CN113917083A - Dinotefuran drug resistance test method - Google Patents
Dinotefuran drug resistance test method Download PDFInfo
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- CN113917083A CN113917083A CN202111199548.XA CN202111199548A CN113917083A CN 113917083 A CN113917083 A CN 113917083A CN 202111199548 A CN202111199548 A CN 202111199548A CN 113917083 A CN113917083 A CN 113917083A
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- YKBZOVFACRVRJN-UHFFFAOYSA-N dinotefuran Chemical compound [O-][N+](=O)\N=C(/NC)NCC1CCOC1 YKBZOVFACRVRJN-UHFFFAOYSA-N 0.000 title claims abstract description 46
- 206010059866 Drug resistance Diseases 0.000 title claims abstract description 23
- 238000010998 test method Methods 0.000 title claims description 14
- 238000012360 testing method Methods 0.000 claims abstract description 132
- 238000003756 stirring Methods 0.000 claims abstract description 65
- 239000007788 liquid Substances 0.000 claims abstract description 57
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 45
- 238000009434 installation Methods 0.000 claims description 17
- 229920000742 Cotton Polymers 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 9
- 230000000903 blocking effect Effects 0.000 claims description 8
- 238000004891 communication Methods 0.000 claims description 7
- 230000000994 depressogenic effect Effects 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 3
- 238000013461 design Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 238000013019 agitation Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241001124076 Aphididae Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001466042 Fulgoromorpha Species 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 229940127021 low-dose drug Drugs 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a dinotefuran drug resistance testing method which comprises a testing box, wherein a stirring chamber and a testing chamber are arranged in the testing box, a liquid inlet pipe and a liquid outlet pipe are arranged on the testing box, the liquid inlet pipe is positioned on the side wall of the stirring chamber and is communicated with the liquid inlet pipe, and the liquid outlet pipe is positioned at the lower end of the testing chamber. The upper end of the test box is also provided with a second motor, the lower end of the second motor is provided with a stirring rod, the stirring rod is arranged in the test chamber, and the stirring rod is provided with a plurality of stirring blades. A fixing frame is arranged between the stirring chamber and the testing chamber, and a plurality of rotating plates are arranged in the middle of the fixing frame. The dinotefuran drug resistance testing method is high in practicability, and can be used for testing various different types and different ages of pests to obtain multiple groups of testing data. The adopted test box is compact in structure and ingenious in design, the dinotefuran solution smell is generated by the stirring chamber, and the dinotefuran solution enters the test chamber for testing, so that the test efficiency is improved, the expected test effect is achieved, and the drug resistance test of dinotefuran is realized.
Description
Technical Field
The invention relates to the technical field of dinotefuran test, in particular to a method for testing the drug resistance of dinotefuran.
Background
Dinotefuran is a pesticide, mainly plays a role in killing aphids, plant hoppers, whiteflies, thrips and other pests, and is widely applied to crops such as wheat, rice, cotton, vegetables and the like. Drug resistance refers to the ability of an organism to develop immunity to a substance in the body, so that the organism cannot harm itself. The generation of drug resistance is adaptive change after the pathogen is contacted with low-dose drugs for a long time, and the generation of the pathogen is a mechanism for generating the drug resistance of the pathogen by generating enzymes for inactivating the drugs, changing the permeability of membranes to block the entry of the drugs, changing a target structure or changing the original metabolic process.
However, the resistance of pests to pesticides is immune over time and also resistant to pesticides in accordance with the content of the drugs, so that a method capable of testing the resistance of pesticides is required.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a dinotefuran drug resistance testing method.
The technical scheme of the invention is realized as follows:
a dinotefuran drug resistance test method is characterized by comprising the following steps:
s1: collecting different types and different ages of pests, putting the pests into a test chamber in a test box in batches, and making records;
s2: proportioning dinotefuran solutions with different concentrations, injecting the dinotefuran solutions from a liquid inlet pipe, enabling the dinotefuran solutions to flow into a stirring chamber, and recording the concentration of the injected solutions;
s3: starting the first cylinder, the first motor, the second cylinder, the second motor and the third cylinder to enable solution liquid and smell in the stirring chamber to enter the testing chamber, recording testing time, and observing whether living pests exist;
s4: keeping the remaining living pests in the S3 for cultivation, continuously putting the pests into a test chamber after the pests are raised for one week, increasing the concentration of the solution in the S2, continuously recording the test time, and observing whether the pests exist or not; wherein the content of the first and second substances,
the inside of test box has stirring room and test room, feed liquor pipe and fluid-discharge tube have on the test box, the feed liquor pipe is located the lateral wall of stirring room and communicates with the feed liquor pipe, the fluid-discharge tube is located the lower extreme of test room, first cylinder has on the test box, first cylinder lower extreme has first cylinder pole, first cylinder pole extends to the inside of stirring room, the lower extreme of first cylinder pole has the mounting, first motor has on the mounting, the last rotation axis that has of first motor, the rotation axis is located the centre of mounting, the rotation axis has rotatory roller, the cover has the rubber tape on the rotatory roller, the surface of rubber tape has many spoons, the inside fretwork of spooning spoon, the last cross slot that has of spooning, cross slot and the inside intercommunication of spooning spoon, the rotatory roller has an installation breach, the fixed block has in the installation breach, the inside of installation breach has the fixed part, the fixed block passes through the bolt and installs on the fixed part, the both sides of fixed part have the installation clearance, the both ends of rubber tape are arranged in the installation clearance of fixed part both sides respectively, the both sides of fixed block have the bellying, the depressed part has in the installation breach, what the bellying matches arranges in the depressed part, an arc has on the inner wall of stirring room, the vertical plate has on the arc, the vertical plate is located the upper end of rotatory roller.
In the present invention, the lower end of the test box has a third cylinder located at the lower end of the test chamber, and the third cylinder has a push piston thereon, which is disposed inside the stirring chamber.
In the present invention, the rotating roller has a plurality of chenille bars symmetrically arranged on both side surfaces thereof.
In the present invention, the bolt has a plurality of bolts each having a different length, and the bolt has a driving portion.
In the invention, the upper end of the test box is also provided with a second motor, the lower end of the second motor is provided with a stirring rod, the stirring rod is arranged in the test chamber, and the stirring rod is provided with a plurality of stirring blades.
In the invention, two sides of the fixed block are also provided with first curved surface parts, the mounting notch is provided with a second curved surface part, and the first curved surface part and the second curved surface part are mounted in a matched mode.
In the invention, the end face of the rotating plate facing the test chamber is provided with a supporting part and medical absorbent cotton, and the middle of the medical absorbent cotton is positioned on the supporting part.
In the invention, a communicating groove is formed in the test box and used for communicating the stirring chamber with the test chamber, a sealing plate is arranged at the upper end of the communicating groove, a rotating rod is arranged in the middle of the sealing plate, a rotating hand wheel is arranged at one end of the rotating rod, and a cross rod is arranged in the middle of the rotating hand wheel.
In the invention, an inclined block is arranged between the fixed frame and the communication groove, and the inclined block inclines from the direction of the fixed frame to the direction of the sealing plate.
In the invention, a fixing frame is arranged between the stirring chamber and the testing chamber, a plurality of rotating plates are arranged in the middle of the fixing frame and are connected in an ending way, the rotating plates are installed in the middle of the fixing frame through a rotating rod, one end of the rotating rod extends to the outside of the testing box, a gear is arranged on the rotating rod and is arranged outside the testing box, a fixing seat is arranged at the upper end of the testing box, a second air cylinder is arranged on the fixing seat, a rack is arranged at the lower end of the second air cylinder and is in meshed connection with the gear, a first blocking part is arranged at the upper end of the rotating plate, and a second blocking part is arranged at the lower end of the rotating plate.
The dinotefuran drug resistance testing method has the following beneficial effects: the dinotefuran drug resistance testing method is high in practicability, and can be used for testing various different types and different ages of pests to obtain multiple groups of testing data. The adopted test box is compact in structure and ingenious in design, the dinotefuran solution smell is generated by the stirring chamber, and the dinotefuran solution enters the test chamber for testing, so that the test efficiency is improved, the expected test effect is achieved, and the drug resistance test of dinotefuran is realized.
Drawings
FIG. 1 is a schematic view of the structure of the test box of the present invention;
FIG. 2 is another schematic view of the structure of FIG. 1;
FIG. 3 is a top view of FIG. 1;
FIG. 4 is a cross-sectional view taken along line A-A of FIG. 3;
FIG. 5 is a partial enlarged view of FIG. 4 at B;
FIG. 6 is a schematic view of the internal structure of FIG. 1;
FIG. 7 is another schematic view of the structure of FIG. 6;
FIG. 8 is a schematic view of the arcuate member, first cylinder and rotating roller configuration of FIG. 6;
FIG. 9 is an exploded view of the rotating roller, bolts and mounting block of FIG. 8;
FIG. 10 is a schematic view of the rubber belt structure of FIG. 6;
FIG. 11 is a schematic view of the seal plate of FIG. 6;
FIG. 12 is a schematic view showing the construction of the fixed frame, the second cylinder and the tilting block of FIG. 6;
FIG. 13 is a schematic view of the rotating plate of FIG. 12;
FIG. 14 is a flow chart of the steps performed in the present invention.
In the figure: the test box 1, the stirring chamber 2, the test chamber 3, the liquid inlet pipe 4, the liquid outlet pipe 5, the first cylinder 6, the fixing member 7, the first motor 8, the rotating shaft 9, the rotating roller 10, the rubber belt 11, the notch 111, the scoop 12, the lateral groove 13, the mounting notch 14, the fixing block 15, the fixing portion 16, the bolt 17, the mounting gap 18, the protruding portion 19, the recessed portion 20, the arc member 21, the vertical plate 22, the second motor 23, the stirring rod 24, the stirring blade 25, the fixing frame 26, the rotating plate 27, the rotating rod 28, the gear 29, the fixing seat 30, the second cylinder 31, the rack 32, the first blocking portion 33, the second blocking portion 34, the third cylinder 35, the pushing piston 36, the chenille bar 37, the screwing portion 38, the first curved surface portion 39, the second curved surface portion 40, the supporting portion 41, the medical absorbent cotton 42, the rotating rod communicating groove 43, the sealing plate 44, the sealing plate 45, the rotating hand wheel 46, the cross bar 47, the inclined block 48, the rubber belt 11, the fixing block, the inclined block 48, the inclined block, and the fixing plate, A filter plate 49 and a filter screen 50.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
As shown in FIGS. 1 to 14, the method for testing the dinotefuran drug resistance comprises the following steps:
s1: and (3) putting pests, collecting pests of different types and different ages, putting the pests into a test chamber in a test box in batches, and making records. When the pest insects are put in, the same kind of pests in the same age period and/or the same kind of pests in different age periods and/or different kinds of pests in the same age period and/or different kinds of pests in different age periods can be put in a test. The test diversification is realized, and more accurate test data is obtained.
S2: proportioning dinotefuran solution, proportioning dinotefuran solutions with different concentrations, injecting the dinotefuran solution from a liquid inlet pipe, allowing the dinotefuran solution to flow into a stirring chamber, and recording the concentration of the injected solution.
S3: and starting equipment, starting the first cylinder, the first motor, the second cylinder, the second motor and the third cylinder, enabling the solution liquid and the smell in the stirring chamber to enter the testing chamber, recording the testing time, and observing whether living pests exist. If both die, the next batch is placed for further testing to see if there are any surviving pests.
S4: and observing whether the pests survive or not, keeping the remaining pests alive in the S3 for cultivation, continuously putting the pests into the test chamber after the pests are raised for one week, increasing the concentration of the solution in the S2, continuously recording the test time, and observing whether the pests survive or not. If repeated rearing of pests with survival is observed, the dosage is increased once after each time of pest survival, and then the test observation is carried out until the pests die.
To be able to express the test method more clearly, the following table shows:
wherein A represents a pest, B represents another pest, and + represents an adult pest, and-represents a non-adult pest. M represents the dinotefuran solution, while 1, 2, 3, 4 represent different concentrations of the solution. However, in the actual test, a plurality of pest tests can be added, and the test is not limited to two, and the solution concentration can be configured into a plurality of different concentrations.
Therein, the test chamber 1 has therein an agitation chamber 2 and a test chamber 3. The stirring chamber 2 is driven by the rotation of the first motor 8 to rotate the rotating roller 10, so that the chenille sticks 37 on two sides of the rotating roller 10 are scattered on the edge under the action of centrifugal force, then the third cylinder 35 is started, the pushing piston 36 is pushed, the liquid level is raised, or the first cylinder 6 pushes the fixing piece 7 to descend, so that the chenille sticks 37 are stained with liquid, because the water absorption of the chenille sticks 37 is better, so that more solution can be absorbed, then the chenille sticks out, so that the solution is volatilized, dinotefuran solution smell exists in the test box, and the dinotefuran solution smell enters the test chamber from the stirring chamber. And the scoop 12 also enters the liquid, then the liquid can be driven from the stirring chamber 2 through the chenille strips 37 and the scoop 12 to splash onto the arc 21, and then the chenille strips 37 are blocked by the vertical plate 22 to hit the liquid at the upper end of the chenille strips 37 to the vertical plate 22, so that most of the liquid hits the vertical plate 22 and falls down. The test chamber 3 is agitated by the agitating blades 25 for remixing the transferred liquid in the agitating chamber 2.
The test box 1 is provided with a liquid inlet pipe 4 and a liquid outlet pipe 5, the liquid inlet pipe 4 is provided with a plurality of pipes, water and the dinotefuran solution can be added simultaneously for mixing, and the mixed dinotefuran solution can also be added. And the drain pipe 5 is provided with a place for draining the liquid that enters the test chamber. The liquid inlet pipe 4 is positioned on the side wall of the stirring chamber 2 and is communicated with the liquid inlet pipe 4, and the liquid outlet pipe 5 is positioned at the lower end of the testing chamber 3.
The lower end of the test chamber 1 has a third cylinder 35, the third cylinder 35 being located at the lower end of the agitating chamber 2, the third cylinder 35 having a pushing piston 36 thereon, the pushing piston 36 being disposed inside the agitating chamber 2. When the liquid in the stirring chamber 2 needs to be transferred into the testing chamber 3, the third cylinder 35 is firstly started, so that the pushing piston 36 moves upwards, the pushing piston 36 pushes the liquid in the stirring chamber 2, the first cylinder 6 pushes the rotating roller 10 downwards at the moment, the chenille bars 37 do not collide with the vertical plate 22 when rotating, the rotating roller 10 rotates continuously, the liquid on the chenille bars 37 is thrown onto the rotating plate 27 through centrifugal force, and the liquid can enter the testing chamber 3 from gaps between the rotating plates 27 and the communication groove 43.
Meanwhile, a transparent door which can be opened is arranged on one side surface of the test chamber 3 of the test box 1, and the inside of the test chamber 3 is observed and pests are placed in the transparent door. A transparent observation window may also be provided at the front face of the agitation chamber 2 of the test chamber 1 to facilitate observation of the conditions in the agitation chamber 2.
The test box 1 is provided with a first cylinder 6, and the lower end of the first cylinder 6 is provided with a first cylinder rod. The first cylinder rod extends to the inside of the stirring chamber 2, the lower end of the first cylinder rod is provided with a fixed piece 7, the fixed piece 7 is provided with a first motor 8, the first motor 8 is provided with a rotating shaft 9, and the rotating shaft 9 is positioned in the middle of the fixed piece 7. Have rotatory roller 10 on rotation axis 9, the cover has rubber tape 11 on rotatory roller 10, and the surface of rubber tape 11 has many places spoonfulsion 12, and spoonfulsion 12 is inside fretwork, has a transverse groove 13 on the spoonfulsion 12, and transverse groove 13 and spoonfulsion 12 are inside to be linked together. After the rotating roller 10 rotates, the scoop 12 drives the dinotefuran solution to rotate, then under the action of the centrifugal force of the rotating roller 10, the liquid leaks out of the transverse groove 13 and is scattered around the rotating roller 10, and therefore the dinotefuran solution is volatilized to enter the test chamber.
When the rubber belt 11 needs to be installed quickly and stably, the rotating roller 10 is provided with an installation notch 14, a fixing block 15 is arranged in the installation notch 14, a fixing part 16 is arranged inside the installation notch 14, the fixing block 15 is installed on the fixing part 16 through a bolt 17, and the bolt 17 is provided with a plurality of bolts, wherein the length of each bolt is different. And the bolt 17 has a driving portion 38. Since the length of the bolts 17 is different from place to place, the last turning angle of the screwing portion 38 on each bolt 17 is not uniform when the bolts 17 are screwed into the fixed positions, so that the screwing portion 38 can push the liquid in the stirring chamber 2 also when the rotating roller 10 is rotated, thereby facilitating the mixing of the liquids.
Both sides of the fixing portion 16 have mounting gaps 18, and both ends of the rubber band 11 are respectively placed in the mounting gaps 18 of both sides of the fixing portion 16. The fixing block 15 has protrusions 19 on both sides thereof, and the mounting notches 14 have recesses 20 therein, the protrusions 19 being fittingly received in the recesses 20. And notches 111 at both ends of the rubber band 11 for the projections 19 to be placed, the notches 111 can also be used for the projections 19 to fix the rubber band 11. The two sides of the fixing block 15 are also provided with a first curved surface part 39, the installation notch 14 is provided with a second curved surface part 40, and the first curved surface part 39 and the second curved surface part 40 are installed in a matching mode. The stirring chamber 2 has an arc-shaped member 21 on the inner wall thereof, and the arc-shaped member 21 has a vertical plate 22 thereon, the vertical plate 22 being located at the upper end of the rotating roll 10. The arc 21 is intended to follow the arc and thus fall back into the stirring chamber 2 when liquid is thrown onto it.
And has a plurality of chenille strips 37 symmetrically arranged on both side surfaces of the rotating roller 10. The chenille strips 37 have good water absorption property, absorb the liquid when rotated into the liquid in the stirring chamber 2, and then throw it out again by centrifugation, thereby mixing the liquids. And also to be able to beat the liquid down after hitting the vertical plate 22. So that a portion of the solution that is thrown off enters the test chamber through the open-close rotating plate 27.
The upper end of the test box 1 is further provided with a second motor 23, the lower end of the second motor 23 is provided with a stirring rod 24, the stirring rod 24 is arranged inside the test chamber 3, the stirring rod 24 is provided with a plurality of stirring blades 25, and the stirring blades 25 can stir pests to make the pests active.
And a filter plate 49 is provided at the lower end of the stirring rod 24 to prevent vermin from escaping from the drain pipe 5. A sieve 50 is also provided in the test chamber 3, the sieve 50 allowing the passage of the solution and the smell while preventing the entry of vermin into the stirring chamber 2, and the sieve 50 is provided at the communication groove 43.
A fixed frame 26 is arranged between the stirring chamber 2 and the testing chamber 3, a plurality of rotating plates 27 are arranged in the middle of the fixed block 15, the rotating plates 27 are connected in an ending way, the rotating plates 27 are installed in the middle of the fixed frame 26 through rotating rods 28, one end of each rotating rod 28 extends to the outside of the testing box 1, a gear 29 is arranged on each rotating rod 28, the gear 29 is arranged on the outside of the testing box 1, a fixed seat 30 is arranged at the upper end of the testing box 1, a second air cylinder 31 is arranged on each fixed seat 30, a rack 32 is arranged at the lower end of each second air cylinder 31, the rack 32 is meshed with the gear 29 and connected with the gear 29, a first blocking part 33 is arranged at the upper end of each rotating plate 27, and a second blocking part 34 is arranged at the lower end of each rotating plate. The end face of the rotary plate 27 facing the test chamber 3 has a support 41 and medical absorbent cotton 42, and the middle of the medical absorbent cotton 42 is placed on the support 41. The solution was blocked by medical absorbent cotton and allowed to drip.
Of course, the uppermost rotary plate 27 is free of the first stopper 33 at its upper end, and the lowermost rotary plate is free of the second stopper 34.
The inside of test box 1 has a communicating groove 43, and communicating groove 43 will stir room 2 and test chamber 3 intercommunication, and the upper end of communicating groove 43 has the closing plate 44, and the centre of closing plate 44 has dwang 45, and the one end of dwang 45 has the rotation hand wheel 46, and the centre of rotation hand wheel 46 has horizontal pole 47, and this horizontal pole 47 is parallel with closing plate 44, when horizontal pole 47 rotated certain angle, alright know the position that closing plate 44 rotated and reached. An inclined block 48 is provided between the fixed frame 26 and the communication groove 43, and the inclined block 48 is inclined from the direction of the fixed frame 26 toward the direction of the seal plate 44.
When a quick test is needed, the sealing plate 44 is only required to be opened, so that the solution falling from the rotating plate 27 can be discharged into the test chamber 3 and then discharged from the liquid discharge pipe 5, the test time is saved, the timeliness is kept, and whether the dinotefuran solution can cause fatal damage to pests in a short time or not is tested.
When it is necessary to transfer the liquid in the agitating chamber 2 to the test chamber 3, the holder is first moved down by the first cylinder 6 so that the chenille strips 37 on the rotating roller 10 are rotated and spread without hitting the vertical plate 22. And the second cylinder 31 is started, so that the second cylinder 31 is pushed downwards, the rack 32 drives the gear 29 to rotate, the upper end of the rotating plate 27 moves into the stirring chamber 2, the lower end of the rotating plate moves into the testing chamber 3, and an inclined state is formed, so that when the liquid on the chenille strip 37 splashes onto the rotating plate 27, the liquid can flow into the testing chamber 3 from the gap between the rotating plate 27 and then drips from the medical absorbent cotton 42. When a part of the liquid does not enter the rotating plate 27, the liquid drops onto the inclined block 48, then moves downward from the inclined block 48, enters the communicating groove 43, and is discharged into the test chamber 3. When the liquid in the stirring chamber 2 is continuously absorbed by the chenille bar 37 and thrown out, the liquid in the stirring chamber 2 is continuously reduced, the liquid level is reduced, at this time, the third cylinder 35 needs to be started, so that the third cylinder 35 pushes the pushing piston 36 to push the liquid, the liquid is pushed, the liquid is raised, and the chenille bar 37 and the ladle 12 can both touch the liquid. During the transfer process, the liquid in the test chamber 3 is ensured not to generate precipitation, and the liquid is ensured to be uniformly in a mixed state. When the liquid in the agitation chamber 2 is completely transferred to the test chamber 3, the liquid is discharged from the drain pipe 5.
Since the first and second stoppers 34 and 35 are provided so that the rotation plate 27 cannot rotate the upper end portion into the stirring chamber 2, it is possible to prevent the liquid from entering the test chamber 3 when the rotation plate 27 is not opened.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A dinotefuran drug resistance test method is characterized by comprising the following steps:
s1: collecting different types and different ages of pests, putting the pests into a test chamber in a test box in batches, and making records;
s2: proportioning dinotefuran solutions with different concentrations, injecting the dinotefuran solutions from a liquid inlet pipe, enabling the dinotefuran solutions to flow into a stirring chamber, and recording the concentration of the injected solutions;
s3: starting the first cylinder, the first motor, the second cylinder, the second motor and the third cylinder to enable solution liquid and smell in the stirring chamber to enter the testing chamber, recording testing time, and observing whether living pests exist;
s4: keeping the remaining living pests in the S3 for cultivation, continuously putting the pests into a test chamber after the pests are raised for one week, increasing the concentration of the solution in the S2, continuously recording the test time, and observing whether the pests exist or not; wherein the content of the first and second substances,
the inside of test box has stirring room and test room, feed liquor pipe and fluid-discharge tube have on the test box, the feed liquor pipe is located the lateral wall of stirring room and communicates with the feed liquor pipe, the fluid-discharge tube is located the lower extreme of test room, first cylinder has on the test box, first cylinder lower extreme has first cylinder pole, first cylinder pole extends to the inside of stirring room, the lower extreme of first cylinder pole has the mounting, first motor has on the mounting, the last rotation axis that has of first motor, the rotation axis is located the centre of mounting, the rotation axis has rotatory roller, the cover has the rubber tape on the rotatory roller, the surface of rubber tape has many spoons, the inside fretwork of spooning spoon, the last cross slot that has of spooning, cross slot and the inside intercommunication of spooning spoon, the rotatory roller has an installation breach, the fixed block has in the installation breach, the inside of installation breach has the fixed part, the fixed block passes through the bolt and installs on the fixed part, the both sides of fixed part have the installation clearance, the both ends of rubber tape are arranged in the installation clearance of fixed part both sides respectively, the both sides of fixed block have the bellying, the depressed part has in the installation breach, what the bellying matches arranges in the depressed part, an arc has on the inner wall of stirring room, the vertical plate has on the arc, the vertical plate is located the upper end of rotatory roller.
2. A dinotefuran resistance test method according to claim 1, wherein the lower end of the test box is provided with a third cylinder, the third cylinder is positioned at the lower end of the test chamber, the third cylinder is provided with a pushing piston, and the pushing piston is arranged inside the stirring chamber.
3. A dinotefuran resistance test method according to claim 1, wherein the rotating roller has a plurality of symmetrically arranged chenille bars on both sides.
4. A dinotefuran drug resistance test method according to claim 1, wherein the bolt is provided with a plurality of bolts, each bolt is different in length, and a screwing part is arranged on each bolt.
5. A dinotefuran drug resistance test method according to claim 1, wherein the upper end of the test box is further provided with a second motor, the lower end of the second motor is provided with a stirring rod, the stirring rod is arranged inside the test chamber, and the stirring rod is provided with a plurality of stirring blades.
6. The dinotefuran drug resistance test method according to claim 1, wherein first curved surface portions are further arranged on two sides of the fixing block, second curved surface portions are arranged in the installation notches, and the first curved surface portions and the second curved surface portions are installed in a matched mode.
7. A dinotefuran drug resistance test method according to claim 1, wherein the end face of the rotating plate facing the test chamber is provided with a support part and medical absorbent cotton, and the middle of the medical absorbent cotton is located on the support part.
8. A dinotefuran drug resistance test method according to claim 1, wherein a communication groove is formed in the test box and communicates the stirring chamber with the test chamber, a sealing plate is arranged at the upper end of the communication groove, a rotating rod is arranged in the middle of the sealing plate, a rotating hand wheel is arranged at one end of the rotating rod, and a cross rod is arranged in the middle of the rotating hand wheel.
9. A dinotefuran drug resistance test method according to claim 8, wherein an inclined block is arranged between the fixing frame and the communication groove, and the inclined block is inclined from the direction of the fixing frame to the direction of the sealing plate.
10. The dinotefuran drug resistance test method according to claim 1, wherein a fixing frame is arranged between the stirring chamber and the test chamber, a plurality of rotating plates are arranged in the middle of the fixing frame, the rotating plates are connected in a tailing manner, the rotating plates are installed in the middle of the fixing frame through rotating rods, one ends of the rotating rods extend to the outside of the test box, gears are arranged on the rotating rods and are arranged outside the test box, a fixing seat is arranged at the upper end of the test box, a second air cylinder is arranged on the fixing seat, a rack is arranged at the lower end of the second air cylinder and is in meshed connection with the gears, a first blocking portion is arranged at the upper end of each rotating plate, and a second blocking portion is arranged at the lower end of each rotating plate.
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| CN202111199548.XA CN113917083A (en) | 2021-10-14 | 2021-10-14 | Dinotefuran drug resistance test method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115166108A (en) * | 2022-06-13 | 2022-10-11 | 深圳市鑫宇环标准技术有限公司 | Method for measuring residual quantity of metrafenone |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115166108A (en) * | 2022-06-13 | 2022-10-11 | 深圳市鑫宇环标准技术有限公司 | Method for measuring residual quantity of metrafenone |
| CN115166108B (en) * | 2022-06-13 | 2023-12-22 | 深圳市鑫宇环标准技术有限公司 | Method for measuring residue quantity of metrafenone |
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