dextran-citric acid anion type environment-friendly flocculant is prepared by the following steps:
utilizing the high molecular weight dextran solid powder obtained in the embodiment 1 to prepare a high molecular weight dextran solution and a citric acid solution, adjusting the pH value of the citric acid solution to 4 by using a sodium hydroxide solution, mixing and stirring the solution with the dextran solution for 5min, wherein the concentration of dextran in the mixed solution is 25g/L, and the molar ratio of citric acid to dextran anhydroglucose units is 0.5: 1; adding EDC, wherein the molar ratio of EDC to citric acid is 1: 1; reacting the reaction solution at room temperature for 24h, adding absolute ethyl alcohol to precipitate a product, and washing the product for 2 times by using 80% ethyl alcohol; and (3) drying and grinding the product in a constant-temperature blast oven at 40 ℃ to obtain white dry solid powder, namely the anionic dextran-based flocculant.
Comparative example 4
A dextran-citric acid anion type environment-friendly flocculant is prepared by the following steps:
utilizing the high molecular weight dextran solid powder obtained in the embodiment 1 to prepare a high molecular weight dextran solution and a malic acid solution, adjusting the pH value of the malic acid solution to 7 by using a sodium hydroxide solution, mixing and stirring the malic acid solution and the dextran solution for 5min, wherein the concentration of dextran in the mixed solution is 25g/L, and the molar ratio of malic acid to dextran anhydroglucose units is 1: 1; adding EDC, wherein the molar ratio of EDC to malic acid is 1: 1; reacting the reaction solution at room temperature for 24h, adding absolute ethyl alcohol to precipitate a product, and washing the product for 2 times by using 80% ethyl alcohol; and (3) drying and grinding the product in a constant-temperature blast oven at 40 ℃ to obtain white dry solid powder, namely the anionic dextran-based flocculant.
Performance testing
0.25g of hematite powder is weighed and stirred in 250mL of distilled water for 5min to obtain a hematite suspension simulated wastewater sample with the concentration of 1.0 g/L.
Dissolving the dextran-citric acid anion type environment-friendly flocculant powder prepared in the examples 1-4 and the comparative examples 1 and 2 in distilled water, reconfiguring the obtained solution into dextran-citric acid anion type environment-friendly flocculant mother liquor with the concentration of 1mg/L, adding 2ml of the prepared dextran-citric acid anion flocculant mother liquor into the hematite suspension, stirring while adding, standing for 2min after stirring for 2min, taking the supernatant, detecting the absorbance value at 550nm, and calculating the flocculation efficiency F% according to the following formula:
F%=（F0-F1）/ F0 * 100%
wherein F0 and F1 are absorbance values of the hematite suspension at 550nm before and after flocculation, respectively.
The final flocculation efficiencies F% of examples 1-4 were 84.4%, 83.5%, 82.7%, 82.5%, respectively, at a pH of 6 for the hematite suspension; the final flocculation efficiencies F% of comparative examples 1-4 were 90.1%, 70.5%, 61.1%, 60.9%, respectively.
The experimental results show that the change trends of the flocculation efficiency are similar when the dosage of the dextran-citric acid anion type environment-friendly flocculant obtained in example 1 is increased from 0mg/L to 72mg/L under different system pH values of 4-9, the change trends of the flocculation efficiency are firstly rapidly increased when the dosage is only 8mg/L, a higher flocculation efficiency is reached, then the increase rate is slowed, and the flocculation efficiency is gradually decreased after the highest value is reached, wherein the dosage of the dextran-citric acid anion type environment-friendly flocculant is increased from 0mg/L to 72mg/L under different system pH values in the range of 4-9. At pH 6, the optimum dosage was the lowest, corresponding to the highest flocculation efficiency, 24mg/L and 84.4%, respectively. At pH4, the maximum flocculation efficiency was still 70% although it was low, and it was found that the dextran-citric acid anion flocculant of the present invention has excellent flocculation activity in a wide pH range.
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (10)
1. An environment-friendly flocculant is characterized in that: the composition comprises a dextran-citric acid anionic polymer.
2. The environment-friendly flocculant according to claim 1, characterized in that: the preparation method comprises the following steps: the pH value of the citric acid solution is adjusted to 7-9 by using strong base, the citric acid solution and the dextran solution are mixed and stirred uniformly, 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride is added to react for 12-36 h, and the molar ratio of the 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to the citric acid is 1-2: 1; adding ethanol for precipitation, washing the precipitate, and drying to obtain the flocculant.
3. The environment-friendly flocculant according to claim 2, characterized in that: the concentration of the dextran solution is 10-60 g/L.
4. The environment-friendly flocculant according to claim 2, characterized in that: the dextran in the dextran solution is obtained by enzyme catalytic reaction of dextran sucrase in sucrose solution.
5. The environment-friendly flocculant according to claim 4, characterized in that: the enzyme catalysis reaction comprises the steps of preparing a sucrose solution from an acetic acid-calcium acetate buffer solution with the pH value of 5.0-5.5, adding dextran sucrase into the sucrose solution to perform enzyme catalysis reaction, reacting at the temperature of 20-28 ℃ until the dynamic viscosity reaches 8000-10000 CPS, stopping the reaction, precipitating high molecular weight dextran with ethanol, and washing to obtain the dextran.
6. The environment-friendly flocculant according to claim 5, characterized in that: the molar concentration of the sucrose solution is 0.55-0.75 mol/L, and the dextran sucrase is a solution with the concentration of 5.0-6.0U/mL.
7. The environment-friendly flocculant according to claim 2, characterized in that: after the mixing and stirring are uniform, the molar ratio of the citric acid to the dextran anhydroglucose units in the solution is 0.1-1.0: 1.
8. the application of the environment-friendly flocculant is characterized in that: the environment-friendly flocculating agent of any one of claims 1 to 7 is added into the soybean meal wastewater in a stirring state, stirred for 0.5 to 2min at the rotating speed of 150 to 200rpm, stirred for 0.5 to 2min at the rotating speed of 50 to 80rpm, and filtered.
9. The use of the environment-friendly flocculant according to claim 8, wherein the environment-friendly flocculant comprises the following components: the environment-friendly flocculating agent is added into the soybean meal waste liquid after being prepared into 1-10 g/L solution.
10. The use of the environment-friendly flocculant according to claim 8, wherein the environment-friendly flocculant comprises the following components: the final concentration of the environment-friendly flocculating agent in the soybean meal wastewater is 200-400 mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111475645.7A CN114014428B (en) | 2021-12-06 | 2021-12-06 | Environment-friendly flocculant and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111475645.7A CN114014428B (en) | 2021-12-06 | 2021-12-06 | Environment-friendly flocculant and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114014428A true CN114014428A (en) | 2022-02-08 |
| CN114014428B CN114014428B (en) | 2023-06-13 |
Family
ID=80068035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111475645.7A Active CN114014428B (en) | 2021-12-06 | 2021-12-06 | Environment-friendly flocculant and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114014428B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874445A (en) * | 2022-04-29 | 2022-08-09 | 合肥工业大学智能制造技术研究院 | Preparation method and application of dextran-polyaspartic acid anion flocculant |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014038537A1 (en) * | 2012-09-10 | 2014-03-13 | 栗田工業株式会社 | Water treatment method and apparatus |
| CN110437378A (en) * | 2019-09-09 | 2019-11-12 | 安徽春霖环保科技有限公司 | Cationic dextran based polyalcohol, preparation and the application as flocculant |
| CN110759413A (en) * | 2019-11-01 | 2020-02-07 | 临沂舜驰生态科技有限公司 | Printing and dyeing wastewater treatment agent and treatment method |
| CN112920404A (en) * | 2021-01-29 | 2021-06-08 | 合肥工业大学 | Preparation method and application of dextran-polyamino acid cationic flocculant |
-
2021
- 2021-12-06 CN CN202111475645.7A patent/CN114014428B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014038537A1 (en) * | 2012-09-10 | 2014-03-13 | 栗田工業株式会社 | Water treatment method and apparatus |
| CN110437378A (en) * | 2019-09-09 | 2019-11-12 | 安徽春霖环保科技有限公司 | Cationic dextran based polyalcohol, preparation and the application as flocculant |
| CN110759413A (en) * | 2019-11-01 | 2020-02-07 | 临沂舜驰生态科技有限公司 | Printing and dyeing wastewater treatment agent and treatment method |
| CN112920404A (en) * | 2021-01-29 | 2021-06-08 | 合肥工业大学 | Preparation method and application of dextran-polyamino acid cationic flocculant |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874445A (en) * | 2022-04-29 | 2022-08-09 | 合肥工业大学智能制造技术研究院 | Preparation method and application of dextran-polyaspartic acid anion flocculant |
| CN114874445B (en) * | 2022-04-29 | 2023-09-19 | 合肥工业大学智能制造技术研究院 | Preparation method and application of dextran-polyaspartic acid anionic flocculant |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114014428B (en) | 2023-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yang et al. | A review on chitosan-based flocculants and their applications in water treatment | |
| Salehizadeh et al. | Recent advances in polysaccharide bio-based flocculants | |
| Jiang et al. | Biopolymer-based flocculants: A review of recent technologies | |
| US5433865A (en) | Method for treating process waste streams by use of natural flocculants | |
| Loganathan et al. | Progress, challenges, and opportunities in enhancing NOM flocculation using chemically modified chitosan: a review towards future development | |
| Cheng et al. | Using chitosan as a coagulant in recovery of organic matters from the mash and lauter wastewater of brewery | |
| Bough et al. | Influence of manufacturing variables on the characteristics and effectiveness of chitosan products. I. Chemical composition, viscosity, and molecular‐weight distribution of chitosan products | |
| Sila et al. | Chitin and chitosan extracted from shrimp waste using fish proteases aided process: efficiency of chitosan in the treatment of unhairing effluents | |
| CN108359103B (en) | Hydrophobic modified chitosan flocculant and preparation method and application thereof | |
| Moosa et al. | Use of biocomposite adsorbents for the removal of methylene blue dye from aqueous solution | |
| KR20070116274A (en) | Use of polysaccharides in order to eliminate heavy metals in the form of anions from water | |
| Abdo et al. | Cationic starch and polyaluminum chloride as coagulants for River Nile water treatment | |
| CN109319908A (en) | Modification of chitosan-sodium alginate composite flocculation agent preparation method and products thereof and application | |
| Cui et al. | Microwave assisted synthesis and characterization of a ternary flocculant from chitosan, acrylamide and lignin | |
| Sudha et al. | Marine carbohydrates of wastewater treatment | |
| CN114014428B (en) | Environment-friendly flocculant and application thereof | |
| Hatta et al. | Novel cationic chitosan-like bioflocculant from Citrobacter youngae GTC 01314 for the treatment of kaolin suspension and activated sludge | |
| Sudha et al. | Applications of chitin/chitosan and its derivatives as adsorbents, coagulants and flocculants | |
| CN112920404B (en) | Preparation method and application of dextran-polyamino acid cationic flocculant | |
| Feng et al. | Synthesis of a chitosan-based flocculant CS-gP (AM-IA-AATPAC) and evaluation of its performance on Ni2+ removal: Role of chelating-coordination and flocculation | |
| Hatta et al. | Parametric and kinetic studies of activated sludge dewatering by cationic chitosan-like bioflocculant BF01314 produced from Citrobacter youngae | |
| CN114874445B (en) | Preparation method and application of dextran-polyaspartic acid anionic flocculant | |
| Shao et al. | Study on the influencing factors of natural pectin's flocculation: Their sources, modification, and optimization | |
| CN114890525B (en) | Preparation method and application of dextran-betaine cationic flocculant | |
| Yang et al. | Polyacrylamide grafted cellulose as an eco-friendly flocculant: efficient removal of organic dye from aqueous solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |