CN113862124A - Probiotic sampling instrument and sampling method thereof - Google Patents
Probiotic sampling instrument and sampling method thereof Download PDFInfo
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- CN113862124A CN113862124A CN202110954047.1A CN202110954047A CN113862124A CN 113862124 A CN113862124 A CN 113862124A CN 202110954047 A CN202110954047 A CN 202110954047A CN 113862124 A CN113862124 A CN 113862124A
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- 238000005070 sampling Methods 0.000 title claims abstract description 321
- 239000006041 probiotic Substances 0.000 title claims abstract description 47
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 47
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000001514 detection method Methods 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims description 17
- 239000004033 plastic Substances 0.000 claims description 6
- 230000001681 protective effect Effects 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000005086 pumping Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 description 13
- 230000009471 action Effects 0.000 description 9
- 241000233866 Fungi Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000005452 bending Methods 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 241000193171 Clostridium butyricum Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/07—Dosage or metering devices therefore
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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Abstract
The invention belongs to the technical field of probiotic sampling, and particularly relates to a probiotic sampling instrument and a sampling method thereof, wherein the probiotic sampling instrument comprises a sampling sleeve, a piston hole is formed in the sampling sleeve, a plurality of sampling chambers which are not communicated with each other are formed in the sampling sleeve, a piston rod is slidably mounted in the piston hole, a sampling handle is mounted at the upper end of the piston rod, a magnet I is mounted outside the piston rod, a magnet II is mounted in the sampling chamber, a piston is mounted on the lower surface of the magnet II, the magnet I and the magnet II are mutually attracted, a base is fixedly mounted on the lower surface of the sampling sleeve, and sampling pipes which are in one-to-one correspondence with and communicated with the sampling chambers are fixedly mounted on the lower surface of the base; the sampling device has a simple structure, can realize one-time multipoint sampling, improves the sampling efficiency and the sample detection accuracy, and prevents external bacteria from entering the sampling chamber to pollute the sample, because only one opening of the sampling tube in the sampling chamber is in contact with the sample.
Description
Technical Field
The invention belongs to the technical field of probiotic sampling, and particularly relates to a probiotic sampling instrument and a sampling method thereof.
Background
The probiotics is a general name of active beneficial microorganisms which are beneficial to a host, can generate definite health efficacy by being planted in the intestinal tract and the reproductive system of a human body, thereby improving the microbial ecological balance of the host and exerting beneficial effects on the intestinal tract. The beneficial bacteria or fungi in human bodies and animal bodies are mainly as follows: clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes, saccharomycetes and the like, wherein after the processing of the probiotics is finished, the probiotics product needs to be subjected to sampling inspection, whether the content and activity level of the probiotics in the product meet the national standard or not is determined, unqualified products are removed, the product sold in the hands of consumers can have a good effect, and a sampling instrument is needed to perform sampling operation on the probiotics at the moment.
Some technical solutions for sampling probiotics also exist in the prior art, for example, chinese patent with application number CN201921152157.0 discloses a device for sampling probiotic powder, which includes a casing and a sampling tube, the sampling tube is connected with the bottom of the sleeve, a movable rod is connected in the sleeve in a sliding way, the top of the movable rod is connected with a transmission rack, the outer wall of the transmission rack is engaged and connected with a pinion, the top of the sleeve is connected with a supporting frame, a rotating shaft is connected in the supporting frame in a rotating way, the pinion is connected to the outer wall of the rotating shaft, one end of the rotating shaft, which is far away from the pinion, penetrates through the supporting frame and is connected with a knob, one end of the movable rod, which is far away from the transmission rack, is connected with a piston which is connected in a sampling pipe in a sliding way, the bottom of sampling tube is connected with the sample connection, just the sample connection outer wall is connected with this technique of check valve. According to the technical scheme, the rack is driven to move up and down by controlling the rotation of the gear through the meshing between the gear and the rack, so that the absorption amount of probiotics is accurately controlled, but the scheme has the following problems that only one sampling port is arranged, so that a sample at a single position can be sampled at each time during sampling, the representativeness of the sample is general, the actual condition of the sample cannot be fully presented, and simultaneously, the sampled sample is easily polluted by external sundry bacteria after sampling and before detection.
Disclosure of Invention
The invention provides a probiotic sampling instrument and a sampling method thereof, aiming at making up for the defects of the prior art and solving the problems that the representative of a sample is general and the actual condition of the sample cannot be fully presented because only a certain sample can be sampled during sampling, and simultaneously the sampled sample is easily polluted by external bacteria after sampling and before detection.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a probiotic sampling instrument, which comprises a sampling sleeve, wherein a piston hole is formed in the sampling sleeve, the center line of the piston hole is coincided with the center line of the sampling sleeve, a plurality of sampling chambers are formed in the sampling sleeve and are uniformly distributed along the circumferential direction, the sampling chambers are not communicated with each other, a piston rod is arranged in the piston hole in a sliding manner, a sampling handle is fixedly arranged at the upper end of the piston rod, a first magnet is fixedly arranged on the outer surface of the piston rod and is positioned at one side close to the lower end of the piston rod, a second magnet is arranged in the sampling chamber in a sliding manner, a piston is fixedly arranged on the lower surface of the second magnet and is mutually attracted, a base is fixedly arranged on the lower surface of the sampling sleeve, sampling pipes are fixedly arranged on the lower surface of the base, and correspond to the sampling chambers one another, the sampling tube is communicated with the interior of the sampling chamber;
when the sampling device works, the sampling tube is stretched into a liquid probiotic product, the sampling handle is pulled upwards to drive a piston rod fixedly connected with the sampling handle to slide upwards together, a first magnet is fixedly arranged on the outer surface of the piston rod, a second magnet is slidably arranged in the sampling chamber, and the first magnet and the second magnet attract each other, so that when the piston rod slides upwards, the first magnet moves upwards together with the piston rod, the second magnet also moves upwards synchronously to drive a piston fixedly connected with the lower surface of the second magnet to move upwards together, so that a pressure difference exists between the inside of the sampling chamber and the liquid probiotic product, and under the action of the pressure difference, a sample passes through the sampling tube to be extracted from the sampling chamber, and simultaneously, because attraction exists between the first magnet and the second magnet, when the piston rod moves upwards, the upwards sliding distances of the second magnet in each sampling chamber are the same, therefore, the air pressure difference between each sampling chamber and the liquid probiotic product is the same, so that the amount of samples extracted from each sampling chamber is the same in each sampling process, because a plurality of sampling chambers which are uniformly distributed along the circumferential direction are arranged in the sampling sleeve, and each sampling chamber corresponds to each sampling pipe one by one, in one sampling process, different positions in the same liquid probiotic product can be sampled simultaneously, so that a sampler can collect a plurality of samples positioned at different positions in one sampling process, the detection precision in the subsequent probiotic detection is improved, the detection error caused by insufficient sample amount is reduced, the artificial error caused by the sampling operation of an operator for a plurality of times in the sampling process for improving the detection precision is reduced, the sampling efficiency is improved, and meanwhile, the sampling chambers are not communicated with each other, and only one inlet of the sampling tube is arranged in the sampling chamber, so that the sampling chamber is in a relatively sealed environment, and the possibility of pollution to the sample caused by the fact that external bacteria are mixed into the sampling chamber is reduced.
Preferably, a cavity is formed in the base, the cavity corresponds to the sampling chamber one by one, a conical block is fixedly mounted on the upper surface of the cavity, a hose is mounted on the outer side of the conical block, the hose is fixedly connected to the upper surface of the cavity, the hose is communicated with the sampling chamber, a magnet III is slidably mounted on the outer surface of the hose, the magnet III and the magnet II repel each other, a spring is fixedly connected to the lower surface of the magnet III, and the other end of the spring is fixedly connected to the lower surface of the cavity;
when the sampling handle is pulled upwards, the magnet II moves upwards at the same time under the action of the attraction force of the magnet II to the magnet III, the distance between the magnet II and the magnet III is increased, the repulsion force between the magnet II and the magnet III is reduced, the pressure of the magnet III to the spring is reduced, and the spring is restored to the natural state upwards, at this moment, under the spring to the ascending elasticity effect of magnet three, three upward movements of magnet, after accomplishing the sample, three upward movements of magnet are to toper piece department, passageway in the hose is totally enclosed under the cooperation of magnet three and toper piece, make the sampling chamber be in a totally enclosed environment, thereby avoid external miscellaneous fungus to pollute the sample in the sampling chamber after the sample is accomplished, the sample after having also avoided the sample to accomplish simultaneously is extruded the sampling chamber under the effect of atmospheric pressure and leads to the sample volume not enough and the sample to flow out the back, cause other utensil pollutes.
Preferably, the piston rod is internally provided with a mounting hole, the piston rod is provided with a first sliding groove, the length direction of the first sliding groove is the same as that of the piston rod, the number of the sliding grooves is two, the first sliding grooves are symmetrically distributed on the piston rod, the piston rod is provided with a second sliding groove, the second sliding grooves are symmetrically distributed at the upper end and the lower end of the first sliding groove, the joint of the first sliding groove and the second sliding groove is located at the middle position of the second sliding groove, the second sliding groove is communicated with the first sliding groove, the second sliding groove and the first sliding groove are perpendicular to each other, a fixing rod is slidably mounted in the mounting hole, a strut is fixedly mounted at the upper end of the fixing rod, the strut penetrates through the second sliding groove and is fixedly connected to the inner wall of the piston hole, and the lower end of the fixing rod is connected with the base through threads;
when the sampling device works, the sampling tube extends into a liquid probiotic product, the sampling handle is rotated, so that the piston rod is driven to rotate, a strut penetrating through a chute II at the upper end of the chute I and fixedly connected to the inner wall of a piston hole and a chute II at the upper end of the chute I rotate relatively, the strut rotates relatively to a position coinciding with the central line of the chute I, then the sampling handle is pulled upwards, so that the strut enters the chute I, the piston rod moves upwards to drive the magnet to move upwards, further the magnet II and the piston fixedly connected to the lower surface of the magnet II move upwards together, after sampling is completed, the sampling handle is rotated again, so that the strut and the chute II at the lower end of the chute I rotate relatively, further the strut enters the chute II to be fixed, the sampled sample is prevented from being discharged again under the action of air pressure difference in the sampling chamber due to unfixed piston rod after sampling is completed, simultaneously after the sampling operation is accomplished, because pass through threaded connection between base and the dead lever to make the base can dismantle, consequently after the sample is accomplished, dismantle the base and carry out thorough washing and sterilization process to the sample appearance, thereby guarantee that the sample appearance is in sterile condition when carrying out the sampling operation at every turn.
Preferably, one end of the hose, which is far away from the conical block, is a spiral hose, a rubber layer is fixedly arranged on the lower surface of the base, a sampling tube is fixedly arranged on the lower surface of the rubber layer, and the sampling tube penetrates through the rubber layer and is fixedly connected with the spiral hose;
the during operation, because the one end that the toper piece was kept away from to the hose is the spiral hose, and surface fixed mounting has the rubber layer under the base, the sampling tube is fixed through the extrusion on rubber layer, therefore when sampling operation, the staff can manually lead the sampling tube motion, and then adjust the sampling position and the sampling depth of sampling tube in liquid probiotic product, make the sample appearance can take a sample to the sample of different positions and degree of depth respectively, and then the scope of taking a sample has been improved, avoided the problem that sampling operation personnel need repeated many times to take a sample when taking a sample to the sample of different positions and degree of depth, the efficiency of sample has been improved, reduce the possibility of miscellaneous fungus pollution, and simultaneously, owing to can carry out sampling operation to the sample of different positions and degree of depth, also make the randomness of taking a sample improve, make the testing result after the sample more accurate effective.
Preferably, the sampling tube is made of a hard plastic tube;
the during operation, after sampling operation, because the material of sampling tube is the stereoplasm plastic tubing, therefore the sampling tube has elasticity, can take place bending deformation after receiving the extrusion, thereby make after the sample is accomplished, through upwards extruding the sampling tube, thereby make it upwards take place bending deformation and accomodate in the cavity, use aseptic protective sheath simultaneously with the sampling tube still expose outside position cover, the direct exposed outside position of sampling tube has been reduced, avoided the sampling tube to lead to the sampling tube to be contaminated by miscellaneous fungus with external contact after the sample is accomplished, thereby cause the pollution to next sample, the operating personnel is hindered to the mistake directly exposing outside simultaneously also avoided the sampling tube.
A probiotic sampling method is suitable for the probiotic sampling instrument, and comprises the following steps:
s1, inserting a sampling tube at the lower end of the sampler into a sample to be sampled, and adjusting the position of the sampling tube;
s2, rotating the sampling handle, rotating the piston rod to rotate the strut to the center of the first sliding groove, pulling the sampling handle to drive the piston rod and the first magnet on the piston rod to move upwards, so as to drive the second magnet and the piston to move upwards together, and pumping the sample into the sampling chamber;
s3, after sampling is completed, the repulsive force between the second magnet and the third magnet is reduced, the spring returns to the natural state upwards to drive the third magnet to move upwards, and the third magnet is matched with the conical block to extrude the hose together to seal the sampling chamber;
s4, rotating the sampling handle to enable the pillar to be located in the second sliding groove, fixing the piston rod to complete the sampling process, and then taking out the sample in the sampling chamber for detection or storage operation;
s5, after the operation of S3 is completed, the base is taken down, and the sampling instrument is cleaned and sterilized to be in an aseptic state when being used next time;
s6, after cleaning and sterilization, the sampling tube is upwards extruded, the sampling tube is stored in the cavity, then the base is covered with the sterile protective sleeve, and the sampler is stored in a dry and sterile environment for next use.
The invention has the following beneficial effects:
1. according to the probiotic sampling instrument, the plurality of sampling chambers which are not communicated with each other are arranged, so that the sampling instrument can sample different positions of the same sample at one time during sampling, multiple samples are collected, the phenomenon that the detection result is inaccurate due to single detection sample is avoided, and meanwhile, the moving distances of the piston in each sampling chamber are the same in each sampling process through the two magnets which are arranged outside the piston rod and in the sampling chambers and are mutually attracted, so that the air pressure difference generated by each sampling chamber is the same, and the number of samples collected in each sampling chamber is the same in each sampling process.
2. According to the probiotic sampling instrument, the conical block, the hose magnet III and the spring are arranged, and the magnet III and the magnet II are mutually repelled, so that the magnet III can downwards press the spring under the action of the repulsive force when sampling is not started, the hose and the sampling chamber are in a communicated state, the magnet II is arranged above the sampling chamber after sampling is completed, the repulsive force between the magnet II and the magnet III disappears, the magnet III upwards moves under the action of the elastic force of the spring to extrude a pipeline in the hose, the hose is completely sealed by matching with the conical block, and the sampling chamber is in a completely sealed state, so that the pollution of external bacteria to a sample is avoided, and the quality of the sample is influenced.
3. According to the probiotic sampling instrument, the spiral hose is connected with the sampling tube, so that the position and the depth of the sampling tube can be adjusted in the sampling process, samples at different positions and depths can be sampled by the sampling instrument, meanwhile, the sampling tube is made of a hard plastic tube and has flexibility and can be bent and deformed, and the sampling tube can be deformed and bent to be accommodated in the cavity by matching with the arrangement of the rubber layer, so that the length of the sampling tube exposed outside is changed, and the sampling tube is prevented from being polluted by the outside and being exposed outside too long to hurt sampling operators by mistake.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1 at A;
FIG. 3 is an enlarged view of a portion of FIG. 1 at B;
FIG. 4 is an enlarged view of a portion of FIG. 1 at C;
FIG. 5 is an enlarged view of a portion of FIG. 1 at D;
FIG. 6 is a flow chart of a sampling method of the sampler;
in the figure: the sampling device comprises a sampling sleeve 1, a piston hole 11, a sampling chamber 2, a magnet II 21, a piston 22, a piston rod 3, a sliding groove I31, a magnet I32, a sampling handle 33, a mounting hole 34, a sliding groove II 35, a base 4, a cavity 41, a conical block 42, a hose 43, a magnet III 44, a spring 45, a spiral hose 46, a fixing rod 5, a support column 51, a rubber layer 6 and a sampling tube 7.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 6, the probiotic sampling instrument of the present invention comprises a sampling sleeve 1, wherein a piston hole 11 is formed in the sampling sleeve 1, a center line of the piston hole 11 coincides with a center line of the sampling sleeve 1, a plurality of sampling chambers 2 are formed in the sampling sleeve 1, the sampling chambers 2 are uniformly distributed along a circumferential direction, the sampling chambers 2 are not communicated with each other, a piston rod 3 is slidably installed in the piston hole 11, a sampling handle 33 is fixedly installed at an upper end of the piston rod 3, a first magnet 32 is fixedly installed on an outer surface of the piston rod 3, the first magnet 32 is located at one side close to a lower end of the piston rod 3, a second magnet 21 is slidably installed in the sampling chambers, a piston 22 is fixedly installed on a lower surface of the second magnet 21, the first magnet 32 and the second magnet 21 are mutually attracted, and a base 4 is fixedly installed on a lower surface of the sampling sleeve 1, the lower surface of the base 4 is fixedly provided with sampling tubes 7, the sampling tubes 7 correspond to the sampling chambers 2 one by one, and the sampling tubes 7 are communicated with the insides of the sampling chambers 2;
when the sampling device works, the sampling tube 7 is stretched into a liquid probiotic product, the sampling handle 33 is pulled upwards to drive the piston rod 3 fixedly connected with the sampling handle 33 to slide upwards together, the magnet I32 is fixedly arranged on the outer surface of the piston rod 3, the magnet II 21 is slidably arranged in the sampling chamber 2, and the magnet I32 and the magnet II 21 attract each other, so that when the piston rod 3 slides upwards, the magnet I32 moves upwards together with the piston rod 3, the magnet II 21 also starts to move upwards synchronously, and further the piston 22 fixedly connected on the lower surface of the magnet II 21 is driven to move upwards together, so that a pressure difference exists between the inside of the sampling chamber 2 and the inside of the liquid probiotic product, and under the action of the pressure difference, a sample is extracted from the sampling chamber 2 through the sampling tube 7, meanwhile, because the attraction exists between the magnet I32 and the magnet II 21, when the piston rod 3 moves upwards, the upward sliding distances of the magnet II 21 in each sampling chamber 2 are the same, so that the air pressure difference between each sampling chamber 2 and the liquid probiotic product is the same, the sample amount extracted in each sampling chamber is the same in each sampling process, and because a plurality of sampling chambers 2 which are uniformly distributed along the circumferential direction are arranged in the sampling sleeve 1 and the sampling chambers 2 and the sampling pipes 7 are in one-to-one correspondence, in one sampling process, the sampling operation can be simultaneously carried out on different positions in the same liquid probiotic product, so that a sampler can collect a plurality of samples positioned at different positions in one sampling process, the detection precision in the subsequent probiotic detection is improved, the detection error caused by insufficient sample amount is reduced, and the artificial error caused by the sampling operation of an operator for improving the detection precision in the sampling process is reduced, the sampling efficiency is improved, simultaneously because each other do not communicate between the sampling chamber 2, and only have 7 imports of sampling tube in the sampling chamber 2 for be in a relatively sealed environment in the sampling chamber 2, reduced the possibility that external miscellaneous fungus sneaks into in the sampling chamber 2 and causes the pollution to the sample.
As an embodiment of the invention, a cavity 41 is formed in the base, the cavity 41 corresponds to the sampling chamber 2 one by one, a tapered block 42 is fixedly mounted on the upper surface of the cavity 41, a hose 43 is mounted on the outer side of the tapered block 42, the hose 43 is fixedly connected to the upper surface of the cavity 41, the hose 43 is communicated with the sampling chamber 2, a third magnet 44 is slidably mounted on the outer surface of the hose 43, the third magnet 44 and the second magnet 21 repel each other, a spring 45 is fixedly connected to the lower surface of the third magnet 44, and the other end of the spring 45 is fixedly connected to the lower surface of the cavity 41;
when the sampling handle 33 is not stretched, the second magnet 21 in the sampling chamber 2 and the third magnet 44 in the cavity 41 are in a mutually repulsive state, so that the third magnet 44 moves downwards under the repulsive force of the second magnet 21, and further generates a downward pressure on the spring 45 fixedly connected between the lower surface of the third magnet 44 and the lower surface of the cavity 41, so that the spring 45 is in a compressed state, at the same time, the hose 43 communicating the sampling chamber 2 and the sampling tube 7 is not extruded by the conical block 42 and the third magnet 44, the hose 43 is in a smooth state, when the sampling handle 33 is pulled upwards, the second magnet 21 moves upwards under the action of the attractive force of the first magnet 32 on the second magnet 21, the distance between the second magnet 21 and the third magnet 44 is increased, the repulsive force between the second magnet 21 and the third magnet 44 is reduced, and the pressure of the third magnet 44 on the spring 45 is reduced, the spring 45 starts to restore the natural state upwards, at the moment, under the action of the upward elastic force of the spring 45 on the magnet III 44, the magnet III 44 moves upwards, after sampling is completed, the magnet III 44 moves upwards to the conical block 42, a channel in the hose 43 is completely closed under the matching of the magnet III 44 and the conical block 42, so that the sampling chamber 2 is in a completely sealed environment, the sample in the sampling chamber 2 after sampling is completed is prevented from being polluted by external sundry bacteria, and meanwhile, the phenomenon that the sample after sampling is extruded out of the sampling chamber 2 under the action of air pressure to cause insufficient sample amount and other appliance pollution after the sample flows out is also avoided.
As an embodiment of the invention, a mounting hole 34 is formed in the piston rod 3, a first sliding groove 31 is formed in the piston rod 3, and the length direction of the first sliding chute 31 is the same as the length direction of the piston rod 3, the number of the first sliding chutes 31 is two, the first sliding grooves 31 are symmetrically distributed on the piston rod 3, the piston rod 3 is provided with second sliding grooves 35, the second sliding grooves 35 are symmetrically distributed at the upper end and the lower end of the first sliding grooves 31, and the joint of the first sliding groove 31 and the second sliding groove 35 is positioned in the middle of the second sliding groove 35, the second sliding groove 35 is communicated with the first sliding groove 31, the second sliding groove 35 is perpendicular to the first sliding groove 31, the fixing rod 5 is slidably mounted in the mounting hole 34, the upper end of the fixed rod 5 is fixedly provided with a strut 51, the strut 51 passes through the second sliding groove 35 and is fixedly connected to the inner wall of the piston hole 11, and the lower end of the fixed rod 5 is connected with the base 4 through threads;
when the sampling device works, the sampling tube 7 extends into a liquid probiotic product, the sampling handle 33 is rotated, so that the piston rod 3 is driven to rotate, the strut 51 which penetrates through the second chute 35 at the upper end of the first chute 31 and is fixedly connected to the inner wall of the piston hole 11 and the second chute 35 at the upper end of the first chute 31 rotate relatively, the strut 51 rotates relatively to the position which is overlapped with the central line of the first chute 31, then the sampling handle 33 is pulled upwards, the strut enters the first chute 31, the piston rod 3 moves upwards, the first magnet 32 is driven to move upwards, the second magnet 21 and the piston 22 fixedly connected to the lower surface of the second magnet 21 are driven to move upwards together, after sampling is completed, the sampling handle 33 is rotated again, so that the strut 51 rotates relatively to the second chute 35 at the lower end of the first chute 31, the strut rotates relatively to enter the second chute 35, and the piston rod 3 is fixed, avoid the sample to accomplish the back because of the piston rod 3 not fixed lead to making the sample after the sample under the effect of sampling chamber 2 internal air pressure difference again discharged, simultaneously after the sampling operation is accomplished, because pass through threaded connection between base 4 and the dead lever 5 to make base 4 can dismantle, consequently after the sample is accomplished, dismantle base 4 and carry out thorough washing and sterilization process to the appearance of taking a sample, thereby guarantee that the appearance of taking a sample all is in sterile condition when carrying out sampling operation at every turn.
As an embodiment of the invention, one end of the hose, which is far away from the conical block, is a spiral hose 46, the lower surface of the base 4 is fixedly provided with a rubber layer 6, the lower surface of the rubber layer 6 is fixedly provided with a sampling tube 7, and the sampling tube 7 penetrates through the rubber layer 6 and is fixedly connected with the spiral hose 46;
when in use, because the end of the hose 43 far away from the conical block 42 is the spiral hose 46, the rubber layer 6 is fixedly arranged on the lower surface of the base 4, and the sampling tube is fixed by the extrusion of the rubber layer 6, during sampling operation, a worker can manually pull the sampling tube 7 to move, further adjusting the sampling position and the sampling depth of the sampling tube 7 in the liquid probiotic product, so that the sampling instrument can respectively sample samples at different positions and depths, thereby improving the sampling range, avoiding the problem that sampling operators need to repeatedly sample samples at different positions and depths, improving the sampling efficiency, reducing the possibility of mixed bacteria pollution, and simultaneously, because the sampling operation can be carried out on the samples at different positions and depths, the sampling randomness is improved, and the detection result after sampling is more accurate and effective.
In one embodiment of the present invention, the sampling tube 7 is made of a rigid plastic tube;
the during operation, after sampling operation, because the material of sampling tube 7 is the stereoplasm plastic tubing, consequently sampling tube 7 has elasticity, can take place bending deformation after receiving the extrusion, thereby make after the sample is accomplished, through upwards extruding sampling tube 7, thereby make it upwards take place bending deformation and accomodate in cavity 41, use aseptic protective sheath simultaneously with the still exposed position cover outside of sampling tube 7, the direct exposed position outside of sampling tube 7 has been reduced, avoided sampling tube 7 directly to lead to sampling tube 7 to be polluted by miscellaneous fungus with external contact after the sample is accomplished, thereby cause the pollution to next sample, simultaneously also avoided sampling tube 7 directly to expose outside mistake injury operating personnel.
A probiotic sampling method is suitable for the probiotic sampling instrument, and comprises the following steps:
s1, inserting the sampling tube 7 at the lower end of the sampler into the sample to be sampled, and adjusting the position of the sampling tube 7;
s2, rotating the sampling handle 33, rotating the piston rod 3 to rotate the support 51 to the center of the first sliding groove 31, pulling the sampling handle 33 to drive the piston rod 3 and the first magnet 32 on the piston rod 3 to move upwards, so as to drive the second magnet 21 and the piston 22 to move upwards together, and pumping the sample into the sampling chamber 2;
s3, after sampling is completed, the repulsive force between the second magnet 21 and the third magnet 44 is reduced, the spring 45 returns to the natural state upwards to drive the third magnet 44 to move upwards, and the hose 43 is extruded together with the conical block 42 to seal the sampling chamber 2;
s4, rotating the sampling handle 33 to enable the pillar 51 to be positioned in the second sliding groove 32, fixing the piston rod 3 to complete the sampling process, and then taking out the sample in the sampling chamber 2 for detection or storage operation;
s5, after the operation of S3 is completed, the base 4 is taken down, and the sampling instrument is cleaned and sterilized to be in an aseptic state when being used next time;
s6, after cleaning and sterilization, the sampling tube 7 is extruded upwards, the sampling tube 7 is stored in the cavity 41, then the base 4 is covered with the sterile protective sleeve, and the sampler 7 is stored in a dry and sterile environment for the next use.
The specific working process is as follows:
the sampling tube 7 is inserted into the liquid probiotic product, the position and the depth of the sampling tube 7 are adjusted, the sampling handle 33 is rotated, the support 51 is rotated to the center of the first sliding groove 31 from the second upper sliding groove 35, the sampling handle 33 is pulled upwards to drive the piston rod 3 to slide upwards, at the moment, the first magnet 32 is fixedly arranged on the outer surface of the piston rod 3, the second magnet 21 is slidably arranged in the sampling chamber 2, and the piston 22 is fixedly arranged on the lower surface of the second magnet 21, therefore, when the piston rod 3 slides upwards, the first magnet 32 simultaneously moves upwards, because the first magnet 32 and the second magnet 21 attract each other, the second magnet 21 and the piston 22 also slide upwards together, because the third magnet 44 and the second magnet 21 in the cavity 41 repel each other, the third magnet 44 pushes the spring 45 downwards under the repulsion force of the second magnet 21, the repulsion force of the third magnet 44 is reduced when the second magnet 21 moves upwards, therefore, the pressure of the spring 45 is also reduced by the magnet III 44, the spring 45 starts to restore the natural state upwards to generate an upward elastic force on the magnet III 44, so that the magnet III 44 moves upwards, meanwhile, the magnet III 44 moves upwards to be matched with the conical block 42 to completely seal a channel between the sampling chamber 2 and the hose 43, so that the sampling chamber 2 is in a sealed state, after sampling is completed, the sampling handle 33 is rotated to rotate the support 51 to the lower chute II 35 away from the center of the chute I31, the base 4 is rotated to disassemble the base 4 for complete cleaning and sterilization treatment, then the sampling tube 7 is upwards extruded to accommodate the sampling tube 7 into the cavity 41, and the sterile protective sleeve is sleeved at the exposed position of the sampling sleeve 7.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. The utility model provides a probiotic appearance of taking a sample which characterized in that: including sampling sleeve (1), piston hole (11) have been seted up in sampling sleeve (1), the central line coincidence of piston hole (11) central line and sampling sleeve (1), a plurality of sampling rooms (2) have been seted up in sampling sleeve (1), sampling room (2) are along circumferencial direction evenly distributed, each other do not communicate between sampling room (2), slidable mounting has piston rod (3) in piston hole (11), piston rod (3) upper end fixed mounting has sample handle (33), piston rod (3) surface fixed mounting has magnet (32), magnet (32) are in the one side that is close to piston rod (3) lower extreme, slidable mounting has magnet two (21) in sampling room (2), fixed surface mounting has piston (22) under magnet two (21), attract each other between magnet (32) and magnet two (21), fixed surface installs base (4) under sampling sleeve (1), fixed surface installs sampling tube (7) under base (4), one-to-one between sampling tube (7) and sampling room (2), just sampling tube (7) and the inside intercommunication each other of sampling room (2).
2. The probiotic sampling instrument of claim 1, characterized in that: base (4) inside cavity (41) of having seted up, one-to-one between cavity (41) and sampling chamber (2), fixed surface installs conical block (42) on cavity (41), hose (43) are installed in conical block (42) outside, hose (43) link firmly in cavity (41) upper surface department, just communicate each other between hose (43) and the sampling chamber (2), slidable mounting has three (44) of magnet on hose (43) surface, repel each other between three (44) of magnet and two (21), fixed surface is connected with spring (45) under three (44) of magnet, the other end fixed connection of spring (45) is in cavity (41) lower surface department.
3. The probiotic sampling instrument of claim 1, characterized in that: the piston rod (3) is internally provided with a mounting hole (34), the piston rod (3) is provided with a first sliding chute (31), the length direction of the first sliding chute (31) is the same as that of the piston rod (3), the first sliding chute (31) is provided with two sliding chutes, the first sliding chute (31) is symmetrically distributed on the piston rod (3), the piston rod (3) is provided with a second sliding chute (35), the second sliding chute (35) is symmetrically distributed at the upper end and the lower end of the first sliding chute (31), the joint of the first sliding chute (31) and the second sliding chute (35) is positioned at the middle position of the second sliding chute (35), the second sliding chute (35) is communicated with the first sliding chute (31), the second sliding chute (35) is perpendicular to the first sliding chute (31), a fixing rod (5) is slidably mounted in the mounting hole (34), a support column (51) is fixedly mounted at the upper end of the fixing rod (5), the pillar (51) penetrates through the sliding groove (31) and is fixedly connected to the inner wall of the piston hole (11), and the lower end of the fixing rod (5) is connected with the base (4) through threads.
4. The probiotic sampling instrument of claim 1, characterized in that: one end, far away from the conical block (42), of the hose (43) is a spiral hose (46), the rubber layer (6) is fixedly mounted on the lower surface of the bottom, the sampling tube (7) is fixedly mounted on the lower surface of the rubber layer (6), and the sampling tube (7) penetrates through the rubber layer (6) to be fixedly connected with the spiral hose (46).
5. The probiotic sampling instrument of claim 1, characterized in that: the sampling tube (7) is made of a hard plastic tube.
6. A sampling method of probiotics is characterized in that: the sampling method is suitable for the probiotic bacteria sampling instrument in the claims 1-5, and comprises the following steps:
s1, inserting a sampling tube (7) at the lower end of the sampler into a sample to be sampled, and adjusting the position of the sampling tube (7);
s2, rotating the sampling handle (33), rotating the piston rod (3) to rotate the support column (51) to the center of the first sliding groove (31), pulling the sampling handle (33) to drive the piston rod (3) and the first magnet (32) on the piston rod (3) to move upwards, so as to drive the second magnet (21) and the piston (22) to move upwards together, and pumping the sample into the sampling chamber (2);
s3, after sampling is completed, the repulsive force between the second magnet (21) and the third magnet (44) is reduced, the spring (45) returns to the natural state upwards to drive the third magnet (44) to move upwards, and the hose (43) is extruded together with the conical block to seal the sampling chamber (2);
s4, rotating the sampling handle (33), enabling the support column (51) to be located in the second sliding groove (32), fixing the piston rod (3), completing a sampling process, and then taking out a sample in the sampling chamber (2) for detection or storage operation;
s5, after the operation of S3 is completed, the base (4) is taken down, and the sampling instrument is cleaned and sterilized to be in an aseptic state when being used next time;
s6, after cleaning and sterilization, the sampling tube is upwards extruded, the sampling tube is stored in the cavity, the bottom end (4) is covered with the sterile protective sleeve, and the sampler (7) is stored in a dry and sterile environment to wait for next use.
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Cited By (1)
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| CN115824719A (en) * | 2023-02-14 | 2023-03-21 | 河北会有环保科技有限公司 | Harmful substance filtering and sampling device for water quality detection |
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