CN114002302A - Portable multichannel neurotransmitter detection system, method and storage medium - Google Patents
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- 238000001514 detection method Methods 0.000 title claims abstract description 77
- 239000002858 neurotransmitter agent Substances 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title abstract description 19
- 239000000523 sample Substances 0.000 claims abstract description 35
- 230000003321 amplification Effects 0.000 claims abstract description 23
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 23
- 238000012545 processing Methods 0.000 claims abstract description 9
- 230000008859 change Effects 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 6
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- 238000004590 computer program Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 5
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 25
- 229960004373 acetylcholine Drugs 0.000 description 23
- 239000000243 solution Substances 0.000 description 21
- VYFYYTLLBUKUHU-UHFFFAOYSA-N Dopamine Natural products NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 16
- 150000002500 ions Chemical class 0.000 description 10
- 229960003638 dopamine Drugs 0.000 description 9
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- 241000270722 Crocodylidae Species 0.000 description 3
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 3
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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Abstract
The invention discloses a portable multichannel neurotransmitter detection system, a method and a storage medium, and relates to the field of neurotransmitter detection. The device comprises a signal acquisition module, a channel selection module, an amplification module and a control module; the signal acquisition module is connected with the channel selection module, the channel selection module is connected with the amplification module, and the amplification module is connected with the control module; the signal acquisition module is used for acquiring a probe signal; the channel selection module is used for selecting channels according to the number of the signal acquisition modules; the amplification module is used for amplifying the probe signal to obtain a first signal; and the control module is used for processing the first signal to obtain a detection curve. The invention can detect the information of a plurality of neurotransmitters in one experiment, and the concentration curves of each neurotransmitter can be respectively displayed on the screen in real time, thereby realizing the multichannel neurotransmitter detection and simultaneously considering the portability of the detection equipment.
Description
Technical Field
The invention relates to the technical field of neurotransmitter detection, in particular to a portable multichannel neurotransmitter detection system, a method and a storage medium.
Background
Neural activity is usually accomplished by the coordination of multiple neurotransmitters, and the current primary detection means is a single class of neurotransmitter detection. Ion selective probes are one of the earliest inventive electrochemical sensors. In 1906, Cremer found that two different solutions were separated by a glass membrane, and that a voltage difference existed across the membrane, whose magnitude was related to the hydrogen ion concentration in the solution. After a lot of experiments and researches, Beckman et al finally made a commercial glass electrode-pH glass electrode which can be used for practical measurement. Thereafter, ion electrodes for detecting other cations, such as Na, were developed+Responsive Al-containing2O3Or B2O3Glass electrodes, etc.
Based on the characteristic that most of neurotransmitters are charged in a solution, the detection probe can adopt an ion selective probe, and the ionic strength of a specific neurotransmitter is obtained by measuring a potential. The detection solution is sampled, and then the sampling solution is sent to a special qualified laboratory to be measured by adopting an electrochemical instrument detection system. The electrochemical detection system comprises a personal computer, an electrochemical workstation and a detection probe. The electrochemistry workstation detects the precision height, and measuring method is various, but complete equipment occupation space is big, and detection cost is expensive, detects and needs the reservation, and cycle time is longer, and what frost was more snowy is, and most electrochemistry appearance are single channel information acquisition system, and this means if detect wherein multiple neurotransmitter information in the cerebrospinal fluid, need change corresponding probe in proper order and do many times repeated experiment, and it is very low to throw away detection cost. A few high-end electrochemical instruments can acquire data in double channels, but the expensive price cannot be accepted by common laboratories at all.
Therefore, it is an urgent problem for those skilled in the art to realize multichannel neurotransmitter detection while considering the portability of the detection device.
Disclosure of Invention
In view of the above, the present invention provides a portable multichannel neurotransmitter detection system, method and storage medium, which can realize multichannel neurotransmitter detection and also consider portability of detection equipment.
In order to achieve the purpose, the invention adopts the following technical scheme: on one hand, the portable multichannel neurotransmitter detection system comprises a signal acquisition module, a channel selection module, an amplification module and a control module; the signal acquisition module is connected with the channel selection module, the channel selection module is connected with the amplification module, and the amplification module is connected with the control module;
the signal acquisition module is used for acquiring a probe signal; the channel selection module is used for selecting channels according to the number of the signal acquisition modules; the amplification module is used for amplifying the probe signal to obtain a first signal; and the control module is used for processing the first signal and outputting the change of the concentration of each neurotransmitter in real time according to a Nernst response formula.
Optionally, the system further comprises a communication module, wherein the communication module is connected with the control module and is used for transmitting the probe signal to a terminal device.
Optionally, the detection device further comprises a storage module, wherein the storage module is connected with the control module and used for storing the detection curve.
Optionally, the system further comprises an RFID module, and the RFID module is connected to the control module and is used for identifying the identity of an operator.
Optionally, the signal acquisition module is an ion selective electrode.
Optionally, the detection device further comprises a display module, wherein the display module is connected with the control module and used for displaying the detection curve.
Optionally, the intelligent switch is connected with the control module, the amplification module, the RFID module and the display module, the control module controls the intelligent switch, when the control module inputs a high level, the intelligent switch is turned on to supply power to the amplification module, the RFID module and the display module, and when the control module inputs a low level, the intelligent switch is turned on and off to stop supplying power.
By adopting the technical scheme, the method has the following beneficial technical effects: each module in the whole detection circuit is provided with an intelligent switch, and the intelligent switch is turned on only when in use and is controlled by the control module, so that the power consumption can be reduced.
In another aspect, a portable multichannel neurotransmitter detection method is provided, which comprises the following specific steps:
acquiring probe signals by multiple channels;
amplifying the probe signal to obtain a first signal;
and processing the first signal, and outputting the change of the concentration of each neurotransmitter in real time according to the Nernst response formula.
Optionally, the number of channels is selected according to the number of probe signals to be acquired.
Finally, a computer storage medium is provided, having stored thereon a computer program which, when being executed by a processor, carries out the steps of a portable multichannel neurotransmitter detection method according to the invention.
Compared with the prior art, the invention discloses a portable multichannel neurotransmitter detection system, a method and a storage medium, and has the following beneficial technical effects:
(1) the system integrates the operation and display part into one instrument, simplifies the detection system into two parts, namely a detection instrument and a detection probe, can be carried to the field work by scientific research personnel, realizes the miniaturization and portability of large-scale equipment, and is suitable for various detection environments.
(2) The multi-channel detection is realized, the required neurotransmitter detection probe is simultaneously inserted into the detection solution at one time, each channel can independently detect the corresponding neurotransmitter concentration change, and then the detection curve of each channel is displayed on the screen. The invention simplifies the operation steps, shortens the testing time and reduces the testing cost; the device greatly facilitates relevant detection of different neurotransmitters when researchers carry out animal and medical experiments, and obviously improves the scientific research efficiency.
(3) The access port of each neurotransmitter probe corresponds to a special information acquisition channel, and the information transmission processing is independent and has no interference.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a block diagram of the system of the present invention;
FIG. 2 is a schematic circuit diagram of an amplifier module of the present invention;
FIG. 3 is a schematic circuit diagram of a voltage regulation module of the present invention;
FIG. 4 is a schematic circuit diagram of the positive and negative voltage output modules of the present invention;
FIG. 5 is a schematic diagram of a battery monitoring circuit of the present invention;
FIG. 6(a) is a schematic diagram of a positive voltage intelligent switch circuit of the present invention;
FIG. 6(b) is a schematic diagram of a negative voltage intelligent switch circuit of the present invention;
FIG. 7 is a flow chart of a method of the present invention;
FIG. 8 is a graph comparing the results of the test system and the electrochemical workstation of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment 1 of the invention discloses a portable multichannel neurotransmitter detection system, which comprises a signal acquisition module, a channel selection module, an amplification module and a control module, wherein the signal acquisition module is connected with the channel selection module; the signal acquisition module is connected with the channel selection module, the channel selection module is connected with the amplification module, and the amplification module is connected with the control module;
the signal acquisition module is used for acquiring a probe signal; the channel selection module is used for selecting channels according to the number of the signal acquisition modules; the amplification module is used for amplifying the probe signal to obtain a first signal; and the control module is used for processing the first signal to obtain a detection curve.
Further, the signal acquisition module is an ion selective electrode. For a detection system, the acquisition of the signal is an important part. The amplification module is a circuit for amplifying millivolt-level signals in the collected electrodes in a certain proportion. It requires a certain interference rejection capability while also accurately recovering the original input signal. Since the electrode voltage is measured, the amplification module acts as a voltmeter with amplification effect. To obtain accurate values, an operational amplifier with high impedance needs to be connected in series to eliminate the influence caused by the impedance of the electrode system. The impedance of the electrochemical workstation for laboratory use (CHI660E) was 1012Omega. In the embodiment of the invention, the amplifying circuit adopts a high-impedance chip CA5420A of Intersil company, and the impedance can also reach 1012Omega. The input voltage range was defined as-800 mV to 800mV depending on the voltage range of the acetylcholine and dopamine ion selective electrodes. The input range of the MSP430 analog-to-digital conversion module is 0V to 2.5V, so an amplifying circuit which can amplify 1.5 times and convert a negative voltage input signal into a positive voltage output signal is required to be designedThe schematic diagram of the amplifier module circuit is shown in fig. 2.
In the embodiment, the control module is a single chip microcomputer MSP430F5438A, which is an ultra-low power consumption mixed signal processor with 16-bit computing capability.
In addition, the detection system adopts a 3.7V lithium battery for power supply. During the power supply process, the battery voltage will slowly decrease along with the power loss. In order to reduce the influence of voltage change on the detection circuit and meet the power supply requirements of the single chip microcomputer and the operational amplifier, a voltage stabilizing module is added, as shown in fig. 3. The chip mainly used is TPS79533, which is a low dropout regulator (LDO) capable of converting an input voltage of 3.7V into an output voltage of 3.3V and keeping it stable. The LDO voltage stabilizer is suitable for the condition that the input voltage is close to the output voltage, has high conversion efficiency and reduces the consumption of the power conversion module to the battery.
Also, positive and negative voltage output modules are required, as shown in fig. 4, a reference voltage of 2.5V is generated by ISL60002, and ISL6002 is a very accurate analog voltage reference chip with a deviation within 1 mV. The generated positive voltage of 2.5V is used as the reference voltage of the analog-to-digital conversion module of the singlechip. The positive voltage is converted into a 2.5V negative voltage by the inverter circuit as a reference voltage of the voltage detection circuit.
In order to know the service condition of the battery in real time, a battery monitoring circuit is also required to be designed. As shown in fig. 5, the general method for detecting the battery power is to predict the power consumption of the battery by measuring the voltage of the battery, but the power consumption of the battery is not linear with the voltage, so that the method is not accurate enough. The exact method is coulometry, and the power consumption of the battery is obtained by measuring direct current and time. The DS2780 is a chip designed based on a coulometer method and used for measuring the voltage, the temperature and the current of the lithium battery, estimating the residual capacity and connecting the residual capacity with the control module in a 1-Wire communication mode.
In order to be used for a long time without being connected with an external power supply or being charged, the power consumption needs to be reduced, a chip with low power consumption is selected, and a single chip microcomputer controlled positive and negative voltage intelligent switch is further included, as shown in fig. 6(a) and 6(b), when the single chip microcomputer inputs a high level, the positive and negative voltage intelligent switch is turned on to supply power to each module, and when the low level is input, the switch is turned off to stop supplying power. When all the modules are switched on, the current value is about 102mA, and when the modules are switched off completely, the current value is reduced to 2mA, so that the consumption of the battery is greatly reduced. The electric quantity of the lithium battery used in the embodiment is 1100mAh, the rated working time of the detection system is 10 hours, and the standby time is 23 days.
The communication module is connected with the control module and used for transmitting the probe signal to the terminal equipment. The terminal equipment can be a mobile phone, a computer, wearable equipment and the like. The communication module realizes data transmission with the terminal equipment in a wired and wireless mode.
The device also comprises a storage module, wherein the storage module is connected with the control module and used for storing the detection curve. In this embodiment, the storage module is a Flash memory of samsung corporation.
The RFID module is connected with the control module and used for carrying out identity recognition on an operator.
The device also comprises a display module, wherein the display module is connected with the control module and used for displaying the detection curve. In this embodiment, the display module uses LCD dot matrix screen with backlight, the driving voltage is 3.3V, the resolution is 320 × 240, and RA8806 with its own font library is used to drive the LCD.
The embodiment 2 of the invention discloses a portable multichannel neurotransmitter detection method, which comprises the following specific steps as shown in fig. 7:
acquiring probe signals by multiple channels;
amplifying the probe signal to obtain a first signal;
and processing the first signal to obtain a detection curve.
It should be noted that the number of channels is selected according to the number of probe signals to be acquired.
The ion selective electrode is adopted for signal acquisition, and due to the characteristics of the ion selective electrode, the neurotransmitter electrode needs to be calibrated firstly, and the voltage of the electrode in calibration liquid is used as reference voltage. Firstly, selecting the number of channels according to the number of electrodes, then entering a calibration process of 30 seconds, sampling an input signal by the single chip microcomputer, and starting neurotransmitter detection after sampling is finished. Repeated sampling is not needed in the same experiment, and as long as the electrode calibration is carried out at the beginning, the subsequent detection can skip the calibration link to directly carry out the detection of the neurotransmitter. In the detection process, the detection data is displayed on a screen in real time in a curve mode. After the detection is finished, the data can be stored in Flash, and meanwhile, the data can also be sent to an upper computer for further processing. If the data before the query is needed or the data is not sent in time, the data can be stored firstly, and then the data is sent to the upper computer through the data transmission function.
Specifically, calibration is a necessary step before the use of the ion-selective electrode, and the principle of calibrating the neurotransmitter electrode is as follows: taking the simultaneous detection of acetylcholine and dopamine as an example, 3 electrodes are required, namely an acetylcholine electrode, a dopamine electrode, and a common reference electrode. The voltage of the reference electrode remains constant in any solution. Taking the acetylcholine electrode as an example, the voltage of the acetylcholine electrode will be different in acetylcholine solutions with different concentrations, and different concentrations correspond to different voltages. The calibration function is to select a solution with known acetylcholine concentration as the calibration solution, detect the voltage difference between the acetylcholine electrode and the reference electrode at this time as the standard voltage difference, and then know the acetylcholine concentration corresponding to the voltage difference. After the calibration, the voltage difference obtained by detecting the acetylcholine solution with other concentrations can be compared with the standard voltage difference, and then the current concentration of the acetylcholine solution is obtained.
Furthermore, the concentration of the acetylcholine solution can be obtained according to the voltage difference by the nernst equation, which is as follows:
wherein the voltage difference is EM-E0Value of (E)MIs an acetylcholine electrode voltage, E0For reference electrode voltage, z is the charge number of the ion, F, R, T are indicated separatelyRathz constant, molar gas constant and absolute temperature, aI(aq) is the concentration of the ion I to be detected in the liquid phase.
The process of the invention is illustrated below with reference to specific examples:
cerebrospinal fluid contains various neurotransmitters, such as dopamine, acetylcholine, histamine, etc. This example uses two important neurotransmitter substances in the brain: dopamine and acetylcholine are examples to illustrate how the invention works. The other neurotransmitters can be detected simultaneously in this way.
The dopamine ion selective probe and the acetylcholine ion selective probe are respectively inserted into cerebrospinal fluid, the acetylcholine probe is connected with the green crocodile clip and is connected into a channel 1, the dopamine probe is connected with the red crocodile clip and is connected into a channel 2, and the two probes share one reference electrode and are connected with the black crocodile clip.
At the beginning, the artificial cerebrospinal fluid basal concentration is 10-5M, both curves are in a low level state. Then simulating the brain excitation process, and dripping high-concentration dopamine and acetylcholine solution buffer solution to make the concentration of dopamine and acetylcholine in cerebrospinal fluid quickly reach 10-4And M. It can be seen from the detection curve that the two probes simultaneously exhibit a step response and stabilize after a short period of time. The concentration change of the two can be accurately reflected by the change time.
To test whether the detection system of the present invention has good consistency with an electrochemical workstation. A self-made acetylcholine ion selective probe is used as a detection electrode to detect acetylcholine solutions with different concentrations. First, the electrochemical workstation is used to measure the concentration of 10-5M，10-4M，10-3M，10-2M acetylcholine solutions (background solution PBS buffer) were measured separately, and each concentration was measured 3 times in succession. Similarly, when using the detection system, 10 is used first-6And calibrating the acetylcholine solution of M, and then respectively detecting the acetylcholine solutions with the same concentration, wherein each concentration is detected 3 times. With an electrochemical workstation at 10-5The voltage value measured in the acetylcholine solution of M is used as a reference point for the detection system and the electrochemical workThe results of the station measurements are compared, as shown in FIG. 8, with the abscissa being the voltage level of the electrochemical workstation and the ordinate being the voltage level of the detection system. It can be seen that the data of the two have good linear correlation, which shows that the detection system can accurately acquire the electrode signals and has good consistency with the electrochemical workstation. The reliability of the result is very high, and the field measurement data can be directly used as reference.
Finally, a computer storage medium is provided, on which a computer program is stored which, when being executed by a processor, carries out the steps of a portable multichannel neurotransmitter detection method.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A portable multichannel neurotransmitter detection system is characterized by comprising a signal acquisition module, a channel selection module, an amplification module and a control module; the signal acquisition module is connected with the channel selection module, the channel selection module is connected with the amplification module, and the amplification module is connected with the control module;
the signal acquisition module is used for acquiring a neurotransmitter probe signal; the channel selection module is used for selecting channels according to the number of the signal acquisition modules; the amplification module is used for amplifying the neurotransmitter probe signal to obtain a first signal; and the control module is used for processing the first signal and outputting the change of the concentration of each neurotransmitter in real time according to a Nernst response formula.
2. The portable multichannel neurotransmitter detection system of claim 1, further comprising a communication module coupled to the control module for transmitting the neurotransmitter probe signals to a terminal device.
3. The portable multichannel neurotransmitter detection system of claim 1 further comprising a memory module coupled to the control module for storing neurotransmitter detection results.
4. The portable multichannel neurotransmitter detection system of claim 1 further comprising an RFID module coupled to the control module for identification of the operator.
5. The portable multichannel neurotransmitter detection system of claim 1, wherein the signal acquisition module is an ion-selective electrode.
6. The portable multichannel neurotransmitter detection system of claim 4, further comprising a display module coupled to the control module for displaying the detection result.
7. The system according to claim 6, further comprising an intelligent switch, wherein the intelligent switch is connected to the control module, the amplification module, the RFID module, and the display module, the control module controls the intelligent switch, and when the control module inputs a high level, the intelligent switch is turned on to supply power to the amplification module, the RFID module, and the display module, and when the control module inputs a low level, the intelligent switch is turned on and off to stop supplying power.
8. A portable multichannel neurotransmitter detection method is characterized by comprising the following specific steps:
collecting neurotransmitter probe signals through multiple channels;
amplifying the neurotransmitter probe signal to obtain a first signal;
and processing the first signal, and outputting the change of the concentration of each neurotransmitter in real time according to the Nernst response formula.
9. The portable multichannel neurotransmitter detection method according to claim 8, wherein the number of channels is selected according to the number of neurotransmitter probe signals to be acquired.
10. A computer storage medium, characterized in that the computer storage medium has stored thereon a computer program which, when being executed by a processor, carries out the steps of a portable multichannel neurotransmitter detection method according to any one of the claims 8 to 9.
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Non-Patent Citations (2)
| Title |
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| 何成: "基于全固态离子选择性电极的神经递质检测技术研究", 中国博士学位论文全文数据库, no. 8, 15 August 2017 (2017-08-15), pages 102 * |
| 林楠森;宋轶琳;刘春秀;蔡新霞;: "16通道神经信息双模检测分析仪的研制与应用", 分析化学, no. 05, 15 May 2011 (2011-05-15) * |
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