CN113908198A - A composition for preventing and treating prostate diseases, and its preparation method - Google Patents
A composition for preventing and treating prostate diseases, and its preparation method Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
- A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/30—Zinc; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/2027—Organic macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
- A61K9/2054—Cellulose; Cellulose derivatives, e.g. hydroxypropyl methylcellulose
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/08—Drugs for disorders of the urinary system of the prostate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
Abstract
The invention discloses a composition for preventing and treating prostate diseases and a preparation method thereof. The composition for preventing and treating prostate diseases takes freeze-dried rape bee pollen, raspberry freeze-dried powder, zinc-rich yeast, arginine and aescin powder as main raw materials. By combining modern scientific technology and processing the rape bee pollen in a pulse electric field wall breaking mode, a layer of bee pollen cell has certain resistance to acid, alkali, high temperature and high pressure processing, the outer wall is broken, the intracellular substances are quickly released, the nutrient components of the raw materials are better reserved, and the absorption by a human body is facilitated. The organic synthetic zinc is adopted as the raw material of the zinc-rich yeast, so that the irritation to intestines and stomach is small, and the zinc-rich yeast can be better absorbed by human bodies. Meanwhile, the aescin powder is added to improve the symptoms of frequent micturition, urgent micturition and urge incontinence. Can effectively prevent and treat prostate diseases.
Description
Technical Field
The invention relates to the field of pharmacy, in particular to a composition for preventing and treating prostate diseases and a preparation method thereof.
Background
The prostate diseases such as prostatitis and prostatic hyperplasia are common diseases for middle-aged and old men. Nonbacterial Prostatitis (CNP) is one of the most common urological diseases. Inflammation can cause a series of immune reactions, prostate swelling, urethra compression deformation and stenosis, which causes dysuria, frequent micturition, pain in urination, and lower abdominal internal radiation pain in the whole urination process. And because the permeability of the prostatic membrane is poor, common medicines can hardly reach the focus, so that special medicines for treating prostatic diseases are few.
Wherein, the rape bee pollen can resist bacteria inflammation, resist oxidation and reduce the permeability of capillary vessels, improve the hyperplasia of prostate tissue by adjusting the release of PGE2, activate the activity of lysosome in hyperplastic cells, effectively improve BPH symptoms and complications and inhibit the hyperplasia of prostate cells in vitro. Moreover, the rape bee pollen obtained by the treatment of the pulsed electric field wall breaking way can better retain the nutrient components of the raw materials and is beneficial to the absorption of human body.
Meanwhile, the organic synthetic zinc is adopted as the raw material of the zinc-rich yeast, so that the irritation to intestines and stomach is small, and the zinc-rich yeast can be better absorbed by human bodies. The added aescin powder has the functions of eliminating swelling, relieving pain, diminishing inflammation, resisting exudation, protecting blood vessel, improving blood circulation, increasing vein tension, eliminating active oxygen and improving frequent micturition, urgent micturition and urge incontinence.
Therefore, it is of great significance to prepare a composition for preventing and treating prostate diseases by solving the above problems.
Disclosure of Invention
The present invention is directed to a composition for preventing and treating prostate diseases and a method for preparing the same, which solves the problems of the background art described above.
In order to solve the technical problems, the invention provides the following technical scheme:
a method for preparing a composition for preventing and treating prostate diseases, which is characterized by comprising the following steps: the method comprises the following steps:
step 1: removing impurities from rape bee pollen, grinding, preparing rape bee pollen suspension, treating with a pulse electric field, freezing at-18 to-22 ℃ for 24 to 28 hours, adding 80 to 86 ℃ hot water, stirring, cooling to 42 to 46 ℃, continuously stirring at the constant temperature of 44 to 46 ℃ for 6 to 8 hours, freezing, and drying to obtain wall-broken rape bee pollen;
step 2: putting the wall-broken rape bee pollen into a freeze dryer, pre-freezing, sublimating for 2-4 h at the temperature of-40 to-45 ℃, resolving for 2-4 h, vacuum drying for 2-4 h at the vacuum degree of 50-60 pa and the temperature of 46-50 ℃ to obtain freeze-dried rape bee pollen;
and step 3: putting the raspberry into a freeze dryer for pre-freezing, sublimating for 2-4 hours at the temperature of-35 to-40 ℃, resolving for 2-4 hours, and vacuum drying for 2-4 hours at the vacuum degree of 50-60 pa and the temperature of 46-50 ℃ to obtain raspberry freeze-dried powder;
and 4, step 4: mixing lyophilized bee pollen of Brassica campestris, arginine and polyvinylpolypyrrolidone, drying, pulverizing, and granulating to obtain a premix;
and 5: uniformly mixing the premix, raspberry freeze-dried powder, aescin powder and zinc-rich yeast, adding microcrystalline cellulose, hydroxypropyl methylcellulose and magnesium stearate, uniformly mixing, drying, crushing and sieving by a 30-mesh sieve; granulating, drying by air at 60-66 ℃ for 2-3 h, cooling to room temperature, controlling the hardness of the tablets, and tabletting to obtain the product.
Preferably, the composition comprises the following components: 150-160 parts of freeze-dried rape bee pollen, 110-112 parts of raspberry, 90-96 parts of zinc-rich yeast, 130-136 parts of arginine, 10-20 parts of aescin powder, 20-30 parts of crospovidone, 20-30 parts of microcrystalline cellulose, 10-20 parts of hydroxypropyl methylcellulose and 10-20 parts of magnesium stearate.
Preferably, in step 1, uniformly grinding the rape bee pollen, adding 80% ethanol solution, and stirring for 3-5h to obtain rape bee pollen suspension.
Preferably, in the step 1, warm water at 44-46 ℃ is added once every 1-2 hours when the mixture is continuously stirred at the constant temperature of 44-46 ℃.
Preferably, in the step 1, the pulsed electric field treatment is to pump the rape bee pollen suspension into a pulsed electric field treatment chamber by a pump, and after air in the treatment chamber and a pipeline is exhausted, the flow rate of the bee pollen liquid is controlled to be 80-90 mL/min; the pulse electric field treatment conditions are as follows: the pulse frequency is 1kHz, the pulse width is 40 mus, the waveform is a bipolar pulse square wave, the electric field intensity is 26.67kV/cm, and the number of processing pulses is respectively 0, 120, 240, 360, 400 and 600.
Optimally, in the step 2, the pre-freezing temperature of the rape bee pollen is-36 to-40 ℃.
Preferably, in the step 3, the pre-freezing temperature of the raspberry is-30 to-36 ℃.
Preferably, in step 4, the zinc in the zinc-rich yeast is organically synthesized zinc, and comprises any one of zinc gluconate, zinc oxalate, zinc citrate, zinc lactate and zinc glycyrrhetate.
In the technical scheme:
(1) the pollen is rape bee pollen containing oximinoic acid as main component for resisting prostatic hyperplasia. Compared with other pollen, the rape bee pollen has higher flavonoid content, can resist bacteria, diminish inflammation, resist oxidation and reduce the permeability of capillary vessels, improves the hyperplasia of prostate tissue by adjusting the release of PGE2, can activate the activity of lysosomes in hyperplastic cells, can effectively improve BPH symptoms and complications and inhibit the hyperplasia of prostate cells in vitro. Polyamines in pollen are mainly involved in biosynthesis and stability regulation of cellular DNA, RNA and protein, and play an important role in regulation of cell growth, proliferation and differentiation. Especially, the feruloylputrescine and the cadaverine can relax the tension of smooth muscles of the urinary system and are beneficial to urination.
(2) The bee pollen is extracted by breaking cell wall with pulse electric field, and compared with other cell wall breaking methods, the cell membrane can be broken by the pulse electric field wall breaking method, and intracellular substances can be released rapidly. Compared with the prior art, the method has the advantages of short treatment time, low energy consumption, obvious sterilization effect, small temperature rise, obvious wall breaking effect on the rape bee pollen and the like, and the process of breaking the wall by the pulse electric field is carried out at room temperature, the treatment condition is mild, and the nutrient components in the rape bee pollen can be retained to the maximum extent.
(3) The preparation of the rape bee pollen and the raspberry adopts freeze-drying preparation, the freeze-drying preparation is different from the traditional drying technology, the vacuum freeze-drying is utilized, the sublimation is carried out in a sublimation mode, the solid state is subjected to water sublimation, the influence on the rape bee pollen and the raspberry is small, the damage to a biological structure is avoided, the cell activity is kept, and the quality of a finished medicine product is improved; the method is carried out at a lower temperature, and does not affect the protein. The medicine is treated in a vacuum environment, so that the interference of external factors can be reduced, impurities are prevented from being generated, and the sterilization effect is achieved.
(4) Zinc-rich yeast is added. Zinc deficiency can cause sexual maturity obstacle, zinc can improve sperm survival rate and enhance reproductive capacity, and zinc-rich yeast is more beneficial to absorption and utilization of human body than zinc. Wherein, the zinc in the zinc-rich yeast is organic synthetic zinc, compared with inorganic zinc, the zinc-rich yeast has obviously reduced stimulation effect on gastrointestinal tracts, and the oral absorption utilization rate is improved compared with non-polar zinc.
(5) The aescin powder is added, and is an extract of seeds of Aesculus hippocastanum, and has effects of relieving edema, relieving swelling and pain, resisting inflammation, resisting exudation, protecting blood vessel, improving blood circulation, increasing venous tension, removing active oxygen, and promoting early improvement of pollakisuria, urgent micturition, urge incontinence, etc.
(6) The freeze-dried rape bee pollen, the arginine and the crospovidone are uniformly mixed, so that the freeze-dried rape bee pollen and the arginine are quickly cracked into fine particles in gastrointestinal fluid, and the freeze-dried rape bee pollen and the arginine can be quickly dissolved and absorbed by a human body to play a role. Furthermore, the interaction between the freeze-dried rape bee pollen and arginine can promote the secretion of insulin and ensure that the blood sugar is in a normal range.
(7) After the microcrystalline cellulose is added, the mixture is dried, so that the premix, the raspberry freeze-dried powder, the aescin powder and the zinc-rich yeast mixture can be effectively prevented from being evenly divided into particles, and the active ingredients are not crystallized and kept stable at high temperature.
(8) The hydroxypropyl methylcellulose is added to prepare the soft material, so that on one hand, the particle shape and the particle division of the medicine can be controlled, and simultaneously, the slow-release control effect is achieved, the medicine can be slowly released at a constant speed, the blood concentration is promoted to be stable, and the medicine is basically absorbed.
Compared with the prior art, the invention has the following beneficial effects: (1) the rape bee pollen is extracted by adopting pulse electric field wall breaking, compared with other wall breaking methods, the pulse electric field wall breaking method can break cell membranes and quickly release substances in cells. Compared with the prior art, the method has the advantages of short treatment time, low energy consumption, obvious sterilization effect, small temperature rise, obvious wall breaking effect on the rape bee pollen and the like, and the process of breaking the wall by the pulse electric field is carried out at room temperature, the treatment condition is mild, and the nutrient components in the rape bee pollen can be retained to the maximum extent. (2) The organic synthetic zinc is adopted, the stimulation effect on the gastrointestinal tract is obviously reduced, and the oral absorption utilization rate is improved compared with that of electrodeless zinc. (3) The obtained aescin powder has effects in relieving edema, relieving swelling and pain, relieving inflammation, resisting exudation, protecting blood vessel, improving blood circulation, increasing venous tension, and eliminating active oxygen, and can be used for improving frequent micturition, urgent micturition, and urge incontinence.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
step 1: removing impurities from 155 parts of rape bee pollen, grinding, adding 120ml of 80% ethanol solution, and stirring for 4h to obtain rape bee pollen suspension. Pumping the rape bee pollen suspension into a pulsed electric field treatment chamber by a pump, and controlling the flow rate of the rape bee pollen suspension at 85mL/min after air in the treatment chamber and a pipeline is exhausted. The pulse electric field treatment conditions are as follows: the pulse frequency is 1kHz, the pulse width is 40 mus, the waveform is a bipolar pulse square wave, the electric field intensity is 26.67kV/cm, and the number of processing pulses is respectively 0, 120, 240, 360, 400 and 600. Freezing at-20 deg.C for 26h, adding 83 deg.C hot water, stirring, cooling to 44 deg.C, continuously stirring at constant temperature of 45 deg.C for 7h, adding 45 deg.C warm water once every 1.5h, freezing, and drying to obtain wall-broken caulis et folium Brassicae campestris bee pollen.
Step 2: putting the wall-broken rape bee pollen into a freeze dryer, pre-freezing, sublimating for 3h at-43 deg.C, resolving for 3h, vacuum drying for 3h at vacuum degree of 55pa and temperature of 48 deg.C to obtain freeze-dried rape bee pollen.
And step 3: putting 111 parts of raspberry into a freeze dryer, pre-freezing, sublimating for 3 hours at-38 ℃, resolving for 3 hours, and vacuum drying for 3 hours at 55pa of vacuum degree and 48 ℃ to obtain raspberry freeze-dried powder.
And 4, step 4: the freeze-dried rape bee pollen, 133 parts of arginine and 25 parts of crospovidone are uniformly mixed, dried, crushed and granulated to obtain the premix.
And 5: uniformly mixing the premix, raspberry freeze-dried powder, 15 parts of aescin powder and 93 parts of zinc-rich yeast, adding 25 parts of microcrystalline cellulose, 15 parts of hydroxypropyl methylcellulose and 15 parts of magnesium stearate, uniformly mixing, drying, crushing and sieving by a 30-mesh sieve; granulating, drying by air at 60-66 ℃ for 2-3 h, cooling to room temperature, controlling the hardness of the tablets, and tabletting to obtain the product.
Example 2:
step 1: removing impurities from 150 parts of rape bee pollen, grinding, adding 120ml of 80% ethanol solution, and stirring for 3h to obtain rape bee pollen suspension. Pumping the rape bee pollen suspension into a pulsed electric field treatment chamber by a pump, and controlling the flow rate of the rape bee pollen suspension at 80mL/min after air in the treatment chamber and a pipeline is exhausted. The pulse electric field treatment conditions are as follows: the pulse frequency is 1kHz, the pulse width is 40 mus, the waveform is a bipolar pulse square wave, the electric field intensity is 26.67kV/cm, and the number of processing pulses is respectively 0, 120, 240, 360, 400 and 600. Freezing at-18 deg.C for 24 hr, adding 80 deg.C hot water, stirring, cooling to 42 deg.C, continuously stirring at constant temperature of 44 deg.C for 6 hr, adding 44 deg.C warm water every 1 hr, freezing, and drying to obtain wall-broken caulis et folium Brassicae campestris bee pollen.
Step 2: putting the wall-broken rape bee pollen into a freeze dryer, pre-freezing, sublimating for 2h at-40 deg.C, resolving for 2h, vacuum drying for 2h at vacuum degree of 50pa and temperature of 46 deg.C to obtain freeze-dried rape bee pollen.
And step 3: putting 110 parts of raspberry into a freeze dryer, pre-freezing, sublimating for 2h at-35 ℃, resolving for 2h, and vacuum drying for 2h at the vacuum degree of 50pa and the temperature of 46 ℃ to obtain raspberry freeze-dried powder.
And 4, step 4: the freeze-dried rape bee pollen, 130 parts of arginine and 20 parts of crospovidone are uniformly mixed, dried, crushed and granulated to obtain the premix.
And 5: uniformly mixing the premix, raspberry freeze-dried powder, 10 parts of aescin powder and 90 parts of zinc-rich yeast, adding 20 parts of microcrystalline cellulose, 10 parts of hydroxypropyl methylcellulose and 10 parts of magnesium stearate, uniformly mixing, drying, crushing and sieving by a 30-mesh sieve; granulating, drying by air at 60-66 ℃ for 2-3 h, cooling to room temperature, controlling the hardness of the tablets, and tabletting to obtain the product.
Example 3:
step 1: removing impurities from 160 parts of rape bee pollen, grinding, adding 120ml of 80% ethanol solution, and stirring for 5h to obtain rape bee pollen suspension. Pumping the rape bee pollen suspension into a pulsed electric field treatment chamber by a pump, and controlling the flow rate of the rape bee pollen suspension at 90mL/min after air in the treatment chamber and a pipeline is exhausted. The pulse electric field treatment conditions are as follows: the pulse frequency is 1kHz, the pulse width is 40 mus, the waveform is a bipolar pulse square wave, the electric field intensity is 26.67kV/cm, and the number of processing pulses is respectively 0, 120, 240, 360, 400 and 600. Freezing at-22 deg.C for 28h, adding 86 deg.C hot water, stirring, cooling to 46 deg.C, continuously stirring at constant temperature of 46 deg.C for 8h, adding 46 deg.C warm water every 2h, freezing, and drying to obtain wall-broken caulis et folium Brassicae campestris bee pollen.
Step 2: putting the wall-broken rape bee pollen into a freeze dryer, pre-freezing, sublimating for 4h at-45 deg.C, resolving for 4h, vacuum drying for 4h at vacuum degree of 60pa and temperature of 50 deg.C to obtain freeze-dried rape bee pollen.
And step 3: putting 112 parts of raspberry into a freeze dryer, pre-freezing, sublimating for 4 hours at the temperature of-40 ℃, resolving for 4 hours, and vacuum drying for 4 hours at the vacuum degree of 60pa and the temperature of 50 ℃ to obtain raspberry freeze-dried powder.
And 4, step 4: the freeze-dried rape bee pollen, 136 parts of arginine and 30 parts of crospovidone are uniformly mixed, dried, crushed and granulated to obtain the premix.
And 5: uniformly mixing the premix, raspberry freeze-dried powder, 20 parts of aescin powder and 96 parts of zinc-rich yeast, adding 30 parts of microcrystalline cellulose, 20 parts of hydroxypropyl methylcellulose and 20 parts of magnesium stearate, uniformly mixing, drying, crushing and sieving by a 30-mesh sieve; granulating, drying by air at 60-66 ℃ for 2-3 h, cooling to room temperature, controlling the hardness of the tablets, and tabletting to obtain the product.
Example 4:
step 1: removing impurities from 155 parts of bee pollen, grinding, adding 120ml of 80% ethanol solution, and stirring for 4h to obtain a bee pollen suspension. Pumping the bee pollen suspension into the pulsed electric field treatment chamber by a pump, and controlling the flow rate of the bee pollen suspension at 85mL/min after the air in the treatment chamber and the pipeline is exhausted. The pulse electric field treatment conditions are as follows: the pulse frequency is 1kHz, the pulse width is 40 mus, the waveform is a bipolar pulse square wave, the electric field intensity is 26.67kV/cm, and the number of processing pulses is respectively 0, 120, 240, 360, 400 and 600. Freezing at-20 deg.C for 26h, adding 83 deg.C hot water, stirring, cooling to 44 deg.C, continuously stirring at constant temperature of 45 deg.C for 7h, adding 45 deg.C warm water at intervals of 1.5h, freezing, and drying to obtain wall-broken bee pollen.
Step 2: putting the wall-broken bee pollen into a freeze dryer, pre-freezing, sublimating at-43 deg.C for 3h, resolving for 3h, vacuum drying for 3h at vacuum degree of 55pa and temperature of 48 deg.C to obtain lyophilized bee pollen.
And step 3: putting 111 parts of raspberry into a freeze dryer, pre-freezing, sublimating for 3 hours at-38 ℃, resolving for 3 hours, and vacuum drying for 3 hours at 55pa of vacuum degree and 48 ℃ to obtain raspberry freeze-dried powder.
And 4, step 4: the freeze-dried bee pollen, 133 parts of arginine and 25 parts of crospovidone are uniformly mixed, dried, crushed and granulated to obtain the premix.
And 5: uniformly mixing the premix, raspberry freeze-dried powder, 15 parts of aescin powder and 93 parts of zinc-rich yeast, adding 25 parts of microcrystalline cellulose, 15 parts of hydroxypropyl methylcellulose and 15 parts of magnesium stearate, uniformly mixing, drying, crushing and sieving by a 30-mesh sieve; granulating, drying by air at 60-66 ℃ for 2-3 h, cooling to room temperature, controlling the hardness of the tablets, and tabletting to obtain the product.
Example 5:
step 1: removing impurities from 155 parts of rape bee pollen, grinding, adding 120ml of 80% ethanol solution, and stirring for 4h to obtain rape bee pollen suspension. Pumping the rape bee pollen suspension into a pulsed electric field treatment chamber by a pump, and controlling the flow rate of the rape bee pollen suspension at 85mL/min after air in the treatment chamber and a pipeline is exhausted. The pulse electric field treatment conditions are as follows: the pulse frequency is 1kHz, the pulse width is 40 mus, the waveform is a bipolar pulse square wave, the electric field intensity is 26.67kV/cm, and the number of processing pulses is respectively 0, 120, 240, 360, 400 and 600. Freezing at-20 deg.C for 26h, adding 83 deg.C hot water, stirring, cooling to 44 deg.C, continuously stirring at constant temperature of 45 deg.C for 7h, adding 45 deg.C warm water once every 1.5h, freezing, and drying to obtain wall-broken caulis et folium Brassicae campestris bee pollen.
Step 2: putting the wall-broken rape bee pollen into a freeze dryer, pre-freezing, sublimating for 3h at-43 deg.C, resolving for 3h, vacuum drying for 3h at vacuum degree of 55pa and temperature of 48 deg.C to obtain freeze-dried rape bee pollen.
And step 3: putting 111 parts of raspberry into a freeze dryer, pre-freezing, sublimating for 3 hours at-38 ℃, resolving for 3 hours, and vacuum drying for 3 hours at 55pa of vacuum degree and 48 ℃ to obtain raspberry freeze-dried powder.
And 4, step 4: the freeze-dried rape bee pollen, 133 parts of arginine and 25 parts of crospovidone are uniformly mixed, dried, crushed and granulated to obtain the premix.
And 5: uniformly mixing the premix, raspberry freeze-dried powder and 93 parts of zinc-rich yeast, adding 25 parts of microcrystalline cellulose, 15 parts of hydroxypropyl methylcellulose and 15 parts of magnesium stearate, uniformly mixing, drying, crushing and sieving by a 30-mesh sieve; granulating, drying by air at 60-66 ℃ for 2-3 h, cooling to room temperature, controlling the hardness of the tablets, and tabletting to obtain the product.
Example 6: canola bee pollen was subjected to the same conventional cell wall breaking method as in example 1.
Example 7: the same procedure as in example 1 was repeated except that inorganic zinc was used instead of organic zinc.
Experiment:
a carrageenan-induced non-bacterial prostatitis model was prepared in rats. An incision of about 1cm was made in the center of the lower abdomen of the rat, and 0.05mL of each of newly prepared 1% carrageenan was injected bilaterally into the prostate. Abdominal muscles and skin were sutured after injection. In general, the model was established after 1 week, and was the model group.
A healthy batch of rats was selected as the blank group.
The compositions prepared in examples 1 to 7 were administered to rats infected with nonbacterial prostatitis in the same amount.
The following table shows the results of the organ-related tests of the blank group, the model group and the experimental rats of examples 1 to 7:
and (4) conclusion: as can be seen, the prostate wet weight and prostate index differed greatly among the groups, with the model group rats having significantly increased prostate wet weight and prostate index compared to the blank group rats. The prostate wet weight and prostate index for examples 1-3 were significantly reduced compared to the model group. Examples 4 to 6 all improved, but the effect was not obvious in examples 1 to 3.
The following tables show the urine volume and water intake for the blank group, model group and experimental rats of examples 1-7:
and (4) conclusion: the measurement of urine volume has great reference value for the diagnosis of urinary system diseases, and prostatitis can cause urinary tract infection, and urine volume reduction is caused by difficult urination. As can be seen, the model group had significantly reduced 24-hour urine and water intake compared to the blank group. The urine amount and the water intake of examples 1 to 3 were significantly increased, and the urine amount and the water intake of examples 4 to 5 were smaller than those of examples 6 to 7. The rape bee pollen treated by the pulse electric field wall breaking technology better keeps the nutrient components in the pollen, and the rape bee pollen, the zinc-rich yeast and the aescin are all used for improving the prostatitis.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A method for preparing a composition for preventing and treating prostate diseases, which is characterized by comprising the following steps: the method comprises the following steps:
step 1: removing impurities from rape bee pollen, grinding, preparing rape bee pollen suspension, treating with a pulse electric field, freezing at-18 to-22 ℃ for 24 to 28 hours, adding 80 to 86 ℃ hot water, stirring, cooling to 42 to 46 ℃, continuously stirring at the constant temperature of 44 to 46 ℃ for 6 to 8 hours, freezing, and drying to obtain wall-broken rape bee pollen;
step 2: putting the wall-broken rape bee pollen into a freeze dryer, pre-freezing, sublimating for 2-4 h at the temperature of-40 to-45 ℃, resolving for 2-4 h, vacuum drying for 2-4 h at the vacuum degree of 50-60 pa and the temperature of 46-50 ℃ to obtain freeze-dried rape bee pollen;
and step 3: putting the raspberry into a freeze dryer for pre-freezing, sublimating for 2-4 hours at the temperature of-35 to-40 ℃, resolving for 2-4 hours, and vacuum drying for 2-4 hours at the vacuum degree of 50-60 pa and the temperature of 46-50 ℃ to obtain raspberry freeze-dried powder;
and 4, step 4: mixing lyophilized bee pollen of Brassica campestris, arginine and polyvinylpolypyrrolidone, drying, pulverizing, and granulating to obtain a premix;
and 5: uniformly mixing the premix, raspberry freeze-dried powder, aescin powder and zinc-rich yeast, adding microcrystalline cellulose, hydroxypropyl methylcellulose and magnesium stearate, uniformly mixing, drying, crushing and sieving by a 30-mesh sieve; granulating, drying by air at 60-66 ℃ for 2-3 h, cooling to room temperature, controlling the hardness of the tablets, and tabletting to obtain the product.
2. The method for preparing a composition for preventing and treating prostate diseases according to claim 1, wherein: the composition comprises the following components: 150-160 parts of freeze-dried rape bee pollen, 110-112 parts of raspberry, 90-96 parts of zinc-rich yeast, 130-136 parts of arginine, 10-20 parts of aescin powder, 20-30 parts of crospovidone, 20-30 parts of microcrystalline cellulose, 10-20 parts of hydroxypropyl methylcellulose and 10-20 parts of magnesium stearate.
3. The method for preparing a composition for preventing and treating prostate diseases according to claim 1, wherein: in the step 1, uniformly grinding the rape bee pollen, adding 80% ethanol solution, and stirring for 3-5h to obtain rape bee pollen suspension.
4. The method for preparing a composition for preventing and treating prostate diseases according to claim 1, wherein: in the step 1, while continuously stirring at a constant temperature of 44-46 ℃, warm water at 44-46 ℃ is added once at intervals of 1-2 h.
5. The method for preparing a composition for preventing and treating prostate diseases according to claim 1, wherein: in the step 1, the pulsed electric field treatment is to pump rape bee pollen turbid liquid into a pulsed electric field treatment chamber by a pump, and after air in the treatment chamber and a pipeline is exhausted, the flow rate of the bee pollen liquid is controlled to be 80-90 mL/min; the pulse electric field treatment conditions are as follows: the pulse frequency is 1kHz, the pulse width is 40 mus, the waveform is a bipolar pulse square wave, the electric field intensity is 26.67kV/cm, and the number of processing pulses is respectively 0, 120, 240, 360, 400 and 600.
6. The method for preparing a composition for preventing and treating prostate diseases according to claim 1, wherein: in the step 2, the pre-freezing temperature of the rape bee pollen is-36 to-40 ℃.
7. The method for preparing a composition for preventing and treating prostate diseases according to claim 1, wherein: in the step 3, the pre-freezing temperature of the raspberry is-30 to-36 ℃.
8. The method for preparing a composition for preventing and treating prostate diseases according to claim 1, wherein: in the step 4, the zinc in the zinc-rich yeast is organically synthesized zinc, and comprises any one of zinc gluconate, zinc oxalate, zinc citrate, zinc lactate and zinc glycyrrhetate.
9. The composition for preventing and treating a prostate disease, which is prepared by the method for preparing the composition for preventing and treating a prostate disease according to any one of claims 1 to 8.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117064996A (en) * | 2023-10-08 | 2023-11-17 | 江苏鸿祺生物科技有限公司 | Composition for protecting prostate and preparation method thereof |
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2021
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117064996A (en) * | 2023-10-08 | 2023-11-17 | 江苏鸿祺生物科技有限公司 | Composition for protecting prostate and preparation method thereof |
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Application publication date: 20220111 |