CN113881664A - Large-sample-quantity nucleic acid extraction method - Google Patents
Large-sample-quantity nucleic acid extraction method Download PDFInfo
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- CN113881664A CN113881664A CN202111347651.4A CN202111347651A CN113881664A CN 113881664 A CN113881664 A CN 113881664A CN 202111347651 A CN202111347651 A CN 202111347651A CN 113881664 A CN113881664 A CN 113881664A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention belongs to the field of nucleic acid extraction, and particularly relates to a method for extracting nucleic acid with a large sample amount, aiming at the problem of low extraction efficiency, the following scheme is proposed, which comprises the following steps: s1: combining 1, 2 and 3 columns of the kit into 1 column; s2: when in use, the cracking and the combination are carried out on the connected columns 1, 2 and 3; s3: washing in columns 4 and 5, elution in column 6, and so on in columns 7-12; s4: in the cracking and combining stage, when uniformly mixing, controlling an X-axis motor to enable the magnetic rod to vibrate left and right along the X axis; s5: in the washing and elution stages, a Z-axis motor is controlled to enable a magnetic rod to vibrate up and down along the Z-axis direction, the kit 1, 2 and 3 are communicated, the sample amount extracted once is 3 times of the original sample amount, when the sample amount required to be detected is more, the extraction efficiency can be increased by 3 times, the sample extraction amount can be increased, and the extraction efficiency and the sample types extracted once are improved.
Description
Technical Field
The invention relates to the technical field of nucleic acid extraction, in particular to a method for extracting nucleic acid with a large sample amount.
Background
The process of extracting nucleic acid by the magnetic bead method mainly comprises four steps of cracking, combining, washing and eluting, and nucleic acid extractors on the market mainly comprise two types of 32-flux and 96-flux, wherein 32-flux can extract 32 samples at a time, 96-flux can extract 96 samples at a time, and 32-flux is more favored by customers due to small volume and low price in terms of market acceptance. The kit used in the 32-flux nucleic acid extractor is a rectangular kit with 8 rows by 12 columns of holes, when in use, the lysis and the combination are carried out in the 1 st column, the combination is carried out in the 2, 3 and 4 columns, the 5 th column is used for removing magnetic beads, the 6 th column is used for elution, the 7-12 columns and the like, one hole of the kit can contain at most 200 and 250ul of samples, when the amount of the samples required for detection exceeds 250ul, the 2 holes are used for extracting the samples, and thus the extraction efficiency is reduced invisibly.
Disclosure of Invention
The invention aims to solve the defects of small sample extraction volume and low extraction efficiency of a single reagent hole in the prior art, and provides a method for extracting nucleic acid with large sample amount.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for extracting nucleic acid with multiple sample volumes comprises the following steps:
s1: combining 1, 2 and 3 columns of the kit into 1 column;
s2: when in use, the cracking and the combination are carried out on the connected columns 1, 2 and 3;
s3: washing in columns 4 and 5, elution in column 6, and so on in columns 7-12;
s4: in the cracking and combining stage, when uniformly mixing, controlling an X-axis motor to enable the magnetic rod to vibrate left and right along the X axis;
s5: and in the washing and elution stages, the Z-axis motor is controlled, so that the magnetic rod vibrates up and down along the Z-axis direction.
Preferably, in S3, cleavage and binding are performed in columns 7, 8 and 9.
Preferably, in S3, the washing is performed in columns 10 and 11, and the elution is performed in column 12.
Compared with the prior art, the invention has the beneficial effects that:
the kit 1, 2 and 3 are communicated in rows, so that the number of single samples can reach 600ul-750ul, the volume of the single extracted sample is 3 times that of the original sample, and when the volume of the sample required to be detected is larger, the extraction efficiency can be increased by 3 times;
the invention can increase the volume of the sample extracted at a single time, and improve the extraction efficiency and the sample type extracted at a single time.
Drawings
FIG. 1 is a schematic structural diagram of a nucleic acid extractor for a large sample amount nucleic acid extraction method according to the present invention;
FIG. 2 is a schematic diagram of a kit structure of a method for extracting nucleic acid with a large sample size according to the present invention;
FIG. 3 is a schematic diagram of the side view structure of FIG. 1 of the method for extracting nucleic acid with large sample size according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1 to 3, a method for extracting a large sample amount of nucleic acid includes the steps of:
s1: combining 1, 2 and 3 columns of the kit into 1 column;
s2: when in use, the cracking and the combination are carried out on the connected columns 1, 2 and 3;
s3: washing in columns 4 and 5, elution in column 6, and so on in columns 7-12;
s4: in the cracking and combining stage, when uniformly mixing, controlling an X-axis motor to enable the magnetic rod to vibrate left and right along the X axis;
s5: and in the washing and elution stages, the Z-axis motor is controlled, so that the magnetic rod vibrates up and down along the Z-axis direction.
The kit 1, 2 and 3 are communicated in series, so that the number of single samples can reach 600ul-750ul, the sample amount extracted in a single time is 3 times of the original sample amount, and the extraction efficiency can be increased by 3 times when the sample amount required to be detected is more.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (3)
1. A method for extracting nucleic acid with large sample amount is characterized by comprising the following steps:
s1: combining 1, 2 and 3 columns of the kit into 1 column;
s2: when in use, the cracking and the combination are carried out on the connected columns 1, 2 and 3;
s3: washing in columns 4 and 5, elution in column 6, and so on in columns 7-12;
s4: in the cracking and combining stage, when uniformly mixing, controlling an X-axis motor to enable the magnetic rod to vibrate left and right along the X axis;
s5: and in the washing and elution stages, the Z-axis motor is controlled, so that the magnetic rod vibrates up and down along the Z-axis direction.
2. The method for extracting large sample amount of nucleic acid according to claim 1, wherein in S3, cleavage and binding are performed at columns 7, 8 and 9.
3. The method according to claim 1, wherein in S3, washing is performed in columns 10 and 11, and elution is performed in column 12.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111347651.4A CN113881664A (en) | 2021-11-15 | 2021-11-15 | Large-sample-quantity nucleic acid extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111347651.4A CN113881664A (en) | 2021-11-15 | 2021-11-15 | Large-sample-quantity nucleic acid extraction method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113881664A true CN113881664A (en) | 2022-01-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111347651.4A Withdrawn CN113881664A (en) | 2021-11-15 | 2021-11-15 | Large-sample-quantity nucleic acid extraction method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113881664A (en) |
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2021
- 2021-11-15 CN CN202111347651.4A patent/CN113881664A/en not_active Withdrawn
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| PB01 | Publication | ||
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20220104 |
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| WW01 | Invention patent application withdrawn after publication |