CN113984734A - Background subtraction detection method and system for Raman spectrum and Raman spectrometer - Google Patents
Background subtraction detection method and system for Raman spectrum and Raman spectrometer Download PDFInfo
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Abstract
The invention relates to a background subtraction detection method and system of a Raman spectrum and a Raman spectrometer, which sequentially acquire a first time delay delta t after each pulse laser emission according to a time sequence under a preset condition1To Δ t1(ii) Raman scattered light during + w time periods, and simultaneously acquiring a second time delay Deltat after each emission of pulsed laser light2To Δ t2The background light of the detection background in the + w time period can realize quasi-synchronous detection of the Raman spectrum of the sample to be detected and the background spectrum of the detection background, the background spectrum of the detection background is deducted from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected, error deduction can be avoided, the acquisition and deduction of the Raman spectrum of the sample to be detected and the background spectrum of the detection background under a complex and rapidly-changing light environment can be met, and the real Raman spectrum of the sample to be detected can be obtained by obtaining the final Raman spectrum of the sample to be detected.
Description
Technical Field
The invention relates to the technical field of Raman spectrum detection, in particular to a background subtraction detection method and system for Raman spectrum and a Raman spectrometer.
Background
The signal of Raman scattered light is very weak and is 10 of Rayleigh scattering-6～10-9Magnitude. When a raman spectrometer is used for raman spectrum acquisition of a target substance, a very sensitive detector is required to acquire a weak signal, interference signals in various detection backgrounds, such as fluorescence background, ambient light background, detector noise, rayleigh scattering of laser and the like, are also included in the obtained raman spectrum containing the target substance, the intensity of part of the interference signals is very strong, and under normal conditions, due to the consideration of sensitivity, the interference cannot be completely eliminated by the improvement of the equipment performance of the raman spectrometer. The elimination of ambient light background and detector noise is usually achieved by strictly shading the detection sampling device and the sample, turning off the laser, collecting one or more sets of background signals under the same detection conditions, and subtracting the background signals from the material signals. Because the raman signal is weak, the time for this signal and background noise acquisition varies from seconds to tens of seconds.
The method is effective for Raman spectrum application with good light-shielding condition and weak and stable background light, the background signal acquired in the same time period can be approximately equal to the background signal acquired in the Raman signal acquisition process, but for the application that part of the Raman spectrum can not shield non-Raman scattering background light through a physical method and the background light change frequency is extremely fast, such as the Raman spectrum in-situ online test of the state of a catalyst in photo-thermal catalytic reaction, and the combustion Raman spectrum detection experiment process can generate a strong and extremely unstable light background environment; for example, long-distance Raman spectrum detection has long detection optical path and complex and uncontrollable ambient light background. At this time, if the conventional background collection and subtraction method is used, the light background environment has changed greatly within a time period of several seconds or even tens of seconds, and the interference of the background light cannot be removed by the subtraction method, that is, in the conventional background subtraction method, because the single collection time is long, the time interval is long, and the false subtraction can be caused for the rapidly changing and unstable light background.
Disclosure of Invention
The invention provides a background subtraction detection method and system of Raman spectrum and a Raman spectrometer, aiming at the defects of the prior art.
The technical scheme of the background subtraction detection method of the Raman spectrum is as follows:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser emission according to the time sequence1To Δ t1Generating a Raman spectrum of the sample to be detected by Raman scattering light in + w time period, and simultaneously collecting a second time delay delta t after each pulse laser emission2To Δ t2Detecting background light of a background in a + w period, and generating a background spectrum of the detected background, wherein w is the pulse width of pulse laser emitted by a laser in the Raman spectrometer;
and deducting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected.
The background subtraction detection method of the Raman spectrum has the following beneficial effects:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser emission according to the time sequence1To Δ t1(ii) Raman scattered light during + w time periods, and simultaneously acquiring a second time delay Deltat after each emission of pulsed laser light2To Δ t2The background light of the detection background in the + w time period can realize quasi-synchronous detection of the Raman spectrum of the sample to be detected and the background spectrum of the detection background, the background spectrum of the detection background is deducted from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected, error deduction can be avoided, the acquisition and deduction of the Raman spectrum of the sample to be detected and the background spectrum of the detection background under a complex and rapidly-changing light environment can be met, and the real Raman spectrum of the sample to be detected can be obtained by obtaining the final Raman spectrum of the sample to be detected.
On the basis of the above scheme, the method for background subtraction detection of raman spectrum of the present invention can be further improved as follows.
Further, the obtaining of the first time delay includes:
calculating a first time delay deltat according to a first formula1The first formula is:
wherein, L is the distance between a sampling probe of the Raman spectrometer and the sample to be measured, and c is the light speed.
Further, the obtaining of the second time delay includes:
calculating a second time delay deltat according to a second formula2The second formula is: Δ t2＝Δt1+ w + Δ, where Δ is a preset duration, and Δ ranges from 0 to w.
The technical scheme of the background subtraction detection system of the Raman spectrum is as follows:
the acquisition generation module is used for:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser emission according to the time sequence1To Δ t1Generating a Raman spectrum of the sample to be detected by Raman scattering light in + w time period, and simultaneously collecting a second time delay delta t after each pulse laser emission2To Δ t2Detecting background light of a background in a + w period, and generating a background spectrum of the detected background, wherein w is the pulse width of pulse laser emitted by a laser in the Raman spectrometer;
the deduction module is configured to: and deducting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected.
The background subtraction detection system of the Raman spectrum has the following beneficial effects:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser emission according to the time sequence1To Δ t1(ii) Raman scattered light during + w time periods, and simultaneous acquisition after each emission of pulsed laser lightSecond time delay deltat2To Δ t2The background light of the detection background in the + w time period can realize quasi-synchronous detection of the Raman spectrum of the sample to be detected and the background spectrum of the detection background, the background spectrum of the detection background is deducted from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected, error deduction can be avoided, the acquisition and deduction of the Raman spectrum of the sample to be detected and the background spectrum of the detection background under a complex and rapidly-changing light environment can be met, and the real Raman spectrum of the sample to be detected can be obtained by obtaining the final Raman spectrum of the sample to be detected.
On the basis of the above scheme, the background subtraction detection system of raman spectrum of the present invention can be further improved as follows.
Further, the system also comprises an acquisition module, wherein the acquisition module is used for:
calculating a first time delay deltat according to a first formula1The first formula is:
wherein, L is the distance between a sampling probe of the Raman spectrometer and the sample to be measured, and c is the light speed.
Further, the obtaining module is further configured to:
calculating a second time delay deltat according to a second formula2The second formula is: Δ t2＝Δt1+ w + Δ, where Δ is a preset duration, and Δ ranges from 0 to w.
The technical scheme of the Raman spectrometer is as follows:
comprising a control chip for performing a method of background subtraction detection of raman spectra as described in any one of the above.
Drawings
FIG. 1 is a schematic flow chart of a background subtraction detection method for Raman spectroscopy according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a Raman spectrometer;
FIG. 3 is a timing diagram of the timing of the laser pulses, the acquisition timing of the Raman signal of the photodetector, and the acquisition timing of the background signal of the photodetector;
FIG. 4 is a Raman spectrum of a sample to be measured;
FIG. 5 is a detection background spectrum;
FIG. 6 is a Raman spectrum of a sample to be measured and an average spectrum accumulated by multiple measurements of a probing background;
FIG. 7 is the final Raman spectrum of the sample to be measured;
FIG. 8 is a schematic diagram of a Raman spectroscopy background subtraction detection system according to an embodiment of the present invention;
Detailed Description
As shown in fig. 1, a method for background subtraction detection of raman spectrum according to an embodiment of the present invention includes the following steps:
s1, sequentially collecting the first time delay delta t after each pulse laser emission according to the time sequence under the preset condition1To Δ t1Generating a Raman spectrum of the sample to be detected by Raman scattering light in + w time period, and simultaneously collecting a second time delay delta t after each pulse laser emission2To Δ t2Detecting background light of a background in a + w period, and generating a background spectrum of the detected background, wherein w is the pulse width of pulse laser emitted by a laser in the Raman spectrometer;
and S2, deducting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected.
Specifically, S1 and S2 are realized by a raman spectrometer, as shown in fig. 2, the raman spectrometer includes: the device comprises a laser 1, a Raman spectrum probe, namely a sampling probe 2, a spectrum analyzer 4 and a photoelectric detector 3, wherein the laser 1 can be a pulse laser 1, such as a picosecond pulse laser, a nanosecond pulse laser, a microsecond pulse laser and the like; the photodetector 3 can be a time gating photodetector 3, specifically, the photodetector 3 with the same level of time gating precision as the pulse laser is selected for use, is used for detecting Raman spectrum signals and background signals synchronously with the pulse laser in the time domain, and can be a photomultiplier, an ICCD with an image intensifier, an ICMOS with an image intensifier, or the like. The specific detection principle is as follows:
the laser device 1 is used for emitting pulse laser for exciting a sample 5 to be detected to the sample 5 to be detected, the sample 5 to be detected generates Raman scattering light under the excitation of the pulse laser, the photoelectric detector 3 collects the Raman scattering light of the sample 5 to be detected and sends the Raman scattering light to the spectrum analyzer 4, and the spectrum analyzer 4 is used for decomposing the polychromatic Raman scattering light into monochromatic spectrum signals and analyzing the monochromatic spectrum signals, so that the Raman spectrum of the sample 5 to be detected is obtained. The raman detection process is well known to those skilled in the art and will not be described herein.
A first time delay deltat after each emission of pulsed laser light is acquired in sequence1To Δ t1While collecting Raman scattered light in + w period, collecting second time delay delta t after each pulse laser emission2To Δ t2Background light of the detection background of + w period, wherein the detection background means: sequentially collecting the first time delay delta t after each pulse laser emission1To Δ t1The environment background where the raman scattering light of + w period is located detects that there is background light in the background, and the photodetector 3 also receives the background light in the detection environment to generate a background spectrum of the detection background.
The process of obtaining the raman spectrum of the sample 5 to be measured in S1 is explained as follows: for example, the frequency of the emitted pulsed laser light is 10 times per second, the pulse width w is 10ns, and the first time delay Δ t1100 ns; then:
at the time of 0, the pulse laser is emitted for the first time, and the first time delay delta t after the pulse laser is emitted for the first time is collected1To Δ t1Raman scattered light S in + w period, i.e. 100ns to 110ns period1；
At 0.1 second, the pulse laser is emitted for the second time, and the first time delay delta t is acquired after the pulse laser is emitted for the second time1To Δ t1Raman scattered light S in + w time interval, i.e. 0.1S +100ns to 0.1S +110ns time interval2；
When the pulse laser is emitted for the third time at 0.2 second, the first time delay delta t is acquired after the pulse laser is emitted for the third time1To Δ t1Raman scattered light S in + w time interval, namely 0.2S +100ns to 0.2S +110ns time interval3；
By analogy, the first time delay delta t after each pulse laser emission is collected1To Δ t1And (5) obtaining the Raman spectrum of the sample 5 to be detected by the Raman scattering light in the + w time period, wherein the preset conditions are as follows: presetting time length, a first preset time threshold value for collecting Raman scattering light, or a second preset time threshold value for emitting pulse laser;
in practical operation, the gate width W is used as the laser light pulse width of the laser 1, and is usually in the order of ps to ns; initializing the target Raman signal intensity S to be 0, and detecting the cumulative number i to be 1; a single pulse laser with a pulse width W is emitted, and the spectrum analyzer 43 collects a single pulse excitation Raman spectrum signal S with a gate width W and a time delay of Δ tiWhen the number of times of collecting the Raman scattering light reaches a first preset number threshold SthAnd when the Raman signal acquisition of the sample 5 to be detected is finished, otherwise, the laser pulse is continuously emitted and the acquisition is continued. Thus, the raman spectrum of the sample 5 to be measured is obtained as follows: s0 ═ Σ Si；
The process of obtaining the background spectrum of the detection background in S2 is explained as follows:
for example, the frequency of the emitted pulsed laser light is 10 times per second, the pulse width w is 10ns, and the first time delay Δ t1100ns, Δ w 10ns, then Δ t2＝120ns：
At the time of 0, the pulse laser is emitted for the first time, and the second time delay delta t after the pulse laser is emitted for the first time is collected2To Δ t2Background light of the detection background in + w period, namely 120ns to 130ns period;
at 0.1 second, the pulse laser is emitted for the second time, and the first time delay delta t is acquired after the pulse laser is emitted for the second time2To Δ t2Background light of a detection background in a + w period, namely a period of 0.1s +120ns to 0.1s +130 ns;
when the pulse laser is emitted for the third time at 0.2 second, the first time delay delta t is acquired after the pulse laser is emitted for the third time2To Δ t2Background light of a detection background in a + w period, namely 0.2s +120ns to 0.2s +130ns period;
by analogy, collect each time of the hairSecond time delay deltat after pulse laser2To Δ t2Background light of a detection background for a + w period, generating a background spectrum of the detection background
Wherein the first time-delayed acquisition process comprises:
calculating a first time delay deltat according to a first formula1The first formula is:
wherein, L is the distance between the sampling probe 2 of the Raman spectrometer and the sample 5 to be measured, and c is the speed of light.
Wherein, the collection frequency f for collecting the Raman scattering light of the sample 5 to be measured and the width w of the collection time window are both consistent with the excitation light.
Wherein the obtaining of the second time delay comprises:
calculating a second time delay deltat according to a second formula2The second formula is: Δ t2＝Δt1+ w + Δ, where w is a pulse width of a pulse laser emitted by the laser 1 in the raman spectrometer, Δ is a preset duration, and a value range of Δ is 0-w.
The acquisition frequency f of the Raman scattering light of the acquisition detection background and the width w of the acquisition time window are consistent with the excitation light.
Setting total collection time as t, collecting total Raman scattering light of the sample 5 to be detected for N ═ t × f times, and collecting total Raman scattering light of the detection background for N ═ t × f times, accumulating the Raman scattering light of the sample 5 to be detected for N times to obtain a Raman spectrum S0 of the sample 5 to be detected,
Sithe ith Raman scattering light of the sample 5 to be detected is shown, the N times of background light of the detection background is accumulated to obtain a detection background spectrum B0,
Bithe ith background light representing the detection background is removed by using the formula Sr-S0-B0And measuring the background spectrum to obtain the final Raman spectrum Sr of the sample 5 to be measured.
The time sequence of the laser pulse, the acquisition time sequence of the raman signal of the photodetector 3, and the acquisition time sequence of the background signal of the photodetector 3 are shown in fig. 3, specifically:
1)1 is the time sequence of the pulse laser, the pulse width of the pulse laser is w, and the frequency is f;
2)2, the acquisition time sequence of the Raman signal of the photoelectric detector 3 is shown, the acquisition gate width is w, the frequency is f, and the delay relative to the laser pulse is delta t 0;
3) and 3, the acquisition time sequence of the background signal of the photoelectric detector 3 is shown, the acquisition gate width is w, the frequency is f, and the delay relative to the acquisition time sequence of the Raman signal is w + delta.
The following description of the technical effect of the background subtraction detection method for raman spectroscopy in the application by using a remote laser raman spectrometer to detect a sample 5 to be detected at a distance of 100 meters is provided, specifically:
detecting the distance: l is 100 m;
pulse width of the laser 1: w is 10 ns;
excitation light frequency: f is 5 Hz;
the buffering time delta w is 10 ns;
detection time: t is 3 s;
a first time delay: Δ t1＝667ns；
A second time delay: Δ t2＝687ns；
Light background: natural light background, lighting LED light source, and flashing frequency of the commercial power alternating current frequency of 2 times, namely 100 Hz.
In the acquisition time of 3s, the remote laser raman spectrometer acquires 15 groups of raman scattering light of the sample 5 to be detected and 15 groups of detection background light, the acquired signal background has certain difference due to the tiny fluctuation of the natural background light in 3s and the stroboscopic fluctuation of the LED lamp, the time span of the raman scattering light and the detection background light of the sample 5 to be detected in the same group is only 30ns, the light background can be considered to be relatively constant in such a short time, and the light background noise can be subtracted in a quasi-real-time manner by the subtraction of the raman spectrum.
The raman spectrum of the sample 5 to be measured is shown in fig. 4, the detection background spectrum is shown in fig. 5, the average curve of the raman spectrum of the sample 5 to be measured and the detection background spectrum is shown in fig. 6, and the final raman spectrum of the sample 5 to be measured is shown in fig. 7.
As can be seen from fig. 4 and 5, the light background acquired by multiple acquisitions has a certain fluctuation, resulting in the same fluctuation of the acquired background spectrum; as can be seen from fig. 3 and 4, the light background subtracted by the present invention is good, and the influence of the fluctuation of the background can be completely eliminated.
According to the background subtraction detection method of Raman spectrum, under the preset condition, according to the time sequence, the first time delay delta t after each pulse laser emission is collected in sequence1To Δ t1(ii) Raman scattered light during + w time periods, and simultaneously acquiring a second time delay Deltat after each emission of pulsed laser light2To Δ t2The background light of the detection background in the + w time period can realize quasi-synchronous detection of the Raman spectrum and the detection background spectrum of the sample 5 to be detected, the detection background spectrum is deducted from the Raman spectrum of the sample 5 to be detected to obtain the final Raman spectrum of the sample 5 to be detected, error deduction can be avoided, the acquisition and deduction of the Raman spectrum and the detection background spectrum of the sample 5 to be detected in a complex and rapidly-changing light environment can be met, and the real Raman spectrum of the sample 5 to be detected can be obtained by obtaining the final Raman spectrum of the sample 5 to be detected.
In the above embodiments, although the steps are numbered as S1, S2, etc., but only the specific embodiments are given in this application, and those skilled in the art may adjust the execution sequence of S1, S2, etc. according to the actual situation, which is also within the protection scope of the present invention, it is understood that some embodiments may include some or all of the above embodiments.
As shown in fig. 8, a background subtraction detection system 200 for raman spectroscopy according to an embodiment of the present invention includes an acquisition generation module 210 and a subtraction module 220;
the acquisition generation module 210 is configured to:
under the condition of the presetting condition, the air conditioner is controlled to be in a closed state,according to the time sequence, the first time delay delta t after each pulse laser emission is collected in sequence1To Δ t1Generating a Raman spectrum of the sample to be detected by Raman scattering light in + w time period, and simultaneously collecting a second time delay delta t after each pulse laser emission2To Δ t2Detecting background light of a background in a + w period, and generating a background spectrum of the detected background, wherein w is the pulse width of pulse laser emitted by a laser in the Raman spectrometer;
the deduction module 220 is configured to: and deducting the Raman spectrum of the detection background from the Raman spectrum of the sample 5 to be detected to obtain the final Raman spectrum of the sample 5 to be detected.
Under the preset condition, sequentially collecting the first time delay delta t after each pulse laser emission according to the time sequence1To Δ t1(ii) Raman scattered light during + w time periods, and simultaneously acquiring a second time delay Deltat after each emission of pulsed laser light2To Δ t2The background light of the detection background in the + w time period can realize quasi-synchronous detection of the Raman spectrum of the sample 5 to be detected and the Raman spectrum of the detection background, the Raman spectrum of the detection background is deducted from the Raman spectrum of the sample 5 to be detected to obtain the final Raman spectrum of the sample 5 to be detected, error deduction can be avoided, the acquisition and deduction of the Raman spectrum of the sample 5 to be detected and the Raman spectrum of the detection background under a complex and rapidly-changing light environment can be met, and the real Raman spectrum of the sample 5 to be detected can be obtained by obtaining the final Raman spectrum of the sample 5 to be detected.
Preferably, in the above technical solution, the apparatus further includes an obtaining module, where the obtaining module is configured to:
calculating a first time delay deltat according to a first formula1The first formula is:
wherein, L is the distance between the sampling probe 2 of the Raman spectrometer and the sample 5 to be measured, and c is the speed of light.
Preferably, in the above technical solution, the obtaining module is further configured to:
according to a second formulaCalculating a second time delay Deltat2The second formula is: Δ t2＝Δt1+ w + Δ, where Δ is a preset duration, and Δ ranges from 0 to w.
The above steps for realizing the corresponding functions of each parameter and each unit module in the background subtraction detection system 200 for raman spectroscopy according to the present invention can refer to each parameter and step in the above embodiments of the background subtraction detection method for raman spectroscopy, which are not described herein again. The method can also be improved and upgraded in the existing Raman spectrometer, and a program corresponding to the Raman spectrum-based quantitative detection method is embedded.
The raman spectrometer of the embodiment of the present invention includes a control chip, and the control chip is used in any one of the above background subtraction detection methods for raman spectroscopy.
As will be appreciated by one skilled in the art, the present invention may be embodied as a system, method or computer program product.
Accordingly, the present disclosure may be embodied in the form of: may be embodied entirely in hardware, entirely in software (including firmware, resident software, micro-code, etc.) or in a combination of hardware and software, and may be referred to herein generally as a "circuit," module "or" system. Furthermore, in some embodiments, the invention may also be embodied in the form of a computer program product in one or more computer-readable media having computer-readable program code embodied in the medium.
Any combination of one or more computer-readable media may be employed. The computer readable medium may be a computer readable signal medium or a computer readable storage medium. A computer readable storage medium may be, for example, but not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any combination of the foregoing. More specific examples (a non-exhaustive list) of the computer-readable storage medium include an electrical connection having one or more wires, a portable computer diskette, a hard disk, a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disc read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing. In the context of this document, a computer readable storage medium may be any tangible medium that can contain, or store a program for use by or in connection with an instruction execution system, apparatus, or device.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (7)
1. A method of background subtraction detection of raman spectra, comprising:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser emission according to the time sequence1To Δ t1Generating a Raman spectrum of the sample to be detected by Raman scattering light in + w time period, and simultaneously collecting a second time delay delta t after each pulse laser emission2To Δ t2Detecting background light of a background in a + w period, and generating a background spectrum of the detected background, wherein w is the pulse width of pulse laser emitted by a laser in the Raman spectrometer;
and deducting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected.
2. A method of background subtraction detection of raman spectra according to claim 1, wherein said first time-delayed acquisition process comprises:
calculating the first time delay at according to a first formula1The first formula is:
wherein L is the sampling probe of the Raman spectrometer and the sample to be measuredThe distance between samples, c is the speed of light.
3. The method of claim 2, wherein the step of obtaining the second time delay comprises:
calculating the second time delay at according to a second formula2The second formula is: Δ t2＝Δt1+ w + Δ, where Δ is a preset duration, and Δ ranges from 0 to w.
4. A background subtraction detection system of Raman spectrum is characterized by comprising an acquisition generation module and a subtraction module;
the acquisition generation module is used for:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser emission according to the time sequence1To Δ t1Generating a Raman spectrum of the sample to be detected by Raman scattering light in + w time period, and simultaneously collecting a second time delay delta t after each pulse laser emission2To Δ t2Detecting background light of a background in a + w period, and generating a background spectrum of the detected background, wherein w is the pulse width of pulse laser emitted by a laser in the Raman spectrometer;
the deduction module is configured to: and deducting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected.
5. The system of claim 4, further comprising an acquisition module configured to:
calculating the first time delay at according to a first formula1The first formula is:
wherein, L is the distance between a sampling probe of the Raman spectrometer and the sample to be measured, and c is the light speed.
6. The system of claim 5, wherein the acquisition module is further configured to:
calculating the second time delay at according to a second formula2The second formula is: Δ t2＝Δt1+ w + Δ, where Δ is a preset duration, and Δ ranges from 0 to w.
7. A raman spectrometer comprising a control chip for performing a method of background subtraction detection of a raman spectrum according to any one of claims 1 to 3.
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| CN105866099A (en) * | 2016-05-16 | 2016-08-17 | 天津大学 | Raman spectrum acquisition system with low-fluorescence background |
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| CN113092437A (en) * | 2021-03-25 | 2021-07-09 | 东莞市中科原子精密制造科技有限公司 | Remote Raman spectrum detection module and remote detection spectrometer |
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| CN105866099A (en) * | 2016-05-16 | 2016-08-17 | 天津大学 | Raman spectrum acquisition system with low-fluorescence background |
| CN106596499A (en) * | 2016-11-01 | 2017-04-26 | 北京华泰诺安技术有限公司 | Real-time Raman spectrum calibration method |
| GB2572662A (en) * | 2018-10-05 | 2019-10-09 | Res & Innovation Uk | Raman spectrometer |
| CN209707379U (en) * | 2019-02-21 | 2019-11-29 | 中智科仪（北京）科技有限公司 | Portable remote Raman spectrum system based on Gao Zhongying nanosecoud pulse laser |
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