CN113996942A - Method for fine processing surgical instruments - Google Patents
Method for fine processing surgical instruments Download PDFInfo
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- CN113996942A CN113996942A CN202110538074.0A CN202110538074A CN113996942A CN 113996942 A CN113996942 A CN 113996942A CN 202110538074 A CN202110538074 A CN 202110538074A CN 113996942 A CN113996942 A CN 113996942A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B23—MACHINE TOOLS; METAL-WORKING NOT OTHERWISE PROVIDED FOR
- B23K—SOLDERING OR UNSOLDERING; WELDING; CLADDING OR PLATING BY SOLDERING OR WELDING; CUTTING BY APPLYING HEAT LOCALLY, e.g. FLAME CUTTING; WORKING BY LASER BEAM
- B23K26/00—Working by laser beam, e.g. welding, cutting or boring
- B23K26/36—Removing material
- B23K26/362—Laser etching
- B23K26/364—Laser etching for making a groove or trench, e.g. for scribing a break initiation groove
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Abstract
The invention discloses a method for finely processing a surgical instrument, which comprises the step of forming a strip-shaped groove on the surface of the surgical instrument through laser etching. The method combines laser etching with surgical instruments, and a bacteriostatic surface with bacteriostatic performance is successfully formed on the surgical instruments. The bacteriostatic surface is the change of physical appearance formed on the surface of the surgical instrument, so the bacteriostatic property is independent of any bacteriostatic element, and the bacteriostatic effect is more stable and lasting, safe and effective. In addition, the method can also obviously improve the antibacterial property and easy cleaning property of the surgical instruments.
Description
Technical Field
The invention belongs to the field of medical instruments, and particularly relates to a method for finely processing surgical instruments.
Background
Stainless steel is a widely used base material for medical instruments, and currently, over 80% of stent materials are made of stainless steel. Stainless steel material for existing medical instrumentsThe surface bacteriostasis is mainly realized through the component action: firstly, antibacterial metal elements are integrally added into a stainless steel substrate, and precipitates with antibacterial effect are uniformly dispersed and distributed in the stainless steel through heat treatment; secondly, depositing silver and TiO on the surface of the stainless steel2And the like, so that the surface has antibacterial performance. Although the two methods have certain antibacterial effect, the interaction mechanism between antibacterial metal elements and bacteria is not unified, the precipitation of metal has certain toxicity to human bodies, and in addition, the surface antibacterial film is thin and generally within 100nm, so the metal is easy to wear in the use process, and the antibacterial performance is greatly influenced. The surface of the material treated by the method has no bacteriostatic property, and the surface component elements are used for realizing bacteriostasis, so that once the components are removed, the material has no bacteriostatic property, and thus has no permanent bacteriostatic property.
Therefore, there is currently a need for further improvement in the antibacterial performance of medical devices.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the technical problems in the related art. Therefore, an object of the present invention is to provide a method for fine processing of a surgical instrument, which combines laser etching with a surgical instrument to successfully form a bacteriostatic surface having antibacterial properties on the surgical instrument, wherein the bacteriostatic surface is a change in physical morphology formed on the surface of the surgical instrument, so that the bacteriostatic properties are independent of any bacteriostatic element, and the bacteriostatic effect is more stable, lasting, safe and effective. In addition, the method can also obviously improve the antibacterial property and the easy cleaning property of the surgical operation instrument, improve the cleaning efficiency and reduce the cost.
According to one aspect of the invention, a method of forming a bacteriostatic surface on a surgical implement using laser etching is provided, which, according to a specific embodiment of the invention, includes forming a stripe-shaped groove on the surgical implement by laser etching.
Therefore, the invention combines laser etching with a surgical instrument to manufacture the strip-shaped groove on the surgical instrument, so that the surgical instrument has good performances of hydrophobicity, blood dredging performance, antibacterial adhesion, protein adhesion resistance, blood platelet adhesion resistance and biofilm growth inhibition. Therefore, the surgical instrument has the advantages of easy cleaning and high disinfection efficiency.
In addition, the method for finely processing the surgical instrument according to the above embodiment of the present invention may further have the following additional technical features:
in some embodiments of the present invention, the laser etching uses nanosecond pulse laser, and the laser processing parameters are: the power is 20w, the scanning speed is 600mm/s, the frequency is 20KHz, the scanning times are 10 times, and the scanning interval is 60-150 microns. Thereby effectively etching a trench of a predetermined size in the surgical instrument.
In some embodiments of the present invention, each of the plurality of parallel trenches has a width of 40-60 microns and a depth of 60-70 microns. The formed antibacterial surface has super-hydrophobic property, super-blood dredging property, and good properties of resisting protein adhesion, resisting platelet adhesion and inhibiting the growth of a biological membrane.
In some embodiments of the invention, further comprising: and processing the surface of the microstructure by using a chemical modification method so as to form the bacteriostatic surface. Therefore, the bacteriostatic effect of the bacteriostatic surface can be further improved.
In some embodiments of the invention, the chemical modification method comprises: cleaning the surface of the surgical instrument with absolute ethyl alcohol, soaking the surface of the surgical instrument in 5mmol/L PFDTES methanol solution for 10 hours, placing the surgical instrument in a constant temperature air blast drying oven, solidifying the surgical instrument for 1 hour at the temperature of 150 ℃, taking out the surgical instrument and cooling the surgical instrument.
In some embodiments of the invention, the bacteriostatic surface has superhydrophobic properties and superhydrophobic plasma properties.
In some embodiments of the invention, the bacteriostatic surface has antibacterial adhesion properties, biofilm growth inhibition properties, anti-protein adhesion properties, and anti-platelet adhesion properties.
In some embodiments of the invention, the bacteriostatic ratio of the bacteriostatic surface is not less than 40%.
In some embodiments of the invention, the bacteriostatic surface with the stripe-shaped groove microstructure had an increase in the ability to inhibit escherichia coli adhesion of 39.4%.
Drawings
FIG. 1 is a diagram of a stainless steel sample used in example 1 of the present invention.
Fig. 2 shows a stripe-shaped trench microstructure according to an embodiment of the invention.
FIG. 3 is a three-dimensional pattern of a stripe-shaped trench microstructure etched under conditions of a plurality of scanning pitches and two scanning times in example 1 of the present invention (microscope lens: 10 ×)
Fig. 4 is an SEM image of a stripe-shaped trench microstructure etched using a plurality of scan pitches and 10 scans in example 1 of the present invention.
Fig. 5 is an SEM image of a stripe-shaped trench microstructure etched using a plurality of scan pitches and 20 scans in example 1 of the present invention.
FIG. 6 is a schematic diagram of the measurement of static contact angle and rolling angle in example 2 of the present invention.
FIG. 7 is a graph showing the sterilization vs. cultivation of Escherichia coli adhesion test samples in example 3 of the present invention.
FIG. 8 shows the plate-coating counting results of the E.coli adhesion experiment in example 3 of the present invention.
FIG. 9 is a graph showing the sterilization vs. the slime cultivation of E.coli biofilm test samples in example 4 of the present invention.
FIG. 10 is a confocal laser scanning microscope (1000X) view of example 4 of the present invention.
FIG. 11 is a graph of EDS spectroscopy analysis of sample 5(G) and a control sample (K) in example 5 of the present invention: including 11-G and 11-K.
FIG. 12 is an SEM image of the protein adhesion of sample 5(G) and the control sample (K) in example 5 of the present invention: comprises 12-500X, 12-1000X, 12-2000X, 12-5000X.
FIG. 13 is an optical metalloscope image of protein adhesion on the surface of sample 5(G) and the control sample (K) in example 5 of the present invention.
FIG. 14 is an SEM photograph of platelet adhesion on the surfaces of sample 5(G) and control sample (K) in example 6 of the present invention: comprises 14-1, 14-2 and 14-3.
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative, and is not to be construed as limiting the invention.
According to one aspect of the invention, a method of forming a bacteriostatic surface on a surgical implement using laser etching is provided, which, according to a specific embodiment of the invention, includes forming a stripe-shaped groove on the surgical implement by laser etching. Therefore, the invention combines laser etching with a surgical instrument to manufacture the strip-shaped groove on the surgical instrument, so that the surgical instrument has good performances of hydrophobicity, blood dredging, bacterial adhesion resistance, protein adhesion resistance, platelet adhesion resistance and biofilm growth inhibition. Therefore, the surgical instrument has the advantages of easy cleaning and high disinfection efficiency.
The invention changes the surface microstructure of the surgical operation instrument through laser etching, so that the surface microstructure has bacteriostatic effect, which is a new idea. Specifically, the laser etching technology is adopted, pulse laser acts on the surface of a surgical instrument, a strip-shaped groove shown in figure 2 is processed and prepared on the surface, and the infiltration performance of the surface of the material is changed, so that the effect of reducing or even inhibiting the adhesion of bacteria is realized.
According to the specific embodiment of the invention, the laser etching adopts nanosecond pulse laser. The inventor finds that the microstructure obtained by nanosecond pulse laser etching can achieve the effect of inhibiting or reducing the adhesion of bacteria, and nanosecond pulse laser is more cost-saving than micron laser and has higher etching efficiency.
According to the specific embodiment of the invention, the parameter setting for etching by using nanosecond laser comprises the following steps: the etching power is 20w, the scanning speed is 600mm/s, the frequency is 20KHz, and the scanning times are 10 times. Therefore, under the nanosecond laser etching condition, a groove with a preset size can be effectively etched on the surgical instrument, and the groove has the performance of inhibiting or reducing the adhesion of bacteria. In addition, the inventor finds that the scanning times directly influence the depth of the etched groove, and if the scanning times are too large, the depth of the groove is too deep, and the super-water performance and the super-blood dredging performance of the microstructure are reduced. Further, by optimizing the scanning times, the scanning times are found to be optimal for 10 times, and the depth of the etched groove is 60-70 microns.
According to embodiments of the present invention, the scan pitch may be 60 microns, 90 microns, 120 microns, 150 microns. Therefore, in the strip-shaped grooves obtained by etching under the scanning interval, the interval between the grooves is proper, and the super-hydrophobic and super-blood-dredging performances on the surfaces of the microstructures can be endowed. In addition, the inventor finds out through further research that the strip-shaped groove also has antibacterial adhesion performance, biofilm growth inhibition performance, protein adhesion resistance performance and platelet adhesion resistance performance.
According to the specific embodiment of the invention, the inventor finds that the shape of the microstructure etched by the nanosecond laser directly influences the bacteriostasis effect, and particularly, the bacteriostasis effect of the microstructure with the strip-shaped grooves is optimal.
According to an embodiment of the present invention, if the etched microstructure is a plurality of parallel trenches, the etched dimension may be that the width of each trench in the plurality of parallel trenches is 40-60 microns and the depth is 60-70 microns. The bacteriostatic surface formed by the method has super-hydrophobic property, super-blood dredging property and good properties of resisting protein adhesion, resisting platelet adhesion and inhibiting the growth of a biological membrane. According to a specific example of the present invention, each of the plurality of parallel trenches may have a width of 60 microns, 90 microns, 120 microns, or 150 microns and a depth of 20-50 microns. According to a particular embodiment of the invention, the bacteriostatic ratio of the bacteriostatic surface having the above-mentioned size microstructure is not lower than 40%. In particular, the bacteriostatic surface of the bar-grooved microstructure with the dimensions described above had an improved ability to inhibit adhesion of escherichia coli by 39.4% compared to a surface without any microstructure etched.
According to another embodiment of the present invention, the method of forming a bacteriostatic surface on a surgical implement of the above embodiments further comprises: and carrying out low surface energy modification on the surface of the microstructure by using a chemical modification method, so that the bacteriostatic effect of the bacteriostatic surface can be further improved.
According to a specific embodiment of the present invention, the chemical modification method comprises: washing the surface of the surgical instrument forming the microstructure by using absolute ethyl alcohol, then soaking the surface of the surgical instrument in 5mmol/L PFDTES methanol solution for 10 hours, placing the surgical instrument in a constant-temperature air blast drying oven, solidifying the surgical instrument for 1 hour at the temperature of 150 ℃, taking out the surgical instrument and cooling the surgical instrument. Through chemical modification, the surface of the surgical instrument after laser processing can quickly reach a super-hydrophobic state, and then the antibacterial and blood-dredging performance is quickly realized. In addition, the chemical modification also has the advantages of simple treatment, convenient operation and the like.
Thus, the bacteriostatic surface on the surgical instrument of the above embodiment of the invention has superhydrophobic properties and superhydrophobic plasma properties. Therefore, after the surgical instrument is stained with blood stains, the blood stains can be easily removed by simple alcohol disinfection and cleaning. Thereby significantly improving the cleaning efficiency of the surgical instrument.
In addition, the inventor evaluates the microstructure bacteriostatic surface prepared by the method, and finds that the bacteriostatic surface also has antibacterial adhesion performance, biofilm growth inhibition performance, protein adhesion resistance performance and platelet adhesion resistance performance. Due to the antibacterial adhesion property, the risk of cross infection can be obviously reduced, and the protein adhesion resistance and the platelet adhesion resistance can ensure that tissues, blood and the like are not easy to adhere when the surgical instrument (such as a surgical scalpel) cuts skin or tissues, so that the sharpness and the operation convenience of the surgical instrument can be improved.
Example 1 (preparation of bacteriostatic surface)
(1) Material selection
The martensite 3Cr13 stainless steel adopted in the experiment has higher carbon content, shows higher strength, hardness and wear resistance, and is often used as a material of medical instruments such as surgical cutters and the like. A10 mm by 1mm stainless steel sample (FIG. 1) was used for the experiment, and the composition of the details is shown in Table 1.
TABLE 13 Cr13 composition Table
(2) Laser scanning etching
Firstly, polishing an original processed sample, then immersing the polished sample in absolute ethyl alcohol, ultrasonically oscillating and cleaning for 5 minutes, and drying.
The surface of the substrate is textured by adopting a nanosecond fiber pulse Laser (LJM-50D-III) (the Laser wavelength is 1064nm, the pulse duration is about 100ns, the repetition rate is 20kHz, the focal length is 224mm, the maximum processing range is 110mm multiplied by 110mm, and the diameter of a focus point is 60 mu m), the pretreated sample is irradiated by the Laser, the Laser parameters are adjusted, and the strip-shaped groove structure shown in figure 2 is etched.
The experimental laser processing parameters are as follows: the power is 20w, the scanning speed is 600mm/s, the frequency is 20KHz, the scanning intervals are respectively 60 micrometers, 90 micrometers, 120 micrometers and 150 micrometers, and two samples with the scanning times of 10 times and 20 times are respectively prepared under the corresponding scanning interval conditions. After laser treatment, the mixture is subjected to ultrasonic oscillation cleaning for 10 minutes by using absolute ethyl alcohol and is dried by blowing, and surface impurities and oil stains are removed.
(3) Low surface energy modification
The sample obtained by the above preparation was washed without washing, and then immersed in 5mmol/L methanol solution of PFDTES (1H,1H,2H, 2H-perfluorodecyltriethoxysilane) for 10 hours, and then solidified at 150 ℃ for 1 hour by means of a DH-101-2BS type electrothermal constant temperature air drying oven, and then taken out and left to cool.
(4) Surface topography analysis
The distance between the grooves is increased along with the increase of the scanning distance of the strip-shaped groove structure, and the depth and the width of the groove are basically kept stable; the stripe-shaped trench structure has the advantages that as the scanning times are increased, the depth of the trench is obviously increased under the condition of the same scanning interval, but the width of the trench is not obviously changed, which shows that the scanning times have direct influence on the depth of the trench, as shown in fig. 3-5. As can be seen from fig. 4 and 5 of the scanning electron microscope, in the laser scanning etching, the surface material is instantly melted and sputtered onto the surface of the material due to the irradiation of the high-energy laser, and finally cooled and re-melted at the edges and inside the grooves. The sample after laser processing forms a stable and regular micron-scale array structure.
Example 2 (human plasma wettability test)
(1) The evaluation method comprises the following steps:
static contact angle and rolling angle measurements of water and PRP (platelet rich plasma) were performed on sample 5 prepared in example 1 at a pitch of 90 μm and with a number of scans of 10, and the measurements were averaged three times with a PRP (platelet rich plasma) volume of 10 μ L for contact angle and rolling angle measurements. The assay method is shown in figure 6.
(2) Results and conclusions:
TABLE 2 contact angle and running angle measurement results
As can be seen from Table 2, the water contact angles for samples 3-10 ranged from 147.1 ° to 152.4 °; the water roll angle was in the range of 1 ° -8.5 °, and the PRP contact angle of sample 5 was in the range of 151.9 °; the PRP roll angle is in the range of 7.6 °. It can be shown that sample 5 has superhydrophobic properties and superhydrophobic properties. By comparing the samples scanned 20 times with the samples scanned 10 times, it was found that the samples scanned 10 times had better wetting properties. Therefore, the sample scanned for 10 times has better super-hydrophobic performance, and the processing time can be greatly shortened by 50%, so that the production efficiency is greatly improved. The influence of the scanning distance on the wetting property of the material surface was investigated under the condition that the number of scanning times was 10. The experimental results show that: the wetting property of the material surface is optimal when the scanning distance of the sample 5 is 90 mu m, the static contact angle is 152.4 degrees, and the rolling angle is 1 degree. In addition, the chemical properties of the surface of the super-hydrophobic material are researched. The measurement of the contact angle and the rolling angle of the platelet-rich plasma was performed on the surface of the sample 5 having the best hydrophobic property. The experimental results show that: the static contact angle of plasma on the sample surface was 151.9 ° at a plasma volume of 10 μ L; the roll angle was 7.6 °. Therefore, under the processing conditions of a scanning pitch of 90 μm and a scanning frequency of 10 times, the sample 5 has not only the superhydrophobic property but also the superhydrophobic plasma property. Therefore, under the dual requirements of high efficiency, super-hydrophobic performance and super-blood-dredging performance, the optimal etching condition is considered to be that the scanning distance is 90 micrometers and the scanning times are 10 times. When the scanning distance is too narrow (60 micrometers) or too wide (90-150 micrometers), the super-hydrophobic performance and the super-hemophobic performance of the surface are affected, and the etching efficiency is low due to too many scanning times (20 times).
Example 3 (E.coli adhesion experiment)
(1) The evaluation method comprises the following steps:
test samples: example 1 the resulting sample 5(G) (only the sample 5 experiment was performed) and the control sample (K) which was not laser etched were prepared.
The method comprises the following steps: when carrying out an escherichia coli adhesion experiment, firstly, sterilizing the surface of each sample by using 75% alcohol, naturally drying the sample in an aseptic biological safety cabinet, adhering the dried sample and a culture medium in an opposite manner in order to test the sterilization condition of the surface of the sample, and then putting the adhered culture medium into a constant-temperature biochemical incubator at 37 ℃ for culturing overnight; then respectively putting the samples into a sterile 24-pore plate, adding 1mL of bacterial PBS suspension with OD600 ═ 0.6 into the pore plate filled with the samples, standing for 5 minutes, sucking out the bacterial liquid, and then washing with a sterile PBS solution for three times to wash the non-adhered escherichia coli; and (3) placing the cleaned sample into a centrifuge tube filled with 3mL of sterile PBS solution, carrying out ultrasonic oscillation for 30min, diluting the part of the ultrasonic oscillation solution by 10 times and 100 times in a gradient manner, uniformly coating 100 mu L of the stock solution and the two kinds of ultrasonic oscillation solutions diluted in a gradient manner on a culture dish filled with BHI culture medium, placing the culture dish into a constant-temperature biochemical incubator at 37 ℃ for overnight incubation, and judging the adhesion condition of escherichia coli on the surface of the sample according to the number of bacterial colonies on the culture dish. In order to further observe the residual condition of bacteria on the surface of the sample after ultrasonic oscillation, the oscillated sample is removed and washed with sterile PBS solution for 2 times, then stained with 1% crystal violet solution for 30min, and finally the surface of the sample is observed through a laser confocal microscope and a scanning electron microscope. In order to make the experimental data more accurate, two sets of experiments are set, and the experimental result is the average value of the two sets of experiments.
(2) Results and conclusions:
TABLE 3 results of E.coli adhesion experiments
As shown in FIG. 7, after incubation for 24 hours in a constant-temperature incubator at 37 ℃, neither sample 5 nor the control sample generated colonies on the surface of the sticky culture medium, which can prove that the alcohol sterilization of the sample is thorough before the escherichia coli adhesion experiment, and the surface of the sample is free from contamination of infectious microbes in the experiment process. The results of the adhesion experiments show (fig. 8) that the surface of the sample 5 can effectively reduce the adhesion of escherichia coli, and the bacteriostatic rates of the bar-shaped grooved ultrastructural surface (sample 5) in the stock solution, the 1/10 bacterial solution and the 1/100 bacterial solution are respectively 38.8%, 39.4% and 63.6% through calculation.
Example 4 (E.coli biofilm experiment)
(1) The evaluation method comprises the following steps:
test samples: example 1 the resulting sample 5(G) and a stainless steel control sample (K) that was not laser etched were prepared.
The Escherichia coli biofilm experiment comprises the steps of diluting a cultured bacterial culture solution to OD600 of 0.3, sterilizing and fully drying a sample by using 75% alcohol, then respectively placing the sample into a sterile 24-pore plate, respectively injecting 1mL of diluted bacterial solution into a pore groove containing the sample, then placing the 24-pore plate into a 37 ℃ incubator for incubation for 16 hours, and enabling Escherichia coli to have enough time to grow a biofilm on the sample. After the incubation is finished, sucking out bacterial liquid on the surface of the sample, washing the sample for 3 times by using sterile PBS (phosphate buffer solution), dyeing the sample for 30min by using a 1% crystal violet solution, and finally exciting a dyeing image by using a laser confocal microscope so as to judge the growth condition of the escherichia coli biofilm. In order to make the experimental data more accurate, two sets of experiments were set up.
(2) Results and conclusions:
as shown in FIG. 9, sample 5(G) and control (K) were surface sterilized thoroughly without interference from infectious microbes during the experiment. According to the situation of laser confocal microscope imaging in fig. 10, it can be seen that the fluorescence emitting area of the surface of the sample 5 is significantly lower than that of the surface of the control sample, and in addition, a large area of colonies are generated on the surface of the control sample, so that it can be concluded that more escherichia coli adheres to the surface of the control sample relative to the surface of the strip-shaped groove microstructure. The formation of biofilm is positively correlated with the number of bacteria, and the greater the number of bacteria, the greater the area of biofilm formation. Therefore, it can be seen from the results of confocal laser microscopy that the surface of the striped groove ultrastructure (sample 5) can significantly inhibit the formation of a biofilm.
Example 5 (protein adhesion experiment)
(1) The evaluation method comprises the following steps:
the sample 5(G) and the control sample (K) were placed in a sterile biosafety cabinet and sterilized with 75% ethanol, and then placed in sterile 24-well plates, respectively, and thoroughly dried. 100mg of fibrinogen (Fib) freeze-dried powder is prepared into 5ml of Fib solution with the final concentration of 20 mg/ml. 1mL of the prepared Fib solution was injected into the wells containing the samples, and left at room temperature for 10 min. And then sucking away the fibrin solution on the surface of the sample, washing the sample for 2 times by using a sterile PBS solution, naturally drying the sample, finally judging the composition of substances adhered to the surface by an EDS energy spectrum, and observing the adhesion condition of the fibrin by using a scanning electron microscope and an optical metallographic microscope.
(2) Results and conclusions:
as shown in fig. 11, EDS spectra were performed on the substance adhered to the surface of the bar-shaped groove microstructure (sample 5) and the original surface (control sample), and it was found that the substance adhered to the surface of each sample was protein according to the spectra of the substance adhered to the surface and the atomic percentages of carbon, nitrogen, and oxygen. From the observation results of fig. 12, it can be seen that the fibrin adheres to the surface of the bar-shaped groove microstructure in a filamentous or film form with a small distribution area, and adheres to the original surface in a block or sheet form with a large distribution area. Further observation by an optical metallographic microscope (fig. 13) can more visually see that the surface of the strip-shaped groove microstructure is adhered with a small amount of fibrin compared with the original surface. It can be concluded that the bar-shaped grooved microstructured surface is capable of inhibiting the adhesion of fibrin.
Example 6 (platelet adhesion experiment)
(1) Evaluation method
The spare sample 5(G) and the control sample (K) were placed in a sterile biosafety cabinet and sterilized by 75% alcohol and sufficiently dried. The sterilized samples were placed in wells of a 24-well plate, 1mL each of platelet-rich plasma was dropped into the wells containing the samples, and the plates were incubated in a 37 ℃ incubator for 3 hours. After incubation, sucking redundant plasma in the pore plate by using a pipette gun, washing the plasma for 3 times by using normal saline, and then fixing the plasma for 2 hours at room temperature by using 2.5% glutaraldehyde; the fixed samples were dehydrated sequentially for 20 minutes using 50%, 70% and 100% ethanol/water gradient solutions. The dehydrated sample was dried for 1.5 hours in a critical dryer of CO2, treated with gold spray and observed for platelet morphology and aggregation distortion by Scanning Electron Microscopy (SEM).
(2) Results and conclusions:
as shown in fig. 14, the surface of the stripe-shaped groove microstructure (sample 5) and the original surface (control sample) were photographed and observed by a scanning electron microscope at optional three positions under a field of view of 500 ×. The picture shows that no blood platelet is adhered in the grooves of the strip-shaped groove microstructure surface, and only a small amount of blood platelets are adhered on the protrusions of the microstructure; whereas the original surface had a large number of platelets adhered thereto and some areas had exhibited aggregated adhesion of platelets. Thus, it can be concluded that the bar-shaped grooved microstructure surface (sample 5) can significantly inhibit the adhesion of platelets relative to the original surface (control sample).
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, schematic representations of the above-described terms are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (9)
1. A method of finely processing a surgical instrument, characterized in that a stripe-shaped groove is formed on the surface of the surgical instrument by laser etching.
2. The method according to claim 1, wherein the laser etching uses nanosecond pulsed laser, and the laser processing parameters are as follows: the power is 20w, the scanning speed is 600mm/s, the frequency is 20KHz, the scanning times are 10 times, and the scanning interval is 60-150 microns.
3. The method of claim 1, wherein each of the plurality of parallel trenches has a width of 40-60 microns and a depth of 60-70 microns.
4. The method of claim 2, further comprising: and processing the surface of the microstructure by a chemical modification method so as to form the self-cleaning surface.
5. The method of claim 4, wherein the chemical modification comprises: cleaning the surface of the surgical instrument with absolute ethyl alcohol, soaking the surface of the surgical instrument in 5mmol/L PFDTES methanol solution for 10 hours, placing the surgical instrument in a constant-temperature air-blast drying oven, solidifying the surgical instrument for 1 hour at the temperature of 150 ℃, taking out the surgical instrument and cooling the surgical instrument.
6. The method of claim 1, wherein the bacteriostatic surface has superhydrophobic properties and superhydrophobic plasma properties.
7. The method of claim 1, wherein the bacteriostatic surface has anti-bacterial adhesion properties, biofilm growth inhibition properties, anti-protein adhesion properties, and anti-platelet adhesion properties.
8. The method of claim 1, wherein the bacteriostatic rate of the bacteriostatic surface is not less than 40%.
9. The method of claim 1, wherein the bacteriostatic surface with the striped groove microstructure has an increased ability to inhibit escherichia coli adhesion of at least 39%.
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