CN113916638B - Kit for cancer gene detection and use method thereof - Google Patents
Kit for cancer gene detection and use method thereof Download PDFInfo
- Publication number
- CN113916638B CN113916638B CN202111176033.8A CN202111176033A CN113916638B CN 113916638 B CN113916638 B CN 113916638B CN 202111176033 A CN202111176033 A CN 202111176033A CN 113916638 B CN113916638 B CN 113916638B
- Authority
- CN
- China
- Prior art keywords
- storage box
- groove
- glass
- groups
- glass placing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108700019961 Neoplasm Genes Proteins 0.000 title claims abstract description 23
- 102000048850 Neoplasm Genes Human genes 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000001514 detection method Methods 0.000 title claims abstract description 14
- 239000011521 glass Substances 0.000 claims abstract description 86
- 238000007789 sealing Methods 0.000 claims abstract description 28
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 230000007246 mechanism Effects 0.000 claims abstract description 6
- 239000003507 refrigerant Substances 0.000 claims abstract description 6
- 230000000149 penetrating effect Effects 0.000 claims abstract description 4
- 230000036285 pathological change Effects 0.000 claims abstract description 3
- 231100000915 pathological change Toxicity 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 238000011282 treatment Methods 0.000 claims description 12
- 206010028980 Neoplasm Diseases 0.000 claims description 8
- 239000012188 paraffin wax Substances 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 7
- 201000011510 cancer Diseases 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 4
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 4
- 230000018044 dehydration Effects 0.000 claims description 2
- 238000006297 dehydration reaction Methods 0.000 claims description 2
- 238000002844 melting Methods 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- 230000001360 synchronised effect Effects 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 8
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 238000004891 communication Methods 0.000 abstract description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000002349 favourable effect Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 229940044683 chemotherapy drug Drugs 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/366—Moulds; Demoulding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of gene detection, in particular to a kit for cancer gene detection and a using method thereof, which are beneficial to reducing the communication area between the outside and the inside of a storage box, improving the tightness of the kit, further improving the accuracy of cancer gene detection, improving the automation degree of the kit and facilitating the respective access control of a plurality of groups of slides; the method comprises the following steps: the storage box is hollow, and a plurality of groups of strip-shaped holes are arranged at the front end of the storage box in a penetrating manner; the front end of the storage box is rotatably provided with a sealing cover, and a cooling mechanism communicated with an external refrigerant is arranged in the storage box and used for keeping the temperature in the storage box to be always kept at a preset temperature; the glass placing plates are arranged in the storage box in a sliding mode, the glass placing plates are arranged in the storage box in a plurality of groups, the glass placing plates are respectively fed into and discharged from the storage box through the plurality of groups of strip-shaped holes, and glass placing grooves are formed in the side walls of the glass placing plates and used for vertically placing glass slides carrying pathological change tissues.
Description
Technical Field
The invention relates to the technical field of gene detection, in particular to a kit for detecting cancer genes and a using method thereof.
Background
Clinicians find that human tumors vary widely in tumor treatment, and even if tumors are located at the same site, the treatment effect and method vary from person to person, and the treatment method for different diseases of people and diseases is called individual treatment. Therefore, only when the individual treatments are carried out according to the different diseases and different from one patient to another in the cancer treatment process, the medicines suitable for the patients can be selected according to different types of patients.
With the continuous and deep research of gene molecule level, more and more tumor cell signal channels are discovered, and a great deal of clinical research shows that the amplification/mutation/expression state of specific genes in the channels is closely related to the effectiveness of targeted and chemotherapeutic drugs. Therefore, the amplification/mutation/expression condition of specific genes in the pathways is clinically detected, and a set of most suitable treatment scheme can be customized for each patient in a targeted manner, so that the effective rate of treatment is improved to the maximum extent, the toxic and side effects of the medicines are reduced, and the improper medication time is avoided;
in the screening process of cancer gene detection, a gene detection kit is needed, the existing gene detection kit has poor tightness, gene detection results are influenced, and meanwhile, the automation degree of the kit is low, so that a plurality of groups of tissue slices are not convenient to store.
Disclosure of Invention
In order to solve the technical problems, the invention provides the kit for detecting the cancer genes and the using method thereof, which are beneficial to reducing the communication area between the outside and the inside of the storage box, improving the sealing performance of the kit and further improving the accuracy of the cancer gene detection, respectively controlling the access of a plurality of groups of glass placing plates by arranging the driving device, being beneficial to improving the automation degree of the kit and facilitating the access control of a plurality of groups of glass slides.
The kit for detecting a cancer gene of the present invention comprises:
the storage box is hollow, and a plurality of groups of strip-shaped holes are arranged at the front end of the storage box in a penetrating manner; the front end of the storage box is rotatably provided with a sealing cover, and a cooling mechanism communicated with an external refrigerant is arranged in the storage box and used for keeping the temperature in the storage box to be always kept at a preset temperature;
the glass placing plates are slidably arranged in the storage box, the glass placing plates respectively enter and exit the storage box through the strip-shaped holes, a glass placing groove is formed in the side wall of each glass placing plate and used for vertically placing a glass slide with lesion tissues, and a clamping device is arranged in each glass placing plate and used for limiting and fixing the glass slide after the glass slide is placed;
the bottom of the storage box is fixedly provided with a driving device, and the driving device is used for independently driving a plurality of groups of glass plates to enter and exit from the storage box through the strip-shaped holes.
Furthermore, the top end inside the storage box is provided with a sliding groove corresponding to each group of glass placing plates, a sliding block is fixedly installed at the rear part of one end, close to the sliding groove, of each glass placing plate, the sliding block is installed inside the sliding groove in a sliding mode, and the driving device comprises a rotating shaft rotatably installed inside the storage box and a plurality of groups of gears rotatably installed on the rotating shaft;
the bottom of each group of glass placing plates is provided with a rack, a plurality of groups of gears are respectively meshed with the racks at the bottom ends of the plurality of groups of glass placing plates, a synchronizing device is arranged on the rotating shaft corresponding to each group of gears and is used for controlling the corresponding gears to synchronously rotate or relatively rotate with the rotating shaft;
and a driving motor is fixedly installed on the inner wall of the storage box and is used for driving the rotating shaft to rotate along the axis of the rotating shaft.
Furthermore, a tapered groove is formed in one end of the gear, the synchronizing device comprises a first sleeve ring which is slidably mounted on the rotating shaft and a second sleeve ring which is rotatably mounted on the rotating shaft, a tapered ring with the same taper as the tapered groove is fixedly arranged at one end, close to the tapered groove, of the first sleeve ring, and the first sleeve ring is rotatably connected with the second sleeve ring;
connecting arms are fixedly arranged on the circumferential outer wall of the second lantern ring, a first adjusting groove is formed in the front end of the storage box corresponding to each group of connecting arms, a first shifting block is fixedly arranged at the other end of each connecting arm, and the first shifting block is slidably arranged in the first adjusting groove;
be provided with the guide way on the circumference outer wall of pivot, the guide way is parallel with the pivot axis, every group all fixed direction arch that is provided with on the inner wall of toper ring, a plurality of groups the equal slidable mounting of direction arch is inside the guide way for make toper ring and pivot keep synchronous rotation.
Furthermore, a plurality of groups of first convex edges are arranged on the conical surface of the conical ring, and a plurality of groups of second convex edges are arranged on the conical surface of the conical groove.
Furthermore, each group of glass placing plates is provided with a U-shaped groove, the U-shaped groove is positioned outside the glass placing groove and is used for installing a clamping device, and the clamping device comprises a plurality of groups of ejector rods which are inserted between the glass placing groove and the U-shaped groove and a U-shaped frame which is slidably installed inside the U-shaped groove;
each group of glass placing plates are provided with a plurality of groups of embedded grooves for accommodating the stop blocks, and each group of glass placing plates hermetically cover the U-shaped groove through a U-shaped cover plate;
correspond every group atress calotte on two sets of inner walls that U type frame is relative and all be provided with the boss, it is excessive through the inclined plane between boss and the U type frame inner wall, the both ends of U type frame all are through two sets of tip elastic connection in second spring and U type groove, the top front portion of putting the glass board is provided with the second adjustment tank, second adjustment tank intercommunication U type groove and outside, the fixed second shifting block that is provided with on the U type frame, second shifting block slidable mounting is inside the second adjustment tank.
Furthermore, the front end of the storage box is provided with four groups of sealing strips in a rectangular shape, the four groups of sealing strips are positioned outside the plurality of groups of strip-shaped holes around the formed rectangular shape, and four groups of pressing strips are arranged on the sealing cover corresponding to the four groups of sealing strips.
Furthermore, every group the sealing strip all has two sets of hollow rubber strips symmetric connection to constitute, and two sets of be provided with the draw-in groove that supplies the layering can extrude to get into between the hollow rubber strip.
Furthermore, a lock cylinder is arranged on the sealing cover, and a lock body is arranged at the front end of the storage box corresponding to the lock cylinder.
In addition, the method for using the kit for detecting the cancer gene comprises the following steps:
s1, soaking the cancerous tissue blocks for 24-36 h by using 10% formalin solution;
s2, sequentially using 80% alcohol, 90% alcohol and absolute alcohol to perform gradient ethanol dehydration on the cancerous tissue block obtained by soaking in the S1;
s3, carrying out soaking and transparent treatment on the dehydrated cancerous tissue blocks in the S2 by using dimethylbenzene;
s4, completely immersing the cancer tissue block which is subjected to the transparentization treatment in the S3 into molten paraffin for soaking for 45min, taking out and airing for 30min, repeating the processes of immersing into the molten paraffin, taking out and airing for 3-5 times, and completing paraffin embedding of the cancer tissue block;
s5, slicing the paraffin-embedded cancerous tissue block in the S4 to the thickness of 4 microns, and clamping the cancerous tissue block by using an ultraviolet-sterilized glass slide;
s6, placing the slide which is clamped with the cancerous tissue blocks in the step S5 in an idle glass placing plate on the kit for detecting the cancer genes, retracting the glass placing plate carrying the slide into the storage box by controlling a driving device in the storage box, communicating a cooling mechanism with an external refrigerant output end, keeping the temperature in the storage box at a certain temperature, and preventing the solidified paraffin in the slide from melting.
Compared with the prior art, the invention has the beneficial effects that: be used for supplying the glass board business turn over of putting of placing the slide through setting up the bar hole, be favorable to reducing the external intercommunication area with the storage box is inside, promote the leakproofness of kit, and then promote the accuracy that cancer gene detected, put the glass board through setting up drive arrangement and control a plurality of groups respectively and come in and go out, be favorable to promoting the degree of automation of kit, be convenient for carry out access control respectively to a plurality of groups slide.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the seal of the present invention shown open;
FIG. 3 is a schematic view of the structure of the interior of the storage box;
FIG. 4 is an enlarged schematic view of a gear and rack-like structural connection;
FIG. 5 is an enlarged view of the structure of the portion B in FIG. 4;
FIG. 6 is an enlarged schematic view of the structure of the gear;
FIG. 7 is an enlarged schematic view of the structural connection of the first collar to the second collar;
FIG. 8 is an enlarged view of the structure of portion A in FIG. 2;
FIG. 9 is a right enlarged view of the structure of the glass placing plate;
FIG. 10 is an exploded view of the structure of the glass panel;
in the drawings, the reference numbers: 1. a storage box; 2. a sealing cover; 3. a strip-shaped hole; 4. placing a glass plate; 5. placing a glass groove; 6. a chute; 7. a slider; 8. a rotating shaft; 9. a rack; 10. a gear; 11. a tapered groove; 12. a first collar; 13. a conical ring; 14. a second collar; 15. a connecting arm; 16. a first regulating groove; 17. a first shifting block; 18. a guide groove; 19. a guide projection; 20. a first rib; 21. a second rib; 22. a drive motor; 23. a U-shaped groove; 24. a top rod; 25. a force dome; 26. a stopper; 27. a first spring; 28. an embedded groove is formed; 29. a U-shaped frame; 30. a boss; 31. a second spring; 32. a second shifting block; 33. a second regulating groove; 34. a U-shaped cover plate; 35. a sealing strip; 36. a hollow rubber strip; 37. a card slot; 38. a lock cylinder; 39. and a lock body.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like are based on the directions or positional relationships shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; either directly or indirectly through intervening media, or may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art. This embodiment is written in a progressive manner.
As shown in fig. 1 to 3, the kit for detecting a cancer gene of the present invention includes:
the storage box comprises a storage box 1, wherein the storage box 1 is hollow, and a plurality of groups of strip-shaped holes 3 are arranged at the front end of the storage box 1 in a penetrating manner; the front end of the storage box 1 is rotatably provided with a sealing cover 2, and a cooling mechanism communicated with an external refrigerant is arranged in the storage box 1 and used for keeping the temperature in the storage box 1 to be always kept at a preset temperature;
the glass placing plate 4 is arranged, a plurality of groups of glass placing plates 4 are slidably arranged in the storage box 1, the plurality of groups of glass placing plates 4 respectively enter and exit the storage box 1 through a plurality of groups of strip-shaped holes 3, a glass placing groove 5 is formed in the side wall of each glass placing plate 4 and used for vertically placing a glass slide with pathological change tissues, and a clamping device is arranged in each glass placing plate 4 and used for limiting and fixing the glass slide after the glass slide is placed;
the bottom of the storage box 1 is fixedly provided with a driving device which is used for independently driving a plurality of groups of glass placing plates 4 to enter and exit the storage box 1 through strip-shaped holes 3;
in this embodiment, be used for supplying the business turn over of putting glass board 4 of placing the slide through setting up bar hole 3, be favorable to reducing the external and 1 inside intercommunication areas of storage box, promote the leakproofness of kit, and then promote the accuracy that cancer gene detected, put glass board 4 through setting up drive arrangement and control a plurality of groups respectively and come in and go out, be favorable to promoting the degree of automation of kit, be convenient for carry out the access control respectively to a plurality of groups slide.
As shown in fig. 3 to 5, preferably, the top end inside the storage box 1 is provided with a sliding groove 6 corresponding to each group of glass placing plates 4, a sliding block 7 is fixedly installed at the rear part of one end of each glass placing plate 4 close to the sliding groove 6, the sliding block 7 is slidably installed inside the sliding groove 6, and the driving device includes a rotating shaft 8 rotatably installed inside the storage box 1 and a plurality of groups of gears 10 rotatably installed on the rotating shaft 8;
the bottom of each group of glass placing plates 4 is provided with a rack 9, a plurality of groups of gears 10 are respectively meshed with the racks 9 at the bottom ends of the plurality of groups of glass placing plates 4, the rotating shaft 8 is provided with a synchronizer corresponding to each group of gears 10, and the synchronizer is used for controlling the corresponding gears 10 to synchronously rotate or relatively rotate with the rotating shaft 8;
a driving motor 22 is fixedly installed on the inner wall of the storage box 1, and the driving motor 22 is used for driving the rotating shaft 8 to rotate along the axis of the rotating shaft;
in the embodiment, the gear 10 is independently controlled through the rotating shaft 8 and a plurality of groups of synchronizing devices, and the independent control of the opposite glass plates 4 is realized under the matching action of the gear 10 and the rack 9, so that the transmission structure is simple, and the operation is stable.
As shown in fig. 5 to 8, preferably, one end of the gear 10 is provided with a tapered groove 11, the synchronizing device includes a first collar 12 slidably mounted on the rotating shaft 8 and a second collar 14 rotatably mounted on the rotating shaft 8, one end of the first collar 12 close to the tapered groove 11 is fixedly provided with a tapered ring 13 having the same taper as the tapered groove 11, and the first collar 12 is rotatably connected with the second collar 14;
the connecting arms 15 are fixedly arranged on the circumferential outer wall of the second lantern ring 14, a first adjusting groove 16 is formed in the front end of the storage box 1 corresponding to each group of connecting arms 15, a first shifting block 17 is fixedly arranged at the other end of each connecting arm 15, and the first shifting block 17 is slidably arranged in the first adjusting groove 16;
a guide groove 18 is formed in the circumferential outer wall of the rotating shaft 8, the guide groove 18 is parallel to the axis of the rotating shaft 8, guide protrusions 19 are fixedly arranged on the inner wall of each group of conical rings 13, and a plurality of groups of guide protrusions 19 are slidably mounted in the guide groove 18 and used for enabling the conical rings 13 and the rotating shaft 8 to synchronously rotate;
in this embodiment, the first dial 17 is manually controlled to slide in the first adjustment groove 16, so that the second collar 14 drives the first collar 12 and the tapered ring 13 to move along the rotation shaft 8, the tapered ring 13 and the rotation shaft 8 are synchronously rotated by providing the guide groove 18 and the guide protrusion 19, the tapered ring 13 is inserted into the tapered groove 11 by moving to the tapered ring 13, the tapered groove 11 is attached to the tapered surface of the tapered ring 13, the tapered ring 13 and the gear 10 are synchronously rotated by friction, and the glass plate 4 is controlled to move in and out under the connecting action of the gear 10 and the rack 9.
As shown in fig. 6 to 7, preferably, the tapered surface of the tapered ring 13 is provided with a plurality of sets of first ribs 20, and the tapered surface of the tapered groove 11 is provided with a plurality of sets of second ribs 21;
in the embodiment, through the arrangement, the friction force between the tapered groove 11 and the tapered ring 13 is improved, the relative slip between the tapered groove 11 and the tapered ring 13 is reduced, and the stability of transmission is improved.
Preferably, as shown in fig. 9 to 10, each group of glass placing plates 4 is provided with a U-shaped groove 23, the U-shaped groove 23 is located outside the glass placing groove 5, the U-shaped groove 23 is used for installing a clamping device, and the clamping device includes a plurality of groups of push rods 24 inserted between the glass placing groove 5 and the U-shaped groove 23 and a U-shaped frame 29 slidably installed inside the U-shaped groove 23;
one end of each group of the ejector rods 24, which is positioned in the U-shaped groove 23, is provided with a stressed dome 25, one end of each group of the ejector rods 24, which is positioned in the glass placing groove 5, is fixedly provided with a stop dog 26, each group of the ejector rods 24 is sleeved with a first spring 27, the first spring 27 is positioned between the stressed dome 25 and the inner wall of the U-shaped groove 23, the inner wall of the glass placing groove 5 is provided with a plurality of groups of embedded grooves 28 for accommodating the stop dogs 26, and each group of the glass placing plates 4 hermetically cover the U-shaped groove 23 through a U-shaped cover plate 34;
in this embodiment, slide in second adjustment tank 33 inside through control second shifting block 32, make U type frame 29 back-and-forth movement, in the removal process, through the extrusion of boss 30 to atress dome 25, thereby control dog 26 contracts to embedded groove 28 inside, be convenient for this moment to putting the inside slide of placing of glass groove 5, through setting up second spring 31, make a plurality of groups boss 30 on the U type frame 29 separate with a plurality of groups atress dome 25 all the time under the non-artificial effect, and under the spring action of first spring 27, make a plurality of groups dog 26 stretch into to putting inside the glass groove 5, keep spacing to the slide all the time.
Preferably, as shown in fig. 2, four groups of sealing strips 35 are arranged at the front end of the storage box 1 in a rectangular shape, the rectangle formed by the four groups of sealing strips 35 is located outside the plurality of groups of strip-shaped holes 3, and four groups of pressing strips are arranged on the sealing cover 2 corresponding to the four groups of sealing strips 35;
in the present embodiment, the sealing property of the connection between the sealing cover 2 and the storage case 1 is improved by the cooperation of the bead and the sealing strip 35.
Preferably, as shown in fig. 8, each group of the sealing strips 35 is formed by symmetrically connecting two groups of hollow rubber strips 36, and a clamping groove 37 for allowing a pressing strip to be extruded into is arranged between the two groups of the hollow rubber strips 36.
As shown in fig. 1 to 2, preferably, the sealing cover 2 is provided with a lock cylinder 38, and the front end of the storage box 1 is provided with a lock 39 corresponding to the lock cylinder 38.
The kit for detecting a cancer gene and the use method thereof of the present invention are all generally mechanically installed, connected or installed, and can be implemented as long as the advantageous effects thereof are achieved.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (7)
1. A kit for detecting a cancer gene, comprising:
the storage box (1) is hollow, and a plurality of groups of strip-shaped holes (3) are arranged at the front end of the storage box (1) in a penetrating manner; the front end of the storage box (1) is rotatably provided with a sealing cover (2), and a cooling mechanism communicated with an external refrigerant is arranged in the storage box (1) and used for keeping the temperature in the storage box (1) to be always kept at a preset temperature;
the glass placing plates (4) are arranged in the storage box (1) in a sliding mode, the glass placing plates (4) are respectively fed into and taken out of the storage box (1) through the strip-shaped holes (3), a glass placing groove (5) is formed in the side wall of each glass placing plate (4) and used for vertically placing a glass slide with a pathological change tissue, and a clamping device is arranged in each glass placing plate (4) and used for limiting and fixing the glass slide after the glass slide is placed;
the bottom of the storage box (1) is fixedly provided with a driving device, and the driving device is used for independently driving a plurality of groups of glass placing plates (4) to enter and exit the storage box (1) through the strip-shaped holes (3);
the top end inside the storage box (1) is provided with a sliding groove (6) corresponding to each group of glass placing plates (4), a sliding block (7) is fixedly installed at the rear part of one end, close to the sliding groove (6), of each glass placing plate (4), the sliding block (7) is installed inside the sliding groove (6) in a sliding mode, and the driving device comprises a rotating shaft (8) rotatably installed inside the storage box (1) and a plurality of groups of gears (10) rotatably installed on the rotating shaft (8);
the bottom of each group of glass placing plates (4) is provided with a rack (9), a plurality of groups of gears (10) are respectively meshed with the racks (9) at the bottom ends of the groups of glass placing plates (4), a synchronizing device is arranged on the rotating shaft (8) corresponding to each group of gears (10), and the synchronizing device is used for controlling the corresponding gears (10) to synchronously rotate or relatively rotate with the rotating shaft (8);
a driving motor (22) is fixedly installed on the inner wall of the storage box (1), and the driving motor (22) is used for driving the rotating shaft (8) to rotate along the axis of the rotating shaft;
one end of the gear (10) is provided with a tapered groove (11), the synchronizing device comprises a first lantern ring (12) which is slidably mounted on the rotating shaft (8) and a second lantern ring (14) which is rotatably mounted on the rotating shaft (8), one end, close to the tapered groove (11), of the first lantern ring (12) is fixedly provided with a tapered ring (13) with the same taper as the tapered groove (11), and the first lantern ring (12) is rotatably connected with the second lantern ring (14);
connecting arms (15) are fixedly arranged on the circumferential outer wall of the second lantern ring (14), a first adjusting groove (16) is formed in the front end of the storage box (1) corresponding to each group of connecting arms (15), a first shifting block (17) is fixedly arranged at the other end of each connecting arm (15), and the first shifting block (17) is slidably mounted in the first adjusting groove (16);
be provided with guide way (18) on the circumference outer wall of pivot (8), guide way (18) are parallel with pivot (8) axis, every group all fixedly on the inner wall of toper ring (13) be provided with guide protrusion (19), a plurality of groups the equal slidable mounting of guide protrusion (19) is inside guide way (18) for make toper ring (13) and pivot (8) keep synchronous rotation.
2. The kit for detecting a cancer gene according to claim 1, wherein the tapered surface of the tapered ring (13) is provided with a plurality of sets of first ribs (20), and the tapered surface of the tapered groove (11) is provided with a plurality of sets of second ribs (21).
3. The kit for cancer gene detection according to claim 1, wherein each set of the glass placing plate (4) is provided with a U-shaped groove (23), the U-shaped groove (23) is located outside the glass placing groove (5), the U-shaped groove (23) is used for installing a clamping device, and the clamping device comprises a plurality of sets of push rods (24) inserted between the glass placing groove (5) and the U-shaped groove (23) and a U-shaped frame (29) slidably installed inside the U-shaped groove (23);
one end of each group of the ejector rods (24) positioned in the U-shaped groove (23) is provided with a stressed dome (25), one end of each group of the ejector rods (24) positioned in the glass placing groove (5) is fixedly provided with a stop block (26), each group of the ejector rods (24) is sleeved with a first spring (27), the first springs (27) are positioned between the stressed domes (25) and the inner wall of the U-shaped groove (23), the inner wall of the glass placing groove (5) is provided with a plurality of groups of embedded grooves (28) for accommodating the stop blocks (26), and each group of the glass placing plates (4) hermetically cover the U-shaped groove (23) through a U-shaped cover plate (34);
correspond every group atress calotte (25) and all be provided with boss (30) on two sets of inner walls that U type frame (29) is relative, it is excessive through the inclined plane between boss (30) and U type frame (29) inner wall, the both ends of U type frame (29) all are through second spring (31) and two sets of tip elastic connection in U type groove (23), the top front portion of putting glass board (4) is provided with second adjustment tank (33), second adjustment tank (33) intercommunication U type groove (23) and outside, fixed second shifting block (32) that are provided with on U type frame (29), second shifting block (32) slidable mounting is inside second adjustment tank (33).
4. The kit for detecting cancer genes according to claim 1, wherein four sets of sealing strips (35) are arranged at the front end of the storage box (1) in a rectangular shape, the rectangle surrounded by the four sets of sealing strips (35) is positioned outside the plurality of sets of strip holes (3), and four sets of pressing strips are arranged on the sealing cover (2) corresponding to the four sets of sealing strips (35).
5. The cancer gene detection kit according to claim 4, wherein each of the sealing strips (35) is composed of two groups of hollow rubber strips (36) symmetrically connected to each other, and a slot (37) for allowing a pressing strip to be extruded into is provided between the two groups of hollow rubber strips (36).
6. The kit for cancer gene detection according to claim 1, wherein the sealing cap (2) is provided with a lock cylinder (38), and a lock (39) is provided at the front end of the storage case (1) corresponding to the lock cylinder (38).
7. The method of using the kit for detecting cancer genes according to any one of claims 1 to 6, comprising the steps of:
s1, soaking the cancerous tissue blocks for 24-36 h by using 10% formalin solution;
s2, sequentially using 80% alcohol, 90% alcohol and absolute alcohol to perform gradient ethanol dehydration on the cancerous tissue block obtained by soaking in the S1;
s3, carrying out soaking and transparent treatment on the dehydrated cancerous tissue blocks in the S2 by using dimethylbenzene;
s4, completely immersing the cancer tissue block subjected to the transparentization treatment in the S3 into molten paraffin for 45min, taking out and airing for 30min, and repeating the processes of immersing into the molten paraffin, taking out and airing for 3-5 times to complete paraffin embedding of the cancer tissue block;
s5, slicing the paraffin-embedded cancerous tissue block in the S4 to the thickness of 4 microns, and clamping the cancerous tissue block by using an ultraviolet-sterilized glass slide;
s6, placing the slide which is clamped with the cancerous tissue blocks in the S5 in an empty idle glass plate (4) on the kit for detecting the cancer genes, retracting the glass plate (4) carrying the slide into the storage box (1) by controlling a driving device in the storage box (1), and communicating a cooling mechanism with an external refrigerant output end to keep the interior of the storage box (1) at a certain temperature so as to prevent the solidified paraffin in the slide from melting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111176033.8A CN113916638B (en) | 2021-10-09 | 2021-10-09 | Kit for cancer gene detection and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111176033.8A CN113916638B (en) | 2021-10-09 | 2021-10-09 | Kit for cancer gene detection and use method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113916638A CN113916638A (en) | 2022-01-11 |
| CN113916638B true CN113916638B (en) | 2022-09-06 |
Family
ID=79238657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111176033.8A Active CN113916638B (en) | 2021-10-09 | 2021-10-09 | Kit for cancer gene detection and use method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113916638B (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203627586U (en) * | 2013-10-28 | 2014-06-04 | 长城汽车股份有限公司 | Synchronizer |
| CN107063787A (en) * | 2016-12-19 | 2017-08-18 | 浙江世纪康大医疗科技股份有限公司 | A kind of automatic pullable cover plate system |
| CN206954733U (en) * | 2017-06-16 | 2018-02-02 | 谭启涛 | Examine with sample slide record storage machine |
| CN108516227A (en) * | 2018-06-30 | 2018-09-11 | 柳州市妇幼保健院 | Automatic slide pops up machine |
| CN109443882A (en) * | 2018-12-27 | 2019-03-08 | 郑州金域临床检验中心有限公司 | A kind of chromosome sectioning device |
| CN208665838U (en) * | 2018-08-31 | 2019-03-29 | 宜昌微诺科技有限公司 | A kind of medical smear placement transport case |
| CN208699355U (en) * | 2018-04-28 | 2019-04-05 | 江西津昌干细胞基因工程有限公司 | A kind of candidate stem cell storage contrast means |
| CN209939287U (en) * | 2018-03-06 | 2020-01-14 | 宋莎莎 | Pathology department's section access arrangement |
| CN210923445U (en) * | 2019-09-29 | 2020-07-03 | 湖南国科智瞳科技有限公司 | Object carrying platform applied to pathological section scanner |
| CN212049414U (en) * | 2019-12-18 | 2020-12-01 | 深圳北航新兴产业技术研究院 | Continuous automatic sample feeding and discharging device for glass sheet box |
| CN112096752A (en) * | 2020-10-10 | 2020-12-18 | 綦江永跃齿轮有限公司 | Anti-clamping stagnation synchronizer |
| CN112407550A (en) * | 2020-10-19 | 2021-02-26 | 佛山市乙太医疗用品有限公司 | Sample storage device for medical research |
| CN213800786U (en) * | 2020-10-21 | 2021-07-27 | 孙诗雨 | Reagent storage box suitable for medical chemical examination branch of academic or vocational study is used |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112619732A (en) * | 2020-12-18 | 2021-04-09 | 杨小华 | Multifunctional medical kit |
-
2021
- 2021-10-09 CN CN202111176033.8A patent/CN113916638B/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203627586U (en) * | 2013-10-28 | 2014-06-04 | 长城汽车股份有限公司 | Synchronizer |
| CN107063787A (en) * | 2016-12-19 | 2017-08-18 | 浙江世纪康大医疗科技股份有限公司 | A kind of automatic pullable cover plate system |
| CN206954733U (en) * | 2017-06-16 | 2018-02-02 | 谭启涛 | Examine with sample slide record storage machine |
| CN209939287U (en) * | 2018-03-06 | 2020-01-14 | 宋莎莎 | Pathology department's section access arrangement |
| CN208699355U (en) * | 2018-04-28 | 2019-04-05 | 江西津昌干细胞基因工程有限公司 | A kind of candidate stem cell storage contrast means |
| CN108516227A (en) * | 2018-06-30 | 2018-09-11 | 柳州市妇幼保健院 | Automatic slide pops up machine |
| CN208665838U (en) * | 2018-08-31 | 2019-03-29 | 宜昌微诺科技有限公司 | A kind of medical smear placement transport case |
| CN109443882A (en) * | 2018-12-27 | 2019-03-08 | 郑州金域临床检验中心有限公司 | A kind of chromosome sectioning device |
| CN210923445U (en) * | 2019-09-29 | 2020-07-03 | 湖南国科智瞳科技有限公司 | Object carrying platform applied to pathological section scanner |
| CN212049414U (en) * | 2019-12-18 | 2020-12-01 | 深圳北航新兴产业技术研究院 | Continuous automatic sample feeding and discharging device for glass sheet box |
| CN112096752A (en) * | 2020-10-10 | 2020-12-18 | 綦江永跃齿轮有限公司 | Anti-clamping stagnation synchronizer |
| CN112407550A (en) * | 2020-10-19 | 2021-02-26 | 佛山市乙太医疗用品有限公司 | Sample storage device for medical research |
| CN213800786U (en) * | 2020-10-21 | 2021-07-27 | 孙诗雨 | Reagent storage box suitable for medical chemical examination branch of academic or vocational study is used |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113916638A (en) | 2022-01-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2294321T3 (en) | MANUAL CONSTRUCTION ASSEMBLY OF TISSULAR MICROMATRICES. | |
| US20160242748A1 (en) | Device and method for handling biological tissues | |
| ES2331888T3 (en) | CYLINDER TAKING DEVICE FOR FABRICS MATRICES. | |
| CN113916638B (en) | Kit for cancer gene detection and use method thereof | |
| CN109700483B (en) | Biopsy sampling device that tumour administrative or technical offices used | |
| CN212730266U (en) | Convenient medicine taking device for nursing department | |
| CN215227909U (en) | Specimen box for mammary gland surgery | |
| CN211921521U (en) | Lung cancer circulating tumor cell detection device | |
| CN211022799U (en) | Kidney is biopsy piercing depth for internal medicine | |
| CN215015200U (en) | A plasma storage cabinet for physiology | |
| CN219889862U (en) | Medical refrigerator convenient to remove | |
| CN217907985U (en) | Movable tray rack | |
| CN110123284A (en) | A kind of flap capillary reflux state monitoring device | |
| CN211246617U (en) | Special semen replacement prevention delivery inspection device for reproductive center | |
| CN211430785U (en) | Stem cell storage device convenient to use | |
| CN210419910U (en) | A extraction element for biological gene sample | |
| CN215197066U (en) | PCR molecular diagnosis sample strorage device | |
| CN218679900U (en) | Culture dish with separation structure | |
| CN213951223U (en) | Cell culture device convenient to tumour experiment is observed | |
| CN213951197U (en) | Novel rapid detection kit for HER2 gene amplification | |
| CN215938302U (en) | Clinical medication guiding tool for diabetes | |
| CN215190239U (en) | Multi-functional intelligent filing cabinet based on fingerprint identification | |
| CN220674976U (en) | Organoid freezes deposits cooling box | |
| CN218047959U (en) | Blood sample collection box for hematology nursing | |
| CN212476692U (en) | Chimeric antigen receptor T cell preparation device for treating AML |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |