CN113940329B - Collecting and transferring device suitable for southern orius minutus physiological enzyme determination and using method - Google Patents
Collecting and transferring device suitable for southern orius minutus physiological enzyme determination and using method Download PDFInfo
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- CN113940329B CN113940329B CN202111388626.0A CN202111388626A CN113940329B CN 113940329 B CN113940329 B CN 113940329B CN 202111388626 A CN202111388626 A CN 202111388626A CN 113940329 B CN113940329 B CN 113940329B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M3/00—Manual implements, other than sprayers or powder distributors, for catching or killing insects, e.g. butterfly nets
- A01M3/005—Manual suction tools for catching insects
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Abstract
The invention discloses a collecting and transferring device suitable for the determination of physiological enzyme of orius miri and a using method thereof, and relates to the technical field of insect collecting and transferring. Including collection portion and transfer portion, collection portion is including storing up worm pipe, intake pipe and outlet duct, the intake pipe set up in store up the worm socle portion, the outlet duct set up in store up the worm socle top, transfer portion includes a transfer pipe and No. two transfer pipes, a transfer pipe and No. two transfer pipes set up respectively in store up the worm socle both sides. According to the collecting and transferring device provided by the invention, the top covers are arranged at the tops of the first transferring pipe and the second transferring pipe, so that the inside of the pest storing pipe is ensured to be clean in the process of sucking the orius similis, and the escape of pests in the pest storing pipe can be prevented.
Description
Technical Field
The invention relates to the technical field of insect collection and transfer, in particular to a collection and transfer device suitable for the determination of physiological enzyme of orius miri and a use method thereof.
Background
The use of natural enemies for pest control belongs to one of the biological pest control measures, and is gradually an important measure for comprehensive pest control because the natural enemies conform to the environment-friendly ecological strategy. The Orius miniatus has a natural enemy insect belonging to the family of Hemiptera (Hemiptera) oricaceae, and is one of the dominant natural enemies of agricultural systems in southern regions of China. Research shows that the orius miniatus has strong activity, can prey on various pests such as thrips, spider mites, aphids, whiteflies and the like, is widely distributed in the ecological environment of farmlands in south China, and has great utilization value. And the physiological enzyme assay of the orius pollidipennis can further reveal the adaptability of the orius pollidipennis to different environments, and provide effective reference for pest control such as thrips, aphids and the like.
At present, the collection and transfer mode used for the research of the physiological enzyme determination of the orius pollidii is mainly to transfer the orius pollidii to a required test device after being absorbed by an insect suction pipe, but the problems that the orius pollidii has small size and strong mobility, the transfer mode used at present is not perfect enough and the like cause the problems that the orius pollidii escapes or is difficult to transfer out from the insect suction pipe and the like in the transfer process, so that the problems that the amount of the orius pollidii required by the test is insufficient, the quality is inaccurate and the like are caused, and the test result of the physiological enzyme determination of the orius pollidii is finally influenced.
When the physiological enzyme of the orius pollidii is measured, the orius pollidii needs to be collected and absorbed from a living plant of the orius pollidii, and is transferred to a centrifugal tube to be weighed for subsequent measurement such as protein extraction, ice bath homogenization, centrifugation and the like, however, the insect suction tube used at present has a common problem no matter an oral suction type insect suction tube or an automatic insect suction tube: after sucking the pest body to the pest sucking pipe, the pest sucking pipe only has an export that shifts, and this export is generally bigger than the mouth of pipe of centrifuging tube moreover, so the orius similis is difficult to all to be shifted to the centrifuging tube, and some polypus similis still can remain in the pest sucking pipe sometimes, and on the contrary, if this export is less than the mouth of pipe of centrifuging tube, then the orius similis that shifts to among centrifuging tube or other experimental facilities escapes from the space again easily. Therefore, the fact that the aperture of the outlet of the insect suction pipe cannot be changed to fit a centrifugal pipe or other collecting devices is the main reason that the orius similis is easy to escape in the transferring process, and accurate results are difficult to obtain in physiological enzyme determination tests.
Disclosure of Invention
The invention mainly aims to provide a collecting and transferring device suitable for the physiological enzyme assay of orius pollidii and a using method thereof, so as to solve the problems in the prior art.
In order to solve the technical problems, the invention adopts the following technical scheme:
the utility model provides a collect transfer device suitable for southern orius minutus physiological enzyme survey, includes collection portion and transfer portion, collection portion is including storing up worm pipe, intake pipe and outlet duct, the intake pipe set up in store up the worm socle portion, the outlet duct set up in store up the worm pipe top, transfer portion includes a transfer pipe and No. two transfer pipes, a transfer pipe and No. two transfer pipes set up respectively in store up the worm pipe both sides.
Furthermore, a gauze is arranged at the connecting position of the air outlet pipe and the insect storage pipe, and the mesh number of the gauze is 110-150 meshes.
Furthermore, the exposed end of the air outlet pipe is provided with threads for being in threaded connection with an oral suction type pest sucking pipe or a pest sucking machine.
Furthermore, the top of a transfer pipe is provided with a top cover, and the top cover is in threaded connection with the transfer pipe.
Furthermore, the top of No. two transfer pipes is equipped with No. two top caps, No. two top caps with No. two transfer pipe threaded connection.
Furthermore, the caliber of the first transfer tube is 10mm, and is consistent with that of a centrifuge tube used for the determination of the physiological enzyme of the orius pollidipennis.
Furthermore, the caliber of the second transfer tube is 20mm-40 mm.
Further, the pest storage tube is of a regular triangular prism structure, the side length of the bottom surface of the regular triangular prism is 60mm, the side edge length is 20mm, and the vertex angle is of an arc-shaped structure.
Furthermore, the insect storage tube is made of transparent materials.
A use method of a collecting and transferring device suitable for the physiological enzyme assay of orius minutus comprises the following steps:
s1: when the device is used, the oral suction insect suction pipe or the insect suction machine is selected according to the number of the orius minutus, the oral suction insect suction pipe or the insect suction machine is connected to the air outlet pipe in a threaded mode, then the first top cover of the first transfer pipe and the second top cover of the second transfer pipe are screwed tightly in a rotating mode respectively, and assembly is finished;
s2: when the mouth suction type insect suction pipe is used, the air inlet pipe is held by hand, then the air inlet pipe is aligned to the orius pollidii, and the orius pollidii is sucked into the insect storage pipe; when the insect suction machine is used, the air inlet pipe is held by the left hand, the insect suction machine is held by the right hand, the switch of the insect suction machine is opened by the right hand after the air inlet pipe is aligned with the orius pollidipenus, and the orius pollidipenus is sucked into the insect storage pipe;
s3: after sucking a sufficient number of orius pollidii, vertically moving the first transfer tube upwards, returning the orius pollidii crawling on the wall of the first transfer tube to the insect storage tube in a vibration mode, then rotating and unscrewing the first top cover of the first transfer tube, aligning the tube opening of the first transfer tube with the tube opening of a centrifugal tube used for the test, and then transferring the orius pollidii to the centrifugal tube in a vibration mode;
s4: after a sufficient number of orius mirificus were transferred in the same manner, the subsequent physiological enzyme assay procedure was performed.
Compared with the prior art, the collecting and transferring device suitable for the physiological enzyme assay of the orius similis and the using method provided by the invention have the following beneficial effects:
the pest storage tube is a transparent regular triangular prism structure with a slightly arc-shaped vertex angle, and the design can reduce the friction force of the orius pollidipennis or other insects in the pest storage tube, so that the orius pollidipennis or other insects can be transferred out of the transfer tube under the action of gravity; the transparent material design can clearly see the movement condition and the quantity of the orius similis in the insect storage pipe, in addition, the two transfer pipes with different calibers are connected to the insect storage pipe and can be matched with test equipment with different calibers, and the top parts of the transfer pipes are provided with top covers, so that the cleanness of the interior of the insect storage pipe in the process of sucking the orius similis or other insects can be ensured, the escape of insects in the insect storage pipe can be prevented, the calibers of one transfer pipe is matched with the calibers of a centrifugal pipe used for measuring the physiological enzyme of the orius similis, the escape of the orius similis in the transfer process is ensured, unnecessary operation in the test is reduced, and the test accuracy is improved.
Drawings
FIG. 1 is a top view of the present invention.
FIG. 2 is a schematic view of the structure of the connection between the insect storage tube and the air outlet tube.
Figure 3 is a time line graph of different devices used to aspirate orius pollicis.
The device comprises a shell, a shell cover and a gauze, wherein the shell cover 1-storage tube, the shell cover 2, the shell cover 3-first transfer tube, the shell cover 4-second transfer tube, the shell cover 6-first top cover, the shell cover 6-first cover, the shell cover, the 8-gauze.
Detailed Description
The technical solution of the present invention is further illustrated by the accompanying drawings and examples.
With reference to fig. 1 to 3, the invention provides a collecting and transferring device suitable for southern orius minutus physiological enzyme determination, which comprises a collecting part and a transferring part, wherein the collecting part comprises an insect storage tube 1, an air inlet tube 2 and an air outlet tube 5, the air inlet tube 2 is arranged at the bottom of the insect storage tube 1, the air outlet tube 5 is arranged at the top of the insect storage tube 1, the transferring part comprises a first transferring tube 3 and a second transferring tube 4, and the first transferring tube 3 and the second transferring tube 4 are respectively arranged at two sides of the insect storage tube 1. In this embodiment, the air inlet pipe 2 is a hose, and when the orius pollidii does not need to be sucked or transferred, the orius pollidii can be knotted, so that the orius pollidii or other polypide in the pest storage pipe 1 is prevented from escaping from the air inlet 2.
Store up worm pipe 1 and be positive triangular prism structure, positive triangular prism bottom surface length of side is 60mm, and the length of side edge is 20mm, and the apex angle is the arc structure. When enough orius similis is sucked, the outlet of the first transfer tube 3 or the second transfer tube 4 is aligned to a centrifuge tube or other required equipment, the orius similis can be transferred in a gravity and artificially added vibration mode, and the structure and the vertex angle of the triangular prism of the insect storage tube 1 are provided with radians, so that the orius similis is difficult to escape to other positions, and the transfer speed can be accelerated. The insect storage tube 1 is made of transparent materials. In the embodiment, the insect storage tube 1 is made of polymethyl methacrylate, so that the transparent property is good, and the condition of insects can be observed conveniently from the outside.
Furthermore, a gauze is arranged at the connecting position of the air outlet pipe and the insect storage pipe, and the mesh number of the gauze is 110-150 meshes. The gauze can ensure that the orius miniatus or other polypide can not be sucked into the air outlet pipe.
Furthermore, the exposed end of the air outlet pipe is provided with threads for being in threaded connection with an oral suction type pest sucking pipe or a pest sucking machine. The thread structure is characterized in that the thread structure can be connected with an automatic insect sucking machine and can also be connected with an oral suction insect sucking pipe, when the number of the orius pollidii is small or other smaller insects need to be sucked, the oral suction insect sucking pipe can be connected, and the situation that the sucked insects are injured or die due to overlarge suction force of the insect sucking machine is prevented. When insects with larger individuals or more quantity or after drug treatment need to be sucked, the sucking machine can be connected, so that the efficiency and the safety of sucking insects are improved.
Furthermore, a top cover 6 is arranged at the top of the first transfer pipe 3, and the first top cover 6 is in threaded connection with the first transfer pipe 3. The top of No. two transfer tubes 4 is equipped with No. two top caps 7, No. two top caps 7 with No. two transfer tubes 4 threaded connection. First top cap 6 and No. two top caps 7 accessible rotation is screwed up the installation or is dismantled, is convenient for shift and prevents the escape of the tiny flower stinkbug.
Further, the caliber of the first transfer tube 3 is 10mm, and is consistent with the caliber of a centrifuge tube used for the determination of the physiological enzyme of the orius pollidipennis.
Furthermore, the caliber of the second transfer tube 4 is 20mm-40 mm. The device is used for matching with other test equipment with larger caliber.
A use method of a collecting and transferring device suitable for the physiological enzyme assay of orius minutus comprises the following steps:
s1: when the device is used, the oral suction insect suction pipe or the insect suction machine is selected according to the number of the orius minutus, the oral suction insect suction pipe or the insect suction machine is connected to the air outlet pipe 5 through threads, then the first top cover 6 of the first transfer pipe 3 and the second top cover 7 of the second transfer pipe 4 are respectively screwed tightly in a rotating mode, and the assembly is finished;
s2: when the mouth suction type insect suction pipe is used, the air inlet pipe 2 is held by hand and then is aligned with the orius pollidipennis, and the orius pollidipennis can be sucked into the insect storage pipe 1; when the insect suction machine is used, the air inlet pipe 2 is held by the left hand, the insect suction machine is held by the right hand, the switch of the insect suction machine is opened by the right hand after the air inlet pipe 2 is aligned with the southern orius bugs, and then the southern orius bugs can be sucked into the insect storage pipe 1;
s3: after sucking a sufficient number of orius pollidii, vertically upwards moving the first transfer tube 3, enabling the orius pollidii which crawls on the wall of the first transfer tube 3 to return to the insect storage tube 1 in a vibration mode, then rotatably unscrewing the first top cover 6 of the first transfer tube 3, aligning the tube opening of the first top cover with the tube opening of a centrifugal tube used for the test, and then transferring the orius pollidii into the centrifugal tube in a vibration mode;
s4: after transferring a sufficient number of orius mirificus in the same manner, the subsequent physiological enzyme assay operation can be performed.
The effective effect of the invention is further clarified by combining specific experimental data, the orius pollidii on the wild pea flower is selected for the experiment, and the device and the traditional pest suction pipe are adopted for collecting respectively.
And (3) test operation: the traditional insect suction pipe and the device are respectively adopted to collect and transfer the orius pollidipennis on the wild pea flowers, and the time for collecting the orius pollidipennis, the time for transferring the orius pollidipennis and the loss amount are respectively recorded.
The traditional insect suction pipe device and the device are respectively used for carrying out four times of tests to suck the orius pollidipennis under the same indoor conditions, 10 pieces of the orius pollidipennis are sucked in the first test, 50 pieces of the orius pollidipennis are sucked in the second test, 100 pieces of the orius pollidipennis are sucked in the third test, and 150 pieces of the orius pollidipennis are tested in the fourth test, and it can be seen from a graph (1) that in the four times of tests, the time for sucking the orius pollidipennis by the device is shorter than the time for sucking the orius pollidipennis by the traditional insect suction pipe, the time difference for sucking the orius pollidipennis by the two devices is gradually increased along with the increase of the sucking quantity, and when the collecting quantity is increased, the advantage of the device on the sucking speed is gradually obvious. Compared with the traditional insect suction pipe device, the insect storage pipe of the device has a triangular prism structure, and the design of the vertex angle is favorable for reducing the re-escape of the orius similis from the air inlet pipe, so that the time consumed in collection is relatively reduced.
Time taken for transferring orius pollidii by different devices
TABLE 1 time taken for different devices to transfer orius pollidii
Note: values in the figure are mean ± sem. The same column of different lower case letters indicates that the transfer time difference of the same quantity transferred by two different transfer devices is obvious (P <0.01, Duncan's new double-pole difference method);
as can be seen from the data in table (1), the present device and the conventional device were used to transfer the orius pollidii, perform three experiments, aspirate and transfer the orius pollidii 10 heads, 50 heads, 100 heads into the centrifuge tube used for physiological enzyme assay, with a time that was significantly shorter than the conventional insect suction tube device (F1, 24-49.691, P < 0.01). The pest storage tube of the device is a transparent regular triangular prism structure with a slightly arc-shaped vertex angle, so that friction force of the orius similis or other insects in the pest storage tube can be reduced, the orius similis or other insects can be transferred out of the transfer tube under the action of gravity, and the orius similis can be transferred conveniently due to the design that the caliber of the first transfer tube is consistent with that of a centrifugal tube used for physiological enzyme determination, so that the time consumed by transfer can be reduced.
Transferring loss quantity of orius miri by different devices
Table 2 transfer of orius miri losses by different devices
Note: values in the figure are mean ± sem. The same column of different lower case letters indicates that the difference of the lost quantity of the same quantity transferred by two different transfer devices is obvious (P <0.01, Duncan's new double-pole difference method);
as can be seen from the data in table (2), the traditional insect suction tube device and the device of the present invention are used for transferring the orius pollidipennis, three times of experiments are carried out, 10 heads, 50 heads and 100 heads of the orius pollidipennis are sucked and transferred to a centrifugal tube used for physiological enzyme determination, and the loss amount of death, escape and the like in the transfer process is very significantly smaller than that of the traditional insect suction tube device (F1,24 ═ 77.312, P < 0.01). Although the device has some loss, compared with the traditional insect suction tube device, the device has greatly improved defects in the aspect, because the caliber of the mouth of the traditional insect suction tube is fixed and is slightly larger than that of a centrifugal tube used for measuring the physiological enzyme of the southern orius minutus, the device is very inconvenient in the transfer process and the southern orius minutus is easy to escape, and the device is specially provided with a first transfer tube, and the caliber of the mouth of the transfer tube is consistent with that of the centrifugal tube used for measuring the physiological enzyme of the southern orius minutus, so that the loss of the southern orius minutus in the transfer process can be reduced.
The experiment shows that the collecting and transferring device adopted by the invention has obvious advantages in transferring the orius similis compared with the traditional insect suction pipe. Meanwhile, the mouth-sucking type or insect-sucking gun is simple to operate and convenient to carry, the insect-sucking mode can be selected, the problems that the traditional mouth-sucking type insect-sucking pipe is slow in insect-sucking speed, the insect-sucking gun is easy to cause insect injury and the like are solved, and powerful support is provided for the physiological enzyme determination of the orius miniatus.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the technical scope of the present invention, so that any minor modifications, equivalent changes and modifications made to the above embodiment according to the technical spirit of the present invention are within the technical scope of the present invention.
Claims (5)
1. The collecting and transferring device is suitable for the physiological enzyme determination of the orius minutus and is characterized by comprising a collecting part and a transferring part, wherein the collecting part comprises an insect storage pipe, an air inlet pipe and an air outlet pipe;
the pest storage pipe is of a regular triangular prism structure, the side length of the bottom surface of the regular triangular prism is 60mm, the side edge length is 20mm, and the vertex angle is of an arc structure;
the top of the first transfer pipe is provided with a first top cover, and the first top cover is in threaded connection with the first transfer pipe;
a second top cover is arranged at the top of the second transfer pipe and is in threaded connection with the second transfer pipe;
the caliber of the first transfer tube is 10mm, and is consistent with that of a centrifugal tube used for the determination of the physiological enzyme of the orius minutus;
the caliber of the second transfer tube is 20mm-40 mm.
2. The collecting and transferring device suitable for the physiological enzyme assay of the orius minutus according to claim 1, wherein a gauze is arranged at the connecting position of the air outlet pipe and the insect storage pipe, and the mesh number of the gauze is 110-150 meshes.
3. The collecting and transferring device suitable for the physiological enzyme assay of the orius minutus according to claim 2, wherein the exposed end of the air outlet pipe is provided with threads for being in threaded connection with an oral suction type insect suction pipe or an insect suction machine.
4. The collection and transfer device suitable for the physiological enzyme assay of orius minutus according to claim 1, wherein the insect storage tube is made of a transparent material.
5. A method of using a collection and transfer device suitable for use in the southern orius bug physiological enzyme assay of any of claims 1 to 4, comprising the steps of:
s1: when the device is used, the oral suction insect suction pipe or the insect suction machine is selected according to the number of the orius minutus, the oral suction insect suction pipe or the insect suction machine is connected to the air outlet pipe in a threaded mode, then the first top cover of the first transfer pipe and the second top cover of the second transfer pipe are screwed tightly in a rotating mode respectively, and assembly is finished;
s2: when the mouth suction type insect suction pipe is used, the air inlet pipe is held by hand, then the air inlet pipe is aligned to the orius pollidii, and the orius pollidii is sucked into the insect storage pipe; when the insect suction machine is used, the air inlet pipe is held by the left hand, the insect suction machine is held by the right hand, the switch of the insect suction machine is opened by the right hand after the air inlet pipe is aligned with the orius pollidipenus, and the orius pollidipenus is sucked into the insect storage pipe;
s3: after sucking a sufficient number of orius pollidii, vertically moving the first transfer tube upwards, returning the orius pollidii crawling on the wall of the first transfer tube to the insect storage tube in a vibration mode, then rotating and unscrewing the first top cover of the first transfer tube, aligning the tube opening of the first transfer tube with the tube opening of a centrifugal tube used for the test, and then transferring the orius pollidii to the centrifugal tube in a vibration mode;
s4: after transferring a sufficient number of orius mirificus in the same manner, the subsequent physiological enzyme assay operation can be performed.
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