CN113933134A - Staining method for staining sample and related equipment - Google Patents
Staining method for staining sample and related equipment Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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Abstract
The embodiment of the application provides a dyeing method and related equipment for sample dyeing, which are used for intelligently adjusting dyeing parameters so as to meet dyeing requirements under various scenes. The embodiment of the application provides a staining method for sample staining, which is applied to a slide staining device, wherein the slide staining device comprises a slide pushing mechanism, a slide fixing mechanism and a staining mechanism, wherein the slide pushing mechanism is used for loading a sample to be detected on a slide and smearing the sample to be detected to prepare a sample smear; the staining mechanism is used for staining the sample to be detected on the sample smear; a transport mechanism for transporting the sample smear from the blade pushing mechanism to the staining mechanism; the specific operation is as follows: obtaining sample information of the sample to be detected; controlling the sheet pushing mechanism to prepare a sample smear according to the sample information; controlling the transport mechanism to transport the sample smear from the sheet pushing mechanism to the staining mechanism; and controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear.
Description
Technical Field
The present application relates to the field of medical devices, and in particular, to a staining method for staining a sample and related apparatus.
Background
Microscopy of sample smears is the fundamental method of sample examination and has extremely wide applications. Especially, the diagnosis of various blood diseases is of great value. The general staining procedure is shown in FIGS. 1 and 2. Wherein FIG. 1 shows a methanol fixed dyeing process, and FIG. 2 shows a non-methanol fixed dyeing process. In both staining procedures, the staining parameters were the same for all sample smears.
In practical application, different cells have different internal structures, and the requirements for the depth of staining are different. If the dyeing is carried out by adopting the same dyeing parameters, the dyeing requirement cannot be met.
Disclosure of Invention
The embodiment of the application provides a dyeing method and related equipment for sample dyeing, which are used for intelligently adjusting dyeing parameters so as to meet dyeing requirements under various scenes.
In a first aspect, the present application provides a staining method for staining a sample, which is applied to a slide staining apparatus, where the slide staining apparatus includes a slide pushing mechanism for smearing a sample to be detected on a slide to make a sample smear; the staining mechanism is used for staining the sample to be detected on the sample smear; a transport mechanism for transporting the sample smear from the blade pushing mechanism to the staining mechanism; the specific operation is as follows: obtaining sample information of the sample to be detected; controlling the sheet pushing mechanism to prepare a sample smear according to the sample information; controlling the transport mechanism to transport the sample smear from the sheet pushing mechanism to the staining mechanism; and controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear.
In a second aspect, an embodiment of the present application provides a staining method for staining a sample, which is applied to a staining apparatus including a staining mechanism, where the staining mechanism is configured to stain a sample to be tested on a sample smear, and the method specifically includes the following operations: obtaining sample information of a sample to be detected; and controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear.
In a third aspect, an embodiment of the present application provides a push-piece dyeing apparatus, including:
the slide pushing mechanism is used for loading a sample to be detected on a slide and smearing the sample to prepare a sample smear;
a staining mechanism for staining a sample on the sample smear, the staining mechanism having a special staining mode and a general staining mode;
a transport mechanism for transporting the sample smear to a staining mechanism;
a control mechanism in communicative connection with the blade pushing mechanism, the conveying mechanism, and the dyeing mechanism, configured to: determining an operation mode of the dyeing mechanism from a special dyeing mode and a common dyeing mode, controlling the dyeing mechanism to dye a sample to be detected on the sample smear according to a first dyeing parameter when the operation mode is determined to be the special dyeing mode, determining the first dyeing parameter according to a preset mapping relation table, wherein the preset mapping relation table is used for indicating the corresponding relation between the sample and the dyeing parameter, and controlling the dyeing mechanism to dye the sample to be detected on the sample smear according to a conventional dyeing parameter when the operation mode is determined to be the common dyeing mode, wherein the conventional dyeing parameter is a dyeing parameter preconfigured by the slide-pushing dyeing device.
In a fourth aspect, an embodiment of the present application provides a sample staining apparatus, including:
a staining mechanism for staining a sample on a sample smear, the staining mechanism having a special staining mode and a general staining mode; a control mechanism, communicatively coupled to the dyeing mechanism, configured to: determining an operation mode of the staining mechanism from a special staining mode and a common staining mode, controlling the staining mechanism to stain a sample to be detected on the sample smear according to a first staining parameter when the operation mode is determined to be the special staining mode, determining the first staining parameter according to the preset mapping relation table, wherein the preset mapping relation table is used for indicating the corresponding relation between the sample and the staining parameter, and controlling the staining mechanism to stain the sample to be detected on the sample smear according to a conventional staining parameter when the operation mode is determined to be the common staining mode, wherein the conventional staining parameter is a staining parameter preconfigured by the staining device.
In a fifth aspect, an embodiment of the present application provides a sample analysis system, where the sample analysis system includes:
the sample analysis device is used for detecting a sample to be detected and obtaining sample information;
the push piece dyeing device comprises a push piece mechanism and a dyeing mechanism, wherein the push piece mechanism is used for preparing a sample smear, and the dyeing mechanism is used for dyeing a sample to be detected on the sample smear;
the cell image analysis device is used for shooting and analyzing the sample smear;
a transport device comprising a first transport track for transporting a sample from the sample analysis device to the blade pushing mechanism, a second transport track for transporting a sample smear from the blade pushing mechanism to the staining mechanism, and a third transport track for transporting a sample smear from the staining mechanism to the cellular image analysis device; and
a control mechanism communicatively coupled to the sample analysis device, the blade pushing mechanism, the staining mechanism, the cell image analysis device, and the transport device and configured to:
obtaining sample information of a sample from the sample analysis device;
controlling the first conveying track to convey the sample to be detected to the sheet pushing mechanism; controlling the sheet pushing mechanism to prepare a sample smear and controlling the second conveying track to convey the sample smear to the dyeing mechanism; controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear; and controlling the third conveying track to convey the sample smear from the dyeing mechanism to the cell image analysis device for shooting and analysis.
According to the embodiment of the application, the sample dyeing parameters are determined according to the sample information, and the dyeing parameters are intelligently adjusted, so that the dyeing requirements under various scenes are met.
Drawings
FIG. 1 is a schematic diagram of a methanol fixed dyeing process;
FIG. 2 is a schematic of a non-methanol fixed dyeing process;
FIG. 3 is a schematic structural diagram of a slide dyeing apparatus according to an embodiment of the present application;
FIG. 4 is a schematic structural view of another slide dyeing apparatus according to an embodiment of the present application;
FIG. 5 is a schematic view of another embodiment of the slide dyeing apparatus of the present application;
FIG. 6 is a flow chart of a specimen smear slide staining procedure of the slide staining apparatus according to the embodiment of the present application;
FIG. 7 is a schematic diagram of an embodiment of a staining method for staining a sample in an embodiment of the present application;
FIG. 8 is a schematic diagram of another embodiment of a staining method for staining a sample in an embodiment of the present application;
FIG. 9 is a schematic view of the operation of the slide dyeing apparatus according to the embodiment of the present application;
FIG. 10 is a schematic view of another operation of the slide dyeing apparatus according to the embodiment of the present application;
fig. 11 is a schematic structural diagram of a sample analysis system in an embodiment of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the embodiments of the present application are described below with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present application, but not all embodiments. As can be known to those skilled in the art, with the advent of new application scenarios, the technical solution provided in the embodiments of the present application is also applicable to similar technical problems.
The terms "first," "second," and the like in the description and in the claims of the present application and in the above-described drawings are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It will be appreciated that the data so used may be interchanged under appropriate circumstances such that the embodiments described herein may be practiced otherwise than as specifically illustrated or described herein. Moreover, the terms "comprises," "comprising," and any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or modules is not necessarily limited to those steps or modules explicitly listed, but may include other steps or modules not expressly listed or inherent to such process, method, article, or apparatus. The naming or numbering of the steps appearing in the present application does not mean that the steps in the method flow have to be executed in the chronological/logical order indicated by the naming or numbering, and the named or numbered process steps may be executed in a modified order depending on the technical purpose to be achieved, as long as the same or similar technical effects are achieved. The division of the units presented in this application is a logical division, and in practical applications, there may be another division, for example, multiple units may be combined or integrated into another system, or some features may be omitted, or not executed, and in addition, the shown or discussed coupling or direct coupling or communication connection between each other may be through some interfaces, and the indirect coupling or communication connection between the units may be in an electrical or other similar form, which is not limited in this application. Furthermore, the units or sub-units described as the separate parts may or may not be physically separate, may or may not be physical units, or may be distributed in a plurality of circuit units, and some or all of the units may be selected according to actual needs to achieve the purpose of the present disclosure.
For example, the sample analysis system provided in the embodiments of the present application includes a series of devices, but the sample analysis system provided in the embodiments of the present application is not limited to include the devices explicitly described. Similarly, the sample analysis method provided in the embodiments of the present application includes a series of steps, but the sample analysis method provided in the embodiments of the present application is not limited to the described steps. It should be noted that in the following description, reference is made to "embodiments" which describe a subset of all possible embodiments, but it is understood that "embodiments" may be the same subset or different subsets of all possible embodiments, and may be combined with each other without conflict.
The embodiment provides a staining method for staining a sample, wherein the sample to be detected comprises but is not limited to microorganisms, blood, body fluid, bone marrow fluid and the like. In order to more clearly express the inventive concept of the present application, a smear using blood as a sample and a smear preparing apparatus for making the smear are described below as an example. Before further detailed description of the present invention, terms and expressions referred to in the embodiments of the present invention are described, and the terms and expressions referred to in the embodiments of the present invention are applicable to the following explanations.
1, in response to the condition or state on which the operation to be performed depends being indicated, one or more operations to be performed may be in real time or may have a set delay when the condition or state on which the operation depends is satisfied; there is no restriction on the order of execution of the operations performed unless otherwise specified.
2, sample smear, i.e. a substrate on which a specimen is smeared.
And 3, dyeing parameters including dyeing duration, dye liquor and buffer liquor ratio and temperature. In this embodiment, the dyeing duration, the ratio of the dye solution to the buffer solution, and the temperature can be adjusted. Taking the white blood cells as an example, if the dyeing time of the dye solution and the buffer solution mixture is longer, the more the white blood cells are combined with the dye, the darker the white blood cells are; the higher the ratio of dye to buffer, the darker the staining of the leukocytes and vice versa.
The push piece dyeing device provided by the embodiment of the application is shown in fig. 3, and comprises a push piece mechanism 301, a slide sheet mechanism and a slide sheet mechanism, wherein the push piece mechanism 301 is used for loading a sample to be tested on a slide sheet and smearing the sample to prepare a sample smear;
a staining mechanism 303 for staining the sample on the sample smear, the staining mechanism having a special staining mode and a general staining mode;
a transport mechanism 302 for transporting the sample smear to a staining mechanism 303;
a control mechanism 304, communicatively connected to the blade pushing mechanism 301, the dyeing mechanism 303 and the conveying mechanism 302, configured to: determining an operation mode of the dyeing mechanism 303 from a special dyeing mode and a normal dyeing mode, when the operation mode is determined to be the special dyeing mode, controlling the dyeing mechanism 303 to dye the sample to be detected on the sample smear according to a first dyeing parameter, wherein the first dyeing parameter is determined according to a preset mapping relation table, the preset mapping relation table is used for indicating the corresponding relation between the sample and the dyeing parameter, when the operation mode is determined to be the normal dyeing mode, controlling the dyeing mechanism 303 to dye the sample to be detected on the sample smear according to a conventional dyeing parameter, and the conventional dyeing parameter is a dyeing parameter preconfigured by the slide-pushing dyeing device.
Referring to fig. 4 and 5, the smear dyeing apparatus of the present embodiment can be used for smear preparation of samples such as blood and body fluid and for dyeing sample smears. The slide staining apparatus includes a sampling mechanism 1 for taking a sample, a slide loading mechanism 2 (i.e., equivalent to the carrying mechanism 302 in fig. 3) for moving a slide to a working line, a loading mechanism 3 for loading the sample onto the slide, a slide pushing mechanism 4 (i.e., equivalent to the slide pushing mechanism 301 in fig. 3) for leveling the sample on the slide, a drying mechanism (not shown) for drying the sample on the slide, and a staining mechanism 5 (i.e., equivalent to the staining mechanism 303 in fig. 3) for staining the sample on the slide.
Referring to fig. 4 to 6, in an embodiment, when the sampling mechanism 1 performs sample extraction, the sample is first mixed, and then the sampling device (e.g. the sampling needle 31) in the sampling mechanism 1 is used to suck the sample, according to the difference of the sample container, the sample sucking may be a puncture sample sucking (the sample container has a cover, and the sampling device passes through the cover of the sample container), or an open sample sucking (the sample container is open, and the sampling device directly sucks the sample from the open portion). In some embodiments, the device further comprises a micro-sampling mechanism 6, and the micro-sampling mechanism 6 can directly move the test tube placed by the operator to the direction of the sampling device, or the sampling device can also move the test tube placed by the operator to the direction of the sampling device. In other implementation manners, the microinjection mechanism 6 can also directly move the test tube to the direction of the sampling mechanism 3, or the sampling mechanism 3 can also move to the direction of the test tube placed by the operator, and the sample loading is directly carried out after the blood sample is absorbed by the sampling mechanism 3 (for example, a blood dropping needle), so that the requirement of the blood sample can be reduced because the blood is not required to be extracted by the sampling mechanism 1, and thus the microinjection and the prior sampling are realized. When sampling is complete, the blood is ready to be dropped onto the slide via the sample application mechanism 3.
Accordingly, the slide loading mechanism 2 extracts the slide and loads the slide to the corresponding position to facilitate the blood dropping operation. In some embodiments, as shown in fig. 6, after the slide extraction operation is completed, the slide left-right detection and slide cleaning operations may be performed, and then the slide is loaded. The loaded slide can be printed with relevant information, and the front and back detection of the slide and the like can be carried out at the same time.
Referring to fig. 4 to 6, after the blood dropping needle of the sample adding mechanism 3 drops the sample onto the slide, the slide pushing mechanism 4 pushes the blood into a blood film shape on the slide. Generally, after the slide pushing operation is completed, the blood film on the slide is dried to stabilize the shape. As shown in fig. 6, in some embodiments, the slide may be driven to turn over before drying the blood membrane to meet the corresponding requirements. In some embodiments, the dried blood smear can be further subjected to a drying test to determine the drying effect of the blood film. In some embodiments, the dried blood smear can be further subjected to blood film spreading detection to determine whether the blood film is spread and whether the spreading state meets the requirements.
Continuing with FIGS. 4-6, after the smear is pushed, the smear can be stained by a staining mechanism 5, wherein the staining mechanism 5 has the function of the staining mechanism 303 shown in FIG. 3.
Fig. 3 to 6 describe a slide staining apparatus for implementing the sample staining method in the embodiment of the present application. It is understood that, in order to implement the sample staining method in the embodiment of the present application, a structure of a single push piece device and a single staining device may also be adopted. The single dyeing device may have the functions of the dyeing mechanism 303 described above, and will not be described herein again. The single blade pushing device also has the function of the blade pushing mechanism 301 in the above description, and will not be described herein again.
Specifically, referring to fig. 7, an embodiment of a staining method for staining a sample according to an embodiment of the present application includes:
701, obtaining sample information of a sample to be detected.
The slide-pushing dyeing device receives the sample information of the sample to be detected sent by the sample analyzer or directly obtains the sample information of the sample to be detected input by a user through input equipment.
In an embodiment of the present application, the sample information includes at least one item of information of each component in the sample to be tested, a sample type of the sample to be tested, source information of the sample to be tested, and application scenario information of the sample to be tested. The information on each component (which may be referred to as a cell detection parameter) in the sample includes hemoglobin information, leukocyte information, and platelet information. The cell detection parameter information can be used for indicating that the corresponding numerical value of the cell detection parameter is abnormal or normal. In this embodiment, the numerical value abnormality corresponding to the cell detection parameter mainly indicates that the numerical value corresponding to the cell detection parameter does not satisfy the corresponding threshold (for example, is greater than the threshold or is not within the threshold range), the cell detection parameter is default (for example, the cell detection parameter is empty or the numerical value corresponding to the cell detection parameter is an initial value), the numerical value corresponding to the cell detection parameter directly indicates whether there is an abnormality (for example, it is not required to determine by a threshold method), and the like all of which are considered as the numerical value abnormality corresponding to the cell detection parameter. Otherwise, the corresponding numerical value of the cell detection parameter is normal.
And the sample type of the sample to be tested is used for indicating that the sample to be tested is blood, body fluid or marrow fluid.
The source information of the sample to be detected comprises information of a person to be detected corresponding to the sample to be detected or a source department of the sample to be detected. For example, the historical case of the person to be detected shows that the person to be detected has leukemia; or the sample to be detected is from a specific department, for example, the detection result information is from but not limited to the procreation medical department, and the specific department may be determined according to medical needs, which is not limited in this embodiment.
The application scene information of the sample comprises a detection item corresponding to the sample. Such as the detection of only leukocytes or only platelets in the test sample, etc. The details are not limited herein.
And 702, controlling the sheet pushing mechanism to prepare a sample smear according to the sample information.
In this embodiment, the blade pushing and dyeing device can directly control the blade pushing mechanism to prepare a sample smear for the sample to be tested; or the push piece dyeing device determines the number of sample smears prepared by the push piece mechanism according to the sample information.
When the sheet pushing mechanism prepares a sample smear according to the sample information, the specific operation can be as follows: the push piece dyeing mechanism determines whether the sample to be detected is a special sample or an abnormal sample according to the sample information, and if so, the push piece dyeing device controls the push piece mechanism to prepare at least one sample smear for the sample to be detected; and if the sample information indicates that the sample to be detected is a normal sample, the push piece dyeing device controls the push piece mechanism to prepare a sample smear for the sample to be detected.
In this embodiment, when the numerical values corresponding to the components of the sample to be detected are abnormal, the sample to be detected may be referred to as an abnormal sample; and when a special cell (such as a special lymphocyte) appears in the sample to be detected, or the source of the sample to be detected is a person to be detected corresponding to a special case, or the source department of the sample to be detected is a special department, the sample to be detected is a special sample. It is to be understood that this is by way of illustration only and not by way of limitation.
703 controlling the transport mechanism to transport the sample smear from the sheet pushing mechanism to the staining mechanism.
After the preparation of the sample smear is completed in the push piece staining device, the conveying mechanism is controlled to convey the sample smear to the staining mechanism along a production line.
And 704, controlling the staining mechanism to stain the sample to be measured on the sample smear according to the selected staining parameters of the sample information.
When the slide dyeing device determines that the sample to be tested is a special sample or an abnormal sample according to the sample information, the slide dyeing device controls the dyeing mechanism to determine a first dyeing parameter corresponding to the sample to be tested according to a preset mapping relation table, and the dyeing parameter is used for dyeing the sample to be tested on the sample smear; and if the slide piece dyeing device determines that the sample to be detected is a normal sample according to the sample information, the slide piece dyeing device controls the dyeing mechanism to dye the sample to be detected on the sample smear according to conventional dyeing parameters.
In this embodiment, the preset mapping relationship table is used to indicate a corresponding relationship between the sample and the staining parameter. In one example, the preset mapping relationship table is shown in the following table 1:
TABLE 1
| Sample information | Dyeing time | Ratio of dye liquor to buffer |
| Body fluid | ||
| 2 minutes | 1：18 | |
| High leukocyte sample | 8 minutes | 1：6 |
| Low leukocyte sample | 1 minute | 1：18 |
The dyeing time and the proportion of the dye solution and the buffer solution can be set according to the requirements, and the slide pushing and dyeing device automatically selects the required dyeing parameters to dye according to the sample information. The above staining protocol is only one example, and may actually be used to classify more cells, for example, setting multiple staining parameters according to different leukocyte value ranges.
In this application embodiment, the staining device for the push piece specifically stains the sample to be tested on the sample smear according to the first staining parameter includes the following possible implementation manners:
in a possible implementation manner, the push piece dyeing device controls the push piece mechanism to push out a plurality of sample smears, at the moment, the push piece dyeing device controls the dyeing mechanism to dye at least one sample smear according to the first dyeing parameter, and the rest sample smears are dyed by adopting a conventional dyeing parameter. For example, the sheet pushing mechanism pushes out three sample smears, namely A, B and C, on the sample to be detected; the smear dyeing apparatus selects a sample smear a among them to be dyed according to the first dyeing parameter, and the remaining sample smear B and sample smear C to be dyed according to the conventional dyeing parameter.
In another implementation manner, after determining a first dyeing parameter of the sample to be tested, the slide pushing and dyeing device adjusts the first dyeing parameter within a preset range to obtain a dyeing parameter set; and then controlling the staining mechanism to stain any one of the sample smears according to the first staining parameter, and controlling the staining mechanism to stain the rest sample smears according to each group of staining parameters in the staining parameter set. For example, the first staining parameter of the sample to be detected is: the staining time was 2 minutes, the ratio of staining solution to buffer was 1: 18, the temperature of the dye solution and the buffer solution is 30 ℃. In an exemplary embodiment, the set of staining parameters comprises: staining parameter a (staining time of 2 minutes, ratio of staining solution to buffer solution of 1: 16, temperature of staining solution to buffer solution of 32 ℃), staining parameter B (staining time of 1 minute, ratio of staining solution to buffer solution of 1: 16, temperature of staining solution to buffer solution of 31 ℃), staining parameter C (staining time of 1 minute, ratio of staining solution to buffer solution of 1: 18, temperature of staining solution to buffer solution of 35 ℃). The sheet pushing mechanism pushes out four sample smears, namely A, B, C and D; the slide-pushing staining device selects the sample smear A to stain according to the first staining parameter; staining the sample smear B according to the staining parameter A; staining the sample smear C according to the staining parameter B; the sample smear D was stained according to staining parameter C.
Specifically, referring to fig. 8, an embodiment of the staining method for staining a sample according to the embodiment of the present application includes:
801, obtaining sample information of a sample to be detected.
The dyeing device receives the sample information of the sample to be detected sent by the sample analyzer or directly obtains the sample information of the sample to be detected input by a user through input equipment.
In an embodiment of the present application, the sample information includes at least one item of information of each component in the sample to be tested, a sample type of the sample to be tested, source information of the sample to be tested, and application scenario information of the sample to be tested. The information on each component (which may be referred to as a cell detection parameter) in the sample includes hemoglobin information, leukocyte information, and platelet information. The cell detection parameter information can be used for indicating that the corresponding numerical value of the cell detection parameter is abnormal or normal. In this embodiment, the numerical value abnormality corresponding to the cell detection parameter mainly indicates that the numerical value corresponding to the cell detection parameter does not satisfy the corresponding threshold (for example, is greater than the threshold or is not within the threshold range), the cell detection parameter is default (for example, the cell detection parameter is empty or the numerical value corresponding to the cell detection parameter is an initial value), the numerical value corresponding to the cell detection parameter directly indicates whether there is an abnormality (for example, it is not required to determine by a threshold method), and the like all of which are considered as the numerical value abnormality corresponding to the cell detection parameter. Otherwise, the corresponding numerical value of the cell detection parameter is normal.
And the sample type of the sample to be tested is used for indicating that the sample to be tested is blood, body fluid or marrow fluid.
The source information of the sample to be detected comprises information of a person to be detected corresponding to the sample to be detected or a source department of the sample to be detected. For example, the historical case of the person to be detected shows that the person to be detected has leukemia; or the sample to be detected is from a specific department, for example, the detection result information is from but not limited to the procreation medical department, and the specific department may be determined according to medical needs, which is not limited in this embodiment.
The application scene information of the sample comprises a detection item corresponding to the sample. Such as the detection of only leukocytes or only platelets in the test sample, etc. The details are not limited herein.
And 802, controlling the staining mechanism to stain the sample to be measured on the sample smear according to the selected staining parameters of the sample information.
When the dyeing device determines that the sample to be detected is a special sample or an abnormal sample according to the sample information, the dyeing device controls the dyeing mechanism to determine a first dyeing parameter corresponding to the sample to be detected according to a preset mapping relation table, and dyes the sample to be detected on the sample smear by using the dyeing parameter; and if the dyeing device determines that the sample to be detected is a normal sample according to the sample information, the dyeing device controls the dyeing mechanism to dye the sample to be detected on the sample smear according to conventional dyeing parameters.
In this embodiment, the preset mapping relationship table is used to indicate a corresponding relationship between the sample and the staining parameter. In one example, the preset mapping relationship table is shown in the following table 2:
TABLE 2
| Sample information | Dyeing time | Ratio of dye liquor to buffer |
| Body fluid | ||
| 2 minutes | 1：18 | |
| High leukocyte sample | 8 minutes | 1：6 |
| Low leukocyte sample | 1 minute | 1：18 |
The dyeing time and the proportion of the dye solution and the buffer solution can be set according to the requirements, and the dyeing device automatically selects the required dyeing parameters to dye according to the sample information. The above staining protocol is only one example, and may actually be used to classify more cells, for example, setting multiple staining parameters according to different leukocyte value ranges.
In this application embodiment, the staining device specifically stains the sample to be tested on the sample smear according to the first staining parameter, which includes the following possible implementation manners:
in a possible implementation, the staining device is required to stain the plurality of sample smears, when the staining device controls the staining mechanism to stain at least one of the sample smears according to the first staining parameter, and stain the rest of the sample smears according to the conventional staining parameter. For example, the sheet pushing mechanism pushes out three sample smears, namely A, B and C, on the sample to be detected; the staining apparatus selects wherein the sample smear a is stained according to the first staining parameter and the remaining sample smear B and sample smear C are stained according to the conventional staining parameter.
In another implementation manner, after determining a first dyeing parameter of the sample to be tested, the dyeing apparatus adjusts the first dyeing parameter within a preset range to obtain a dyeing parameter set; and then controlling the staining mechanism to stain any one of the sample smears according to the first staining parameter, and controlling the staining mechanism to stain the rest sample smears according to each group of staining parameters in the staining parameter set. For example, the first staining parameter of the sample to be detected is: the staining time was 2 minutes, the ratio of staining solution to buffer was 1: 18, the temperature of the dye solution and the buffer solution is 30 ℃. In an exemplary embodiment, the set of staining parameters comprises: staining parameter a (staining time of 2 minutes, ratio of staining solution to buffer solution of 1: 16, temperature of staining solution to buffer solution of 32 ℃), staining parameter B (staining time of 1 minute, ratio of staining solution to buffer solution of 1: 16, temperature of staining solution to buffer solution of 31 ℃), staining parameter C (staining time of 1 minute, ratio of staining solution to buffer solution of 1: 18, temperature of staining solution to buffer solution of 35 ℃). The sheet pushing mechanism pushes out four sample smears, namely A, B, C and D; the staining device selects the sample smear A to stain according to the first staining parameter; staining the sample smear B according to the staining parameter A; staining the sample smear C according to the staining parameter B; the sample smear D was stained according to staining parameter C.
The sample dyeing process in the embodiment of the present application is described below by taking a slide dyeing apparatus as an example:
specifically, referring to fig. 9, a dyeing process of the slide dyeing apparatus includes:
the sample analyzer performs sample analysis test on a sample to be tested to obtain sample information; the sample analyzer sends the sample information to a slide-pushing dyeing device; the slide-pushing dyeing device judges whether the sample to be detected is a special sample according to the sample information; if so, the control mechanism controls the sheet pushing mechanism to push two sample smears, and the control mechanism controls the dyeing mechanism to dye one sample smear according to a conventional dyeing parameter and dye the other sample smear according to a first dyeing parameter corresponding to the sample to be detected; if not, the control mechanism controls the smear pushing mechanism to push out a sample smear, and the sample smear is dyed according to the conventional dyeing parameters.
Specifically referring to fig. 10, another dyeing process of the slide dyeing apparatus includes:
the sample analyzer performs sample analysis test on a sample to be tested to obtain sample information; the sample analyzer sends the sample information to a slide-pushing dyeing device; the slide-pushing dyeing device judges whether the sample to be detected is a special sample according to the sample information; if so, the control mechanism controls the sheet pushing mechanism to push a sample smear, and the control mechanism controls the dyeing mechanism to dye according to a first dyeing parameter corresponding to the sample to be detected; if not, the control mechanism controls the smear pushing mechanism to push out a sample smear, and the sample smear is dyed according to the conventional dyeing parameters.
It is understood that in the present embodiment, dyeing implementation schemes include drop dyeing, single-piece exhaust dyeing, and multi-piece vat dyeing. In the case of the drop-staining protocol, a mixture of the staining solution and buffer solution is dropped onto the sample smear. When continuous dyeing is carried out, the length of the dyeing time and the proportion of the dye liquor buffer solution in the mixed liquor can be changed at any time according to the requirements. For the single-piece dip dyeing scheme, a sample smear is put into a dyeing box, and corresponding dye liquor or mixed liquor is added into the dyeing box for dyeing, so that the length of the dyeing time and the proportion of the dye liquor buffer solution in the mixed liquor can be changed at any time as required as in drop dyeing. For the multi-vat staining scheme, a plurality of sample smears are placed in one vat for staining, so that the proportion of a staining solution and a buffer solution cannot be adjusted at any time in a mixed solution staining process in a continuous staining process. If the proportion of the dye liquor and the buffer liquor needs to be changed, the proportion can be changed and the next piece of dye liquor can be dyed after the dyeing in the process is finished, and the time for changing the dye liquor is long and the consumed time is long. Therefore, the multi-piece vat dyeing is not suitable for adjusting the proportion of the dye solution and the buffer solution in the mixed solution, and only can adjust the dyeing time.
In the embodiment of the present application, the slide dyeing apparatus is as shown in fig. 3, and details thereof are omitted. The dyeing device has all the functions of the dyeing mechanism shown in fig. 3, and the details are not repeated here.
An embodiment of the present application further provides a sample analysis system, specifically please refer to fig. 11, where the sample analysis system includes a sample analysis device 1101, configured to detect a sample to be detected and obtain sample information;
the slide pushing and dyeing device 1102 comprises a slide pushing mechanism and a dyeing mechanism, wherein the slide pushing mechanism is used for preparing a sample smear, and the dyeing mechanism is used for dyeing a sample to be detected on the sample smear;
a cell image analysis device 1103 configured to photograph and analyze a sample smear;
a transport device 1104 comprising a first transport track for transporting a sample from the sample analysis device to the blade pushing mechanism, a second transport track for transporting a sample smear from the blade pushing mechanism to the staining mechanism, and a third transport track for transporting a sample smear from the staining mechanism to the cytoimage analysis device; and
a control mechanism 1105 communicatively connected to the sample analyzer 1101, the push sheet mechanism, the staining mechanism, the cell image analyzer 1103, and the transport device 1104, and configured to:
obtaining sample information of a sample from the sample analysis device 1101; controlling the first conveying track to convey the sample to be detected to the sheet pushing mechanism; controlling the sheet pushing mechanism to prepare a sample smear and controlling the second conveying track to convey the sample smear to the dyeing mechanism; controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear; and controlling the third conveying track to convey the sample smear from the dyeing mechanism to the cell image analysis device 1103 for shooting and analysis.
It is clear to those skilled in the art that, for convenience and brevity of description, the specific working processes of the above-described systems, apparatuses and units may refer to the corresponding processes in the foregoing method embodiments, and are not described herein again.
In the several embodiments provided in the present application, it should be understood that the disclosed system, apparatus and method may be implemented in other manners. For example, the above-described apparatus embodiments are merely illustrative, and for example, the division of the units is only one logical division, and other divisions may be realized in practice, for example, a plurality of units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection through some interfaces, devices or units, and may be in an electrical, mechanical or other form.
The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.
In addition, functional units in the embodiments of the present application may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit. The integrated unit can be realized in a form of hardware, and can also be realized in a form of a software functional unit.
The integrated unit, if implemented in the form of a software functional unit and sold or used as a stand-alone product, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present application may be substantially implemented or contributed to by the prior art, or all or part of the technical solution may be embodied in a software product, which is stored in a storage medium and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present application. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk or an optical disk, and other various media capable of storing program codes.
The above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.
Claims (21)
1. A staining method for sample staining is applied to a slide staining device, and the slide staining device comprises a slide pushing mechanism, a slide fixing mechanism and a staining mechanism, wherein the slide pushing mechanism is used for smearing a sample to be detected on a slide to prepare a sample smear; the staining mechanism is used for staining the sample to be measured on the sample smear, and the conveying mechanism is used for conveying the sample smear from the push sheet mechanism to the staining mechanism, and the staining mechanism is characterized by comprising:
obtaining sample information of the sample to be detected;
controlling the sheet pushing mechanism to prepare a sample smear according to the sample information;
controlling the transport mechanism to transport the sample smear from the sheet pushing mechanism to the staining mechanism;
and controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear.
2. The method of claim 1, wherein the controlling the staining mechanism to select a staining parameter for staining the sample to be tested on the sample smear according to the sample information comprises:
if the sample information indicates that the sample to be detected is a special sample, controlling the staining mechanism to stain the sample to be detected on the sample smear according to a first staining parameter, wherein the first staining parameter is determined according to a preset mapping relation table, and the preset mapping relation table is used for indicating the corresponding relation between the sample and the staining parameter;
and if the sample information indicates that the sample to be detected is a conventional sample, controlling the staining mechanism to stain the sample to be detected on the sample smear according to a conventional staining parameter, wherein the conventional staining parameter is a staining parameter preconfigured by the slide pushing and staining device.
3. The method of any one of claims 1-2, wherein the controlling the blade pushing mechanism to prepare a sample smear according to the sample information comprises:
if the sample information indicates that the sample to be detected is a special sample, controlling the sheet pushing mechanism to prepare at least one sample smear;
and if the sample information indicates that the sample to be detected is a conventional sample, controlling the sheet pushing mechanism to prepare a sample smear.
4. The method of claim 3, wherein the controlling the staining mechanism to select a staining parameter for staining the sample to be tested on the sample smear according to the sample information comprises:
if the sample information indicates that the sample to be detected is a special sample, controlling the staining mechanism to stain the sample on any selected sample smear in the at least one sample smear according to a first staining parameter, wherein the first staining parameter is determined according to a preset mapping relation table, and the preset mapping relation table is used for indicating the corresponding relation between the sample and the staining parameter; staining samples on other of the sample smears according to the conventional staining parameter, wherein the conventional staining parameter is a staining parameter pre-configured by the slide staining device.
5. The method of claim 3, wherein the controlling the staining mechanism to select a staining parameter for staining the sample to be tested on the sample smear according to the sample information comprises:
adjusting a first dyeing parameter within a preset range to obtain a dyeing parameter set, wherein the dyeing parameter set comprises a plurality of groups of dyeing parameters, the first dyeing parameter is determined according to a preset mapping relation table, and the preset mapping relation table is used for indicating the corresponding relation between a sample and the dyeing parameters;
and controlling the staining mechanism to stain the sample on any selected one of the at least one sample smear according to the first staining parameter, and stain the sample on other sample smears in the sample smear according to each group of staining parameters in the staining parameter set.
6. The method according to any one of claims 1 to 5, wherein the obtaining of the sample information of the sample to be tested comprises:
acquiring the sample information sent by a sample analyzer, wherein the sample information is obtained by analyzing by the sample analyzer;
or the like, or, alternatively,
receiving the sample information input through an input device.
7. The method according to any one of claims 1 to 6, wherein the sample information includes at least one of composition information of the sample to be tested, a sample type of the sample to be tested, source information of the sample to be tested, and application scenario information of the sample to be tested.
8. The method of any one of claims 1 to 7, wherein the staining parameters include staining time, staining solution to buffer ratio, and temperature.
9. The method of any one of claims 1 to 8, wherein the test sample comprises blood, body fluids and bone marrow fluids.
10. A staining method for staining a sample, applied to a staining apparatus including a staining mechanism for staining a sample to be measured on a sample smear, comprising:
obtaining sample information of a sample to be detected;
and controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear.
11. The method of claim 10, wherein the controlling the staining mechanism to select a staining parameter for staining the sample to be tested on a sample smear according to sample information comprises:
if the sample information indicates that the sample to be detected is a special sample, controlling the staining mechanism to stain the sample to be detected on the sample smear according to a first staining parameter, wherein the first staining parameter is determined according to the preset mapping relation table, and the preset mapping relation table is used for indicating the corresponding relation between the sample and the staining parameter;
and if the sample information indicates that the sample to be detected is a conventional sample, controlling the staining mechanism to stain the sample to be detected on the sample smear according to a conventional staining parameter, wherein the conventional staining parameter is a staining parameter pre-configured by the staining device.
12. The method of any one of claims 10 to 11, wherein the controlling the staining mechanism to stain the sample to be tested on a sample smear according to the sample information selecting a staining parameter comprises:
if the sample information indicates that the sample to be detected is a special sample and the number of the sample smears is at least one, controlling the staining mechanism to stain the sample on any selected sample smear in the at least one sample smear according to a first staining parameter, wherein the first staining parameter is determined according to a preset mapping relation table which is used for indicating the corresponding relation between the sample and the staining parameter; staining the samples on other of the sample smears according to a conventional staining parameter, the conventional staining parameter being a staining parameter preconfigured by the staining device.
13. The method of any one of claims 10 to 11, wherein the controlling the staining mechanism to stain the sample to be tested on a sample smear according to the sample information selecting a staining parameter comprises:
if the sample information indicates that the sample to be detected is a special sample and the number of the sample smears is at least one, controlling the staining mechanism to stain the sample on any selected sample smear in the at least one sample smear according to a first staining parameter, wherein the first staining parameter is determined according to a preset mapping relation table, and the preset mapping relation table is used for indicating the corresponding relation between the sample and the staining parameters;
and adjusting the first staining parameter within a preset range to obtain a staining parameter set, wherein the staining parameter set comprises a plurality of groups of staining parameters, and the staining mechanism is controlled to stain the samples on other sample smears in the sample smears according to each group of staining parameters in the staining parameter set.
14. The method according to any one of claims 10 to 13, wherein the obtaining of the sample information of the sample to be tested comprises:
acquiring the sample information sent by a sample analyzer, wherein the sample information is obtained by analyzing by the sample analyzer;
or the like, or, alternatively,
receiving the sample information input through an input device.
15. The method according to any one of claims 10 to 14, wherein the sample information includes at least one of information on each component in the sample, a sample type of the sample, source information of the sample, and application scenario information of the sample.
16. The method of any one of claims 10 to 15, wherein the staining parameters include staining time, staining solution to buffer ratio, and temperature.
17. The method of any one of claims 10 to 16, wherein the test sample comprises blood, body fluids and bone marrow fluids.
18. A push-piece dyeing device is characterized by comprising:
the slide pushing mechanism is used for smearing the sample to be detected on the slide to prepare a sample smear;
a staining mechanism for staining a sample on the sample smear, the staining mechanism having a special staining mode and a general staining mode;
a transport mechanism for transporting the sample smear to the staining mechanism;
a control mechanism in communicative connection with the blade pushing mechanism, the dyeing mechanism, and the conveying mechanism, configured to: determining an operation mode of the dyeing mechanism from a special dyeing mode and a common dyeing mode, controlling the dyeing mechanism to dye a sample to be detected on the sample smear according to a first dyeing parameter when the operation mode is determined to be the special dyeing mode, determining the first dyeing parameter according to a preset mapping relation table, wherein the preset mapping relation table is used for indicating the corresponding relation between the sample and the dyeing parameter, and controlling the dyeing mechanism to dye the sample to be detected on the sample smear according to a conventional dyeing parameter when the operation mode is determined to be the common dyeing mode, wherein the conventional dyeing parameter is a dyeing parameter preconfigured by the slide-pushing dyeing device.
19. The apparatus of claim 18, wherein the film pushing mechanism further comprises a special film making mode and a regular film making mode, the control mechanism further configured to: and determining the operation mode of the film pushing mechanism from a special film making mode and a conventional film making mode, controlling the film pushing mechanism to prepare at least one sample smear when the operation mode is determined to be the special film making mode, and controlling the film pushing mechanism to prepare one sample smear when the operation mode is determined to be the conventional film making mode.
20. A sample staining apparatus, comprising:
a staining mechanism for staining a sample on a sample smear, the staining mechanism having a special staining mode and a general staining mode;
a control mechanism, communicatively coupled to the dyeing mechanism, configured to: determining an operation mode of the staining mechanism from a special staining mode and a common staining mode, controlling the staining mechanism to stain a sample to be detected on the sample smear according to a first staining parameter when the operation mode is determined to be the special staining mode, determining the first staining parameter according to the preset mapping relation table, wherein the preset mapping relation table is used for indicating the corresponding relation between the sample and the staining parameter, and controlling the staining mechanism to stain the sample to be detected on the sample smear according to a conventional staining parameter when the operation mode is determined to be the common staining mode, wherein the conventional staining parameter is a staining parameter preconfigured by the staining device.
21. A sample analysis system, comprising:
the sample analysis device is used for detecting a sample to be detected and obtaining sample information;
the push piece dyeing device comprises a push piece mechanism and a dyeing mechanism, wherein the push piece mechanism is used for preparing a sample smear, and the dyeing mechanism is used for dyeing a sample to be detected on the sample smear;
the cell image analysis device is used for shooting and analyzing the sample smear;
a transport device comprising a first transport track for transporting a sample from the sample analysis device to the blade pushing mechanism, a second transport track for transporting a sample smear from the blade pushing mechanism to the staining mechanism, and a third transport track for transporting a sample smear from the staining mechanism to the cellular image analysis device; and
a control mechanism communicatively coupled to the sample analysis device, the blade pushing mechanism, the staining mechanism, the cell image analysis device, and the transport device and configured to:
obtaining sample information of a sample from the sample analysis device;
controlling the first conveying track to convey the sample to be detected to the sheet pushing mechanism; controlling the sheet pushing mechanism to prepare a sample smear and controlling the second conveying track to convey the sample smear to the dyeing mechanism; controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear; and controlling the third conveying track to convey the sample smear from the dyeing mechanism to the cell image analysis device for shooting and analysis.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116413111A (en) * | 2023-06-08 | 2023-07-11 | 深圳市森盈生物科技有限公司 | Intelligent dyeing control method and intelligent dyeing double-hole machine |
| CN117147266A (en) * | 2023-10-31 | 2023-12-01 | 广州医科大学附属第一医院（广州呼吸中心） | Blood disease analysis system based on blood morphology special dyeing |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116413111A (en) * | 2023-06-08 | 2023-07-11 | 深圳市森盈生物科技有限公司 | Intelligent dyeing control method and intelligent dyeing double-hole machine |
| CN116413111B (en) * | 2023-06-08 | 2023-08-11 | 深圳市森盈生物科技有限公司 | Intelligent dyeing control method and intelligent dyeing double-hole machine |
| CN117147266A (en) * | 2023-10-31 | 2023-12-01 | 广州医科大学附属第一医院（广州呼吸中心） | Blood disease analysis system based on blood morphology special dyeing |
| CN117147266B (en) * | 2023-10-31 | 2024-02-13 | 广州医科大学附属第一医院（广州呼吸中心） | Blood disease analysis system based on blood morphology special dyeing |
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