CN113984744A - Rice phenols determination method - Google Patents
Rice phenols determination method Download PDFInfo
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- CN113984744A CN113984744A CN202111197565.XA CN202111197565A CN113984744A CN 113984744 A CN113984744 A CN 113984744A CN 202111197565 A CN202111197565 A CN 202111197565A CN 113984744 A CN113984744 A CN 113984744A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 80
- 235000009566 rice Nutrition 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 26
- 150000002989 phenols Chemical class 0.000 title claims abstract description 22
- 240000007594 Oryza sativa Species 0.000 title claims description 6
- 241000209094 Oryza Species 0.000 claims abstract description 74
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000000243 solution Substances 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- OHZCFWMJMWFNFP-ZVGUSBNCSA-L (2r,3r)-2,3-dihydroxybutanedioate;iron(2+) Chemical compound [Fe+2].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O OHZCFWMJMWFNFP-ZVGUSBNCSA-L 0.000 claims abstract description 24
- 229940057006 ferrous tartrate Drugs 0.000 claims abstract description 24
- 239000008055 phosphate buffer solution Substances 0.000 claims abstract description 21
- 229940074391 gallic acid Drugs 0.000 claims abstract description 17
- 235000004515 gallic acid Nutrition 0.000 claims abstract description 17
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims abstract description 16
- LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 claims abstract description 16
- 235000011006 sodium potassium tartrate Nutrition 0.000 claims abstract description 16
- 239000012086 standard solution Substances 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 10
- 229940074439 potassium sodium tartrate Drugs 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims description 20
- 239000000523 sample Substances 0.000 claims description 20
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 20
- 239000012498 ultrapure water Substances 0.000 claims description 20
- 238000002386 leaching Methods 0.000 claims description 17
- 238000007710 freezing Methods 0.000 claims description 11
- 230000008014 freezing Effects 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 238000002835 absorbance Methods 0.000 claims description 9
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 9
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 9
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 9
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 8
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 7
- 239000001488 sodium phosphate Substances 0.000 claims description 7
- 235000011008 sodium phosphates Nutrition 0.000 claims description 7
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 7
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 claims description 6
- 229940048086 sodium pyrophosphate Drugs 0.000 claims description 6
- 235000019818 tetrasodium diphosphate Nutrition 0.000 claims description 6
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 claims description 6
- 241000371652 Curvularia clavata Species 0.000 claims description 5
- 235000007189 Oryza longistaminata Nutrition 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 239000010453 quartz Substances 0.000 claims description 5
- 239000012488 sample solution Substances 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000009472 formulation Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract description 14
- 238000001514 detection method Methods 0.000 abstract description 14
- 239000000126 substance Substances 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract 2
- 238000000691 measurement method Methods 0.000 abstract 1
- 239000001476 sodium potassium tartrate Substances 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 1
- 231100000570 acute poisoning Toxicity 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 208000020470 nervous system symptom Diseases 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
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Abstract
The invention discloses a method for measuring phenols of rice, which relates to the field of phenol measurement, and provides the following scheme aiming at the problems of unsatisfactory measurement effect and slow detection efficiency of the existing partial phenol measurement method, and the method comprises the following steps: step one, putting a sample rice taken back in a field outside for drying in the sun, threshing in the room, and putting the rice in a freezer for storage after shelling; taking out ferrous sulfate heptahydrate and potassium sodium tartrate, and fixing the volume of the ferrous sulfate heptahydrate and the potassium sodium tartrate with water to a volumetric flask to prepare a ferrous tartrate solution which is prepared for use; and step three, taking 0.1ml to 0.5ml of gallic acid standard solution to a 10ml volumetric flask, adding 5ml to 10ml of ferrous tartrate solution, fixing the volume by using phosphate buffer solution with the pH value of 7.5, and shaking up. The method can detect the phenolic substances contained in the rice more quickly, has higher detection efficiency, needs less detection equipment due to simple detection method, has lower detection cost, and is suitable for popularization and use.
Description
Technical Field
The invention relates to the field of phenols determination, in particular to a method for determining phenols of rice.
Background
Phenolic substances belong to high-toxicity substances, when a certain amount of phenolic substances are ingested by a human body, acute poisoning symptoms can appear, water or food polluted by phenols can cause dizziness, eruption, pruritus, anemia and various nervous system symptoms after the rice is drunk for a long time, water sources such as water are polluted by the phenolic substances during the growth process, the rice possibly contains the phenolic substances, the rice is one of staple foods of daily diet of people, so that the detection of the phenolic substances in the rice is necessary, the existing phenolic substance detection is mostly determined by adopting a solution and spectrophotometry method, the phenolic substance determination method is not ideal in determination effect, and the problem of low detection efficiency exists, so that the method for determining the phenols of the rice is provided.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a method for measuring phenols of rice.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for measuring phenols of rice comprises the following steps:
step one, putting a sample rice taken back in a field outside for drying in the sun, threshing in the room, and putting the rice in a freezer for storage after shelling;
taking out ferrous sulfate heptahydrate and potassium sodium tartrate, and fixing the volume of the ferrous sulfate heptahydrate and the potassium sodium tartrate with water to a volumetric flask to prepare a ferrous tartrate solution which is prepared for use;
step three, taking 0.1ml to 0.5ml of gallic acid standard solution to a 10ml volumetric flask, adding 5ml to 10ml of ferrous tartrate solution, fixing the volume with phosphate buffer solution with pH of 7.5, and shaking up;
step four, fully grinding the sample paddy into powder, weighing 0.3-0.6 g of sample powder, adding 12-36 ml of ultrapure water, shaking up, and leaching;
step five, after cooling, filtering, fully washing, fixing the volume to 25-50 ml, and standing for 1 min;
taking out 1ml to 5ml of sample solution to be tested, adding 5ml to 10ml of ferrous tartrate solution, adding phosphate buffer solution to fix the volume to 10ml, shaking up, and standing for 10 min;
and seventhly, after color development, determining the absorbance of the developed color by using a quartz cuvette and clear water as a reference under the condition of 540nm of a UV-752 type ultraviolet spectrophotometer.
Preferably, the airing time of the rice in the step one is controlled to be 7 days to 14 days, the rice is hulled by a huller, the hulled rice is stored in a freezer, and the freezing temperature is controlled to be between-4 ℃ and 6 ℃.
Preferably, the rice in the first step comprises red rice, black rice, glutinous rice, purple rice and white rice, and all the rice is produced in a rice test field.
Preferably, the phosphate buffer solution in the second step includes one or more of sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium phosphate and sodium pyrophosphate, and the phosphate buffer solution provides a formula, by mass, 35% to 50% of sodium dihydrogen phosphate and 50% to 65% of disodium hydrogen phosphate.
Preferably, the ferrous tartrate solution in the second step comprises 50mg to 100mg of ferrous sulfate heptahydrate, 400mg to 500mg of potassium sodium tartrate, and the balance of water, and the total volume is 100 ml.
Preferably, the standard solution of gallic acid in step three is a standard product of gallic acid produced by Solebao solarbio company.
Preferably, the ultrapure water in the fourth step is prepared by using an UlpUPT series ultrapure water purifier.
Preferably, the leaching in the fourth step adopts a water bath mode, the temperature of the water bath is controlled to be 50-70 ℃, the leaching time is controlled to be 1-2 h, and the shaking is required to be carried out every 10-20 min to prevent the precipitation.
Preferably, the filtration time in the fifth step is controlled to be 2min-5min, the filtration times are 5-15 times, the shaking is carried out for 30 seconds-1 minute after the filtration, the constant volume is carried out by adopting ultrapure water, and the standing is carried out after the constant volume is carried out.
Preferably, the solutions are all used by using a dropper, and the shaking time of the step six is controlled between 35 seconds and 1 minute.
Compared with the prior art, the invention has the beneficial effects that: the method has the advantages that the phenolic substances contained in the rice can be detected more quickly, the detection efficiency is higher, in addition, the detection method is simple, the required detection equipment is less, the detection cost is lower, and the problems that the detection effect of the existing partial phenolic determination method is not ideal and the detection efficiency is low are solved.
Drawings
FIG. 1 is a graph showing the standard curve of gallic acid in the present invention.
FIG. 2 is a graph showing a standard curve of absorbance in the present invention.
FIG. 3 is a graph showing an experiment of absorbance of rice in example 1 of the present invention.
FIG. 4 is a graph showing an experiment of absorbance of rice in example 2 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1 to 4, the present invention provides the following technical solutions:
example 1
A method for measuring phenols of rice comprises the following steps:
step one, placing sample rice retrieved from a field outside for drying in the sun, threshing indoors, shelling, and then placing in a freezing cabinet for storage for later use, wherein in the step one, the rice drying time is controlled to be 7 days, shelling is carried out through a sheller, the rice is stored in the freezing cabinet after shelling, the freezing temperature is controlled to be-4 ℃, and the rice in the step one comprises red rice, black rice, glutinous rice, purple rice and white rice which are all produced in a rice test field;
step two, taking out ferrous sulfate heptahydrate and sodium potassium tartrate, diluting the ferrous sulfate heptahydrate and the sodium potassium tartrate with water to a constant volume in a volumetric flask to prepare a ferrous tartrate solution, and using the ferrous tartrate solution as a preparation, wherein a phosphate buffer solution in the step two comprises any one or more of sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium phosphate and sodium pyrophosphate, and the phosphate buffer solution provides a formula according to mass percentage, wherein the sodium dihydrogen phosphate is 35% and the disodium hydrogen phosphate is 65%; the ferrous tartrate solution in the second step comprises 50mg of ferrous sulfate heptahydrate, 400mg of potassium sodium tartrate and the balance of water, and the total volume is 100 ml;
taking 0.1ml of gallic acid standard solution into a 10ml volumetric flask, adding 5ml of ferrous tartrate solution, fixing the volume by using a phosphate buffer solution with the pH value of 7.5, and shaking up, wherein the gallic acid standard solution in the third step adopts a gallic acid standard product produced by Solebao solarbio company;
step four, fully grinding sample paddy into powder, weighing 0.3g of sample powder, adding 12ml of ultrapure water, shaking up, and leaching, wherein the ultrapure water in the step four is prepared by a UpUPT series ultrapure water purifier, the leaching in the step four adopts a water bath mode, the water bath temperature is controlled at 50 ℃, the leaching time is controlled at 1h, and the sample paddy is required to shake once every 10min to prevent precipitation;
step five, after cooling, filtering, fully washing, fixing the volume to 25ml, standing for 1min, controlling the filtering time in the step five to be 2min, controlling the filtering times to be 5 times, shaking for 30 seconds after filtering, fixing the volume by adopting ultrapure water, and standing after fixing the volume;
taking out 1ml of sample solution to be detected, adding 5ml of ferrous tartrate solution, adding phosphate buffer solution to fix the volume to 10ml, shaking up, standing for 10min, and controlling the shaking time of the step six to be 35 seconds;
and seventhly, determining the absorbance of the developed product by using a quartz cuvette and clear water as a reference under the condition of 540nm of a UV-752 type ultraviolet spectrophotometer, wherein the specific numerical value refers to fig. 3.
Example 2
A method for measuring phenols of rice comprises the following steps:
step one, placing sample rice retrieved from a field outside for drying in the sun, threshing indoors, shelling, then placing the rice in a freezing cabinet for storage for later use, wherein in the step one, the rice drying time is controlled to be 14 days, shelling is carried out through a sheller, the rice is stored in the freezing cabinet after shelling, the freezing temperature is controlled to be 6 ℃, and the rice in the step one comprises red rice, black rice, glutinous rice, purple rice and white rice which are all produced in a rice test field;
taking out ferrous sulfate heptahydrate and sodium potassium tartrate to a volumetric flask with water to fix the volume to prepare a ferrous tartrate solution, and preparing the ferrous tartrate solution for use, wherein the phosphate buffer solution in the second step comprises one or more of sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium phosphate and sodium pyrophosphate, the phosphate buffer solution provides a formula according to mass percentage, the sodium phosphate is 50%, and the sodium pyrophosphate is 50%, the ferrous tartrate solution in the second step comprises 100mg of ferrous sulfate heptahydrate, 500mg of sodium potassium tartrate and the balance of water, and the total volume is 100 ml;
taking 0.5ml of gallic acid standard solution into a 10ml volumetric flask, adding 10ml of ferrous tartrate solution, fixing the volume by using a phosphate buffer solution with the pH value of 7.5, and shaking up, wherein the gallic acid standard solution in the step three adopts a gallic acid standard product produced by Solebao solarbio company;
step four, fully grinding sample paddy into powder, weighing 0.6g of sample powder, adding 36ml of ultrapure water, shaking up, and leaching, wherein the ultrapure water in the step four is prepared by a UpUPT series ultrapure water purifier, the leaching in the step four adopts a water bath mode, the water bath temperature is controlled at 70 ℃, the leaching time is controlled at 2h, and the sample paddy is required to shake once every 20min to prevent precipitation;
step five, after cooling, filtering, fully washing, fixing the volume to 50ml, standing for 1min, controlling the filtering time in the step five to be 5min, controlling the filtering times to be 15 times, shaking for 1min after filtering, fixing the volume by adopting ultrapure water, and standing after fixing the volume;
taking out 5ml of sample solution to be detected, adding 10ml of ferrous tartrate solution, adding phosphate buffer solution to fix the volume to 10ml, shaking up, standing for 10min, and controlling the shaking time of the step six to be 1 min;
and seventhly, after color development, determining the absorbance of the developed color by using a quartz cuvette and clear water as a reference under the condition of 540nm of a UV-752 type ultraviolet spectrophotometer, wherein the specific numerical value refers to fig. 4.
Example 3
The method for measuring phenols of rice comprises the following steps:
step one, placing sample rice taken back in a field outside for drying in the sun, threshing in the room, shelling, then placing in a freezing cabinet for storage for later use, wherein in the step one, the rice drying time is controlled to be 7-14 days, shelling is carried out by a sheller, the rice is stored in the freezing cabinet after shelling, the freezing temperature is controlled to be-4-6 ℃, and the rice in the step one comprises red rice, black rice, glutinous rice, purple rice and white rice which are all produced in a rice test field;
taking out ferrous sulfate heptahydrate and sodium potassium tartrate, and fixing the volume of the ferrous sulfate heptahydrate and the sodium potassium tartrate with water to a volumetric flask to prepare a ferrous tartrate solution for use, wherein the phosphate buffer solution in the second step comprises one or more of sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium phosphate and sodium pyrophosphate, the phosphate buffer solution provides a formula according to mass percentage, the sodium dihydrogen phosphate is 35% and the sodium phosphate is 65%, the ferrous tartrate solution in the second step comprises 70mg of ferrous sulfate heptahydrate, 450mg of sodium potassium tartrate and the balance of water, and the total volume is 100 ml;
step three, taking 0.4ml of gallic acid standard solution into a 10ml volumetric flask, adding 7ml of ferrous tartrate solution, fixing the volume by using a phosphate buffer solution with the pH value of 7.5, and shaking up, wherein the gallic acid standard solution in the step three adopts a gallic acid standard product produced by Solebaolarbio company;
step four, fully grinding sample paddy into powder, weighing 0.5g of sample powder, adding 30ml of ultrapure water, shaking up, and leaching, wherein the ultrapure water in the step four is prepared by a UpUPT series ultrapure water purifier, the leaching in the step four adopts a water bath mode, the water bath temperature is controlled at 60 ℃, the leaching time is controlled at 1.5h, and the sample paddy is required to shake once every 15min to prevent precipitation;
step five, after cooling, filtering, fully washing, fixing the volume to 35ml, standing for 1min, controlling the filtering time in the step five to be 3min, controlling the filtering times to be 10 times, shaking for 45 seconds after filtering, fixing the volume by adopting ultrapure water, and standing after fixing the volume;
taking out 4ml of sample solution to be detected, adding 6ml of ferrous tartrate solution, adding phosphate buffer solution to fix the volume to 10ml, shaking uniformly, and standing for 10min, wherein the shaking time in the sixth step is controlled to be 45 seconds;
and seventhly, after color development, determining the absorbance of the developed color by using a quartz cuvette and clear water as a reference under the condition of 540nm of a UV-752 type ultraviolet spectrophotometer.
Single experiment:
the quality is controlled, single-factor tests under different temperature conditions are carried out under the condition that the water bath leaching time is the same, through the fluctuation of the absorbance value, the solution is relatively stable under the condition of 60 ℃, the error of the experimental result is smaller, but through the comparison and analysis of the average value, under the condition of 60 ℃, the solution is not the optimal experimental condition, as can be seen from the following table, two peak values exist between 70 ℃ and 50 ℃, so the water bath leaching sample under the condition of 60 ℃ is selected, and the measured phenol content is lower. Thus, at the same level, a range of high and low levels of phenolic content measured does not reflect the maximum assay value of the test sample, as shown in the table below:
the above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A method for measuring phenols of rice is characterized by comprising the following steps:
step one, putting a sample rice taken back in a field outside for drying in the sun, threshing in the room, and putting the rice in a freezer for storage after shelling;
taking out ferrous sulfate heptahydrate and potassium sodium tartrate, and fixing the volume of the ferrous sulfate heptahydrate and the potassium sodium tartrate with water to a volumetric flask to prepare a ferrous tartrate solution which is prepared for use;
taking 0.1ml to 0.5ml of gallic acid standard solution into a 10ml volumetric flask, adding 5ml of ferrous tartrate solution, fixing the volume by using a phosphate buffer solution with the pH value of 7.5, and shaking up;
step four, fully grinding the sample paddy into powder, weighing 0.3-0.6 g of sample powder, adding 12-36 ml of ultrapure water, shaking up, and leaching;
step five, after cooling, filtering, fully washing, fixing the volume to 25-50 ml, and standing for 1 min;
taking out 1ml to 5ml of sample solution to be tested, adding 5ml to 10ml of ferrous tartrate solution, adding phosphate buffer solution to fix the volume to 10ml, shaking up, and standing for 10 min;
and seventhly, after color development, determining the absorbance of the developed color by using a quartz cuvette and clear water as a reference under the condition of 540nm of a UV-752 type ultraviolet spectrophotometer.
2. The method for measuring phenols of rice as claimed in claim 1, wherein the drying time of rice in the first step is controlled to 7 days to 14 days, and the rice is hulled by a huller, and then stored in a freezer after hulling, and the freezing temperature is controlled to-4 ℃ to 6 ℃.
3. The method for measuring phenols of rice as claimed in claim 1, wherein the rice in the first step includes red rice, black rice, glutinous rice, purple rice, and white rice, and all of them are produced in rice test field.
4. The method for determining phenols of rice as claimed in claim 1, wherein the phosphate buffer solution in the second step comprises one or more of sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium phosphate, and sodium pyrophosphate, and the phosphate buffer solution provides a formulation by mass percentage of 35% -50% of sodium dihydrogen phosphate and 50% -65% of disodium hydrogen phosphate.
5. The method for assaying phenols of rice as claimed in claim 1, wherein the ferrous tartrate solution in the second step contains 50mg to 100mg of ferrous sulfate heptahydrate, 400mg to 500mg of potassium sodium tartrate, and the balance of water, and has a total volume of 100 ml.
6. The method for assaying phenols of rice according to claim 1, wherein the standard solution of gallic acid used in step three is a standard gallic acid produced by Solebao solarbio Co.
7. The method for measuring phenols of rice as claimed in claim 1, wherein the ultrapure water in the fourth step is prepared by using a UpUPT series ultrapure water purifier.
8. The method for determining phenols of rice as claimed in claim 1, wherein the leaching in the fourth step is performed by means of water bath, the temperature of the water bath is controlled to be 50-70 ℃, the leaching time is controlled to be 1-2 h, and the shaking is performed every 10-20 min to prevent precipitation.
9. The rice phenols determination method as claimed in claim 1, wherein the filtration time in the fifth step is controlled to be 2min-5min, the filtration frequency is controlled to be 5 times-15 times, the fifth step is performed by shaking for 30 seconds-1 minute after filtration, the fifth step is performed by using ultrapure water for constant volume, and the fifth step is performed by standing after constant volume.
10. A method for measuring phenols of rice as claimed in claim 1, wherein the solution is used by a dropper, and the shaking time in the sixth step is controlled to be 35 seconds to 1 minute.
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