CN113968024B - Accurate temperature control type biological 3D printing system - Google Patents

Abstract

The invention relates to an accurate temperature control type biological 3D printing system which comprises a temperature control device, a temperature control printing head, a storage bin and a temperature control feeding pipe, wherein the temperature control printing head is movably arranged on the upper side of the temperature control device, the storage bin is arranged in a water bath temperature control box and is connected with the temperature control printing head through the temperature control feeding pipe, the temperature control device comprises a cover body, a box body and a base which are sequentially arranged from top to bottom, a first refrigeration assembly is arranged in the cover body, a second refrigeration assembly is arranged in the base, an infrared observation window is arranged on the side wall of the box body, a temperature measurement assembly corresponding to the infrared observation window is arranged in the box body, the box body comprises a plurality of connecting shells which are embedded and overlapped, the temperature measurement assembly comprises a temperature measurement base and a plurality of temperature measurement modules, and the temperature measurement modules are sequentially embedded and overlapped. The temperature control device has uniform internal temperature, ensures accurate temperature control, can adjust the height according to requirements, and is provided with heat insulation structures on the outer sides of the printing nozzle and the conveying pipe.

Description

A precise temperature-controlled biological 3D printing system

technical field

The invention relates to the technical field of biological 3D printing, in particular to a precise temperature-controlled biological 3D printing system.

Background technique

With the rapid development of human society, biomedical engineering has gradually attracted people's attention, especially the rapid development of bio-3D printing technology. At present, bio-3D printing technology mainly uses extrusion and inkjet printing. Extrusion printing is limited by the structure of the printing equipment and materials used. The materials are prone to problems such as broken filaments and cell damage during the extrusion process. The bio-inks used in the 3D bioprinter are mostly temperature-sensitive materials such as Gelatin and GelMa, because they are in a liquid state above the gel temperature and in a gel state below the gel temperature, while the internal temperature of the nozzle is above the gel temperature, which makes The bio-ink inside is in a liquid state, so the nozzle of the inkjet bio-3D printer uses piezoelectric or thermal bubble driving methods to generate tiny droplets at the nozzle mouth, and the droplets are quickly ejected and pass through the temperature control device In the set gas temperature field, after the convective heat exchange between the droplet and the gas temperature is completed, its state becomes a gel state and is bonded to the bottom plate of the temperature control device, but in the process of inkjet bio-printing, due to the Some of the bioinks have the characteristics of temperature sensitivity and the activity of cells is greatly affected by temperature, so it is particularly important to control the temperature during the printing process.

In addition, the temperature control device of the biological 3D printer in the prior art is prone to temperature gradients in the air inside due to the limitation of the layout of the local refrigeration device, thereby causing uneven temperature distribution inside the device. The analysis of the gas temperature field is generally based on the temperature of the heat source of the temperature control device to calculate the internal gas temperature field, which cannot analyze the actual temperature of the internal gas, resulting in the inaccurate control of the gas temperature field inside the temperature control device of the biological 3D printer. affect the printing effect.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a precise temperature-controlled biological 3D printing system, in which the temperature inside the temperature control device is uniform, and the temperature field distribution inside the box is accurately reflected by using the temperature measuring component and the infrared observation device, so as to ensure accurate temperature control , to ensure the printing effect, and the height of the box and temperature measurement components of the temperature control device can be adjusted according to needs, and the application range is wide.

The purpose of this invention is to realize through the following technical solutions:

An accurate temperature-controlled biological 3D printing system, comprising a temperature control device, a temperature control print head, a silo and a temperature control feed pipe, wherein the temperature control print head is movably arranged on the upper side of the temperature control device, and the silo is placed in a A water bath temperature control box is connected to the temperature control print head through a temperature control feeding pipe. The temperature control device includes a cover body, a box body and a base arranged in sequence from top to bottom, and the cover body is provided with a first In the refrigeration component, a second refrigeration component is arranged in the base, an infrared observation window is arranged on the side wall of the box body, and a temperature measurement component is arranged inside the box body corresponding to the infrared observation window.

The box body includes a plurality of connection shells that are sequentially fitted and stacked along the height direction, the temperature measurement assembly) includes a temperature measurement base and a plurality of temperature measurement modules, and each temperature measurement module is sequentially fitted and stacked along the height direction.

The upper end of the connection shell is provided with a connection positioning groove, and the lower end is provided with a connection positioning protrusion. The sleeves are stacked in sequence with the connection shells of each layer, and the cavities inside the insulation sleeves are connected in sequence after the stacking; the temperature measurement module includes a first bracket and a first heat-conducting member, and the upper side of the first bracket is provided with There is a first connecting protrusion), a limiting groove is provided on the lower side, the temperature measuring base includes a second bracket and a second heat conducting member, and the upper side of the second bracket is provided with a second connecting protrusion.

Both ends of the first heat-conducting member are respectively mounted on the first bracket through first heat insulation blocks, and the upper and lower sides of the first support are provided with heat-insulating grooves, and both ends of the second heat-conducting member are They are respectively installed on the second brackets through the second heat insulation blocks, and the lower ends of the legs of the second brackets are provided with heat insulation pads.

The lower side of the cover body is provided with a lower cover plate, and the first refrigeration component is fixedly mounted on the lower cover plate through a first fixed pressure plate, and the first refrigeration component includes a first semiconductor refrigeration element and a first cooling element. The first semiconductor refrigeration component is embedded between the first cooling component and the lower cover plate; the base is provided with a mounting plate, and the second refrigeration component is fixedly mounted on the On the mounting plate, the second refrigeration assembly includes a second semiconductor refrigeration element and a second cooling element, and the second semiconductor refrigeration element is embedded between the second cooling element and the mounting plate.

The cover body includes an upper insulation cover and a lower cover, wherein the upper insulation cover is buckled on the lower cover, the first refrigeration component is arranged in the upper insulation cover and mounted on the lower cover, and the upper insulation cover The middle part and the middle part of the lower cover plate are provided with printing through holes, one side of the upper heat preservation cover is provided with a connecting through hole for the first refrigeration component joint to pass through, and the lower side of the lower cover plate is provided with cover plate protrusions department.

The base includes a thermal insulation shell and a mounting plate, wherein the thermal insulation shell includes a thermal insulation top plate with an installation opening in the middle, the mounting plate is installed on the lower side of the thermal insulation top plate, and the second refrigeration component is arranged in the thermal insulation shell and installed on the installation On one end of the thermal insulation shell, an opening through which the joint of the second refrigeration assembly passes is provided, and the upper side of the mounting plate is provided with an installation opening through which an installation positioning groove passes through the middle of the thermal insulation top plate.

The temperature control print head includes a print head, a temperature control jacket and a print head mounting plate, wherein the end of the print head is fixedly mounted on the print head mounting plate, the temperature control jacket is sleeved on the print head, and the temperature control A temperature control cavity is arranged inside the jacket, and a water inlet and a water outlet are arranged on the temperature control jacket to communicate with the temperature control cavity, and a touch sensor insertion port and a heating rod insertion port are arranged on one side of the temperature control jacket .

The temperature control feeding pipe includes an external temperature control pipe and a material conveying pipe arranged in the external temperature control pipe. A water bath flow channel is formed between the outer wall of the material conveying pipe and the inner wall of the external temperature control pipe. The material pipe is communicated with the silo, and the water bath flow channel is communicated with the water bath temperature control box.

Both ends of the temperature control feeding pipe are provided with connecting joints, one side of the connecting joint is provided with a first connecting part to connect with the external temperature control pipe, and the other side is provided with a second connecting part for sealing connection with a gland. , the feeding pipe passes through the connecting joint, the connecting joint of the input end of the temperature-controlled feeding pipe is connected with a water inlet pipe, the connecting joint of the output end of the temperature-controlled feeding pipe is connected with a water outlet pipe, and the water inlet pipe and the outlet pipe are connected with each other. The water pipes are all communicated with the water bath temperature control box.

The advantages and positive effects of the present invention are:

1. The present invention uses the temperature control device cover and the refrigeration components in the base to simultaneously cool the inside of the box, which can greatly reduce the temperature gradient inside the entire device, so that the low temperature inside the entire temperature control device tends to be uniform, which is more conducive to spraying. Ink-type biological 3D printing improves the printing effect. In addition, the present invention can more accurately reflect the temperature distribution inside the box by using the temperature measuring component and the infrared observation device, so that the actual temperature inside the box can be accurately controlled.

2. The box body of the temperature control device of the present invention and the temperature measuring components inside it include a plurality of modules that are sequentially fitted and stacked in the height direction, which can be adjusted according to printing needs and have a wider application range.

3. In the present invention, a temperature control jacket is arranged outside the printing nozzle to monitor and adjust the internal temperature of the printing nozzle, and an external temperature control pipe is arranged outside the material conveying pipe, and a water bath flow channel between the material conveying pipe and the external temperature control pipe is arranged. It is connected with the water bath temperature control box, so as to realize the thermal insulation effect during the transfer of biological materials.

Description of drawings

Fig. 1 is the structural representation of the present invention,

Fig. 2 is a three-dimensional schematic diagram of the temperature control device in Fig. 1,

Fig. 3 is a sectional view of the temperature control device in Fig. 2,

Fig. 4 is a three-dimensional schematic diagram of the upper thermal insulation cover in Fig. 3,

FIG. 5 is a schematic diagram of the installation of the first cooling element in FIG. 3,

FIG. 6 is a schematic diagram of the bottom side structure of the lower cover plate in FIG. 5 ,

FIG. 7 is a three-dimensional schematic diagram of the box in FIG. 2 ,

Fig. 8 is the three-dimensional schematic diagram of the thermal insulation jacket in Fig. 7,

FIG. 9 is a schematic diagram of the temperature measurement assembly and the printing structure in the box in FIG. 3 ,

Fig. 10 is a three-dimensional schematic diagram of the temperature measuring assembly in Fig. 9,

Fig. 11 is a three-dimensional schematic diagram of the temperature measurement module in Fig. 10,

Fig. 12 is a three-dimensional schematic diagram of the temperature measuring base in Fig. 10,

Figure 13 is a schematic diagram of the connection between the temperature measurement base in Figure 10 and an adjacent temperature measurement module,

Fig. 14 is a three-dimensional schematic view of the base in Fig. 2,

Fig. 15 is a schematic structural diagram of the base in Fig. 14 when the thermal insulation side plate is removed,

Fig. 16 is a schematic diagram of another angle structure of the base in Fig. 15,

FIG. 17 is a cross-sectional view of the temperature-controlled print head in FIG. 1 ,

Figure 18 is a left side view of the temperature-controlled print head in Figure 17,

Figure 19 is a schematic diagram of the internal structure of the temperature control feeding pipe in Figure 1,

Fig. 20 is a cross-sectional view of the temperature-controlled feeding pipe in Fig. 19,

Fig. 21 is a schematic diagram of the structure of the feeding end of the temperature-controlled feeding pipe in Fig. 19,

FIG. 22 is a schematic diagram of the structure of the discharge end of the temperature-controlled feeding tube in FIG. 19 .

Among them, 1 is the cover, 11 is the upper heat preservation cover, 111 is the printing through hole, 12 is the first cooling member, 13 is the first bolt, 14 is the first fixed pressing plate, 15 is the first heat preservation bottom plate, and 16 is the second Insulation bottom plate, 17 is the first semiconductor refrigeration element, 18 is the lower cover plate, 19 is the convex part of the cover plate, 2 is the box body, 21 is the insulation cover, 211 is the cavity, 212 is the notch, and 22 is the connection shell , 23 is the observation window, 24 is the connection positioning groove, 25 is the infrared observation window, 3 is the base, 31 is the thermal insulation side plate, 32 is the thermal insulation top plate, 33 is the third bolt, 34 is the installation positioning groove, 35 is the installation plate, 36 is the second cooling member, 37 is the second bolt, 38 is the second fixed platen, 39 is the second semiconductor refrigeration member, 4 is the temperature control print head, 401 is the print nozzle, 402 is the temperature control jacket, 403 is Water inlet, 404 is the water outlet, 405 is the print head mounting plate, 406 is the tactile sensor insertion port, 407 is the heating rod insertion port, 5 is the water bath temperature control box, 6 is the silo, 7 is the temperature control feeding pipe, 701 is the External temperature control pipe, 702 is the feeding pipe, 703 is the water bath channel, 704 is the connecting joint, 7041 is the first connecting part, 7042 is the second connecting part, 705 is the water inlet pipe, 706 is the gland, and 707 is the water outlet pipe , 8 is the workbench, 9 is the temperature measuring component, 91 is the temperature measuring module, 911 is the first bracket, 9111 is the first connecting protrusion, 9112 is the limit groove, 9113 is the insulation groove, and 912 is the first spacer Heat block, 913 is the first heat conducting member, 92 is the temperature measuring base, 921 is the second bracket, 9211 is the second connecting protrusion, 922 is the second heat insulating block, 923 is the second heat conducting member, 924 is the heat insulating pad .

Detailed ways

The present invention will be described in further detail below in conjunction with the accompanying drawings.

As shown in Figures 1-22, the present invention includes a temperature control device, a temperature control print head 4, a silo 6 and a temperature control feed pipe 7, wherein the temperature control print head 4 is movably arranged on the upper side of the temperature control device, and the silo 6 is set in a water bath temperature control box 5 and connected to the temperature control print head 4 through a temperature control feeding pipe 7, as shown in Figures 2 to 3, the temperature control device includes a cover body arranged in order from top to bottom 1. Box 2 and base 3. As shown in Figures 3 to 5, the cover body 1 is provided with a first refrigeration component. As shown in Figures 14 to 16, the base 3 is provided with a second refrigeration component , the first refrigeration assembly and the second refrigeration assembly simultaneously refrigerate the air near the cover 1 and the base 3 inside the temperature control device, which can greatly reduce the temperature gradient inside the entire device, so that the temperature inside the entire device tends to be uniform, It is more conducive to inkjet biological 3D printing and improves the printing effect. As shown in Figures 9 to 13, an infrared observation window 25 is provided on the side wall of the box body 2, and a temperature measurement component 9 is arranged inside the box body 2 corresponding to The infrared observation window 25 and the temperature measurement assembly 9 include a plurality of temperature measurement modules 91, and the temperature measurement modules 91 have a good heat conduction effect and can quickly conduct heat transfer with the air in the vicinity, so that the modules and the adjacent air can be quickly transferred. By observing the temperature of each module, the infrared observation device can accurately reflect the temperature distribution inside the box 2, and then monitor the printing and forming temperature of the biological material. The present invention can more accurately measure the actual temperature inside the box 2. The temperature is controlled to enhance the printing effect. When the inside of the box body 2 reaches the required temperature, the temperature-controlled print head 4 containing the biological material enters the inside of the box body 2. Under the influence of the lower temperature inside the box body 2, the biological material changes from liquid to gel state and drops to the It is arranged on the printing structure in the temperature control device, and the temperature control print head 4 moves according to the path set by the system to complete the printing and forming. The infrared observation device and the printing structure in the temperature control device are well known in the art.

As shown in FIG. 3 and FIG. 7 , the box body 2 includes a plurality of connecting shells 22 , and the connecting shells 22 are provided with connecting and positioning grooves 24 at the upper end and connecting positioning protrusions at the lower end. The connection positioning grooves 24 at the upper end of the body 22 cooperate with the connection positioning protrusions at the lower end of the adjacent connection shells 22 on the upper side, so as to realize the stacking of the connection shells 22 in sequence along the height direction, so that the box body 2 can be adjusted as required. The height is matched with the printing height of biological materials, and the application range is wider. In this embodiment, the connection housing 22 is made of aluminum material.

As shown in FIGS. 7 to 8 , the outer side of the connection housing 22 can be fitted with a thermal insulation jacket 21 as required, and the thermal insulation jacket 21 matches the shape of the connection housing 22 to prevent the temperature inside the connection module from being caused by external air. Affect the printing effect. As shown in FIG. 8 , in this embodiment, cavities 211 are provided in both side walls of the heat preservation sleeve 21 , and a notch 212 is provided on the cavity wall inside the cavity 211 , as shown in FIG. 2 , Each layer of thermal insulation jackets 21 can be stacked in sequence with each layer of connecting shells 22, and after stacking, the cavities 211 inside each thermal insulation jacket 21 are connected in sequence, and the thermal insulation effect can be achieved after injecting water of appropriate temperature. The shell 22 is provided with a heat preservation water inlet to communicate with its inner cavity 211 , and another connection shell 22 is provided with a heat preservation water outlet to communicate with its inner cavity 211 . In addition, as shown in FIG. 8 , one side of the heat insulating sleeve 21 is provided with an opening, so that the heat insulating sleeve 21 can have a slight opening width so as to be easily fitted on the corresponding connection housing 22 .

As shown in FIG. 9 , one side of the box body 2 is provided with an infrared observation window 25 , and the other side is provided with an observation window 23 . In this embodiment, the infrared observation window 25 is made of germanium lens, which can pass infrared rays, so that it is convenient to use an external infrared observation device to observe the temperature distribution of the temperature measurement module 31. The observation window 23 is made of transparent glass, so that the During the printing process, the operator can observe the state of the biological material on the printed structure in real time, and can also use a high-speed camera to capture the gel process of the bio-ink droplets, which can be used to improve the printing parameters of the printer.

As shown in FIGS. 10-13 , the temperature measurement assembly 9 includes a temperature measurement base 92 and a plurality of temperature measurement modules 91 , wherein the temperature measurement module 91 includes a first bracket 911 and a first heat conducting member 913 , and the first A bracket 911 is provided with a first connecting protrusion 9111 on the upper side, and a limiting groove 9112 on the lower side. The temperature measurement base 92 includes a second bracket 921 and a second heat conducting member 923, and the second bracket 921 has a A second connecting protrusion 9211 is provided. In the present invention, the height of the temperature measuring assembly 9 can be adjusted according to actual needs, so as to match the height adjustment of the box body 2, wherein as shown in FIG. The limit grooves 9112 on the lower side of the first bracket 911 adjacent to the temperature measurement module 91 are matched, and the first connection protrusions 9111 and the limit grooves 9112 on the adjacent side are used between any two adjacent temperature measurement modules 91. Matching positioning connection. In this embodiment, the first positioning protrusion 9111 , the second positioning protrusion 9211 and the limiting groove 9112 are all dovetail structures.

As shown in FIG. 11 , both ends of the first heat conducting member 913 are respectively mounted on the first bracket 911 through the first heat insulation block 912 , and as shown in FIG. 13 , the upper and lower sides of the first bracket 911 There are heat insulating grooves 9113 on both sides, and the heat insulating grooves 9113 are respectively arranged on both sides of the positioning groove 9112, which can reduce the connection with other parts and reduce the heat transfer. As shown in FIG. 12 , both ends of the second heat-conducting member 923 are respectively mounted on the second bracket 921 through the second heat insulation block 922 , and the lower ends of the legs of the second bracket 921 are provided with heat insulation pads 924 .

In this embodiment, the first heat-conducting member 913 and the second heat-conducting member 923 are made of red copper material. The red copper has excellent heat conduction effect and can quickly transfer heat to the air near it, so that the temperature of the air near it is consistent. The temperature of the accessory air can be accurately reflected under the external infrared observation device. In the present invention, the temperature measurement modules arranged layer by layer in the height direction (that is, the heat conduction members arranged layer by layer) conduct heat transfer with the air in the entire box The external infrared observation device can accurately reflect the temperature field distribution inside the box 1, and then can monitor the printing and forming temperature of the biological material, and accurately control the temperature inside the box 2. The first heat insulating block 912, the second heat insulating block 922, and the heat insulating pad 924 may be aerogel felt.

As shown in FIGS. 3 to 6 , the cover body 1 includes an upper heat preservation cover 11 and a lower cover plate 18 , wherein the upper heat preservation cover 11 is buckled on the lower cover plate 18 , and the first refrigeration component is arranged on the upper heat preservation cover 11 inside and installed on the lower cover plate 18 . In this embodiment, the first refrigeration assembly includes a first semiconductor refrigeration member 17 and a first cooling member 12, wherein the first cooling member 12 is detachably connected to the lower cover plate 18, and the first semiconductor refrigeration member 17 is embedded Between the first cooling element 12 and the lower cover 18 , the first semiconductor cooling element 17 can be cooled on one side, and the other side can generate a lot of heat, so the first semiconductor cooling element 17 is connected with the first cooling element 12 , using the cooling element to dissipate the heat generated by the first semiconductor refrigeration element 17 to prevent the first semiconductor refrigeration element 17 from being burned out. The first semiconductor refrigeration element 17 is a well-known technology in the art and is a commercially available product. A cooling element 12 can adopt a water head that communicates with external circulating water, and has a simple structure and is easy to process.

As shown in FIGS. 3 to 6 , in this embodiment, the first refrigeration component is fixedly mounted on the lower cover plate 18 through the first fixed pressing plate 14 , wherein the upper side of the first cooling member 12 is connected to the first fixed pressing plate 14 . In contact, both ends of the first fixed pressing plate 14 are respectively fixedly connected to the lower cover plate 18 through first bolts 13 .

As shown in FIGS. 3-6 , there are two sets of first refrigeration components in this embodiment, and the middle part of the upper heat preservation cover 11 and the middle part of the lower cover plate 18 are provided with printed through holes 111 for the biological material to drop in. Two sets of first refrigeration components are arranged on both sides of the through-printing hole 111 , the lower cover 18 is provided with a first thermal insulation bottom plate 15 on both sides, and a second thermal insulation bottom plate 16 in the middle to cooperate with the upper thermal insulation cover 11 . In addition, as shown in FIG. 2 , one side of the upper heat preservation cover 11 is provided with a connecting through hole for the joint of the first refrigeration assembly to pass through.

As shown in FIG. 6 , the underside of the lower cover 18 is provided with a cover protrusion 19 for engaging and connecting with the adjacent connecting shell 22 on the box body 2 , and both sides of the lower cover 18 cover the connection The cavities 211 on both sides of the casing 22 ensure its sealing.

As shown in FIGS. 14 to 16 , the base 3 includes a heat insulating shell and a mounting plate 35 , wherein the heat insulating shell includes a heat insulating side plate 31 and a heat insulating top plate 32 , and the upper end of the heat insulating side plate 31 is installed by a third bolt 33 . On the edge of the thermal insulation top plate 32 , a mounting plate 35 is arranged on the lower side of the thermal insulation top plate 32 , the second refrigeration component is arranged in the thermal insulation casing and is detachably mounted on the mounting plate 35 , and one end of the thermal insulation casing is provided with an opening For the joint of the second refrigeration assembly to pass through, in this embodiment, the second refrigeration assembly has the same composition as the first refrigeration assembly, including a second semiconductor refrigeration element 39 and a second cooling element 36, both of which only have size and power, etc. The parameters are different.

As shown in FIG. 15 , a mounting and positioning groove 34 is provided on the upper side of the mounting plate 35 for cooperating with the connecting shell 22 adjacent to the box body 2 , and a mounting opening is provided on the thermal insulation top plate 32 for the The installation and positioning groove 34 passes through, and the thermal insulation top plate 32 is in contact with the lower side of the adjacent connecting shell 22 on the box body 2 to ensure its internal cavity is sealed. In addition, the installation opening allows the cold air generated by the second refrigeration assembly to pass through. into box 2. In addition, in this embodiment, the second refrigeration assembly is fixedly mounted on the lower side of the mounting plate 35 through the second fixed pressing plate 38 , and the two ends of the second fixed pressing plate 38 are respectively connected to the mounting plate 35 by the second bolts 37 . For fixed connection, the second semiconductor refrigeration element 39 in the second refrigeration assembly is embedded between the second cooling element 36 and the mounting plate 35 .

As shown in Figures 17-18, the temperature-controlled print head 4 includes a print head 401, a temperature-controlled jacket 402, and a print head mounting plate 405, wherein the end of the print head 401 is fixedly mounted on the print head mounting plate 405. In the embodiment, the printing mounting plate 405 is installed on an XYZ three-direction moving mechanism to drive the temperature-controlled print head 4 to adjust the position, the temperature-control jacket 402 is fitted on the print head 401, and the temperature-controlled jacket 402 is internally provided. There is a temperature control cavity, and the temperature control jacket 402 is provided with a water inlet 403 and a water outlet 404 to communicate with the temperature control cavity, and a touch sensor insertion port 406 and a heating element are arranged on one side of the temperature control jacket 402 Rod insertion port 407, wherein after the tactile sensor is inserted into the tactile insertion port 406, the detection end of the head end enters the temperature control cavity, and the heating rod of the head end enters the temperature control cavity after the heating rod is inserted into the heating rod insertion port 407 in the body. The temperature-controlled water enters and exits through the water inlet 403 and the water outlet 404, and the touch sensor detects the temperature of the temperature-controlled water in real time, and adjusts it by heating with a heating rod, so as to ensure that the internal temperature of the printing nozzle 401 is above the gel temperature, so that the internal Bioink is in liquid state. The printing nozzle 401 , the touch sensor, the heating rod, and the XYZ three-direction moving mechanism are all known in the art and are commercially available products.

As shown in FIGS. 19 to 22 , the temperature control feeding pipe 7 includes an external temperature control pipe 701 and a feeding pipe 702 arranged in the external temperature control pipe 701 . The outer wall of the feeding pipe 702 is connected to the external temperature control pipe 701 A water bath flow channel 703 is formed between the inner walls of the control tube 701, and the feeding tube 702 communicates with the silo 6 to transport the biological material to the temperature-controlled print head 4, and in order to ensure that the temperature of the biological material is at the gel temperature during the transmission process Above, the water bath flow channel 703 is communicated with the water bath temperature control box 5, and the water temperature in the water bath flow channel 703 is controlled and adjusted by the water bath temperature control box 5, thereby ensuring that the temperature inside the material conveying pipe 702 meets the requirements. The water bath temperature control box 5 is a well-known technology in the art and a commercially available product.

As shown in FIGS. 21-22 , in this embodiment, both ends of the temperature control feeding pipe 7 are provided with connecting joints 704 , and one side of the connecting joint 704 is provided with a first connecting portion 7041 and the external temperature control pipe 701 is connected, the other side is provided with a second connecting part 7042 and a gland 706 is screwed to achieve sealing, the feeding pipe 702 passes through the connecting joint 704, and the connecting joint 704 at the input end of the temperature-controlling feeding pipe 7 is connected to an inlet. The water pipe 705 is connected, the connection joint 704 of the output end of the temperature control feeding pipe 7 is connected to a water outlet pipe 707, and the water flows into the water bath channel 703 through the water inlet pipe 705 and the first connection part 7041 of the input end connection joint 704 in turn, and flows out through the first connecting part 7041 of the output end connecting joint 704 and the water outlet pipe 707 , the second connecting part 7042 is sealed with the gland 706 to prevent water leakage, and the outer end of the second connecting part 7042 is connected with the gland 704 . A sealing ring is arranged between the bottoms of the grooves 706 , and the water inlet pipe 705 and the water outlet pipe 707 are both communicated with the water bath temperature control box 5 .

As shown in FIG. 1 , each part of the present invention is mounted on a workbench 8 .

The working principle of the present invention is:

When the present invention works, the cover body 1 of the temperature control device and the refrigeration components in the base 3 simultaneously cool the inside of the box body 2, which can greatly reduce the temperature gradient inside the entire device, so that the low temperature inside the entire temperature control device tends to be uniform. At this time, when the high-temperature liquid biological material is dropped from the printing through hole 111 of the cover body 1 into the temperature control device, it exchanges heat with the air, and solidifies into a solid biological material and falls on the printing structure inside the temperature control device. 4. Move according to the system setting, so that the biological material is accumulated layer by layer after dripping into the temperature control device to form a biological product with a special shape. In addition, the present invention uses the temperature measuring component 9 to cooperate with the infrared observation device to more accurately reflect the temperature inside the box 2. Therefore, the actual temperature inside the box 2 can be precisely controlled to enhance the printing effect.

The box body 2 of the temperature control device of the present invention and the temperature measuring assembly 9 in it both include a plurality of modules that are sequentially fitted and stacked in the height direction, which can be adjusted according to printing needs, and have a wider application range. In addition, in order to ensure the printing head The temperature of the biological material inside 401 and during the transmission process, the present invention designs a temperature-controlled printing head 4 and a temperature-controlled feeding tube 7, wherein the temperature-controlled printing head 4 is provided with a temperature-controlled jacket 402 outside the printing nozzle 401 to realize the internal temperature of the printing nozzle 401. The temperature control feeding pipe 7 is provided with an external temperature control pipe 701 outside the feeding pipe 702. The water bath channel 703 between the feeding pipe 702 and the external temperature control pipe 701 and the water bath temperature control box 5 Connected to achieve thermal insulation and temperature regulation during biomaterial transport.

Claims (8)

1. An accurate temperature-controlled biological 3D printing system, characterized in that it comprises a temperature-controlled device, a temperature-controlled print head (4), a silo (6) and a temperature-controlled feed pipe (7), wherein the temperature-controlled print head ( 4) Removably arranged on the upper side of the temperature control device, the silo (6) is placed in a water bath temperature control box (5) and connected to the temperature control print head (4) through a temperature control feeding pipe (7), The temperature control device comprises a cover body (1), a box body (2) and a base (3) arranged in sequence from top to bottom, and a first refrigeration component is arranged in the cover body (1), and the base ( 3) A second refrigeration component is provided inside, an infrared observation window (25) is arranged on the side wall of the box body (2), and a temperature measurement component (9) is arranged inside the box body (2) corresponding to the infrared observation window (25); The box body (2) includes a plurality of connection shells (22) that are sequentially fitted and stacked in the height direction, and the temperature measurement assembly (9) includes a temperature measurement base (92) and a plurality of temperature measurement modules (91), And each temperature measurement module (91) is sequentially fitted and superimposed along the height direction; The connection housing (22) is provided with a connection positioning groove (24) at the upper end and a connection positioning projection at the lower end, and a heat preservation sleeve (21) is sheathed on the outer side of the connection housing (22), and the heat preservation sleeve ( 21) Both side walls are provided with cavities (211), each layer of thermal insulation jackets (21) is stacked in sequence with each layer of the connecting shell (22), and the cavities inside each thermal insulation jacket (21) after stacking (211) communicate in sequence; the temperature measurement module (91) includes a first bracket (911) and a first heat conducting member (913), and a first connection protrusion (9111) is provided on the upper side of the first bracket (911) ), a limit groove (9112) is arranged on the lower side, the temperature measuring base (92) includes a second bracket (921) and a second heat conducting member (923), and the upper side of the second bracket (921) is provided with The second connecting protrusion (9211). 2 . The precise temperature-controlled biological 3D printing system according to claim 1 , wherein both ends of the first heat conducting member ( 913 ) are respectively mounted on the first bracket through a first heat insulation block ( 912 ). 3 . (911), and the upper and lower sides of the first bracket (911) are provided with heat insulating grooves (9113), and the two ends of the second heat conducting member (923) pass through the second heat insulating blocks (922) respectively. The second bracket (921) is installed on the second bracket (921), and the lower end of each support foot of the second bracket (921) is provided with a heat insulation pad (924). 3. The precise temperature-controlled biological 3D printing system according to claim 1, wherein a lower cover plate (18) is provided on the lower side of the cover body (1), and the first refrigeration component passes through the first The fixed pressing plate (14) is fixedly mounted on the lower cover plate (18), the first refrigeration assembly includes a first semiconductor refrigeration member (17) and a first cooling member (12), and the first semiconductor refrigeration member (17) ) is embedded between the first cooling element (12) and the lower cover plate (18); the base (3) is provided with a mounting plate (35), and the second refrigeration assembly passes through the second fixed pressure plate (38) is fixedly mounted on the mounting plate (35), the second refrigeration assembly includes a second semiconductor refrigeration element (39) and a second cooling element (36), and the second semiconductor refrigeration element (39) is embedded between the second cooling element (36) and the mounting plate (35). 4. The precise temperature-controlled biological 3D printing system according to claim 1 or 3, wherein the cover body (1) comprises an upper heat preservation cover (11) and a lower cover plate (18), wherein the upper heat preservation cover (11) is buckled on the lower cover (18), the first refrigeration component is arranged in the upper thermal insulation cover (11) and mounted on the lower cover (18), the middle part of the upper thermal insulation cover (11) and the The middle part of the lower cover plate (18) is provided with a printing through hole (111), one side of the upper heat preservation cover (11) is provided with a connecting through hole for the first refrigeration component joint to pass through, and the lower cover plate (111) 18) The lower side is provided with a cover plate protrusion (19). 5. The precise temperature-controlled biological 3D printing system according to claim 1 or 3, characterized in that: the base (3) comprises a thermal insulation shell and a mounting plate (35), wherein the thermal insulation shell comprises a middle part with a mounting plate (35). an open thermal insulation top plate (32), a mounting plate (35) is mounted on the lower side of the thermal insulation top plate (32), a second refrigeration assembly is arranged in a thermal insulation shell and mounted on the mounting plate (35), the thermal insulation shell One end is provided with an opening for the second refrigeration assembly joint to pass through, and the upper side of the mounting plate (35) is provided with a mounting opening through which a mounting and positioning groove (34) passes through the middle of the thermal insulation top plate (32). 6. The precise temperature-controlled biological 3D printing system according to claim 1, wherein the temperature-controlled printing head (4) comprises a printing nozzle (401), a temperature-controlled jacket (402) and a printing head mounting plate ( 405), wherein the tail end of the print head (401) is fixedly mounted on the print head mounting plate (405), the temperature control jacket (402) is sleeved on the print head (401), and the temperature control jacket (402) A temperature control cavity is provided inside, and the temperature control jacket (402) is provided with a water inlet (403) and a water outlet (404) that communicate with the temperature control cavity, and one side of the temperature control jacket (402) is provided. A sensor insertion port (406) and a heating rod insertion port (407) are provided. 7. The precise temperature-controlled biological 3D printing system according to claim 1, wherein the temperature-controlled feeding pipe (7) comprises an external temperature-controlled pipe (701) and an external temperature-controlled pipe (701). ) in the feeding pipe (702), a water bath channel (703) is formed between the outer wall of the feeding pipe (702) and the inner wall of the external temperature control pipe (701). The silo (6) is communicated, and the water bath flow channel (703) is communicated with the water bath temperature control box (5). 8. The precise temperature-controlled biological 3D printing system according to claim 7, wherein both ends of the temperature-controlled feeding tube (7) are provided with connecting joints (704), and the connecting joints (704) are one A first connection part (7041) is provided on one side to connect with the external temperature control pipe (701), and a second connection part (7042) is provided on the other side to be sealed and connected to a gland (706). The feeding pipe (702) Passing through the connecting joint (704), the connecting joint (704) at the input end of the temperature-controlled feeding pipe (7) is connected with a water inlet pipe (705), and the connecting joint (704) at the output end of the temperature-controlled feeding pipe (7) ) is connected with a water outlet pipe (707), and the water inlet pipe (705) and the water outlet pipe (707) are both communicated with the water bath temperature control box (5).

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