CN113897345A - Complex enzyme preparation for treating rural domestic garbage and preparation method and application thereof - Google Patents

Complex enzyme preparation for treating rural domestic garbage and preparation method and application thereof Download PDF

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Publication number
CN113897345A
CN113897345A CN202111487350.1A CN202111487350A CN113897345A CN 113897345 A CN113897345 A CN 113897345A CN 202111487350 A CN202111487350 A CN 202111487350A CN 113897345 A CN113897345 A CN 113897345A
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parts
enzyme preparation
complex enzyme
rural domestic
garbage
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肖发沂
郭芳先
张彤晨
朱绍辉
齐艳君
王小伟
亓鹏
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Shandong Vocational Animal Science and Veterinary College
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Shandong Vocational Animal Science and Veterinary College
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    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
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    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
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Abstract

The invention belongs to the technical field of garbage treatment and discloses a compound enzyme preparation for treating rural domestic garbage as well as a preparation method and application thereof, wherein the compound enzyme preparation for treating rural domestic garbage specifically comprises the following components in parts by weight: 15-30 parts of cellulase, 10-20 parts of medium-temperature amylase, 7-15 parts of protease, 8-18 parts of glucanase, 10-20 parts of xylanase, 10-25 parts of lipase, 10-20 parts of pectinase and 15-30 parts of a composite enzyme preparation protective solution.

Description

Complex enzyme preparation for treating rural domestic garbage and preparation method and application thereof
Technical Field
The invention belongs to the technical field of garbage treatment, and particularly relates to a complex enzyme preparation for treating rural domestic garbage, and a preparation method and application thereof.
Background
Along with the high-speed development of economy in China, the speed of urbanization is continuously accelerated, the living standard of people is continuously improved, the generation amount of solid waste, particularly urban and rural household garbage is continuously increased, the pollution to the environment is increasingly serious, and the problem of treatment and disposal of the household garbage becomes a hot spot of close concern in government-related departments, environmental protection and environmental sanitation management units, design departments, scientific research institutions, large-scale colleges, industrial communities and the like; the method for treating the domestic garbage with high efficiency and environmental protection is a problem to be solved urgently in the sustainable development of the domestic ecological environment at present.
At present, domestic garbage treatment modes widely adopted at home and abroad mainly comprise: sanitary landfill, composting method, burning method, fermentation biogas production method; today, the site selection of a landfill site is more and more difficult, the sanitary landfill treatment occupies a large amount of land resources, the land occupation time of the landfill site is long, the land resources are not favorably recycled, and a large amount of percolate, landfill gas and garbage floating dust are generated in the garbage landfill process to pollute the environment.
The most main problem faced by the waste incineration is the generation of waste incineration tail gas containing particulate matters and SO2、SO3NOX, HCl, HF, heavy metals, dioxins and other pollutantsAmong the most interesting and controversial are dioxins; particularly, in China, the domestic garbage has high moisture and ash content and low heat value, needs more auxiliary fuel, and except a few cities with developed economy, the mixed garbage in other cities has low heat value and high slag content, and the incineration fly ash is not safely disposed.
The composting method is to utilize microorganisms in the nature to degrade organic matters in the garbage to change the organic matters into stable humus which can be used as fertilizer; however, the method has the disadvantages of slow temperature rise, long treatment time, incomplete fermentation, incapability of effectively killing harmful pathogenic bacteria in the household garbage, release of harmful substances with foul odor such as ammonia, amine salt, sulfide and the like, large odor and environmental pollution.
Disclosure of Invention
The invention provides a complex enzyme preparation for treating rural domestic garbage, and a preparation method and application thereof, and the complex enzyme preparation has high-efficiency specific catalytic activity, can quickly decompose macromolecular substances such as cellulose, starch, protein, fat, pectin and the like in rural domestic garbage (mainly organic garbage such as leftovers, fruit peels, vegetable leaves, straws and the like), is low in use and production cost, is convenient to use, and can quickly treat the rural domestic garbage.
In order to solve the technical problems, the invention provides the following technical scheme:
a complex enzyme preparation for treating rural domestic garbage comprises the following components in parts by weight: 15-30 parts of cellulase, 10-20 parts of medium-temperature amylase, 7-15 parts of protease, 8-18 parts of glucanase, 10-20 parts of xylanase, 10-25 parts of lipase, 10-20 parts of pectinase and 15-30 parts of complex enzyme preparation protective solution.
The following is a further optimization of the above technical solution of the present invention:
after blending, the activity of each single enzyme in the stock solution of the complex enzyme preparation is as follows: 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucanase, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
Further optimization: the compound enzyme preparation protective solution specifically comprises the following components in parts by weight: 30-50 parts of water, 20-40 parts of glycerol, 10-20 parts of sodium chloride, 5-15 parts of cane sugar and 20-30 parts of amino acid.
The invention also provides a preparation method of the complex enzyme preparation for treating the rural domestic garbage, which specifically comprises the following steps:
s1, preparation of complex enzyme: diluting liquid preparations of cellulase, moderate temperature amylase, protease, glucanase, xylanase, lipase and pectinase with standard enzyme activity to the required enzyme activity;
s2, preparing a compound enzyme preparation protective solution: adopting water as a matrix, dissolving sodium chloride, cane sugar and amino acid in sequence, and then adding glycerol for mixing;
s3, preparing a complex enzyme preparation.
The following is a further optimization of the above technical solution of the present invention:
further optimization: the enzyme activities required by the single enzyme liquids in the step S1 are 10000U/ml of cellulase, 2000U/ml of medium temperature amylase, 5000U/ml of protease, 2000U/ml of glucan, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
Further optimization: the preparation of the compound enzyme preparation protective solution in the step S2 is carried out according to the following steps:
(1) taking a clean container, weighing 30-50 parts of water and 10-20 parts of sodium chloride according to parts by weight, pouring into the container, and stirring to completely dissolve the sodium chloride to obtain solution A;
(2) weighing 5-15 parts of sucrose according to parts by weight, adding the sucrose into the solution A, and stirring to completely dissolve the sucrose to obtain a solution B;
(3) weighing 20-30 parts of amino acid according to parts by weight, adding the amino acid into the solution B, and stirring to completely dissolve the amino acid to obtain solution C;
(4) and weighing 20-40 parts of glycerol according to parts by weight, adding the glycerol into the solution C, and uniformly stirring to obtain the compound enzyme preparation protective solution.
Further optimization: the preparation of the complex enzyme preparation in the step S3 is carried out according to the following steps:
1) respectively weighing the following raw materials in parts by weight according to the enzyme activity of each single enzyme: 15-30 parts of cellulase, 10-20 parts of medium-temperature amylase, 7-15 parts of protease, 8-18 parts of glucanase, 10-20 parts of xylanase, 10-25 parts of lipase and 10-20 parts of pectinase;
2) weighing 15-30 parts of a composite enzyme preparation protective solution according to parts by weight;
3) and adding the raw materials weighed in the step 1) and the step 2) into a stirring tank, stirring and mixing uniformly to finally obtain the complex enzyme preparation for treating the rural domestic garbage.
The invention also provides an application of the complex enzyme preparation for treating the rural domestic garbage, which is based on the complex enzyme preparation for treating the rural domestic garbage; the application is carried out according to the following steps:
y1, firstly, putting the organic garbage in the sorted rural domestic garbage into a garbage disposer for crushing, adding a complex enzyme preparation for treating the rural domestic garbage into the garbage disposer, and stirring;
y2, then raising the temperature inside the garbage disposer to 37-43 ℃, reacting for 1.5-3.5 hours, then raising the temperature to 48-55 ℃, reacting for 2-4 hours, then raising the temperature to 58-63 ℃, reacting for 2-4 hours, finally raising the temperature to 100 ℃, and treating for 45-60 minutes.
The following is a further optimization of the above technical solution of the present invention:
in the step Y1, the adding amount of the complex enzyme preparation for treating the rural domestic garbage is 5-10% of the weight of the organic garbage.
Further optimization: the whole garbage treatment time of the step Y1 and the step Y2 is as follows: 7-13 hours.
By adopting the technical scheme, the activity of each single enzyme in the stock solution of the blended complex enzyme preparation is as follows: 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucanase, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase; and the high-efficiency specific catalytic activity of each single enzyme is utilized, so that macromolecular substances such as cellulose, starch, protein, fat, pectin and the like in rural domestic garbage (mainly organic garbage such as leftovers, fruit peels, vegetable leaves, straws and the like) can be treated and converted into micromolecular substances which can be easily absorbed and utilized by plants, and the micromolecular substances can be directly applied to fields after the treatment and used as organic fertilizers.
When the compound enzyme preparation for treating the rural domestic garbage is used for treating the rural domestic garbage, the treatment can be completed within 7-13 hours, compared with the prior art, the treatment time is short, the treatment speed and the treatment effect are improved, organic matters of the materials are basically unchanged after the treatment is completed, and the volume is obviously reduced; harmful bacteria such as worm eggs, escherichia coli, salmonella, faecal coliform and the like are not detected.
The invention is used for treating rural household garbage, has low production cost, convenient use, environmental protection and high treatment effect compared with the prior art, and can realize the purpose of changing waste into valuable.
The present invention will be further described with reference to the following examples.
Detailed Description
Example 1:
a complex enzyme preparation for treating rural domestic garbage mainly comprises cellulase, medium-temperature amylase, protease, glucanase, xylanase, lipase, pectinase and a complex enzyme preparation protective solution.
The components are as follows according to the weight portion: 15 parts of cellulase, 10 parts of medium-temperature amylase, 7 parts of protease, 8 parts of glucanase, 10 parts of xylanase, 10 parts of lipase, 10 parts of pectinase and 15 parts of complex enzyme preparation protective solution.
The activity of each single enzyme in the stock solution of the blended complex enzyme preparation is as follows: 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucanase, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
The composite enzyme preparation protective solution comprises the following main raw materials: water, glycerol, sodium chloride, sucrose, amino acids.
The compound enzyme preparation protective solution comprises the following components in parts by weight: 30 parts of water, 20 parts of glycerol, 10 parts of sodium chloride, 5 parts of cane sugar and 20 parts of amino acid.
In the embodiment, the preparation method of the complex enzyme preparation for treating the rural domestic garbage is further provided, and the preparation method comprises the following steps of S1, preparing the complex enzyme, and the step S1 is carried out according to the following steps:
firstly, determining enzyme activity required by each single enzyme according to the content of each component in rural domestic garbage (mainly including organic garbage such as leftovers, fruit peels, vegetable leaves, straws and the like).
The enzyme activity required by each single enzyme liquid is 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucan, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
Then, a commercially available single-enzyme liquid preparation with standard enzyme activity is diluted to the required enzyme activity.
The method further comprises the following steps: s2, preparing a compound enzyme preparation protective solution, adopting water as a matrix, dissolving sodium chloride, cane sugar and amino acid in sequence, and then adding glycerol for mixing.
The preparation of the compound enzyme preparation protective solution in the step S2 is carried out according to the following steps:
(1) and weighing 30 parts of water and 10 parts of sodium chloride in a clean container respectively according to the parts by weight, and pouring the water and the sodium chloride into the container to be stirred so as to completely dissolve the sodium chloride to obtain the solution A.
(2) And then 5 parts of sucrose is weighed according to the parts by weight and added into the solution A, and then the sucrose is completely dissolved by stirring to obtain solution B.
(3) And weighing 20 parts of amino acid according to the parts by weight, adding the amino acid into the solution B, and stirring to completely dissolve the amino acid to obtain solution C.
4. And finally, weighing 20 parts of glycerol according to the parts by weight, adding the glycerol into the solution C, and uniformly stirring to obtain the compound enzyme preparation protective solution.
The components and the proportion in the compound enzyme preparation protective solution are the optimal proportion obtained through multiple experimental verification, and after the compound enzyme preparation protective solution is used, the enzyme activity of each single enzyme of the compound enzyme preparation for treating the rural domestic garbage basically has no loss after the compound enzyme preparation is placed in a room temperature environment for one year, so that the service life of the compound enzyme preparation for treating the rural domestic garbage can be prolonged, and the use effect is improved.
The method further comprises the following steps: s3, preparing a complex enzyme preparation; the preparation of the complex enzyme preparation is carried out according to the following steps:
1) respectively weighing the following raw materials in parts by weight according to the enzyme activity of each single enzyme: 15 parts of cellulase, 10 parts of medium temperature amylase, 7 parts of protease, 8 parts of glucanase, 10 parts of xylanase, 10 parts of lipase and 10 parts of pectinase.
2) And weighing 15 parts of the compound enzyme preparation protective solution according to the parts by weight.
3) And adding the raw materials weighed in the step 1) and the step 2) into a stirring tank for stirring, so that the raw materials are fully and uniformly mixed, and finally obtaining the complex enzyme preparation for treating the rural domestic garbage.
The preparation method of the complex enzyme preparation for treating the rural domestic garbage disclosed by the invention has the advantages that the process is simple, the production is convenient, the production cost can be greatly reduced, the use effect is improved, the complex enzyme preparation protective solution is added into the complex enzyme preparation for treating the rural domestic garbage, the enzyme activity of each single enzyme of the complex enzyme preparation for treating the rural domestic garbage is basically not lost after the complex enzyme preparation for treating the rural domestic garbage is placed for one year in a room temperature environment through the complex enzyme preparation protective solution, the service life of the complex enzyme preparation for treating the rural domestic garbage can be prolonged, and the use effect is improved.
In the embodiment, the application of the complex enzyme preparation for treating the rural domestic garbage is further provided, and the application is carried out according to the following steps:
y1, firstly, putting the organic garbage in the sorted rural domestic garbage into a garbage disposer for crushing, adding a complex enzyme preparation for treating the rural domestic garbage into the garbage disposer, and stirring, wherein the adding amount of the complex enzyme preparation for treating the rural domestic garbage is 5% of the weight of the organic garbage in the garbage disposer.
In the step Y1, the compound enzyme preparation for treating the rural domestic garbage is added into the garbage disposer in a spraying mode, and the compound enzyme preparation for treating the rural domestic garbage is sprayed and stirred while being smashed along with the garbage disposer, so that the enzyme preparation and the garbage raw materials are fully mixed.
By the design, the complex enzyme preparation for treating the rural domestic garbage and the garbage raw materials can be fully mixed, so that the mixing effect is improved, and the subsequent treatment at the later stage is facilitated.
Y2, then raising the temperature inside the garbage disposer to 37 ℃, reacting for 1.5 hours, then raising the temperature to 48 ℃, reacting for 2 hours, then raising the temperature to 58 ℃, reacting for 2 hours, finally raising the temperature to 100 ℃, and treating for 45 minutes.
Stirring is started all the time in the whole temperature rising reaction process to kill harmful microorganisms and worm eggs in the garbage, and the raw materials are discharged after the temperature is cooled to the room temperature and can be directly applied to the field to be used as organic fertilizer.
The whole garbage treatment time is as follows: for 7 hours.
Example 2:
a complex enzyme preparation for treating rural domestic garbage comprises the following components in parts by weight: 23 parts of cellulase, 15 parts of medium-temperature amylase, 11 parts of protease, 13 parts of glucanase, 15 parts of xylanase, 18 parts of lipase, 15 parts of pectinase and 23 parts of complex enzyme preparation protective solution.
The enzyme activity of the stock solution of the blended complex enzyme preparation is as follows: 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucanase, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
The compound enzyme preparation protective solution comprises the following components in parts by weight: 40 parts of water, 30 parts of glycerol, 15 parts of sodium chloride, 10 parts of cane sugar and 25 parts of amino acid.
In the embodiment, the preparation method of the complex enzyme preparation for treating the rural domestic garbage is also provided, and the preparation method comprises the steps of S1 and preparation of the complex enzyme; the preparation of the complex enzyme is carried out according to the following steps:
the single enzyme liquid preparation with standard enzyme activity purchased from the market is diluted until the enzyme activity is as follows: 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucan, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
The method further comprises the following steps: s2, preparing a compound enzyme preparation protective solution, wherein the compound enzyme preparation protective solution is prepared according to the following steps:
(1) and taking a clean container, weighing 40 parts of water and 15 parts of sodium chloride in sequence respectively, and pouring the water into the container to stir so as to completely dissolve the sodium chloride to obtain the solution A.
(2) Then 10 parts of sucrose is weighed and added into the solution A, and then the sucrose is completely dissolved by stirring to obtain solution B.
(3) And weighing 25 parts of amino acid, adding the amino acid into the solution B, and stirring to completely dissolve the amino acid to obtain solution C.
(4) And finally weighing 30 parts of glycerol, adding the glycerol into the solution C, and uniformly stirring to obtain the compound enzyme preparation protective solution.
The method further comprises the following steps: s3, preparing a complex enzyme preparation; the preparation of the complex enzyme preparation is carried out according to the following steps:
1) respectively weighing the following raw materials in parts by weight according to the enzyme activity of each single enzyme: 23 parts of cellulase, 15 parts of medium temperature amylase, 11 parts of protease, 13 parts of glucanase, 15 parts of xylanase, 18 parts of lipase and 15 parts of pectinase.
2) And weighing 23 parts of the compound enzyme preparation protective solution according to the parts by weight.
3) And adding the raw materials weighed in the step 1) and the step 2) into a stirring tank for stirring, so that the raw materials are fully and uniformly mixed, and finally obtaining the complex enzyme preparation for treating the rural domestic garbage.
In the embodiment, the application of the complex enzyme preparation for treating the rural domestic garbage is further provided, and the application is carried out according to the following steps:
y1, firstly, putting the organic garbage in the sorted rural domestic garbage into a garbage disposer for crushing, adding 8% of complex enzyme preparation for treating the rural domestic garbage into the garbage disposer by weight, and starting stirring.
In the step Y1, the compound enzyme preparation for treating the rural domestic garbage is added into the garbage disposer in a spraying mode, and the compound enzyme preparation for treating the rural domestic garbage is sprayed and stirred while being smashed along with the garbage disposer, so that the enzyme preparation and the garbage raw materials are fully mixed.
By the design, the complex enzyme preparation for treating the rural domestic garbage and the garbage raw materials can be fully mixed, so that the mixing effect is improved, and the subsequent treatment at the later stage is facilitated.
Y2, then raising the temperature inside the garbage disposer to 40 ℃, reacting for 2.5 hours, then raising the temperature to 52 ℃, reacting for 3 hours, then raising the temperature to 61 ℃, reacting for 3 hours, finally raising the temperature to 100 ℃, and treating for 51 minutes.
Stirring is started all the time in the whole temperature rising reaction process to kill harmful microorganisms and worm eggs in the garbage, and the raw materials are discharged after the temperature is cooled to the room temperature and can be directly applied to the field to be used as organic fertilizer.
The whole garbage treatment time is as follows: for 10 hours.
Example 3:
a complex enzyme preparation for treating rural domestic garbage comprises the following components in parts by weight: 30 parts of cellulase, 20 parts of medium-temperature amylase, 15 parts of protease, 18 parts of glucanase, 20 parts of xylanase, 25 parts of lipase, 20 parts of pectinase and 30 parts of complex enzyme preparation protective solution
The enzyme activity of the stock solution of the blended complex enzyme preparation is as follows: 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucanase, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
The compound enzyme preparation protective solution comprises the following components in parts by weight: 50 parts of water, 40 parts of glycerol, 20 parts of sodium chloride, 15 parts of cane sugar and 30 parts of amino acid.
In the embodiment, the preparation method of the complex enzyme preparation for treating the rural domestic garbage is also provided, and the preparation method comprises the steps of S1 and preparation of the complex enzyme; the preparation of the complex enzyme is carried out according to the following steps:
the single enzyme liquid preparation with standard enzyme activity purchased from the market is diluted until the enzyme activity is as follows: 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucan, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
The method further comprises the following steps: s2, preparing a compound enzyme preparation protective solution, wherein the compound enzyme preparation protective solution is prepared according to the following steps:
(1) and taking a clean container, weighing 50 parts of water and 20 parts of sodium chloride respectively in sequence, and pouring the water and the sodium chloride into the container to be stirred so as to completely dissolve the sodium chloride to obtain the solution A.
(2) Then 15 parts of sucrose is weighed and added into the solution A, and then the sucrose is completely dissolved by stirring to obtain solution B.
(3) And weighing 30 parts of amino acid, adding the amino acid into the solution B, and stirring to completely dissolve the amino acid to obtain solution C.
(4) And finally, weighing 40 parts of glycerol, adding the glycerol into the solution C, and uniformly stirring to obtain the compound enzyme preparation protective solution.
The method further comprises the following steps: s3, preparing a complex enzyme preparation; the preparation of the complex enzyme preparation is carried out according to the following steps:
1) respectively weighing the following raw materials in parts by weight according to the enzyme activity of each single enzyme: 30 parts of cellulase, 20 parts of medium temperature amylase, 15 parts of protease, 18 parts of glucanase, 20 parts of xylanase, 25 parts of lipase and 20 parts of pectinase.
2) And weighing 30 parts of the compound enzyme preparation protective solution according to the parts by weight.
3) And adding the raw materials weighed in the step 1) and the step 2) into a stirring tank for stirring, so that the raw materials are fully and uniformly mixed, and finally obtaining the complex enzyme preparation for treating the rural domestic garbage.
In the embodiment, the application of the complex enzyme preparation for treating the rural domestic garbage is further provided, and the application is carried out according to the following steps:
y1, firstly, putting the organic garbage in the sorted rural domestic garbage into a garbage disposer for crushing, adding 10% of complex enzyme preparation for treating the rural domestic garbage into the garbage disposer by weight, and starting stirring.
In the step Y1, the compound enzyme preparation for treating the rural domestic garbage is added into the garbage disposer in a spraying mode, and the compound enzyme preparation for treating the rural domestic garbage is sprayed and stirred while being smashed along with the garbage disposer, so that the enzyme preparation and the garbage raw materials are fully mixed.
By the design, the complex enzyme preparation for treating the rural domestic garbage and the garbage raw materials can be fully mixed, so that the mixing effect is improved, and the subsequent treatment at the later stage is facilitated.
Y2, then raising the temperature inside the garbage disposer to 43 ℃, reacting for 3.5 hours, then raising the temperature to 55 ℃, reacting for 4 hours, then raising the temperature to 63 ℃, reacting for 4 hours, finally raising the temperature to 100 ℃, and treating for 60 minutes.
Stirring is started all the time in the whole temperature rising reaction process to kill harmful microorganisms and worm eggs in the garbage, and the raw materials are discharged after the temperature is cooled to the room temperature and can be directly applied to the field to be used as organic fertilizer.
The whole garbage treatment time is as follows: for 13 hours.
The compound enzyme preparation for treating the rural domestic garbage and the application method thereof are adopted to treat the rural domestic garbage, wherein the rural domestic garbage mainly refers to organic garbage such as leftovers, fruit peels, vegetable leaves, straws and the like, and the rural domestic garbage contains a large amount of macromolecular substances such as cellulose, starch, protein, fat, pectin and the like.
After the treatment is finished, the treated rural domestic garbage is detected, and the detection structure is shown in the following table:
Figure 412557DEST_PATH_IMAGE001
as can be seen from the above table, after the rural domestic garbage is treated by the complex enzyme preparation disclosed by the invention, no pericarp, vegetable leaves, straw and the like can be seen, organic matters of the materials are basically unchanged, the volume is obviously reduced, and harmful bacteria such as worm eggs, escherichia coli, salmonella, faecal coliform and the like are not detected.
Compared with the method for treating the garbage by utilizing the microbial fermentation, the method has the advantages that the treatment time is short, and the organic matters are far higher than those of the garbage treated by the microbial fermentation.
It will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in the embodiments described above without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims.

Claims (10)

1. A complex enzyme preparation for treating rural domestic garbage is characterized in that: the composition specifically comprises the following components in parts by weight: 15-30 parts of cellulase, 10-20 parts of medium-temperature amylase, 7-15 parts of protease, 8-18 parts of glucanase, 10-20 parts of xylanase, 10-25 parts of lipase, 10-20 parts of pectinase and 15-30 parts of complex enzyme preparation protective solution.
2. The complex enzyme preparation for treating rural domestic waste according to claim 1, which is characterized in that: after blending, the activity of each single enzyme in the stock solution of the complex enzyme preparation is as follows: 10000U/ml of cellulase, 2000U/ml of mesophilic amylase, 5000U/ml of protease, 2000U/ml of glucanase, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
3. The complex enzyme preparation for treating rural domestic waste according to claim 2, which is characterized in that: the compound enzyme preparation protective solution specifically comprises the following components in parts by weight: 30-50 parts of water, 20-40 parts of glycerol, 10-20 parts of sodium chloride, 5-15 parts of cane sugar and 20-30 parts of amino acid.
4. The method for preparing the complex enzyme preparation for treating rural domestic garbage according to any one of claims 1 to 3, which is characterized in that: the preparation method specifically comprises the following steps:
s1, preparation of complex enzyme: diluting liquid preparations of cellulase, moderate temperature amylase, protease, glucanase, xylanase, lipase and pectinase with standard enzyme activity to the required enzyme activity;
s2, preparing a compound enzyme preparation protective solution: adopting water as a matrix, dissolving sodium chloride, cane sugar and amino acid in sequence, and then adding glycerol for mixing;
s3, preparing a complex enzyme preparation.
5. The method for preparing the complex enzyme preparation for treating rural domestic garbage according to claim 4, which is characterized by comprising the following steps: the enzyme activities required by the single enzyme liquids in the step S1 are 10000U/ml of cellulase, 2000U/ml of medium temperature amylase, 5000U/ml of protease, 2000U/ml of glucan, 5000U/ml of xylanase, 3000U/ml of lipase and 1500U/ml of pectinase.
6. The method for preparing the complex enzyme preparation for treating rural domestic garbage according to claim 5, which is characterized by comprising the following steps: the preparation of the compound enzyme preparation protective solution in the step S2 is carried out according to the following steps:
(1) taking a clean container, weighing 30-50 parts of water and 10-20 parts of sodium chloride according to parts by weight, pouring into the container, and stirring to completely dissolve the sodium chloride to obtain solution A;
(2) weighing 5-15 parts of sucrose according to parts by weight, adding the sucrose into the solution A, and stirring to completely dissolve the sucrose to obtain a solution B;
(3) weighing 20-30 parts of amino acid according to parts by weight, adding the amino acid into the solution B, and stirring to completely dissolve the amino acid to obtain solution C;
(4) and weighing 20-40 parts of glycerol according to parts by weight, adding the glycerol into the solution C, and uniformly stirring to obtain the compound enzyme preparation protective solution.
7. The method for preparing the complex enzyme preparation for treating rural domestic garbage according to claim 6, which is characterized in that: the preparation of the complex enzyme preparation in the step S3 is carried out according to the following steps:
1) respectively weighing the following raw materials in parts by weight according to the enzyme activity of each single enzyme: 15-30 parts of cellulase, 10-20 parts of medium-temperature amylase, 7-15 parts of protease, 8-18 parts of glucanase, 10-20 parts of xylanase, 10-25 parts of lipase and 10-20 parts of pectinase;
2) weighing 15-30 parts of a composite enzyme preparation protective solution according to parts by weight;
3) and adding the raw materials weighed in the step 1) and the step 2) into a stirring tank, stirring and mixing uniformly to finally obtain the complex enzyme preparation for treating the rural domestic garbage.
8. The application of the complex enzyme preparation for treating rural domestic garbage based on any one of claims 1-3 is characterized in that: the application is carried out according to the following steps:
y1, firstly, putting the organic garbage in the sorted rural domestic garbage into a garbage disposer for crushing, adding a complex enzyme preparation for treating the rural domestic garbage into the garbage disposer, and stirring;
y2, then raising the temperature inside the garbage disposer to 37-43 ℃, reacting for 1.5-3.5 hours, then raising the temperature to 48-55 ℃, reacting for 2-4 hours, then raising the temperature to 58-63 ℃, reacting for 2-4 hours, finally raising the temperature to 100 ℃, and treating for 45-60 minutes.
9. The application of the complex enzyme preparation for treating rural domestic garbage according to claim 8, wherein the complex enzyme preparation comprises the following components: in the step Y1, the adding amount of the complex enzyme preparation for treating the rural domestic garbage is 5-10% of the weight of the organic garbage.
10. The application of the complex enzyme preparation for treating rural domestic garbage according to claim 9, wherein the complex enzyme preparation comprises the following components: the whole garbage treatment time of the step Y1 and the step Y2 is as follows: 7-13 hours.
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