CN113940895A - 一种抗菌肽洗手液及其制备方法 - Google Patents
一种抗菌肽洗手液及其制备方法 Download PDFInfo
- Publication number
- CN113940895A CN113940895A CN202010680313.1A CN202010680313A CN113940895A CN 113940895 A CN113940895 A CN 113940895A CN 202010680313 A CN202010680313 A CN 202010680313A CN 113940895 A CN113940895 A CN 113940895A
- Authority
- CN
- China
- Prior art keywords
- antibacterial peptide
- hand sanitizer
- deionized water
- humectant
- emulsifier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003910 polypeptide antibiotic agent Substances 0.000 title claims abstract description 49
- 238000002360 preparation method Methods 0.000 title abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 13
- 239000003906 humectant Substances 0.000 claims abstract description 13
- 239000004094 surface-active agent Substances 0.000 claims abstract description 13
- 239000008367 deionised water Substances 0.000 claims abstract description 12
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- 101000741302 Bungarus fasciatus Cathelicidin-related antimicrobial peptide Bf-CRAMP Proteins 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 6
- FPVVYTCTZKCSOJ-UHFFFAOYSA-N Ethylene glycol distearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCCOC(=O)CCCCCCCCCCCCCCCCC FPVVYTCTZKCSOJ-UHFFFAOYSA-N 0.000 claims description 5
- MRUAUOIMASANKQ-UHFFFAOYSA-N cocamidopropyl betaine Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O MRUAUOIMASANKQ-UHFFFAOYSA-N 0.000 claims description 5
- 235000011187 glycerol Nutrition 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 4
- -1 methyl taurate Chemical compound 0.000 claims description 4
- 229940057950 sodium laureth sulfate Drugs 0.000 claims description 4
- SXHLENDCVBIJFO-UHFFFAOYSA-M sodium;2-[2-(2-dodecoxyethoxy)ethoxy]ethyl sulfate Chemical compound [Na+].CCCCCCCCCCCCOCCOCCOCCOS([O-])(=O)=O SXHLENDCVBIJFO-UHFFFAOYSA-M 0.000 claims description 4
- 229940073507 cocamidopropyl betaine Drugs 0.000 claims description 3
- SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 229940100460 peg-100 stearate Drugs 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 2
- 229940043375 1,5-pentanediol Drugs 0.000 claims description 2
- 239000004475 Arginine Substances 0.000 claims description 2
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 2
- 239000004386 Erythritol Substances 0.000 claims description 2
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
- ALQSHHUCVQOPAS-UHFFFAOYSA-N Pentane-1,5-diol Chemical compound OCCCCCO ALQSHHUCVQOPAS-UHFFFAOYSA-N 0.000 claims description 2
- 229920002385 Sodium hyaluronate Polymers 0.000 claims description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 235000009697 arginine Nutrition 0.000 claims description 2
- 229940081733 cetearyl alcohol Drugs 0.000 claims description 2
- 235000015165 citric acid Nutrition 0.000 claims description 2
- 229940113120 dipropylene glycol Drugs 0.000 claims description 2
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 2
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 claims description 2
- 229940009714 erythritol Drugs 0.000 claims description 2
- 235000019414 erythritol Nutrition 0.000 claims description 2
- 229930182478 glucoside Natural products 0.000 claims description 2
- 150000008131 glucosides Chemical class 0.000 claims description 2
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 claims description 2
- 229910000028 potassium bicarbonate Inorganic materials 0.000 claims description 2
- 235000015497 potassium bicarbonate Nutrition 0.000 claims description 2
- 239000011736 potassium bicarbonate Substances 0.000 claims description 2
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 claims description 2
- 229940083542 sodium Drugs 0.000 claims description 2
- 229940010747 sodium hyaluronate Drugs 0.000 claims description 2
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims description 2
- 229940104261 taurate Drugs 0.000 claims description 2
- 229940074410 trehalose Drugs 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims 1
- 239000003002 pH adjusting agent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 14
- 238000005406 washing Methods 0.000 abstract description 10
- 239000000126 substance Substances 0.000 abstract description 9
- 230000001954 sterilising effect Effects 0.000 abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 7
- 238000005202 decontamination Methods 0.000 abstract description 3
- 230000003588 decontaminative effect Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000005265 energy consumption Methods 0.000 abstract 1
- 230000000844 anti-bacterial effect Effects 0.000 description 13
- 238000012360 testing method Methods 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- 206010040880 Skin irritation Diseases 0.000 description 8
- 230000036556 skin irritation Effects 0.000 description 8
- 231100000475 skin irritation Toxicity 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 6
- 230000003385 bacteriostatic effect Effects 0.000 description 5
- 230000007794 irritation Effects 0.000 description 5
- 108700042778 Antimicrobial Peptides Proteins 0.000 description 4
- 102000044503 Antimicrobial Peptides Human genes 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 239000000344 soap Substances 0.000 description 4
- 206010012735 Diarrhoea Diseases 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 3
- 230000000845 anti-microbial effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000002147 killing effect Effects 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 241000270295 Serpentes Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000008233 hard water Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 208000004926 Bacterial Vaginosis Diseases 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 206010024769 Local reaction Diseases 0.000 description 1
- SUZRRICLUFMAQD-UHFFFAOYSA-N N-Methyltaurine Chemical compound CNCCS(O)(=O)=O SUZRRICLUFMAQD-UHFFFAOYSA-N 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 229940064004 antiseptic throat preparations Drugs 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229940121657 clinical drug Drugs 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 210000005007 innate immune system Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000036620 skin dryness Effects 0.000 description 1
- 239000003998 snake venom Substances 0.000 description 1
- 229940104256 sodium taurate Drugs 0.000 description 1
- GWLWWNLFFNJPDP-UHFFFAOYSA-M sodium;2-aminoethanesulfonate Chemical compound [Na+].NCCS([O-])(=O)=O GWLWWNLFFNJPDP-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
- A61K8/466—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfonic acid derivatives; Salts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/86—Polyethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Emergency Medicine (AREA)
- Dermatology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明涉及日化洗护产品技术领域，具体涉及一种抗菌肽洗手液及其制备方法。该洗手液包括以下按重量百分比的组分：抗菌肽0.2%‑5%、表面活性剂20%‑40%、保湿剂0.5%‑5%、乳化剂2%‑5%、pH调节剂0.05%‑0.8%以及去离子水45%‑77%。本发明制得的洗手液具有良好的去污性，杀菌抑菌效果佳，亲肤不伤手等性能，且制备工艺简单，能耗低。
Description
技术领域
本发明涉及日化洗护产品技术领域，具体涉及一种抗菌肽洗手液及其制备方法。
背景技术
据世界卫生组织(WHO)报道：目前腹泻和肺炎是导致儿童死亡的最主要元凶，全球每年有170万人死于腹泻类疾病，另有150万人死于下呼吸道疾病，绝大多数是发展中国家儿童。用肥皂洗手可以将腹泻致死率减半、急性呼吸道感染致死率减少三分之一。
传统的固体肥皂，虽然具有清洁和杀菌效果，但在使用时会粘附使用者手上的污垢、细菌，容易形成二次污染源，增加了细菌交叉感染的几率。而洗手液在取用时通过机械泵挤压，手的接触面仅限于泵头处，具有使用方便、避免二次污染等特点，所以近年来洗手液已经逐渐取代香皂。目前市场上销售的洗手液主要以清洁作用为主，大部分都不具有抑菌功能。具有抑菌功能的洗手液主要是通过添加化学杀菌剂来实现，这些化学物质大多具有生理毒性，对生态环境也会造成污染。一些无毒的杀菌剂，例如酒精等，因为会对皮肤造成刺激，长期使用会导致皮肤干燥、粗糙和皲裂。因此，开发不伤皮肤，无毒、无污染的生物抑菌类洗手液已是社会发展所需。而肽类物质对皮肤相对安全，利用活性多肽制备生物类抑菌洗涤用品具有重要的开发价值。
抗菌肽广泛分布于自然界的各种生物内，是先天免疫系统的一部分，是抵御外界病原微生物侵袭的第一道防线。抗菌肽具有广谱抗细菌、抗真菌、抗病毒、抗肿瘤的活性，而且具有不易产生耐药的特性。金环蛇抗菌肽Cathelicidin-BF是从金环蛇蛇毒中分离出来的活性多肽，是金环蛇基因编码的一种N-端有α-螺旋的直链多肽，含有三十个氨基酸残基，分子量3637.54Da。Cathelicidin-BF对革兰氏阳性菌和革兰氏阴性菌具有非常强的杀菌活性，作用时间迅速，几乎没有溶血活性和细胞毒性。有研究证实其可以在几分钟内杀死细菌，且不产生耐药性，而抗生素则至少需要3h，且易产生耐药性。目前，申请号为200810058976.9的发明专利公开了金环蛇抗菌肽Cathelicidin-BF及其基因和应用，公开了金环蛇抗菌肽Cathelicidin-BF分子量小、杀菌作用强、作用时间迅速、对多种临床耐药菌有非常强的杀灭作用，此外还具有广谱抗菌性和非盐依赖等有效特点；申请号为201110265011.9 的发明专利公开了金环蛇抗菌肽Cathelicidin-BF在制备治疗细菌性阴道炎药物中的作用。但是利用金环蛇抗菌肽Cathelicidin-BF制备洗手液及其制备方法未见报道。
发明内容
为解决现有技术存在的问题，本发明提供了一种以金环蛇抗菌肽 Cathelicidin-BF作为抑菌组分的抗菌肽洗手液及其制备方法。
本发明所采用的技术方案是：
一种抗菌肽洗手液，由抗菌肽、表面活性剂、保湿剂、乳化剂、 pH调节剂以及去离子水组成。
优选地，洗手液中成分的重量百分比为：抗菌肽0.2％-5％、表面活性剂20％-40％、保湿剂0.5％-5％、乳化剂2％-5％、pH调节剂 0.05％-0.8％以及去离子水45％-77％。
进一步优选，抗菌肽0.5％-2％、表面活性剂20％-30％、保湿剂 1％-3％、乳化剂2.5％-4％、pH调节剂0.1％-0.5％以及去离子水 61％-75％。
作为优选方案，抗菌肽是金环蛇抗菌肽Cathelicidin-BF。
优选地，表面活性剂选自月桂醇聚醚硫酸酯钠、椰油酰胺丙基甜菜碱、椰油酰甲基牛磺酸牛磺酸钠、椰油酰胺中的一种或多种。
优选地，保湿剂选自丁二醇、甘油、水解透明质酸钠、海藻糖、 1，5-戊二醇，赤藓糖醇，双丙甘醇中的一种或多种。
优选地，乳化剂选自鲸蜡硬脂基葡糖苷、鲸蜡硬脂醇、PEG-100 硬脂酸酯、乙二醇二硬脂酸酯中的一种或多种。
优选地，pH调节剂选自EDTA二钠、柠檬酸、碳酸氢钾、精氨酸中的一种或多种。
此外，本发明还提供一种抗菌肽洗手液的制备方法，包括以下步骤：
步骤1：取1/4水加入乳化剂组份加热，中速均质搅拌溶解；
步骤2：再取1/4水溶解表面活性剂组份，然后加入保湿剂组份以真空高速搅拌溶解；
步骤3：将步骤1和步骤2的溶液混合，加入用剩余水溶解好的pH 调节剂，维持中速搅拌5分钟至均匀完全；
步骤4：温度降至45℃时，再将抗菌肽加入，维持中速搅拌5分钟至均匀完全，即得产品。
本发明具有以下有益效果：
(1)本发明提供的抗菌肽洗手液利用金环蛇抗菌肽 Cathelicidin-BF作为本发明洗手液中的主要抑菌剂，具有广谱抗菌的功能。
(2)本发明提供的抗菌肽洗手液以月桂醇聚醚酸酯钠、椰油酰胺丙基甜菜碱等作为表面活性剂，添加适量的乙二醇二硬脂酸酯等作为乳化剂，该配伍具有良好的发泡、稳泡、渗透去污和抗硬水等功能，增稠乳化的同时增强了清洁去污效果。
(3)本发明提供的抗菌肽洗手液添加适量甘油等作为保湿剂，起到护手亲肤的作用，降低洗手液对手部皮肤的刺激；添加柠檬酸等 pH调节剂，调节该洗手液pH为5.5-6.5，与人体皮肤的酸碱度接近，保证洗手液在硬水中具有较好的洗涤效果同时减轻了对皮肤的刺激。
(4)本发明提供的抗菌肽洗手液制备过程工艺简单易操作，绿色环保，具有良好的经济效益。
具体实施方式
以下通过具体实施方式进一步描述本发明，但不应将此理解为本发明上述主题的范围仅限于以下实施例。
实施例1抗菌肽洗手液
主要成分含量见表1。抗菌肽洗手液的制备方法步骤如下：
步骤1：取1/4水加入乳化剂组份加热，中速均质搅拌溶解；
步骤2：再取1/4水溶解表面活性剂组份，然后加入保湿剂组份以真空高速搅拌溶解；
步骤3：将步骤1和步骤2的溶液混合，加入用剩余水溶解好的pH 调节剂，维持中速搅拌5分钟至均匀完全；
步骤4：温度降至45℃时，再将抗菌肽加入，维持中速搅拌5分钟至均匀完全，即得产品。
表1抗菌肽洗手液的主要成分含量
| 成分 | 质量百分比 |
| 抗菌肽 | 0.2％ |
| 月桂醇聚醚硫酸酯钠 | 13％ |
| 椰油酰胺丙基甜菜碱 | 5％ |
| 椰油酰甲基牛磺酸牛磺酸钠 | 5％ |
| 椰油酰胺 | 1.5％ |
| 乙二醇二硬脂酸酯 | 2％ |
| EDTA二钠 | 0.05％ |
| 柠檬酸 | 0.3％ |
| 甘油 | 4％ |
| 去离子水 | 68.5％ |
实施例2抗菌肽洗手液
主要成分含量见表2。抗菌肽洗手液的制备方法步骤同实施例1。
表2抗菌肽洗手液
| 商品名 | 质量百分比 |
| 抗菌肽 | 2.5％ |
| 月桂醇聚醚硫酸酯钠 | 13％ |
| 椰油酰胺丙基甜菜碱 | 5％ |
| 椰油酰胺 | 10％ |
| PEG-100硬脂酸酯 | 1％ |
| 乙二醇二硬脂酸酯 | 2％ |
| EDTA二钠 | 0.1％ |
| 柠檬酸 | 0.3％ |
| 甘油 | 1％ |
| 双丙甘醇 | 0.5％ |
| 去离子水 | 64.6％ |
实施例3抗菌肽洗手液
主要成分含量见表3。抗菌肽洗手液的制备方法步骤同实施例1。
表3抗菌肽洗手液
对比例1无抗菌肽洗手液
主要成分含量见表4。无抗菌肽洗手液的制备方法步骤同实施例1。
表4无抗菌肽洗手液
对比例2市售抗菌洗手液
试验例1理化性质检测
分别对本发明实施例1-3和对比例1制得的洗手液进行理化性质检测，并与市售产品进行对比，结果见下表5所示：
表5洗手液理化指标检测结果
结果显示，实施例1-3和对比例1制得的洗手液与市售产品比较，粘度、pH和稳定性没有明显差异。
试验例2皮肤刺激性试验
选择50名女性志愿者，分为5组，分别为实施例1-3组，对比例1、2组，每组10名。对应给予本发明实施例1-3，对比例1、2 制得的洗手液。各组涂抹洗手液0.5mL，以双层纱布(2.5cm*2.5cm) 和聚乙烯薄膜覆盖，用无刺激性胶布和绷带加以固定，在涂抹后4h，用水清洗，除去残留物。分别去除受试物1h、6h、12h、24h、48h观察皮肤局部反应，按表6评分，并按表7判定皮肤刺激强度的级别，结果见下表8所示。
表6皮肤刺激反应评分标准评判
表7皮肤刺激性强度评价标准
| 皮肤刺激指数 | 刺激强度级别 |
| 0～＜0.5 | 无刺激性 |
| 0.5～＜2.0 | 轻刺激性 |
| 2.0～＜6.0 | 中等刺激性 |
| 6.0～＜8.0 | 强刺激性 |
表8皮肤刺激性试验结果
结果显示，本发明实施例1-3和对比例1制得的洗手液均无阳性反应出现，皮肤刺激性等级为0，说明本发明提供的洗手液对皮肤无刺激，产品温和。
试验例3洗手液杀菌效果测试
按照“GB15979-2002一次性使用卫生用品卫生标准”中附录C的方法来测试本发明实施例的抗菌洗手液的杀菌率，结果见表9：
表9本发明洗手液的杀菌率
杀菌率结果如表9所述，本发明抗菌肽洗手液对金黄色葡萄球菌、大肠杆菌、白色念珠菌的杀菌率均可达95％以上，与对比例1相比，杀菌效果提高了15％-20％，与市售洗手液相比，杀菌效果也更显著。
试验例4长效抑菌测试
随机选择各职业人员200例，随机分为5组，每组40人，分别使用实施例1-3、对比例1、2中的抗菌洗手液。
使用方法：常规普通肥皂洗手并用自来水进行冲洗，使用本产品 1mL于双手，均匀揉擦于手部，尤其是指尖和手指之间，保持30秒的接触时间，用自来水进行冲洗，自然干燥。
按照疾控中心(CDC)，感染控制组织所提出的中华人民共和国手部消毒技术规范2002中的测试方法。产品长效抑菌测试结果：使用3 分钟后、3小时后、4小时后的手部自然菌平均杀灭率见表10。
表10手部自然菌平均杀灭率(％)
| 时间 | 实施例1 | 实施例2 | 实施例3 | 对比例1 | 对比例2 |
| 3分钟 | 100％ | 100％ | 100％ | 93.3％ | 100％ |
| 3小时 | 99.1％ | 99.4％ | 99.9％ | 84.4％ | 91.4％ |
| 4小时 | 98.4％ | 98.9％ | 99.8％ | 78.2％ | 83.6％ |
由表10结果可得，本发明提供的抗菌肽洗手液洗液4h后，仍然具有良好的细菌杀灭率；对照例中，其长效抑菌性能不佳，说明抗菌肽与皮肤作用后，在皮肤表面形成一层抑菌保护膜，使抑菌效果更持久。
试验例5使用效果测试
试用该洗手液产品1个月，并统计其对于洗手液的清洁效果、护手作用、易漂性能进行评价，得到如下评价统计结果：
表11本实施例中洗手液的效果评价
根据表11结果，几乎所有测试人员对本发明产品的清洁效果表示认可，在护手功效方面，90％以上的测试人员表示认可，且认同本发明洗手液节水易漂洗性能的人超过90％，均超过市售洗手液。因此，本发明的抗菌肽洗手液不仅清洁效果突出，并且具有较好的护手功效和易漂洗性能。
上述详细说明是针对本发明其中可行实施例的具体说明，该实施例并非用以限制本发明的范围，凡未脱离本发明所为的等效实施或变更，均应包含于本发明技术方案的范围内。
Claims (9)
1.一种抗菌肽洗手液，其特征在于，所述洗手液由抗菌肽、表面活性剂、保湿剂、乳化剂、pH调节剂以及去离子水组成。
2.如权利要求1所述的抗菌肽洗手液，其特征在于，所述洗手液成分的重量百分比为：抗菌肽0.2%-5%、表面活性剂20%-40%、保湿剂0.5%-5%、乳化剂2%-5%、pH调节剂0.05%-0.8%以及去离水45%-77%。
3.如权利要求2所述的抗菌肽洗手液，其特征在于，所述洗手液中成分的重量百分比为：抗菌肽0.5%-2%、表面活性剂20%-30%、保湿剂1%-3%、乳化剂2.5%-4%、pH调节剂0.1%-0.5%以及去离子水61%~75%。
4.如权利要求1-3任一所述的抗菌肽洗手液，其特征在于，所述抗菌肽是金环蛇抗菌肽Cathelicidin-BF。
5.如权利要求1-3任一所述的抗菌肽洗手液，其特征在于，所述表面活性剂选自月桂醇聚醚硫酸酯钠、椰油酰胺丙基甜菜碱、椰油酰甲基牛磺酸牛磺酸钠、椰油酰胺中的一种或多种。
6.如权利要求1-3任一所述的抗菌肽洗手液，其特征在于，所述保湿剂选自丁二醇、甘油、水解透明质酸钠、海藻糖、1，5-戊二醇，赤藓糖醇，双丙甘醇中的一种或多种。
7.如权利要求1-3任一所述的抗菌肽洗手液，其特征在于，所述乳化剂选自鲸蜡硬脂基葡糖苷、鲸蜡硬脂醇、PEG-100硬脂酸酯、乙二醇二硬脂酸酯中的一种或多种。
8.如权利要求1-3任一所述的抗菌肽洗手液，其特征在于，所述pH调节剂选自EDTA二钠、柠檬酸、碳酸氢钾、精氨酸中的一种或多种。
9.一种制备权利要求1-3任一所述的抗菌肽洗手液的制备方法，其特征在于，制备包括以下步骤：
步骤1：取1/4去离子水加入乳化剂组份加热，中速均质搅拌溶解；
步骤2：再取1/4去离子水溶解表面活性剂组份，然后加入保湿剂组份以真空高速搅拌溶解；
步骤3：将步骤1和步骤2的溶液混合，加入用剩余去离子水溶解好的pH调节剂，维持中速搅拌5分钟至均匀完全；
步骤4：温度降至45℃时，再将抗菌肽加入，维持中速搅拌5分钟至均匀完全，即得产品。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010680313.1A CN113940895A (zh) | 2020-07-15 | 2020-07-15 | 一种抗菌肽洗手液及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010680313.1A CN113940895A (zh) | 2020-07-15 | 2020-07-15 | 一种抗菌肽洗手液及其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113940895A true CN113940895A (zh) | 2022-01-18 |
Family
ID=79326108
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010680313.1A Pending CN113940895A (zh) | 2020-07-15 | 2020-07-15 | 一种抗菌肽洗手液及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113940895A (zh) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101412753A (zh) * | 2008-09-27 | 2009-04-22 | 中国科学院昆明动物研究所 | 金环蛇抗菌肽cathelicidin-BF及其基因和应用 |
| CN101496773A (zh) * | 2009-02-25 | 2009-08-05 | 吉林大学 | 一种含林蛙抗菌肽的抗菌洗剂及其制备方法 |
| CN104490720A (zh) * | 2015-01-18 | 2015-04-08 | 新疆大学 | 一种抗菌肽洗手液及其制备方法 |
| CN107028848A (zh) * | 2017-04-01 | 2017-08-11 | 江西凝盛生物科技有限公司 | 一种免洗手天然绿色的杀菌消毒液 |
| CN108685750A (zh) * | 2018-07-18 | 2018-10-23 | 湖南科技学院 | 一种抗菌肽洗手液及其制备方法 |
-
2020
- 2020-07-15 CN CN202010680313.1A patent/CN113940895A/zh active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101412753A (zh) * | 2008-09-27 | 2009-04-22 | 中国科学院昆明动物研究所 | 金环蛇抗菌肽cathelicidin-BF及其基因和应用 |
| CN101496773A (zh) * | 2009-02-25 | 2009-08-05 | 吉林大学 | 一种含林蛙抗菌肽的抗菌洗剂及其制备方法 |
| CN104490720A (zh) * | 2015-01-18 | 2015-04-08 | 新疆大学 | 一种抗菌肽洗手液及其制备方法 |
| CN107028848A (zh) * | 2017-04-01 | 2017-08-11 | 江西凝盛生物科技有限公司 | 一种免洗手天然绿色的杀菌消毒液 |
| CN108685750A (zh) * | 2018-07-18 | 2018-10-23 | 湖南科技学院 | 一种抗菌肽洗手液及其制备方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2465891C2 (ru) | Антибактериальные композиции, продукты и способы их применения | |
| CN100374099C (zh) | 抗微生物组合物 | |
| KR101426744B1 (ko) | 항바이러스제 및 세정제 | |
| CN101810541B (zh) | 一种消毒沐浴露 | |
| CN111110577B (zh) | 一种温和型除螨止痒沐浴露及其制备方法 | |
| US20060074029A1 (en) | Topical antimicrobial composition having improved moisturization properties | |
| CN109125096A (zh) | 泡沫洗手液及其制备方法 | |
| CN101548983A (zh) | 一种皮肤消毒剂及其制备方法 | |
| CN111184675A (zh) | 一种免洗消毒组合物及其制备方法与免洗消毒啫喱 | |
| CN111228201A (zh) | 一种免洗手凝胶的制备方法 | |
| CN105255596A (zh) | 一种含有艾叶提取物的抗菌洗洁精 | |
| CN111297730A (zh) | 长效无害免洗不易燃高分子乙醇消毒剂 | |
| KR20150001021A (ko) | 피부자극이 없는 방부제 조성물을 함유하는 물티슈 | |
| CN102293719A (zh) | 一种洗手液 | |
| CN101966134B (zh) | 一种杀菌护肤洗手液及其制备方法 | |
| CN113940895A (zh) | 一种抗菌肽洗手液及其制备方法 | |
| WO2021259211A1 (en) | Antiseptic compositions | |
| CN108300603A (zh) | 一种玩具清洗液及其制备方法 | |
| CN103961270B (zh) | 一种净碳防污隐形防护液及其制备方法 | |
| AU2013289294A1 (en) | Use of zinc coceth sulfate as an antibacterial agent against Propionibacterium acnes | |
| CN112716820A (zh) | 一种抗菌护肤免洗剂及其制备方法 | |
| CN111228145A (zh) | 一种温和抗菌洗手液及其制备方法 | |
| CN105535014A (zh) | 皮肤手消毒剂 | |
| KR20210142231A (ko) | 알칸디올 또는 에틸헥실글리세린을 포함하는 세정제 조성물 | |
| EP3106172B1 (de) | Antimikrobiell aktive mittel und deren verwendung |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220118 |