CN114009780A - Food for repairing nerve cells and preparation method thereof - Google Patents
Food for repairing nerve cells and preparation method thereof Download PDFInfo
- Publication number
- CN114009780A CN114009780A CN202111361830.3A CN202111361830A CN114009780A CN 114009780 A CN114009780 A CN 114009780A CN 202111361830 A CN202111361830 A CN 202111361830A CN 114009780 A CN114009780 A CN 114009780A
- Authority
- CN
- China
- Prior art keywords
- extract
- birch
- nerve cells
- food
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000002569 neuron Anatomy 0.000 title claims abstract description 53
- 235000013305 food Nutrition 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 235000018185 Betula X alpestris Nutrition 0.000 claims abstract description 100
- 235000018212 Betula X uliginosa Nutrition 0.000 claims abstract description 100
- 239000000284 extract Substances 0.000 claims abstract description 87
- 239000000843 powder Substances 0.000 claims abstract description 50
- 238000002156 mixing Methods 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 22
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000008213 purified water Substances 0.000 claims abstract description 15
- 238000007873 sieving Methods 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims description 39
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 27
- 239000012530 fluid Substances 0.000 claims description 22
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 18
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 16
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 239000000287 crude extract Substances 0.000 claims description 13
- 239000000706 filtrate Substances 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 8
- 239000001569 carbon dioxide Substances 0.000 claims description 8
- 238000009210 therapy by ultrasound Methods 0.000 claims description 8
- 239000001294 propane Substances 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000010992 reflux Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
- 239000008518 lycium barbarum polysaccharide Substances 0.000 claims description 4
- 235000015193 tomato juice Nutrition 0.000 claims description 4
- 240000000851 Vaccinium corymbosum Species 0.000 claims description 3
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims description 3
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims description 3
- 235000021014 blueberries Nutrition 0.000 claims description 3
- 235000019674 grape juice Nutrition 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 2
- 230000008439 repair process Effects 0.000 abstract description 12
- 230000036737 immune function Effects 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000035764 nutrition Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 230000036541 health Effects 0.000 abstract description 2
- FVWJYYTZTCVBKE-ROUWMTJPSA-N betulin Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(CO)CC[C@@H](C(=C)C)[C@@H]5[C@H]4CC[C@@H]3[C@]21C FVWJYYTZTCVBKE-ROUWMTJPSA-N 0.000 description 27
- 230000000694 effects Effects 0.000 description 27
- JYDNKGUBLIKNAM-UHFFFAOYSA-N Oxyallobutulin Natural products C1CC(=O)C(C)(C)C2CCC3(C)C4(C)CCC5(CO)CCC(C(=C)C)C5C4CCC3C21C JYDNKGUBLIKNAM-UHFFFAOYSA-N 0.000 description 26
- MVIRREHRVZLANQ-UHFFFAOYSA-N betulin Natural products CC(=O)OC1CCC2(C)C(CCC3(C)C2CC=C4C5C(CCC5(CO)CCC34C)C(=C)C)C1(C)C MVIRREHRVZLANQ-UHFFFAOYSA-N 0.000 description 26
- 238000000926 separation method Methods 0.000 description 14
- QGJZLNKBHJESQX-UHFFFAOYSA-N 3-Epi-Betulin-Saeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C(=C)C)C5C4CCC3C21C QGJZLNKBHJESQX-UHFFFAOYSA-N 0.000 description 13
- CLOUCVRNYSHRCF-UHFFFAOYSA-N 3beta-Hydroxy-20(29)-Lupen-3,27-oic acid Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C(O)=O)CCC5(C)CCC(C(=C)C)C5C4CCC3C21C CLOUCVRNYSHRCF-UHFFFAOYSA-N 0.000 description 13
- DIZWSDNSTNAYHK-XGWVBXMLSA-N Betulinic acid Natural products CC(=C)[C@@H]1C[C@H]([C@H]2CC[C@]3(C)[C@H](CC[C@@H]4[C@@]5(C)CC[C@H](O)C(C)(C)[C@@H]5CC[C@@]34C)[C@@H]12)C(=O)O DIZWSDNSTNAYHK-XGWVBXMLSA-N 0.000 description 13
- PZXJOHSZQAEJFE-UHFFFAOYSA-N dihydrobetulinic acid Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C(C)C)C5C4CCC3C21C PZXJOHSZQAEJFE-UHFFFAOYSA-N 0.000 description 13
- MQYXUWHLBZFQQO-UHFFFAOYSA-N nepehinol Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C)CCC(C(=C)C)C5C4CCC3C21C MQYXUWHLBZFQQO-UHFFFAOYSA-N 0.000 description 13
- QGJZLNKBHJESQX-FZFNOLFKSA-N betulinic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C(=C)C)[C@@H]5[C@H]4CC[C@@H]3[C@]21C QGJZLNKBHJESQX-FZFNOLFKSA-N 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 7
- 210000005036 nerve Anatomy 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 230000006870 function Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 230000003110 anti-inflammatory effect Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000006378 damage Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 210000004556 brain Anatomy 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 210000001320 hippocampus Anatomy 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 210000000653 nervous system Anatomy 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 238000001953 recrystallisation Methods 0.000 description 3
- 208000024827 Alzheimer disease Diseases 0.000 description 2
- 208000018737 Parkinson disease Diseases 0.000 description 2
- 230000000949 anxiolytic effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 208000026106 cerebrovascular disease Diseases 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 210000004295 hippocampal neuron Anatomy 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000002633 protecting effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 235000018553 tannin Nutrition 0.000 description 2
- 229920001864 tannin Polymers 0.000 description 2
- 239000001648 tannin Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 238000010175 APPswe/PSEN1dE9 Methods 0.000 description 1
- 208000019901 Anxiety disease Diseases 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 206010008111 Cerebral haemorrhage Diseases 0.000 description 1
- 208000010693 Charcot-Marie-Tooth Disease Diseases 0.000 description 1
- 201000008992 Charcot-Marie-Tooth disease type 1B Diseases 0.000 description 1
- 206010008748 Chorea Diseases 0.000 description 1
- 208000027205 Congenital disease Diseases 0.000 description 1
- 206010010506 Congenital hydrocephalus Diseases 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 206010012289 Dementia Diseases 0.000 description 1
- 208000016192 Demyelinating disease Diseases 0.000 description 1
- 108700019745 Disks Large Homolog 4 Proteins 0.000 description 1
- 102000047174 Disks Large Homolog 4 Human genes 0.000 description 1
- 208000027776 Extrapyramidal disease Diseases 0.000 description 1
- 206010063006 Facial spasm Diseases 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- 108090000079 Glucocorticoid Receptors Proteins 0.000 description 1
- 102100033417 Glucocorticoid receptor Human genes 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 235000018142 Hedysarum alpinum var americanum Nutrition 0.000 description 1
- 240000006461 Hedysarum alpinum var. americanum Species 0.000 description 1
- 208000002972 Hepatolenticular Degeneration Diseases 0.000 description 1
- 208000004356 Hysteria Diseases 0.000 description 1
- 230000004950 I-kappaB phosphorylation Effects 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 208000026072 Motor neurone disease Diseases 0.000 description 1
- 208000001089 Multiple system atrophy Diseases 0.000 description 1
- 208000003926 Myelitis Diseases 0.000 description 1
- 102000003945 NF-kappa B Human genes 0.000 description 1
- 108010057466 NF-kappa B Proteins 0.000 description 1
- 208000028389 Nerve injury Diseases 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- 208000025966 Neurological disease Diseases 0.000 description 1
- 206010029333 Neurosis Diseases 0.000 description 1
- 102000008299 Nitric Oxide Synthase Human genes 0.000 description 1
- 108010021487 Nitric Oxide Synthase Proteins 0.000 description 1
- 208000021384 Obsessive-Compulsive disease Diseases 0.000 description 1
- 206010068106 Occipital neuralgia Diseases 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 102100026918 Phospholipase A2 Human genes 0.000 description 1
- 101710096328 Phospholipase A2 Proteins 0.000 description 1
- 208000028872 Progressive muscular dystrophy Diseases 0.000 description 1
- 102000003923 Protein Kinase C Human genes 0.000 description 1
- 108090000315 Protein Kinase C Proteins 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 208000008765 Sciatica Diseases 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- 208000029033 Spinal Cord disease Diseases 0.000 description 1
- 206010041549 Spinal cord compression Diseases 0.000 description 1
- 206010041591 Spinal osteoarthritis Diseases 0.000 description 1
- 208000009415 Spinocerebellar Ataxias Diseases 0.000 description 1
- 208000010112 Spinocerebellar Degenerations Diseases 0.000 description 1
- 102000009822 Sterol Regulatory Element Binding Proteins Human genes 0.000 description 1
- 108010020396 Sterol Regulatory Element Binding Proteins Proteins 0.000 description 1
- 208000032851 Subarachnoid Hemorrhage Diseases 0.000 description 1
- 102000001435 Synapsin Human genes 0.000 description 1
- 108050009621 Synapsin Proteins 0.000 description 1
- 206010042928 Syringomyelia Diseases 0.000 description 1
- 201000004810 Vascular dementia Diseases 0.000 description 1
- 206010047249 Venous thrombosis Diseases 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000002552 acute disseminated encephalomyelitis Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 201000003352 adrenal gland pheochromocytoma Diseases 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000001430 anti-depressive effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000000935 antidepressant agent Substances 0.000 description 1
- 229940005513 antidepressants Drugs 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 239000002249 anxiolytic agent Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 208000025222 central nervous system infectious disease Diseases 0.000 description 1
- 206010008118 cerebral infarction Diseases 0.000 description 1
- 206010008129 cerebral palsy Diseases 0.000 description 1
- 208000036319 cervical spondylosis Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 208000012601 choreatic disease Diseases 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 208000010877 cognitive disease Diseases 0.000 description 1
- 230000003920 cognitive function Effects 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 208000012839 conversion disease Diseases 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 206010014599 encephalitis Diseases 0.000 description 1
- 210000003038 endothelium Anatomy 0.000 description 1
- 206010015037 epilepsy Diseases 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 208000014337 facial nerve disease Diseases 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 208000035474 group of disease Diseases 0.000 description 1
- 201000003636 hereditary ataxia Diseases 0.000 description 1
- 208000003906 hydrocephalus Diseases 0.000 description 1
- 230000000147 hypnotic effect Effects 0.000 description 1
- 210000001169 hypoglossal nerve Anatomy 0.000 description 1
- 210000003016 hypothalamus Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000037456 inflammatory mechanism Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- NKMDIWKRKQFYPH-VIUFNMEASA-N lupane Chemical compound C1CCC(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C)CC[C@@H](C(C)C)[C@@H]5[C@H]4CC[C@@H]3[C@]21C NKMDIWKRKQFYPH-VIUFNMEASA-N 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 208000005264 motor neuron disease Diseases 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 201000006417 multiple sclerosis Diseases 0.000 description 1
- 206010028417 myasthenia gravis Diseases 0.000 description 1
- 230000008764 nerve damage Effects 0.000 description 1
- 208000004296 neuralgia Diseases 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 230000001272 neurogenic effect Effects 0.000 description 1
- 210000000715 neuromuscular junction Anatomy 0.000 description 1
- 208000002040 neurosyphilis Diseases 0.000 description 1
- 208000015238 neurotic disease Diseases 0.000 description 1
- 238000007034 nitrosation reaction Methods 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000005937 nuclear translocation Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 150000002966 pentacyclic triterpenoids Chemical class 0.000 description 1
- 208000029308 periodic paralysis Diseases 0.000 description 1
- 208000033808 peripheral neuropathy Diseases 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 208000019899 phobic disease Diseases 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 208000005987 polymyositis Diseases 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000000770 proinflammatory effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000011506 response to oxidative stress Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 208000005801 spondylosis Diseases 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 210000000225 synapse Anatomy 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 206010044652 trigeminal neuralgia Diseases 0.000 description 1
- -1 triterpene compound Chemical class 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a food for repairing nerve cells and a preparation method thereof, and relates to the technical field of health care products. A method for preparing a food for repairing nerve cells, comprising: micronizing cortex Betulae Pendulae at 50-60 deg.C, and sieving with 80-90 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 20-30min to obtain extract; separating and purifying the extract to obtain refined birch extract, and mixing the birch extract with purified water according to a mass ratio (0.01-1): 5 mixing, and oscillating for 10-20min at 20-30KHz frequency. The preparation method is simple and easy to operate, and is beneficial to large-scale production. The food prepared by the invention not only can effectively repair nerve cells and improve the function of the nerve cells, but also can provide nutrition for organisms and strengthen the immune function of the organisms, and is non-toxic and harmless.
Description
Technical Field
The invention relates to the technical field of health care products, in particular to a food for repairing nerve cells and a preparation method thereof.
Background
Neurological diseases are a large group of diseases, including a large number of diseases, and currently form a complete discipline called neurology.
According to the content, onset form and nature of the disease, the following parts are included: the first is peripheral neuropathy including trigeminal neuralgia, facial neuritis, facial spasm, occipital neuralgia, cervical spondylosis, sciatica, etc. The second category is spinal cord disease, including acute myelitis, spinal cord compression, syringomyelia, and the like. The third category, cerebrovascular diseases, includes cerebral infarction, cerebral hemorrhage, subarachnoid hemorrhage, intracranial venous thrombosis, vascular dementia, etc. The fourth category, central nervous system infectious diseases, includes various types of encephalitis, meningitis, neurosyphilis, parasitosis, etc. The fifth group, extrapyramidal diseases, includes paralysis agitans (Parkinson's disease), hepatolenticular degeneration, chorea minor. The sixth, epilepsy. The seventh category, demyelinating diseases, includes multiple sclerosis, acute disseminated encephalomyelitis, and the like. The eighth group, diseases of the neuro-muscular junction, include myasthenia gravis, progressive muscular dystrophy, periodic paralysis, polymyositis, and the like. The ninth group, degenerative diseases of the nervous system, such as motor neuron disease, Alzheimer's disease, multiple system atrophy, and the like. The tenth group, congenital diseases of the nervous system, including cerebral palsy, congenital hydrocephalus. The eleventh category, hereditary diseases of the nervous system, includes hereditary ataxia, peroneal muscular atrophy. The twelfth group, neurosis, includes hysteria, anxiety, obsessive compulsive disorder, neurasthenia, phobias, hypochondriac disorders, and the like.
The existing medicines can only moderately relieve the nerve pain and can not repair nerve cells.
Disclosure of Invention
The invention aims to provide a food for repairing nerve cells, which not only can effectively repair nerve cells and improve the functions of the nerve cells, but also can provide nutrition for a human body and strengthen the immune function of the human body, and is non-toxic and harmless.
The invention also aims to provide a preparation method of the food for repairing the nerve cells, which is simple and easy to operate and is beneficial to large-scale production.
The technical problem to be solved by the invention is realized by adopting the following technical scheme.
The invention provides a food for repairing nerve cells, which is prepared by the preparation method of the food for repairing nerve cells.
The invention provides a preparation method of a food for repairing nerve cells, which comprises the following steps:
micronizing cortex Betulae Pendulae at 50-60 deg.C, and sieving with 80-90 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 20-30min to obtain extract; separating and purifying the extract to obtain refined birch extract, and mixing the birch extract with purified water according to a mass ratio (0.01-1): 5 mixing, and oscillating for 10-20min at 20-30KHz frequency.
The food for repairing nerve cells and the preparation method of the food for repairing nerve cells provided by the embodiment of the invention have at least the following beneficial effects:
the action mechanism of the food is as follows: nerves of various organs of the human body are directed by the brain nervous system center and are the most precise and complex organs. After the nerve cells are damaged, the nerve cells cannot automatically repair the self-healing system of the human body, and a repair signal must be sent to neurons of a command center of the brain, so that the brain nerve cell command center can transmit a self-repair signal to the damaged nerves. The food can effectively break through blood brain barrier, is absorbed by hypoglossal nerve, blood vessel and mucosa, and directly reaches nerve cell command central neuron of hypothalamus, and after receiving the repairing signal, the neuron immediately sends the repairing signal to the nerve of the damaged part, and the damaged nerve immediately starts repairing activity.
In the invention, the birch bark is superfine crushed at 50-60 ℃, the hardness of the birch bark is reduced, the brittleness is improved, the crushing is more convenient, the crushing efficiency and the crushing effect are provided, the uniformity of the obtained powder is higher, the amount of the filtered powder is less during sieving, so that more birch powder is obtained, the amount of effective components extracted from the birch powder is further improved, and the temperature does not influence the effective components of the birch bark. The birch powder is obtained by sieving with a sieve of 80-90 meshes, under the particle size, the effective components in the birch powder can be extracted more easily, and the effective components can be extracted more thoroughly, so that the extraction efficiency can be improved, and the extraction rate of the effective components can also be improved. Performing supercritical extraction on birch powder for 20-30min, wherein the supercritical extraction can effectively prevent oxidation and dissipation of thermosensitive substances and maintain effective components of the raw materials; and the extraction and the separation are combined into one, so that the supercritical fluid and the extracted effective components do not need to be separated again. The effective components in the birch powder can be completely extracted by extracting in the time. The extract is separated and purified, so that the purity and concentration of the effective components can be improved, the impurity content is reduced, and the effect is more easily realized. Mixing birch extract and purified water at a mass ratio of (0.01-1): 5 mixing, and oscillating for 10-20min at 20-30KHz frequency. The birch extract is mixed with purified water according to a proportion, so that the damage to a human body caused by too high concentration of the birch extract is avoided, and the repairing effect of the food on nerve cells is best under the proportion. By oscillating and mixing, the uniformity of the finished product can be improved, the dilution effect of the birch extract is better, and the obtained finished product has the best dilution effect and the best repairing effect on nerve cells by oscillating under the conditions.
According to the invention, the food can effectively repair nerve cells, improve the function of the nerve cells, provide organism nutrition, strengthen the immune function of the organism, better repair the nerve cells and is non-toxic and harmless.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.
A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 50-60 deg.C, and sieving with 80-90 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 20-30min to obtain extract; separating and purifying the extract to obtain refined birch extract, and mixing the birch extract with purified water according to a mass ratio (0.01-1): 5 mixing, and oscillating for 10-20min at 20-30KHz frequency.
The cortex Betulae Pendulae contains betulin (chemical component) about 35%, various higher fatty acids above 35%, tannin about 7% (endothelium contains tannin about 11%), phenolic substance, reducing substance, polysaccharides, saponin, protein, triterpenes, total glycosides, resin and oil 12%.
In this example, the main components of the birch extract are betulin and betulinic acid. Wherein, the betulin has mild anti-inflammatory effect, and the mechanism of the anti-inflammatory effect is inhibition of non-nerve genes; betulin can also inhibit the maturation of sterol regulatory element binding protein (SBERP), thereby reducing the biosynthesis of cholesterol and fatty acids; betulin also has antiviral, antidepressant, sedative, hypnotic, and free radical scavenging effects. The betulin can also protect hippocampal neurons, specifically, plays a role in protecting synapses by increasing the expression of PSD-95 and Synapsin-I of hippocampus, reduces the damage of oxidative stress to hippocampal neurons, plays a role in improving the learning and memory capacity of APP/PS1 double-transgene dementia model mice, enhances the long time course of hippocampus, and protects the cognitive function of experimental animals.
Betulinic acid is a natural lupane pentacyclic triterpene compound. Most pentacyclic triterpenoids have anti-inflammatory activity. Betulin and betulinic acid have mild anti-inflammatory property at higher concentration, and their anti-inflammatory property may depend on inhibition of protein kinase C, without involving any neurogenic inflammatory mechanism, specifically, by inhibiting NF-kB signal pathway, inhibiting gene and protein expression of downstream regulated inflammatory mediators; the purposes of diminishing inflammation are achieved by inhibiting the activity of PLA2, inhibiting the activity of nitric oxide synthase, reducing NO generation, promoting ROS to generate oxidative stress reaction and the like; BA is able to inhibit I κ B phosphorylation, p65 phosphorylation and nuclear translocation, as well as transcription of NF- κ B-dependent related genes. It has also been shown that its activity is the result of interaction with the glucocorticoid receptor. Betulinic acid has certain improving effect on senile dementia, and its protecting effect mainly reduces the proinflammatory cytokines in hippocampus and oxidation and nitrosation reactions by weakening the nerve behavior and cognitive dysfunction. Betulinic acid has a certain anxiolytic effect, and a betulinic acid component has been applied to some anxiolytic drugs, which may be related to the higher lipid solubility of betulinic acid, which can permeate brain barrier.
The nerve of the damaged part is repaired by the neuron through the regulating effect of the betulin and the betulinic acid on the nerve cell neuron, the repairing effect is good, the repairing speed is high, and the life quality of a patient with nerve damage can be obviously improved.
The birch bark is superfine pulverized at 50-60 ℃, the hardness of the birch bark is reduced, the brittleness of the birch bark is improved, the birch bark is more convenient to pulverize, the pulverizing efficiency and the pulverizing effect are provided, the obtained powder is higher in uniformity, the amount of the filtered powder is less during sieving, the birch powder is more, the amount of effective components extracted from the birch powder is further increased, and the temperature cannot influence the effective components of the birch bark. The birch powder is obtained by sieving with a sieve of 80-90 meshes, under the particle size, the effective components in the birch powder can be extracted more easily, and the effective components can be extracted more thoroughly, so that the extraction efficiency can be improved, and the extraction rate of the effective components can also be improved.
Performing supercritical extraction on birch powder for 20-30min, wherein the supercritical extraction can effectively prevent oxidation and dissipation of thermosensitive substances and maintain effective components of the raw materials; and the extraction and the separation are combined into one, so that the supercritical fluid and the extracted effective components do not need to be separated again. The effective components in the birch powder can be completely extracted by extracting at the time. The extract is separated and purified, so that the purity and concentration of the effective components can be improved, the impurity content is reduced, and the effect is more easily realized. Mixing birch extract and purified water at a mass ratio of (0.01-1): 5 mixing, and oscillating for 10-20min at 20-30KHz frequency. The birch extract is mixed with purified water according to a proportion, so that the damage to a human body caused by too high concentration of the birch extract is avoided, and the repairing effect of the food on nerve cells is best under the proportion. By oscillating and mixing, the uniformity of the finished product can be improved, the dilution effect of the birch extract is better, and the obtained finished product has the best dilution effect and the best repairing effect on nerve cells by oscillating under the conditions.
During supercritical extraction, the flow rate of the supercritical fluid is 12-18kg/h, and the supercritical fluid is carbon dioxide and/or propane. During extraction, the extraction temperature is 45-55 ℃, and the extraction pressure is 22-30 MPa. The extraction method also comprises ultrasonic treatment for 4-8min, and treatment every 5-10 min.
The flow rate of the supercritical fluid is 12-18 kg/h. The supercritical fluid has the characteristics of both liquid and gas, has the density close to that of the liquid, the viscosity close to that of the gas and a high diffusion coefficient, and therefore has strong dissolving capacity and good flowing and transferring performance. The flow of the supercritical fluid can influence the extraction effect of the birch powder, the flow is limited to avoid overlarge flow, the supercritical fluid carries away the birch powder, the content of the birch powder is reduced, and the extraction rate of effective components is further reduced; the problem that the effective components in the birch powder are difficult to extract due to over-small flow is avoided, or even if the effective components can be extracted, the extraction time is long; under the condition of the flow rate, the supercritical fluid has the best extraction effect on the effective components in the birch powder.
Because carbon dioxide has the advantages of no toxicity, no combustion, no reaction to most substances, low price and the like, and CO is generated in a supercritical state2The fluid has the double characteristics of gas phase and liquid phase, has high diffusion coefficient and low viscosity equivalent to that of gas, has density similar to that of liquid and good dissolving capacity of matter, and has density sensitive to temperature and pressure change and proportional to the dissolving capacity in certain pressure range, so that the solubility of matter may be changed through controlling temperature and pressure. The supercritical fluid in this embodiment is preferably carbon dioxide, which can significantly improve the extraction rate of the effective components. Propane is stable in chemical property and is not easy to generate chemical reaction. The propane and carbon dioxide are used together, so that the solubility of the birch powder can be further improved, and the extraction rate of the effective components can be further improved.
The extraction temperature is 45-55 ℃, and the extraction pressure is 22-30 MPa. The density of the supercritical fluid at around the critical temperature and critical pressure is a function of temperature and pressure only, so at the appropriate temperature and pressure it can provide sufficient density to ensure sufficiently strong solubility. Under the temperature and pressure, the extraction rate of betulin and betulinic acid in the birch powder is highest. Ultrasonic treatment is carried out for 4-8min, and treatment is carried out once every 5-10 min. The ultrasonic treatment can change the state of the birch powder, accelerate the movement speed of the birch powder and the movement speed of the supercritical fluid, so that the supercritical fluid can better extract the effective components in the birch powder; through multiple times of ultrasonic treatment, the equilibrium state during ultrasonic extraction can be destroyed for multiple times, and the extraction rate is further improved.
The separation and purification steps of the extract comprise: adding mixed solution of ethyl acetate and chloroform into the extract, heating and refluxing for 0.5-1.5h at 40-45 ℃, filtering to obtain filtrate, and concentrating the filtrate under reduced pressure to obtain crude extract; adding 1-5 times of anhydrous ethanol into the crude extract, and recrystallizing to obtain birch extract.
The extract is subjected to a first separation by using a mixed solution of ethyl acetate and chloroform, so that impurities in the extract can be removed primarily, and then the purity of the effective components in the birch extract can be further improved through a second separation of recrystallization. Heating and refluxing for 0.5-1.5h at 40-45 deg.C to avoid loss of part of effective components due to over-high temperature, short time and unsatisfactory separation effect, so as to obtain the best primary separation effect of the extract. Adding 1-5 times of anhydrous ethanol into the crude extract for recrystallization to obtain high-purity and high-quality birch extract.
The volume ratio of ethyl acetate to chloroform in the mixed solution is 1: (0.4-0.8). The filtrate is concentrated under reduced pressure at 1.12-1.89MPa for 5-10 min. The method selects the mixed solution of ethyl acetate and chloroform for purification and separation, because the properties of betulin and betulinic acid are different, the separation effect in a single solution is poor, and the mixed solution can be selected to obtain the betulin and the betulinic acid with higher purity, so that the separation effect of the betulin and the betulinic acid is better. The mixed solution has the best purification and separation effect on the extract under the condition of the mixture ratio. The vacuum concentration is carried out for 5-10min under 1.12-1.89MPa, so that crude extract with proper solid-to-liquid ratio can be obtained, and the crude extract can be further separated and purified more easily in the subsequent recrystallization process.
After oscillating for 10-20min, adding ganoderan and lycium barbarum polysaccharide. The ganoderan and lycium barbarum polysaccharide can provide nutrient substances for organisms, improve the immune function of the organisms and further enhance the function of repairing nerve cells by food.
Shaking for 10-20min, and adding grape juice, blueberry juice or tomato juice. The grape juice, blueberry juice and tomato juice all contain a large amount of vitamins, so that the nutrition can be further supplemented for the organism, and the immune function of the organism can be further improved.
A food for repairing nerve cells is prepared from the Chinese-medicinal materials including liquorice root, etc through preparing the mixture of said materials, mixing, stirring, adding water, stirring, and shaping. The preparation type of the food can be powder, granule, tablet or liquid, and is selected according to actual conditions without limitation. By making into different types, the food can greatly expand the population of people and improve the popularity of the food.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 50 deg.C, and sieving with 80 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 20min to obtain extract; separating and purifying the extract to obtain a refined birch extract, wherein the mass ratio of the birch extract to purified water is 0.01: 5 mixing, and oscillating for 10min at 20KHz frequency.
Example 2
A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 60 deg.C, and sieving with 90 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 30min to obtain extract; separating and purifying the extract to obtain a refined birch extract, and mixing the birch extract with purified water according to a mass ratio of 1: 5 mixing, and oscillating for 20min at 30KHz frequency.
Example 3
A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 55 deg.C, and sieving with 85 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 25min to obtain extract; separating and purifying the extract to obtain a refined birch extract, wherein the mass ratio of the birch extract to purified water is 0.1: 5 mixing, and oscillating for 15min at 25KHz frequency.
Example 4
A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 55 deg.C, and sieving with 85 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 25min to obtain extract; separating and purifying the extract to obtain a refined birch extract, wherein the mass ratio of the birch extract to purified water is 0.1: 5 mixing, oscillating for 15min at 25KHz frequency, adding ganoderan and lycium barbarum polysaccharide, and mixing.
In the supercritical extraction, the flow rate of the supercritical fluid is 12kg/h, and the supercritical fluid is carbon dioxide and/or propane. During extraction, the extraction temperature is 45 ℃ and the extraction pressure is 22 MPa. During extraction, ultrasonic treatment is adopted for 4min, and treatment is carried out once every 5 min.
Example 5
A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 55 deg.C, and sieving with 85 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 25min to obtain extract; separating and purifying the extract to obtain a refined birch extract, wherein the mass ratio of the birch extract to purified water is 0.1: 5 mixing, oscillating for 15min at 25KHz frequency, adding succus Vitis Viniferae, and mixing.
The separation and purification steps of the extract comprise: adding a mixed solution of ethyl acetate and chloroform into the extract, heating and refluxing for 0.5h at 40 ℃, filtering to obtain a filtrate, and concentrating the filtrate under reduced pressure to obtain a crude extract; adding 1 time of anhydrous ethanol into the crude extract, and recrystallizing to obtain birch extract.
Example 6
A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 55 deg.C, and sieving with 85 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 25min to obtain extract; separating and purifying the extract to obtain a refined birch extract, wherein the mass ratio of the birch extract to purified water is 0.1: 5 mixing, oscillating for 15min at 25KHz frequency, adding tomato juice, and mixing.
In the supercritical extraction, the flow rate of the supercritical fluid is 18kg/h, and the supercritical fluid is carbon dioxide and/or propane. During extraction, the extraction temperature is 55 ℃ and the extraction pressure is 30 MPa. During extraction, ultrasonic treatment is adopted for 8min, and treatment is carried out once every 10 min.
The separation and purification steps of the extract comprise: adding a mixed solution of ethyl acetate and chloroform into the extract, heating and refluxing for 1.5h at 45 ℃, filtering to obtain a filtrate, and concentrating the filtrate under reduced pressure to obtain a crude extract; adding 5 times of anhydrous ethanol into the crude extract, and recrystallizing to obtain birch extract.
Example 7
A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 55 deg.C, and sieving with 85 mesh sieve to obtain birch powder; performing supercritical extraction on birch powder for 25min to obtain extract; separating and purifying the extract to obtain a refined birch extract, wherein the mass ratio of the birch extract to purified water is 0.1: 5 mixing, oscillating for 15min at 25KHz frequency, adding Ganoderma polysaccharide, fructus Lycii polysaccharide and fructus Myrtilli juice, and mixing.
In the supercritical extraction, the flow rate of the supercritical fluid is 14kg/h, and the supercritical fluid is carbon dioxide and/or propane. During extraction, the extraction temperature is 50 ℃, and the extraction pressure is 25 MPa. During extraction, ultrasonic treatment is adopted for 5min, and the treatment is carried out once every 8 min.
The separation and purification steps of the extract comprise: adding a mixed solution of ethyl acetate and chloroform into the extract, heating and refluxing for 1h at 42 ℃, filtering to obtain a filtrate, and concentrating the filtrate under reduced pressure to obtain a crude extract; adding 3 times of anhydrous ethanol into the crude extract, and recrystallizing to obtain birch extract.
Test results
1. Betulin quantitative analysis method
(1) Determination of betulin content in birch bark
Accurately weighing 1.00g of birch powder of examples 1-7 of the invention, respectively adding into a Soxhlet extractor, continuously extracting for 24h with 100mL of absolute ethyl alcohol as solvent, transferring the extract into a 250mL volumetric flask, diluting to scale, and mixing uniformly. Collecting 1mL extractive solution with constant volume, centrifuging (12000r/min) for 5min, collecting 10 μ L supernatant, determining and calculating betulin concentration (g/L) in sample by chromatography, and calculating according to W1＝[(C1×V1)/M1]X 100% calculation of betulin content (W)1). The mass fraction of betulin in the birch bark sample was found to be 24.85 ± 0.87%.
Wherein M is1Sample quality of birch bark (g), C1The mass concentration (g/L) of betulin in the sample; v1The volume of the sample extract was 0.25 (L).
(2) Determination of betulin content in birch extract
Accurately weighing birch extract 0.100g of the birch extracts of examples 1-7 of the invention, adding ethanol to a constant volume of 50mL, shaking up, sucking 1.0mL into a centrifuge tube, centrifuging (12000r/min) for 5min, and sampling 10 μ L of supernatant for determination.
Betulin extraction Rate (W)2) The calculation formula is as follows:
W2＝[(C2×V2×m1)/(m2×W1×M2)]×100％
wherein M is2The weight (g) of the birch bark; m is1The weight (g) of birch extract; m is2Birch extract sample mass (g); c2The mass concentration (g/L) of betulin in the extract sample; v2Extract sample solution volume 0.05 (L); w1The content of betulin in cortex Betulae Pendulae is 25.34%.
TABLE 1 betulin extraction Rate
As can be seen from table 1, the betulin extraction rate in the birch extracts of examples 1-7 of the present invention is 64% -80%, whereas the betulin extraction rate is highest under the operating conditions of example 7. Thus, example 7 is the best condition for betulin extraction.
2. Cell assay
The test method comprises the following steps: selecting 7 parts of rat adrenal pheochromocytoma PC12 cells, respectively culturing PC12 cells with 10% fetal calf serum RPMI1640 culture solution to a logarithmic growth period, digesting with 0.25% trypsin-disodium edetate digestive juice, respectively adding 10% calf serum culture solution to dilute to different concentrations, then spreading the cell suspension on a cell culture plate, and placing in a specific culture box for culturing for 24 h. Then adding glutamic acid with the final concentration of 30mmol/L into the incubator for 24h, and causing damage to PC12 cells. PC 12-damaged cells were cultured in a new incubator as described above, 1 to 7 portions of the food products corresponding to examples 1 to 7 using the present invention were added to the incubator, respectively, at a food concentration of 10. mu.g/L, and cultured for 24 hours. After 24h, the measurement is carried out by using an MTT method,
the cell repair rate is obtained. TABLE 2 different groups of PC12 cell repair rates
As is apparent from Table 2, the food of the present invention was able to significantly repair nerve cell damage, and among them, the food of example 7 had the best repairing effect compared to the foods of examples 1 to 6.
In summary, in the food for repairing nerve cells according to the embodiment of the present invention, the higher the betulin content, the better the repairing effect of the food on nerve cells. Meanwhile, the food can effectively repair nerve cells, improve the function of the nerve cells, provide organism nutrition, strengthen the immunity function of the organism and better repair the nerve cells.
The preparation method of the food for repairing nerve cells, provided by the embodiment of the invention, is simple and easy to operate, and is beneficial to large-scale production.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (10)
1. A method for preparing a food for repairing nerve cells, comprising the steps of:
micronizing cortex Betulae Pendulae at 50-60 deg.C, and sieving with 80-90 mesh sieve to obtain birch powder; performing supercritical extraction on the birch powder for 20-30min to obtain an extract; separating and purifying the extract to obtain a refined birch extract, wherein the birch extract and purified water are mixed according to a mass ratio (0.01-1): 5 mixing, and oscillating for 10-20min at 20-30KHz frequency.
2. The preparation method according to claim 1, wherein the flow rate of the supercritical fluid is 12 to 18kg/h in the supercritical extraction, and the supercritical fluid is carbon dioxide and/or propane.
3. The process according to claim 1, wherein the supercritical extraction is carried out at an extraction temperature of 45 to 55 ℃ and an extraction pressure of 22 to 30 MPa.
4. The method of claim 1, wherein the supercritical extraction further comprises ultrasonic treatment for 4-8min, and the treatment is performed every 5-10 min.
5. The method according to claim 1, wherein the step of separating and purifying the extract comprises: adding a mixed solution of ethyl acetate and chloroform into the extract, heating and refluxing for 0.5-1.5h at 40-45 ℃, filtering to obtain a filtrate, and concentrating the filtrate under reduced pressure to obtain a crude extract; adding 1-5 times of anhydrous ethanol into the crude extract, and recrystallizing to obtain the birch extract.
6. The method according to claim 5, wherein the volume ratio of ethyl acetate to chloroform in the mixed solution is 1: (0.4-0.8).
7. The method according to claim 5, wherein the concentration of the filtrate under reduced pressure is a concentration at 1.12 to 1.89MPa for 5 to 10 min.
8. The method according to claim 1, further comprising adding ganoderan and lycium barbarum polysaccharide after shaking for 10-20 min.
9. The method of claim 1, further comprising adding grape juice, blueberry juice or tomato juice after shaking for 10-20 min.
10. A food for repairing nerve cells, which is prepared by the method for preparing a food for repairing nerve cells according to any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111361830.3A CN114009780A (en) | 2021-11-17 | 2021-11-17 | Food for repairing nerve cells and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111361830.3A CN114009780A (en) | 2021-11-17 | 2021-11-17 | Food for repairing nerve cells and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114009780A true CN114009780A (en) | 2022-02-08 |
Family
ID=80064964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111361830.3A Pending CN114009780A (en) | 2021-11-17 | 2021-11-17 | Food for repairing nerve cells and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114009780A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1712510A (en) * | 2004-06-23 | 2005-12-28 | 杜新刚 | Glossy ganoderma and matrimony vine tomato wine |
| CN1810826A (en) * | 2006-02-21 | 2006-08-02 | 东北林业大学 | Betulinol producing process |
| CN105029166A (en) * | 2015-06-10 | 2015-11-11 | 周爱民 | Compound type multifunctional food and preparation method thereof |
| CN105106298A (en) * | 2015-09-09 | 2015-12-02 | 济南星懿医药技术有限公司 | Pharmaceutical composition with nerve cell protective effect |
| CN105533367A (en) * | 2015-12-22 | 2016-05-04 | 内蒙古满洲里森诺生物科技有限公司 | White birch bark extract beverage and preparation method thereof |
| KR101802092B1 (en) * | 2016-10-13 | 2017-11-27 | 강원대학교산학협력단 | Compostion for prevention or treatment of dementia comprising Euonymus alatus Sieb. extracts or fraction comprising methyl hydrogen tetradecanedioate or pharmaceutically acceptable salts thereof |
| CN111494390A (en) * | 2020-05-18 | 2020-08-07 | 南通大学 | New application of betulin derivative in preparation of nerve injury repairing medicine |
-
2021
- 2021-11-17 CN CN202111361830.3A patent/CN114009780A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1712510A (en) * | 2004-06-23 | 2005-12-28 | 杜新刚 | Glossy ganoderma and matrimony vine tomato wine |
| CN1810826A (en) * | 2006-02-21 | 2006-08-02 | 东北林业大学 | Betulinol producing process |
| CN105029166A (en) * | 2015-06-10 | 2015-11-11 | 周爱民 | Compound type multifunctional food and preparation method thereof |
| CN105106298A (en) * | 2015-09-09 | 2015-12-02 | 济南星懿医药技术有限公司 | Pharmaceutical composition with nerve cell protective effect |
| CN105533367A (en) * | 2015-12-22 | 2016-05-04 | 内蒙古满洲里森诺生物科技有限公司 | White birch bark extract beverage and preparation method thereof |
| KR101802092B1 (en) * | 2016-10-13 | 2017-11-27 | 강원대학교산학협력단 | Compostion for prevention or treatment of dementia comprising Euonymus alatus Sieb. extracts or fraction comprising methyl hydrogen tetradecanedioate or pharmaceutically acceptable salts thereof |
| CN111494390A (en) * | 2020-05-18 | 2020-08-07 | 南通大学 | New application of betulin derivative in preparation of nerve injury repairing medicine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105648021B (en) | The preparation method of ginseng rare saponin(e C-K, F1 and four kinds of isomers ginsengenins | |
| CN109400664B (en) | Method for extracting rice oil phytosterol | |
| CN114573421B (en) | Method for extracting and purifying sclareol from sclareol fermentation liquor | |
| CN110467521A (en) | It is a kind of using industrial hemp as cannabidiol (CBD) isolation and purification method of raw material | |
| CN111072449B (en) | A method for preparing natural ferulic acid from nigre containing oryzanol | |
| CN102925497A (en) | Method for preparing high-purity resveratrol from polygonum cuspidatum | |
| CN116042342A (en) | Ginseng juice wine and production process thereof | |
| CN111117773A (en) | Method for separating nervonic acid from garlic oil and application thereof | |
| CN114009780A (en) | Food for repairing nerve cells and preparation method thereof | |
| CN104366332A (en) | Deep processing method of chilli seeds | |
| JP5399468B2 (en) | Composition exhibiting lipolytic activity | |
| JP2008212137A (en) | Method for producing new gisenoside from ginseng by liquid culture of phellinus linteus mycelium utilizing biotransformation method | |
| CN101077851B (en) | Method for extracting D-chiro-inositol from buckwheat husks | |
| CN108101869A (en) | A kind of extracting method of natural Japanese yew alcohol | |
| CN110507749B (en) | Mongolian leek anti-tumor extract and preparation method and application thereof | |
| CN102190689A (en) | Purification method for ceramide in amorphophalms konjac | |
| TW202128202A (en) | Use of cyclocarya paliurus extract for enhancing the expression level of fat loss gene, increasing basal metabolic rate, and/or suppressing fat accumulation | |
| CN112654631A (en) | Novel ginsenoside and anti-inflammatory composition containing same | |
| CN116947589B (en) | Extraction and purification method for biosynthesis squalene | |
| CN108586435A (en) | The preparation method of Echinulin | |
| CN109771468A (en) | Eucommia Bark male flower active component and its preparation method and application | |
| CN112794812B (en) | Alkaloid compound extracted from banana flower and extraction method thereof | |
| JP6606636B2 (en) | Betulin derivative exhibiting cell membrane stabilizing action and production method thereof | |
| JP2011217696A (en) | Method for producing pinitol or pinitol-containing composition, and microorganism used for the same | |
| KR20130074118A (en) | Ginseng prosapogenin high concentration containing ginseng berry preparation using sonication and process for thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |