CN113969239A - Full-automatic cell culture system - Google Patents

Full-automatic cell culture system Download PDF

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Publication number
CN113969239A
CN113969239A CN202111314966.9A CN202111314966A CN113969239A CN 113969239 A CN113969239 A CN 113969239A CN 202111314966 A CN202111314966 A CN 202111314966A CN 113969239 A CN113969239 A CN 113969239A
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China
Prior art keywords
cell
culture
cell culture
fixed frame
culture chamber
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CN202111314966.9A
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Chinese (zh)
Inventor
杨罕闻
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Lanlian Hangzhou Biotechnology Co ltd
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Lanlian Hangzhou Biotechnology Co ltd
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Priority to CN202111314966.9A priority Critical patent/CN113969239A/en
Publication of CN113969239A publication Critical patent/CN113969239A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a full-automatic cell culture system, which relates to the field of production machinery, and comprises: the cell culture device comprises a culture chamber, a fixed frame arranged in the culture chamber, a cell culture fiber membrane arranged on the fixed frame, a cell filter screen arranged on the fixed frame and positioned beside the cell culture fiber membrane, a cell input assembly connected with the culture chamber and arranged on the cell culture fiber membrane, a culture solution automatic input assembly connected with the culture chamber, a blowing assembly arranged in the culture chamber and positioned on the cell culture fiber membrane, a centrifugal rotating assembly arranged in the culture chamber and positioned below the fixed frame, an oxygen bottle connected with the centrifugal rotating assembly, and a cell suspension suction assembly arranged above the fixed frame; the invention can automatically complete cell input, culture solution input and cell suspension collection; the whole process is automatic, the operation is accurate, the manpower is reduced, the industrial production is realized, the culture efficiency is improved, and the cell yield and the cell purity are improved.

Description

Full-automatic cell culture system
Technical Field
The invention relates to the field of production machinery, in particular to a full-automatic cell culture system.
Background
The cell culture on the market generally comprises the following steps: collecting cells, and adding reagents such as buffer solution and the like; step two, changing the test tube into a new test tube, and culturing the test tube by using a culture solution; and step three, replacing the culture bottle and adding a culture reagent, suspending the cells, then centrifuging, and then storing the cells. Countless instruments are needed in the whole process, the whole process is manual, misoperation is easy to occur, and the cell yield and purity are reduced; the market needs a cell culture system which can realize full-automatic cell culture, reduce manpower, has accurate operation and improves the cell yield and purity, and the invention solves the problems.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide a full-automatic cell culture system which can automatically complete cell input, culture solution input and cell suspension collection; the whole process is automatic, the operation is accurate, the manpower is reduced, the industrial production is realized, the culture efficiency is improved, and the cell yield and the cell purity are improved.
In order to achieve the above object, the present invention adopts the following technical solutions:
a fully automated cell culture system, comprising: the cell culture device comprises a culture chamber, a fixed frame arranged in the culture chamber, a cell culture fiber membrane arranged on the fixed frame, a cell filter screen arranged on the fixed frame and positioned beside the cell culture fiber membrane, a cell input assembly connected with the culture chamber and arranged on the cell culture fiber membrane, an automatic culture solution input assembly connected with the culture chamber, a blowing assembly arranged in the culture chamber and positioned on the cell culture fiber membrane, a centrifugal rotating assembly arranged in the culture chamber and positioned under the fixed frame, an oxygen bottle connected with the centrifugal rotating assembly, and a cell suspension liquid suction assembly arranged above the fixed frame.
In the fully automatic cell culture system, the cell filter screen is a cell filter screen with a size of 40-100 μm.
In the fully automatic cell culture system, the cell input assembly comprises: a cell insert, a connection pipe connected to the cell insert, and a nozzle connected to the connection pipe.
In the fully automatic cell culture system, the automatic culture solution input assembly comprises: a reagent bottle for storing reagent, a liquid conveying pipe connected between the reagent bottle and the culture chamber, and a precise quantitative liquid valve arranged on the liquid conveying pipe.
The full-automatic cell culture system comprises a blowing assembly and a control assembly, wherein the blowing assembly comprises: the hair-dryer connects in the scalable blowing pipe of hair-dryer, connects in the blowing head of scalable blowing pipe, is fixed in the automatic telescopic link of scalable blowing pipe.
In the fully automatic cell culture system, the rotating shaft is arranged between the blowing head and the telescopic air outlet pipe.
In the fully automatic cell culture system, the telescopic air outlet pipe is a corrugated telescopic air outlet pipe.
The fully automatic cell culture system comprises the following components: the rotary motor is arranged under the culture chamber, the connecting shaft is connected with the rotary motor and penetrates through the culture chamber, the hollow rotary drum is connected with the connecting shaft, and the oxygen tube is connected between the hollow rotary drum and the oxygen bottle.
The fully automatic cell culture system comprises the following hollow rotary drum: the inner wall of the rotary drum, the outer wall of the rotary drum and a hollow layer which is formed between the inner wall of the rotary drum and the outer wall of the rotary drum and connected with the oxygen pipe.
In the fully automatic cell culture system, the inner wall of the rotary drum is provided with the through hole.
The invention has the advantages that:
the invention can automatically complete cell input, culture solution input and cell suspension collection; the whole process is automatic, the operation is accurate, the labor is reduced, and the industrial production is realized;
the invention has no interference, does not need to manually clean the bottle, has no pollution risk, improves the culture efficiency and simultaneously improves the cell yield and purity;
the cell culture device is provided with the blowing assembly, so that cultured cells can be blown up from the cell culture fiber membrane to avoid being adhered to the fiber membrane, cell suspension is gathered above the fixed frame through centrifugation and isolation of the cell filter screen and is sucked by the cell suspension sucking assembly, collection is completed, the whole process is free of interference, the efficiency is high, and the culture effect is good;
the oxygen bottle is connected with the hollow rotary drum, and oxygen leaks out from the through hole on the inner wall of the rotary drum, so that the oxygen can be intensively supplied to the cell culture fiber membrane.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
figure 2 is a schematic structural view of an embodiment of the fixing frame of the invention;
FIG. 3 is a schematic structural view of an embodiment of an idle drum of the present invention;
the meaning of the reference symbols in the figures:
1 culture chamber, 2 fixed frames, 3 cell culture fibrous membranes, 4 cell filter screens, 5 oxygen bottles, 6 injectors, 7 connecting pipes, 8 booster pumps, 9 spray heads, 10 reagent bottles, 11 infusion tubes, 12 precise quantitative liquid valves, 13 blowers, 14 telescopic blowpipes, 15 blowing heads, 16 automatic telescopic rods, 17 rotating shafts, 18 rotating motors, 19 connecting shafts, 20 hollow rotary drums, 201 rotary drum inner walls, 202 rotary drum outer walls, 203 hollow layers, 204 through holes, 21 oxygen tubes, 22 liquid suction pumps and 23 liquid suction pipes.
Detailed Description
The invention is described in detail below with reference to the figures and the embodiments.
As shown in fig. 1 and 2, a fully automatic cell culture system includes: the culture room 1, set up the fixed frame 2 in the culture room 1, set up the cell culture fibrous membrane 3 on the fixed frame 2, set up on the fixed frame 2 and be located the cell culture fibrous membrane 3 by the cell filter screen 4, connect culture room 1 and set up the cell input subassembly on the cell culture fibrous membrane 3, connect the automatic input subassembly of culture solution in culture room 1, set up in culture room 1 and be located the subassembly of blowing on the cell culture fibrous membrane 3, set up in culture room 1 and be located the centrifugal rotating assembly under the fixed frame 2, connect in the oxygen cylinder 5 of centrifugal rotating assembly, set up in the cell suspension of fixed frame 2 top absorbs the subassembly.
Preferably, the cell strainer 4 is a cell strainer 4 of 40 to 100 μm; the cell strainer 4 is selected according to the type of cells, and is not limited. The cell input assembly comprises: a cell insert, a connecting tube 7 connected to the cell insert, and a nozzle 9 connected to the connecting tube 7. Preferably, the nozzle 9 has a flared opening to limit the extent of the cell spray. The cell input device is preferably a syringe 6, and is not limited as long as it can input cells into the culture chamber 1. In order to disperse the cells, a booster pump 8 is arranged on the connecting pipe 7, and the boosting range is 5-10 pa.
The automatic culture solution input assembly comprises: a reagent bottle 10 for storing reagent, a perfusion tube 11 connected between the reagent bottle 10 and the culture chamber 1, and a precise quantitative liquid valve 12 arranged on the perfusion tube 11; the flux of the precise quantitative liquid valve 12 is set according to the cell culture requirement, and the whole process of aseptic liquid feeding is realized.
The subassembly of blowing includes: a blower 13, a telescopic blowing pipe 14 connected to the blower 13, a blowing head 15 connected to the telescopic blowing pipe 14, and an automatic telescopic rod 16 fixed to the telescopic blowing pipe 14; in order to blow up cells in multiple directions conveniently, a rotating shaft 17 is arranged between the blowing head 15 and the telescopic air outlet pipe, so that the direction of the blowing head 15 can be adjusted conveniently, and as a preferred mode, the automatic telescopic rod 16 is an electric stainless steel telescopic rod. Preferably, the cell culture fibrous membrane 3 is a cell culture fibrous membrane 3 prepared by an electrostatic spinning process; the cell culture fibrous membrane 3 made of the material can not stick to cells, the cells can be easily blown up by the blower 13, the blown-up cells are isolated on the upper layer by the cell culture fibrous membrane 3 and the cell filter screen 4, and the cells are sucked and collected by the cell suspension liquid sucking component after being centrifuged. The cell suspension suction assembly comprises: a pipette 23 connected to the culture chamber 1, and a pipette pump 22 provided in the pipette 23; the pipette 23 is connected to a collection vessel for frozen storage.
As an optimization, the telescopic air outlet pipe is a corrugated telescopic air outlet pipe, and a corrugated aluminum pipe is selected as the corrugated telescopic air outlet pipe.
The centrifugal rotation assembly includes: a rotary motor 18 disposed under the culture chamber 1, a connecting shaft 19 connected to the rotary motor 18 and penetrating the culture chamber 1, a hollow rotary drum 20 connected to the connecting shaft 19, and an oxygen tube 21 connected between the hollow rotary drum 20 and the oxygen bottle 5; sealing gaskets and a rotary shaft 17 bearing are arranged among the connecting shaft 19, the hollow rotary drum 20 and the culture chamber 1. The rotating motor 18 drives the connecting shaft 19 to drive the hollow rotary drum 20 to rotate, so as to drive the liquid in the culture chamber 1 to rotate, and the blown cells and the culture solution are uniformly mixed. As shown in fig. 3, the hollow drum 20 includes: a drum inner wall 201, a drum outer wall 202, a hollow layer 203 formed between the drum inner wall 201 and the drum outer wall 202 and connected to the oxygen pipe 21; the drum inner wall 201 is provided with a through hole 204. Oxygen enters the oxygen tube 21 from the oxygen bottle 5 and then enters the culture chamber 1 from the inner wall 201 of the rotary drum through the through hole 204, so that oxygen can be supplied to the cell culture fibrous membrane 3 in a centralized manner, and a replacement gas device can be arranged in the culture chamber 1 as required to adjust the ratio of carbon dioxide to oxygen in the culture chamber 1.
The using process of the invention is as follows: preparing cell suspension and culture solution, injecting the cell suspension into an injector 6, injecting the culture solution into a reagent bottle 10, moving a connecting pipe 7 uniformly, and putting the cell suspension and culture solution on the cell culture fibrous membrane 3 by the injector 6. Opening a precise quantitative liquid valve 12 according to the requirement, inputting the culture reagent into the culture chamber 1, adjusting the type and the dosage of the culture reagent according to the requirement in the period, adjusting the input amount of oxygen, blowing the cells from the cell culture fiber membrane 3 by using a blower 13 after the culture is finished, starting a rotating motor 18, driving a hollow rotary drum 20, centrifugally rotating the cells and the culture solution to ensure that the cells are uniformly distributed in the culture solution on the upper layer of a fixed frame 2, collecting the cell suspension by using a liquid suction pump 22 and a liquid suction pipe 23, and freezing and storing.
The invention can automatically complete cell input, culture solution input and cell suspension collection; the whole process is automatic, the operation is accurate, the labor is reduced, and the industrial production is realized; the invention has no interference, does not need to manually clean the bottle, has no pollution risk, improves the culture efficiency and simultaneously improves the cell yield and purity.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.

Claims (10)

1. A fully automated cell culture system, comprising: the cell culture device comprises a culture chamber, a fixed frame arranged in the culture chamber, a cell culture fiber membrane arranged on the fixed frame, a cell filter screen arranged on the fixed frame and positioned beside the cell culture fiber membrane, a cell input assembly connected with the culture chamber and arranged on the cell culture fiber membrane, an automatic culture solution input assembly connected with the culture chamber, a blowing assembly arranged in the culture chamber and positioned on the cell culture fiber membrane, a centrifugal rotating assembly arranged in the culture chamber and positioned under the fixed frame, an oxygen bottle connected with the centrifugal rotating assembly, and a cell suspension liquid suction assembly arranged above the fixed frame.
2. The fully automated cell culture system according to claim 1, wherein the cell filter is a 40-100 μm cell filter.
3. The fully automated cell culture system of claim 1, wherein the cell input assembly comprises: a cell insert, a connection pipe connected to the cell insert, and a nozzle connected to the connection pipe.
4. The fully automated cell culture system according to claim 1, wherein the automatic culture solution input assembly comprises: a reagent bottle for storing reagent, a liquid conveying pipe connected between the reagent bottle and the culture chamber, and a precise quantitative liquid valve arranged on the liquid conveying pipe.
5. The fully automated cell culture system of claim 1, wherein the blower assembly comprises: the hair-dryer connects in the scalable blowing pipe of hair-dryer, connects in the blowing head of scalable blowing pipe, is fixed in the automatic telescopic link of scalable blowing pipe.
6. The fully automatic cell culture system according to claim 5, wherein a rotating shaft is arranged between the blowing head and the retractable air outlet pipe.
7. The fully automatic cell culture system according to claim 5, wherein the retractable air outlet pipe is a corrugated retractable air outlet pipe.
8. The fully automated cell culture system of claim 1, wherein the centrifugal rotation assembly comprises: the rotary motor is arranged under the culture chamber, the connecting shaft is connected with the rotary motor and penetrates through the culture chamber, the hollow rotary drum is connected with the connecting shaft, and the oxygen tube is connected between the hollow rotary drum and the oxygen bottle.
9. The fully automated cell culture system of claim 8, wherein the hollow rotating cylinder comprises: the inner wall of the rotary drum, the outer wall of the rotary drum and a hollow layer which is formed between the inner wall of the rotary drum and the outer wall of the rotary drum and connected with the oxygen pipe.
10. The fully automated cell culture system according to claim 9, wherein the inner wall of the bowl is provided with through holes.
CN202111314966.9A 2021-11-08 2021-11-08 Full-automatic cell culture system Pending CN113969239A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111314966.9A CN113969239A (en) 2021-11-08 2021-11-08 Full-automatic cell culture system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111314966.9A CN113969239A (en) 2021-11-08 2021-11-08 Full-automatic cell culture system

Publications (1)

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CN113969239A true CN113969239A (en) 2022-01-25

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CN202111314966.9A Pending CN113969239A (en) 2021-11-08 2021-11-08 Full-automatic cell culture system

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CN (1) CN113969239A (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201933085U (en) * 2010-11-11 2011-08-17 天津开发区合普工贸有限公司 Cell-exposure oxygen-deficient environment experimental device
WO2014034146A1 (en) * 2012-09-03 2014-03-06 三菱レイヨン株式会社 Cell culture module
CN108395982A (en) * 2018-05-30 2018-08-14 厦门市科环海洋生物科技有限公司 A kind of bacterium colony spraying device
CN207775256U (en) * 2017-11-22 2018-08-28 刘卓一 A kind of cell culture apparatus for Senior Biology scientific experiment

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201933085U (en) * 2010-11-11 2011-08-17 天津开发区合普工贸有限公司 Cell-exposure oxygen-deficient environment experimental device
WO2014034146A1 (en) * 2012-09-03 2014-03-06 三菱レイヨン株式会社 Cell culture module
CN207775256U (en) * 2017-11-22 2018-08-28 刘卓一 A kind of cell culture apparatus for Senior Biology scientific experiment
CN108395982A (en) * 2018-05-30 2018-08-14 厦门市科环海洋生物科技有限公司 A kind of bacterium colony spraying device

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