CN113980816A - Alternaria brassicae and application thereof - Google Patents
Alternaria brassicae and application thereof Download PDFInfo
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- CN113980816A CN113980816A CN202110957184.0A CN202110957184A CN113980816A CN 113980816 A CN113980816 A CN 113980816A CN 202110957184 A CN202110957184 A CN 202110957184A CN 113980816 A CN113980816 A CN 113980816A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention relates to alternaria brassicae and application thereof, belonging to the technical field of microorganisms. The Alternaria brassicae (Alternaria brassicae) is preserved in China general microbiological culture Collection center (CGMCC) at 7-15 th of 2021, and the preservation number is CGMCC No. 23045. The liquid culture of the alternaria brassicae strain has obvious inhibition effect on 3 pathogenic fungi of wheat scab, corn northern leaf blight and orange brown spot pathogenic bacteria, and has wide application prospect.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to alternaria brassicae and application thereof in bacteriostasis.
Background
Alternaria brassicae (Alternaria brassicae) belongs to the kingdom of fungi, Ascomycota, Ascomycotina, Ascomycetes, Geospora and Sporomycoceae and is a common one in Alternaria. Alternaria spp is an important fungus in the fungi Deuterophaera, and the fungus is of various types and has wide host range and geographical distribution. More than 95% of species can parasitize plants in a facultative manner, and are important phytopathogens. Some are opportunistic pathogens of humans and animals, causing a variety of diseases. Some of them can be used as bactericide and herbicide, etc., and are biological resources with application potential.
Wheat scab is caused by Fusarium graminearum (a fungus), which not only causes great reduction in yield of wheat, but also can generate mycotoxin, and causes great loss to agricultural production and ecology. Due to factors such as increased rainfall, straw returning and the like, wheat scab frequently occurs in large areas in major production areas of wheat in China, becomes a main disease of wheat in Huang-Huai-Mai areas, and seriously affects the yield and quality of wheat.
Northern leaf blight is a serious leaf disease of maize, caused by the fungus northern leaf blight. The aponeurosis zeae is characterized in that the aponeurosis zeae in a non-sexual state (Exserohilum turcicum) is Helminthosporum zeae, and belongs to the asexual fungus Helminthosporum; the sexual state (Setosphaeria turcica) is setaria gigantea, belonging to the genus Trichosporon of the phylum Ascomycota. The northern leaf blight of corn is characterized by yellowing and early withering of plants, stalk turning, often withering in the ground and severe loss of corn yield. The occurrence and prevalence of northern leaf blight not only causes serious economic loss but also reduces the quality of corn. Since the 70 s, the disease is mainly distributed in cold and cool corn planting areas such as northern areas, high-altitude mountain areas and the like in China. The general annual yield reduction is about 20%, and in the seriously popular years and regions, the corn yield reduction is 50% or more.
The citrus brown spot is a defoliating disease caused by the fungus Alternaria alternate, and germs mainly damage tender leaves, new shoots and young fruits (the fruits can be damaged in the whole growth period of high-susceptibility varieties), cause defoliation, fruit drop and dead shoots, and cause the non-shedding fruits to cause the lesion spots to be not sold on the market. The fruit of the affected plant can then become infected and cause severe fruit drop just before fruit set and until harvest. The exocarpium citri rubrum is originally produced in China, mainly in Sichuan and Chongqing places, wherein Yunyang counties in the abdominal land of the three gorges reservoir area mainly plant the clovershrub tangerine, and the planting history is 4000 years. In recent years, the area of damage caused by citrus brown spot is expanding, and the quality of citrus red is seriously affected.
Plant diseases always seriously affect the quality and the yield of planted products, and the total yield of crops and medicinal plants at home and abroad is reduced by more than 10 percent due to the plant diseases every year, so the problems of poor quality and reduced total yield of the crops and the medicinal plants are solved, and the research on the prevention and the treatment of the plant diseases is urgent. The biological control has the advantages of good biological control effect, lasting pesticide effect period, no residue and the like, can avoid the defects caused by chemical control, and increasingly becomes the hot research direction of agricultural sustainable development.
Disclosure of Invention
The invention aims to solve the defects of the prior art and provides Alternaria brassicae and application thereof in bacteriostasis, the strain has the characteristics of easy culture, high yield, stable culture character and the like, and the culture has higher bacteriostatic activity on the 5 crop pathogenic fungi.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the invention provides Alternaria brassicae (Alternaria brassicensis) XL-0010 which is preserved in China general microbiological culture Collection center (CGMCC) at 7-15 th of 2021 with the preservation number of CGMCC No. 23045.
In a second aspect of the invention, the Alternaria brassicae XL-0010 is applied to preparation of a bacteriostatic agent.
Further, preferably, the fungus inhibited by the bacteriostatic agent is at least one of wheat scab pathogen, corn northern leaf blight pathogen and orange brown spot pathogen.
Further, it is preferable that the active ingredient in the bacteriostatic agent comprises the culture of Alternaria brassicae (Alternaria brassicensis) XL-0010.
Further, preferably, the active ingredient in the bacteriostatic agent comprises an extract of a culture of Alternaria brassicae (Alternaria brassicensis) XL-0010; the extract is obtained by extracting a culture of Alternaria brassicae (Alternaria brassicensis) XL-0010 serving as a raw material with an organic solvent.
In a third aspect, the invention provides a culture of Alternaria brassicae (Alternaria brassicolor) XL-0010, wherein the culture of Alternaria brassicae (Alternaria brassicolor) XL-0010 is a liquid culture; the culture method comprises the following steps: inoculating Alternaria brassicae (Alternaria brassicae) XL-0010 on a PDA slant culture medium by streaking, and culturing in an incubator at 28 ℃ for 3-5 days;
preparing a PDB liquid culture medium, directly inoculating the grown inclined plane into the PDB liquid culture medium, culturing for 15-20 days in a shaking table at 28 ℃ and 180rpm, and taking out to obtain a liquid culture;
the PDA slant culture medium comprises the following components in parts by weight: 3g/L of potato extract powder, 20g/L of glucose, 14g/L of agar and 5.6 +/-0.2 of pH value;
the PDB liquid culture medium formula comprises: 5g/L of potato extract powder, 15g/L of glucose, 10g/L of peptone and 5g/L of sodium chloride, and the pH value is natural.
In the fourth aspect, the invention provides the extract of the Alternaria brassicae XL-0010 culture, which is obtained by extracting the Alternaria brassicae XL-0010 culture with an organic solvent.
Further, preferably, the organic solvent is ethyl acetate.
The Alternaria brassicae XL-0010 culture can be obtained by culturing a PDA culture medium, a PDB culture medium and the like which are conventional in the field by taking Alternaria brassicae XL-0010 as a raw material.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a Alternaria brassicae and application thereof in bacteriostasis, the liquid culture of the Alternaria brassicae XL-0010 has obvious inhibition effect on 3 fungi of wheat scab (Fusarium graminearum, NB-05), northern leaf blight (NB-06) and orange brown spot pathogenic bacteria (Alternaria alternata), the bacteriostasis rate can reach 40% at most, and the application prospect is wide.
Drawings
FIG. 1 is a single colony morphology (PDA plate) of Alternaria brassicae XL-0010; wherein, FIG. 1-A is a front view of the plate; FIG. 1-B is a back view of the plate.
FIG. 2 shows the inhibition of 3 plant pathogenic fungi by Alternaria brassicae XL-0010 (PDA plate); wherein, FIG. 2-A is a front view of the plate; FIG. 2-B is a back view of a flat panel
Alternaria brassicae (Alternaria brassicae) XL-0010 is preserved in China general microbiological culture Collection center (CGMCC No. 23045) at 7-15 days 2021, with the preservation number of CGMCC No.23045, and the preservation address of No. 3 Hospital No.1, institute of microbiology, China academy of sciences, in the area facing the sun, Beijing.
Detailed Description
The present invention will be described in further detail with reference to examples.
It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The materials or equipment used are not indicated by manufacturers, and all are conventional products available by purchase.
Example 1 sources and identification of Alternaria brassicae (Alternaria brassica) XL-0010
Sources of Alternaria brassicae XL-0010
A fungus is separated from cassia tora picked in Shenyang pharmaceutical university botanical garden in Liaoning province in 2017, 9 and 4 days, and is named as XL-0010.
② identification of strain XL-0010
The ITS sequencing Sequence of XL-0010 is shown in a Sequence table (SEQ ID NO.1), and the Sequence similarity of the ITS sequencing Sequence with Sequence ID: MW008951.1 is the highest, and the similarity is 99.62%.
Based on the above identification results, XL-0010 belongs to the species Alternaria brassicae (Alternaria brassicca).
Example 2 Main morphological characteristics of Alternaria brassicae (Alternaria brassicensis) XL-0010
The alternaria brassicae XL-0010 is inoculated on a potato glucose culture medium and cultured at a constant temperature of 28 ℃, the colony is white at the initial stage and is radially expanded, the mycelium is dense, the colony is round, the edge is neat, the aerial mycelium is developed, and the color of the colony gradually becomes dark after 3 days and slowly becomes grey brown. Conidiophores are dark, single branch, different in length and terminal conidiophores. Conidia are dark, the conidia have 6-12 transverse diaphragms, 0-6 longitudinal and oblique diaphragms, 64.0-158.0 multiplied by 19.5-38.0 μm of conidia bodies, and 23.0-93.0 multiplied by 6.0-8.0 μm of beaks.
The PDA culture medium formula comprises: 3g/L of potato extract powder, 20g/L of glucose, 14g/L of agar and 5.6 +/-0.2 of pH value.
The single colony morphology of the strain on a PDA plate is shown in figure 1, and figure 1-A is a front view of the plate in figure 1-A; FIG. 1-B is a back view of the plate.
Example 3 determination of the rate of inhibition of pathogenic fungi by Alternaria brassicae (Alternaria brassicae) XL-0010
The plate confronting method is adopted to determine the bacteriostatic ability of the fungus to be screened (alternaria brassicae XL-0010) to the tested fungus (tens of pathogenic fungi), and the specific method is as follows:
preparing PDA plate, culturing the fungi to be screened and the fungi to be tested respectively, after the colony diameter reaches 70-80mm, punching holes (equidistant from the edge of the dish) on the positions with consistent growth of the two culture mediums respectively by using a sterilized 5mm puncher. On the empty PDA plate, the perforated culture medium blocks were picked up with sterilized tweezers, and two kinds of fungi were inoculated in reverse directions at about 3cm on the same line from the center of the plate, and then the zone of inhibition was measured every 24 hours (cross method). The bacteriostatic rate was calculated according to the following formula:
the inhibition rate was [ (control colony diameter-5) - (treated colony diameter-5) ]/(control colony diameter-5) × 100%
Note: "5" is the diameter of the colony taken by the punch.
The PDA culture medium formula comprises: 3g/L of potato extract powder, 20g/L of glucose, 14g/L of agar and 5.6 +/-0.2 of pH value.
The inhibition rate of the alternaria brassicae XL-0010 on different pathogenic fungi is shown in table 1 (only the test fungi with the inhibition rate of the fungi to be screened higher than 20 percent on the test fungi are recorded in the table).
TABLE 1 inhibition of Alternaria brassicae XL-0010 against different pathogenic fungi
| Selecting test fungi | The antibacterial rate is% |
| NB-05 | 31.84％ |
| NB-06 | 46.00％ |
| YB-05 | 29.06％ |
Wherein NB-05 is Fusarium graminearum, NB-06 is northern leaf blight (Setosphaeria turcica), YB-05 is orange brown spot pathogen (Alternaria alternata).
As can be seen from Table 1, XL-0010 has strong bacteriostatic activity on 3 plant pathogenic fungi, wherein the bacteriostatic rate of the bacterial strain northern leaf blight (NB-06) is 46.00%.
The inhibition effect of the strain on 3 plant pathogenic fungi on a PDA plate is shown in figure 2, the upper half part of the plate is 3 plant pathogenic fungi, the lower half part of the plate is alternaria brassicae XL-0010, wherein figure 2-A is a front view of the plate; FIG. 2-B is a back view of the plate. As can be seen from figure 2, the Alternaria brassicae XL-0010 has obvious inhibition effect on 3 plant pathogenic fungi.
Example 4 liquid culture of Alternaria brassicae (Alternaria brassicae) XL-0010
Activation of the strain: inoculating the preserved Alternaria brassicae XL-0010 on a PDA slant culture medium by streaking, and culturing in an incubator at 28 ℃ for 3-5 days;
liquid culture of the strain: preparing 200 ml/bottle PDB liquid culture medium, directly inoculating the grown slant into a liquid shake flask, culturing in a shaking table at 28 deg.C and 180rpm for 15-20 days, and taking out to obtain liquid culture (i.e. fermentation liquid).
The PDA slant culture medium comprises the following components in parts by weight: 3g/L of potato extract powder, 20g/L of glucose, 14g/L of agar and 5.6 +/-0.2 of pH value.
The PDB liquid culture medium formula comprises: 5g/L of potato extract powder, 15g/L of glucose, 10g/L of peptone and 5g/L of sodium chloride, and the pH value is natural.
Example 5 preparation of crude Ethyl acetate extract of Alternaria brassicae (Alternaria brassicae) XL-0010
The fermentation liquor of example 4 was extracted with an equal volume of ethyl acetate by the following method: oscillating for 30min to fully mix the fermentation liquor with ethyl acetate, standing for 24h for liquid separation, taking out the ethyl acetate phase, namely the upper phase, performing reduced pressure evaporation at 42 ℃ (the vacuum degree is generally-0.08 MPa to-0.01 MPa), completely evaporating the solvent by using a rotary evaporator to obtain a crude extract of the bacterial liquid ethyl acetate, and preserving in a refrigerator at 4 ℃ for later use.
Example 6 determination of MIC and MBC values of crude Ethyl acetate extract of Alternaria brassicae (Alternaria brassicae) XL-0010
The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the crude extract of the bacterial liquid ethyl acetate in example 5 are further determined by a 96-well plate method, and the specific method is as follows:
culture of test bacterial liquid: and (3) streaking and inoculating the preserved test fungi (3 pathogenic fungi) on a PDA slant culture medium, culturing in an incubator at 28 ℃ for 3-5 days, directly inoculating the grown slant into a standby PDB liquid culture medium, culturing in a shaking table at 28 ℃ and 180rpm for 48h, and taking out for standby to obtain the test bacterial liquid.
Preparing a sample solution: 1mg of the crude ethyl acetate extract of the bacterial liquid obtained in the embodiment 5 is taken, 100uLDMSO is added to be fully dissolved, and then the volume is determined by DMSO to obtain a sample solution of 100 ug/mL.
Measuring MIC and MBC by a 96-well plate method: taking a sterile 96-well plate, respectively adding 100 mu L of PDB liquid culture medium into 1-7 wells, taking 100 mu L of sample solution to add into No.1 well, uniformly mixing (the concentration is 50ug/mL), taking 100 mu L of sample solution out of No.1, adding into No.2 well, uniformly mixing, sequentially diluting to No. 7 well, taking 100 mu L of sample solution out of No. 7 well, and discarding. The sample concentration in the No. 1-7 holes is 50, 25, 12.5, 6.25, 3.125, 1.563 and 0.7815ug/mL in sequence, and then 100 mul of test bacteria liquid is added respectively. On the same 96-well plate, a hole added with 100 mu LDMSO is used as a blank control, a hole added with only 100 mu L of test bacteria liquid without adding sample solution is used as a positive control, and a hole added with only 100 mu L of PDB liquid culture medium without adding test bacteria liquid is used as a negative control. The minimum clear concentration of the culture broth in the wells was observed as MIC, 50. mu.L of the clarified broth from the wells was applied to PDA medium, and inverted culture was carried out at 28 ℃ for 48 hours, which was repeated three times, and the minimum concentration of MBC at which no bacteria grew on PDA medium at all was observed.
The PDA culture medium formula comprises: 3g/L of potato extract powder, 20g/L of glucose, 14g/L of agar and 5.6 +/-0.2 of pH value.
The PDB liquid culture medium formula comprises: 5g/L of potato extract powder, 15g/L of glucose, 10g/L of peptone and 5g/L of sodium chloride, and the pH value is natural.
The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the alternaria brassicae XL-0010 to different pathogenic fungi are shown in Table 2.
TABLE 2 Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) of Alternaria brassicae XL-0010 against various pathogenic fungi
| Selecting test fungi | MIC/(ug/mL) | MBC/(ug/mL) |
| NB-05 | 6.25 | 50 |
| NB-06 | 0.7815 | 12.5 |
| YB-05 | 12.5 | 50 |
As can be seen from Table 2, the ethyl acetate crude extract of the XL-0010 liquid culture has significant inhibitory effects on 3 pathogenic fungi, namely wheat scab pathogen, corn northern leaf blight pathogen and orange brown spot pathogen.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Sequence listing
<110> Yangling future Zhongke environmental protection science and technology Limited
<120> Alternaria brassicae and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 543
<212> DNA
<213> 1 (Artificial sequence)
<400> 1
cgctccatgg acgcgggctg gacctctcgg ggttacagcc ttgctgaatt attcaccctt 60
gtcttttgcg tacttcttgt ttccttggtg ggttcgccca ccactaggac aaacataaac 120
cttttgtaat tgcaatcagc gtcagtaaca aattaataat tacaactttc aacaacggat 180
ctcttggttc tggcatcgat gaagaacgca gcgaaatgcg ataagtagtg tgaattgcag 240
aattcagtga atcatcgaat ctttgaacgc acattgcgcc ctttggtatt ccaaagggca 300
tgcctgttcg agcgtcattt gtaccctcaa gctttgcttg gtgttgggcg tcttgtctct 360
agctttgctg gagactcgcc ttaaagtaat tggcagccgg cctactggtt tcggagcgca 420
gcacaagtcg cactctctat cagcaaaggt ctagcatcca ttaagccttt ttttcaactt 480
ttgacctcgg atcaggtagg gatacccgct gaacttaagc atatcaaaag acgggaagaa 540
aat 543
Claims (8)
1. Alternaria brassicae (Alternaria brassicae) XL-0010 is preserved in China general microbiological culture Collection center (CGMCC) at 7-15 days 2021, and the preservation number is CGMCC No. 23045.
2. Use of Alternaria brassicae (Alternaria brassicae) XL-0010 as defined in claim 1 for the preparation of a bacteriostatic agent.
3. The use of Alternaria brassicae (Alternaria brassicca) XL-0010 in the preparation of a bacteriostatic agent according to claim 2, wherein the fungus inhibited by the bacteriostatic agent is at least one of fusarium graminearum, maculopathy zeae and cercospora citricola.
4. The use of Alternaria brassicae (Alternaria brassicca) XL-0010 in the preparation of a bacteriostatic agent according to claim 2, wherein the active ingredient in the bacteriostatic agent comprises the culture of Alternaria brassicae (Alternaria brassicca) XL-0010.
5. Use of Alternaria brassicae (Alternaria brassicae) XL-0010 according to claim 4 for the preparation of a bacteriostatic agent, wherein the active ingredient in said bacteriostatic agent comprises an extract of a culture of Alternaria brassicae (Alternaria brassicae) XL-0010; the extract is obtained by extracting a culture of Alternaria brassicae (Alternaria brassicensis) XL-0010 serving as a raw material with an organic solvent.
6. The culture of Alternaria brassicae (Alternaria brassicca) XL-0010 according to claim 4, wherein said culture of Alternaria brassicae (Alternaria brassicca) XL-0010 is a liquid culture; the culture method comprises the following steps: inoculating Alternaria brassicae (Alternaria brassicae) XL-0010 on a PDA slant culture medium by streaking, and culturing in an incubator at 28 ℃ for 3-5 days;
preparing a PDB liquid culture medium, directly inoculating the grown inclined plane into the PDB liquid culture medium, culturing for 15-20 days in a shaking table at 28 ℃ and 180rpm, and taking out to obtain a liquid culture;
the PDA slant culture medium contains: 3g/L of potato extract powder, 20g/L of glucose, 14g/L of agar and 5.6 +/-0.2 of pH value;
the PDB liquid culture medium contains: 5g/L of potato extract powder, 15g/L of glucose, 10g/L of peptone and 5g/L of sodium chloride.
7. The extract of the culture of Alternaria brassicae (Alternaria brassicolor) XL-0010 according to claim 5, which is obtained by extracting the culture of Alternaria brassicae (Alternaria brassicolor) XL-0010 according to claim 6 with an organic solvent.
8. The extract of culture of Alternaria brassicae (Alternaria brassicae) XL-0010 according to claim 7, characterized in that said organic solvent is ethyl acetate.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0975070A (en) * | 1995-09-18 | 1997-03-25 | Japan Tobacco Inc | Microorganism belonging to genus alternaria and its use |
| US20100291039A1 (en) * | 2007-12-14 | 2010-11-18 | Kohl Jurgen Anton | Novel micro-organisms controlling plant pathogens |
| CN102191184A (en) * | 2011-04-08 | 2011-09-21 | 中国计量学院 | Biocontrol endophytic fungi-Alternaria alternata |
| CN109897788A (en) * | 2019-03-08 | 2019-06-18 | 浙江工业大学 | A kind of rape rod method and preparing the application in bacteriostatic agent |
-
2021
- 2021-08-19 CN CN202110957184.0A patent/CN113980816B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0975070A (en) * | 1995-09-18 | 1997-03-25 | Japan Tobacco Inc | Microorganism belonging to genus alternaria and its use |
| US20100291039A1 (en) * | 2007-12-14 | 2010-11-18 | Kohl Jurgen Anton | Novel micro-organisms controlling plant pathogens |
| CN102191184A (en) * | 2011-04-08 | 2011-09-21 | 中国计量学院 | Biocontrol endophytic fungi-Alternaria alternata |
| CN109897788A (en) * | 2019-03-08 | 2019-06-18 | 浙江工业大学 | A kind of rape rod method and preparing the application in bacteriostatic agent |
Non-Patent Citations (1)
| Title |
|---|
| 苗智等: "1 株链格孢属真菌生物碱类代谢产物的研究", 《江苏农业科学》 * |
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