CN113866432A

CN113866432A - Sample detection equipment and method thereof

Info

Publication number: CN113866432A
Application number: CN202010621698.4A
Authority: CN
Inventors: 谢子贤; 刘海
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2020-06-30
Filing date: 2020-06-30
Publication date: 2021-12-31

Abstract

A sample detection device and a method thereof, concretely, after controlling and detecting mixed sample liquid in a reaction tank each time, controlling and executing a conventional cleaning mode to clean the reaction tank, so that the reaction tank can detect the specific protein content of the next sample; when a preset temporary cleaning condition is reached, controlling to execute a temporary cleaning mode to clean the reaction tank; and when a preset regular cleaning time is reached, controlling to execute a deep cleaning mode to clean the reaction tank. The present application introduces three cleaning modes: a normal washing mode, a temporary washing mode, and a deep washing mode, which can maintain the reaction cell in a washing state for a long time.

Description

Sample detection equipment and method thereof

Technical Field

The invention relates to a sample detection device and a method thereof.

Background

With the popularization of clinical applications, more and more parameters need to be detected in the field of blood tests. From the first three and five categories of blood routine to the later blood routine to be tested simultaneously with the detection of specific proteins. At present, the detection of specific proteins is being promoted, in addition to blood routine + CRP (C-Reactive Protein), also blood routine + SAA, blood routine + CRP + SAA or even more parameters (e.g. PCT, IL6, etc.). In order to achieve the ease of detection, it is generally necessary to perform the above-mentioned parameter detection with one blood sample, that is, to implement a blood routine and various protein-specific integration machines.

Detection of specific proteins is typically carried out using transmission and/or nephelometry. To detect the concentration of a specific protein (antigen) in blood, it is necessary to add specific latex particles (antibody) to the sample. The latex particles are nano-scale spherical particles, and can be combined with surrounding specific proteins under certain conditions to form micelles with larger volume. When the latex particles are continuously combined with the specific protein, the formed micelle assembly is enlarged, the scattered signal assembly formed after the latex particles are irradiated by the light with the specific wavelength is enhanced, and the transmitted signal is gradually weakened. The content of the specific protein in the sample can be obtained by monitoring the change rate of the transmission and/or scattering signal and certain calculation.

According to the above description, the essence of the reaction of the specific protein detection is the process of generating larger micelles by continuously combining the specific protein antigen and the antibody latex microspheres. These protein micelles easily adhere to the wall of the reaction cell during the formation process. After the test is finished, the cell wall needs to be cleaned, and the cell wall is restored to a measurement ready state, so that the detection result of the next sample is prevented from being influenced.

Therefore, the cleaning of the reaction chamber for detecting the specific protein is an essential factor for ensuring the accurate detection result of the specific protein.

Disclosure of Invention

The present application provides a sample testing device and method, as described in detail below.

According to a first aspect, there is provided in an embodiment a sample detection apparatus comprising:

the sample mechanism is used for providing a sample to be tested;

a reagent mechanism for providing a reagent for detecting a specific protein and a reagent for routine blood detection, the reagent for routine blood detection including one or more hemolytic agents;

a blood routine component for processing the sample to be tested provided by the sample mechanism by the blood routine detection reagent provided by the reagent mechanism to classify and/or count the cells of the sample to be tested;

a reaction cell for receiving the sample to be detected provided by the sample mechanism and the reagent for detecting the specific protein provided by the reagent mechanism so that the sample to be detected and the reagent react with each other to form a mixed sample solution;

a specific protein detection member including a detection region made of a light transmitting material, a light source and a receiver provided corresponding to the detection region, the light source being configured to irradiate the mixed sample liquid flowing through the detection region so that the receiver senses an optical signal related to a specific protein content in the mixed sample liquid, the optical signal being configured to detect the specific protein content in the mixed sample liquid;

the cleaning assembly is at least used for cleaning the reaction tank; the cleaning assembly has three cleaning modes: a regular washing mode, a temporary washing mode, and a deep washing mode, and wherein at least one washing mode uses the one or more hemolytic agents as a cleaning liquid to wash the reaction cell;

the controller for control washs the subassembly and washs the reaction tank specifically includes:

after the mixed sample liquid in the reaction tank is detected each time, the controller controls the cleaning assembly to execute a conventional cleaning mode to clean the reaction tank, so that the reaction tank can detect the specific protein content of the next sample;

when a preset temporary cleaning condition is reached, the controller controls the cleaning assembly to execute a temporary cleaning mode to clean the reaction tank;

and when the preset regular cleaning time is reached, the controller controls the cleaning assembly to execute a deep cleaning mode to clean the reaction tank.

In one embodiment, the reaction tank is provided with a waste liquid discharge port and a first cleaning port;

the sample detection device further comprises a waste liquid channel and a waste liquid driving part; one end of the waste liquid channel is connected with a waste liquid discharge port of the reaction tank, and the other end of the waste liquid channel is connected with the waste liquid driving part; the waste liquid driving part is used for discharging liquid in the reaction tank through the waste liquid channel;

the cleaning assembly further comprises a cleaning liquid supply part and a cleaning liquid supply part; the cleaning solution supply part comprises a cleaning solution supply channel connected with a first cleaning port of the reaction pool, and is used for supplying cleaning solution to the reaction pool through the cleaning solution supply channel; the cleaning liquid supply component is used for supplying the cleaning liquid to the reaction cell;

wherein the first cleaning port is eccentrically disposed such that the cleaning solution supplied from the cleaning solution supply part enters the reaction chamber from the first cleaning port in an eccentric direction.

In one embodiment, the first cleaning port is arranged along a tangential direction of the wall of the reaction tank.

In one embodiment, the cleaning liquid supply component further includes a recovery channel, one end of which is connected to the waste liquid discharge port, and the other end of which is connected to the first cleaning port, for recovering the cleaning liquid discharged from the waste liquid discharge port as the cleaning liquid for flushing the reaction tank.

In one embodiment, the reaction tank is provided with a second cleaning port; the cleaning liquid supply member includes a cleaning liquid supply passage connected to the second cleaning port of the reaction cell, the cleaning liquid supply member being for supplying the cleaning liquid to the reaction cell through the cleaning liquid supply passage;

wherein the second cleaning port is eccentrically disposed such that the cleaning liquid supplied from the cleaning liquid supply part enters the reaction cell from the second cleaning port in an eccentric direction.

In one embodiment, the second cleaning port is arranged along a tangential direction of the wall of the reaction tank.

In one embodiment, the cleaning liquid supply unit is used for reusing the reagent mechanism to supply one or more hemolytic agents as the cleaning liquid to the reaction cell during cleaning.

In one embodiment, the time consumption of one regular cleaning mode is less than that of one temporary cleaning mode, and the time consumption of one temporary cleaning mode is less than or equal to that of one deep cleaning mode.

According to a second aspect, an embodiment provides a method of sample detection apparatus, comprising:

controlling to suck a sample to be detected, and respectively discharging a sample to be detected into a conventional blood reaction pool and a reaction pool for detecting specific protein;

controlling the addition of one or more hemolytic agents to the blood conventional reaction tank;

controlling the test of at least one item in the blood routine of the sample to be tested treated by the hemolytic agent;

controlling the addition of a latex reagent for reaction to a reaction cell for specific protein detection to prepare a mixed sample solution;

controlling irradiation on the mixed sample liquid to detect the content of specific protein in the mixed sample liquid;

controlling the cleaning of the reaction cell, wherein the method comprises three cleaning modes of the reaction cell: a regular washing mode, a temporary washing mode, and a deep washing mode, and wherein at least one washing mode uses the one or more hemolytic agents as a cleaning liquid to wash the reaction cell; the control performs washing of the reaction cell, including:

after the mixed sample liquid in the reaction tank is controlled and detected each time, a conventional cleaning mode is controlled to be executed to clean the reaction tank, so that the reaction tank can detect the specific protein content of the next sample;

when a preset temporary cleaning condition is reached, controlling to execute a temporary cleaning mode to clean the reaction tank;

and when a preset regular cleaning time is reached, controlling to execute a deep cleaning mode to clean the reaction tank.

According to a third aspect, an embodiment provides a method of a sample detection apparatus, comprising:

controlling to suck a sample to be detected, and discharging the sample to be detected into a reaction tank for detecting the specific protein;

controlling the cleaning of the reaction cell, wherein the method comprises three cleaning modes of the reaction cell: a normal cleaning mode, a temporary cleaning mode and a deep cleaning mode; the control performs washing of the reaction cell, including:

In one embodiment, the normal cleaning mode includes:

and controlling to use a cleaning solution to flush the reaction tank.

In one embodiment, the controlling the washing of the reaction tank with the washing liquid includes:

and controlling to add the cleaning liquid into the reaction tank in an eccentric direction, wherein the eccentric direction is the tangential direction of the wall of the reaction tank.

In one embodiment, the normal cleaning mode further comprises:

while controlling the washing of the reaction tank by using the washing liquid, continuously discharging the washing liquid in the reaction tank as waste liquid, or,

and continuously discharging the cleaning liquid in the reaction tank and recovering the cleaning liquid as the cleaning liquid for cleaning the reaction tank while controlling the cleaning liquid to be used for cleaning the reaction tank.

In one embodiment, the normal cleaning mode further comprises:

and controlling to add cleaning solution into the reaction tank, and discharging the cleaning solution as waste liquid after soaking the reaction tank for a preset time.

In one embodiment, the cleaning solution comprises one or more hemolysing agents.

In one embodiment, the deep cleaning mode comprises:

and controlling one or more cleaning liquids to be added into the reaction tank, and discharging the reaction tank as waste liquid after soaking for a preset time.

In one embodiment, the deep cleaning mode further comprises:

and controlling to flush the reaction tank by using a cleaning solution or the cleaning solution.

In one embodiment, the deep cleaning mode further comprises:

while controlling the washing of the reaction tank with the washing liquid/the cleaning liquid, continuously discharging the washing liquid/the cleaning liquid in the reaction tank as waste liquid, or,

and continuously discharging the cleaning liquid/the cleaning liquid in the reaction tank and recovering the discharged cleaning liquid/the cleaning liquid as the cleaning liquid/the cleaning liquid for flushing the reaction tank while controlling the flushing of the reaction tank by using the cleaning liquid/the cleaning liquid.

In one embodiment, the preset periodic cleaning timing comprises any one or more of the following:

when the sample detection equipment is started up every day;

when the sample detection equipment is shut down every day;

when the sample detection equipment enters dormancy;

when the sample detection device exits from sleep;

a preset periodic cleaning time point;

a deep cleaning start command is received.

In one embodiment, the temporary cleaning mode includes:

controlling to add one or more cleaning liquids into the reaction tank, and discharging the liquid as waste liquid after soaking the reaction tank for a preset time; and/or the presence of a gas in the gas,

and controlling to flush the reaction tank by using a cleaning solution or a cleaning solution.

In one embodiment, the preset temporary cleaning conditions include any one or more of the following:

judging whether the accumulated test times of the detection of the content of the specific protein in the reaction tank reaches a set time after the deep cleaning mode or the temporary cleaning mode is executed on the reaction tank for the last time, and if so, controlling to execute the temporary cleaning mode to clean the reaction tank;

judging whether the accumulated times of the specified measurement mode in the reaction tank reaches a set time after the deep cleaning mode or the temporary cleaning mode is executed on the reaction tank for the last time, and if so, controlling to execute the temporary cleaning mode to clean the reaction tank;

judging whether the accumulated value of the detection result of the content of the specific protein in the reaction tank reaches a set value after the deep cleaning mode or the temporary cleaning mode is executed on the reaction tank for the last time, and controlling to execute the temporary cleaning mode to clean the reaction tank if the accumulated value reaches the set value;

judging whether the number of samples with detection results larger than a set value in samples for detecting the content of the specific protein in the reaction tank reaches a set number after the reaction tank is subjected to a deep cleaning mode or a temporary cleaning mode for the last time, and if so, controlling to execute the temporary cleaning mode to clean the reaction tank;

acquiring blank voltage of a reaction tank, and controlling to execute a temporary cleaning mode to clean the reaction tank when the blank voltage is within a preset range; the blank voltage is: when the reaction cell is filled with specific liquid, the specific liquid in the reaction cell is irradiated by control, and transmitted or scattered light is induced and converted into voltage. For example, the preset range of the blank voltage acquired by the scattering principle is greater than a set voltage, and the preset range of the blank voltage acquired by the transmission principle is less than a set voltage.

In one embodiment, the specific liquid is a cleaning liquid or a cleaning liquid; and when the reaction tank is controlled to execute a conventional cleaning mode to clean the reaction tank, the blank voltage is controlled to be collected.

According to a fourth aspect, an embodiment provides a computer readable storage medium comprising a program executable by a processor to implement the method of any of the embodiments herein.

According to an eleventh aspect, an embodiment provides a computer readable storage medium comprising a program executable by a processor to implement a method as described in any of the embodiments herein

According to the sample testing apparatus, the method thereof and the computer-readable storage medium of the above embodiments, three washing modes are introduced: a normal washing mode, a temporary washing mode, and a deep washing mode, which can maintain the reaction cell in a washing state for a long time.

Drawings

FIG. 1 is a schematic structural diagram of a sample testing device according to an embodiment;

FIG. 2 is a schematic diagram of the structure of a specific protein detection unit according to an embodiment;

FIG. 3 is a schematic diagram of an exemplary cleaning assembly;

FIG. 4 is a schematic view of an embodiment in which the first cleaning port is eccentrically disposed;

FIG. 5 is a schematic view of an alternate embodiment in which the first purge port is eccentrically positioned;

FIG. 6 is a schematic diagram of an embodiment of a cleaning solution supply assembly further comprising a recovery channel;

FIG. 7 is a schematic structural view of another embodiment of a cleaning assembly;

FIG. 8 is a schematic structural view of a cleaning assembly according to yet another embodiment;

FIG. 9 is a flow diagram of a method of a sample detection device of an embodiment;

FIG. 10 is a flow chart of a method of a sample testing device of another embodiment.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

However, researches show that the protein generated in the specific protein detection process has strong adhesive capacity, and common washing is used, so that no way is provided for cleaning all the proteins on the wall of the pool; and in addition, under the condition of long-term test accumulation, more and more proteins are adhered to the tank wall, so that the light transmittance of the tank body is continuously reduced, and the normal test is influenced. In addition, protein adhesion has certain influence on the carrying pollution of normal measurement, so that the measurement result is deviated.

In view of the above situation, the present application introduces three cleaning modes, i.e., a normal cleaning mode, a temporary cleaning mode, and a deep cleaning mode, so that the reaction cell can be protected in a good state for a long time, as described in detail below.

Some embodiments provide a sample detection device that can perform routine blood tests and specific protein tests, for example, the sample detection device can detect routine blood items and specific protein content from the same blood sample. Referring to fig. 1, the sample testing apparatus in some embodiments includes a sample mechanism 10, a reagent mechanism 20, a blood routine component 30, a reaction cell 40, a specific protein detection component 50, a washing component 60, and a controller 70, which are described in detail below.

The sample mechanism 10 is used to provide a sample to be tested. There are a variety of implementations of the sample mechanism 10. In some examples, the Sample mechanism 10 may include a Sample Dispensing Module (SDM), a front end rail, and a Sample needle capable of being driven to perform two-dimensional or three-dimensional movement, the Sample dispensing Module and the front end rail cooperating to supply a Sample tube containing a Sample and to transport the Sample tube containing the Sample to a location such as a Sample aspirating location, the Sample needle being capable of aspirating the Sample from the Sample tube at the Sample aspirating location and discharging the Sample to a corresponding location, such as the reaction cell 40 described above, and the like. In other examples, the sample mechanism 10 may also include a sample tray and a sample needle capable of being driven to perform two-dimensional or three-dimensional movement, the sample tray includes a plurality of sample sites on which sample tubes such as sample tubes can be placed, the sample tray can dispatch the samples to corresponding positions, such as a suction site, by rotating the tray structure thereof, and the sample needle can suck the samples from the sample tubes located at the suction site and discharge the samples to the corresponding positions, such as the reaction cell 40 described above.

The reagent mechanism 20 is used to supply a reagent for detection. In some examples, the reagent mechanism 20 may be a reagent disk equipped with a reagent needle, the reagent disk being provided in a disk-shaped structure having a plurality of positions for carrying reagent containers, the reagent disk being capable of rotating and carrying the reagent containers carried by the reagent disk for rotating the reagent containers to specific positions, such as positions for sucking reagent by the reagent needle. The reagent mechanism 20 is used to provide reagents for the detection of specific proteins and to provide reagents for the routine detection of blood, wherein the reagents for the routine detection of blood include one or more hemolytic agents.

The blood routine component 30 is used for processing the sample to be tested provided by the sample mechanism by the blood routine testing reagent provided by the reagent mechanism 20 to classify and/or count the cells of the sample to be tested. For example, the blood routine component 30 may include at least one detection module of a WBC (white blood cell) classification module, a WBC/HGB module, and a RBC/PLT module. The WBC classification module is used for obtaining a five-classification result of WBCs of a blood sample to be measured, the WBC/HGB module is used for finishing WBC counting and morphological parameter measurement and has a function of measuring HGB (hemoglobin), and the RBC/PLT module is used for finishing RBC (red blood cell), PLT (platelet) counting and morphological parameter measurement.

It can be seen that testing of a blood routine program on a blood sample can be accomplished by the cooperation of the sample mechanism 10, reagent mechanism 20 and blood routine component 30.

The reaction cell 40 is used for receiving the sample to be tested provided by the sample mechanism 10 and the reagent for detecting the specific protein, such as latex reagent, provided by the reagent mechanism 20, so that the two react to form a mixed sample solution. It is understood that, when the blood sample to be tested is serum or plasma containing no blood cells, the above-mentioned mixed sample solution can be prepared by reacting a reagent for detecting a specific protein, such as a latex reagent, with such a blood sample for detecting a specific protein; when the blood sample to be tested is a whole blood sample containing blood cells, it is necessary to add a hemolytic agent to the whole blood sample to dissolve the blood cells and then add a specific protein detection reagent such as a latex reagent to prepare the above-mentioned mixed sample solution to perform detection of the specific protein. Of course, it will be understood by those skilled in the art that in some instances, it may be desirable to also add reagents such as buffers and/or diluents.

Referring to fig. 2, the specific protein detecting component 50 includes a detecting area 51 made of a light-transmitting material, a light source 52 and a receiver 53, the light source 52 is configured to irradiate the mixed sample solution flowing through the detecting area 51, so that the receiver 53 senses an optical signal related to the content of the specific protein in the mixed sample solution, the optical signal is configured to detect the content of the specific protein in the mixed sample solution, the receiver 53 is configured to convert the optical signal into a corresponding electrical signal, and the electrical signal is processed and analyzed to obtain the content of the specific protein in the mixed sample solution.

The cleaning assembly 60 is used at least for cleaning the reaction cell 40. In some examples, the washing component 60 can also wash the sample mechanism 10, the reagent mechanism 20, the blood routine component 30, and the like, and the washing component 60 mainly focuses on how the reaction cell 40 is washed by the washing component 60.

In some embodiments, the cleaning assembly 60 has three cleaning modes: a normal cleaning mode, a temporary cleaning mode, and a deep cleaning mode, which are explained below.

The normal washing mode is a normal washing of the reaction cell 40 after completion of each sample test. In other words, each time the mixed sample solution in the reaction cell 40 is detected, the washing module 60 performs a normal washing mode to wash the reaction cell 40 and restore the reaction cell 40 to a measurement ready state, so as to avoid affecting the detection result of the next sample, thereby enabling the reaction cell 40 to perform the detection of the specific protein content of the next sample. The normal cleaning mode may be that the cleaning assembly 60 washes the reaction cell with a cleaning solution, which may be a diluent or the like. Of course, the conventional cleaning mode may also be combined with a cleaning solution, for example, a cleaning solution may be added to the reaction cell 40 to perform immersion cleaning on the reaction cell 40, and the cleaning solution may be a hemolytic agent. Generally, although most of the contaminants can be cleaned by the conventional cleaning mode, few contaminants may remain to adhere to the wall of the reaction cell 40, and thus there is an accumulative effect after a long time use, so that the contaminants in the reaction cell 40 can affect the accuracy of the detection result enough, and even the reaction cell 40 is irreversibly damaged, for example, the contaminants adhered to the wall of the reaction cell 40 cannot be removed by soaking in cleaning solution for a long time or manually scraping. In consideration of this situation, the present application is also provided with two cleaning modes, a temporary cleaning mode and a deep cleaning mode.

The deep cleaning mode is generally to perform a relatively deep cleaning on the reaction cell 40 at some special timing, such as a timing of turning on and off the device, before the test of the same day starts, or after the test of the same day ends. The deep cleaning mode may be that the cleaning assembly 60 uses one or more cleaning liquids to wash and/or soak the reaction cell 40 for a relatively long time, for example, at least 10 minutes or more, such as 30 minutes, so as to initialize the state of the reaction cell 40 and clean it thoroughly. The cleaning liquid involved in the deep wash mode may be a hemolytic agent. The deep cleaning mode may be timed to occur once or twice daily.

The temporary washing mode is typically performed during the current day of the test, and the sample testing apparatus is triggered by the preset temporary washing condition to allow the washing unit 60 to perform an additional washing operation on the reaction cell 40 based on the regular washing mode. The temporary cleaning mode may be similar to the normal cleaning mode or the deep cleaning mode, but since the temporary cleaning mode is a cleaning additionally added in the test process, the temporary cleaning mode may reduce the time in order to avoid the influence on the normal test, for example, the temporary cleaning mode may be similar to the deep cleaning mode, but compared to the deep cleaning mode, the temporary cleaning mode may reduce the cleaning time, reduce the suction and discharge times in the cleaning process, reduce the types of cleaning liquids used, and the like, taking the cleaning time as an example, the deep cleaning mode may be 10 minutes, and the temporary cleaning mode may be about 1 minute.

The above are some descriptions of the three cleaning modes, and various operations involving the cleaning assembly 60 using a cleaning solution, such as a cleaning solution of a hemolytic agent, soaking, and waste liquid during the cleaning process will be described below with reference to the related structures.

In some embodiments, referring to fig. 3, the reaction chamber 40 is provided with a waste liquid discharge port 40a and a first cleaning port 40 b. The waste liquid discharge port 40a is used for discharging a waste liquid such as a mixed sample liquid after detection, for example, a waste liquid generated by washing or the like. Specifically, the waste liquid channel 41a and the waste liquid driving part 41 may be introduced; one end of the waste liquid channel 41a is connected to the waste liquid discharge port 40a of the reaction tank 40, and the other end of the waste liquid channel 41a is connected to the waste liquid driving member 41; the waste liquid driving part 41 is used to discharge the liquid in the reaction cell 40 through the waste liquid channel 41a, for example, the waste liquid driving part 41 may include a pressure source to provide a negative pressure, so that the waste liquid in the reaction cell 40 can flow into the waste liquid channel 41a through the waste liquid discharge port 40a and be discharged. In some examples, the waste discharge port 40a or the waste passage 41a can be controllably opened and closed, for example, by a solenoid valve. In some examples, the waste outlet port 40a is provided at the bottom of the reaction tank 40.

The washing unit 60 can wash the reaction cell with a washing liquid. In some embodiments, the cleaning assembly 60 includes a cleaning solution supply member 61. The cleaning solution supply part 61 includes a cleaning solution supply passage 61a connected to the first cleaning port 40b of the reaction chamber 40, and the cleaning solution supply part 61 is used to supply a cleaning solution to the reaction chamber 40 through the cleaning solution supply passage 61 a. In some examples, the first cleaning port 40b is provided on a side wall of the reaction chamber 40. In a specific washing process, the washing liquid supply part 61 feeds the washing liquid into the reaction tank 40 through the first washing port 40b by, for example, positive pressure power, washes the tank wall or the like, and discharges the waste liquid through the waste liquid discharge port 40 a. The flushing action can be repeated for a plurality of times, and a better flushing effect is achieved.

In some embodiments, the first cleaning port 40b is eccentrically arranged, so that the cleaning liquid supplied by the cleaning liquid supply part 61 enters the reaction tank 40 from the first cleaning port 40b along an eccentric direction, and thus a swirling state is formed when the cleaning liquid is added into the reaction tank 40. For example, FIG. 4 is an example of a schematic view of the top view of the reaction cell 40, in which the major diameter direction of the first cleaning port 40b is eccentric, i.e., not aligned with the center of the cross section of the reaction cell. In some embodiments, the first cleaning port 40b is disposed along the tangential direction of the wall of the reaction tank, i.e., the wall of the reaction tank 40, so that the swirling state is better. Such is the case, for example, in fig. 5. Under the scheme of the rotational flow, the higher the flow (speed) of the cleaning liquid, the better the cleaning effect. Further, the inventor researches and invents that the cleaning is carried out by a rotational flow cleaning scheme, and the longer the rotational flow duration is, the better the cleaning effect is. However, after the cleaning liquid is added to the reaction tank 40, the rotational flow is gradually slowed down due to the dissipation of the capacity. If the liquid is continuously added at this time so that the swirling flow is continued, the liquid may overflow due to the limited volume of the tank body. The enhanced cleaning can be performed in the following two ways. First, while the cleaning liquid is added, the evacuation action is added so that the continuous swirling flow can be generated in the tank without causing the overflow — it is understood that the evacuation action herein refers to the action of discharging the waste liquid through the waste liquid discharge port 40 a. Alternatively, positive and negative pressure switching means may be incorporated in the cleaning liquid supply part 61, that is, the cleaning liquid supply part 61 may supply either positive or negative pressure; cleaning liquid is added into the reaction tank 40 through the cleaning liquid supply passage 61a via the first cleaning port 40b to form a rotational flow for cleaning, when the rotational flow is decelerated, liquid in the tank is sucked back into the cleaning liquid supply passage 61a through negative pressure, and then the liquid is added into the reaction tank 40 again through, for example, the first cleaning port 40b through positive pressure to form the rotational flow again; the suction and discharge mode can form continuous rotational flow characteristics in the pool to enhance cleaning; the suction and discharge times can be one or more; therefore, in some embodiments, referring to fig. 6, the cleaning solution supplying part 61 further includes a recycling channel 61b and a positive and negative pressure device 62, one end of the recycling channel 61b is connected to the waste liquid discharging port 40a, and the other end is connected to the positive and negative pressure device 62, and when the positive and negative pressure device 62 operates in a negative pressure state, waste liquid is sucked from the waste liquid discharging port 40a to the recycling channel 61 b. The positive and negative pressure device 62 is converted to positive pressure again to push the waste liquid in the recovery channel 61b back to the reaction tank 40, so as to recover the discharged cleaning liquid as the cleaning liquid for flushing the reaction tank 40.

The above describes how the cleaning assembly 60 supplies the cleaning solution and cleans the reaction cell 40 by the cleaning solution, and the following describes how the cleaning assembly 60 supplies the cleaning solution and cleans and/or soaks the reaction cell 40 by the cleaning solution.

The cleaning assembly 60 is capable of rinsing and/or soaking the reaction cell with a cleaning solution. The cleaning solution may be a hemolytic agent. In some embodiments, referring to fig. 7, the cleaning assembly 60 includes a cleaning liquid supply assembly 65. Specifically, the reaction tank 40 may be provided with a second purge port 40 c; the cleaning liquid supply part 65 includes a cleaning liquid supply passage 65a connected to the second washing port 40c of the reaction cell 40, and the cleaning liquid supply part 65 serves to supply the cleaning liquid to the reaction cell 40 through the cleaning liquid supply passage 65 a. The second purge port 40c may be disposed similarly to the first purge port 40b, for example, eccentrically, and further, for example, along the tangential direction of the wall of the reaction tank, i.e., the wall of the reaction tank 40, which will not be described herein again. The first and

second purge ports

40b, 40b may be the same port or two different ports. Fig. 7 shows an example in which the first purge port 40b and the second purge port 40b are two different ports, and fig. 8 shows an example in which the first purge port 40b and the second purge port 40b are the same port. In the case where the cleaning liquid is supplied from the cleaning liquid supply part 65 to wash the reaction well 40, the action may be similar to that of the cleaning liquid supply part 61 to wash the reaction well 40 with the cleaning liquid, for example, the drain action may be added simultaneously with the addition of the cleaning liquid, so that the continuous swirling flow can be generated in the well without causing the overflow; for another example, positive or negative pressure switching means may be incorporated in the cleaning liquid supply part 65, i.e., the cleaning liquid supply part 61 may supply positive or negative pressure; cleaning liquid is added into the reaction tank 40 through the cleaning liquid supply channel 65a via the second cleaning port 40c to form a rotational flow for cleaning, when the rotational flow is decelerated, the liquid in the tank is sucked back into the cleaning liquid supply channel 65a via negative pressure, and then the liquid is added into the reaction tank 40 again via the second cleaning port 40c via positive pressure, for example, to form the rotational flow again; the suction and discharge mode can form continuous rotational flow characteristics in the pool to enhance cleaning; the suction and discharge times can be one or more.

The above are some examples of the cleaning liquid supplied through the liquid path structure connected to the reaction cell 40. As mentioned above, the cleaning solution may be a hemolytic agent, and the routine blood detection in this application requires the use of a hemolytic agent to treat the blood sample, so the reagent mechanism can also provide a hemolytic agent. Therefore, in some components, the cleaning solution supply component 65 reuses the reagent mechanism 20 for supplying one or more hemolytic agents as the cleaning solution to the reaction cell 40 during cleaning.

The above describes how the cleaning assembly 60 cleans the reaction cell 40 with the cleaning solution and the cleaning solution, and the following further describes how the cleaning solution and the cleaning solution are used in various cleaning modes in combination with the three cleaning modes.

Normal cleaning mode

The controller 70 controls the washing unit 60 to perform a normal washing mode to wash the reaction cell 40 each time the mixed sample solution in the reaction cell is detected, so that the reaction cell 40 can perform the detection of the specific protein content of the next sample. Specifically, the detected mixed sample liquid may be drained through the waste liquid channel 41a, or during the draining of the mixed sample liquid, the cleaning liquid supply part 61 adds a cleaning liquid to the reaction chamber 40 through the first cleaning port 40b to flush the reaction chamber 40, and drains the waste liquid through the waste liquid draining port 40 a; the flushing action can be repeated for a plurality of times, and a better flushing effect is achieved. In the case where the first cleaning port 40b is eccentrically disposed, the cleaning liquid supplied from the cleaning liquid supply part 61 to the reaction tank 40 can be formed in a swirling state, and the evacuation action is added simultaneously with the supply of the cleaning liquid, so that a continuous swirling flow can be generated in the tank without causing an overflow. In the case of the recovery passage 61b, the cleaning liquid is introduced into the reaction tank 40 through the first cleaning port 40b via the cleaning liquid supply passage 61a to form a rotational flow for cleaning, and after the rotational flow is decelerated, the liquid in the tank is sucked back into the cleaning liquid supply passage 61a from the recovery passage 61b by negative pressure, and then the liquid is introduced into the reaction tank 40 again through, for example, the first cleaning port 40b by positive pressure to form a rotational flow again, and the suction and discharge times may be one or more times.

On the premise of rinsing with the cleaning liquid, the cleaning liquid supply part 65 may add the cleaning liquid to the reaction tank 40 to perform soaking for a preset time, for example, 5 seconds; the cleaning liquid reacts with the pollutants adhered to the pool wall to decompose and fall off, so that the effect of enhancing cleaning is achieved.

The cleaning solution rinsing and the cleaning solution soaking can be alternately performed one or more times, for example, the cleaning solution rinsing is performed once, the cleaning solution soaking is performed once again, and the cleaning solution rinsing is performed once again; then, for example, a cleaning solution soaking is performed first, and then a cleaning solution rinsing is performed. Typically, the last cleaning is a cleaning fluid rinse.

(II) deep cleaning mode

The controller 70 controls the cleaning assembly 60 to perform the deep cleaning mode to clean the reaction cell 40 when a preset periodic cleaning timing is reached. The preset periodic cleaning opportunities include any one or more of: the method comprises the following steps of starting up the sample detection device every day, stopping up the sample detection device every day, enabling the sample detection device to enter the sleep state, enabling the sample detection device to exit the sleep state, and setting a regular cleaning time point or receiving a deep cleaning starting command.

It can be seen that the normal cleaning mode is set in the test flow process and is a part of the test flow, while the deep cleaning mode generally interrupts or interrupts the test flow, or the deep cleaning mode is performed before the test flow has not started or after the test flow has ended. Since the conventional cleaning mode is set in the testing process, and the sample testing apparatus has certain requirements for speed and reagent consumption, the conventional cleaning mode is limited, such as requiring less time, which may result in incomplete cleaning, as mentioned above, so that a few contaminants still remain and adhere to the reaction cell 40. The deep washing mode is a washing mode which is more powerful than the conventional washing mode, and the deep washing mode is preferably arranged at a non-busy time, for example, at the time when the above-mentioned sample detection apparatus is turned on every day, when the sample detection apparatus is turned off every day, when the sample detection apparatus enters the sleep state, or when the sample detection apparatus exits the sleep state, or the like, a relatively thorough washing is performed by means of a powerful cleaning solution and a suitable increase in the washing time, so that the reaction cell 40 can be restored to the initial state.

In some embodiments, the cleaning liquid supply part 65 may sequentially add one or more cleaning liquids to the reaction tank 40 and discharge the cleaning liquid as waste liquid after soaking the reaction tank 40 for a predetermined time; the preset time here is, for example, 10 minutes or more. In the case of adding a cleaning liquid, the cleaning liquid supply part 65 first adds the cleaning liquid to the reaction tank 40 for soaking for a predetermined time and then discharges it as a waste liquid. In the cleaning with the addition of a plurality of cleaning liquids, the cleaning liquid supply part 65 first adds a first cleaning liquid to the reaction tank 40 to soak for a predetermined time, and then discharges it as a waste liquid; the cleaning liquid supply part 65 further adds the second cleaning liquid to the reaction cell 40 to soak for a predetermined time and then discharges it as a waste liquid. The cleaning liquid reacts with the pollutants adhered to the pool wall to decompose and fall off, so that the effect of enhancing cleaning is achieved. The cleaning solution may be a different kind of haemolysing agent.

In some embodiments, the cleaning liquid supply part 65 can also add one or more cleaning liquids to the reaction cell 40, and the reaction cell 40 is washed by the cleaning liquid.

Thus, the cleaning liquid supply part 65 may supply one or more cleaning liquids, alternately washing and soaking in order. In the deep cleaning mode, the cleaning liquid supply part 61 can add the cleaning liquid to the reaction tank 40 for washing, and further, the cleaning liquid washing and the cleaning liquid soaking can be alternately performed one or more times, for example, first cleaning liquid washing, second cleaning liquid soaking, and second cleaning liquid washing, on the basis that the cleaning liquid supply part 65 uses the cleaning liquid to wash the reaction tank 40; then, for example, a cleaning solution soaking is performed first, and then a cleaning solution rinsing is performed. Typically, the last cleaning is a cleaning fluid rinse.

(III) temporary cleaning mode

The temporary cleaning mode is a cleaning which is added temporarily and additionally in the test process, and is triggered when a specific condition is met, so that the temporary cleaning mode can be used as a supplement of the conventional cleaning mode. Therefore, in some embodiments, the controller 70 controls the washing assembly 60 to perform the temporary washing mode to wash the reaction cell 40 when a preset temporary washing condition is reached. The preset temporary cleaning condition is generally a supplement to the preset regular cleaning time of the deep cleaning mode, and when the sample detection device does not reach the preset regular cleaning time, the device judges that an additional temporary cleaning condition needs to be performed.

In some embodiments, the predetermined temporary cleaning conditions include one or more of:

(1) whether the number of tests for performing the specific protein reaction test in the reaction cell 40 reaches a set number is determined, and if so, the reaction cell 40 is temporarily cleaned once. Specifically, the controller 70 determines whether the number of times of the accumulated tests of the detection of the content of the specific protein performed in the reaction cell 40 reaches a set number of times after the deep cleaning mode/the temporary cleaning mode is performed on the reaction cell 40 last time, and if so, controls the cleaning assembly 60 to perform the temporary cleaning mode to clean the reaction cell 40.

(2) The designated measurement mode is generally defined as whether the measurement mode including the detection of the specific protein has reached a set number of times, and if so, the reaction cell 40 is temporarily cleaned once. Specifically, the controller 70 determines whether the accumulated number of times of the designated measurement mode performed in the reaction cell 40 reaches a set number of times since the last execution of the deep cleaning mode/temporary cleaning mode for the reaction cell 40, and if so, controls the cleaning assembly 60 to execute the temporary cleaning mode to clean the reaction cell 40. The condition (2) is similar to the condition (1).

(3) Whether the accumulation of the specific protein content in the detection result of each sample measured in the reaction tank 40 reaches a set value or not is determined, and if the accumulation reaches the set value, the reaction tank 40 is temporarily cleaned once. Specifically, the controller 70 determines whether an integrated value of the detection result of the specific protein content performed in the reaction cell 40 reaches a set value since the last execution of the deep cleaning mode/temporary cleaning mode for the reaction cell 40, and if so, controls the cleaning module 60 to execute the temporary cleaning mode to clean the reaction cell 40.

(4) Each sample is measured in the reaction tank 40 to obtain a detection result of the specific protein content, and then the number of samples with the specific protein content larger than a set value in the detection result is large, and if the number of samples is large, the reaction tank 40 is temporarily cleaned once. Specifically, the controller 70 determines whether the number of samples having a detection result greater than a set value among samples of the specific protein content detection performed in the reaction cell 40 reaches a set number after performing the deep cleaning mode/the temporary cleaning mode on the reaction cell 40 last time, and if so, controls the cleaning assembly 60 to perform the temporary cleaning mode to clean the reaction cell 40.

(5) Or collecting signals of appointed time points in the measuring process as judgment conditions; for example, blank voltage (signal detection voltage value when the cell body is filled with specific liquid, and diluent is generally used) is collected as a judgment condition; reading a blank voltage value every time a sample is tested; when the blank voltage meets the set condition, the pollution degree of the cell body reaches a certain level, and the continuous use may have a certain influence on the measured value, at this time, the reaction cell 40 may be temporarily cleaned once. Therefore, in some embodiments, the controller 70 obtains a blank voltage of the reaction cell, and when the blank voltage is within a preset range, the controller 70 controls the cleaning assembly 60 to perform a temporary cleaning mode to clean the reaction cell 40; the blank voltage is: when the reaction cell 40 contains a specific liquid, the controller 70 senses the transmitted or scattered light and converts the sensed light into a voltage by controlling the irradiation of the specific liquid in the reaction cell 40; the preset range of the blank voltage acquired by adopting the scattering principle is larger than a set voltage, and the preset range of the blank voltage acquired by adopting the transmission principle is smaller than the set voltage. In some embodiments, the particular liquid is a cleaning liquid or a cleaning liquid; the blank voltage is controlled to be collected after the controller 70 controls the normal cleaning mode to be performed to clean the reaction cell 40.

In the temporary cleaning mode, the cleaning process may be similar to the conventional cleaning mode or the deep cleaning mode. In some embodiments, in the temporary washing mode, the cleaning liquid supply part 65 adds one or more cleaning liquids to the reaction cell and discharges the cleaning liquid as waste liquid after soaking the reaction cell 40 for a preset time; and/or, the cleaning liquid supply part 61 washes the reaction cell with a cleaning liquid or a cleaning liquid. Further, the cleaning solution rinsing and the cleaning solution soaking can be alternately performed one or more times, for example, first, cleaning solution rinsing, then cleaning solution soaking, and then cleaning solution rinsing; then, for example, a cleaning solution soaking is performed first, and then a cleaning solution rinsing is performed. Typically, the last cleaning is a cleaning fluid rinse.

Since the temporary cleaning mode is a cleaning added as "temporary and extra" during the test process, in some examples, the temporary cleaning mode may be a simplified version of the deep cleaning mode, for example, the temporary cleaning mode may reduce the cleaning time, reduce the number of times of sucking and discharging the cleaning process, and reduce the type of cleaning solution compared to the deep cleaning mode.

In some embodiments, the time consumption of one regular cleaning mode is less than that of one temporary cleaning mode, and the time consumption of one temporary cleaning mode is less than or equal to that of one deep cleaning mode. For example, it takes about 10 seconds to perform the normal cleaning mode once, about 1 minute to perform the temporary cleaning mode once, and about 10 minutes to perform the deep cleaning mode once.

The foregoing are some of the descriptions of the sample testing device of the present application.

In some embodiments, a method of sample testing device is also disclosed, and the sample testing device referred to herein may be the sample testing device described in any of the embodiments of the present application. Referring to fig. 9, in some embodiments, the method of the sample testing apparatus includes the following steps:

step 100: and controlling to suck a sample to be detected and discharging the sample to be detected into a reaction pool for detecting the specific protein.

Step 110: and controlling the addition of the latex reagent for reaction to the reaction cell for detecting the specific protein to prepare a mixed sample solution.

Step 120: and controlling the irradiation of the mixed sample liquid to detect the content of the specific protein in the mixed sample liquid.

Steps 100 to 120 are the procedure for detecting a specific protein. It is understood that, when the blood sample to be tested is serum or plasma containing no blood cells, the above-mentioned mixed sample solution can be prepared by reacting a reagent for detecting a specific protein, such as a latex reagent, with such a blood sample for detecting a specific protein; when the blood sample to be tested is a whole blood sample containing blood cells, it is necessary to add a hemolytic agent to the whole blood sample to dissolve the blood cells and then add a specific protein detection reagent such as a latex reagent to prepare the above-mentioned mixed sample solution to perform detection of the specific protein. Of course, it will be understood by those skilled in the art that in some instances, it may be desirable to also add reagents such as buffers and/or diluents.

Step 300: and controlling to clean the reaction tank.

In some instances, the same blood sample may also be used for routine blood testing and for the detection of specific proteins, as described in more detail below. Referring to fig. 10, in some embodiments, the method of the sample testing apparatus includes the following steps:

step 200: and controlling to suck a sample to be detected, and respectively discharging one sample to be detected into the blood conventional reaction pool and the reaction pool for detecting the specific protein. For example, in some cases, after the sample needle draws a blood sample to be tested, a portion of the sample is discharged to a blood-based reaction cell, and the remaining sample is discharged to a reaction cell for specific protein detection.

Step 210: and controlling the addition of one or more hemolytic agents to the blood conventional reaction tank. This is done by treating the sample to be tested with a hemolytic agent. The reagent for routine blood test comprises one or more hemolytic agents.

Step 220: controlling the test of at least one item in the blood routine for the sample to be tested treated by the hemolytic agent. For example, the cell classification and/or counting of the sample to be tested in step 220 may include five classifications of WBC, WBC counting, and measurement of morphological parameters, and may also include measurement of HGB (hemoglobin), RBC (red blood cell), PLT (blood platelet) counting, and measurement of morphological parameters.

Step 230: and controlling the addition of the latex reagent for reaction to the reaction cell for detecting the specific protein to prepare a mixed sample solution.

Step 240: and controlling the irradiation of the mixed sample liquid to detect the content of the specific protein in the mixed sample liquid.

In the above process, the routine blood measurement of the sample is completed in steps 200 to 220. Step 200, step 230 and step 240 are performed to detect specific proteins.

Step 300: the control system performs cleaning on the reaction tank. In some cases, the blood reaction chamber may be cleaned. The process, method, mode and steps for washing the blood general reaction pool can be similar to those for washing the reaction pool, and are not described in detail herein.

The following describes in detail how step 300 performs the washing of the reaction cell.

In some embodiments, the step 300 of performing the washing on the reaction cell includes three washing modes, i.e., a normal washing mode, a temporary washing mode, and a deep washing mode, which are described below.

Normal cleaning mode

The normal wash mode is a normal wash of the reaction cell after each sample test is completed. In other words, each time the mixed sample solution in the reaction cell is detected, the reaction cell is controlled to perform a normal washing mode to wash the reaction cell and restore the reaction cell to a measurement ready state, so as to avoid influencing the detection result of the next sample, thereby enabling the reaction cell to perform the detection of the specific protein content of the next sample. The conventional cleaning mode can be to control the washing of the reaction pool by using a cleaning liquid, and the cleaning liquid can be a diluent and the like. Of course, the conventional cleaning mode can also be matched with a cleaning liquid, for example, the cleaning liquid is added into the reaction to soak and clean the reaction cell, and the cleaning liquid can be a hemolytic agent.

Thus, in some embodiments, step 300 controls performing a wash on the reaction cell, including: and after the mixed sample liquid in the reaction tank is controlled to be detected each time, controlling to execute a conventional cleaning mode to clean the reaction tank so as to enable the reaction tank to detect the specific protein content of the next sample. In some embodiments, the normal cleaning mode includes: the washing of the reaction tank with the washing liquid is controlled, and specifically, the washing liquid may be controlled to be added to the reaction tank in an eccentric direction, for example, in a tangential direction along the wall of the reaction tank, so that a swirling state is formed when the washing liquid is added to the reaction tank. Further, when controlling to use the washing liquid to right the reaction tank washes, still constantly will washing liquid in the reaction tank is discharged as the waste liquid, perhaps, control uses the washing liquid to right the reaction tank washes, still constantly will washing liquid in the reaction tank discharges and retrieves and regard as again as right the washing liquid that the reaction tank washed usefulness, whirl duration can be than longer like this, and the cleaning performance can be better. In some examples, under the condition of using the cleaning solution for flushing, a cleaning solution such as an LH hemolytic agent can be added into the reaction tank under control, and the cleaning solution is discharged as a waste liquid after soaking the reaction tank for a preset time; the cleaning liquid reacts with the pollutants adhered to the pool wall to decompose and fall off, so that the effect of enhancing cleaning is achieved. The cleaning solution rinsing and the cleaning solution soaking can be alternately performed one or more times, for example, the cleaning solution rinsing is performed once, the cleaning solution soaking is performed once again, and the cleaning solution rinsing is performed once again; then, for example, a cleaning solution soaking is performed first, and then a cleaning solution rinsing is performed. Typically, the last cleaning is a cleaning fluid rinse.

Generally, although most of the pollutants can be cleaned by the conventional cleaning mode, few pollutants may remain to adhere to the wall of the reaction cell, and thus the pollutants in the reaction cell may be accumulated after a long time of use, so that the pollutants in the reaction cell may affect the accuracy of the detection result sufficiently, and even the reaction cell may be irreversibly damaged, for example, the pollutants adhered to the wall of the reaction cell cannot be removed by soaking the reaction cell for a long time with a cleaning solution, or even manually scraping the pollutants. In consideration of this situation, the present application is also provided with two cleaning modes, a temporary cleaning mode and a deep cleaning mode.

(II) deep cleaning mode

Thus, in some embodiments, step 300 controls performing a wash on the reaction cell, including: and when a preset regular cleaning time is reached, controlling to execute a deep cleaning mode to clean the reaction tank. The preset periodic cleaning opportunities include any one or more of: the method comprises the following steps of starting up the sample detection device every day, stopping up the sample detection device every day, enabling the sample detection device to enter the sleep state, enabling the sample detection device to exit the sleep state, and setting a regular cleaning time point or receiving a deep cleaning starting command. In some embodiments, the deep cleaning mode comprises: and controlling one or more cleaning liquids to be added into the reaction tank, and discharging the reaction tank as waste liquid after soaking for a preset time. Further, the deep cleaning mode may further include: and controlling to flush the reaction tank by using a cleaning solution or the cleaning solution. When the cleaning liquid or the cleaning liquid is used to wash the reaction tank in the deep cleaning mode, reference may be made to the conventional cleaning mode in which the cleaning liquid is added eccentrically, particularly, tangentially to the wall of the reaction tank, so that the cleaning liquid forms a swirling flow state when added into the reaction tank, and further, the deep cleaning mode further includes: continuously discharging the cleaning solution/the cleaning solution in the reaction tank as waste liquid while controlling the reaction tank to be flushed by the cleaning solution/the cleaning solution, or continuously discharging the cleaning solution/the cleaning solution in the reaction tank and recovering the cleaning solution/the cleaning solution as the cleaning solution/the cleaning solution for flushing the reaction tank while controlling the reaction tank to be flushed by the cleaning solution/the cleaning solution; like this, the liquid of washing usefulness forms the whirl duration in the reaction tank can be longer, and the cleaning performance can be better.

In the normal washing mode, a hemolytic agent having a high cleaning ability may be used as the cleaning liquid, and in the deep washing mode, a hemolytic agent having a high cleaning ability may be used as the cleaning liquid.

In some examples, in the deep cleaning mode, the cleaning solution rinsing and the cleaning solution soaking can be alternately performed one or more times, for example, first cleaning solution rinsing, then cleaning solution soaking, and then cleaning solution rinsing; then, for example, a cleaning solution soaking is performed first, and then a cleaning solution rinsing is performed. Typically, the last cleaning is a cleaning fluid rinse.

(III) temporary cleaning mode

The temporary washing mode is generally performed during the current test, and the sample testing apparatus is triggered by the preset temporary washing condition, so that the reaction cell is additionally washed based on the conventional washing mode. The temporary cleaning mode may be similar to the normal cleaning mode or the deep cleaning mode, but since the temporary cleaning mode is a cleaning additionally added in the test process, the temporary cleaning mode may reduce the time in order to avoid the influence on the normal test, for example, the temporary cleaning mode may be similar to the deep cleaning mode, but compared to the deep cleaning mode, the temporary cleaning mode may reduce the cleaning time, reduce the suction and discharge times in the cleaning process, reduce the types of cleaning liquids used, and the like, taking the cleaning time as an example, the deep cleaning mode may be 10 minutes, and the temporary cleaning mode may be about 1 minute.

Thus, in some embodiments, step 300 controls performing a wash on the reaction cell, including: and when a preset temporary cleaning condition is reached, controlling to execute a temporary cleaning mode to clean the reaction tank.

(1) and (3) whether the testing frequency for detecting the specific protein reaction in the reaction tank reaches the set frequency or not, and if so, temporarily cleaning the reaction tank once. Specifically, it is determined whether the number of times of the accumulated tests for the detection of the content of the specific protein performed in the reaction cell reaches a set number of times after the deep cleaning mode/temporary cleaning mode is performed on the reaction cell 40 since the last time, and if so, the temporary cleaning mode is controlled to be performed to clean the reaction cell.

(2) The designated measurement mode is generally that whether the measurement mode including the detection of the specific protein reaches a set number of times, and if so, the reaction cell is temporarily cleaned once. Specifically, after the deep cleaning mode/temporary cleaning mode is executed on the reaction tank last time, whether the accumulated times of the specified measurement mode performed on the reaction tank reaches a set time or not is judged, and if the accumulated times reaches the set time, the temporary cleaning mode is controlled to be executed to clean the reaction tank. The condition (2) is similar to the condition (1).

(3) And (3) whether the accumulation of the specific protein content in the detection result of each sample measured in the reaction tank reaches a set value or not is detected, and if so, the reaction tank is temporarily cleaned once. Specifically, after the deep cleaning mode/temporary cleaning mode is executed on the reaction tank last time, whether the accumulated value of the detection result of the specific protein content in the reaction tank reaches a set value is judged, and if the accumulated value reaches the set value, the temporary cleaning mode is controlled to be executed to clean the reaction tank.

(4) And measuring each sample in the reaction tank to obtain a detection result of the specific protein content, then determining the number of the samples with the specific protein content larger than a set value in the detection result, and if the number of the samples is larger than the set value, temporarily cleaning the reaction tank once. Specifically, after the deep cleaning mode/temporary cleaning mode is executed on the reaction tank for the last time, whether the number of samples with the detection result larger than a set value among samples for detecting the content of the specific protein in the reaction tank reaches a set number is judged, and if the number of samples reaches the set number, the temporary cleaning mode is controlled to be executed to clean the reaction tank.

(5) Or collecting signals of appointed time points in the measuring process as judgment conditions; for example, blank voltage (signal detection voltage value when the cell body is filled with specific liquid, and diluent is generally used) is collected as a judgment condition; reading a blank voltage value every time a sample is tested; when the blank voltage meets the set condition, the pollution degree of the cell body reaches a certain level, the continuous use may have certain influence on the measured value, and at the moment, the reaction cell can be temporarily cleaned once. Therefore, in some embodiments, a blank voltage of the reaction cell is obtained, and when the blank voltage is within a preset range, the temporary cleaning mode is controlled to be executed to clean the reaction cell; the blank voltage is: when the reaction cell is filled with specific liquid, the specific liquid in the reaction cell is irradiated by control, and transmitted or scattered light is induced and converted into voltage; for example, the preset range of the blank voltage acquired by the scattering principle is greater than a set voltage, and the preset range of the blank voltage acquired by the transmission principle is less than a set voltage. In some embodiments, the particular liquid is a cleaning liquid or a cleaning liquid; and when the reaction tank is controlled to execute a conventional cleaning mode to clean the reaction tank, the blank voltage is controlled to be collected.

As described above, in the temporary cleaning mode, the cleaning process may be similar to the conventional cleaning mode or the deep cleaning mode. In some embodiments, in the temporary cleaning mode, one or more cleaning liquids are controlled to be added into the reaction tank, and the reaction tank is soaked for a preset time and then is discharged as waste liquid; and/or controlling the washing of the reaction tank by using a cleaning solution or a cleaning solution. Further, the cleaning solution rinsing and the cleaning solution soaking can be alternately performed one or more times, for example, first, cleaning solution rinsing, then cleaning solution soaking, and then cleaning solution rinsing; then, for example, a cleaning solution soaking is performed first, and then a cleaning solution rinsing is performed. Typically, the last cleaning is a cleaning fluid rinse.

One arrangement of the three cleaning modes can be carried out by controlling to execute a conventional cleaning mode to clean the reaction tank after controlling to detect the mixed sample liquid in the reaction tank each time, so that the reaction tank can carry out the detection of the specific protein content of the next sample; when mixed sample liquid in the reaction tank is detected each time, whether a preset regular cleaning time is reached is also judged, if the preset regular cleaning time is reached, the deep cleaning mode is controlled to be executed to clean the reaction tank, if the preset regular cleaning time is not reached, whether a preset temporary cleaning condition is reached is further judged, if the preset temporary cleaning condition is reached, the temporary cleaning mode is controlled to be executed to clean the reaction tank, and if the preset temporary cleaning condition is not reached, the sample detection equipment is in a ready state, and the detection of specific protein of the next sample can be carried out or started.

By the sample detection device and the method thereof, the reaction cell can be kept in a cleaning state for a long time. Specifically, according to the sample detection device and the method thereof, besides a conventional cleaning mode, a temporary cleaning mode and a deep cleaning mode are introduced, pollutants accumulated in the using process can be thoroughly removed, the state of the cell body is recovered to the initialization purpose, the accuracy of the detection result is maintained, and meanwhile, the cell body can be kept in a good state for a long time, so that the cell wall does not need to be wiped and the like manually and periodically. In addition, the detection device and the detection method of the application also prolong the service life of the reaction tank and reduce the use cost of instruments, because the reaction tank in the prior art often needs to be replaced for one time at regular intervals, such as 1 to 2 months, because many tank bodies in the prior art are possibly not thoroughly cleaned, so that the tank bodies are completely damaged and irreversibly polluted, even if the pollutants on the tank wall cannot be removed by means of manual wiping and the like, the tank bodies can only be replaced. In addition, the sample detection device and the method thereof can reduce the time consumed in a cleaning process, such as a conventional cleaning mode, and corresponding cleaning reagents in a normal test process due to the introduction of a temporary cleaning mode and a deep cleaning mode, particularly a temporary cleaning mode; for example, the cleaning time in the normal test process can be reduced from 20 seconds to 30 seconds to about 10 seconds at present, and the test speed is improved.

Reference is made herein to various exemplary embodiments. However, those skilled in the art will recognize that changes and modifications may be made to the exemplary embodiments without departing from the scope hereof. For example, the various operational steps, as well as the components used to perform the operational steps, may be implemented in differing ways depending upon the particular application or consideration of any number of cost functions associated with operation of the system (e.g., one or more steps may be deleted, modified or incorporated into other steps).

In the above embodiments, the implementation may be wholly or partially realized by software, hardware, firmware, or any combination thereof. Additionally, as will be appreciated by one skilled in the art, the principles herein may be reflected in a computer program product on a computer readable storage medium, which is pre-loaded with computer readable program code. Any tangible, non-transitory computer-readable storage medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-to-ROM, DVD, Blu-Ray discs, etc.), flash memory, and/or the like. These computer program instructions may be loaded onto a general purpose computer, special purpose computer, or other programmable data processing apparatus to produce a machine, such that the instructions which execute on the computer or other programmable data processing apparatus create means for implementing the functions specified. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including means for implementing the function specified. The computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified.

While the principles herein have been illustrated in various embodiments, many modifications of structure, arrangement, proportions, elements, materials, and components particularly adapted to specific environments and operative requirements may be employed without departing from the principles and scope of the present disclosure. The above modifications and other changes or modifications are intended to be included within the scope of this document.

The foregoing detailed description has been described with reference to various embodiments. However, one skilled in the art will recognize that various modifications and changes may be made without departing from the scope of the present disclosure. Accordingly, the disclosure is to be considered in an illustrative and not a restrictive sense, and all such modifications are intended to be included within the scope thereof. Also, advantages, other advantages, and solutions to problems have been described above with regard to various embodiments. However, the benefits, advantages, solutions to problems, and any element(s) that may cause any element(s) to occur or become more pronounced are not to be construed as a critical, required, or essential feature or element of any or all the claims. As used herein, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, system, article, or apparatus. Furthermore, the term "coupled," and any other variation thereof, as used herein, refers to a physical connection, an electrical connection, a magnetic connection, an optical connection, a communicative connection, a functional connection, and/or any other connection.

Those skilled in the art will recognize that many changes may be made to the details of the above-described embodiments without departing from the underlying principles of the invention. Accordingly, the scope of the invention should be determined only by the claims.

Claims

1. A sample testing device, comprising:

the sample mechanism is used for providing a sample to be tested;

2. The sample testing device of claim 1, wherein the reaction cell is provided with a waste port and a first wash port;

3. The sample testing device of claim 2, wherein the first cleaning port is disposed tangentially to the wall of the reaction cell.

4. The specimen-detecting apparatus according to claim 2 or 3, wherein the washing liquid supply part further includes a recovery channel having one end connected to the waste liquid discharge port and the other end connected to the first washing port, for recovering the washing liquid discharged from the waste liquid discharge port as a washing liquid for washing the reaction cuvette.

5. The sample testing device of claim 2, wherein the reaction cell is provided with a second wash port; the cleaning liquid supply member includes a cleaning liquid supply passage connected to the second cleaning port of the reaction cell, the cleaning liquid supply member being for supplying the cleaning liquid to the reaction cell through the cleaning liquid supply passage;

6. The sample testing device of claim 5, wherein the second cleaning port is disposed tangentially to the wall of the reaction cell.

7. The sample testing device of claim 2, wherein said cleaning liquid supply unit reuses said reagent mechanism for supplying one or more hemolytic agents as said cleaning liquid to said reaction cell during washing.

8. The sample testing device of claim 1, wherein the time consumption of one regular wash mode is less than the time consumption of one interim wash mode, and the time consumption of one interim wash mode is less than or equal to the time consumption of one deep wash mode.

9. A method of sample testing apparatus, comprising:

10. A method of sample testing apparatus, comprising:

11. The method of claim 9 or 10, wherein the normal washing mode comprises:

and controlling to use a cleaning solution to flush the reaction tank.

12. The method of claim 11, wherein the controlling flushing the reaction cell with a cleaning solution comprises:

13. The method of claim 12, wherein the normal wash mode further comprises:

14. The method of claim 11, wherein the normal wash mode further comprises:

15. The method of claim 14, wherein the cleaning solution comprises one or more hemolysing agents.

16. The method of claim 9 or 10, wherein the deep cleaning mode comprises:

17. The method of claim 16, wherein the deep cleaning mode further comprises:

18. The method of claim 17, wherein the deep cleaning mode further comprises:

19. The method of claim 9, 10, 16, 17 or 18, wherein the preset periodic cleaning opportunity comprises any one or more of:

when the sample detection equipment is started up every day;

when the sample detection equipment is shut down every day;

when the sample detection equipment enters dormancy;

when the sample detection device exits from sleep;

a preset periodic cleaning time point;

a deep cleaning start command is received.

20. The method of claim 9 or 10, wherein the temporary cleaning mode comprises:

21. The method of claim 16, 17, 18 or 20, wherein the cleaning solution comprises one or more hemolysing agents.

22. A method according to claim 9, 10 or 20, wherein the pre-set temporary cleaning conditions include any one or more of:

acquiring blank voltage of a reaction tank, and controlling to execute a temporary cleaning mode to clean the reaction tank when the blank voltage is within a preset range; the blank voltage is: when the reaction cell is filled with specific liquid, the specific liquid in the reaction cell is irradiated by control, and transmitted or scattered light is induced and converted into voltage.

23. The method of claim 22, wherein the specific liquid is a cleaning liquid or a cleaning liquid; and when controlling to execute a normal cleaning mode to clean the reaction cell, controlling to collect the blank voltage.