CN113975217A - Whitening and anti-aging composition based on herbaceous plants and preparation method and application thereof - Google Patents
Whitening and anti-aging composition based on herbaceous plants and preparation method and application thereof Download PDFInfo
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- CN113975217A CN113975217A CN202111459582.6A CN202111459582A CN113975217A CN 113975217 A CN113975217 A CN 113975217A CN 202111459582 A CN202111459582 A CN 202111459582A CN 113975217 A CN113975217 A CN 113975217A
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Classifications
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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Abstract
The invention provides a whitening anti-aging composition based on herbaceous plants, which comprises a lily extract, a peach kernel extract, a kelp extract and acerola powder; the invention also provides a preparation method and application of the whitening anti-aging composition based on the herbaceous plants; according to the whitening and anti-aging composition based on the herbaceous plants, the skin pigments (melanin and yellow pigment) can be regulated and controlled through the lily extract and the peach kernel extract, and the synthesis and the transportation of the pigments are inhibited; the semen Persicae extract and thallus laminariae extract can promote blood microcirculation and increase haematochrome content; thallus laminariae extract and acerola powder can reduce oxidative stress and inflammation of skin tissue; and appropriate amount of collagen peptide and elastin are taken, so that collagen required by the skin can be supplemented, the purposes of whitening, resisting aging, moisturizing, resisting allergy and protecting the skin are achieved, the problems of skin dryness, skin allergy and the like caused by whitening can be avoided, and the product has a good market prospect.
Description
Technical Field
The invention belongs to the field of food cosmetics, and particularly relates to a whitening and anti-aging composition, a preparation method and an application thereof, in particular to a whitening and anti-aging composition based on herbaceous plants, and a preparation method and an application thereof.
Background
The skin which is white, tender, fine and glossy is the ever-present pursuit of people, and as time goes away, the skin shows more or less spots, dark yellow, roughness and wrinkles, which causes various reasons, such as slow metabolism, poor blood circulation, long-term staying up all night, melanin accumulation, loss of collagen in the skin, reduction of hyaluronic acid content, weakening of water retention function of the skin and the like.
At present, the whitening products in the market mainly contain chemical synthetic substances with whitening and antioxidant effects, such as arbutin, hydroquinone, kojic acid, BHT, vitamin C and derivatives thereof. Although the chemical synthesis effect compounds have remarkable effect, the problems of large irritation, instability, single effect and the like are gradually exposed in the using process. However, since a lot of herbs in nature contain huge energy and have the effects of whitening, resisting wrinkles, fading color spots and smoothing skin, the development of a skin beautifying formula which has high safety level, multiple effects and good stability and is derived from natural active ingredients of plants becomes a focus of attention of research and development institutions and manufacturing enterprises in recent years.
CN106260363A discloses a herbal tea for whitening and beautifying, which is prepared from raw materials of jujube, kelp, peach kernel, Chinese yam, medlar, lily, purslane, honeysuckle and crystal sugar, and the herbal tea has obvious effects on improving common problems of dark yellow, rough and lack of elasticity of skin, but only focuses on whitening effect, and mainly functions on inhibiting synthesis of skin melanin and promoting melanin metabolism of skin.
CN105614719A discloses a donkey-hide gelatin cake with effects of whitening skin, removing freckles, which is prepared from 28 components such as cherry, walnut, red date, honey, rose, peanut and cymbidium floribundum, and has the pharmaceutical efficacy, can effectively whiten skin, remove freckles, improve immunity of organism and improve sleep quality, but the donkey-hide gelatin is used as a main component, and is matched with other numerous plant components, so that the formula is complex and the preparation process is complex.
The variety of the herbaceous plants is wide, and the result of compounding different herbaceous plant extracts has great uncertainty, so that the plant extracts are combined into a formula which has the whitening and anti-aging effects, is reasonable, efficient and safe, and has important market value.
Disclosure of Invention
The invention aims to provide a whitening and anti-aging composition and a preparation method and application thereof, and particularly provides a whitening and anti-aging composition based on herbaceous plants and a preparation method and application thereof. Compared with other similar whitening oral products, the whitening anti-aging composition based on the herbaceous plants provided by the invention does not start from a single action mechanism of inhibiting melanin to enable the product to achieve a whitening effect, and adopts components for regulating and controlling melanin, yellow pigment and red pigment active components, adding water replenishing, moisturizing, anti-allergy and enhancing skin immune barrier, and combining a multi-dimensional whitening mechanism to achieve the full-aspect whitening effect, so that the skin aging phenomena such as wrinkles, color spots, dark yellow and the like can be relieved under the condition of not stimulating the skin, and the side effects such as water consumption, skin dryness, barrier damage and the like caused by accelerated skin metabolism in the whitening regulation process can be relieved.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a herbal-based whitening anti-aging composition, the components of which comprise lily extract, peach kernel extract, kelp extract and acerola powder.
In the whitening and anti-aging composition, the lily extract and the peach kernel extract can regulate and control skin pigments (melanin and yellow pigment) and inhibit pigment synthesis and transportation; the semen Persicae extract and thallus laminariae extract can promote blood microcirculation and increase haematochrome content; the kelp extract and the acerola cherry powder can reduce the oxidative stress and the inflammatory state of skin tissues, moisten the skin and relieve wrinkles.
Preferably, the whitening and anti-aging composition based on the herbaceous plants comprises 15.5-35.5 parts of lily extract, 15-35 parts of peach kernel extract, 10.5-20.5 parts of kelp extract and 9.5-20 parts of acerola powder in parts by weight.
When the components in the whitening and anti-aging composition are mixed according to the specific parts by weight, the whitening and anti-aging effect on skin is more remarkable, and the side effects of water consumption, skin dryness, barrier damage and the like caused by accelerated skin metabolism in the whitening regulation and control process can be relieved.
Preferably, the parts by weight of the lily extract can be 16 parts, 18 parts, 20 parts, 22 parts, 25 parts, 27 parts, 30 parts, 32 parts, 34 parts, 35 parts and the like, and other specific point values in the numerical range can be selected, so that the description is omitted.
Preferably, the parts by weight of the peach kernel extract can be 16 parts, 18 parts, 20 parts, 22 parts, 25 parts, 27 parts, 30 parts, 32 parts, 34 parts and the like, and other specific point values in the numerical range can be selected, so that the description is omitted.
Preferably, the kelp extract can be 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts and the like by weight, and other specific point values in the numerical value range can be selected, so that the details are not repeated.
Preferably, the parts by weight of the acerola cherry powder can be 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts and the like, and other specific point values in the numerical value range can be selected, so that the description is omitted.
Preferably, the components of the herbal-based whitening anti-aging composition further include collagen peptides and/or elastin peptides.
In the invention, by taking a proper amount of collagen peptide and/or elastin peptide, the collagen required by the skin can be supplemented, the fibrous tissue of the skin can be reformed, the cell metabolism can be promoted, the moisture in the skin can be locked strongly, and the product has better effects of whitening, resisting aging and improving the skin quality.
Preferably, the collagen peptide is 3 to 15 parts by weight, for example, 4 parts, 6 parts, 8 parts, 10 parts, 12 parts, 14 parts, and the like, and other specific values within the numerical range can be selected, which is not described herein again.
Preferably, the elastin peptide is 2.5-9.5 parts by weight, such as 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts and the like, and other specific values in the numerical range can be selected, and are not repeated herein.
Preferably, the herbal plant-based whitening anti-aging composition comprises 25-30 parts by weight of lily extract, 25-30 parts by weight of peach kernel extract, 15-18 parts by weight of kelp extract, 12-15 parts by weight of acerola powder, 3-15 parts by weight of collagen peptide and 2.5-9.5 parts by weight of elastin peptide.
In a second aspect, the present application provides a method for preparing the herbal-based whitening anti-aging composition according to the first aspect, the method comprising: mixing and stirring uniformly lily extract, peach kernel extract, kelp extract and acerola powder according to the formula amount to obtain the whitening anti-aging composition.
Preferably, the method comprises the steps of:
(1) firstly, mixing and stirring lily extract, peach kernel extract, kelp extract and acerola cherry powder uniformly according to the formula amount to obtain a mixture A;
(2) premixing collagen peptide and elastin peptide with part of the mixture A obtained in the step (1) to obtain a premix B;
(3) and (3) uniformly mixing and stirring the premix B obtained in the step (2) and the rest mixture A obtained in the step (1) to obtain the whitening and anti-aging composition.
Preferably, the stirring temperature in step (1) is 20-25 ℃ (for example, 21 ℃, 22 ℃, 23 ℃, 24 ℃ and the like can be achieved), and the stirring time is 10-20min (for example, 12min, 14min, 15min, 16min, 18min and the like can be achieved).
Preferably, the humidity during the mixing and stirring in the step (1) is controlled to be 35-45% (for example, 37%, 39%, 40%, 41%, 43%, etc.).
Preferably, the temperature during the pre-mixing in step (2) is 20-30 ℃ (for example, 22 ℃, 24 ℃, 25 ℃, 26 ℃, 28 ℃, etc. can be adopted).
Preferably, the part of the mixture A obtained in step (1) in step (2) is 50% to 70% (for example, 53%, 55%, 58%, 60%, 63%, 65%, 68% and the like) of the total amount of the mixture A obtained in step (1).
Preferably, the stirring temperature in step (3) is 25-30 deg.C (e.g. 26 deg.C, 27 deg.C, 28 deg.C, 29 deg.C, etc.), and the stirring time is 15-25min (e.g. 17min, 19min, 20min, 22min, 24min, etc.).
Preferably, the humidity during the mixing and stirring in the step (3) is controlled to be 45-55% (for example, 47%, 49%, 50%, 52%, 54%, etc.).
The specific point values in the above ranges can be selected, and are not described in detail herein.
In the invention, the preparation method ensures that the main components such as lily extract, peach kernel extract, kelp extract, acerola powder and the like are not damaged and can be fully and uniformly mixed by controlling the temperature, the humidity and the mixing time; if the temperature is too high, the main components of the herbaceous plants can be damaged, and if the temperature is too low, the main components cannot be fully mixed; when the humidity is too high, the moisture absorption of the herbaceous plants influences the water content and the properties of the mixture; if the humidity is too low, the mixing uniformity is affected; the time is too short, the mixing effect can not be achieved, and the time is too long, so that not only is energy wasted, but also excessive mixing of the powder is caused, and the components and the uniformity of the powder are damaged; within the above defined range, the best results are achieved.
In a third aspect, the present invention provides a use of the herbal-based whitening anti-aging composition according to the first aspect in preparing food, health products or cosmetics.
Preferably, the food product comprises a tabletted candy, a gel candy, a liquid beverage or a solid beverage.
In a fourth aspect, the invention provides a tablet candy, which comprises the following preparation raw materials in parts by weight:
30 to 60 parts (for example, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts and the like) of the herbal whitening anti-aging composition according to the first aspect, 10 to 17 parts (for example, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts and the like) of microcrystalline cellulose, 15 to 28 parts (for example, 17 parts, 20 parts, 22 parts, 25 parts, 27 parts and the like) of isomalt, 0.01 to 1 part (for example, 0.05 part, 0.1 part, 0.3 part, 0.5 part, 0.7 part, 0.9 part and the like) of sodium carboxymethylcellulose, 1 to 5 parts (for example, 1.5 parts, 2 parts, 3 parts, 4 parts, 4.5 parts and the like) of silicon dioxide, 1 to 5 parts (for example, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts and the like) of magnesium stearate, and the like.
Preferably, the method of preparing the tabletted confectionery comprises:
(1) one-step granulation: putting the whitening and anti-aging composition into a granulator, adding sodium carboxymethylcellulose as an adhesive, controlling the temperature and the moisture, and sieving to obtain a granule mixture during discharging;
(2) total mixing: mixing the mixed particles obtained in the step (1) with microcrystalline cellulose, silicon dioxide and isomaltitol in a three-dimensional mixer for the first time, and then adding magnesium stearate for mixing for the second time to obtain uniformly mixed total mixed powder;
(3) tabletting: and tabletting the total mixed powder by using a high-speed tabletting machine, and controlling the tablet weight to obtain the tabletted candy.
Preferably, the concentration of the sodium carboxymethyl cellulose in the step (1) is 0.5% -1.5%.
Preferably, the temperature in step (1) is 70-80 ℃.
Preferably, the moisture is controlled to 2-3% in the step (1).
Preferably, the mesh number of the screen in the step (1) is 16-20 meshes.
Preferably, the time for the first mixing in the step (2) is 15-25min, and the time for the second mixing is 5-10 min.
Preferably, the weight range in step (3) is 645 and 655 mg.
In a fifth aspect, the invention provides a gel candy, which comprises the following preparation raw materials in parts by weight: 2-15 parts (for example, 4 parts, 6 parts, 8 parts, 10 parts, 12 parts and 14 parts) of the herbal whitening anti-aging composition, 3-10 parts (for example, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts and 9 parts) of a gel, 40-60 parts (for example, 45 parts, 58 parts, 50 parts, 53 parts, 55 parts and 58 parts) of a sweetener, 1-4 parts (for example, 1.5 parts, 2 parts, 2.5 parts, 3 parts and 3.5 parts) of a collagen casing, 3-7 parts (for example, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts and 6.5 parts) of a flavoring essence (for example, 0.22 part, 0.24 part, 0.25 part, 0.26 part and 0.28 part) of purified water and 8-30 parts (for example, 10 parts, 12 parts, 15 parts, 20 parts, 22 parts and 10 parts) of purified water, 25 parts, 28 parts).
Preferably, the preparation method of the gel candy comprises the following steps:
(1) dissolving the whitening and anti-aging composition in water, and filtering to prepare a compound composition liquid;
dissolving a sweetening agent in water to obtain a sugar solution;
sol: mixing the gel with water, heating, and dissolving to obtain gel solution;
boiling: mixing the obtained glue solution and the sugar solution, uniformly stirring, and heating and boiling to obtain a mixed feed liquid;
(2) mixing: pouring the compound combined liquid, the edible essence and the concentrated fruit juice in the step (1) into a seasoning pot respectively, and mixing and stirring the compound combined liquid, the edible essence and the concentrated fruit juice with the mixed liquid in the step (1) uniformly to obtain soft candy syrup;
(3) performing clysis: filling the collagen casing into an automatic casing filling and twisting machine, transferring the soft candy syrup obtained in the step (2) into the automatic casing filling and twisting machine for filling, filling the soft candy syrup into the collagen casing by the automatic casing filling and twisting machine according to the weight requirement to form the casing soft candy with corresponding length, and obtaining the casing soft candy;
(4) and (3) cooling and forming: putting the sausage casing soft sweets in the step (3) into a cooling tunnel, and carrying out rapid cooling forming;
(5) polishing: and (4) shearing the cooled and formed sausage casing soft sweets in the step (4), shearing the sausage casing soft sweets at the ligation opening into single products, applying oil, polishing and packaging to obtain finished sausage casing soft sweets.
Preferably, the weight ratio of the water addition amount to the whitening and anti-aging composition in the compound combined liquid in the step (1) is (1-1.5): 1.
Preferably, the weight ratio of the water added to the sweetener in the sugar liquid in the step (1) is (0.5-1): 1.
Preferably, the weight ratio of the water adding amount to the gelling agent in the sol preparation in the step (1) is (1-1.5): 1.
Preferably, the temperature is raised to 138-.
Preferably, the temperature in the cooling tunnel in the step (4) is 0-10 ℃, and the relative humidity is 40-70%.
Preferably, the time for the rapid cooling forming in the step (4) is 5-10 min.
In a sixth aspect, the invention provides a liquid beverage, which comprises the following preparation raw materials in parts by weight: 10-30 parts (for example, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts and 28 parts) of the herbal whitening anti-aging composition, 10-20 parts (for example, 12 parts, 14 parts, 15 parts, 16 parts, 18 parts and 19 parts) of erythritol, 30-60 parts (for example, 35 parts, 40 parts, 45 parts, 50 parts and 55 parts) of purified water, 0.01-0.2 part (for example, 0.02 part, 0.05 part, 0.08 part, 0.1 part, 0.15 part and 0.18 part) of sweetening agent and 0.01-4 parts (for example, 0.5 part, 1 part, 1.5 part, 2 parts, 2.5 parts, 3 parts and 3.5 parts) of acid regulator.
Preferably, the method of preparing the liquid beverage comprises:
(1) preparation: putting the whitening and anti-aging composition based on the herbaceous plants into a preparation tank, adding water for the first time, mixing and stirring uniformly, heating, stirring for the first time, then adding other residual materials, adding water for the second time, stirring for the second time until the mixture is uniformly mixed, and then sieving the mixture into a storage tank;
(2) washing the bottle: cleaning the glass bottle by using an automatic cleaning machine;
(3) filling: subpackaging the liquid by a full-automatic filling machine;
(4) and (3) sterilization: sterilizing the bottled beverage by using a high-pressure steam sterilization pot, and cooling the bottled beverage after sterilization at a high temperature;
(5) and (4) lamp inspection: and (5) carrying out light inspection on the sterilized product after cooling and airing.
Preferably, the weight ratio of the first water adding amount to the whitening and anti-aging composition in the step (1) is (1-1.5): 1.
Preferably, the heating temperature in step (1) is 70-80 ℃.
Preferably, the first stirring time in the step (1) is 3-5 min.
Preferably, the second stirring time in the step (1) is 5-15 min.
Preferably, the mesh number of the screen in the step (1) is 150-250 meshes.
Preferably, the high temperature in step (4) is 110-120 ℃.
Preferably, the time for sterilization in step (4) is 15-25 min.
In a seventh aspect, the invention provides a solid beverage, which comprises the following preparation raw materials in parts by weight: 30-60 parts (for example, 35 parts, 40 parts, 45 parts, 50 parts and 55 parts) of the herbal whitening anti-aging composition, 0.01-1 part (for example, 0.1 part, 0.2 part, 0.5 part, 0.7 part, 0.8 part and 0.9 part) of sucralose, 0.01-4.5 parts (for example, 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts and 4 parts) of flavoring essence and 15-50 parts (for example, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts and 45 parts) of resistant dextrin.
Preferably, the preparation method of the solid beverage comprises the following steps:
(1) premixing: dispersing and mixing sucralose, edible essence and a proper amount of resistant dextrin to obtain a premix;
(2) total mixing: putting a small amount of resistant dextrin, the whitening anti-aging composition, the premix obtained in the step (1) and the residual resistant dextrin into a three-dimensional mixer in sequence for mixing, and discharging after mixing to obtain total mixed powder;
(3) subpackaging: and (3) subpackaging the total mixed powder in the step (2) by using a multi-row subpackaging machine, and controlling the net content of the product.
Preferably, the amount of resistant dextrin used in step (1) is one fifth of the amount of the formula.
Preferably, the small amount of resistant dextrin of step (2) is one third of the amount of the formula.
Preferably, the mixing time of step (2) is 15-25 min.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the lily extract, the peach kernel extract, the kelp extract and the acerola powder are creatively combined, four components supplement each other and synergize, so that the finally obtained whitening and anti-aging composition based on the herbaceous plants has remarkable whitening and anti-aging and healthy skin effects on human skin.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
The specification information of the part of the raw materials involved in the following examples and comparative examples is as follows:
| raw materials | Specification of |
| Lily extract | The polysaccharide is more than or equal to 10 percent |
| Peach kernel extract | The polysaccharide is more than or equal to 10 percent |
| Laminaria japonica extract | The laminarin content is more than or equal to 20 percent |
| Acerola cherry powder | Vitamin C is more than or equal to 15 percent |
The preparation method of the lily extract comprises the following steps:
lily extract: mashing Bulbus Lilii, placing into the processing chamber of PEF (high voltage pulsed electric field) extractor, and adding 10 times of water. Subjecting the sample to pulse treatment for 45 times at 3.0KV/cm to obtain water extractive solution, filtering, concentrating to obtain Bulbus Lilii extract, and spray drying the extract with appropriate amount of maltodextrin to obtain Bulbus Lilii extract;
peach kernel extract: the pulverized peach kernel is put into a processing chamber of a PEF (high-voltage pulsed electric field) extractor, and 10 times of water is added. Subjecting the sample to pulse treatment for 50 times at 4.0KV/cm to obtain water extractive solution, filtering, concentrating to obtain semen Persicae extract, and spray drying the extract with appropriate amount of maltodextrin to obtain semen Persicae extract;
kelp extract: placing the cut thallus laminariae into a processing chamber of PEF (high voltage pulsed electric field) extractor, and adding 10 times of water. Subjecting the sample to pulse treatment at 3.5KV/cm for 35 times to obtain water extractive solution, filtering, concentrating to obtain thallus laminariae extract, and spray drying the extract with appropriate amount of maltodextrin to obtain thallus laminariae extract.
The remaining raw materials may be any commercially available product that satisfies the above-mentioned specifications through a normal channel.
Example 1
The embodiment provides a whitening and anti-aging composition based on herbaceous plants, which comprises, by weight, 27 parts of lily extract, 28 parts of peach kernel extract, 16 parts of kelp extract, 13 parts of acerola powder, 10 parts of collagen peptide and 5 parts of elastin peptide.
The preparation method of the whitening anti-aging composition based on the herbaceous plants comprises the following steps:
(1) mixing Bulbus Lilii extract, semen Persicae extract, thallus laminariae extract, and acerola cherry powder at 23 deg.C and humidity of 38% for 15min to obtain mixture A;
(2) taking 3/5 amount of the mixture A obtained in the step (1), and then premixing with collagen peptide and elastin peptide, wherein the premixing temperature is 28 ℃ to obtain premix B;
(3) and (3) mixing and stirring the premix B obtained in the step (2) and the rest mixture A obtained in the step (1) at the temperature of 28 ℃ and the humidity of 50% for 20min to obtain the whitening and anti-aging composition.
Example 2
The embodiment provides a whitening and anti-aging composition based on herbaceous plants, which comprises the following components, by weight, 15.5 parts of a lily extract, 15 parts of a peach kernel extract, 10.5 parts of a kelp extract, 9.5 parts of acerola powder, 3 parts of collagen peptide and 2.5 parts of elastin peptide.
The preparation method of the whitening anti-aging composition based on the herbaceous plants comprises the following steps:
(1) mixing Bulbus Lilii extract, semen Persicae extract, thallus laminariae extract, and acerola cherry powder at 20 deg.C and humidity of 35% for 10min to obtain mixture A;
(2) taking 1/2 amount of the mixture A obtained in the step (1), and then premixing with collagen peptide and elastin peptide, wherein the premixing temperature is 25 ℃ to obtain premix B;
(3) and (3) mixing and stirring the rest mixture A obtained in the step (1) and the premix B obtained in the step (2) at the temperature of 30 ℃ and the humidity of 55% for 15min to obtain the whitening and anti-aging composition.
Example 3
The embodiment provides a whitening and anti-aging composition based on herbaceous plants, which comprises, by weight, 35.5 parts of lily extract, 35 parts of peach kernel extract, 20.5 parts of kelp extract, 20 parts of acerola powder, 15 parts of collagen peptide and 9.5 parts of elastin peptide.
The preparation method of the whitening anti-aging composition based on the herbaceous plants comprises the following steps:
(1) mixing Bulbus Lilii extract, semen Persicae extract, thallus laminariae extract, and acerola cherry powder at 25 deg.C and 45% humidity for 20min to obtain mixture A;
(2) taking 7/10 amount of the mixture A obtained in the step (1), and then premixing with collagen peptide and elastin peptide, wherein the premixing temperature is 30 ℃ to obtain premix B;
(3) and (3) mixing and stirring the rest mixture A obtained in the step (1) and the premix B obtained in the step (2) at the temperature of 25 ℃ and the humidity of 45% for 25min to obtain the whitening and anti-aging composition.
Example 4
This example provides a herbal-based whitening anti-aging composition, which is different from example 1 only in that the components include no elastin peptide and collagen peptide, and the components include 25 parts by weight of lily extract, 25 parts by weight of peach kernel extract, 15 parts by weight of kelp extract, and 13 parts by weight of acerola powder, and the preparation method is as in example 1.
Example 5
This example provides a herbal-based whitening anti-aging composition, which is different from example 1 only in that the composition contains no elastin peptides, and comprises, by weight, 25 parts of lily extract, 25 parts of peach kernel extract, 15 parts of kelp extract, 13 parts of acerola powder, and 15 parts of collagen peptides, and the preparation method is as in example 1.
Example 6
This example provides a herbal-based whitening anti-aging composition, which is different from example 1 only in that the composition does not include collagen peptides, and comprises, by weight, 25 parts of lily extract, 25 parts of peach kernel extract, 15 parts of kelp extract, 13 parts of acerola cherry powder, and 15 parts of elastin peptides, and the preparation method is as described in example 1.
Example 7
The present example provides a herbal-based whitening anti-aging composition, which comprises, by weight, 30 parts of lily extract, 25 parts of peach kernel extract, 15 parts of kelp extract, 14 parts of acerola powder, 10 parts of collagen peptide and 5 parts of elastin peptide, and the preparation method is as described in example 1.
Example 8
The present example provides a herbal-based whitening anti-aging composition, which comprises, by weight, 25 parts of lily extract, 28 parts of peach kernel extract, 18 parts of kelp extract, 13 parts of acerola powder, 10 parts of collagen peptide and 5 parts of elastin peptide, and the preparation method is as described in example 1.
Comparative example 1
The comparative example provides a herbal-based whitening anti-aging composition, and is different from the example 1 in that the comparative example does not contain a lily extract, the reduced amount of the lily extract is distributed into a peach kernel extract, a kelp extract and acerola powder, the proportion of the peach kernel extract, the kelp extract and the acerola powder is kept unchanged, the rest components and the component content are the same as those in the example 1, and the preparation method refers to the example 1.
Comparative example 2
The comparative example provides a herbal-based whitening anti-aging composition, and is different from example 1 in that the comparative example does not contain peach kernel extract, the reduced amount of peach kernel extract is distributed into lily extract, kelp extract and acerola powder, the proportion of the lily extract, the kelp extract and the acerola powder is kept unchanged, the rest components and the component content are the same as those in example 1, and the preparation method refers to example 1.
Comparative example 3
The comparative example provides a herbal-based whitening anti-aging composition, and is different from example 1 in that the comparative example does not contain a kelp extract, the reduced amount of the kelp extract is distributed into a peach kernel extract, a lily extract and acerola powder, the proportion of the peach kernel extract, the lily extract and the acerola powder is kept unchanged, the rest components and the component content are the same as those in example 1, and the preparation method is as in example 1.
Comparative example 4
The comparative example provides a herbal-based whitening anti-aging composition, and is different from example 1 in that acerola powder is not contained in the comparative example, the reduced amount of acerola powder is distributed into a peach kernel extract, a kelp extract and a lily extract, the proportion of the peach kernel extract, the kelp extract and the lily extract is kept unchanged, the other components and the component content are the same as those in example 1, and the preparation method is as in example 1.
Comparative example 5
The present comparative example provides a herbal-based whitening anti-aging composition, which is different from example 1 in that it does not contain peach kernel extract, kelp extract and acerola powder, and the above three components are added to lily extract in reduced amounts, the remaining components, the component contents are the same as example 1, and the preparation method is according to example 1.
Comparative example 6
The present comparative example provides a herbal-based whitening anti-aging composition, which is different from example 1 in that it does not contain lily extract, kelp extract and acerola powder, and the above three components are added to peach kernel extract in reduced amounts, the remaining components, the component contents are the same as example 1, and the preparation method is according to example 1.
Comparative example 7
The present comparative example provides a herbal-based whitening anti-aging composition, which is different from example 1 in that it does not contain lily extract, peach kernel extract and acerola powder, and the above three components are added to the kelp extract in reduced amounts, the remaining components, the component contents are the same as example 1, and the preparation method is according to example 1.
Comparative example 8
The present comparative example provides a herbal-based whitening anti-aging composition, which is different from example 1 in that it does not contain lily extract, kelp extract and peach kernel extract, and the above three components are added to acerola powder in reduced amounts, the remaining components, the component contents are the same as example 1, and the preparation method is according to example 1.
Application example 1
The application example provides a liquid beverage, and the preparation raw materials of the liquid beverage comprise, by weight: the herbal-based whitening anti-aging composition of example 1 comprises 20 parts, 15 parts of erythritol, 50 parts of purified water, 0.1 part of sweetener and 1 part of acid regulator.
The preparation method comprises the following steps:
(1) preparation: putting the whitening and anti-aging composition based on the herbaceous plants into a preparation tank, adding 50 parts of purified water, controlling the temperature to be 75 ℃, stirring at a constant temperature for 3min, then adding other residual materials, adding the residual purified water, stirring for 10min until the mixture is uniformly mixed, and then sieving by a 200-mesh sieve into a storage tank;
(2) washing the bottle: cleaning the glass bottle by using an automatic cleaning machine;
(3) filling: the liquid is separately filled by a full-automatic filling machine,
(4) and (3) sterilization: and (4) sterilizing the bottled beverage after filling by adopting a high-pressure steam sterilization pot. Sterilizing at 115 + -2 deg.C for 20min, cooling;
(5) and (4) lamp inspection: and cooling and airing the sterilized product, and performing light inspection to obtain the liquid beverage.
Application examples 2 to 6
This application example provides five liquid beverages whose formulation compositions differ from application example 1 only in that the herbal based whitening anti-aging composition of example 1 was replaced with the herbal based whitening anti-aging compositions of examples 2-6, and all other conditions were maintained. The preparation process is as in application example 1.
Comparative application examples 1 to 8
The present application example provides eight liquid beverages whose formulation compositions differ from those of application example 1 only in that the herbal-based whitening anti-aging composition of example 1 was replaced with the herbal-based whitening anti-aging compositions of comparative examples 1 to 8, and all other conditions were maintained. The preparation process is as in application example 1.
The whitening and anti-aging compositions prepared in examples 1 to 8 and comparative examples 1 to 8 were subjected to an intracellular tyrosinase inhibition test, an intracellular melanin inhibition test, an extracellular melanin transport inhibition test, a zebrafish embryo melanin inhibition test, and a skin elasticity test, and specifically tested as follows:
test example 1: intracellular tyrosinase inhibition assay
1.1 sample preparation
Diluting the sample composition with serum-free medium to a concentration of 0.45 mg/ml;
1.2 cell culture
Mouse B16 melanoma cell grows to fusion state, is digested with pancreatin-EDTA for 5min and then dispersed by blowing, the cell suspension is sucked into a centrifuge tube, is centrifuged for 5min at 1000r/min by a centrifuge, the supernatant is discarded, and a culture medium is added to prepare the cell suspension. The cell suspension is diluted to a certain concentration with the culture medium. B16 cells in logarithmic growth phase are inoculated in a 96-well plate, and the cell density is 7 multiplied by 104cfu/ml, 100. mu.L per well, was plated and labeled on the upper lid of the cell plate. Culturing is carried out in an incubator for 24 h.
After 24h of culture, the cell plates were carefully pipetted to remove the supernatant and divided into a blank group, a positive control group, and a sample group. Adding the prepared sample into the sample group, adding culture solution containing alpha-arbutin into the positive control group, and adding serum-free culture medium containing no medicine into the blank group. The addition amount is 100 mu L/hole, 4 multiple holes are made for each concentration, and after the addition is finished, the culture is carried out in an incubator for 72 hours.
1.3 determination of the intracellular tyrosinase inhibition Rate
And (3) discarding supernatant of the cell plate after incubation for 72h, adding 150 mu L of 1% TritonX-100 solution into each well, quickly freezing and storing at-80 ℃ for 30min, then thawing at room temperature to completely break the cells, pre-heating at 37 ℃ for 5min, adding 60 mu L of 0.5% L-DOPA solution, reacting at 37 ℃ for 2h, and measuring the absorbance (OD) value at 490nm of an enzyme-labeling instrument. And calculating the tyrosinase activity inhibition rate.
Tyrosinase activity inhibition (%) - (1-sample average absorbance/blank average absorbance) × 100%. The results are shown in Table 1.
Test example 2: intracellular melanin inhibition assay
2.1 sample preparation
Dissolving the sample composition with 1 × PBS to high concentration, filtering for sterilization, and diluting with cell culture solution to concentration of 0.45 mg/ml;
2.2 cell culture
Mouse B16 melanoma cell grows to fusion state, is digested with pancreatin-EDTA for 5min and then dispersed by blowing, the cell suspension is sucked into a centrifuge tube, is centrifuged for 5min at 1000r/min by a centrifuge, the supernatant is discarded, and a culture medium is added to prepare the cell suspension. The cell suspension is diluted to a certain concentration with the culture medium. Inoculating cells in logarithmic growth phase into 6-well plate at cell density of 1 × 104cfu/mL, 2mL per well, was plated and labeled on the upper lid of the cell plate. Culturing is carried out in an incubator for 24 h.
After the cells were cultured for 24 hours, the cells were divided into a sample group and a blank group. Sample group cell culture solution was changed to culture solution containing test sample, blank group was added to culture solution 2mL per well at 37 ℃ with 5% CO2Culturing for 48h in an incubator; the culture medium was changed after 48h, and 3ml of culture medium containing the sample was added to the sample group, and 5% CO was added to the blank group at 37 deg.C2Culturing for 72h in an incubator; culturing for 72 hr, changing culture solution, adding 3ml of culture solution containing sample into sample group, adding culture solution into blank group, continuing to culture at 37 deg.C and 5% CO2Culturing in an incubator for 48 h.
2.3 determination of the inhibitory Rate of intracellular Melanin
After the cell culture in 2.2 was completed, the cells were washed twice with PBS, the PBS was aspirated, and 200. mu.L of 0.25% trypsinized cells were added to each well for digestion for 5 min. The cells were blown up by adding 2ml PBS in two portions, collected in a centrifuge tube and centrifuged at 4000rpm for 5 min. The PBS residual liquid in the centrifuge tube is completely absorbed as much as possible, 200 mu L of melanin extracting solution is added into each tube, the mixture is shaken uniformly and is put into a water bath with the temperature of 80 ℃ to be heated for 1 hour. After cooling, the tube wall liquid drops are properly centrifuged and uniformly blown, 150 mu L of solution in each centrifuge tube is absorbed and transferred into a 96-well plate, and an enzyme-linked immunosorbent assay (ELIASA) detects the absorbance at 405nm of each hole.
The cell melanin inhibition (%) × (1-sample average absorbance value/blank average absorbance value) × 100%. The results are shown in Table 1.
Test example 3: extracellular melanin transport inhibition assay
3.1 sample preparation
Dissolving the sample composition with 1 × PBS to high concentration, filtering for sterilization, and diluting with cell culture solution to concentration of 0.45 mg/ml;
3.2 cell culture
Mouse B16 melanoma cell grows to fusion state, is digested with pancreatin-EDTA for 5min and then dispersed by blowing, the cell suspension is sucked into a centrifuge tube, is centrifuged for 5min at 1000r/min by a centrifuge, the supernatant is discarded, and a culture medium is added to prepare the cell suspension. The cell suspension is diluted to a certain concentration with the culture medium. Inoculating cells in logarithmic growth phase into 6-well plate at cell density of 1 × 104cfu/mL, 2mL per well, was plated and labeled on the upper lid of the cell plate. Culturing is carried out in an incubator for 24 h.
After the cells were cultured for 24 hours, the cells were divided into a sample group and a blank group. Sample group cell culture solution was changed to culture solution containing test sample, blank group was added to culture solution 2mL per well at 37 ℃ with 5% CO2Culturing for 48h in an incubator; the culture medium was changed after 48h, and 3ml of culture medium containing the sample was added to the sample group, and 5% CO was added to the blank group at 37 deg.C2Culturing for 72h in an incubator; culturing for 72 hr, changing culture solution, adding 3ml of culture solution containing sample into sample group, adding culture solution into blank group, continuing to culture at 37 deg.C and 5% CO2Culturing in an incubator for 48 h.
3.3 detection of extracellular melanin content
After the cell culture in 3.2 is finished, completely absorbing the culture solution in the culture medium to the greatest extent in a centrifuge tube, adding 200 mu L of melanin extracting solution into each tube, shaking uniformly, and heating in a water bath at 80 ℃ for 1 h. After cooling, the tube wall liquid drops are properly centrifuged and uniformly blown, 150 mu L of solution in each centrifuge tube is absorbed and transferred into a 96-well plate, and an enzyme-linked immunosorbent assay (ELIASA) detects the absorbance at 405nm of each hole.
Extracellular melanin transport inhibition (%) × (1-sample average absorbance value/blank average absorbance value) × 100%. The results are shown in Table 1.
Test example 4: zebra fish embryo melanin inhibition assay
4.1 sample preparation
Preparation of a test substance: the test composition was directly dissolved in fish embryo culture solution to prepare a test solution with a concentration of 1 mg/ml.
4.2 culture and detection
Selecting healthy zebra fish embryos, and culturing at the density of no more than 1 fish embryo in 200 mu L of fish embryo culture solution at 28 ℃ until the fertilized time is 6-8 h. The 96-well plate is divided into 3 groups, namely a blank control group (fish embryo culture solution), a positive control group (phenylthiourea working solution) and a test object group (test object), and corresponding fish embryo culture solution is added. Subsequently, 24 fish embryos multiplied by 3 are randomly selected to be put into a 96-well plate and placed in an incubator at 28 +/-1 ℃ to be cultured for 48 +/-1 h after fertilization. Covering the fish embryos with 2-4% of methyl cellulose, and taking pictures of the fish embryos under a stereoscopic microscope according to uniform shooting parameters. The head and yolk back regions of each fish embryo were labeled with analytical software, and the "average intensity of measurement" in the "measurement" column was selected, and the "average signal intensity" in the test results was selected as the melanin signal intensity. And calculating the melanin inhibition rate.
The inhibition ratio of the zebra fish embryo melanin is [ (the level of the normal zebra fish embryo melanin-the level of the zebra fish embryo melanin in a sample treatment group) × 100/(the level of the normal zebra fish embryo melanin-the level of the zebra fish embryo melanin in a positive control group) ]%. The results are shown in Table 1.
TABLE 1
The data of the examples 1 to 3 show that the whitening and anti-aging composition based on the herbaceous plants provided by the invention has the intracellular tyrosinase inhibition rate of 34.63% -37.68%, the intracellular melanin inhibition rate of 24.26% -27.06%, the extracellular melanin transport inhibition rate of 19.35% -21.63% and the zebra fish embryo melanin inhibition rate of 34.63% -40%, which indicates that the composition has significant whitening and anti-aging and skin-health effects on human skin; as can be seen from examples 1 and 4 to 6, when collagen peptide and elastin peptide are added to the composition provided by the present invention, the intracellular tyrosinase inhibition rate, the intracellular melanin inhibition rate, the extracellular melanin transport inhibition rate, and the zebra fish embryo melanin inhibition rate of the composition can reach the highest, and the effect is the best; as can be seen by comparing examples 2-3 with examples 7-8, the range of further preferred components of the present invention, the effect is better relative to the effect outside the preferred range; as can be seen from comparison of example 1 with comparative examples 1 to 8, when any one of lily extract, peach kernel extract, kelp extract and acerola powder is absent from the composition, the intracellular tyrosinase inhibition rate, the intracellular melanin inhibition rate, the extracellular melanin transport inhibition rate, and the zebrafish embryo melanin inhibition rate of the composition are significantly decreased.
Test example 5: skin moisture improving and firming skin test
The liquid beverages prepared in application examples 1 to 6 and comparative application examples 1 to 8 were evaluated for skin moisture improving and skin firming effects:
130 subjects of 20-45 years old were randomly selected and divided into 3 groups of 10. The liquid beverages prepared in application examples 1-3, application examples 4-6 and comparative application examples 1-8 were drunk by 13 groups of test subjects, 150mL was drunk daily for 30 days, and the test subjects were not allowed to take other articles for keeping skin moisture and skin elasticity and cosmetics for influencing result determination during the test period without changing the original dietary habits during the test period.
The skin moisture content was measured by a skin moisture content tester Corneometer CM825 on day 0 and day 30, respectively, and the moisture content of the skin before and after drinking was evaluated by repeating the measurement 3 times with the probe in the same area to be measured, and the average value was taken. The results are shown in Table 2.
The skin condition on day 0 and day 30 was measured using the skin elastometer Cutomerma 580, respectively, the test site was the cheekbone, the probe repeated 3 times in the same test area during the measurement, and the average was taken. The test parameter is R2 (the ratio of the springback amount Ua of the skin without negative pressure to the maximum stretch amount Uf with negative pressure), the closer R2 is to 1, the better the elasticity of the skin, the average change value of R2 is R2 on day 30 to R2 on day 0, and the average change rate of R2 is R2/R2 on day 0. The results of the specific skin elasticity tests are shown in table 2.
TABLE 2
| Group of | Moisture content increase rate (%) | Average rate of change of skin elasticity R2 |
| Application example 1 | 10.59％ | 12.05％ |
| Application example 2 | 9.66％ | 11.83％ |
| Application example 3 | 9.85％ | 11.90％ |
| Application example 4 | 5.09％ | 6.34％ |
| Application example 5 | 6.67％ | 7.28％ |
| Application example 6 | 6.09％ | 7.34％ |
| Comparative application example 1 | 8.67％ | 9.68％ |
| Comparative application example 2 | 8.54％ | 9.75％ |
| Comparative application example 3 | 8.92％ | 9.93％ |
| Comparative application example 4 | 8.45％ | 8.53％ |
| Comparative application example 5 | 7.84％ | 7.17％ |
| Comparative application example 6 | 7.74％ | 8.28％ |
| Comparative application example 7 | 7.63％ | 8.42％ |
| Comparative application example 8 | 7.15％ | 8.69％ |
The results show that: the herbal-based whitening and anti-aging composition and the prepared beverage have good skin moisture improving and skin tightening effects, wherein six components, namely lily extract, peach kernel extract, kelp extract, acerola powder, collagen peptide and elastin peptide, are mutually promoted in the effect, and have unexpected synergistic effect.
In conclusion, the whitening and anti-aging composition based on the herbaceous plants is scientific and reasonable in proportion through selection of six active functional components and a large number of experimental demonstrations, the whitening and anti-aging effects of the composition are synergistically enhanced, and the components are mainly herbaceous plant components, so that the safety, the effectiveness and the skin feeling of the composition are improved. The invention can bring good economic benefit for enterprises and has wide application prospect.
The applicant states that the present invention is illustrated by the above examples, but the present invention is not limited to the above detailed methods, i.e., it does not mean that the present invention must be implemented by relying on the above detailed methods. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (10)
1. A herbal-based whitening anti-aging composition is characterized by comprising a lily extract, a peach kernel extract, a kelp extract and acerola powder.
2. The herbal-based whitening anti-aging composition according to claim 1, comprising 15.5-35.5 parts by weight of lily extract, 15-35 parts by weight of peach kernel extract, 10.5-20.5 parts by weight of kelp extract and 9.5-20 parts by weight of acerola powder.
3. The herbal-based whitening anti-aging composition according to claim 1 or 2, wherein the components of the herbal-based whitening anti-aging composition further comprise collagen peptides and/or elastin peptides;
preferably, the collagen peptide is 3-15 parts by weight;
preferably, the weight part of the elastin peptide is 2.5-9.5 parts;
preferably, the herbal plant-based whitening anti-aging composition comprises 25-30 parts by weight of lily extract, 25-30 parts by weight of peach kernel extract, 15-18 parts by weight of kelp extract, 12-15 parts by weight of acerola powder, 3-15 parts by weight of collagen peptide and 2.5-9.5 parts by weight of elastin peptide.
4. The preparation method of the herbal-based whitening anti-aging composition according to any one of claims 1 to 3, wherein the preparation method comprises: mixing and stirring uniformly lily extract, peach kernel extract, kelp extract and acerola powder according to the formula amount to obtain the whitening anti-aging composition.
5. The preparation method of the herbal-based whitening anti-aging composition according to claim 4, wherein the method comprises the steps of:
(1) firstly, mixing and stirring lily extract, peach kernel extract, kelp extract and acerola cherry powder uniformly according to the formula amount to obtain a mixture A;
(2) premixing collagen peptide and elastin peptide with part of the mixture A obtained in the step (1) to obtain a premix B;
(3) uniformly mixing and stirring the premix B obtained in the step (2) and the rest mixture A obtained in the step (1) to obtain the whitening and anti-aging composition;
preferably, the stirring temperature in the step (1) is 20-25 ℃, and the stirring time is 10-20 min;
preferably, the humidity is controlled to be 35% -45% during mixing and stirring in the step (1);
preferably, the temperature of the pre-mixing in the step (2) is 20-30 ℃;
preferably, the part of the mixture A obtained in the step (1) in the step (2) accounts for 50 to 70 percent of the total amount of the mixture A obtained in the step (1);
preferably, the stirring temperature in the step (3) is 25-30 ℃, and the stirring time is 15-25 min;
preferably, the humidity is controlled to be 45% -55% during the mixing and stirring in the step (3).
6. Use of the herbal-based whitening anti-aging composition according to any one of claims 1 to 3 in foods, health products or cosmetics;
preferably, the food product comprises a tabletted candy, a gel candy, a liquid beverage or a solid beverage.
7. The tablet candy is characterized by comprising the following preparation raw materials in parts by weight: the herbal-based whitening anti-aging composition according to any one of claims 1 to 3, 30 to 60 parts, 10 to 17 parts of microcrystalline cellulose, 15 to 28 parts of isomalt, 0.01 to 1 part of sodium carboxymethylcellulose, 1 to 5 parts of silicon dioxide, and 1 to 5 parts of magnesium stearate.
8. The gel candy is characterized by comprising the following preparation raw materials in parts by weight: 2-15 parts of the herbal-based whitening anti-aging composition according to any one of claims 1-3, 3-10 parts of a gel, 40-60 parts of a sweetener, 1-4 parts of a collagen casing, 3-7 parts of a fruit juice concentrate, 0.2-0.3 part of a flavoring essence, and 8-30 parts of purified water.
9. The liquid beverage is characterized in that the preparation raw materials of the liquid beverage comprise, by weight: the herbal-based whitening anti-aging composition according to any one of claims 1 to 3, 10 to 30 parts, erythritol 10 to 20 parts, purified water 30 to 60 parts, sweetener 0.01 to 0.2 parts, and acid modulator 0.01 to 4 parts.
10. The solid beverage is characterized in that the solid beverage is prepared from the following raw materials in parts by weight: 30-60 parts of the herbal-based whitening anti-aging composition according to any one of claims 1-3, 0.01-1 part of sucralose, 0.01-4.5 parts of flavoring essence and 15-50 parts of resistant dextrin.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114521652A (en) * | 2022-02-22 | 2022-05-24 | 拜斯特药业(广州)有限公司 | Composition with anti-aging effect and application thereof |
| CN115444086A (en) * | 2022-09-23 | 2022-12-09 | 浙江中医药大学 | Health-care beverage with whitening and skin-brightening functions and preparation method and application thereof |
| CN116869167A (en) * | 2023-08-15 | 2023-10-13 | 拜斯特药业(广州)有限公司 | Composite polysaccharide composition and preparation method and application thereof |
| CN117297992A (en) * | 2023-08-16 | 2023-12-29 | 宝萃生物科技有限公司 | Whitening composition and preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011016760A (en) * | 2009-07-09 | 2011-01-27 | Noevir Co Ltd | Skin care external preparation, oral agent, moisturizing agent, anti-aging agent, bleaching agent, and anti-oxidizing agent containing bulb of lilium plant and/or callus extract as effective ingredient |
| WO2011149259A2 (en) * | 2010-05-28 | 2011-12-01 | 경희대학교 산학협력단 | Composition including compound derived from peach kernel for treating hyperpigmentation skin disorder and whitening skin |
| US20120195923A1 (en) * | 2009-06-17 | 2012-08-02 | Universite Laval | Use of skin care compositions comprising laminariacea extract for treatment of skin aging signs |
| CN107549812A (en) * | 2017-10-16 | 2018-01-09 | 海南三元星生物科技股份有限公司 | It is a kind of using acerola concentrate extract, haematococcus pluvialis and Isin glue collagen peptide as beautifying and anti-aging food of raw material and preparation method thereof |
| CN109156829A (en) * | 2018-09-12 | 2019-01-08 | 广州市顺芝堂生物科技有限公司 | A kind of Isin glue collagen composition and its preparation method and application |
| CN111329808A (en) * | 2020-04-03 | 2020-06-26 | 西安力邦医美科技有限公司 | Cosmetic composition with anti-glycosylation and anti-aging effects and application thereof |
| CN113648262A (en) * | 2021-08-26 | 2021-11-16 | 北京工商大学 | Lily leaf and cherry fermented product for cosmetic and preparation method thereof |
-
2021
- 2021-12-02 CN CN202111459582.6A patent/CN113975217B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120195923A1 (en) * | 2009-06-17 | 2012-08-02 | Universite Laval | Use of skin care compositions comprising laminariacea extract for treatment of skin aging signs |
| JP2011016760A (en) * | 2009-07-09 | 2011-01-27 | Noevir Co Ltd | Skin care external preparation, oral agent, moisturizing agent, anti-aging agent, bleaching agent, and anti-oxidizing agent containing bulb of lilium plant and/or callus extract as effective ingredient |
| WO2011149259A2 (en) * | 2010-05-28 | 2011-12-01 | 경희대학교 산학협력단 | Composition including compound derived from peach kernel for treating hyperpigmentation skin disorder and whitening skin |
| CN107549812A (en) * | 2017-10-16 | 2018-01-09 | 海南三元星生物科技股份有限公司 | It is a kind of using acerola concentrate extract, haematococcus pluvialis and Isin glue collagen peptide as beautifying and anti-aging food of raw material and preparation method thereof |
| CN109156829A (en) * | 2018-09-12 | 2019-01-08 | 广州市顺芝堂生物科技有限公司 | A kind of Isin glue collagen composition and its preparation method and application |
| CN111329808A (en) * | 2020-04-03 | 2020-06-26 | 西安力邦医美科技有限公司 | Cosmetic composition with anti-glycosylation and anti-aging effects and application thereof |
| CN113648262A (en) * | 2021-08-26 | 2021-11-16 | 北京工商大学 | Lily leaf and cherry fermented product for cosmetic and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114521652A (en) * | 2022-02-22 | 2022-05-24 | 拜斯特药业(广州)有限公司 | Composition with anti-aging effect and application thereof |
| CN115444086A (en) * | 2022-09-23 | 2022-12-09 | 浙江中医药大学 | Health-care beverage with whitening and skin-brightening functions and preparation method and application thereof |
| CN116869167A (en) * | 2023-08-15 | 2023-10-13 | 拜斯特药业(广州)有限公司 | Composite polysaccharide composition and preparation method and application thereof |
| CN117297992A (en) * | 2023-08-16 | 2023-12-29 | 宝萃生物科技有限公司 | Whitening composition and preparation method and application thereof |
| CN117297992B (en) * | 2023-08-16 | 2024-04-26 | 宝萃生物科技有限公司 | Whitening composition and preparation method and application thereof |
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| CN113975217B (en) | 2022-10-21 |
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