CN114009449A - Method for increasing number of root nodules with nitrogenase activity on roots of leguminous crops and application of root nodules on leguminous crops - Google Patents
Method for increasing number of root nodules with nitrogenase activity on roots of leguminous crops and application of root nodules on leguminous crops Download PDFInfo
- Publication number
- CN114009449A CN114009449A CN202111217143.4A CN202111217143A CN114009449A CN 114009449 A CN114009449 A CN 114009449A CN 202111217143 A CN202111217143 A CN 202111217143A CN 114009449 A CN114009449 A CN 114009449A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- crops
- leguminous crops
- leguminous
- increasing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for increasing the number of root nodules with nitrogenase activity in leguminous plants and the application thereof in leguminous crops. Mixing 2 or 3 of bacillus amyloliquefaciens, bacillus brevis and bacillus mucilaginosus with the enterobacter ledwigii, applying the mixture to the field of leguminous crops, and increasing the number of root nodules with the azotobacter activity of the leguminous crops. The method is applied to increase the number of the root nodules with the nitrogenase activity on the roots of leguminous crops such as peanuts and the like, is simple to use, has obvious yield increase benefit and ecological benefit, and is easy to popularize and apply.
Description
Technical Field
The invention relates to a method for increasing the number of root nodules with nitrogenase activity of leguminous crops and application of the root nodules to the leguminous crops.
Background
When leguminous crops such as peanuts and the like grow in fields, roots can be infected by rhizobia to form nodules, and if the nodules have azotobacter activity, free nitrogen can be fixed and nitrogen-containing compounds can be synthesized and utilized by the leguminous plants, so that high yield is obtained, and the method has very important practical significance for fertilizer reduction, carbon peak reaching and carbon neutralization. If the root nodules are dense, the phenomenon is called super-nodulation. The phenomenon of super-nodulation is still extremely rare at present. Therefore, how to produce a large number of nodules with nitrogenase activity in leguminous crops such as peanuts? This has been a hot problem of worldwide research.
In the practical production, due to the long-term large-scale use of chemical fertilizers in farmlands, crops such as peanuts and the like have few root nodules, and only a few root nodules are usually visible from main roots. In order to increase the number of root nodules in crops, much research has focused on rhizobia, developing superior rhizobia, but there are few that can be applied to production and play a significant role at present.
Aiming at the problems, through deep research, an inventor group constructs a peanut pod internal and rhizosphere microorganism strain library, namely peanut endophyte and peanut exohyte, on the basis, a method for increasing the number of the root nodule with the azotobacter activity of leguminous crops is successfully invented, a key method support is provided for increasing the number of the root nodule with the azotobacter activity of leguminous crops such as peanuts and promoting yield increase, and the method has very important significance for farmland fertilizer reduction, carbon peak reaching and carbon neutralization.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the method for increasing the number of the root nodules with the nitrogenase activity of the leguminous crops and the application of the method in the leguminous crops.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a method for increasing the number of the root nodules with the nitrogenase activity of leguminous plants comprises the steps of mixing 2 or 3 of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Bacillus laterosporus (Brevibacillus laterosporus) and Bacillus mucilaginosus (Bacillus mucilaginosus Krassilnikov) with Enterobacter ledwigii (Enterobacter ludwigii), applying the mixture to the field of leguminous plants, and increasing the number of the root nodules with the nitrogenase activity of the leguminous plants.
According to the scheme, the method comprises the following steps: batch culture of 4 microbial strains: (1) bacillus amyloliquefaciens, (2) brevibacillus laterosporus, (3) bacillus mucilaginosus, (4) enterobacter ledebensis, any 2 or 3 of microbial strains (1) bacillus amyloliquefaciens, (2) brevibacillus laterosporus, (3) bacillus mucilaginosus and microbial strains (4) enterobacter ledebensis are mixed and applied to the field of leguminous crops, and the number of the root nodule with the azotobacter activity of the leguminous crops is increased.
According to the scheme, the batch culture can be a conventional method for fermenting bacillus or a conventional method for amplifying and culturing a bacterial culture medium.
According to the scheme, the 4 microbial strains can be obtained commercially or obtained by screening and separating by a conventional method, and the screening and separating can be obtained by separating peanut pods or rhizosphere soil, or can be obtained by separating roots or rhizosphere soil of other crops such as soybeans and the like.
According to the scheme, the mixing proportion refers to that the ratio of the number of viable bacteria of any one strain after mixing is more than or equal to 1%.
According to the scheme, the mixed microbial strains can be applied to the fields of leguminous crops along with the crop base fertilizer, and can also be applied to the fields in the growing period of the crops, and the preferable application amount is more than or equal to 1000 hundred million viable bacteria per mu.
According to the scheme, the leguminous crops comprise but are not limited to peanuts, soybeans, alfalfa, astragalus sinicus and the like.
The invention also provides application of the method for increasing the number of the root nodules with the nitrogenase activity of the leguminous plants in increasing the number of the root nodules with the nitrogenase activity of the leguminous plants and increasing the yield of leguminous crops.
Furthermore, the invention also provides the application of the method for increasing the number of the root nodules with the nitrogenase activity of the leguminous plants in inducing the super nodulation phenomenon of the leguminous plants.
The invention has the beneficial effects that:
1. the method can be used for increasing the number of the root nodules with the nitrogenase activity of the roots of leguminous crops such as peanuts, soybeans, alfalfa, milk vetch and the like, and inducing the root nodules to appear in a super mode, and 2, the method is easy to operate, strong in practicability and easy to popularize and apply, 3, the method is favorable for promoting the yield increase of the crops and has economic benefits, 4, the method is favorable for improving the soil environment of farmlands and ecological benefits, and 5, the method is favorable for reducing the application of farmland fertilizers, and has important significance for carbon peak reaching and carbon neutralization.
Drawings
FIG. 1 is a diagram of root nodule conditions of peanuts treated by the method of the present invention, wherein the left diagram is a comparison, the right diagram is the method of the present invention, and the right diagram shows the super nodule phenomenon generated by the method of the present invention;
FIG. 2 is a graph of the growth of a peanut plant treated in example 1 by the method of the present invention. The left figure is a comparison, and the right figure is a growth chart of peanut crops treated by mixing bacillus amyloliquefaciens, bacillus brevis, bacillus mucilaginosus and enterobacter ledwigii.
Detailed Description
EXAMPLE 1 establishment of a method for increasing the number of root nodules having nitrogenase activity on peanut roots
Method for establishing root nodule number capable of obviously increasing azotase activity of peanut root by following steps
(1) A peanut pod and rhizosphere soil sample is obtained from a peanut producing area in Guangxi province, strains are separated from peanut pods or rhizosphere soil through a conventional bacteria separation method, or strains are separated from roots or rhizosphere soil of other crops such as soybeans and the like, and series of bacteria such as bacillus amyloliquefaciens, bacillus laterosporus, bacillus mucilaginosus, enterobacter ledebensis and the like are obtained through conventional 16s rDNA method identification. And then the obtained series of bacterial strains are subjected to amplification culture by a conventional bacterial culture medium or a conventional bacillus fermentation method to prepare and obtain batches of bacillus amyloliquefaciens, bacillus laterosporus, bacillus mucilaginosus, enterobacter ladiei and the like.
(2) The idea of combining peanut endophytic strains with exophytic strains is adopted, 2, 3 and 4 strains are mixed to form a series of microbial combinations, wherein the ratio of the viable count of any one strain in each microbial combination is more than or equal to 1%.
(3) The microorganism combination is applied to the field along with the peanut seeding base fertilizer or the field in the growth period of the peanuts, and the application amount is more than or equal to 1000 hundred million viable bacteria per mu.
(4) The test results are investigated 3-45 days before peanut harvest, and the number of root nodules of the peanuts is obviously increased by more than 30 times in the following treatment fields with a plurality of microbial strain variety combinations compared with control fields without microbial combinations: microorganism species combination 157-Bacillus amyloliquefaciens, Brevibacillus laterosporus, Bacillus mucilaginosus, Enterobacter ludwigii; microorganism species combination 033-Brevibacillus laterosporus, Bacillus mucilaginosus, Enterobacter ludwigii; microorganism species combination 037-bacillus amyloliquefaciens, bacillus mucilaginosus, enterobacter ledwigii; the combination 039 of microbial species, i.e., bacillus amyloliquefaciens, bacillus brevis, enterobacter ledwigii, even large-area super nodulation phenomenon occurs, and the nodules have the azotase activity through the conventional azotase activity determination method. While none or little of the other combinations showed an increase in the number of nodules. In addition, the growth vigor of the peanuts in the fields treated by the microorganism combinations 157, 033, 037 and 039 is obviously superior to that of the control fields, and the yield of each mu is increased by 19%. The yield of the microorganism variety combination 157 can reach 5 to 19 percent per mu. The yield increase rate of the microorganism variety combination 033 per mu can reach 3-16%. The microbial species combination 037 can increase the yield by 4-12% per mu. The microbial species combination 039 can increase the yield by 3-18% per mu.
Based on the research results, a method for increasing the number of the root nodule with the azozyme activity is established, namely, two or three of the bacillus amyloliquefaciens, the bacillus laterosporus and the bacillus mucilaginosus are mixed with the enterobacter ludwigii, and the mixture is applied to the field along with the peanut seeding base fertilizer or the field in the growth period of the peanuts, and the application amount is more than or equal to 1000 hundred million viable bacteria per mu. Research results show that the method can be used for increasing the number of the root nodules with the azotase activity of the peanut roots by more than 30 times, is easy to operate, strong in practicability and popularize and apply, is beneficial to promoting the yield increase of crops, and the yield increase can reach 3% -19%, has economic benefits, is also beneficial to improving the soil environment of farmlands, has ecological benefits, has important significance for reducing the application of farmlands and fertilizer, and has great significance for carbon peak reaching and carbon neutralization.
EXAMPLE 2 establishment of a method for increasing the number of root nodules having nitrogenase activity on peanut roots (II)
Method for establishing root nodule number capable of obviously increasing azotase activity of peanut root by following steps
(1) A peanut pod and rhizosphere soil sample is obtained from a Jiangsu flower production area, strains are separated from the peanut pod or the rhizosphere soil through a conventional bacteria separation method, or the strains are separated from the roots of other crops such as soybeans or the rhizosphere soil, and series of bacteria such as bacillus amyloliquefaciens, bacillus laterosporus, bacillus mucilaginosus, enterobacter ledebensis and the like are obtained through conventional 16s rDNA method identification. And then the obtained series of bacterial strains are subjected to amplification culture by a conventional bacterial culture medium or a conventional bacillus fermentation method to prepare and obtain batches of bacillus amyloliquefaciens, bacillus laterosporus, bacillus mucilaginosus, enterobacter ladiei and the like.
(2) The idea of combining peanut endophytic strains with exophytic strains is adopted, 2, 3 and 4 strains are mixed to form a series of microbial combinations, wherein the ratio of the viable count of any one strain in each microbial combination is more than or equal to 1%.
(3) The microorganism combination is applied to the field along with the peanut seeding base fertilizer or the field in the growth period of the peanuts, and the application amount is more than or equal to 1000 hundred million viable bacteria per mu.
(4) The test results are investigated 3-45 days before peanut harvest, and the root nodule number of the peanut is obviously more than that of a control field without the microbial combination in the following treatment fields with the microbial strain combination (the nodule number is increased by more than 30 times): microbial species combination 156-Bacillus amyloliquefaciens, Brevibacillus laterosporus, Bacillus mucilaginosus, Enterobacter ludwigii; microorganism species combination 032-Brevibacillus laterosporus, Bacillus mucilaginosus, Enterobacter ludwigii; microorganism species combination 036-bacillus amyloliquefaciens, bacillus mucilaginosus, enterobacter ledwigii; the combination 038 of microbial species, i.e., bacillus amyloliquefaciens, bacillus brevis, enterobacter ledwigii, even large-area super nodulation phenomenon occurs, and the nodules have the azotase activity through the conventional azotase activity determination method. While none or little of the other combinations showed an increase in the number of nodules. In addition, the growth vigor of the peanuts in the fields treated by the microorganism combinations 156, 032, 036 and 038 is obviously superior to that of the control fields, and the yield of each mu is increased by 18%. The microbial combination 156 can increase the yield by 3-18% per mu. The yield of the microorganism combination 032 can be increased by 3-16% per mu. The microbial combination 036 has a yield increase of 4-15% per mu. The microbial combination 038 can increase the yield by 3-12% per mu.
Based on the research results, a method for increasing the number of the root nodule with the azozyme activity is established, namely the bacillus amyloliquefaciens, the brevibacillus laterosporus, the bacillus mucilaginosus and the enterobacter ludwigii are mixed and applied to the field along with the peanut seeding base fertilizer or applied to the field in the peanut growing period, and the application amount is more than or equal to 1000 hundred million viable bacteria per mu. Research results show that the method can be used for increasing the number of the root nodules with the azotase activity of the peanut roots by more than 30 times, is easy to operate, strong in practicability and popularize and apply, is beneficial to promoting the yield increase of crops, and the yield increase rate can reach 7.7 percent on average, has economic benefit, is also beneficial to improving the soil environment of farmland, has ecological benefit, and has important significance for reducing the application of farmland fertilizers and carbon peak reaching and carbon neutralization.
EXAMPLE 3 establishment of a method for increasing the number of root nodules having nitrogenase activity on peanut roots (III)
Method for establishing root nodule number capable of obviously increasing azotase activity of peanut root by following steps
(1) A peanut pod and rhizosphere soil sample is obtained from a peanut producing area in Hebei, strains are separated from peanut pods or rhizosphere soil through a conventional bacteria separation method, or strains are separated from roots or rhizosphere soil of other crops such as soybeans and the like, and series of bacteria such as bacillus amyloliquefaciens, bacillus laterosporus, bacillus mucilaginosus, Enterobacter ludwigii and the like are obtained through conventional 16s rDNA method identification. And then the obtained series of bacterial strains are subjected to amplification culture by a conventional bacterial culture medium or a conventional bacillus fermentation method to prepare and obtain batches of bacillus amyloliquefaciens, bacillus laterosporus, bacillus mucilaginosus, enterobacter ladiei and the like.
(2) The idea of combining peanut endophytic strains with exophytic strains is adopted, 2, 3 and 4 strains are mixed to form a series of microbial combinations, wherein the ratio of the viable count of any one strain in each microbial combination is more than or equal to 1%.
(3) The microorganism combination is applied to the field along with the peanut seeding base fertilizer or the field in the growth period of the peanuts, and the application amount is more than or equal to 1000 hundred million viable bacteria per mu.
(4) The test results are investigated 3-45 days before peanut harvest, and the root nodule number of the peanut is obviously more than that of a control field without the microbial combination in the following treatment fields with the microbial strain combination (the nodule number is increased by more than 50 times): microorganism species combination 154-Bacillus amyloliquefaciens, Brevibacillus laterosporus, Bacillus mucilaginosus, Enterobacter ludwigii; microorganism species combination 030-Brevibacillus laterosporus, Bacillus mucilaginosus, Enterobacter ludwigii; microorganism species combination 034-bacillus amyloliquefaciens, bacillus mucilaginosus, enterobacter ledwigii; the microorganism species combination 032, namely bacillus amyloliquefaciens, bacillus brevis and enterobacter ludwigii, even has large-area super nodulation phenomenon, and the nodulation has the azotase activity after being determined by a conventional azotase activity determination method. While none or little of the other combinations showed an increase in the number of nodules. In addition, the growth vigor of the peanuts in the fields treated by the microbial combinations 154, 030, 034 and 032 is obviously superior to that of the control fields, and the yield of each mu is increased by 18%. The microbial composition 154 can increase the yield by 5-17% per mu. The microbial combination 030 has a yield increase rate of 5-17% per mu. The microbial combination 034 can increase the yield by 5-13% per mu. The yield increase rate of the field treated by the microorganism combination 032 per mu can reach 5-18%.
Based on the research results, a method for increasing the number of the root nodule with the azozyme activity is established, namely the bacillus amyloliquefaciens, the brevibacillus laterosporus, the bacillus mucilaginosus and the enterobacter ludwigii are mixed and applied to the field along with the peanut seeding base fertilizer or applied to the field in the peanut growing period, and the application amount is more than or equal to 1000 hundred million viable bacteria per mu. Research results show that the method can be used for increasing the number of the root nodules with the azotase activity of the peanut roots by more than 50 times, is easy to operate, strong in practicability and popularize and apply, is beneficial to promoting the yield increase of crops by more than 5%, has economic benefits, is also beneficial to improving the soil environment of farmlands, has ecological benefits, has important significance for reducing the application of farmlands and achieving peak carbon and carbon neutralization.
The above results show that: according to the invention, two or three of bacillus amyloliquefaciens, bacillus brevis and bacillus mucilaginosus and the enterobacter ledwigii are mixed for use, so that the quantity of the root nodules with the azotobacter activity on the peanut roots can be increased, and the effects of promoting the yield increase of crops and the like are facilitated. The obtaining method of the bacillus amyloliquefaciens, the brevibacillus laterosporus, the bacillus mucilaginosus and the enterobacter ledwigii is not limited by adopting screening or market purchase and the like.
Example 4 application (one)
The microorganism species combination 157 of example 1, the microorganism species combination 156 of example 2, and the microorganism species combination 154 of example 3 were applied to 15 peanut primary producing areas in the whole country, such as Hubei, Henan, and Shandong, respectively. The results of the exemplary application show: the average number of the peanut nodules per plant in the demonstration area is increased by more than 30 times, the yield increase rate per mu is 3.3% -19.4%, and remarkable social and economic benefits and ecological benefits are achieved.
Example 5 application (two)
The microorganism species combination 157 of example 1, the microorganism species combination 156 of example 2, and the microorganism species combination 154 of example 3 were applied to crops such as soybean, alfalfa, and milk vetch, respectively, and all showed the phenomena of increasing the number of nodules and promoting the increase of crop yield.
Based on the constructed peanut endogenous and exogenous strain libraries, the invention discovers that the mixture of two or three of the Enterobacter ludwigii, the Bacillus amyloliquefaciens, the Brevibacillus laterosporus and the Bacillus mucilaginosus can effectively increase the number of the root nodules with the azotobacter activity of the leguminous crops and even induce the super nodulation phenomenon at the root of the peanut after the mixture is interacted with the leguminous crops, the rhizobia and the rhizosphere environment, thereby successfully inventing a method for increasing the number of the root nodules with the azotobacter activity of the leguminous crops such as the peanut, the soybean, the alfalfa, the astragalus sinicus and the like to increase the number of the root nodules, promoting the yield increase and providing key technical supports for the fertilizer reduction of the application of farmland, the carbon peak reaching and the carbon neutralization.
Claims (9)
1. A method for increasing the number of nodules having nitrogenase activity in the roots of leguminous plants, characterized in that: mixing 2 or 3 of bacillus amyloliquefaciens, bacillus brevis and bacillus mucilaginosus with the enterobacter ledwigii, applying the mixture to the field of leguminous crops, and increasing the number of root nodules with the azotobacter activity of the leguminous crops.
2. The method of claim 1, wherein: batch culture of 4 microbial strains: (1) bacillus amyloliquefaciens, (2) brevibacillus laterosporus, (3) bacillus mucilaginosus, (4) enterobacter ledebensis, any 2 or 3 of microbial strains (1) bacillus amyloliquefaciens, (2) brevibacillus laterosporus, (3) bacillus mucilaginosus and microbial strains (4) enterobacter ledebensis are mixed and applied to the field of leguminous crops, and the number of the root nodule with the azotobacter activity of the leguminous crops is increased.
3. The method of claim 1, wherein: the batch culture can be a conventional method for fermenting bacillus or a conventional method for amplifying culture of a bacterial culture medium.
4. The method of claim 1, wherein: the 4 microbial strains can be obtained commercially, or obtained by screening and separating by a conventional method, wherein the screening and separating are obtained by separating from peanut pods or rhizosphere soil, or separating from roots or rhizosphere soil of other crops.
5. The method of claim 1, wherein: the mixing proportion refers to the proportion of viable count of any one strain after mixing being more than or equal to 1 percent.
6. The method of claim 1, wherein: the mixed microbial strains can be applied to the field along with the crop base fertilizer or applied to the field in the crop growth period when the mixed microbial strains are applied to the field of leguminous crops, and the preferable application amount is more than or equal to 1000 hundred million viable bacteria per mu.
7. The method of claim 1, wherein: the leguminous crops include but are not limited to peanuts, soybeans, alfalfa and milk vetch.
8. Use of the method of claim 1 for increasing the number of nodules having nitrogenase activity in the roots of legumes and for increasing yield in legumes.
9. Use of the method of claim 1 for inducing a hypersarcoma phenomenon in leguminous plants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111217143.4A CN114009449B (en) | 2021-10-19 | 2021-10-19 | Method for increasing root nodule number with nitrogen fixation enzyme activity of root of leguminous crops and application of root nodule number with nitrogen fixation enzyme activity of root nodule number of leguminous crops in leguminous crops |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111217143.4A CN114009449B (en) | 2021-10-19 | 2021-10-19 | Method for increasing root nodule number with nitrogen fixation enzyme activity of root of leguminous crops and application of root nodule number with nitrogen fixation enzyme activity of root nodule number of leguminous crops in leguminous crops |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114009449A true CN114009449A (en) | 2022-02-08 |
| CN114009449B CN114009449B (en) | 2023-06-20 |
Family
ID=80056501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111217143.4A Active CN114009449B (en) | 2021-10-19 | 2021-10-19 | Method for increasing root nodule number with nitrogen fixation enzyme activity of root of leguminous crops and application of root nodule number with nitrogen fixation enzyme activity of root nodule number of leguminous crops in leguminous crops |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114009449B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0227336A2 (en) * | 1985-12-02 | 1987-07-01 | Imperial Oil Limited | Nodulation promoting bacteria and use thereof |
| US4878936A (en) * | 1987-07-27 | 1989-11-07 | Wisconsin Alumni Research Foundation | Method to enhance root nodulation in legumes and inoculum preparation therefor |
| CN105586300A (en) * | 2016-03-18 | 2016-05-18 | 中国农业科学院油料作物研究所 | Enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus |
| US20160192660A1 (en) * | 2013-08-12 | 2016-07-07 | Bio-Cat Microbials Llc | Compositions Comprising Bacillus Strains and Methods of Use to Suppress the Activities and Growth of Fungal Plant Pathogens |
| CN106011005A (en) * | 2016-05-20 | 2016-10-12 | 东莞市保得生物工程有限公司 | Bacillus amyloliquefaciens T600 and preparation method and application of microbial agent |
| WO2018012815A1 (en) * | 2016-07-12 | 2018-01-18 | 주식회사경농 | Composition for promoting growth of plants by using bacillus amyloliquefaciens, and use thereof |
| CN108624528A (en) * | 2018-05-14 | 2018-10-09 | 华中农业大学 | A kind of composite bacteria agent and its application to legume with growth-promoting production-increasing function |
| KR20190022967A (en) * | 2017-08-23 | 2019-03-07 | 경기대학교 산학협력단 | Mixed strain having nitrogen fixation activity, microbial agent containing the same and biofertilizer containing the same |
| WO2019208971A1 (en) * | 2018-04-25 | 2019-10-31 | 주식회사경농 | Bacillus velezensis strain having nitrogen fixing ability and plant growth promoting activity, and use thereof |
| CN111117910A (en) * | 2019-12-27 | 2020-05-08 | 江西农业大学 | Enterobacter ludwigii PN6 and application thereof |
-
2021
- 2021-10-19 CN CN202111217143.4A patent/CN114009449B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0227336A2 (en) * | 1985-12-02 | 1987-07-01 | Imperial Oil Limited | Nodulation promoting bacteria and use thereof |
| US4878936A (en) * | 1987-07-27 | 1989-11-07 | Wisconsin Alumni Research Foundation | Method to enhance root nodulation in legumes and inoculum preparation therefor |
| US20160192660A1 (en) * | 2013-08-12 | 2016-07-07 | Bio-Cat Microbials Llc | Compositions Comprising Bacillus Strains and Methods of Use to Suppress the Activities and Growth of Fungal Plant Pathogens |
| CN105586300A (en) * | 2016-03-18 | 2016-05-18 | 中国农业科学院油料作物研究所 | Enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus |
| CN106011005A (en) * | 2016-05-20 | 2016-10-12 | 东莞市保得生物工程有限公司 | Bacillus amyloliquefaciens T600 and preparation method and application of microbial agent |
| WO2018012815A1 (en) * | 2016-07-12 | 2018-01-18 | 주식회사경농 | Composition for promoting growth of plants by using bacillus amyloliquefaciens, and use thereof |
| KR20190022967A (en) * | 2017-08-23 | 2019-03-07 | 경기대학교 산학협력단 | Mixed strain having nitrogen fixation activity, microbial agent containing the same and biofertilizer containing the same |
| WO2019208971A1 (en) * | 2018-04-25 | 2019-10-31 | 주식회사경농 | Bacillus velezensis strain having nitrogen fixing ability and plant growth promoting activity, and use thereof |
| CN108624528A (en) * | 2018-05-14 | 2018-10-09 | 华中农业大学 | A kind of composite bacteria agent and its application to legume with growth-promoting production-increasing function |
| CN111117910A (en) * | 2019-12-27 | 2020-05-08 | 江西农业大学 | Enterobacter ludwigii PN6 and application thereof |
Non-Patent Citations (7)
| Title |
|---|
| ARCHANA DHOLE 等: "Endophytic occupation of legume root nodules by nifH-positive non-rhizobial bacteria, and their efficacy in the groundnut(Arachis hypogaea)", 《ANN MICROBIOL》 * |
| CHUNMEI DU等: "Antagonistic effect of Brevibacillus laterosporus BL-21 on pathogenic fungi of soybean root rot", 《ADVANCED ENGINEERING AND TECHNOLOGY II》 * |
| WINSTON FRANZ RÍOS-RUIZ 等: "Utilization of Endophytic Bacteria Isolated from Legume Root Nodules for Plant Growth Promotion", 《FEILD CROPS:SUSTAINABLE MANAGEMENT BY PGPR》 * |
| YANPO YAO 等: "The microbial population structure and function of peanut peanut and their effects on aflatoxin contamination", 《LWT -FOODSCIENCEANDTECHNOLOGY》 * |
| 吴洪生等: "钾细菌制剂在花生上的应用研究", 《广西农业生物科学》 * |
| 周扬 等: "黄曲霉毒素阻控与花生超级结瘤耦合效应研究初报", 《中国油料作物学报》 * |
| 崔福来 等: "几种大豆根际土壤细菌对大豆根瘤菌接种效果的影响", 《土壤通报》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114009449B (en) | 2023-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109136139B (en) | Potato endophyte and application thereof | |
| US20120192605A1 (en) | Fertilizer composition and method | |
| CN104928186A (en) | Paecilomyces lilacinus and scale preparation method thereof | |
| CN111807898A (en) | Bio-organic fertilizer with function of preventing and treating tobacco soil-borne diseases and preparation method thereof | |
| CN111646862A (en) | Special bio-organic fertilizer for hemp and preparation method thereof | |
| CN110699274A (en) | Preparation and application methods of compound microbial agent for soil improvement | |
| CN116751714B (en) | Rhizobium strain capable of inducing pigeon pea to have salt tolerance and promoting nodulation and nitrogen fixation and application thereof | |
| CN112745151A (en) | Composite biological organic fertilizer containing bacillus and trichoderma and preparation method thereof | |
| CN110229757B (en) | Trichoderma citrinoviride JS84 capable of effectively promoting crop growth and bio-organic fertilizer developed by trichoderma citrinoviride JS84 | |
| CN114009449B (en) | Method for increasing root nodule number with nitrogen fixation enzyme activity of root of leguminous crops and application of root nodule number with nitrogen fixation enzyme activity of root nodule number of leguminous crops in leguminous crops | |
| CN111039718A (en) | Agricultural streptomyces microflavus composite bacterial fertilizer taking enzymolysis cassava residues as base material and preparation method thereof | |
| CN114045247B (en) | Bacillus seawater and application thereof in salinized farmland production | |
| KR102476873B1 (en) | Bacillus toyonensis CWL-05 strain and composition for promoting plant growth comprising the same | |
| CN107974423B (en) | Soil biological activator and preparation method thereof | |
| CN114958613A (en) | Trichoderma spirillum RS05 and application thereof in preventing and treating brown rot of dendrocalamus latiflorus | |
| CN109880759B (en) | Rhizobium japonicum suitable for being prepared into freeze-dried powder and application thereof | |
| CN114058556A (en) | Preparation method of compound microbial agent | |
| Nushair et al. | Genotypic characterization of indigenous Rhizobium strain from cultivated Cowpea (Vigna unguiculata L.) in Bangladesh | |
| CN112680373A (en) | Bacillus mucilaginosus and method for preparing bio-organic fertilizer by using same through solid state fermentation | |
| Vijay et al. | Exploitation of thermophilic fungi in compost production for white button mushroom (Agaricus bisporus) cultivation-a review. | |
| Elsheikh | Effect of Salinity on Growth and Nitrogen Yield of Inoculated and N Fertilized Chickpea (Cicer aritinum) | |
| KR102559046B1 (en) | Cedecea lapagei CWL-11 strain and composition for regulating plant growth comprising the same | |
| CN115927083B (en) | Streptomyces viticola, microbial inoculum containing streptomyces viticola and application of microbial inoculum in low temperature resistance of crops | |
| CN104529661B (en) | A kind of vegetable transplanting root dipping microorganism formulation | |
| Ahmed et al. | EVALUATING THE EFFECT OF BIOFERTILIZATION IN IMPROVING GROWTH AND PRODUCTIVITY OF SOYA BEAN UNDER QANTRA SHARQ CONDITIONS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |