CN113957738B - Method for removing endotoxin in bacterial cellulose - Google Patents
Method for removing endotoxin in bacterial cellulose Download PDFInfo
- Publication number
- CN113957738B CN113957738B CN202111253607.7A CN202111253607A CN113957738B CN 113957738 B CN113957738 B CN 113957738B CN 202111253607 A CN202111253607 A CN 202111253607A CN 113957738 B CN113957738 B CN 113957738B
- Authority
- CN
- China
- Prior art keywords
- bacterial cellulose
- endotoxin
- sodium hydroxide
- soaking
- hydroxide solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/08—Removal of fats, resins, pitch or waxes; Chemical or physical purification, i.e. refining, of crude cellulose by removing non-cellulosic contaminants, optionally combined with bleaching
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/08—Removal of fats, resins, pitch or waxes; Chemical or physical purification, i.e. refining, of crude cellulose by removing non-cellulosic contaminants, optionally combined with bleaching
- D21C9/083—Removal of fats, resins, pitch or waxes; Chemical or physical purification, i.e. refining, of crude cellulose by removing non-cellulosic contaminants, optionally combined with bleaching with inorganic compounds
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/08—Removal of fats, resins, pitch or waxes; Chemical or physical purification, i.e. refining, of crude cellulose by removing non-cellulosic contaminants, optionally combined with bleaching
- D21C9/086—Removal of fats, resins, pitch or waxes; Chemical or physical purification, i.e. refining, of crude cellulose by removing non-cellulosic contaminants, optionally combined with bleaching with organic compounds or compositions comprising organic compounds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to the technical field of medical equipment, in particular to a method for removing endotoxin in bacterial cellulose, which comprises the following steps: (1) Soaking the sliced bacterial cellulose in sodium hydroxide solution, and replacing the sodium hydroxide solution for multiple soaking; (2) Soaking bacterial cellulose in ethanol solution to dissolve endotoxin in the porous structure; (3) Adding the bacterial cellulose treated in the step (2) into sodium hydroxide solution and polysulfone resin polymer with positive charges; (4) The treated bacterial cellulose was soaked with water for injection to a pH in the range of 7.+ -. 1. The method of the invention not only removes endotoxin in the bacterial cellulose, but also quickens the purification process of the bacterial cellulose, has no influence on the mechanical property and the physicochemical aspect of the bacterial cellulose, and the endotoxin content of the obtained bacterial cellulose is far less than the specified content.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a method for removing endotoxin in bacterial cellulose.
Background
The disclosure of this background section is only intended to increase the understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art.
At present, three products of medical instruments have strict control on endotoxin, bacterial cellulose has high value as a novel natural nano material, and the bacterial cellulose has good air permeability and good biocompatibility and has wide prospect in the medical industry. However, endotoxin removal is an immature direction in the industry of bacterial cellulose materials, and bacterial cellulose has a porous structure, so that endotoxin cannot be easily discharged, and thus, the endotoxin removal by the conventional method is difficult. For this we start from the physical properties of endotoxin, which by some physical properties led out the bacterial cellulose interior endotoxin for further removal.
Endotoxemia, also known as lipopolysaccharide or LPS, is a component on the membrane of gram-negative bacteria. The outer lipid component of the bacterial outer membrane is entirely composed of endotoxin molecules. Each LPS molecule in turn consists of a hydrophobic lipid a, a complex polysaccharide chain and negatively charged phosphate groups. Thus each endotoxin contains both hydrophobic and hydrophilic and charged regions, giving it unique properties of interaction with other molecules. Bacteria have fewer endotoxin components on their surface as they grow actively, and release significant amounts of endotoxin once they die. During lysis of plasmid extraction, endotoxins are released from the outer membrane of the bacteria into the lysate. The chemical composition of the composition is phospholipid polysaccharide-protein complex, the structure of the composition comprises 3 areas, namely a lipoid A area, a core polysaccharide area and a specific polysaccharide area, and the toxic composition of the composition is mainly lipoid A, so that the composition is one of pyrogens. Very small amounts of endotoxin enter the animal or human body and trigger a strong inflammatory response, resulting in severe consequences of death, such as mild fever and severe death. The conventional methods for removing endotoxin include activated carbon adsorption, extraction, ultrafiltration, ion exchange chromatography and the like, but the removal effect is poor.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide a method for removing endotoxin in bacterial cellulose, which is not a mature method at present, and can be used for filtering by adopting endotoxin physical characteristics aiming at the characteristics of a bacterial cellulose porous structure, and endotoxin is negatively charged under the common pH condition.
In order to achieve the above object, the technical scheme of the present invention is as follows:
in a first aspect of the invention, there is provided a method of removing endotoxin from bacterial cellulose, the method comprising the steps of:
(1) Soaking the sliced bacterial cellulose in sodium hydroxide solution, and replacing the sodium hydroxide solution for multiple soaking;
(2) Soaking bacterial cellulose in ethanol solution to dissolve endotoxin in the porous structure;
(3) Adding the bacterial cellulose treated in the step (2) into sodium hydroxide solution and polysulfone resin polymer with positive charges, wherein the polysulfone resin polymer absorbs and filters out endotoxin in liquid and bacterial cellulose, and the sodium hydroxide solution completely kills the absorbed endotoxin;
(4) The treated bacterial cellulose was soaked to a pH in the range of 7.+ -. 1.
Bacterial cellulose is a porous structure, bacteria are not easy to wash out, and endotoxin is not easy to come out. The sodium hydroxide solution is used for soaking the bacterial cellulose to enable internal bacteria to be cracked to release endotoxin, and the sodium hydroxide solution with the concentration is used for accelerating the cracking of the endotoxin in the bacterial cellulose and accelerating the bacterial cellulose purification process.
The soaked bacterial cellulose is soaked by ethanol and sodium hydroxide solution, and the ethanol can promote the dissolution of endotoxin in the bacterial cellulose by using a similar compatibility principle, and the dissolved endotoxin is removed by using the sodium hydroxide solution and the charge adsorption. The most conserved part of endotoxin is phospholipid a; the inner core region is similar to the conservation of lipid a, and the outer core region has a high degree of variation. The inner core region, KDO and phospholipid a themselves carry a large number of phosphate groups as substitutes and thus make the endotoxin negatively charged; the surface of the filter has positive charges, so that endotoxin in liquid and bacterial cellulose can be effectively adsorbed and filtered, and endotoxin in solution can be effectively adsorbed while precise filtration is performed, and the dual functions of filtration and adsorption are realized. The polysulfone resin polymer has good hydrophilicity, can be quickly wetted and filtered, and has excellent flow rate and high flux; if quaternized, positively charged, endotoxin in bacterial cellulose can be removed by charge adsorption.
The specific embodiment of the invention has the following beneficial effects:
the bacterial cellulose is soaked in 1% -3% sodium hydroxide solution, endotoxin is dissolved out by 5% -15% ethanol, the pH value of the water for injection is regulated to 7+/-1 by 1% -3% sodium hydroxide solution and positive charge adsorption, the series of operations not only remove the endotoxin in the bacterial cellulose, but also quicken the purification process of the bacterial cellulose, the mechanical property and physicochemical aspects of the bacterial cellulose are not influenced, and the content of the obtained bacterial cellulose endotoxin is far less than the specified content, so that the method is suitable for removing the endotoxin in the bacterial cellulose industry.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
In one embodiment of the present invention, there is provided a method for removing endotoxin from bacterial cellulose, the method comprising the steps of:
(1) Soaking the sliced bacterial cellulose in sodium hydroxide solution, and replacing the sodium hydroxide solution for multiple soaking;
(2) Soaking bacterial cellulose in ethanol solution to dissolve endotoxin in the porous structure;
(3) Adding the bacterial cellulose treated in the step (2) into sodium hydroxide solution and polysulfone resin polymer with positive charges, wherein the polysulfone resin polymer absorbs and filters out endotoxin in liquid and bacterial cellulose, and the sodium hydroxide solution completely kills the absorbed endotoxin;
(4) The treated bacterial cellulose was soaked to a pH in the range of 7.+ -. 1.
In one or more embodiments, the sodium hydroxide solution in step (1) has a mass concentration of 1% to 3%;
preferably, the soaking times are not less than 4 times, and each soaking time is 3-5 hours; thus, bacteria are killed, so that endotoxin is completely released, and meanwhile, the purification process of bacterial cellulose is quickened;
preferably, the number of times of soaking is 4, and each time of soaking is 4 hours;
in one or more embodiments, step (1) is operated in an environment above the hundred thousand levels, avoiding further contamination;
in one or more embodiments, in step (2), the concentration of the ethanol solution is 5% to 15% by volume; the soaking time is as follows: 1-3 hours;
the bacterial cellulose after purification is soaked in sodium hydroxide solution to kill part of endotoxin and gram-negative bacteria, and release part of endotoxin. The elimination of endotoxins is not complete based on bacterial cellulose porous structures. For this, bacterial cellulose is soaked in 5% -15% ethanol, and endotoxin chemical composition is phospholipid polysaccharide-protein complex, its structure contains 3 regions, namely lipid A region, core polysaccharide region and specific polysaccharide region, its toxic composition is mainly lipid A, and it is easily dissolved in ethanol solution, and 5% -15% ethanol is used to dissolve out endotoxin in porous structure.
In one or more embodiments, in step (3), the sodium hydroxide solution has a mass concentration of 1% to 3% sodium hydroxide solution;
the most conserved portion of endotoxin is phospholipid a. The inner core region is similar to the conservation of lipid a, and the outer core region has a high degree of variation. The inner core region, KDO and phospholipid a, themselves carry a large number of phosphate groups as substitutes and thus make the endotoxin negatively charged. Aiming at the characteristic that endotoxin is negatively charged, the bacterial cellulose treated in the second step is added with 1% -3% sodium hydroxide solution and positively charged polysulfone resin polymer, the polysulfone resin polymer has better hydrophilicity, and can effectively adsorb and filter endotoxin in liquid and bacterial cellulose, and the 1% -3% sodium hydroxide solution can completely kill the adsorbed endotoxin.
In one or more embodiments, the soaking in step (4) is performed using water for injection.
The invention is further illustrated and described below in connection with specific examples.
Example 1
(1) Soaking the sliced bacterial cellulose in 2% sodium hydroxide solution by mass fraction, and replacing the sodium hydroxide solution four times for four hours each time; killing bacteria to completely release endotoxin and accelerate the purification process of bacterial cellulose;
(2) Soaking bacterial cellulose in 10% ethanol by volume fraction, and dissolving endotoxin in the porous structure by using 10% ethanol;
(3) The bacterial cellulose treated in the second step is added with a sodium hydroxide solution with the mass fraction of 2% and a polysulfone resin polymer with positive charges, the polysulfone resin polymer has better hydrophilicity, and can effectively adsorb and filter endotoxin in liquid and bacterial cellulose, and the endotoxin adsorbed by the sodium hydroxide solution with the mass fraction of 2% can be completely killed;
(4) The treated bacterial cellulose was soaked with water for injection to a ph of 7.+ -. 1.
(5) Randomly extracting samples, detecting endotoxin by using a gel method, and testing mechanical properties.
Example 2
(1) Soaking the sliced bacterial cellulose in 3% sodium hydroxide solution by mass percent, and replacing the sodium hydroxide solution four times for four hours each time; killing bacteria to completely release endotoxin and accelerate the purification process of bacterial cellulose;
(2) Soaking bacterial cellulose in 15% ethanol by volume fraction, and dissolving endotoxin in the porous structure by using 15% ethanol;
(3) Adding 3% sodium hydroxide solution and positively charged polysulfone resin polymer into the bacterial cellulose treated in the second step, wherein the polysulfone resin polymer has good hydrophilicity, can effectively adsorb and filter endotoxin in liquid and bacterial cellulose, and the 3% sodium hydroxide solution can completely kill the adsorbed endotoxin;
(4) Soaking the treated bacterial cellulose in water for injection to reach the pH of 7+/-1;
(5) Randomly extracting samples, detecting endotoxin by using a gel method, and testing mechanical properties.
Example 3
(1) Soaking the sliced bacterial cellulose in a sodium hydroxide solution with the mass fraction of 1%, replacing the sodium hydroxide solution four times, and killing bacteria after soaking for four hours each time to completely release endotoxin and accelerate the purification process of the bacterial cellulose;
(2) Soaking bacterial cellulose in 5% ethanol by volume fraction, and dissolving endotoxin in the porous structure by using 5% ethanol;
(3) Adding the bacterial cellulose treated in the step (2) into a 1% sodium hydroxide solution and a polysulfone resin polymer with positive charges, wherein the polysulfone resin polymer has good hydrophilicity, can effectively adsorb and filter endotoxin in liquid and bacterial cellulose, and the 1% sodium hydroxide solution can completely kill the adsorbed endotoxin;
(4) Soaking the treated bacterial cellulose in water for injection to reach the pH value of 7+/-1;
(5) Randomly extracting samples, detecting endotoxin by using a gel method, and testing mechanical properties.
Comparative example 1
(1) Soaking the sliced bacterial cellulose in a sodium hydroxide solution with the mass fraction of 1%, replacing the sodium hydroxide solution four times, and killing bacteria after soaking for four hours each time to completely release endotoxin and accelerate the purification process of the bacterial cellulose;
(2) Soaking bacterial cellulose in 5% ethanol by volume fraction, and dissolving endotoxin in the porous structure by using 5% ethanol;
(3) Soaking the treated bacterial cellulose in water for injection to reach the pH value of 7+/-1;
(4) Randomly extracting samples, detecting endotoxin by using a gel method, and testing mechanical properties.
Comparative example 2
(1) Soaking the sliced bacterial cellulose in 2% sodium hydroxide solution by mass fraction for four times, wherein each soaking time is four hours, killing bacteria to completely release endotoxin and simultaneously accelerating the purification process of the bacterial cellulose;
(2) Soaking bacterial cellulose in 10% ethanol by volume fraction, and dissolving endotoxin in the porous structure by using 10% ethanol;
(3) Adding the bacterial cellulose treated in the step (2) into a sodium hydroxide solution with the mass fraction of 2%, and killing adsorbed endotoxin;
(4) Soaking the treated bacterial cellulose in water for injection to reach the pH value of 7+/-1;
(5) Randomly extracting samples, detecting endotoxin by using a gel method, and testing mechanical properties.
Comparative example 3
(1) Soaking the sliced bacterial cellulose in 10% ethanol by volume fraction, and dissolving endotoxin in the porous structure by using 10% ethanol;
(2) Adding the bacterial cellulose treated in the step (1) into a 2% sodium hydroxide solution and a polysulfone resin polymer with positive charges, wherein the polysulfone resin polymer has good hydrophilicity, can effectively adsorb and filter endotoxin in liquid and bacterial cellulose, and the 2% sodium hydroxide solution can completely kill the adsorbed endotoxin;
(3) Soaking the treated bacterial cellulose in water for injection to reach the pH value of 7+/-1;
(4) Randomly extracting samples, detecting endotoxin by using a gel method, and testing mechanical properties.
The results of endotoxin content detection and mechanical property test of the bacterial celluloses obtained in examples 1 to 3 and comparative examples 1 to 3 are shown in Table 1:
TABLE 1
As can be seen from Table 1, the series of operations of soaking in 1% -3% sodium hydroxide solution, dissolving out endotoxin in 5% -15% ethanol, regulating ph to 7+ -1% by 1% -3% sodium hydroxide solution and positive charge adsorption and detecting endotoxin not only remove endotoxin in bacterial cellulose but also accelerate the purification process of bacterial cellulose, and the toughness and physicochemical aspects of bacterial cellulose are not affected, so that the content of the obtained bacterial cellulose endotoxin is far less than the specified content, and therefore, the method is suitable for removing endotoxin in bacterial cellulose industry.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A method for removing endotoxins from bacterial cellulose, said method comprising the steps of:
(1) Soaking the sliced bacterial cellulose in sodium hydroxide solution, and replacing the sodium hydroxide solution for multiple soaking;
(2) Soaking bacterial cellulose in ethanol solution to dissolve endotoxin in the porous structure;
(3) Adding the bacterial cellulose treated in the step (2) into sodium hydroxide solution and polysulfone resin polymer with positive charges, wherein the polysulfone resin polymer absorbs and filters out endotoxin in liquid and bacterial cellulose, and the sodium hydroxide solution completely kills the absorbed endotoxin;
(4) Soaking the treated bacterial cellulose to reach the pH value of 7+/-1;
the mass concentration of the sodium hydroxide solution in the step (1) is 1% -3%;
in the step (2), the concentration of the ethanol solution is 5% -15%; the soaking time is 1-3 hours;
in the step (3), the mass concentration of the sodium hydroxide solution is 1% -3% of the sodium hydroxide solution.
2. The method for removing endotoxin from bacterial cellulose as claimed in claim 1, wherein the number of times of soaking in step (1) is not less than 4 and each time of soaking is 3 to 5 hours.
3. The method for removing endotoxin from bacterial cellulose as claimed in claim 2, wherein the number of times of soaking in step (1) is 4, each for 4 hours.
4. The method for removing endotoxins from bacterial cellulose according to claim 1, wherein the soaking in step (4) is performed using water for injection.
5. The method of removing endotoxins from bacterial cellulose as recited in claim 1, wherein step (1) is operated in an environment above the hundred thousand levels.
6. The method for removing endotoxins from bacterial cellulose as recited in claim 1, wherein in step (2), the concentration of said ethanol solution is 15%; the soaking time was 2 hours.
7. The method for removing endotoxin from bacterial cellulose as claimed in claim 1, wherein in step (3), the mass concentration of said sodium hydroxide solution is 3% sodium hydroxide solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111253607.7A CN113957738B (en) | 2021-10-27 | 2021-10-27 | Method for removing endotoxin in bacterial cellulose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111253607.7A CN113957738B (en) | 2021-10-27 | 2021-10-27 | Method for removing endotoxin in bacterial cellulose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113957738A CN113957738A (en) | 2022-01-21 |
| CN113957738B true CN113957738B (en) | 2023-05-09 |
Family
ID=79467488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111253607.7A Active CN113957738B (en) | 2021-10-27 | 2021-10-27 | Method for removing endotoxin in bacterial cellulose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113957738B (en) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102924613A (en) * | 2012-11-01 | 2013-02-13 | 海南大学 | Preparation method of dialdehyde bacterial cellulose |
| CN102977392A (en) * | 2012-12-17 | 2013-03-20 | 上海典范医疗科技有限公司 | Preparation method of bacterial cellulose capable of removing endotoxin |
| CN107022033B (en) * | 2017-05-25 | 2019-03-29 | 天津科技大学 | A kind of method of high-performance flash purification bacteria cellulose |
| CN107441964A (en) * | 2017-08-31 | 2017-12-08 | 上海振浦医疗设备有限公司 | A kind of endotoxin filters out film and preparation method thereof |
| CN108329507A (en) * | 2018-03-05 | 2018-07-27 | 涂青山 | A method of reducing endotoxin amount in bacteria cellulose moulding material |
| CN108532343A (en) * | 2018-04-20 | 2018-09-14 | 北京理工大学珠海学院 | A kind of method for purifying and separating of bacteria cellulose |
| CN109914141B (en) * | 2019-03-05 | 2021-05-14 | 华东交通大学 | Method for purifying and separating bacterial cellulose |
-
2021
- 2021-10-27 CN CN202111253607.7A patent/CN113957738B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113957738A (en) | 2022-01-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2389242B1 (en) | Sorbent for endotoxins | |
| US6451772B1 (en) | Biopolymer salts with low endotoxin levels, biopolymer compositions thereof and methods of making the same | |
| AT509192B1 (en) | SORPTION FOR ENDOTOXINES | |
| CN109173746A (en) | The compound membrane preparation method of micropollutants in a kind of high efficiency filter water | |
| US5136032A (en) | Method for separating phosphopolyol compounds using a separating agent | |
| DE102006055558A1 (en) | Adsorbent for removing endotoxins from solutions comprises a ligand in the form of an amine-functional molecule in which some of the amine groups are modified with an amine-reactive molecule | |
| CN113957738B (en) | Method for removing endotoxin in bacterial cellulose | |
| US6106723A (en) | Method for removing pyrogens from dialysate | |
| WO2021133332A2 (en) | A novel composite membrane in nanofiber form for selectively separating anionic and cationic organic compounds, and a preparation method thereof | |
| DE10258944A1 (en) | Device for removing bacterial toxins from blood or plasma, useful for treating sepsis, also for analysis and diagnosis, includes hollow fiber material for selective binding of the toxins | |
| KR20140129742A (en) | Hollow fiber membrane for removing virus and preparation method thereof | |
| Pratiwi et al. | Enhanced antibiofouling properties of chitosan-based membranes by coating and blending of Moringa Oleifera L extracts | |
| Yamamoto et al. | Endotoxin rejection by ultrafiltration through high‐flux, hollow fiber filters | |
| JP2017018842A (en) | Hydrophilic polymer adsorbent material and water treatment method using the same | |
| CN112568303B (en) | Instant tea preparation process based on microfiltration-reverse osmosis technology | |
| JP3933300B2 (en) | Polysulfone selective separation membrane | |
| DE4209988A1 (en) | Endotoxin adsorber having high binding capacity - comprises polyethyleneimine bonded to porous carrier, esp. polysaccharide | |
| EP1602387B1 (en) | Device for removal of bacterial lipopolysaccharides and/or lipoteichonic acids from liquids containing proteins and use for the manufacture of means for the removal of these compounds | |
| Madsen et al. | Hemodialysis membrane surface chemistry as a barrier to lipopolysaccharide transfer | |
| Roshni et al. | Synthesis of ceramic tubular membrane from low‐cost clay precursors for blood purification application as substitute of commercial dialysis membrane | |
| US6506308B1 (en) | Methods for the removal of organic substances from aqueous solutions | |
| WO2000016897A1 (en) | Microfiltration filter layer for separating endotoxins and the use of said microfiltration filter layer | |
| KR0149793B1 (en) | Purification method of high molecular hydraulic acid | |
| Pathak et al. | Cu (II)-cross-linked chitosan membrane (CCCM): preparation, characterization and urea removal study using the diffusion-cell model (DCM) | |
| RU2620115C1 (en) | Sorbents for isolation of inorganic endotoxin salts from water and aquous solutions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Method for Removing Endotoxins from Bacterial Cellulose Effective date of registration: 20230904 Granted publication date: 20230509 Pledgee: Ji'nan finance Company limited by guarantee Pledgor: SHANDONG NAMEIDE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023980055212 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |