CN113974202B - Environment-friendly electronic atomized liquid thickener and preparation method thereof - Google Patents
Environment-friendly electronic atomized liquid thickener and preparation method thereof Download PDFInfo
- Publication number
- CN113974202B CN113974202B CN202111243140.8A CN202111243140A CN113974202B CN 113974202 B CN113974202 B CN 113974202B CN 202111243140 A CN202111243140 A CN 202111243140A CN 113974202 B CN113974202 B CN 113974202B
- Authority
- CN
- China
- Prior art keywords
- seed
- atomized liquid
- enzymolysis
- thickener
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/10—Chemical features of tobacco products or tobacco substitutes
- A24B15/16—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/10—Chemical features of tobacco products or tobacco substitutes
- A24B15/16—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes
- A24B15/167—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes in liquid or vaporisable form, e.g. liquid compositions for electronic cigarettes
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
Abstract
The invention provides an environment-friendly electronic atomized liquid thickener and a preparation method thereof. The thickener has the advantages of environment-friendly raw materials, high viscosity and low volatility, and the main components of the thickener comprise a plurality of polysaccharide components such as galactomannan, konjac glucomannan, pullulan and the like, and can be used for preparing electronic atomized liquid, and the obtained electronic atomized liquid has better use experience.
Description
Technical Field
The invention relates to the technical field of electronic atomized liquid processing, in particular to an environment-friendly electronic atomized liquid thickener and a preparation method thereof.
Background
The traditional cigarette is ignited and burned by tobacco leaves or tobacco, so that smoke is generated, the health of smokers and non-smokers is simultaneously endangered, and various respiratory diseases, such as tracheitis, bronchitis, pharyngitis, cough, excessive phlegm and the like, are easily caused. In recent years, along with increasing importance of smoking and health relationship in society, smoke control actions have come up in many places, and the public places are fully prohibited from producing smoke regulations, but many smokers cannot stop smoking, so people replace cigarettes with electronic cigarettes in order to reduce harm of smoking.
The electronic cigarette is characterized in that a solution containing tobacco extracts and tobacco essence is atomized into particles, the particles are absorbed by lungs, and simulated smoke is simultaneously spit out, so that the process is similar to that of smoking a real cigarette, no second-hand smoke is generated, and the second-hand smoke is not scattered or wrapped in a closed space, and other harmful components such as tar, suspended particles and the like in the traditional cigarette are avoided.
Viscosity is an important index of the electronic cigarette atomized liquid. The electronic atomized liquid has low viscosity and good fluidity, but is liable to cause leakage. The traditional electronic atomized liquid has the problem of leakage caused by low viscosity of the electronic atomized liquid. In addition, the electronic atomization liquid is unstable in property and high in volatilization rate, fragrance cannot be locked, and use experience of the electronic cigarette can be affected.
Disclosure of Invention
The invention aims to provide an environment-friendly electronic atomized liquid thickener and a preparation method thereof, which have the advantages of high viscosity, low volatilization rate and better use experience.
In order to achieve the above purpose, the invention is realized by the following scheme:
the preparation method of the environment-friendly electronic atomized liquid thickener comprises the following specific steps:
(1) Removing impurities and stones from the fenugreek seeds, peeling, adding water for tempering, and crushing to 20-30 meshes to obtain fenugreek seed powder;
(2) Slicing fresh konjak, soaking in saturated lime water, filtering, pulverizing into colloidal substance by a refiner, adding water, shearing to obtain slurry, adding cellulase and alpha-amylase into the slurry, and performing enzymolysis to obtain a pre-enzymolysis product;
(3) Culturing Aureobasidium pullulans by secondary seeds to obtain seed liquid, and inoculating the seed liquid into a fermentation medium containing sucrose for culturing to obtain pullulan fermentation liquor;
(4) And finally, adding the fenugreek seed powder obtained in the step (1) and the pre-enzymolysis product obtained in the step (2) into the pullulan fermentation broth obtained in the step (3), then adding lipase, cellulase and neutral protease, carrying out enzymolysis, and carrying out post-treatment to obtain the thickener.
Preferably, in the step (1), the concrete method of water adding tempering comprises the following steps: adding water with the weight of 0.02-0.03 times of that of the peeled fenugreek seeds, and carrying out ultrasonic oscillation for 5-7 minutes at the weight of 300-400W.
Preferably, in the step (2), the saturated lime water soaking time is 40-50 minutes.
Preferably, in the step (2), the shearing process conditions are as follows: shearing for 10-15 min at 1500-2500 r/min.
Preferably, in the step (2), the mass ratio of the fresh konjak to the saturated lime water to the cellulase to the alpha-amylase is 300-320: 650-680: 600-700: 2-3: 1.5 to 2.
Preferably, in the step (2), the enzymolysis process conditions are as follows: pH=5-6, and enzymolysis at 45-50 deg.c for 2-3 hr.
Preferably, the specific method of step (3) is as follows: inoculating Aureobasidium pullulans into a seed culture medium, culturing for 22-25 hours at the temperature of 27-29 ℃ through a primary seed tank, inoculating into a secondary seed tank according to the inoculum size of 8-12% by volume, and culturing for 22-25 hours at the temperature of 27-29 ℃ to obtain seed liquid; and then inoculating the seed solution into a fermentation medium according to the inoculum size of 4-6% by volume, and culturing for 52-58 hours at the temperature of 27-29 ℃ to obtain the pullulan fermentation broth.
Further preferably, the seed medium composition is: sucrose 50.0g/L, yeast extract 1.9g/L, dipotassium hydrogen phosphate 6.1g/L, sodium chloride 0.9g/L, magnesium sulfate 0.2g/L, ammonium sulfate 0.5g/L, adjust pH=6.5, and sterilize at 121 ℃ for 20 minutes.
Further preferably, the composition of the fermentation medium is: sucrose 100.0g/L, yeast extract 1.9g/L, ammonium sulfate 1.6g/L, magnesium sulfate 0.6g/L, dipotassium phosphate 4.8g/L, methionine 0.5g/L, aspartic acid 0.5g/L, valine 0.5g/L, glutamic acid 0.5g/L, defoamer (GPE 20) 0.1g/L, pH=6.5, sterilization at 121℃for 20 minutes.
Preferably, in the step (4), the mass ratio of the fenugreek seed powder to the pre-enzymolysis product to the pullulan fermentation broth to the lipase to the cellulase to the neutral protease is 0.1-0.2: 80-90: 20-30: 0.08 to 0.1:0.1 to 0.12: 0.06-0.08.
Preferably, in the step (4), the enzymolysis process conditions are as follows: enzymolysis is carried out for 80-100 minutes at 40-45 ℃.
Preferably, in the step (4), the specific method of the post-treatment comprises the following steps in parts by weight: ultrafiltering the enzymolysis product with 10000Da ultrafilter membrane, concentrating to half of the original volume to obtain concentrated solution, adding 0.1-0.2 part of sodium chloride into 100 parts of concentrated solution, adding 10-15 parts of absolute ethyl alcohol, standing at 0-4 ℃ for 24-36 hours, centrifuging to obtain precipitate, slowly adding the precipitate into 250-300 parts of distilled water while stirring, fully stirring, centrifuging to remove insoluble matters, and obtaining the thickener.
The environment-friendly electronic atomized liquid thickener is prepared by the preparation method.
The environment-friendly electronic atomized liquid thickener is applied to the preparation of electronic atomized liquid.
Compared with the prior art, the invention has the beneficial effects that:
the invention adds fenugreek seed powder and pre-enzymolysis product into pullulan fermentation broth, then adds lipase, cellulase and neutral protease, carries out enzymolysis and post-treatment to prepare the thickener. The thickener has the advantages of environment-friendly raw materials, high viscosity and low volatility, and the main components of the thickener comprise a plurality of polysaccharide components such as galactomannan, konjac glucomannan, pullulan and the like, and can be used for preparing electronic atomized liquid, and the obtained electronic atomized liquid has better use experience.
The invention removes impurities and stones from the fenugreek seeds, peels off, adds water for tempering, breaks the fenugreek seeds to 20 to 30 meshes to obtain fenugreek seed powder, and releases polysaccharide such as galactomannan and the like after the fenugreek seed powder is added into pullulan fermentation liquor for enzymolysis treatment, contains abundant hydroxyl groups, can form hydrogen bonds with water and base oil, and plays a role in thickening through hydrogen bonding.
Slicing fresh konjak, soaking in saturated lime water, filtering, pulverizing into gelatinous substance by a refiner, adding water, shearing to obtain slurry, adding cellulase and alpha-amylase into the slurry, and performing enzymolysis to obtain pre-enzymolysis product which contains polysaccharide such as konjak gum and dietary fiber. The pullulan contained in the pullulan fermentation liquor also has a thickening effect, and the pullulan is mixed with the fenugreek seed powder and the pre-enzymolysis product for enzymolysis, so that the thickening effect is synergistically improved.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The preparation method of the environment-friendly electronic atomized liquid thickener comprises the following specific steps:
(1) Removing impurities and stones from the fenugreek seeds, peeling, adding water for tempering, and crushing to 20 meshes to obtain fenugreek seed powder;
(2) Then slicing 320g of fresh konjak, adding 650g of saturated lime water, soaking for 50 minutes, filtering, crushing into colloidal substances by a refiner, adding 600g of water, shearing for 10 minutes at 2500r/min to obtain slurry, adding 3g of cellulase and 1.5g of alpha-amylase into the slurry, regulating pH to be 6, and carrying out enzymolysis at 45 ℃ for 3 hours to obtain a pre-enzymolysis product;
(3) Inoculating Aureobasidium pullulans into a seed culture medium, culturing for 25 hours at 27 ℃ through a primary seed tank, and then inoculating into a secondary seed tank according to the inoculum size of 8% by volume, and culturing for 22 hours at 27 ℃ to obtain seed liquid; then inoculating the seed solution into a fermentation medium according to the inoculum size of 6% by volume, and culturing at 27 ℃ for 58 hours to obtain pullulan fermentation liquor;
(4) Finally, adding 0.1g of fenugreek seed powder and 90g of pre-enzymolysis product into 20g of pullulan fermentation liquor, then adding 0.1g of lipase, 0.1g of cellulase and 0.08g of neutral protease, carrying out enzymolysis for 100 minutes at 40 ℃, and carrying out post-treatment to obtain the thickener.
In the step (1), the concrete method of water adding tempering comprises the following steps: adding 0.02 times of water into peeled semen Trigonellae, and oscillating with 400W ultrasonic wave for 5 min.
In the step (3), the seed culture medium comprises the following components: sucrose 50.0g/L, yeast extract 1.9g/L, dipotassium hydrogen phosphate 6.1g/L, sodium chloride 0.9g/L, magnesium sulfate 0.2g/L, ammonium sulfate 0.5g/L, adjust pH=6.5, and sterilize at 121 ℃ for 20 minutes.
The composition of the fermentation medium is: sucrose 100.0g/L, yeast extract 1.9g/L, ammonium sulfate 1.6g/L, magnesium sulfate 0.6g/L, dipotassium phosphate 4.8g/L, methionine 0.5g/L, aspartic acid 0.5g/L, valine 0.5g/L, glutamic acid 0.5g/L, defoamer (GPE 20) 0.1g/L, pH=6.5, sterilization at 121℃for 20 minutes.
In the step (4), the specific method of post-treatment is as follows: ultrafiltering the enzymolysis product with 10000Da ultrafilter membrane, concentrating to half of the original volume to obtain concentrated solution, adding 0.2g sodium chloride into 100g concentrated solution, adding 10g absolute ethyl alcohol, standing at 4deg.C for 24 hr, centrifuging to obtain precipitate, slowly adding the precipitate into 300g distilled water while stirring, stirring thoroughly, centrifuging to remove insoluble substances, and obtaining the thickener.
Example 2
The preparation method of the environment-friendly electronic atomized liquid thickener comprises the following specific steps:
(1) Removing impurities and stones from the fenugreek seeds, peeling, adding water for tempering, and crushing to 30 meshes to obtain fenugreek seed powder;
(2) Then 300g of fresh konjak is sliced, is added into 680g of saturated lime water for soaking for 40 minutes, is filtered, is crushed into gelatinous substances by a refiner, 700g of water is added, is sheared for 15 minutes at 1500r/min to obtain slurry, then 2g of cellulase and 2g of alpha-amylase are added into the slurry, pH=5 is adjusted, and enzymolysis is carried out for 2 hours at 50 ℃ to obtain a pre-enzymolysis product;
(3) Inoculating Aureobasidium pullulans into a seed culture medium, culturing for 22 hours at 29 ℃ through a first-stage seed tank, and then inoculating into a second-stage seed tank according to 12% of the volume of inoculation, and culturing for 25 hours at 29 ℃ to obtain seed liquid; then inoculating the seed solution into a fermentation medium according to the inoculation amount of 4% by volume, and culturing for 52 hours at 29 ℃ to obtain pullulan fermentation liquor;
(4) Finally, adding 0.2g of fenugreek seed powder and 80g of pre-enzymolysis product into 30g of pullulan fermentation liquor, then adding 0.08g of lipase, 0.12g of cellulase and 0.06g of neutral protease, carrying out enzymolysis for 80 minutes at 45 ℃, and carrying out post-treatment to obtain the thickener.
In the step (1), the concrete method of water adding tempering comprises the following steps: the peeled fenugreek seeds were added with 0.03 times of water by weight, and subjected to ultrasonic vibration at 300W for 7 minutes.
In the step (3), the seed culture medium comprises the following components: sucrose 50.0g/L, yeast extract 1.9g/L, dipotassium hydrogen phosphate 6.1g/L, sodium chloride 0.9g/L, magnesium sulfate 0.2g/L, ammonium sulfate 0.5g/L, adjust pH=6.5, and sterilize at 121 ℃ for 20 minutes.
The composition of the fermentation medium is: sucrose 100.0g/L, yeast extract 1.9g/L, ammonium sulfate 1.6g/L, magnesium sulfate 0.6g/L, dipotassium phosphate 4.8g/L, methionine 0.5g/L, aspartic acid 0.5g/L, valine 0.5g/L, glutamic acid 0.5g/L, defoamer (GPE 20) 0.1g/L, pH=6.5, sterilization at 121℃for 20 minutes.
In the step (4), the specific method of post-treatment is as follows: ultrafiltering the enzymolysis product with 10000Da ultrafilter membrane, concentrating to half of the original volume to obtain concentrated solution, adding 0.1g sodium chloride into 100g concentrated solution, adding 15g absolute ethyl alcohol, standing at 0deg.C for 36 hr, centrifuging to obtain precipitate, slowly adding the precipitate into 250g distilled water while stirring, stirring thoroughly, centrifuging to remove insoluble substances, and obtaining the thickener.
Example 3
The preparation method of the environment-friendly electronic atomized liquid thickener comprises the following specific steps:
(1) Removing impurities and stones from the fenugreek seeds, peeling, adding water for tempering, and crushing to 30 meshes to obtain fenugreek seed powder;
(2) Then slicing 310g of fresh konjak, adding 660g of saturated lime water, soaking for 45 minutes, filtering, crushing into colloidal substances by a refiner, adding 650g of water, shearing for 12 minutes at 2000r/min to obtain slurry, adding 2.5g of cellulase and 1.8g of alpha-amylase into the slurry, regulating pH to be 5, and carrying out enzymolysis at 48 ℃ for 2.5 hours to obtain a pre-enzymolysis product;
(3) Inoculating Aureobasidium pullulans into a seed culture medium, culturing for 23 hours at 28 ℃ through a primary seed tank, and then inoculating into a secondary seed tank according to 10% of the volume of inoculation, and culturing for 24 hours at 28 ℃ to obtain seed liquid; then inoculating the seed solution into a fermentation medium according to the inoculum size of 5% by volume, and culturing at 28 ℃ for 55 hours to obtain pullulan fermentation liquor;
(4) Finally, adding 0.15g of fenugreek seed powder and 85g of pre-enzymolysis product into 25g of pullulan fermentation liquor, then adding 0.09g of lipase, 0.11g of cellulase and 0.07g of neutral protease, carrying out enzymolysis for 90 minutes at 42 ℃, and carrying out post-treatment to obtain the thickener.
In the step (1), the concrete method of water adding tempering comprises the following steps: the peeled fenugreek seeds were added with 0.025 times of water by weight, and subjected to ultrasonic vibration at 400W for 6 minutes.
In the step (3), the seed culture medium comprises the following components: sucrose 50.0g/L, yeast extract 1.9g/L, dipotassium hydrogen phosphate 6.1g/L, sodium chloride 0.9g/L, magnesium sulfate 0.2g/L, ammonium sulfate 0.5g/L, adjust pH=6.5, and sterilize at 121 ℃ for 20 minutes.
The composition of the fermentation medium is: sucrose 100.0g/L, yeast extract 1.9g/L, ammonium sulfate 1.6g/L, magnesium sulfate 0.6g/L, dipotassium phosphate 4.8g/L, methionine 0.5g/L, aspartic acid 0.5g/L, valine 0.5g/L, glutamic acid 0.5g/L, defoamer (GPE 20) 0.1g/L, pH=6.5, sterilization at 121℃for 20 minutes.
In the step (4), the specific method of post-treatment is as follows: ultrafiltering the enzymolysis product with 10000Da ultrafilter membrane, concentrating to half of the original volume to obtain concentrated solution, adding 0.15g sodium chloride into 100g concentrated solution, adding 12g absolute ethyl alcohol, standing at 2deg.C for 32 hr, centrifuging to obtain precipitate, slowly adding the precipitate into 270g distilled water while stirring, stirring thoroughly, centrifuging to remove insoluble substances, and obtaining the thickener.
Comparative example
The preparation method of the environment-friendly electronic atomized liquid thickener comprises the following specific steps:
(1) Firstly, slicing 320g of fresh konjak, adding 650g of saturated lime water, soaking for 50 minutes, filtering, crushing into colloidal substances by a refiner, adding 600g of water, shearing for 10 minutes at 2500r/min to obtain slurry, then adding 3g of cellulase and 1.5g of alpha-amylase into the slurry, regulating pH to be 6, and carrying out enzymolysis at 45 ℃ for 3 hours to obtain a pre-enzymolysis product;
(2) Inoculating Aureobasidium pullulans into a seed culture medium, culturing for 25 hours at 27 ℃ through a primary seed tank, and then inoculating into a secondary seed tank according to the inoculum size of 8% by volume, and culturing for 22 hours at 27 ℃ to obtain seed liquid; then inoculating the seed solution into a fermentation medium according to the inoculum size of 6% by volume, and culturing at 27 ℃ for 58 hours to obtain pullulan fermentation liquor;
(3) Finally, adding 90g of the pre-enzymolysis product into 20g of pullulan fermentation liquor, then adding 0.1g of lipase, 0.1g of cellulase and 0.08g of neutral protease, carrying out enzymolysis for 100 minutes at 40 ℃, and carrying out post-treatment to obtain the thickener.
In the step (2), the seed culture medium consists of: sucrose 50.0g/L, yeast extract 1.9g/L, dipotassium hydrogen phosphate 6.1g/L, sodium chloride 0.9g/L, magnesium sulfate 0.2g/L, ammonium sulfate 0.5g/L, adjust pH=6.5, and sterilize at 121 ℃ for 20 minutes.
The composition of the fermentation medium is: sucrose 100.0g/L, yeast extract 1.9g/L, ammonium sulfate 1.6g/L, magnesium sulfate 0.6g/L, dipotassium phosphate 4.8g/L, methionine 0.5g/L, aspartic acid 0.5g/L, valine 0.5g/L, glutamic acid 0.5g/L, defoamer (GPE 20) 0.1g/L, pH=6.5, sterilization at 121℃for 20 minutes.
In the step (3), the specific method of post-treatment is as follows: ultrafiltering the enzymolysis product with 10000Da ultrafilter membrane, concentrating to half of the original volume to obtain concentrated solution, adding 0.2g sodium chloride into 100g concentrated solution, adding 10g absolute ethyl alcohol, standing at 4deg.C for 24 hr, centrifuging to obtain precipitate, slowly adding the precipitate into 300g distilled water while stirring, stirring thoroughly, centrifuging to remove insoluble substances, and obtaining the thickener.
The thickeners obtained in examples 1 to 3 and comparative example were used for the preparation of an electronic atomized liquid, respectively, and the specific method is as follows: 70g of propylene glycol and 30g of glycerol are stirred and mixed uniformly, then 1g of thickener is added, and the mixture is stirred and mixed uniformly, thus obtaining the electronic atomized liquid. Measuring the viscosity of the electronic atomized liquid using a rotary viscometer (NDJ-1, commercially available from Shanghai Ping Xuan scientific instrument); standing 100g of electronic atomized liquid at normal temperature for 30 days, testing the mass before and after standing, and calculating the volatilization rate, wherein the volatilization rate is = (100-mass after standing)/100×100%; the test results are shown in Table 1.
TABLE 1 electronic atomized liquid Performance test
As can be seen from Table 1, the thickeners obtained in examples 1 to 3 have a good thickening effect on the electronic atomized liquid, and can significantly reduce the volatilization rate and improve the use experience of the electronic atomized liquid.
According to the comparative example, the fenugreek seeds are omitted, the viscosity of the electronic atomized liquid prepared from the obtained thickener is low, the volatility is high, and the synergistic effect of all components in the fenugreek seeds, konjak, pullulan fermentation liquor and the like is shown, so that the thickening effect is achieved, the volatility of the electronic atomized liquid is reduced, and the use experience of the electronic atomized liquid is better.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (8)
1. The preparation method of the environment-friendly electronic atomized liquid thickener is characterized by comprising the following specific steps:
(1) Removing impurities and stones from the fenugreek seeds, peeling, adding water for tempering, and crushing to 20-30 meshes to obtain fenugreek seed powder;
(2) Slicing fresh konjak, soaking in saturated lime water, filtering, pulverizing into colloidal substance by a refiner, adding water, shearing to obtain slurry, adding cellulase and alpha-amylase into the slurry, and performing enzymolysis to obtain a pre-enzymolysis product;
(3) Culturing Aureobasidium pullulans by secondary seeds to obtain seed liquid, and inoculating the seed liquid into a fermentation medium containing sucrose for culturing to obtain pullulan fermentation liquor;
(4) Finally, adding the fenugreek seed powder obtained in the step (1) and the pre-enzymolysis product obtained in the step (2) into the pullulan fermentation broth obtained in the step (3), then adding lipase, cellulase and neutral protease for enzymolysis to obtain an enzymolysis product, and performing post-treatment to obtain the thickener;
the specific method for post-treatment comprises the following steps: ultrafiltering the enzymolysis product with 10000Da ultrafilter membrane, concentrating to half of the original volume to obtain concentrated solution, adding 0.1-0.2 part of sodium chloride into 100 parts of concentrated solution, adding 10-15 parts of absolute ethyl alcohol, standing at 0-4 ℃ for 24-36 hours, centrifuging to obtain precipitate, slowly adding the precipitate into 250-300 parts of distilled water while stirring, fully stirring, centrifuging to remove insoluble matters;
in the step (1), the concrete method for adding water for tempering comprises the following steps: adding water with the weight being 0.02-0.03 times of that of the peeled fenugreek seeds, and carrying out ultrasonic oscillation for 5-7 minutes at the weight of 300-400W;
in the step (4), the mass ratio of the fenugreek seed powder to the pre-enzymolysis product to the pullulan fermentation liquor to the lipase to the cellulase to the neutral protease is 0.1-0.2: 80-90: 20-30: 0.08 to 0.1:0.1 to 0.12: 0.06-0.08.
2. The method according to claim 1, wherein in the step (2), the saturated lime water soaking time is 40 to 50 minutes.
3. The method according to claim 1, wherein in the step (2), the shearing process conditions are: shearing for 10-15 min at 1500-2500 r/min.
4. The preparation method according to claim 1, wherein in the step (2), the mass ratio of fresh konjak, saturated lime water, cellulase and alpha-amylase is 300-320: 650-680: 600-700: 2-3: 1.5 to 2.
5. The method according to claim 1, wherein in the step (2), the enzymatic hydrolysis is performed under the following conditions: pH=5-6, and enzymolysis at 45-50 deg.c for 2-3 hr.
6. The preparation method according to claim 1, wherein the specific method of step (3) is as follows: inoculating Aureobasidium pullulans into a seed culture medium, culturing for 22-25 hours at the temperature of 27-29 ℃ through a primary seed tank, inoculating into a secondary seed tank according to the inoculum size of 8-12% by volume, and culturing for 22-25 hours at the temperature of 27-29 ℃ to obtain seed liquid; and then inoculating the seed solution into a fermentation medium according to the inoculum size of 4-6% by volume, and culturing for 52-58 hours at the temperature of 27-29 ℃ to obtain the pullulan fermentation broth.
7. An environment-friendly electronic atomized liquid thickener obtained by the preparation method of any one of claims 1 to 6.
8. Use of the environment-friendly electronic atomized liquid thickener of claim 7 in electronic atomized liquid preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111243140.8A CN113974202B (en) | 2021-10-25 | 2021-10-25 | Environment-friendly electronic atomized liquid thickener and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111243140.8A CN113974202B (en) | 2021-10-25 | 2021-10-25 | Environment-friendly electronic atomized liquid thickener and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113974202A CN113974202A (en) | 2022-01-28 |
| CN113974202B true CN113974202B (en) | 2023-09-01 |
Family
ID=79741149
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111243140.8A Active CN113974202B (en) | 2021-10-25 | 2021-10-25 | Environment-friendly electronic atomized liquid thickener and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113974202B (en) |
Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1517547A (en) * | 1974-10-17 | 1978-07-12 | Japan Tobacco & Salt Public | Tobacco product containing a thermogelable beta-1,3-glucan-type polysaccharide |
| US5518902A (en) * | 1992-08-20 | 1996-05-21 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | High pullulan content product, and its preparation and uses |
| CN101400268A (en) * | 2006-01-11 | 2009-04-01 | 樱井诚也 | Fluid konjac material, method of producing the same and use thereof |
| CN101914597A (en) * | 2010-07-27 | 2010-12-15 | 中国农业科学院饲料研究所 | Method for preparing konjac mannan oligosaccharide |
| JP2011229440A (en) * | 2010-04-27 | 2011-11-17 | Nisshin Oillio Group Ltd | Thickener granule and method for producing the same |
| CN102816253A (en) * | 2012-09-11 | 2012-12-12 | 西北师范大学 | Method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling |
| WO2013053071A1 (en) * | 2011-10-10 | 2013-04-18 | Huang Daiyong | Production method for preparing high purity mannan oligosaccharide from enzymolysis of hemicellulose |
| CN103396498A (en) * | 2013-07-30 | 2013-11-20 | 无锡腾华粮油机械有限公司 | Fenugreek gum seed endosperm sheet separation process |
| CN103805650A (en) * | 2012-11-09 | 2014-05-21 | 山东福瑞达生物科技有限公司 | Fermentation production method for pigment-free pullulan |
| CN104432492A (en) * | 2014-11-03 | 2015-03-25 | 云南中烟工业有限责任公司 | Application of Pulullan or compound of Pulullan in cigarette |
| CN104448403A (en) * | 2014-12-05 | 2015-03-25 | 通辽梅花生物科技有限公司 | Pullulan polysaccharide gum and preparation process thereof |
| CN104725525A (en) * | 2015-04-15 | 2015-06-24 | 成都真颖精工机电设备有限公司 | Konjac glue preparation method |
| CN110150725A (en) * | 2019-05-29 | 2019-08-23 | 深圳市赛尔美电子科技有限公司 | Solid-state tobacco tar and its preparation method and application |
| CN111741687A (en) * | 2018-02-23 | 2020-10-02 | 株式会社东亚产业 | Method for producing non-tobacco plant composition, method for producing filler for electronic cigarette, and electronic cigarette cartridge using same |
| CN111836557A (en) * | 2018-04-06 | 2020-10-27 | 菲利普莫里斯生产公司 | Nicotine gel |
| CN112369597A (en) * | 2020-11-30 | 2021-02-19 | 中华全国供销合作总社南京野生植物综合利用研究所 | Composite thickening agent and preparation process thereof |
| CN112409506A (en) * | 2020-11-25 | 2021-02-26 | 山东福瑞达生物科技有限公司 | Method for preparing pullulan polysaccharides with different uniform molecular weights |
| CN113273716A (en) * | 2021-05-19 | 2021-08-20 | 深圳市真味生物科技有限公司 | Atomized liquid gel and preparation process thereof |
-
2021
- 2021-10-25 CN CN202111243140.8A patent/CN113974202B/en active Active
Patent Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1517547A (en) * | 1974-10-17 | 1978-07-12 | Japan Tobacco & Salt Public | Tobacco product containing a thermogelable beta-1,3-glucan-type polysaccharide |
| US5518902A (en) * | 1992-08-20 | 1996-05-21 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | High pullulan content product, and its preparation and uses |
| CN101400268A (en) * | 2006-01-11 | 2009-04-01 | 樱井诚也 | Fluid konjac material, method of producing the same and use thereof |
| JP2011229440A (en) * | 2010-04-27 | 2011-11-17 | Nisshin Oillio Group Ltd | Thickener granule and method for producing the same |
| CN101914597A (en) * | 2010-07-27 | 2010-12-15 | 中国农业科学院饲料研究所 | Method for preparing konjac mannan oligosaccharide |
| WO2013053071A1 (en) * | 2011-10-10 | 2013-04-18 | Huang Daiyong | Production method for preparing high purity mannan oligosaccharide from enzymolysis of hemicellulose |
| CN102816253A (en) * | 2012-09-11 | 2012-12-12 | 西北师范大学 | Method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling |
| CN103805650A (en) * | 2012-11-09 | 2014-05-21 | 山东福瑞达生物科技有限公司 | Fermentation production method for pigment-free pullulan |
| CN103396498A (en) * | 2013-07-30 | 2013-11-20 | 无锡腾华粮油机械有限公司 | Fenugreek gum seed endosperm sheet separation process |
| CN104432492A (en) * | 2014-11-03 | 2015-03-25 | 云南中烟工业有限责任公司 | Application of Pulullan or compound of Pulullan in cigarette |
| CN104448403A (en) * | 2014-12-05 | 2015-03-25 | 通辽梅花生物科技有限公司 | Pullulan polysaccharide gum and preparation process thereof |
| CN104725525A (en) * | 2015-04-15 | 2015-06-24 | 成都真颖精工机电设备有限公司 | Konjac glue preparation method |
| CN111741687A (en) * | 2018-02-23 | 2020-10-02 | 株式会社东亚产业 | Method for producing non-tobacco plant composition, method for producing filler for electronic cigarette, and electronic cigarette cartridge using same |
| CN111836557A (en) * | 2018-04-06 | 2020-10-27 | 菲利普莫里斯生产公司 | Nicotine gel |
| CN110150725A (en) * | 2019-05-29 | 2019-08-23 | 深圳市赛尔美电子科技有限公司 | Solid-state tobacco tar and its preparation method and application |
| CN112409506A (en) * | 2020-11-25 | 2021-02-26 | 山东福瑞达生物科技有限公司 | Method for preparing pullulan polysaccharides with different uniform molecular weights |
| CN112369597A (en) * | 2020-11-30 | 2021-02-19 | 中华全国供销合作总社南京野生植物综合利用研究所 | Composite thickening agent and preparation process thereof |
| CN113273716A (en) * | 2021-05-19 | 2021-08-20 | 深圳市真味生物科技有限公司 | Atomized liquid gel and preparation process thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113974202A (en) | 2022-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102839075B (en) | Production process of fig wine | |
| CN110786538B (en) | Nicotine formulation, preparation method thereof and electronic cigarette oil | |
| CN106136303A (en) | A kind of preparation method and applications of Fructus Gardeniae cigarette extract | |
| CN113667360A (en) | Humic acid type jujube fruit cracking prevention film agent and preparation method and application thereof | |
| CN104745304A (en) | Maillard reaction product prepared by taking rose, pomegranate and fermented glutinous rice as raw materials and application thereof in cigarette | |
| CN113974202B (en) | Environment-friendly electronic atomized liquid thickener and preparation method thereof | |
| CN114213555B (en) | Okra polysaccharide extract and preparation method thereof | |
| CN114381235B (en) | Efficient environment-friendly biological composite dust suppressant and preparation method thereof | |
| Liu et al. | Characterization of polysaccharides extracted from a cultivated brown alga Costaria costata during the harvest period | |
| CN105199939A (en) | Preparation method of vinegar having low trimethylamine acetate content and rich konjac high-F-ratio oligopeptide | |
| CN105029687A (en) | Preparing method for cigarette addition agent rich in barley polysaccharide | |
| CN108504362A (en) | A kind of alkaline land modifying agent and preparation method thereof | |
| CN114989984B (en) | Method for preparing seaweed extract by enzymolysis | |
| CN103222679A (en) | Method for preparing tobacco fragment extractives by high-pressure-puffing-flocculation and application of extractives | |
| CN103734905B (en) | A kind of preparation method of deproteinized tobacco extract and application | |
| CN105747265A (en) | Fresh-scent type tobacco sheet tobacco shreds and preparing method thereof | |
| CN110742302B (en) | Cigarette capable of reducing free radical content in smoke and preparation method thereof | |
| CN108441226A (en) | A kind of soil conditioner and preparation method thereof for salt-soda soil | |
| CN105768196A (en) | Sheet tobacco shred containing water chestnut hull and preparation method thereof | |
| CN105768183A (en) | Tobacco flakes capable of soothing the nerves and aiding sleep and production method thereof | |
| CN105768188A (en) | Sheet tobacco shred containing pine bark and preparation method thereof | |
| CN104013096A (en) | Preparation method of papermaking-process reconstituted tobaccos capable of reducing content of CO in mainstream smoke | |
| CN105886324B (en) | A kind of Selenium-enriched fermentation method of indole-3-carbinol and indole -3-acetonitrile in raising fruit vinegar | |
| CN114794526B (en) | Method for improving combustibility of cigar tobacco leaves | |
| CN113615862B (en) | Aromatic plant electronic atomized liquid and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |