CN114011873A - Bioremediation method for petroleum-polluted soil - Google Patents
Bioremediation method for petroleum-polluted soil Download PDFInfo
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- CN114011873A CN114011873A CN202111386045.3A CN202111386045A CN114011873A CN 114011873 A CN114011873 A CN 114011873A CN 202111386045 A CN202111386045 A CN 202111386045A CN 114011873 A CN114011873 A CN 114011873A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a bioremediation method of petroleum polluted soil, which is characterized in that soil conditioning is carried out before maintenance, so that remediation bacteria are uniformly distributed in the soil, meanwhile, the initial contact area of a soil improvement agent and the soil can be greatly increased by ridging the soil, the soil improvement agent can be more uniformly infiltrated into the soil to be remedied, a microorganism conditioner in the soil improvement agent provides a better living environment for the remediation bacteria, a nutrient provides additional nutrition for the remediation bacteria, so that the remediation bacteria can be rapidly bred and remedied, when the soil is maintained, multiple rotary tillage aeration is needed to increase the contact of the remediation bacteria and air, meanwhile, water is sprayed to ensure a certain humidity, so that the remediation bacteria have better oxygen and temperature environments, and simultaneously, microorganism activity detection, soil nutrient component detection and soil oil content detection are carried out in time, finally, grass seeds for greening are scattered, the whole repairing method is relatively simple, and meanwhile, the soil repairing efficiency is better, and the practicability is good.
Description
Technical Field
The invention relates to the technical field of soil remediation, in particular to a bioremediation method of petroleum-polluted soil.
Background
In the processes of petroleum production, storage and transportation, refining and processing and use, overflow and discharge of petroleum hydrocarbons can occur due to accidents, abnormal operation, maintenance and the like. The pollution of petroleum to the environment not only comprises marine pollution, but also comprises a large amount of soil and sandy beach petroleum pollution, the soil polluted by petroleum can be called oil polluted soil and oil sludge, wherein the treatment of the oil polluted soil and the oil sludge comprises thermochemical washing, water washing, incineration and solvent extraction, wherein the thermochemical washing, the water washing, the incineration and the solvent extraction have large additional pollution and extra energy consumption, so the method is low in efficiency for treating the oil polluted soil and the oil sludge.
Today, biological methods are used to remediate petroleum pollution in countries around the world. The biological treatment technology for petroleum pollution is to utilize hydrocarbon substances in petroleum as carbon source and energy source to grow by means of a great amount of microbes, and to degrade the petroleum pollutants in soil into carbon dioxide and water or convert the petroleum pollutants into other harmless substances through the metabolism of the microbes. The main action process of the microorganism on the petroleum pollutants has three aspects: one is the direct absorption of hydrocarbons: certain hydrocarbons in petroleum are capable of entering membranes by direct or indirect dissolution in the lipophilic regions of the membrane; second, biodegradation of alkanes and cycloalkanes; and thirdly, biodegradation of aromatic hydrocarbon, olefin and alkyne. However, the existing biological treatment method for oil-contaminated soil and oil sludge is complicated, and if a large amount of operations are carried out, the problems of low soil remediation efficiency and low practicability exist.
Disclosure of Invention
The invention aims to provide a bioremediation method for petroleum-polluted soil, which has the advantages of relative simplicity, better soil remediation efficiency and good practicability and solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a bioremediation method of petroleum-contaminated soil comprises the following steps:
s1: firstly, adjusting soil conditions, wherein soil polluted by petroleum is collected firstly, preliminarily sorted, cleaned of stones and impurities in the polluted soil and properly crushed;
s2: then tedding and drying, wherein the crushed polluted soil is fully tedded, so that the water content of the polluted soil is reduced, and the bacteria remained in the soil are killed;
s3: preparing a mixed solution of the degrading elements, wherein the mixed solution of the degrading elements is a mixture of the degrading elements, the biological enzyme and the purified water, spraying the mixed solution of the degrading elements on dry polluted soil, stirring the soil while spraying, not spraying when the water content in the polluted soil reaches 40%, and simultaneously carrying out three-day soil conditioning;
s4: adding a soil improvement agent, wherein the soil improvement agent is applied to the surface of the conditioned polluted soil, the soil is uniformly stirred while the soil improvement agent is added, and the soil is divided into a plurality of ridges with the same spacing value;
s5: then, maintaining, namely maintaining the contaminated soil pile added with the soil improvement agent for four weeks, wherein the maintaining comprises rotary tillage aeration, water spraying and moisture retention, microbial activity detection and soil nutrient component detection;
s6: then, carrying out oil content detection on the polluted soil;
s7: finally, grass seeds are sown on the restored soil, and the greening effect is observed.
Further, when the soil condition is adjusted in S1, not only stones and bricks but also plastics visible in the soil need to be removed.
Further, the time for tedding the polluted soil in the step S2 is controlled to be 2-3 days, and the polluted soil is smashed and air-dried in time after the tedding is finished, wherein the air-drying time is 1 day.
Further, should set up the canopy in the in-process of carrying out the soil conditioning in S3, avoid the rainwater to corrode and cause soil moisture too big after the canopy installation, the degradation element in the mixed liquid of degradation element simultaneously is 3 with the biological enzyme proportion: 2, degrading the undiscovered degradable plastics in the polluted soil by using the degrading agent in an accelerated manner.
Further, the soil improvement agent in the S4 comprises a microbial conditioner, a nutrient and a blending agent, wherein the ratio of the microbial conditioner to the nutrient to the blending agent is 2: 2: 1.
further, the microbial conditioner is a water-added mixture of inorganic salt and sodium chloride, and the concentration value of the inorganic salt is greater than that of the sodium chloride.
Further, the nutrient is a mixture of edible oil, beef extract peptone, small molecule peptone, glucose and sodium acetate, wherein the beef extract peptone and the small molecule peptone are mixed and crushed firstly, and the crushed mixed powder is mixed with the edible oil, the glucose and the sodium acetate.
Further, in the step S5, the maintenance sequence includes rotary tillage aeration, water spraying and moisture retention, microbial activity detection and soil nutrient composition detection, wherein a soil improvement agent may be additionally added as required after the microbial activity detection and the soil nutrient composition detection are performed.
Furthermore, the sowing and greening in the S7 are carried out after the oil content in the soil is less than 1.5%, and the grass seeds for greening are alfalfa grass seeds.
Compared with the prior art, the invention has the beneficial effects that: the invention provides a bioremediation method of petroleum-polluted soil, which comprises the steps of adjusting soil conditions, tedding and drying, preparing mixed solution of degrading elements, adding soil improvement agents, maintaining, detecting oil content of soil, sowing, observing, greening and the like, wherein soil conditioning is carried out for three days before maintenance, so that remediation bacteria are uniformly distributed in the soil, better reproduction and soil remediation environments are provided, meanwhile, the initial contact area of the soil improvement agents and the soil can be increased to a greater degree by ridging the soil, the soil improvement agents can be more uniformly infiltrated into the soil to be remedied, the soil improvement agents comprise microbial conditioners, nutritional agents and mixing agents, the microbial conditioners provide better environments for the remediation bacteria, the nutritional agents provide additional nutrition for the remediation bacteria, so that the remediation bacteria can be rapidly reproduced and remedied, when the soil is maintained, need carry out many times rotary tillage aeration and increase restoration bacterium and air contact, the while trickle guarantees certain humidity for restoration bacterium has better oxygen and temperature environment, and the while is timely carries out microbial activity detection, soil nutrient composition detection and soil oil content detection, spreads the grass seeds for afforestation at last, and whole restoration method is simple relatively, has the soil remediation efficiency of preferred simultaneously, and the practicality is good.
Drawings
FIG. 1 is an overall flow diagram of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A bioremediation method of petroleum-contaminated soil comprises the following steps:
the first step is as follows: firstly, adjusting soil conditions, wherein soil polluted by petroleum is collected firstly and is subjected to preliminary sorting, stones and impurities in the polluted soil are removed, the polluted soil is appropriately crushed, when the soil conditions are adjusted, the stones and bricks are removed, visible plastics in the soil also need to be removed, and the influence of impurities on subsequent microorganisms can be reduced by removing the impurities from the soil;
the second step is that: then turning over and drying, wherein the crushed polluted soil is fully turned over and dried, the water content of the polluted soil is reduced, meanwhile, the bacteria remained in the soil are killed, the influence of the bacteria reserved in the soil and the bacteria for subsequent repair can be reduced by killing the bacteria remained in the soil, the turning over and drying time of the polluted soil is controlled to be 2-3 days, the polluted soil is timely crushed and dried after the turning over and drying is finished, and the drying time is 1 day;
the third step: preparing degradation element mixed liquid, wherein degradation element mixed liquid is degradation element, biological enzyme and pure water three's mixture, and spray degradation element mixed liquid on dry contaminated soil, stir soil when spraying, water content in contaminated soil no longer sprays after reaching 40%, carry out three days' soil conditioning simultaneously, should build the canopy at the in-process that carries out the soil conditioning, avoid the rainwater to corrode and cause soil humidity too big behind the canopy installation, degradation element and biological enzyme proportion in the degradation element mixed liquid are 3 simultaneously: 2, degrading undiscovered degradable plastics in the polluted soil by using the degrading agent in an accelerated manner, reducing the plastics and improving the soil quality at the same time, wherein a large amount of bacteria for repairing the soil exist in the biological enzyme, and the repairing bacteria carry out reproduction and soil repair in a slightly humid soil environment;
the fourth step: and then adding a soil improvement agent, wherein the soil improvement agent is applied to the surface of the conditioned polluted soil, the soil is uniformly mixed while the soil improvement agent is added, the soil is divided into a plurality of ridges with the same spacing value, the initial contact area between the soil improvement agent and the soil can be increased to a greater extent by dividing the contaminated soil into ridges, the soil improvement agent can be more uniformly infiltrated into the soil to be repaired, the soil improvement agent comprises a microbial conditioner, a nutrient and a mixing agent, and the ratio of the microbial conditioner, the nutrient and the mixing agent is 2: 2: 1, a microbial conditioner is a water-added mixture of inorganic salt and sodium chloride, the concentration value of the inorganic salt is greater than that of the sodium chloride, a nutritional agent is a mixture of edible oil, beef extract peptone, small molecule peptone, glucose and sodium acetate, wherein the beef extract peptone and the small molecule peptone are mixed and crushed firstly, and the crushed mixed powder is mixed with the edible oil, the glucose and the sodium acetate, wherein the microbial conditioner provides a better living environment for repairing bacteria, and the nutritional agent provides additional nutrition for repairing bacteria, so that the repairing bacteria can be quickly reproduced and repaired;
the fifth step: then, maintaining the polluted soil pile added with the soil improvement agent, wherein the maintenance time is four weeks, the maintenance comprises rotary tillage aeration, water spraying moisture retention, microbial activity detection and soil nutrient component detection, the maintenance sequence comprises the rotary tillage aeration, the water spraying moisture retention, the microbial activity detection and the soil nutrient component detection, wherein the soil improvement agent can be additionally added according to the requirement after the microbial activity detection and the soil nutrient component detection are carried out, when the maintenance is carried out, the rotary tillage aeration is carried out for a plurality of times every day, the rotary tillage aeration can effectively increase the contact of the repairing bacteria and the air, meanwhile, the water spraying ensures a certain humidity, so that the repairing bacteria have better oxygen and temperature environments, the reproduction and the soil repair of the repairing bacteria are accelerated, when the repairing bacteria carry out the soil repair, the microbial activity detection and the soil nutrient component detection are carried out in time, when the number of the repairing bacteria is reduced or the activity is reduced, a proper amount of mixed liquor of the soil improvement agent and the degrading agent is supplemented;
and a sixth step: then, carrying out oil content detection on the polluted soil, and often detecting the oil content of the polluted soil so as to facilitate subsequent soil improvement;
the seventh step: and finally, sowing grass seeds on the repaired soil, observing the greening effect, wherein the sowing and greening are carried out after the oil content of the soil is less than 1.5%, the grass seeds for greening adopt alfalfa seeds, wherein the alfalfa seeds are sown in the good soil and covered by thin soil after the soil is improved to a great extent, so that whether the repaired soil can be used for plant planting is observed, and whether the alfalfa can further degrade the polluted soil is examined.
The method for biologically repairing the petroleum polluted soil comprises the steps of adjusting soil conditions, tedding and drying, preparing a mixed solution of degradants, adding a soil improvement agent, maintaining, detecting the oil content of the soil, sowing, observing, greening and the like, wherein the soil conditions are firstly adjusted, stones and sundries in the polluted soil are removed, the stones and sundries are appropriately crushed, then the soil conditions are tedded and dried, the water of the polluted soil is reduced, the bacteria remained in the soil are killed, the influence on the bacteria for subsequent repair is reduced, then the mixed solution of the degradants is prepared, the mixed solution of the degradants is sprayed on the dry polluted soil and is stirred simultaneously, soil conditioning is carried out for three days, the degradants accelerate the degradation of the plastic which is not found in the polluted soil, a large amount of bacteria for repairing the soil exist in biological enzymes, and the repairing bacteria are reproduced and repaired in a micro-moist soil environment, adding a soil improvement agent, ridging the soil, ridging the polluted soil to increase the initial contact area of the soil improvement agent and the soil to a greater extent, wherein the soil improvement agent can be more uniformly infiltrated into the soil to be repaired, the soil improvement agent comprises a microbial conditioner, a nutrient and a mixing agent, the microbial conditioner provides a better living environment for repairing bacteria, the nutrient provides additional nutrition for the repairing bacteria, so that the repairing bacteria can be rapidly reproduced and repaired, then maintaining is carried out, the maintenance comprises rotary tillage aeration, water drenching moisture retention, microbial activity detection and soil nutrient component detection, wherein in the maintenance, multiple times of aeration are needed every day, the rotary tillage aeration can effectively increase the contact between the repairing bacteria and air, and meanwhile, the water drenching ensures certain humidity, so that the repairing bacteria have better oxygen and temperature environments, the method is characterized in that the reproduction of the repairing bacteria and the soil repairing are accelerated, meanwhile, the microbial activity detection, the soil nutrient component detection and the soil oil content detection need to be carried out in time, when the number of the repairing bacteria is reduced or the activity is reduced, a proper amount of mixed liquid of a soil improving agent and a degrading element is supplemented, wherein after the soil is improved to a large extent, alfalfa seeds are sown in the good soil so as to observe whether the repaired soil can be used for plant planting.
In conclusion, the bioremediation method of the petroleum-polluted soil provided by the invention comprises the steps of adjusting soil conditions, tedding and drying, preparing a mixed solution of degrading elements, adding a soil improvement agent, maintaining, detecting oil content in the soil, sowing, observing and greening and the like, wherein soil conditioning is carried out for three days before maintenance, so that the remediation bacteria are uniformly distributed in the soil, better reproduction and soil remediation environments are provided, meanwhile, the initial contact area between the soil improvement agent and the soil can be increased to a greater extent by ridging the soil, the soil improvement agent can be more uniformly infiltrated into the soil to be remedied, the soil improvement agent comprises a microbial conditioner, a nutrient and a mixing agent, wherein the microbial conditioner provides a better living environment for the remediation bacteria, the nutrient provides additional nutrition for the remediation bacteria, so that the remediation bacteria can be rapidly reproduced and remedied, during soil maintenance, rotary tillage aeration is carried out for multiple times to increase contact between remediation bacteria and air, and meanwhile, water spraying guarantees certain humidity, so that the remediation bacteria have better oxygen and temperature environments, microorganism activity detection, soil nutrient component detection and soil oil content detection are carried out timely, and greening grass seeds are finally sown.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (9)
1. The bioremediation method of the petroleum-polluted soil is characterized by comprising the following steps:
s1: firstly, adjusting soil conditions, wherein soil polluted by petroleum is collected firstly, preliminarily sorted, cleaned of stones and impurities in the polluted soil and properly crushed;
s2: then tedding and drying, wherein the crushed polluted soil is fully tedded, so that the water content of the polluted soil is reduced, and the bacteria remained in the soil are killed;
s3: preparing a mixed solution of the degrading elements, wherein the mixed solution of the degrading elements is a mixture of the degrading elements, the biological enzyme and the purified water, spraying the mixed solution of the degrading elements on dry polluted soil, stirring the soil while spraying, not spraying when the water content in the polluted soil reaches 40%, and simultaneously carrying out three-day soil conditioning;
s4: adding a soil improvement agent, wherein the soil improvement agent is applied to the surface of the conditioned polluted soil, the soil is uniformly stirred while the soil improvement agent is added, and the soil is divided into a plurality of ridges with the same spacing value;
s5: then, maintaining, namely maintaining the contaminated soil pile added with the soil improvement agent for four weeks, wherein the maintaining comprises rotary tillage aeration, water spraying and moisture retention, microbial activity detection and soil nutrient component detection;
s6: then, carrying out oil content detection on the polluted soil;
s7: finally, grass seeds are sown on the restored soil, and the greening effect is observed.
2. The bioremediation method for soil contaminated with petroleum according to claim 1, wherein the soil conditioning in S1 is performed to remove not only rocks and bricks but also plastics visible in the soil.
3. The bioremediation method of petroleum-contaminated soil according to claim 1, wherein the time for tedding the contaminated soil in S2 is controlled to be 2-3 days, and the soil is immediately smashed and air-dried after the tedding is finished, wherein the air-drying time is 1 day.
4. The bioremediation method of petroleum-contaminated soil according to claim 1, wherein a rain shelter is constructed in the process of soil conditioning in S3, the rain shelter is installed to prevent excessive soil humidity caused by rain erosion, and the ratio of the degradation elements to the biological enzymes in the mixed solution of the degradation elements is 3: 2, degrading the undiscovered degradable plastics in the polluted soil by using the degrading agent in an accelerated manner.
5. The bioremediation method for soil contaminated by petroleum according to claim 1, wherein the soil improvement agent in S4 comprises a microbial conditioner, a nutrient and a blending agent, and the ratio of the microbial conditioner, the nutrient and the blending agent is 2: 2: 1.
6. the bioremediation method of soil contaminated by petroleum according to claim 5, wherein the microbial conditioner is a water-added mixture of inorganic salts and sodium chloride, and the concentration value of the inorganic salts is greater than that of the sodium chloride.
7. The bioremediation method for petroleum-contaminated soil according to claim 5, wherein the nutrient is a mixture of edible oil, beef extract peptone, small molecule peptone, glucose and sodium acetate, wherein the beef extract peptone and the small molecule peptone are first mixed and pulverized, and the pulverized mixed powder is mixed with the edible oil, the glucose and the sodium acetate.
8. The bioremediation method of soil contaminated by petroleum according to claim 1, wherein the maintenance sequence of S5 includes rotary tillage aeration, water spraying and moisture retention, microbial activity detection and soil nutrient detection, and soil improvement agents can be additionally added after the microbial activity detection and the soil nutrient detection according to the requirement.
9. The bioremediation method for petroleum-contaminated soil according to claim 1, wherein the planting and greening in S7 are performed after the oil content in the soil is less than 1.5%, and the grass seeds for greening are alfalfa seeds.
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| CN202111386045.3A CN114011873A (en) | 2021-11-22 | 2021-11-22 | Bioremediation method for petroleum-polluted soil |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117066261A (en) * | 2023-09-11 | 2023-11-17 | 中国科学院西北生态环境资源研究院 | Petroleum contaminated soil regeneration and restoration device and restoration method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103480644A (en) * | 2013-10-12 | 2014-01-01 | 南开大学 | Plant-microorganism combined method for enhanced repairing of petroleum-contaminated spetroleum |
| CN103627658A (en) * | 2013-11-27 | 2014-03-12 | 安徽远大机械制造有限公司 | Microbial remediation agent for remediating soil ecosystem |
| CN106001104A (en) * | 2016-08-08 | 2016-10-12 | 西南石油大学 | Method for restoration of petroleum-polluted soil by aboriginal bacteria |
| CN107971333A (en) * | 2016-10-21 | 2018-05-01 | 西南石油大学 | A kind of antimicrobial plant joint repairs the fast method of the high soil containing greasy dirt |
| CN109108063A (en) * | 2018-10-29 | 2019-01-01 | 中国石油大学(华东) | A kind of biology expedient of tillage oily sludge soil remediation construction technology |
| CN112251373A (en) * | 2020-10-12 | 2021-01-22 | 中国科学院沈阳应用生态研究所 | Preparation and application of bacterium-enzyme composite preparation for petroleum hydrocarbon degradation |
| CN113214033A (en) * | 2021-04-19 | 2021-08-06 | 东北大学 | Degradation treatment method of urban sludge micro-plastic |
-
2021
- 2021-11-22 CN CN202111386045.3A patent/CN114011873A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103480644A (en) * | 2013-10-12 | 2014-01-01 | 南开大学 | Plant-microorganism combined method for enhanced repairing of petroleum-contaminated spetroleum |
| CN103627658A (en) * | 2013-11-27 | 2014-03-12 | 安徽远大机械制造有限公司 | Microbial remediation agent for remediating soil ecosystem |
| CN106001104A (en) * | 2016-08-08 | 2016-10-12 | 西南石油大学 | Method for restoration of petroleum-polluted soil by aboriginal bacteria |
| CN107971333A (en) * | 2016-10-21 | 2018-05-01 | 西南石油大学 | A kind of antimicrobial plant joint repairs the fast method of the high soil containing greasy dirt |
| CN109108063A (en) * | 2018-10-29 | 2019-01-01 | 中国石油大学(华东) | A kind of biology expedient of tillage oily sludge soil remediation construction technology |
| CN112251373A (en) * | 2020-10-12 | 2021-01-22 | 中国科学院沈阳应用生态研究所 | Preparation and application of bacterium-enzyme composite preparation for petroleum hydrocarbon degradation |
| CN113214033A (en) * | 2021-04-19 | 2021-08-06 | 东北大学 | Degradation treatment method of urban sludge micro-plastic |
Non-Patent Citations (2)
| Title |
|---|
| 杨玉红: "《食品微生物检验技术》", 31 January 2011, 中国计量出版社 * |
| 黄占斌: "《环境科学》", 31 January 2017, 冶金工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117066261A (en) * | 2023-09-11 | 2023-11-17 | 中国科学院西北生态环境资源研究院 | Petroleum contaminated soil regeneration and restoration device and restoration method |
| CN117066261B (en) * | 2023-09-11 | 2024-03-15 | 中国科学院西北生态环境资源研究院 | Petroleum contaminated soil regeneration and restoration device and restoration method |
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