CN114013827B - Self-maintaining liquid nitrogen type biological preservation container - Google Patents
Self-maintaining liquid nitrogen type biological preservation container Download PDFInfo
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- CN114013827B CN114013827B CN202111255171.5A CN202111255171A CN114013827B CN 114013827 B CN114013827 B CN 114013827B CN 202111255171 A CN202111255171 A CN 202111255171A CN 114013827 B CN114013827 B CN 114013827B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/38—Devices for discharging contents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
- B65D81/20—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
- B65D81/2069—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas in a special atmosphere
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/38—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
- B65D81/3802—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container in the form of a barrel or vat
- B65D81/3806—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container in the form of a barrel or vat formed with double walls, i.e. hollow
Abstract
The invention provides a self-maintaining liquid nitrogen type biological preservation container. The invention comprises a liquid nitrogen tank main body, a Stirling unit and a liquid nitrogen replenishing system, wherein the liquid nitrogen tank main body comprises a liquid nitrogen storage tank, an inner container sleeved outside the liquid nitrogen storage tank and an outer container sleeved outside the inner container, a sampling port for storing biological samples is arranged in a space between the inner container and the outer container, a port of the Stirling unit is connected with the top end of the liquid nitrogen storage tank, the liquid nitrogen storage tank is connected with the liquid nitrogen replenishing system, a valve is arranged between the liquid nitrogen storage tank and the liquid nitrogen replenishing system, the Stirling unit is used for condensing volatilized nitrogen into liquid nitrogen, a pressure sensor is arranged on a storage tank exhaust connecting pipe connected with the liquid nitrogen storage tank, a temperature sensor is arranged on the inner container, the pressure sensor and the temperature sensor are both connected with a controller, and the controller is connected with the valve. The invention breaks the traditional refrigeration mode of long-term liquid nitrogen replenishment, and can realize the deep low-temperature storage environment of liquid nitrogen by being provided with the automatic liquid adding mode of the liquid nitrogen replenishment tank.
Description
Technical Field
The invention relates to preservation of liquid nitrogen biological samples, in particular to a self-maintaining liquid nitrogen biological preservation container.
Background
The liquid nitrogen biological preservation container produced in the current market uses liquid nitrogen as a refrigeration source, the deep low-temperature environment in the tank is maintained by virtue of low temperature caused by natural volatilization of the liquid nitrogen, and the liquid nitrogen quantity in the tank is gradually reduced along with the consumption of the liquid nitrogen, so that the liquid nitrogen is required to be periodically supplied. Some remote areas or high-end security scenes are inconvenient for periodic replenishment of liquid nitrogen.
The liquid nitrogen tank in the current market all need rely on traditional liquid nitrogen supply tank or pipeline to carry out the periodic supply of liquid nitrogen, and the inconvenience of bringing like this is as follows: 1. requiring customers to have a long-term stable liquid nitrogen supplier; 2. remote areas have no liquid nitrogen supply channels; 3. the replenishment tank needs to be replaced frequently, and a large number of people enter a sample management area frequently; 4. the scientific research and confidentiality organization does not allow the ingress and egress of external personnel; 5. most of liquid nitrogen replenishing tanks are pressure vessels, recording is needed, and the use is extremely inconvenient; 6. operators are at risk of contact with liquid nitrogen, such as dangerous accidents of liquid nitrogen overflow and the like; 7. the liquid adding electromagnetic valve, the liquid level meter and the like are all vulnerable parts, and the failure rate is high. 8. For countries and regions with multiple earthquakes, the first energy recovered after an earthquake is electric energy, so a container for maintaining the temperature of liquid nitrogen by using electric energy is highly desirable
Disclosure of Invention
According to the technical problems set forth above, a self-sustaining liquid nitrogen type biological preservation container is provided. The invention can realize the liquid nitrogen self-supplying liquid nitrogen tank by means of electric energy (by means of Stirling closed cycle refrigeration), not only can solve the pain point of a user end, but also breaks through the traditional refrigeration mode of long-term liquid nitrogen supplying, realizes the innovation of the liquid nitrogen tank industry, and adds bricks and tiles for the high and new technology in China. The invention adopts the following technical means:
the utility model provides a self-sustaining liquid nitrogen formula biological preservation container, includes liquid nitrogen tank main part, stirling unit and liquid nitrogen replenishment system, the liquid nitrogen tank main part includes the liquid nitrogen storage tank, cup joints the outer container of inner container and cup joints outside the inner container outside the liquid nitrogen storage tank, be equipped with the material taking mouth that is used for preserving biological sample in the space between inner container and the outer container, the Stirling unit passes through flange joint on the liquid nitrogen tank main part, the port of Stirling unit links to each other with the top of liquid nitrogen storage tank, the liquid nitrogen storage tank links to each other with the liquid nitrogen replenishment system, be equipped with the valve therebetween, the liquid nitrogen replenishment system is used for supplying preset volume's liquid nitrogen into the liquid nitrogen storage tank in, be equipped with pressure sensor on the storage tank exhaust take over that Stirling unit and liquid nitrogen storage tank are connected, be equipped with temperature sensor on the inner container, pressure sensor and temperature sensor all link to each other with the controller, the controller link to each other with the valve, the controller still link to each other with Stirling unit, after the controller is used for judging that the liquid nitrogen surplus reaches the relation of setting value or storage space's temperature value and setting value.
Further, the outer container comprises an outer container cylinder body, an outer container lower end socket, an outer container upper end socket, truckles and an inner and outer container support, wherein the lower part of the outer container cylinder body is connected with the outer container lower end socket, the upper part of the outer container cylinder body is connected with the outer container upper end socket, the truckles are connected with the lower part of the outer container lower end socket, and the inner and outer container support for fixing the inner container is arranged on the outer container lower end socket.
Further, the inner container support and the outer container support comprise an outer container lower end socket support piece and reinforcing rib plates connected with the outer container lower end socket support piece, the upper end of the outer container lower end socket support piece is connected with the inner lower end socket support, and a glass fiber reinforced plastic heat insulation layer is arranged between the upper end of the outer container lower end socket support piece and the inner lower end socket support.
Further, the inner container includes inner container barrel, inner container lower cover, inner container upper cover, storage tank lower support, interior body and interior body rotating member, the below of inner container barrel is connected the inner container lower cover, the top is connected the inner container upper cover, set up the interior lower cover internal rotation body support that is used for installing interior body on the inner container lower cover, be equipped with on the interior lower cover internal rotation body support and be used for fixing the storage tank lower support of liquid nitrogen storage tank, interior body passes through interior body rotating member and connects the outside of liquid nitrogen storage tank.
Further, the valve is an electromagnetic valve, and when the temperature detected by the temperature sensor is not set, the liquid nitrogen replenishing system conveys liquid nitrogen into the liquid nitrogen storage tank through a self-pressurization principle; when the temperature detected by the temperature sensor reaches a set value, the electromagnetic valve is closed.
Further, the Stirling unit comprises a cold head, a heat radiation supporting plate, a Stirling machine, a heat radiation fan, a radiator, a knife edge flange and a shock absorber, wherein the cold head is connected with the Stirling machine, the bottom end of the cold head is assembled with a liquid nitrogen tank body through the knife edge flange, an oxygen-free copper gasket used for sealing is arranged between the cold head and the liquid nitrogen tank body, the heat radiation supporting plate is arranged on the space where the Stirling machine is located, the heat radiation fan and the radiator are arranged on the heat radiation supporting plate in preset quantity and preset positions, and the shock absorber is arranged between the two knife edge flanges.
Further, the vacuum pumping treatment is carried out between the inner container and the outer container, a vacuum interlayer is arranged between the inner container and the outer container, a liquid adding pipe of the liquid nitrogen replenishing system is arranged in the area, and a vacuum plug is arranged on the outer container.
Further, the outer surface of the inner container is provided with a heat insulating material.
Further, the pressure detection port where the pressure sensor is located is connected with a safety valve communicated with the outside, when the pressure of the liquid nitrogen storage tank is too high, the safety valve is automatically opened, the tank body is protected, and when the pressure is relieved to a safety value, the safety valve is automatically closed.
Further, the liquid adding pipe of the liquid nitrogen replenishing system is also connected with a manual liquid adding port.
The invention has the following advantages:
1. for remote areas such as inconvenient liquid nitrogen transportation, the refrigeration of liquid nitrogen can be realized only by adding liquid nitrogen and long-term electric energy at one time, and a lower and more stable deep low-temperature storage scene is provided.
2. The waiting time of filling and transporting the liquid nitrogen replenishing tank is not needed, and manpower and material resources are saved.
3. The double-selection possibility is provided, and for the scene of insufficient electric energy and convenient and fast liquid nitrogen transportation, the liquid nitrogen deep low-temperature storage environment is realized by the automatic liquid adding mode of the liquid nitrogen replenishing tank.
4. The intelligent liquid nitrogen refrigerating system is reliable in structure, can be applied to other environments using liquid nitrogen for refrigerating, such as the fields of aviation detectors, unmanned aerial vehicles and the like, and accords with the concept of intelligent development.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to the drawings without inventive effort to a person skilled in the art.
Fig. 1 is a schematic view of the external structure of the present invention.
Fig. 2 is a cross-sectional view of a front view of the internal structure of the present invention.
Fig. 3 is a section A-A of fig. 2.
Fig. 4 is a top view of the present invention.
Fig. 5 is a sectional view of B-B of fig. 2.
In the figure: 111. the inner lower seal head is supported; 112. a lower end enclosure of the inner container; 113. an inner swivel support of the inner lower seal head; 12. an inner container cylinder; 131. a tank lower support; 132. a storage tank cylinder; 133. a storage tank exhaust connection pipe; 141. an inner swivel outer cylinder; 142. an inner swivel lower rotating member; 143. a rotating member on the inner swivel; 144. An inner cylinder of the inner rotator; 145. an inner rotor separator; 151. an upper end enclosure of the inner container; 152. the object taking opening is contracted downwards; 153. a material taking port; 154. the object taking opening is contracted; 155. a fetching port cover; 156. a sleeve; 157. A knife edge flange; 211. a glass fiber reinforced plastic heat insulation layer; 212. an outer container lower head support; 213. reinforcing rib plates; 214. casters; 215. an outer container lower end socket; 22. vacuum plugging; 23. an upper end socket of the outer container; 24. an outer container cylinder; 25. a heat insulating material; 26. vacuum interlayer; 31. a cold head; 32. a heat dissipation support plate; 33. stirling machine (model TC 4187); 34. a blower; 35. a heat sink; 36. a knife edge flange; 37. a damper; 38. an oxygen-free copper gasket; 41. a temperature sensor assembly; 42. a pressure sensor assembly; 421. a pressure detection port; 422. a pipe clamp; 423. a pressure sensor; 424. a safety valve; 43. A manual liquid adding port; 431. a liquid adding tube; 432. a stop valve; 433. a liquid adding pipe clamp; 434. a liquid adding port; 5. controller (model CT 5505).
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1 to 4, the embodiment of the invention discloses a self-maintaining liquid nitrogen type biological preservation container, which comprises a liquid nitrogen tank main body, a stirling set and a liquid nitrogen replenishing system, wherein the liquid nitrogen tank main body comprises a liquid nitrogen storage tank, an inner container sleeved outside the liquid nitrogen storage tank and an outer container sleeved outside the inner container, a material taking port for preserving biological samples is arranged in a space between the inner container and the outer container, the stirling set is connected to the liquid nitrogen tank main body through a flange, a port of the stirling set is connected with the top end of the liquid nitrogen storage tank, the liquid nitrogen storage tank is connected with the liquid nitrogen replenishing system, a valve is arranged between the liquid nitrogen storage tank and the liquid nitrogen replenishing system, the liquid nitrogen replenishing system is used for supplying a preset amount of liquid nitrogen into the liquid nitrogen storage tank, a pressure sensor 423 is arranged on a storage tank exhaust connecting pipe 133 connected with the liquid nitrogen storage tank of the stirling set, a temperature sensor 41 is arranged on the inner container, the pressure sensor and the temperature sensor are both connected with a controller 5, and the controller is further connected with the stirling set for judging that the liquid nitrogen allowance or the temperature value of the storage space reaches a set value or the relation of the set value and the set value, and then the stirling set is controlled to be turned on and off. The upper end enclosure of the 151 inner container is assembled and welded with the lower necking 152 of the object taking opening, the object taking opening 153, the upper necking 154 of the object taking opening, the cover 155 of the object taking opening, the sleeve 156 and the knife edge flange 157 to form an inner upper end enclosure assembly.
The outer container comprises an outer container cylinder 24, an outer container lower seal head 215, an outer container upper seal head 23, truckles 214 and an inner and outer container support, wherein the lower part of the outer container cylinder is connected with the outer container lower seal head, the upper part of the outer container cylinder is connected with the outer container upper seal head, the lower part of the outer container lower seal head is connected with the truckles, and the inner and outer container support for fixing the inner container is arranged on the outer container lower seal head. The inner and outer container supports comprise an outer container lower end socket support 212 and reinforcing ribs 213 connected with the outer container lower end socket support, the upper end of the outer container lower end socket support is connected with the inner lower end socket support 111, and a glass fiber reinforced plastic heat insulation layer 211 is arranged between the upper end of the outer container lower end socket support and the inner lower end socket support.
The inner container comprises an inner container cylinder 12, an inner container lower seal head 112, an inner container upper seal head 151, a storage tank lower support 131, an inner rotator and an inner rotator rotating member, wherein the lower part of the inner container cylinder is connected with the inner container lower seal head, the upper part of the inner container cylinder is connected with the inner container upper seal head, an inner lower seal head inner rotator support 113 for installing an inner rotator is arranged on the inner container lower seal head, a storage tank lower support for fixing a liquid nitrogen storage tank 132 is arranged on the inner lower seal head inner rotator support, the inner rotator comprises an inner rotator inner cylinder 144 and an inner rotator outer cylinder 141, the inner rotator inner cylinder 144 is connected with the outer part of the liquid nitrogen storage tank through an inner rotator upper rotating member 143 and an inner rotator lower rotating member 142, and the inner rotator can be manually rotated by taking a sleeve 156 as a center. In this embodiment, the region between the inner rotor cylinder and the outer rotor cylinder is divided into 5 regions (A, B, C, D, E) by 5 inner rotor separators 145, and region identifiers are made on the separators, and each region can store biological samples individually, as shown in fig. 5. When the warehouse-in and warehouse-out operation is performed, the cover 155 of the object taking opening is opened, a hand stretches into the inner rotator to stir the partition 145 until a storage area is found, the warehouse-in and warehouse-out operation is performed, after the operation is finished, the cover plate is covered, and in other alternative embodiments, the number of the inner rotator partition 145 is set according to requirements. The inner lower seal head support 111, the inner lower seal head 112 and the inner lower seal head inner swivel support are assembled and welded to form an inner container lower seal head assembly; the tank lower support 131 is combined with the tank cylinder 132 and the tank vent connection pipe to form a liquid nitrogen tank assembly.
Placing the liquid nitrogen storage tank assembly on the lower end socket assembly of the inner container of the workpiece, completing welding, and assembling the inner container barrel 12 and the inner rotating body of the workpiece, so that the inner rotating body can freely rotate; and installing the inner upper seal head assembly, finishing welding, cleaning the outer surface, and winding a heat insulating material on the outer surface of the inner container to form a physical heat insulating layer, thereby forming the inner container.
The liquid nitrogen replenishing system comprises a liquid adding pipe 431 and a valve connected to the liquid adding pipe 431, wherein the valve is an electromagnetic valve, specifically a stop valve 432, the liquid adding pipe is fixed at a preset position at the top of the outer container through a liquid adding pipe clamp 433, and the end part of the liquid adding pipe is a liquid adding port 434.
When the temperature detected by the temperature sensor is not set, the liquid nitrogen replenishing system conveys liquid nitrogen into the liquid nitrogen storage tank through a self-pressurization principle; when the temperature detected by the temperature sensor reaches a set value, the electromagnetic valve is closed.
The Stirling machine set comprises a cold head 31, a heat dissipation supporting plate 32, a Stirling machine 33, a heat dissipation fan 34, a radiator 35, a knife edge flange 36 and a shock absorber 37, wherein the cold head is connected with the Stirling machine, the bottom end of the cold head is assembled with a liquid nitrogen tank body through the knife edge flange, an oxygen-free copper gasket 38 used for sealing is arranged between the cold head and the liquid nitrogen tank body, the heat dissipation supporting plate is arranged on the space where the Stirling machine is located, the heat dissipation fan and the radiator are arranged on the heat dissipation supporting plate in a preset number and a preset position, an axial flow fan 34 is arranged on the left side and the right side for air cooling for cooling heat brought by piston movement of the Stirling machine, and the radiator 35 is specifically a fin radiator (piece 35), wherein a heat exchange surface is added for the Stirling machine to dissipate heat more quickly so as to maintain the upper temperature of the Stirling machine in a reasonable range. The radiator consists of a clamping copper block, a heat pipe and aluminum fins. Before the clamping copper block is matched with the hot-end copper block of the low-temperature refrigerator, a layer of heat conducting paste is uniformly coated on the inner circular surface of the clamping copper block so as to reduce heat conducting resistance between the clamping copper block and the copper block, and the clamping copper block is locked by four locking screws.
The vibration absorber is arranged between the two knife edge flanges, is an expansion joint type corrugated pipe vibration absorber, and is connected with the tank body and the Stirling machine, when the Stirling refrigerator works, the compressor causes certain vibration, the vibration amplitude is transmitted to the vibration absorber, the corrugated pipe on the vibration absorber is elastically deformed, and vibration caused by the Stirling machine is counteracted, so that biological samples stored in the tank are protected from being influenced by Stirling vibration. The two butt flanges are fastened through the bolts, the flat pads, the elastic pads and the nuts, and meanwhile the knife edge surface at the flange enters the oxygen-free copper gasket to form a sealing surface, so that a sealing space of the Stirling liquid nitrogen tank is finally formed.
As an alternative implementation manner, the type TC4187 of the stirling refrigerator used in this embodiment is a pulse tube refrigerator with two opposite motors, and the refrigerator has no other moving parts except the motor piston, and has the advantages of fewer moving parts and low mechanical failure rate compared with the traditional stirling refrigerator, and the opposite motors vibrate to cancel each other out, so that the refrigerator has the advantage of small vibration. The cryocooler consists of the following parts: cold finger, two opposed linear compressors, air reservoir and other parts. Wherein, two opposed linear compressors all adopt the air supporting to support, and when piston and cylinder do relative rectilinear motion, both do not direct contact, and the air film between the two plays the air supporting effect as gas bearing, and this design can effectively prolong the life-span of product. Adequate piston displacement is critical to ensuring air floatation, while the condition for determining piston displacement is the input voltage of the refrigerator, so for a TC4187 low-temperature refrigerator, long-term operation under lower than the minimum voltage can seriously affect the service life of a product, and enough operation voltage is an important factor for ensuring the service life of the product.
Therefore, the present invention requires strict control of the input voltage, so CT5505 is selected as the controller. The controller can provide stable 24V power input for the TC4187 Stirling refrigerator, and in a temperature control mode, the input power of the TC4187 Stirling refrigerator is automatically adjusted after the monitored cold head temperature is compared with the target temperature; and meanwhile, the temperature of the hot end is monitored, and when the monitored temperature exceeds the range, an alarm is automatically given or the machine is automatically turned off, so that the refrigerator is prevented from being damaged by irrecoverable.
The outer surface of the inner vessel is provided with a thermally insulating material 25.
The pressure sensor assembly 42 comprises the pressure sensor, a pressure detection port 421 where the pressure sensor is located is connected with a safety valve 424 communicated with the outside, when the pressure of the liquid nitrogen storage tank is too high, the safety valve is automatically opened, the tank body is protected, when the pressure is relieved to a safety value, the safety valve is automatically closed, and the safety valve is installed at a preset position which is convenient to fix through a pipe clamp 422.
The liquid adding pipe of the liquid nitrogen replenishing system is also connected with a manual liquid adding port 43.
Before use, vacuum pumping is needed between the inner container and the outer container, a liquid adding pipe of the liquid nitrogen replenishing system is arranged in the area, and a vacuum plug 22 is arranged on the outer container. Specifically, in the installation process, the inner container is placed in the outer container lower end socket assembly, and the outer container cylinder 24, the vacuum plug 22, the outer container upper end socket 23, the temperature sensor assembly 41, the pressure sensor assembly 42 and the manual liquid adding port 43 are respectively assembled and welded, and connected with the helium mass spectrometer leak detector, and helium leak detection is carried out on the interlayer, so that the leak rate is required to be less than or equal to 1.0 x 10 -9 Pa.m 3 And(s), opening vacuum plugging after leak detection is qualified, and vacuumizing the tank body through a vacuum unit to 2 x 10 -5 Pa, forming a vacuum insulating interlayer 26, and finally forming a finished tank.
The specific application method of the invention is as follows:
the liquid nitrogen tank is automatically filled into the liquid nitrogen storage tank assembly through a self-pressurization principle, the liquid level is gradually increased, the temperature is gradually reduced, cold energy is transferred into a storage space of the internal swivel through cold and heat exchange of the wall of the storage tank body, the temperature is transferred into the controller through the temperature sensor assembly and displayed, and the stop valve is closed until the temperature reaches a set value.
Because natural heat transfer, liquid nitrogen can volatilize continuously, and 680L nitrogen gas can be volatilized to 1L liquid nitrogen, along with liquid nitrogen sublimates into nitrogen gas, the gas pressure in the liquid nitrogen storage tank will increase, can change the surplus of liquid nitrogen through the atmospheric pressure value to in passing liquid level value to the controller through pressure sensor subassembly, the surplus of liquid nitrogen in the real-time supervision jar.
Nitrogen in the storage tank naturally circulates upwards, and is condensed into liquid nitrogen (Stirling refrigeration technology) when reaching a Stirling cold head, and drops back into the liquid nitrogen storage tank; eventually forming a closed cycle of liquid nitrogen.
When the liquid nitrogen allowance is lower than a set value or the temperature value of the storage space is higher than the set value, the controller controls the Stirling refrigerating unit to start working, and when the liquid nitrogen allowance reaches the set value or the temperature value of the storage space is lower than the set value, the controller controls the Stirling refrigerating unit to stop working.
When the pressure of the liquid nitrogen storage tank is too high, the safety valve (spring fully-opened type) is automatically opened, the tank body is protected, and when the pressure is relieved to a safety value, the safety valve is automatically closed.
When the Stirling engine set fails or the electric energy is insufficient, manual liquid supplementing can be carried out by adopting a manual liquid adding port, and double protection is carried out.
The tank body can be pushed and locked freely by using the casters.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (4)
1. The self-maintaining liquid nitrogen type biological preservation container is characterized by comprising a liquid nitrogen tank body, a Stirling set and a liquid nitrogen replenishment system, wherein the liquid nitrogen tank body comprises a liquid nitrogen storage tank, an inner container sleeved outside the liquid nitrogen storage tank and an outer container sleeved outside the inner container, a material taking port for preserving biological samples is arranged in a space between the inner container and the outer container, the Stirling set is connected to the liquid nitrogen tank body through a flange, a port of the Stirling set is connected with the top end of the liquid nitrogen storage tank, the liquid nitrogen storage tank is connected with the liquid nitrogen replenishment system, a valve is arranged between the liquid nitrogen storage tank and the liquid nitrogen replenishment system, the liquid nitrogen replenishment system is used for supplying a preset amount of liquid nitrogen into the liquid nitrogen storage tank, a pressure sensor is arranged on an exhaust connecting pipe of the storage tank, which is connected with the liquid nitrogen storage tank, a temperature sensor is arranged on the inner container, the pressure sensor and the temperature sensor are connected with a controller, and the controller is connected with the valve, and is used for controlling the opening and closing of the valve and the liquid nitrogen collection set after judging that the surplus reaches a set value or the temperature value of the Stirling storage space is in a set value relation of the set value;
the inner container comprises an inner container barrel, an inner container lower end socket, an inner container upper end socket, a storage tank lower support piece, an inner rotator and an inner rotator rotating piece, wherein the lower part of the inner container barrel is connected with the inner container lower end socket, the upper part of the inner container barrel is connected with the inner container upper end socket, an inner lower end socket inner rotator support piece for installing the inner rotator is arranged on the inner container lower end socket, a storage tank lower support piece for fixing a liquid nitrogen storage tank is arranged on the inner lower end socket inner rotator support piece, the inner rotator comprises an inner rotator inner cylinder and an inner rotator outer cylinder, the inner rotator inner cylinder is connected to the outside of the liquid nitrogen storage tank through the inner rotator upper rotating piece and the inner rotator lower rotating piece, the inner rotator can be manually rotated by taking a sleeve as a center, a plurality of inner rotator partition boards divide a region between the inner rotator inner cylinder and the inner rotator outer cylinder into a plurality of regions, region identifiers are made on the partition boards, and biological samples can be stored in each region independently; when the warehouse-in and warehouse-out operation is performed, the object taking cover is opened, a hand stretches into the internal swivel body to stir the partition plate until a storage area is found, the warehouse-in and warehouse-out operation is performed, after the operation is finished, the cover plate is covered,
the specific using method is as follows:
the liquid nitrogen tank is automatically filled into the liquid nitrogen storage tank assembly through a self-pressurization principle, the liquid level is gradually increased, the temperature is gradually reduced, cold energy is transferred into a storage space of the internal swivel through cold-heat exchange of the cylinder wall of the storage tank, the temperature is transferred into the controller through the temperature sensor assembly and displayed, and the stop valve is closed until the temperature reaches a set value;
the residual quantity of liquid nitrogen can be converted through the air pressure value, the liquid level value is transmitted to the controller through the pressure sensor assembly, and the residual quantity of liquid nitrogen in the tank is monitored in real time;
nitrogen in the storage tank naturally circulates upwards, and is condensed into liquid nitrogen when reaching the Stirling cold head, and drops back into the liquid nitrogen storage tank; finally forming a closed cycle of liquid nitrogen;
when the liquid nitrogen allowance is lower than a set value or the temperature value of the storage space is higher than the set value, the controller controls the Stirling refrigerating unit to start working, and when the liquid nitrogen allowance reaches the set value or the temperature value of the storage space is lower than the set value, the controller controls the Stirling refrigerating unit to stop working;
when the pressure of the liquid nitrogen storage tank is too high, the safety valve is automatically opened to protect the tank body, and when the pressure is relieved to a safety value, the safety valve is automatically closed;
when the Stirling engine set fails or has insufficient electric energy, a manual liquid filling port is adopted for manual liquid filling;
the outer container comprises an outer container cylinder body, an outer container lower end socket, an outer container upper end socket, casters and an inner and outer container support, wherein the lower part of the outer container cylinder body is connected with the outer container lower end socket, the upper part of the outer container cylinder body is connected with the outer container upper end socket, the casters are connected with the lower part of the outer container lower end socket, and the inner and outer container support for fixing the inner container is arranged on the outer container lower end socket;
the inner container support and the outer container support comprise an outer container lower end socket support piece and reinforcing rib plates connected with the outer container lower end socket support piece, the upper end of the outer container lower end socket support piece is connected with the inner lower end socket support piece, and a glass fiber reinforced plastic heat insulation layer is arranged between the upper end of the outer container lower end socket support piece and the inner lower end socket support piece;
the valve is an electromagnetic valve, and when the temperature detected by the temperature sensor is not set, the liquid nitrogen replenishing system conveys liquid nitrogen into the liquid nitrogen storage tank through a self-pressurization principle; when the temperature detected by the temperature sensor reaches a set value, the electromagnetic valve is closed;
the Stirling machine set comprises a cold head, a heat dissipation supporting plate, a Stirling machine, a heat dissipation fan, a radiator, a knife edge flange and a shock absorber, wherein the cold head is connected with the Stirling machine, the bottom end of the cold head is assembled with a liquid nitrogen tank body through the knife edge flange, an oxygen-free copper gasket for sealing is arranged between the cold head and the liquid nitrogen tank body, the heat dissipation supporting plate is arranged on the space where the Stirling machine is located, the heat dissipation fan and the radiator are arranged on the heat dissipation supporting plate in preset quantity and preset positions, and the shock absorber is arranged between the two knife edge flanges;
the pressure detection port where the pressure sensor is located is connected with a safety valve communicated with the outside, when the pressure of the liquid nitrogen storage tank is too high, the safety valve is automatically opened, the tank body is protected, and when the pressure is relieved to a safety value, the safety valve is automatically closed.
2. The self-sustaining liquid nitrogen type biological preservation container according to claim 1, wherein a vacuum pumping treatment is performed between the inner container and the outer container, a vacuum interlayer is arranged between the inner container and the outer container, a liquid adding pipe of the liquid nitrogen replenishing system is arranged in the area, and a vacuum plug is arranged on the outer container.
3. The self-sustaining liquid nitrogen biological storage container of claim 1, wherein the outer surface of said inner container is provided with a thermally insulating material.
4. The self-sustaining liquid nitrogen biological storage container of claim 1, wherein the liquid nitrogen replenishment system is further connected to a manual liquid filling port.
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