CN113892567A - Ingredients and preparation method of liver and intestine health-care natural plant fermented feed for exopalaemon carinicauda - Google Patents
Ingredients and preparation method of liver and intestine health-care natural plant fermented feed for exopalaemon carinicauda Download PDFInfo
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Abstract
The ingredients and the preparation method of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda comprise the following steps: fermentation substrate and zymogen liquid; the fermentation substrate comprises corn, soybean meal, rhizoma atractylodis, ligusticum wallichii, mangnolia officinalis, Chinese yam, radix paeoniae alba, eucommia ulmoides and bran; the corn, the bean pulp, the rhizoma atractylodis, the ligusticum wallichii, the mangnolia officinalis, the Chinese yam, the white paeony root and the eucommia ulmoides are ground into 30-40 meshes, and then mixed with 30-40 meshes of bran to prepare the fermentation substrate. The method effectively overcomes the defect that the symptoms are not the root cause of the disease in the prior art by preventing and treating the enteritis and the hepatobiliary diseases of the exopalaemon carinicauda by mixing and feeding the allicin.
Description
Technical Field
The invention relates to the technical field of fermented feeds, in particular to a formula and a preparation method of a liver and intestine health-care natural plant fermented feed for exopalaemon carinicauda.
Background
Exopalaemon carinicauda is a shrimp belonging to the genus Penaeus of the family Pandalidae. The body length is 50-90 mm. The forehead angle is flat, the length of the forehead angle is 1.2-1.5 times of that of the skull, the cockscomb-shaped projection at the base part is shorter than the tip part of the tail part, and the tail part is raised upwards. The palpation is very small and the gill nail is large. The front edge of the base section of the first antenna handle is round, and the front side prick is small. The back of the third section to the sixth section of the abdomen is provided with obvious longitudinal ridges. The palaemon carinicauda has euryhaline property, a culture area is mainly distributed along the coastwise of yellow and Bohai sea in China, the palaemon carinicauda has the characteristics of low temperature resistance, low oxygen and low salt, quick growth, delicious taste, stable price, remarkable culture benefit and the like, and is limited by a culture area.
At present, allicin is mainly fed for prevention and treatment, the symptoms are treated but not the root causes, the sustainable development of the palaemon carinicauda breeding industry is troubled by the problems caused by the allicin, and the biological fermentation technology is urgently needed to be utilized to develop a liver and intestine health care type feedable natural plant fermented feed for relieving the current breeding problems.
Disclosure of Invention
In order to solve the problems, the invention provides ingredients and a preparation method of a liver and intestine health-care natural plant fermented feed for exopalaemon carinicauda, which effectively overcome the defect that the symptoms are treated but not the root causes by preventing and treating enteritis and hepatobiliary diseases of exopalaemon carinicauda in the prior art by feeding allicin.
In order to overcome the defects in the prior art, the invention provides a solution of ingredients and a preparation method of a health-care natural plant fermented feed for the liver and intestine of exopalaemon carinicauda, which comprises the following steps:
a formula of a liver and intestine health-care natural plant fermented feed for exopalaemon carinicauda comprises:
fermentation substrate and zymogen liquid;
the fermentation substrate comprises corn, soybean meal, rhizoma atractylodis, ligusticum wallichii, mangnolia officinalis, Chinese yam, radix paeoniae alba, eucommia ulmoides and bran;
the corn, the bean pulp, the rhizoma atractylodis, the ligusticum wallichii, the mangnolia officinalis, the Chinese yam, the white paeony root and the eucommia ulmoides are ground into 30-40 meshes, and then mixed with 30-40 meshes of bran to prepare the fermentation substrate.
Further, the corn is 40-45 parts by weight, the soybean meal is 20-25 parts by weight, the bran is 15-20 parts by weight, the rhizoma atractylodis is 10-15 parts by weight, the ligusticum wallichii is 12-18 parts by weight, the mangnolia officinalis is 15-20 parts by weight, the Chinese yam is 20-25 parts by weight, the radix paeoniae alba is 15-20 parts by weight, and the eucommia ulmoides is 13-18 parts by weight.
Further, the raw materials of the zymocyte liquid comprise: lactobacillus casei, lactobacillus plantarum, bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, brown sugar and water;
the strain ratio of the lactobacillus casei, the lactobacillus plantarum, the bacillus subtilis, the bacillus licheniformis and the saccharomyces cerevisiae is as follows: lactobacillus casei: lactobacillus plantarum: b, bacillus subtilis: b, bacillus licheniformis: saccharomyces cerevisiae ═ (3-7): (2-4): (3-7): (2-4): (4-6);
sealing and activating lactobacillus casei, lactobacillus plantarum, brown sugar and water for 3-5 hours to prepare activated bacterium liquid I for later use, wherein the weight ratio of the lactobacillus casei to the lactobacillus plantarum to the brown sugar to the water is as follows: lactobacillus casei and lactobacillus plantarum: brown sugar: water ═ 5-15: (2-8): (40-120);
b, oxygenating and activating the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the brown sugar and water for 2-3 hours to prepare an activated bacterium solution II for later use, wherein the weight ratio of the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the brown sugar to the water is as follows: bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae: brown sugar: water ═ 5-25: (2-8): (40-120);
mixing the activated bacterium liquid I and the activated bacterium liquid II to obtain a mixture, adding water into the mixture to prepare a zymogen liquid, wherein the weight ratio of the activated bacterium liquid I to the activated bacterium liquid II is (3-9): (2-6), wherein the weight ratio of the mixture to the water is 1: (80-100).
Further, the weight ratio of the zymocyte liquid to the fermentation substrate is as follows: (300-400): 1000.
a preparation method of a liver and intestine health-care natural plant fermented feed for exopalaemon carinicauda comprises the following steps:
step 1: uniformly mixing a fermentation substrate and a zymogen liquid to prepare a fermentation material;
step 2: stacking the fermented materials in a fermentation tank, wherein the height of the fermented materials is 60-80 cm;
and step 3: the fermented materials are periodically turned over by monitoring the change of the temperature and the pH value of the fermented materials, so that aerobic fermentation and anaerobic fermentation are alternately performed, wherein the fermentation periods of the aerobic fermentation and the anaerobic fermentation are both 80-120 h, and the finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda is obtained.
Further, the manner of monitoring the temperature and pH changes of the fermentation material includes: an electronic thermometer is arranged in the fermentation tank for temperature monitoring, the temperature is recorded once every two hours, and the rake is turned when the temperature is higher than 47 ℃, so that the aerobic fermentation and the anaerobic fermentation are alternately carried out;
simultaneously monitoring the pH value, wherein the method comprises the following steps: and (4) carrying out pH value detection on the fermented material every 18 hours, and obtaining a finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda when the pH value is less than 4.5.
The invention has the beneficial effects that:
1) the problem of frozen fresh feed quality stability is solved: the fermented feed is rich in metabolites such as various organic acids, enzymes and bacteriocins and beneficial bacteria, is uniformly mixed with the icy fresh feed according to the proportion of 20-30%, can effectively inhibit the breeding and propagation of harmful microorganisms in the icy fresh feed, prevents putrefaction and deterioration, and achieves the effect of continuous preservation.
2) Solves the problems of liver transformation and enteritis of the exopalaemon carinicauda; the rhizoma atractylodis, the ligusticum wallichii, the mangnolia officinalis, the Chinese yam, the white paeony root and the eucommia bark can be used as feeding natural plants, and the rhizoma atractylodis is warm in nature, pungent and bitter in taste. The product has effects of eliminating dampness, invigorating spleen, dispelling pathogenic wind, dispelling cold, removing liver fire, and improving eyesight, and comprises atractylone, 2-carene, atractylenolide, patchouli, elemene, acetyl xanthinol, dehydroxylin ester, and atractylone, atractylone spironols as main ingredients in the volatile oil. Chuan Xiong is warm in nature and pungent in flavor. The Chinese medicine preparation is prepared with volatile oil in 40 weight portions accounting for 93.64 wt%, ligustilide in 58 wt%, 3-butylidenephthalide in 5.29 wt% and sabinene in 6.08 wt%, and ferulic acid, indole, 3-butylidenephthalide, myrcene, ligustrazine, palmitic acid, daucosterol, beta-sitosterol, etc. and has the functions of promoting blood circulation and promoting circulation of qi. Magnolia bark is warm in nature, bitter and pungent in taste. It is effective in eliminating dampness and phlegm, descending qi and eliminating fullness, and comprises volatile oil, alkaloid, saponin, tannin, and trace amount of nicotinic acid. Shan Yao is mild in nature and sweet in taste. Spleen channels, lung channels and kidney channels, and has effects of invigorating spleen and stomach, promoting fluid production, benefiting lung, invigorating kidney, and arresting seminal emission, and contains dopamine, dioscine, maple II, stigmasterol, cholesterol, dioscine hydrochloride, ergosterol, quinic acid, campesterol, cholesterol, diosgenin, allantoin, campesterol, hexacosanic acid, -sitosterol, free amino acids, starch, tannin, mucus, glycoprotein, and polyphenol oxidase. White peony root is slightly cold in nature, bitter and sour in taste. Belongs to the liver channel and the spleen channel, calms the liver, relieves pain and nourishes blood, and contains paeoniflorin, hydroxyl paeoniflorin, benzoylpaeoniflorin, albiflorin, oxypaeoniflorin, paeoniflorin, hederagenin, paeoniflorin ketone, galloyl paeoniflorin, kaempferol-3, 7-di-O-beta-D-glucoside, daucosterol, beta-sitosterol, paeoniflorin and the like, and the volatile oil mainly contains benzoic acid, paeonol and the like. Eucommia bark is warm in nature and sweet in taste. Enter liver and kidney meridians, nourish liver and kidney, strengthen tendons and bones, and contain pinoresinol diglucoside, D-eugenol, eucommia ulmoides glycosides, eucommia ulmoides oliv A, erythro-dihydroxy dehydro-dibastinol, etc. Recently, the branch bark contains nonacosane, octacosanol, beta-sitosterol, betulin, betulinic acid and ursolic acid.
Effectively avoids the defect that the allicin is mixed and fed to prevent and treat the enteritis and the liver and gall diseases of the exopalaemon carinicauda in the prior art to treat the symptoms and the root causes.
Drawings
FIG. 1 is a flow chart of the preparation method of the present invention.
Detailed Description
The invention will be further described with reference to the following figures and examples.
Example 1:
as shown in figure 1, the formula of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda comprises the following components:
fermentation substrate and zymogen liquid;
the fermentation substrate comprises corn, soybean meal, rhizoma atractylodis, ligusticum wallichii, mangnolia officinalis, Chinese yam, radix paeoniae alba, eucommia ulmoides and bran;
the corn, the bean pulp, the rhizoma atractylodis, the ligusticum wallichii, the mangnolia officinalis, the Chinese yam, the white paeony root and the eucommia ulmoides are crushed to 30 meshes and then mixed with 30 meshes of bran to prepare a fermentation substrate.
The weight parts of the corn are 40 parts, the weight parts of the soybean meal are 20 parts, the weight parts of the bran are 15 parts, the weight parts of the rhizoma atractylodis are 10 parts, the weight parts of the ligusticum wallichii are 12 parts, the weight parts of the magnolia officinalis are 15 parts, the weight parts of the Chinese yam are 20 parts, the weight parts of the white paeony root are 15 parts, and the weight parts of the eucommia ulmoides are 13 parts.
The raw materials of the zymocyte liquid comprise: lactobacillus casei, lactobacillus plantarum, bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, brown sugar and water;
the strain ratio of the lactobacillus casei, the lactobacillus plantarum, the bacillus subtilis, the bacillus licheniformis and the saccharomyces cerevisiae is as follows: lactobacillus casei: lactobacillus plantarum: b, bacillus subtilis: b, bacillus licheniformis: 3, saccharomyces cerevisiae: 2: 3: 2: 4;
sealing and activating lactobacillus casei, lactobacillus plantarum, brown sugar and water for 3 hours to prepare activated bacterium liquid I for later use, wherein the weight ratio of the lactobacillus casei to the lactobacillus plantarum to the brown sugar to the water is as follows: lactobacillus casei and lactobacillus plantarum: brown sugar: water 5: 2: 40;
b, oxygenating and activating the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the brown sugar and water for 2-3 hours to prepare an activated bacterium solution II for later use, wherein the weight ratio of the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the brown sugar to the water is as follows: bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae: brown sugar: water 5: 2: 40;
mixing the activated bacterium liquid I and the activated bacterium liquid II to obtain a mixture, adding water into the mixture to prepare a zymogen liquid, wherein the weight ratio of the activated bacterium liquid I to the activated bacterium liquid II is 3: 2, the weight ratio of the mixture to the water is 1: 80.
the weight ratio of the zymocyte liquid to the zymolyte is as follows: 300: 1000.
the preparation method of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda comprises the following steps:
step 1: uniformly mixing a fermentation substrate and a zymogen liquid to prepare a fermentation material;
step 2: stacking the fermented materials in a fermentation tank, wherein the height of the fermented materials is 60 cm;
and step 3: and (3) periodically turning over the fermented material by monitoring the change of the temperature and the pH value of the fermented material, and alternately performing aerobic fermentation and anaerobic fermentation, wherein the fermentation periods of the aerobic fermentation and the anaerobic fermentation are both 80h, so as to obtain the finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda.
The manner of monitoring the temperature and pH changes of the fermentation material includes: an electronic thermometer is arranged in the fermentation tank for temperature monitoring, the temperature is recorded once every two hours, and the rake is turned when the temperature is higher than 47 ℃, so that the aerobic fermentation and the anaerobic fermentation are alternately carried out;
simultaneously monitoring the pH value, wherein the method comprises the following steps: and (4) carrying out pH value detection on the fermented material every 18 hours, and obtaining a finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda when the pH value is less than 4.5.
In the embodiment, preferably, the feeding natural plants with health-care efficacy on the liver and intestine of the organism are compatible, the modern microbial fermentation technology is combined, the multi-strain aerobic and anaerobic deep fermentation mode is utilized to better release the active ingredients, and the efficacies of tonifying middle-jiao and Qi, strengthening intestine and helping digestion, nourishing blood and liver and soothing liver and depression are fully exerted; the product adopts high-quality nutritional feed raw materials such as corn, bean pulp, bran and the like and feedable natural plants with pharmacological action to prepare a fermentation substrate, can fully meet the growth and propagation advantages of microorganisms, generates rich nutritional metabolites and high-activity Chinese herbal medicine components after deep fermentation, replaces part or all baits in the liver-turning period and the enteritis outbreak period, and can better promote the ingestion of animals, enhance the physique and improve the resistance. Achieves the integration of medicine and material, and promotes the treatment and rehabilitation of diseases.
Example 2:
as shown in figure 1, the formula of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda comprises the following components:
fermentation substrate and zymogen liquid;
the fermentation substrate comprises corn, soybean meal, rhizoma atractylodis, ligusticum wallichii, mangnolia officinalis, Chinese yam, radix paeoniae alba, eucommia ulmoides and bran;
the corn, the bean pulp, the rhizoma atractylodis, the ligusticum wallichii, the mangnolia officinalis, the Chinese yam, the white paeony root and the eucommia ulmoides are crushed to 35 meshes and then mixed with the bran of 35 meshes to prepare the fermentation substrate.
The weight parts of the corn are 42 parts, the weight parts of the soybean meal are 22 parts, the weight parts of the bran are 17 parts, the weight parts of the rhizoma atractylodis are 12 parts, the weight parts of the ligusticum wallichii are 15 parts, the weight parts of the magnolia officinalis are 17 parts, the weight parts of the Chinese yam are 22 parts, the weight parts of the white paeony root are 17 parts, and the weight parts of the eucommia ulmoides are 15 parts.
The raw materials of the zymocyte liquid comprise: lactobacillus casei, lactobacillus plantarum, bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, brown sugar and water;
the strain ratio of the lactobacillus casei, the lactobacillus plantarum, the bacillus subtilis, the bacillus licheniformis and the saccharomyces cerevisiae is as follows: lactobacillus casei: lactobacillus plantarum: b, bacillus subtilis: b, bacillus licheniformis: 5, saccharomyces cerevisiae: 3: 5: 3: 5;
sealing and activating lactobacillus casei, lactobacillus plantarum, brown sugar and water for 4 hours to prepare activated bacterium liquid I for later use, wherein the weight ratio of the lactobacillus casei to the lactobacillus plantarum to the brown sugar to the water is as follows: lactobacillus casei and lactobacillus plantarum: brown sugar: water 10: 5: 80;
b, oxygenating and activating the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the brown sugar and water for 2.5 hours to prepare an activated bacterium solution II for later use, wherein the weight ratio of the bacillus subtilis to the bacillus licheniformis to the saccharomyces cerevisiae to the brown sugar to the water is as follows: bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae: brown sugar: water 15: 5: 80;
mixing the activated bacterium liquid I and the activated bacterium liquid II to obtain a mixture, adding water into the mixture to prepare a zymogen liquid, wherein the weight ratio of the activated bacterium liquid I to the activated bacterium liquid II is 6: 4, the weight ratio of the mixture to the water is 1: 90.
the weight ratio of the zymocyte liquid to the zymolyte is as follows: 350: 1000.
the preparation method of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda comprises the following steps:
step 1: uniformly mixing a fermentation substrate and a zymogen liquid to prepare a fermentation material;
step 2: stacking the fermented materials in a fermentation tank, wherein the height of the fermented materials is 70 cm;
and step 3: and (3) periodically turning over the fermented material by monitoring the change of the temperature and the pH value of the fermented material, and alternately performing aerobic fermentation and anaerobic fermentation, wherein the fermentation periods of the aerobic fermentation and the anaerobic fermentation are both 100 hours, so as to obtain the finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda.
The manner of monitoring the temperature and pH changes of the fermentation material includes: an electronic thermometer is arranged in the fermentation tank for temperature monitoring, the temperature is recorded once every two hours, and the rake is turned when the temperature is higher than 47 ℃, so that the aerobic fermentation and the anaerobic fermentation are alternately carried out;
simultaneously monitoring the pH value, wherein the method comprises the following steps: and (4) carrying out pH value detection on the fermented material every 18 hours, and obtaining a finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda when the pH value is less than 4.5.
In the embodiment, preferably, the feeding natural plants with health-care efficacy on the liver and intestine of the organism are compatible, the modern microbial fermentation technology is combined, the multi-strain aerobic and anaerobic deep fermentation mode is utilized to better release the active ingredients, and the efficacies of tonifying middle-jiao and Qi, strengthening intestine and helping digestion, nourishing blood and liver and soothing liver and depression are fully exerted; the product adopts high-quality nutritional feed raw materials such as corn, bean pulp, bran and the like and feedable natural plants with pharmacological action to prepare a fermentation substrate, can fully meet the growth and propagation advantages of microorganisms, generates rich nutritional metabolites and high-activity Chinese herbal medicine components after deep fermentation, replaces part or all baits in the liver-turning period and the enteritis outbreak period, and can better promote the ingestion of animals, enhance the physique and improve the resistance. Achieves the integration of medicine and material, and promotes the treatment and rehabilitation of diseases.
Example 3:
as shown in figure 1, the formula of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda comprises the following components:
fermentation substrate and zymogen liquid;
the fermentation substrate comprises corn, soybean meal, rhizoma atractylodis, ligusticum wallichii, mangnolia officinalis, Chinese yam, radix paeoniae alba, eucommia ulmoides and bran;
the corn, the bean pulp, the rhizoma atractylodis, the ligusticum wallichii, the mangnolia officinalis, the Chinese yam, the white paeony root and the eucommia ulmoides are crushed to 40 meshes and then mixed with the bran of 40 meshes to prepare the fermentation substrate.
The weight parts of the corn are 45 parts, the weight parts of the soybean meal are 25 parts, the weight parts of the bran are 20 parts, the weight parts of the rhizoma atractylodis are 15 parts, the weight parts of the ligusticum wallichii are 18 parts, the weight parts of the magnolia officinalis are 20 parts, the weight parts of the Chinese yam are 25 parts, the weight parts of the white paeony root are 20 parts, and the weight parts of the eucommia ulmoides are 8 parts.
The raw materials of the zymocyte liquid comprise: lactobacillus casei, lactobacillus plantarum, bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, brown sugar and water;
the strain ratio of the lactobacillus casei, the lactobacillus plantarum, the bacillus subtilis, the bacillus licheniformis and the saccharomyces cerevisiae is as follows: lactobacillus casei: lactobacillus plantarum: b, bacillus subtilis: b, bacillus licheniformis: 7, saccharomyces cerevisiae: 4: 7: 4: 6;
sealing and activating lactobacillus casei, lactobacillus plantarum, brown sugar and water for 5 hours to prepare an activated bacterium solution I for later use, wherein the weight ratio of the lactobacillus casei to the lactobacillus plantarum to the brown sugar to the water is as follows: lactobacillus casei and lactobacillus plantarum: brown sugar: water 15: 8: 120 of a solvent;
b, oxygenating and activating the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the brown sugar and water for 3 hours to prepare an activated bacterium solution II for later use, wherein the weight ratio of the bacillus subtilis to the bacillus licheniformis to the saccharomyces cerevisiae to the brown sugar to the water is as follows: bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae: brown sugar: 25 parts of water: 8: 120 of a solvent;
mixing the activated bacterium liquid I and the activated bacterium liquid II to obtain a mixture, adding water into the mixture to prepare a zymogen liquid, wherein the weight ratio of the activated bacterium liquid I to the activated bacterium liquid II is 9: 6, the weight ratio of the mixture to the water is 1: 100.
the weight ratio of the zymocyte liquid to the zymolyte is as follows: 400: 1000.
the preparation method of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda comprises the following steps:
step 1: uniformly mixing a fermentation substrate and a zymogen liquid to prepare a fermentation material;
step 2: stacking the fermented materials in a fermentation tank, wherein the height of the fermented materials is 80 cm;
and step 3: and (3) periodically turning over the fermented material by monitoring the change of the temperature and the pH value of the fermented material, and alternately performing aerobic fermentation and anaerobic fermentation, wherein the fermentation periods of the aerobic fermentation and the anaerobic fermentation are both 120h, so as to obtain the finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda.
In the embodiment, preferably, the feeding natural plants with health-care efficacy on the liver and intestine of the organism are compatible, the modern microbial fermentation technology is combined, the multi-strain aerobic and anaerobic deep fermentation mode is utilized to better release the active ingredients, and the efficacies of tonifying middle-jiao and Qi, strengthening intestine and helping digestion, nourishing blood and liver and soothing liver and depression are fully exerted; the product adopts high-quality nutritional feed raw materials such as corn, bean pulp, bran and the like and feedable natural plants with pharmacological action to prepare a fermentation substrate, can fully meet the growth and propagation advantages of microorganisms, generates rich nutritional metabolites and high-activity Chinese herbal medicine components after deep fermentation, replaces part or all baits in the liver-turning period and the enteritis outbreak period, and can better promote the ingestion of animals, enhance the physique and improve the resistance. Achieves the integration of medicine and material, and promotes the treatment and rehabilitation of diseases.
The manner of monitoring the temperature and pH changes of the fermentation material includes: an electronic thermometer is arranged in the fermentation tank for temperature monitoring, the temperature is recorded once every two hours, and the rake is turned when the temperature is higher than 47 ℃, so that the aerobic fermentation and the anaerobic fermentation are alternately carried out;
simultaneously monitoring the pH value, wherein the method comprises the following steps: and (4) carrying out pH value detection on the fermented material every 18 hours, and obtaining a finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda when the pH value is less than 4.5.
The product of the embodiment 1 is tested at 10 culture pond mouths, the area of each pond mouth is 40 mu, 5 control groups and 5 test groups, the control groups are fed with fresh ice feed, 20% of the product of the embodiment is added on the basis of the fresh ice feed of the test groups, 2 jin of the product of the embodiment is splashed for 1 time per mu in the whole pond every 7-10 days, the two groups of culture densities and conditions are consistent, and the culture period is 5-11 months, so that the test results shown in the table 1 are obtained.
TABLE 1
The tests show that the normal operation of various physiological functions of the intestinal tract is closely connected with the dynamic balance among intestinal flora, intestinal epithelial cells and an immune system, the intestinal tract is a main place for digesting and absorbing nutrient substances, the height of villus and the thickness of a muscular layer maintain main indexes of the intestinal absorption function, the increase of the height of villus and the thickness of the muscular layer can improve the digestion and absorption capacity of the intestinal tract to the nutrient substances, and intestinal beneficial bacteria have obvious effects on maintaining the steady state and the health of the intestinal tract, after the product of the embodiment 1 is applied, the height of villus of the caucasia carinatus intestine is 64.07 percent, the thickness of the muscular layer is 42 percent, the quantity of intestinal lactobacillus is increased by one order of magnitude, the bait coefficient is reduced by 13.56 percent, the intestinal tract health care effect is achieved, meanwhile, the activity of liver superoxide dismutase is improved by 45.07 percent, the total oxidation resistance of the liver is improved by 32.42 percent, the liver injury can be effectively relieved, in addition, the contents of harmful substances of ammonia nitrogen and nitrite in a water body and the quantity of pathogenic bacteria are reduced, the toxic action and the disease incidence of animals are reduced, and the results show that the incidence of the liver and intestine diseases is reduced by more than 56.52% by applying the product of the embodiment 1, the yield per mu is improved by more than 50%, and the product has obvious disease prevention and control effects and economic benefits.
The present invention has been described in an illustrative manner by the embodiments, and it should be understood by those skilled in the art that the present disclosure is not limited to the embodiments described above, but is capable of various changes, modifications and substitutions without departing from the scope of the present invention.
Claims (6)
1. The formula of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda is characterized by comprising the following components in parts by weight:
fermentation substrate and zymogen liquid;
the fermentation substrate comprises corn, soybean meal, rhizoma atractylodis, ligusticum wallichii, mangnolia officinalis, Chinese yam, radix paeoniae alba, eucommia ulmoides and bran;
the corn, the bean pulp, the rhizoma atractylodis, the ligusticum wallichii, the mangnolia officinalis, the Chinese yam, the white paeony root and the eucommia ulmoides are ground into 30-40 meshes, and then mixed with 30-40 meshes of bran to prepare the fermentation substrate.
2. The formula of the exopalaemon carinicauda liver and intestine health-care natural plant fermented feed as claimed in claim 1, wherein the corn is 40-45 parts by weight, the soybean meal is 20-25 parts by weight, the bran is 15-20 parts by weight, the rhizoma atractylodis is 10-15 parts by weight, the ligusticum wallichii is 12-18 parts by weight, the mangnolia officinalis is 15-20 parts by weight, the Chinese yam is 20-25 parts by weight, the radix paeoniae alba is 15-20 parts by weight, and the eucommia ulmoides is 13-18 parts by weight.
3. The formula of the natural plant fermented feed for the liver and intestine health care of the exopalaemon carinicauda according to claim 1, wherein the raw materials of the zymocyte liquid comprise: lactobacillus casei, lactobacillus plantarum, bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, brown sugar and water;
the strain ratio of the lactobacillus casei, the lactobacillus plantarum, the bacillus subtilis, the bacillus licheniformis and the saccharomyces cerevisiae is as follows: lactobacillus casei: lactobacillus plantarum: b, bacillus subtilis: b, bacillus licheniformis: saccharomyces cerevisiae ═ (3-7): (2-4): (3-7): (2-4): (4-6);
sealing and activating lactobacillus casei, lactobacillus plantarum, brown sugar and water for 3-5 hours to prepare activated bacterium liquid I for later use, wherein the weight ratio of the lactobacillus casei to the lactobacillus plantarum to the brown sugar to the water is as follows: lactobacillus casei and lactobacillus plantarum: brown sugar: water ═ 5-15: (2-8): (40-120);
b, oxygenating and activating the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the brown sugar and water for 2-3 hours to prepare an activated bacterium solution II for later use, wherein the weight ratio of the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the brown sugar to the water is as follows: bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae: brown sugar: water ═ 5-25: (2-8): (40-120);
mixing the activated bacterium liquid I and the activated bacterium liquid II to obtain a mixture, adding water into the mixture to prepare a zymogen liquid, wherein the weight ratio of the activated bacterium liquid I to the activated bacterium liquid II is (3-9): (2-6), wherein the weight ratio of the mixture to the water is 1: (80-100).
4. The formula of the natural plant fermented feed for the liver and intestine health care of the exopalaemon carinicauda according to claim 1, wherein the weight ratio of the zymocyte liquid to the fermentation substrate is as follows: (300-400): 1000.
5. a preparation method of a liver and intestine health-care natural plant fermented feed for exopalaemon carinicauda is characterized by comprising the following steps:
step 1: uniformly mixing a fermentation substrate and a zymogen liquid to prepare a fermentation material;
step 2: stacking the fermented materials in a fermentation tank, wherein the height of the fermented materials is 60-80 cm;
and step 3: the fermented materials are periodically turned over by monitoring the change of the temperature and the pH value of the fermented materials, so that aerobic fermentation and anaerobic fermentation are alternately performed, wherein the fermentation periods of the aerobic fermentation and the anaerobic fermentation are both 80-120 h, and the finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda is obtained.
6. The method for preparing the fermented feed of the natural plants for the health care of the liver and the intestine of the exopalaemon carinicauda according to claim 5, wherein the manner for monitoring the changes of the temperature and the pH value of the fermented material comprises the following steps: an electronic thermometer is arranged in the fermentation tank for temperature monitoring, the temperature is recorded once every two hours, and the rake is turned when the temperature is higher than 47 ℃, so that the aerobic fermentation and the anaerobic fermentation are alternately carried out;
simultaneously monitoring the pH value, wherein the method comprises the following steps: and (4) carrying out pH value detection on the fermented material every 18 hours, and obtaining a finished product of the liver and intestine health-care natural plant fermented feed for the exopalaemon carinicauda when the pH value is less than 4.5.
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| CN116616367A (en) * | 2023-02-17 | 2023-08-22 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of lactobacillus casei in preparation of preparation for enhancing cold resistance of fish |
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| CN116616367A (en) * | 2023-02-17 | 2023-08-22 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of lactobacillus casei in preparation of preparation for enhancing cold resistance of fish |
| CN116616367B (en) * | 2023-02-17 | 2024-05-17 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of lactobacillus casei in preparation of preparation for enhancing cold resistance of fish |
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