CN113942216A - Production method of high-capacity cell shake flask - Google Patents
Production method of high-capacity cell shake flask Download PDFInfo
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- CN113942216A CN113942216A CN202111207086.1A CN202111207086A CN113942216A CN 113942216 A CN113942216 A CN 113942216A CN 202111207086 A CN202111207086 A CN 202111207086A CN 113942216 A CN113942216 A CN 113942216A
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 44
- 238000000071 blow moulding Methods 0.000 claims abstract description 47
- 238000010438 heat treatment Methods 0.000 claims abstract description 37
- 238000001746 injection moulding Methods 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000007599 discharging Methods 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 7
- 229920005644 polyethylene terephthalate glycol copolymer Polymers 0.000 claims description 5
- 238000007493 shaping process Methods 0.000 claims description 4
- 230000007704 transition Effects 0.000 claims description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 239000004033 plastic Substances 0.000 abstract description 3
- 229920003023 plastic Polymers 0.000 abstract description 3
- 239000005020 polyethylene terephthalate Substances 0.000 description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 238000007664 blowing Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- -1 polyethylene terephthalate Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/02—Combined blow-moulding and manufacture of the preform or the parison
- B29C49/06—Injection blow-moulding
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/28—Blow-moulding apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/42—Component parts, details or accessories; Auxiliary operations
- B29C49/4242—Means for deforming the parison prior to the blowing operation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/42—Component parts, details or accessories; Auxiliary operations
- B29C49/48—Moulds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/42—Component parts, details or accessories; Auxiliary operations
- B29C49/48—Moulds
- B29C49/4823—Moulds with incorporated heating or cooling means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/42—Component parts, details or accessories; Auxiliary operations
- B29C49/64—Heating or cooling preforms, parisons or blown articles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/42—Component parts, details or accessories; Auxiliary operations
- B29C49/78—Measuring, controlling or regulating
- B29C49/786—Temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/02—Combined blow-moulding and manufacture of the preform or the parison
- B29C2049/023—Combined blow-moulding and manufacture of the preform or the parison using inherent heat of the preform, i.e. 1 step blow moulding
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C49/00—Blow-moulding, i.e. blowing a preform or parison to a desired shape within a mould; Apparatus therefor
- B29C49/42—Component parts, details or accessories; Auxiliary operations
- B29C49/48—Moulds
- B29C2049/4879—Moulds characterised by mould configurations
- B29C2049/4882—Mould cavity geometry
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Abstract
The invention discloses a production method of a large-capacity cell shake flask, which belongs to the technical field of plastic product production and solves the production problem of the large-capacity cell shake flask, and the production method utilizes full-automatic integrated blow molding equipment with an injection molding device, an injection molding unit, a heating unit, a blow molding unit, a discharging unit and a conveying unit to produce the large-capacity cell shake flask; the invention connects each procedure of the process method closely, has raised the production efficiency; the invention optimizes the sectional heating parameters in the heating procedure, solves the one-step forming problem of the large-capacity shake flask by the blow molding die in the blow molding procedure, is beneficial to the stable forming of the large-capacity shake flask, and ensures the stable performance of the finished shake flask.
Description
Technical Field
The invention belongs to the technical field of plastic product production, and relates to a production method of a high-capacity cell shake flask.
Background
In the market, the specifications of the cell shake flasks are mostly small-capacity products with the volume less than 1L, such as 125mL, 250mL, 500mL and 1000mL, and the small-capacity cell shake flasks can be better suitable for experimental culture or pilot-scale cell culture and are not suitable for cell culture in production, so that production enterprises need large-capacity cell shake flask products to improve the productivity, and then large-capacity cell shake flasks are needed, and the market cannot meet the requirements of the large-capacity cell shake flasks at present.
The existing shake flask is often formed by adopting an extrusion-blow process, redundant clamp glue needs to be removed by mechanical or manual pinning after the bottle adopting the extrusion-blow process is demoulded, the bottle mouth also needs to be polished and trimmed, and a joint line clamp mouth also exists at the bottle bottom, so that the plastic shake flask loses stability, the processing technology is complex, the working procedures are multiple, and manual polishing and trimming need to be consumed.
Although patent No. 201080059124.7 discloses a preform for a large container, which is used for a large container made of polyethylene terephthalate (hereinafter, also referred to as PET) resin and having a capacity of 10 liters or more, a large container, and a method for producing a large container, the preform is not suitable for cell shake flask production; there are also a blow molding method, a blow mold and a blow molding apparatus of patent No. 201980031157.1, in which a blow molding apparatus including an injection unit, a temperature adjusting unit, a take-out unit and a transfer device is disclosed, but a production method and a production mold of a cell flask are not disclosed.
Disclosure of Invention
The invention aims to solve the problem of production of a large-capacity cell shake flask, provides a production mold of the large-capacity cell shake flask, and provides a production method of the large-capacity cell shake flask in a matching way.
The technical scheme adopted by the invention is as follows:
a production method of a large-capacity cell shake flask is used for production by full-automatic integrated blow molding equipment with an injection molding device, an injection molding unit, a heating unit, a blow molding unit, a discharging unit and a conveying unit, and comprises the following process steps:
step one, an injection molding process, namely injecting production raw materials into an injection molding unit by an injection molding device to form a bottle blank, wherein the bottle blank is of a cylindrical blank body structure with an arc convex bottom, and an opening of the bottle blank enters a conveying unit and is extruded and molded by a conveying mold to form a bottle opening with external threads;
the conveying mould is formed by combining two half moulds, and the conveying mould can be opened and closed, namely the first half conveying mould and the second half conveying mould are combined to form the conveying mould, and the first half conveying mould and the second half conveying mould can be driven by a mechanical device in the conveying unit to complete opening and closing actions;
step two, a heating procedure, namely moving the bottle blank from the injection molding unit to a heating unit through a conveying unit, and heating the bottle blank by the heating unit in a segmented manner;
a third step of performing blow molding, namely moving the heated bottle blank from the heat unit to a blow molding unit through a conveying unit, performing blow molding on the bottle blank by using a blow molding die, and cooling and fixing the shape to obtain a molded bottle body;
and step four, an ejection process, namely moving the molded bottle bodies from the blow molding unit to an ejection unit through the conveying unit, then opening a first half conveying mold and a second half conveying mold in the conveying unit, and then pushing the bottle bodies out of the full-automatic integrated blow molding equipment through the ejection unit.
Further, the first half conveying die and the second half conveying die in the first step are identical in structure and respectively comprise a hollow circular truncated cone arranged on the opening plate, the hollow portion of the hollow circular truncated cone penetrates through the opening plate, and an internal thread is arranged on the inner wall of the lower end portion of the hollow circular truncated cone.
Further, the step two comprises the step of heating in a segmented manner, wherein a PETG material is used as a production raw material, and the specific parameters are as follows: the heating temperature of the bottle mouth is 240-250 ℃, the heating temperature of the front section of the bottle blank is 230-240 ℃, the heating temperature of the middle section of the bottle blank is 220-230 ℃, and the heating temperature of the rear section of the bottle blank is 220-230 ℃.
Further, the blow mold in the third step includes a first half blow mold and a second half blow mold half-and-half arranged in half, and a column, wherein the first half blow mold and the second half blow mold can be opened and closed and used for blow molding of the bottle body, and the column is used for assisting in molding of the bottle bottom.
Specifically, the first half blow molding die and the second half blow molding die are identical in structure and both consist of a cooling jacket and a section die, wherein the concave area of the section die comprises a conveying die concave arc for accommodating a conveying die, a bottle body concave arc for shaping a bottle body and an upright post concave arc for accommodating an upright post; and the four corners of the section mould also comprise tool holes for tools.
The large-capacity cell shake flask produced by the blow molding die comprises a flask opening, a ring bulge, a flask neck, a flask shoulder and a flask bottom, wherein the flask opening is provided with an external thread, the ring bulge is an arc bulge girdle band between the flask opening and the flask neck, the flask neck is cylindrical, the flask shoulder is naturally formed by downward transition of the flask neck, a flask body is expanded below the flask shoulder, and the flask body is smoothly transited to the flask bottom to form the arc flask bottom.
Preferably, the size of a large-capacity cell shake flask is 2.8L, the overall height is 245mm, the diameter of the bottle neck is 70mm, and the diameter of the bottle bottom is 163 mm.
Preferably, the size of the large-capacity cell shake flask is 5L, the whole height is 281mm, the diameter of the bottle neck is 90mm, and the diameter of the bottle bottom is 230 mm.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the production equipment, the production raw materials and the production method adopted by the invention can ensure reproducible mass production of finished product cell shake flasks, and solve the problem of market shortage of large-capacity shake flasks.
2. The production method utilizes full-automatic integrated blow molding equipment, simplifies the process method into an injection molding process, a heating process, a blow molding process and a discharging process, and improves the production efficiency because the processes are closely connected.
3. The invention optimizes the sectional heating parameters in the heating process, solves the problem of high-capacity shake flask forming by the blow mold in the blow molding process, is beneficial to the high-capacity shake flask forming stability, reduces the generation probability of defective products and ensures the stable performance of the finished shake flask.
4. The large-capacity shake flask produced by the invention has the specification of 2.8L or 5L, the bottle mouth area is larger than that of similar products, the ventilation capacity is larger, the shake flask is suitable for high-density culture of cells, the working volume can be 60-80% of the total volume, and the cell yield is higher.
Drawings
FIG. 1 is a schematic view of the entire production apparatus of the present invention;
FIG. 2 is a schematic view of the apparatus for the blow molding step of the present invention;
FIG. 3 is a schematic view of the blow mold of the present invention;
FIG. 4 is a schematic view of the structure of the transfer mold of the present invention;
FIG. 5 is a schematic view of the structure of the bottle preform according to the present invention;
FIG. 6 is a schematic diagram of the structure of the finished shake flask of the cell produced by the present invention;
the labels in the figure are: 1-bottle mouth, 2-ring bulge, 3-bottle neck, 4-bottle shoulder, 5-bottle bottom, 6-bottle blank, 10-injection molding device, 20-injection molding unit, 30-heating unit, 40-blow molding unit, 50-discharging unit, 60-conveying unit, 601-first half conveying mold, 602-second half conveying mold, 6011-hollow circular table, 6012-split plate, 6013-internal thread, 401-upright post, 402-first half blow molding mold, 403-second half blow molding mold, 4021-middle partition, 4022-conveying mold concave arc, 4023-bottle body concave arc, 4024-upright post concave arc and 4025-tool hole.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.
As shown in FIG. 1, a method for producing a large-capacity cell shake flask, which utilizes a fully automatic integrated blow molding equipment ASB-650EXHS, according to the following steps, the large-capacity cell shake flask shown in FIG. 6 is produced.
The production raw materials of the large-capacity cell shake flask are easy to be formed by injection, drawing and blowing, and the melt flow rate is 1.0-10.0 g/10min, preferably 3.0-5.0 g/10 min.
Specifically, the production raw material can be selected from PETG material, PC material, COP material, COC material, CBC material and PET material.
A production method of a large-capacity cell shake flask selects a PETG material as a production raw material, and specifically comprises the following steps:
step one, an injection molding process, in which the injection molding device 10 injects raw materials into the injection molding unit 20 to form a cylindrical bottle blank 6 with an arc convex bottom, as shown in fig. 5, wherein an opening of the bottle blank enters the conveying unit 60 and is extruded and molded by a conveying mold in the conveying unit 60 to form the bottle mouth 1 with external threads.
The conveying mold is formed by combining two half molds, and the conveying mold can be opened and closed, namely the first half conveying mold 601 and the second half conveying mold 602 are combined to form the conveying mold, and the first half conveying mold 601 and the second half conveying mold 602 can be driven by a mechanical device in the conveying unit 60 to complete opening and closing actions; as shown in fig. 4, the first half conveyance die 601 is exemplified as a structural feature, and the first half conveyance die 601 includes a hollow circular truncated cone 6011 provided on an opening plate 6012, the hollow portion of the hollow circular truncated cone 6011 penetrates through the opening plate, and an internal thread 6013 is provided on an inner wall of a lower end portion of the hollow circular truncated cone 6011.
In order to improve the blow molding efficiency, it is preferable that the first half transfer mold 601 has two hollow circular truncated cones, and two bottle blanks can be simultaneously injected and transported.
And step two, a heating procedure, namely moving the bottle blank 6 from the injection molding unit 20 to the heating unit 30 through the conveying unit 60, and heating the bottle blank by the heating unit in a segmented manner.
According to the characteristics of the PETG material as the raw material for production, the parameters for heating the bottle blank 6 in sections are as follows: the heating temperature of the bottle mouth is 240-250 ℃, the heating temperature of the front section of the bottle blank is 230-240 ℃, the heating temperature of the middle section of the bottle blank is 220-230 ℃, and the heating temperature of the rear section of the bottle blank is 220-230 ℃; the sectional heating can obtain better physical properties of the material and is beneficial to the subsequent blow molding process.
A third step of performing a blow molding process of moving the heated bottle blank from the heat unit 30 to the blow molding unit 40 through the conveying unit 60, wherein the blow mold in the blow molding unit 40 comprises a first half blow mold 402 and a second half blow mold 403 which are arranged in half, and a column 401, as shown in fig. 2; the first half blow mold 402 and the second half blow mold 403 can be opened and closed and used for blow molding of the bottle body, and the upright column 401 is used for assisting in the bottom molding; and carrying out blow molding on the bottle blank by using a blow molding die, and cooling and fixing the shape to obtain a molded bottle body.
As shown in fig. 3, the structural features for body shaping are specifically described by taking a first half blow mold 402 as an example, the first half blow mold 402 is composed of a cooling jacket and a forming mold, wherein the recessed area of the forming mold comprises a conveying mold concave arc 4022 for accommodating a first half conveying mold 601 and a second half conveying mold 602, a body concave arc 4023 for body shaping, and a column concave arc 4024 for accommodating a column 401; four corners of the pattern die further comprise tooling holes 4025 for tooling.
In order to improve the production efficiency, the first half blow molding die 402 is arranged by matching with two hollow circular truncated cones on the first half conveying die 601, preferably two section dies are arranged, the structure of each section die is the same, and a concave area of each section die is provided with a die concave arc, a bottle body concave arc and an upright post concave arc; an intermediate partition is arranged between the two moulds and is fixedly arranged in the cooling jacket.
And step four, an ejection process, namely moving the molded bottle bodies from the blow molding unit 40 to the ejection unit 50 through the conveying unit 60, then opening the first half conveying mold 601 and the second half conveying mold 602 in the conveying unit 60, and then pushing the bottle bodies out of the full-automatic integrated blow molding equipment through the ejection unit 50.
According to the production method of the large-capacity cell shake flask, the produced product is the large-capacity cell shake flask, as shown in fig. 6, the product comprises a flask opening 1, a ring protrusion 2, a flask neck 3, a flask shoulder 4 and a flask bottom 5, wherein the flask opening 1 is provided with an external thread for connecting a flask cover, the ring protrusion 2 is an arc convex ring belt positioned between the flask opening 1 and the flask neck 3 and used for limiting a matched handle, so that the handle is stably sleeved on the cell shake flask to facilitate taking and transferring of the shake flask, the flask neck 3 is regular cylindrical and naturally forms the flask shoulder 4 in downward transition, the lower part of the flask shoulder 4 is expanded into a flask body, and the flask body is smoothly transited to the flask bottom 5 to form an arc flask bottom 5.
The specific capacity of the shake flask is preferably 2.8L or 5L, compared with the shake flask with the volume of less than 1L circulated in the market, the shake flask has larger specification, larger bottleneck area than similar products and larger ventilation capacity, is suitable for high-density culture of cells, the working volume can be 60-80% of the total volume, and the cell yield is higher.
Preferably, the 2.8L large-capacity cell shake flask has the overall height of 245mm, the diameter of the bottle neck of 70mm and the diameter of the bottle bottom of 163 mm; the culture volume of the shake flask of the cells with the specification is 1.6L-2.2L.
Preferably, the whole height of the 5L-sized large-capacity cell shake flask is 281mm, the diameter of a bottle neck is 90mm, and the diameter of a bottle bottom is 230 mm; the culture volume of the shake flask of the cells with the specification is 3L-4L.
Claims (8)
1. A production method of a large-capacity cell shake flask is characterized by utilizing full-automatic integrated blow molding equipment with an injection molding device, an injection molding unit, a heating unit, a blow molding unit, a discharging unit and a conveying unit to produce, and comprising the following process steps:
step one, an injection molding process, namely injecting production raw materials into an injection molding unit by an injection molding device to form a bottle blank, wherein the bottle blank is of a cylindrical blank body structure with an arc convex bottom, and an opening of the bottle blank enters a conveying unit and is extruded and molded by a conveying mold to form a bottle opening with external threads;
the conveying mould is formed by combining two half moulds, and the conveying mould can be opened and closed, namely the first half conveying mould and the second half conveying mould are combined to form the conveying mould, and the first half conveying mould and the second half conveying mould can be driven by a mechanical device in the conveying unit to complete opening and closing actions;
step two, a heating procedure, namely moving the bottle blank from the injection molding unit to a heating unit through a conveying unit, and heating the bottle blank by the heating unit in a segmented manner;
a third step of performing blow molding, namely moving the heated bottle blank from the heat unit to a blow molding unit through a conveying unit, performing blow molding on the bottle blank by using a blow molding die, and cooling and fixing the shape to obtain a molded bottle body;
and step four, an ejection process, namely moving the molded bottle bodies from the blow molding unit to an ejection unit through the conveying unit, then opening a first half conveying mold and a second half conveying mold in the conveying unit, and then pushing the bottle bodies out of the full-automatic integrated blow molding equipment through the ejection unit.
2. A production method of a large-capacity cell shake flask according to claim 1, wherein the first half conveying mold and the second half conveying mold in the first step have the same structure, and both comprise a hollow circular truncated cone arranged on the open-close plate, the hollow part of the hollow circular truncated cone penetrates through the open-close plate, and the inner wall of the lower end part of the hollow circular truncated cone is provided with an internal thread.
3. A production method of a large-capacity cell shake flask according to claim 1, wherein the step two of heating in stages uses PETG material as raw material, and the specific parameters are as follows: the heating temperature of the bottle mouth is 240-250 ℃, the heating temperature of the front section of the bottle blank is 230-240 ℃, the heating temperature of the middle section of the bottle blank is 220-230 ℃, and the heating temperature of the rear section of the bottle blank is 220-230 ℃.
4. A method for producing a large capacity cell shake flask according to claim 1, wherein the blow mold in the third step comprises a first half blow mold and a second half blow mold arranged in half, and a pillar, wherein the first half blow mold and the second half blow mold can be opened and closed and used for blow molding of a bottle body, and wherein the pillar is used for assisting the bottom of the bottle to be shaped.
5. A large capacity cell shake flask production method according to claim 4, wherein the first half blow mold and the second half blow mold are identical in structure and each composed of a cooling jacket and a mold, wherein the recessed area of the mold comprises a conveying mold concave arc for accommodating the conveying mold, a body concave arc for shaping the body, and a column concave arc for accommodating the column; and the four corners of the section mould also comprise tool holes for tools.
6. The method for producing a large-capacity cell shake flask according to any one of claims 1 to 5, wherein the large-capacity cell shake flask produced by using the blow mold comprises a flask opening, a ring bulge, a flask neck, a flask shoulder and a flask bottom, wherein the flask opening is provided with an external thread, the ring bulge is an arc bulge girdle ring positioned between the flask opening and the flask neck, the flask neck is cylindrical, the downward transition of the flask neck naturally forms the flask shoulder, the flask body is expanded below the flask shoulder, and the flask body and the flask bottom smoothly transition form the arc flask bottom.
7. A large capacity cell shake flask according to claim 6, wherein a large capacity cell shake flask is 2.8L in size, 245mm in overall height, 70mm in diameter of the neck and 163mm in diameter of the bottom.
8. A large capacity cell shake flask according to claim 6, wherein a large capacity cell shake flask is 5L in size, having an overall height of 281mm, a neck diameter of 90mm and a bottom diameter of 230 mm.
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| CN202111207086.1A CN113942216A (en) | 2021-10-15 | 2021-10-15 | Production method of high-capacity cell shake flask |
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| CN202111207086.1A CN113942216A (en) | 2021-10-15 | 2021-10-15 | Production method of high-capacity cell shake flask |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE824127A (en) * | 1974-01-09 | 1975-07-07 | IMPROVEMENTS IN THE MANUFACTURING OF HOLLOW CONTAINERS IN THERMOPLASTIC MATERIAL ORIENTED AXIALLY IN BOTH DIRECTIONS | |
| US4717668A (en) * | 1986-12-12 | 1988-01-05 | Baxter Travenol Laboratories, Inc. | Plastic roller bottle for suspension cultures |
| DE19917916A1 (en) * | 1999-04-20 | 2000-10-26 | Dalli Werke Waesche & Koerperp | Plastic bottle manufacturing process involves injecting a PET preform with varying wall thickness, orienting the preform and locating in a non-symmetric blow mold for bottle blowing |
| CN2432037Y (en) * | 2000-06-29 | 2001-05-30 | 凯美塑胶机械股份有限公司 | Plastic blank holding positioning and blow-forming equipment for hollow moulding machine |
| GB0511732D0 (en) * | 2004-06-09 | 2005-07-20 | Scient Plastic Products Inc | Fermentation flask for cultivating microorganisms, a growth media and a method of use |
| CN207468646U (en) * | 2017-08-16 | 2018-06-08 | 苏州米迪生物技术有限公司 | High efficiency cell culture bottle |
| CN208290455U (en) * | 2018-04-20 | 2018-12-28 | 台州市黄岩业康机械有限公司 | PET plastic bottle blowing machine high-precision heating system |
| CN208395180U (en) * | 2018-05-25 | 2019-01-18 | 苏州米迪生物技术有限公司 | Pre-install the disposable shaking flask of microcarrier |
| CN209111503U (en) * | 2018-11-08 | 2019-07-16 | 广州紫江包装有限公司 | A kind of plastic bottle blank heater |
-
2021
- 2021-10-15 CN CN202111207086.1A patent/CN113942216A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE824127A (en) * | 1974-01-09 | 1975-07-07 | IMPROVEMENTS IN THE MANUFACTURING OF HOLLOW CONTAINERS IN THERMOPLASTIC MATERIAL ORIENTED AXIALLY IN BOTH DIRECTIONS | |
| US4717668A (en) * | 1986-12-12 | 1988-01-05 | Baxter Travenol Laboratories, Inc. | Plastic roller bottle for suspension cultures |
| DE19917916A1 (en) * | 1999-04-20 | 2000-10-26 | Dalli Werke Waesche & Koerperp | Plastic bottle manufacturing process involves injecting a PET preform with varying wall thickness, orienting the preform and locating in a non-symmetric blow mold for bottle blowing |
| CN2432037Y (en) * | 2000-06-29 | 2001-05-30 | 凯美塑胶机械股份有限公司 | Plastic blank holding positioning and blow-forming equipment for hollow moulding machine |
| GB0511732D0 (en) * | 2004-06-09 | 2005-07-20 | Scient Plastic Products Inc | Fermentation flask for cultivating microorganisms, a growth media and a method of use |
| CN207468646U (en) * | 2017-08-16 | 2018-06-08 | 苏州米迪生物技术有限公司 | High efficiency cell culture bottle |
| CN208290455U (en) * | 2018-04-20 | 2018-12-28 | 台州市黄岩业康机械有限公司 | PET plastic bottle blowing machine high-precision heating system |
| CN208395180U (en) * | 2018-05-25 | 2019-01-18 | 苏州米迪生物技术有限公司 | Pre-install the disposable shaking flask of microcarrier |
| CN209111503U (en) * | 2018-11-08 | 2019-07-16 | 广州紫江包装有限公司 | A kind of plastic bottle blank heater |
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Application publication date: 20220118 |