CN113951286A - Components of disinfectant for pathogenic microorganism experiments and preparation method thereof - Google Patents
Components of disinfectant for pathogenic microorganism experiments and preparation method thereof Download PDFInfo
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- CN113951286A CN113951286A CN202111223374.6A CN202111223374A CN113951286A CN 113951286 A CN113951286 A CN 113951286A CN 202111223374 A CN202111223374 A CN 202111223374A CN 113951286 A CN113951286 A CN 113951286A
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- A—HUMAN NECESSITIES
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- A61K36/88—Liliopsida (monocotyledons)
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- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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Abstract
The invention relates to the technical field of disinfectant preparation, and discloses components of a disinfectant for pathogenic microorganism experiments, which comprise the following components in percentage by weight: 20 to 35 percent of aloe, 15 to 19 percent of folium artemisiae argyi, 10 to 13 percent of sow thistle, 8 to 15 percent of garlic, 7 to 11 percent of onion, 11 to 16 percent of radix sophorae flavescentis and 15 to 20 percent of lemon; the aloe, the folium artemisiae argyi, the sow thistle, the garlic, the onion, the radix sophorae flavescentis and the lemon are all herbaceous plants and do not have toxicity, so that the disinfectant prepared by the disinfectant can be used for disinfecting experimental equipment and laboratory air, can not harm a human body after being inhaled by the human body after being used for a long time, can be used for two purposes, can be used for disinfecting the outside of the organism and can also be taken by the organism to perform in-vivo antibacterial disinfection, the lemon can play a role in disinfection, and the lemon can cover heavy odor components such as the garlic and the onion, so that the prepared disinfectant is more fragrant.
Description
Technical Field
The invention relates to the technical field of disinfectant preparation, in particular to components of a disinfectant for pathogenic microorganism experiments and a preparation method thereof.
Background
In order to clarify the pathogenic mechanism of different pathogenic microorganisms to human body, biological laboratory staff usually culture various pathogenic microorganisms in a laboratory and use laboratory animals to perform experiments, so as to clarify the pathogenic mechanism of different pathogenic microorganisms to human body.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the components of the disinfectant for the pathogenic microorganism experiment and the preparation method thereof, and solves the problems in the background technology.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: the components of the disinfectant for pathogenic microorganism experiments comprise the following components in percentage by weight:
20-35% of aloe, 15-19% of folium artemisiae argyi, 10-13% of sow thistle, 8-15% of garlic, 7-11% of onion, 11-16% of radix sophorae flavescentis and 15-20% of lemon.
Preferably, the composition comprises the following components in percentage by weight: 28% of aloe, 17% of folium artemisiae argyi, 10% of sow thistle, 11% of garlic, 8% of onion, 12% of radix sophorae flavescentis and 17% of lemon.
Preferably, the aloe, the folium artemisiae argyi, the sow thistle, the garlic, the onion, the radix sophorae flavescentis and the lemon are respectively aloe juice, folium artemisiae argyi juice, sow thistle juice, garlic juice, onion juice, bitter ginseng juice and lemon juice.
Preferably, the preparation process of the aloe juice comprises the following steps: firstly, selecting full and undamaged aloe leaves, washing the aloe leaves for 2-3 times by using deionized water, cutting the cleaned aloe leaves into small blocks with uniform size, putting the small blocks of the aloe leaves into a crushing device, crushing the small blocks of the aloe leaves for 8-10 min at the rotating speed of 1500-2000 r/min to obtain aloe crude juice, sieving the obtained aloe crude juice by using a 10-20 mesh sieve, putting the filtered aloe crude juice into a centrifugal device, and centrifuging the filtered aloe crude juice for 10-15 min to obtain the aloe juice;
the preparation process of the folium artemisiae argyi juice comprises the following steps: firstly, selecting fresh and undamaged folium artemisiae argyi, cleaning the fresh and undamaged folium artemisiae argyi for 2-3 times by using deionized water, then putting the cleaned folium artemisiae argyi into a container, extruding the folium artemisiae argyi in the container under the pressure of 10-15 MPa, taking out the folium artemisiae argyi in the container after the extrusion is finished, simultaneously sieving the folium artemisiae argyi juice in the container by a sieve of 100-fold and 120-fold, putting the extruded folium artemisiae argyi into the container again, extruding the folium artemisiae argyi again under the pressure of 5-8 MPa, then taking out the folium artemisiae argyi from the container, sieving the folium artemisiae argyi juice in the container by a sieve of 100-fold and 120-fold, uniformly mixing the folium artemisiae argyi juice after the two times of filtration, simultaneously adding 1-2 times of deionized water into the folium artemisiae argyi juice, then adding a decolorizing agent into the folium artemisiae argyi juice, stirring for 5-8 min, and sieving the folium artemisiae argyi juice by a sieve of 80-100-fold to obtain the folium artemisiae argyi juice;
the preparation process of the sow thistle juice comprises the following steps: firstly, selecting fresh and undamaged sow thistle leaves, cleaning the fresh and undamaged sow thistle leaves for 2-3 times by using deionized water, then putting the cleaned sow thistle leaves into a crushing device, crushing the cleaned sow thistle leaves for 5-7 min at the rotating speed of 1500-2000 r/min, then sieving the crushed sow thistle leaves with a 100-mesh sieve and 200-mesh sieve to obtain filtrate, then adding a decolorizing agent into the filtrate, stirring for 10min, and then sieving with a 80-100-mesh sieve again to obtain sow thistle juice;
the preparation process of the garlic juice comprises the following steps: firstly, selecting full and peeled garlic, then putting the garlic into a crushing device, simultaneously adding 1.5-2 times of deionized water into the crushing device, then crushing for 10-15 min at the rotating speed of 1500-2000 r/min, and sieving the crushed garlic with a 120-fold 150-mesh sieve to obtain garlic juice;
the preparation process of the onion juice comprises the following steps: selecting peeled onion, cutting the onion into small particles, putting the small-particle onion into a crushing device, adding deionized water 2-3 times of the onion into the crushing device, crushing for 10-15 min at the rotating speed of 1000-1500 r/min, and sieving the crushed onion with a sieve of 120-150 meshes to obtain onion juice;
the preparation process of the bitter ginseng juice comprises the following steps: firstly, putting the sophora flavescens tablets into a crushing device, crushing for 10min-20min at the rotating speed of 1500r/min-2000r/min to obtain sophora flavescens powder, then adding 2 times of deionized water into the sophora flavescens powder, uniformly stirring, and then sieving by a sieve of 80-120 meshes to obtain sophora flavescens juice;
the preparation process of the lemon juice comprises the following steps: selecting fresh lemons, cleaning the fresh lemons with deionized water, slicing the lemons, removing seeds, putting the lemon slices into a container, and extruding the lemon slices for 10-15 min under the pressure of 10-15 MPa to obtain lemon juice.
The preparation method of the disinfectant for pathogenic microorganism experiments comprises the following steps:
s1, sequentially and respectively adding the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice into a container according to the weight percentage;
s2, stirring the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice in the container uniformly by using a stirring device;
s3, adding lemon juice into the uniformly stirred aloe juice, folium artemisiae argyi juice, sow thistle juice, garlic juice, onion juice and bitter ginseng juice according to the weight percentage, and uniformly stirring by using a stirring device again to obtain a mixture;
s4, packaging and quality testing the mixture obtained in the step S3 to obtain the disinfectant.
(III) advantageous effects
The invention provides components of a disinfectant for pathogenic microorganism experiments and a preparation method thereof, and the disinfectant has the following beneficial effects:
(1) the aloe, the folium artemisiae argyi, the sow thistle, the garlic, the onion, the radix sophorae flavescentis and the lemon are all herbaceous plants and have no toxicity, so that the disinfectant prepared by the method can be used for disinfecting experimental equipment and laboratory air, can not cause harm to a human body after being inhaled by the human body after being used for a long time, can be used for two purposes, can be used for disinfecting the outside of the organism and can be taken by the organism for in vivo bacteriostasis and disinfection.
(2) The lemon is added, so that the lemon can play a role in disinfection, and the heavy-odor components such as garlic and onion can be covered by the lemon, so that the prepared disinfectant has a better faint scent, and the condition that a human body feels uncomfortable to the heavy-odor components such as garlic and onion in the disinfection process is avoided.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The components of the disinfectant for pathogenic microorganism experiments comprise the following components in percentage by weight:
20% of aloe juice, 15% of folium artemisiae argyi juice, 10% of sow thistle juice, 8% of garlic juice, 7% of onion juice, 11% of radix sophorae flavescentis juice and 15% of lemon juice.
The preparation process of the aloe juice comprises the following steps: firstly, selecting full and undamaged aloe leaves, washing the aloe leaves for 2 times by using deionized water, cutting the cleaned aloe leaves into small blocks with uniform size, putting the small blocks of the aloe leaves into a crushing device, crushing the small blocks of the aloe leaves for 8min at the rotating speed of 1500r/min to obtain aloe crude juice, then sieving the obtained aloe crude juice with a 10-mesh sieve, putting the filtered aloe crude juice into a centrifugal device, and centrifuging the filtered aloe crude juice for 10min to obtain the aloe juice;
the preparation process of the folium artemisiae argyi juice comprises the following steps: firstly, selecting fresh and undamaged folium artemisiae argyi, washing the fresh and undamaged folium artemisiae argyi with deionized water for 2 times, then putting the washed folium artemisiae argyi into a container, extruding the folium artemisiae argyi in the container under the pressure of 10MPa, taking out the folium artemisiae argyi in the container after the extrusion is finished, simultaneously sieving the folium artemisiae argyi juice in the container with a 100-mesh sieve, putting the extruded folium artemisiae argyi into the container again, extruding the folium artemisiae argyi again under the pressure of 5MPa, then taking out the folium artemisiae argyi from the container, sieving the folium artemisiae argyi juice in the container with the 100-mesh sieve, uniformly mixing the folium artemisiae argyi juice after the two times of filtration, simultaneously adding 1 time of deionized water into the folium artemisiae argyi juice, then adding a decolorizing agent into the folium artemisiae argyi juice, stirring for 5 minutes, and sieving with the 80-mesh sieve to obtain the folium artemisiae argyi juice;
the preparation process of the sow thistle juice comprises the following steps: firstly, selecting fresh and undamaged sow thistle leaves, washing the fresh and undamaged sow thistle leaves for 2 times by using deionized water, then putting the washed sow thistle leaves into a crushing device, crushing the sow thistle leaves for 5min at the rotating speed of 1500r/min, then sieving the crushed sow thistle leaves by using a 100-mesh sieve to obtain filtrate, then adding a decolorizing agent into the filtrate, stirring the filtrate for 10min, and then sieving the filtrate by using a 80-mesh sieve again to obtain sow thistle juice;
the preparation process of the garlic juice comprises the following steps: firstly, selecting full and peeled garlic, then putting the garlic into a crushing device, simultaneously adding 1.5 times of deionized water into the crushing device, then crushing for 10min at the rotating speed of 1500r/min, and sieving the crushed garlic with a 120-mesh sieve to obtain garlic juice;
the preparation process of the onion juice comprises the following steps: selecting peeled onion, cutting the onion into small particles, putting the small-particle onion into a crushing device, adding deionized water which is 2 times of the onion into the crushing device, crushing for 10min at the rotating speed of 1000r/min, and sieving the crushed onion with a 120-mesh sieve to obtain onion juice;
the preparation process of the bitter ginseng juice comprises the following steps: firstly, putting the sophora flavescens tablets into a crushing device, crushing for 10min at the rotating speed of 1500r/min to obtain sophora flavescens powder, then adding 2 times of deionized water into the sophora flavescens powder, uniformly stirring, and then sieving by a sieve of 80 meshes to obtain sophora flavescens juice;
the preparation process of the lemon juice comprises the following steps: selecting fresh lemons, cleaning the fresh lemons with deionized water, slicing the lemons, removing seeds, putting the lemon slices into a container, and extruding the lemon slices under the pressure of 10MPa for 10min to obtain lemon juice.
The preparation method of the disinfectant for pathogenic microorganism experiments comprises the following steps:
s1, sequentially and respectively adding the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice into a container according to the weight percentage;
s2, stirring the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice in the container uniformly by using a stirring device;
s3, adding lemon juice into the uniformly stirred aloe juice, folium artemisiae argyi juice, sow thistle juice, garlic juice, onion juice and bitter ginseng juice according to the weight percentage, and uniformly stirring by using a stirring device again to obtain a mixture;
s4, packaging and quality testing the mixture obtained in the step S3 to obtain the disinfectant.
Example 2
The components of the disinfectant for pathogenic microorganism experiments comprise the following components in percentage by weight:
28% of aloe juice, 17% of folium artemisiae argyi juice, 11% of sow thistle juice, 12% of garlic juice, 9% of onion juice, 13% of radix sophorae flavescentis juice and 18% of lemon juice.
The preparation process of the aloe juice comprises the following steps: firstly, selecting full and undamaged aloe leaves, washing the aloe leaves for 2 times by using deionized water, cutting the cleaned aloe leaves into small blocks with uniform size, putting the small blocks of the aloe leaves into a crushing device, crushing the small blocks of the aloe leaves for 9min at the rotating speed of 1800r/min to obtain aloe crude juice, then sieving the obtained aloe crude juice with a 15-mesh sieve, putting the filtered aloe crude juice into a centrifugal device, and centrifuging the filtered aloe crude juice for 12min to obtain the aloe juice;
the preparation process of the folium artemisiae argyi juice comprises the following steps: firstly, selecting fresh and undamaged folium artemisiae argyi, washing the fresh and undamaged folium artemisiae argyi with deionized water for 2 times, then putting the washed folium artemisiae argyi into a container, extruding the folium artemisiae argyi in the container under the pressure of 13MPa, taking the folium artemisiae argyi out of the container after the extrusion is finished, simultaneously sieving folium artemisiae argyi juice in the container with a 110-mesh sieve, putting the extruded folium artemisiae argyi into the container again, extruding the folium artemisiae argyi again under the pressure of 6MPa, then taking the folium artemisiae argyi out of the container, sieving the folium artemisiae argyi juice in the container with the 110-mesh sieve, uniformly mixing the folium artemisiae argyi juice after the two times of filtration, simultaneously adding 1.5 times of deionized water into the folium artemisiae argyi juice, then adding a decolorizing agent into the folium artemisiae argyi juice, stirring for 7min, and sieving with the 90-mesh sieve to obtain the folium artemisiae argyi juice;
the preparation process of the sow thistle juice comprises the following steps: firstly, selecting fresh and undamaged sow thistle leaves, washing the fresh and undamaged sow thistle leaves for 2 times by using deionized water, then putting the washed sow thistle leaves into a crushing device, crushing the sow thistle leaves for 6min at the rotating speed of 1800r/min, then sieving the crushed sow thistle leaves by using a 160-mesh sieve to obtain filtrate, then adding a decolorizing agent into the filtrate, stirring the filtrate for 10min, and then sieving the filtrate by using a 90-mesh sieve again to obtain sow thistle juice;
the preparation process of the garlic juice comprises the following steps: firstly, selecting full and peeled garlic, then putting the garlic into a crushing device, simultaneously adding 1.8 times of deionized water into the crushing device, then crushing for 12min at the rotating speed of 1800r/min, and sieving the crushed garlic with a 130-mesh sieve to obtain garlic juice;
the preparation process of the onion juice comprises the following steps: selecting peeled onion, cutting the onion into small particles, putting the small-particle onion into a crushing device, adding deionized water 2 times of the onion into the crushing device, crushing for 13min at the rotating speed of 1300r/min, and sieving the crushed onion with a 130-mesh sieve to obtain onion juice;
the preparation process of the bitter ginseng juice comprises the following steps: firstly, putting the sophora flavescens tablets into a crushing device, crushing for 15min at the rotating speed of 1800r/min to obtain sophora flavescens powder, then adding 2 times of deionized water into the sophora flavescens powder, uniformly stirring, and then sieving by a 100-mesh sieve to obtain sophora flavescens juice;
the preparation process of the lemon juice comprises the following steps: selecting fresh lemons, cleaning the fresh lemons with deionized water, slicing the lemons, removing seeds, putting the lemon slices into a container, and extruding the lemon slices for 13min under the pressure of 13MPa to obtain lemon juice.
The preparation method of the disinfectant for pathogenic microorganism experiments comprises the following steps:
s1, sequentially and respectively adding the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice into a container according to the weight percentage;
s2, stirring the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice in the container uniformly by using a stirring device;
s3, adding lemon juice into the uniformly stirred aloe juice, folium artemisiae argyi juice, sow thistle juice, garlic juice, onion juice and bitter ginseng juice according to the weight percentage, and uniformly stirring by using a stirring device again to obtain a mixture;
s4, packaging and quality testing the mixture obtained in the step S3 to obtain the disinfectant.
Example 3
The components of the disinfectant for pathogenic microorganism experiments comprise the following components in percentage by weight:
35% of aloe juice, 19% of folium artemisiae argyi juice, 13% of sow thistle juice, 15% of garlic juice, 11% of onion juice, 16% of radix sophorae flavescentis juice and 20% of lemon juice.
The preparation process of the aloe juice comprises the following steps: firstly, selecting full and undamaged aloe leaves, washing the aloe leaves for 3 times by using deionized water, cutting the cleaned aloe leaves into small blocks with uniform size, putting the small blocks of the aloe leaves into a crushing device, crushing the small blocks of the aloe leaves for 10min at the rotating speed of 2000r/min to obtain aloe crude juice, then sieving the obtained aloe crude juice with a 20-mesh sieve, putting the filtered aloe crude juice into a centrifugal device, and centrifuging the filtered aloe crude juice for 15min to obtain the aloe juice;
the preparation process of the folium artemisiae argyi juice comprises the following steps: firstly, selecting fresh and undamaged folium artemisiae argyi, cleaning the fresh and undamaged folium artemisiae argyi for 3 times by using deionized water, then putting the cleaned folium artemisiae argyi into a container, extruding the folium artemisiae argyi in the container under the pressure of 15MPa, taking out the folium artemisiae argyi in the container after the extrusion is finished, simultaneously sieving folium artemisiae argyi juice in the container by using a 120-mesh sieve, putting the extruded folium artemisiae argyi into the container again, extruding the folium artemisiae argyi again under the pressure of 8MPa, then taking out the folium artemisiae argyi from the container, sieving the folium artemisiae argyi juice in the container by using the 120-mesh sieve, uniformly mixing the folium artemisiae argyi juice after the two times of filtration, simultaneously adding 2 times of deionized water into the folium artemisiae argyi juice, then adding a decolorizing agent into the folium artemisiae argyi juice, stirring for 8min, and sieving by using the 100-mesh sieve to obtain the folium artemisiae argyi juice;
the preparation process of the sow thistle juice comprises the following steps: firstly, selecting fresh and undamaged sow thistle leaves, cleaning the fresh and undamaged sow thistle leaves for 3 times by using deionized water, then putting the cleaned sow thistle leaves into a crushing device, crushing the sow thistle leaves for 7min at the rotating speed of 2000r/min, then sieving the crushed sow thistle leaves by using a 200-mesh sieve to obtain filtrate, then adding a decolorizing agent into the filtrate, stirring for 10min, and then sieving by using a 100-mesh sieve again to obtain sow thistle juice;
the preparation process of the garlic juice comprises the following steps: firstly, selecting full and peeled garlic, then putting the garlic into a crushing device, simultaneously adding 2 times of deionized water into the crushing device, then crushing for 15min at the rotating speed of 2000r/min, and sieving the crushed garlic with a 150-mesh sieve to obtain garlic juice;
the preparation process of the onion juice comprises the following steps: selecting peeled onion, cutting the onion into small particles, putting the small-particle onion into a crushing device, adding deionized water which is 3 times of the onion into the crushing device, crushing for 15min at the rotating speed of 1500r/min, and sieving the crushed onion with a 150-mesh sieve to obtain onion juice;
the preparation process of the bitter ginseng juice comprises the following steps: firstly, putting the sophora flavescens tablets into a crushing device, crushing for 20min at the rotating speed of 2000r/min to obtain sophora flavescens powder, then adding 2 times of deionized water into the sophora flavescens powder, uniformly stirring, and then sieving by a 120-mesh sieve to obtain sophora flavescens juice;
the preparation process of the lemon juice comprises the following steps: selecting fresh lemons, cleaning the fresh lemons with deionized water, slicing the lemons, removing seeds, putting the lemon slices into a container, and extruding the lemon slices for 15min under the pressure of 15MPa to obtain lemon juice.
The preparation method of the disinfectant for pathogenic microorganism experiments comprises the following steps:
s1, sequentially and respectively adding the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice into a container according to the weight percentage;
s2, stirring the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice in the container uniformly by using a stirring device;
s3, adding lemon juice into the uniformly stirred aloe juice, folium artemisiae argyi juice, sow thistle juice, garlic juice, onion juice and bitter ginseng juice according to the weight percentage, and uniformly stirring by using a stirring device again to obtain a mixture;
s4, packaging and quality testing the mixture obtained in the step S3 to obtain the disinfectant.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. The components of the disinfectant for pathogenic microorganism experiments are characterized in that: comprises the following components in percentage by weight:
20-35% of aloe, 15-19% of folium artemisiae argyi, 10-13% of sow thistle, 8-15% of garlic, 7-11% of onion, 11-16% of radix sophorae flavescentis and 15-20% of lemon.
2. The disinfectant liquid for pathogenic microorganism experiments as set forth in claim 1, wherein the components comprise: comprises the following components in percentage by weight: 28% of aloe, 17% of folium artemisiae argyi, 10% of sow thistle, 11% of garlic, 8% of onion, 12% of radix sophorae flavescentis and 17% of lemon.
3. The disinfectant liquid for pathogenic microorganism experiments as set forth in claim 1, wherein the components comprise: the aloe, folium Artemisiae Argyi, herba Sonchi Oleracei, Bulbus Allii Cepae, radix Sophorae Flavescentis, and fructus Citri Limoniae are respectively aloe juice, folium Artemisiae Argyi juice, herba Sonchi Oleracei juice, Bulbus Allii Cepae juice, radix Sophorae Flavescentis juice, and fructus Citri Limoniae juice.
4. The disinfectant liquid for pathogenic microorganism experiments as set forth in claim 3, wherein: the preparation process of the aloe juice comprises the following steps: firstly, selecting full and undamaged aloe leaves, washing the aloe leaves for 2-3 times by using deionized water, cutting the cleaned aloe leaves into small blocks with uniform size, putting the small blocks of the aloe leaves into a crushing device, crushing the small blocks of the aloe leaves for 8-10 min at the rotating speed of 1500-2000 r/min to obtain aloe crude juice, sieving the obtained aloe crude juice by using a 10-20 mesh sieve, putting the filtered aloe crude juice into a centrifugal device, and centrifuging the filtered aloe crude juice for 10-15 min to obtain the aloe juice;
the preparation process of the folium artemisiae argyi juice comprises the following steps: firstly, selecting fresh and undamaged folium artemisiae argyi, cleaning the fresh and undamaged folium artemisiae argyi for 2-3 times by using deionized water, then putting the cleaned folium artemisiae argyi into a container, extruding the folium artemisiae argyi in the container under the pressure of 10-15 MPa, taking out the folium artemisiae argyi in the container after the extrusion is finished, simultaneously sieving the folium artemisiae argyi juice in the container by a sieve of 100-fold and 120-fold, putting the extruded folium artemisiae argyi into the container again, extruding the folium artemisiae argyi again under the pressure of 5-8 MPa, then taking out the folium artemisiae argyi from the container, sieving the folium artemisiae argyi juice in the container by a sieve of 100-fold and 120-fold, uniformly mixing the folium artemisiae argyi juice after the two times of filtration, simultaneously adding 1-2 times of deionized water into the folium artemisiae argyi juice, then adding a decolorizing agent into the folium artemisiae argyi juice, stirring for 5-8 min, and sieving the folium artemisiae argyi juice by a sieve of 80-100-fold to obtain the folium artemisiae argyi juice;
the preparation process of the sow thistle juice comprises the following steps: firstly, selecting fresh and undamaged sow thistle leaves, cleaning the fresh and undamaged sow thistle leaves for 2-3 times by using deionized water, then putting the cleaned sow thistle leaves into a crushing device, crushing the cleaned sow thistle leaves for 5-7 min at the rotating speed of 1500-2000 r/min, then sieving the crushed sow thistle leaves with a 100-mesh sieve and 200-mesh sieve to obtain filtrate, then adding a decolorizing agent into the filtrate, stirring for 10min, and then sieving with a 80-100-mesh sieve again to obtain sow thistle juice;
the preparation process of the garlic juice comprises the following steps: firstly, selecting full and peeled garlic, then putting the garlic into a crushing device, simultaneously adding 1.5-2 times of deionized water into the crushing device, then crushing for 10-15 min at the rotating speed of 1500-2000 r/min, and sieving the crushed garlic with a 120-fold 150-mesh sieve to obtain garlic juice;
the preparation process of the onion juice comprises the following steps: selecting peeled onion, cutting the onion into small particles, putting the small-particle onion into a crushing device, adding deionized water 2-3 times of the onion into the crushing device, crushing for 10-15 min at the rotating speed of 1000-1500 r/min, and sieving the crushed onion with a sieve of 120-150 meshes to obtain onion juice;
the preparation process of the bitter ginseng juice comprises the following steps: firstly, putting the sophora flavescens tablets into a crushing device, crushing for 10min-20min at the rotating speed of 1500r/min-2000r/min to obtain sophora flavescens powder, then adding 2 times of deionized water into the sophora flavescens powder, uniformly stirring, and then sieving by a sieve of 80-120 meshes to obtain sophora flavescens juice;
the preparation process of the lemon juice comprises the following steps: selecting fresh lemons, cleaning the fresh lemons with deionized water, slicing the lemons, removing seeds, putting the lemon slices into a container, and extruding the lemon slices for 10-15 min under the pressure of 10-15 MPa to obtain lemon juice.
5. The preparation method of the disinfectant for pathogenic microorganism experiments is characterized by comprising the following steps: the method comprises the following steps:
s1, sequentially and respectively adding the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice into a container according to the weight percentage;
s2, stirring the aloe juice, the folium artemisiae argyi juice, the sow thistle juice, the garlic juice, the onion juice and the sophora flavescens juice in the container uniformly by using a stirring device;
s3, adding lemon juice into the uniformly stirred aloe juice, folium artemisiae argyi juice, sow thistle juice, garlic juice, onion juice and bitter ginseng juice according to the weight percentage, and uniformly stirring by using a stirring device again to obtain a mixture;
s4, packaging and quality testing the mixture obtained in the step S3 to obtain the disinfectant.
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