CN113854351A - Application of nano silicon in fresh-keeping agent for ginger - Google Patents
Application of nano silicon in fresh-keeping agent for ginger Download PDFInfo
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- CN113854351A CN113854351A CN202111026596.9A CN202111026596A CN113854351A CN 113854351 A CN113854351 A CN 113854351A CN 202111026596 A CN202111026596 A CN 202111026596A CN 113854351 A CN113854351 A CN 113854351A
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- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Storage Of Fruits Or Vegetables (AREA)
Abstract
The invention relates to the technical field of food preservation, and discloses application of nano-silicon in a ginger preservative, wherein the optimal preservation concentration of a nano-silicon solution is 100 mg/L. The optimal fresh-keeping concentration of the nano-silicon solution is 100mg/L, and the ginger stored under the concentration can effectively inhibit the rotting and water loss of the ginger and reduce the reduction range of hardness and the change of color difference; the invention discovers that the nano silicon can prolong the preservation period and shelf life of the ginger and provides a new idea for fresh preservation of the ginger.
Description
Technical Field
The invention relates to the technical field of food preservation, in particular to application of nano silicon in a ginger preservative.
Background
Ginger is used as a seasoning vegetable necessary for families, is cultivated in most areas of China, and has the functions of resisting aging and inhibiting tumors when being eaten. However, ginger is not easy to store, and often becomes shriveled, germinant, rotten and deteriorated, and once the edible quality of ginger is reduced, the market sale and price of ginger are affected. The ginger is almost indispensable seasoning on a dining table and is almost necessary for people all the year round, so that the storage time of the ginger must be prolonged, the storage effect is improved, and the ginger is guaranteed to be fresh and tender and delicious after being stored for a long time as far as possible.
Because how to effectively carry out post-harvest treatment and storage on the ginger becomes a bottleneck restricting further development of the ginger industry, in order to solve the problem of storage of the ginger, the ginger can be uniformly listed, and the ginger storage method has important significance for enriching dining tables of people, promoting development of local economy and increasing income of farmers. Therefore, the inventor researches the effect of the nano silicon in fresh keeping of the ginger.
Disclosure of Invention
Based on the problems, the invention provides the application of the nano silicon in the ginger preservative, and the invention discovers that the nano silicon can prolong the preservation period and shelf life of the ginger and provides a new idea for the preservation of the ginger.
In order to solve the technical problems, the invention provides an application of nano silicon in a fresh keeping agent for ginger, and the fresh keeping method comprises the following steps: washing fresh ginger blocks with running water, naturally drying, soaking the ginger blocks in 2% sodium hypochlorite solution for 5min, washing with sterile water for 3 times, sterilizing, filtering with filter paper to absorb water on the surface of the ginger blocks, soaking the ginger blocks in 100mg/L nano-silicon solution for 10min, and storing in a storage cabinet with relative humidity of 95% and storage temperature of 12 deg.C.
Compared with the prior art, the invention has the beneficial effects that: the optimal fresh-keeping concentration of the nano-silicon solution is 100mg/L, and the ginger stored under the concentration can effectively inhibit the rotting and water loss of the ginger and reduce the reduction range of hardness and the change of color difference; the invention discovers that the nano silicon can prolong the preservation period and shelf life of the ginger and provides a new idea for fresh preservation of the ginger.
Drawings
FIG. 1 is a graph showing the results of ginger phenotype and DAB staining and NBT staining in accordance with an embodiment of the present invention;
FIG. 2 is a graph showing hardness comparison of ginger processed at different silicon concentrations according to an embodiment of the present invention;
FIG. 3 is a graph showing the comparison of water loss of ginger processed at different silicon concentrations in accordance with an embodiment of the present invention;
FIG. 4 is a graph showing the comparison of the color difference variation of ginger processed with different silicon concentrations according to the embodiment of the present invention;
FIG. 5 is a graph showing the results of the change of POD \ SOD \ CAT of ginger processed at 100mg/L Si concentration in accordance with the present invention;
FIG. 6 is a graph showing the results of the change in ginger MDA under the treatment of the silicon concentration of 100mg/L in the example of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.
Example (b):
the inventor purchases the Shandong ginger in farmer market, selects healthy and full fresh ginger seeds without diseases, insect pests and mechanical damage, transports the fresh ginger seeds to a laboratory on the same day, and stores the ginger seeds for later use at 4 ℃. The selected nano silicon is as follows: purchased from Sigma-Aldrich (Lot 637238) and having a particle size of 10-20 nm. Directly dissolving the nano-silicon in water, and performing ultrasonic oscillation for 40min to obtain a nano-silicon solution, wherein three concentrations of the nano-silicon solution are configured in this embodiment: 50mg/L, 100mg/L and 150 mg/L.
The experiment was set up with a total of four treatments: 1. ginger piece + H2O (CK); 2. ginger blocks and nano silicon (50 mg/L); 3. ginger blocks and nano silicon (100 mg/L); 4. ginger blocks and nano silicon (150 mg/L).
Dividing ginger into four groups, soaking in the four treated solutions respectively, taking the upper part and the middle part of the stem and the base part of the stem of the sample of each treatment group after soaking for 7d, 14d, 21d and 28d, removing the skin and only keeping the central part, cutting the tissue into pieces with the side length of about 2mm, uniformly mixing, wrapping with tinfoil, quickly freezing with liquid nitrogen, storing in a-80 ℃ ultra-low temperature refrigerator for standby, and repeating the treatment and sampling processes for 3 times.
Then, DAB dyeing is carried out, specifically as follows: taking ginger blocks to be dyed, cutting uniform round slices with the thickness of 2mm on the upper part, the middle part and the base part of the stem block respectively, putting about five slices in each beaker, separating by tinfoil, and simultaneously adding a proper amount of DAB dye liquor until the slices are completely immersed; vacuumizing by using a vacuum pump, and vacuumizing for about 15min at the maximum pressure; covering the beaker with tinfoil paper, shading for 4h, and culturing until hydrogen peroxide in the ginger blocks reacts with DAB to obtain brown blocks. The DAB dye solution preparation method comprises the following steps: 50mg of DAB powder (-20 ℃ storage), and 45ml of sterilized ddH were added to a beaker2O, obtaining DAB initial dye liquor with the final concentration of 1 mg/mL; adding 0.2M hydrochloric acid to adjust the pH value to 3.0 or 3.8 (DAB is easy to dissolve at the pH value), adding a magnetic stirrer oscillator, and stirring to dissolve; 25ul (0.05%) Tween 20 and 2.5ml 200mmNa were added2HPO4And the pH is raised, and the DAB can be used after being completely dissolved. DAB is a light sensitive reagent, the preparation process needs to be covered by tinfoil paper, the DAB dye solution is prepared as it is used, and the use time limit of each time does not exceed one day. The formula of the destaining solution is as follows: ethanol: acetic acid: glycerol is 3:1: 1.
NBT staining was performed: taking ginger blocks to be dyed, cutting uniform round slices (separated by tinfoil paper) with the thickness of 2mm at the upper part, the middle part and the base part of each stem block, directly immersing the slices into a test tube or a beaker filled with NBT solution, vacuumizing by using a vacuum pump, and vacuumizing at the maximum pressure for 10-15 minutes; taking out the slice, placing into different culture dishes for different treatments, developing, pouring staining solution, irradiating in incubator until dark blue spot appears (generally irradiating for about 4 hr), photographing, and taking pictures of NBT and O2-The reaction appeared as blue spots. NBT was formulated as follows: an appropriate amount of NBT dry powder was weighed and dissolved in 10mM phosphate buffer (pH 7.6) to a final concentration of 1mg/mL or 0.5mg/mL NBT solution, and the amount of NBT solution used was prepared as it is without adjusting pH, and stored at 4 ℃ in the dark. Preparation of phosphate buffer: 10mm K2HPO4And 10mm KH2PO4Mix until pH 7.6. Preparing a decoloring solution: ethanol: acetic acid: glycerol is 3:1: 1.
The results after DAB dyeing and NBT dyeing are shown in figure 1, wherein group A in figure 1 is that dyed ginger is adopted, CK and Shandong ginger soaked with 50mg/L, 100mg/L and 150mg/L nano silicon solution are sequentially adopted from left to right, the ginger is transversely cut, and then DAB dyeing and NBT dyeing are respectively adopted; FIG. 1B is a cross-section stained with NBT, which shows that the blue spots of the cross-section are the least, i.e., the content of superoxide anions is the least, after treatment with 100mg/L nano-silicon solution; FIG. 1C shows that 100mg/L of cross-section stained with DAB showed the least brown spots, i.e., the least hydrogen peroxide content.
The inventors compared the hardness, water dispersion and color difference change of ginger processed with different silicon concentrations, and the results are shown in fig. 2, fig. 3 and fig. 4 respectively, and the results show that the hardness of ginger processed with 100mg/L nano silicon solution is better than that of other processed ginger, the water loss is less than that of other processed ginger (i.e. the water retention is better), and the color difference change is minimal (i.e. the color change before and after storage is small).
The change conditions of ginger POD \ SOD \ CAT \ PPO and ginger MDA under the treatment of 100mg/L silicon concentration are respectively shown in the attached drawings 5 and 6, and the results show that the activity of ginger PPO under the treatment of 100mg/L silicon concentration is lower than that of CK treatment, polyphenol oxidase (PPO) can catalyze a plurality of simple phenolic substances to be oxidized to form quinone compounds, and further polymerize to form brown, brown or black polymers, which indicates that nano silicon has a certain inhibition effect on the generation of PPO, namely has a certain antagonism on the generation of browning. The SOD activity of ginger under the treatment of 100mg/L silicon concentration is higher than that of CK, superoxide dismutase (SOD) is an important enzyme in active oxygen metabolism, can effectively remove superoxide radical, and is used for preventing active oxygen or other peroxide radicals from damaging a cell membrane system, so that the toxicity of the radicals to organisms is reduced, and the preservation of ginger under the treatment of 100mg/L silicon concentration is better than that of ginger without the treatment. The CAT activity of the ginger is higher than that of CK under the treatment of 100mg/L silicon concentration, Catalase (CAT) belongs to hemoglobin protease and can catalyze hydrogen peroxide accumulated in a body to be decomposed into water and molecular oxygen, so that the possible oxidative damage of the hydrogen peroxide to fruit and vegetable tissues is reduced, and the higher CAT activity can still reduce the oxidative damage of the hydrogen peroxide to the ginger after the ginger is treated by the 100mg/L silicon concentration, and the ginger is easier to store. The MDA activity of the ginger under the treatment of the silicon concentration of 100mg/L is lower than that of the ginger under the treatment of CK, the MDA is a main product of lipid peroxidation, can react with various components in cells to promote the inactivation of enzymes in the cells, can destroy the structure and the function of a biological membrane, and accelerate the softening of fruits, is considered as an important index of plant senescence and strong and weak reaction to adverse conditions, can reflect the loss degree of the cell membrane and the strength and weakness of adverse damage, and the low MDA activity of the ginger under the treatment of the silicon concentration of 100mg/L indicates that the silicon concentration treatment of 100mg/L has certain effect on the storage of the ginger. The POD of ginger treated by 100mg/L silicon concentration is lower than that of POD treated by CK, peroxidase POD can convert some carbohydrates contained in tissues into lignin, lignification degree is increased, and the activity of peroxidase in rice roots of premature senility and reduction yield is found to be increased, so that peroxidase can be used as a physiological index of tissue aging, and the low activity of ginger POD treated by 100mg/L silicon concentration indicates that the treatment by 100mg/L silicon concentration has a certain function on ginger aging resistance.
Therefore, the method for finally determining the fresh ginger with the nano silicon solution in the embodiment is as follows: washing fresh ginger blocks with running water, naturally drying, soaking the ginger blocks in 2% sodium hypochlorite solution for 5min, washing with sterile water for 3 times, sterilizing, filtering with filter paper to absorb water on the surface of the ginger blocks, soaking the ginger blocks in 100mg/L nano-silicon solution for 10min, and storing in a storage cabinet with relative humidity of 95% and storage temperature of 12 deg.C.
The above is an embodiment of the present invention. The embodiments and specific parameters in the embodiments are only for the purpose of clearly illustrating the verification process of the invention and are not intended to limit the scope of the invention, which is defined by the claims, and all equivalent structural changes made by using the contents of the specification and the drawings of the present invention should be covered by the scope of the present invention.
Claims (1)
1. The application of the nano silicon in the fresh keeping agent for ginger is characterized in that the fresh keeping method comprises the following steps: washing fresh ginger blocks with running water, naturally drying, soaking the ginger blocks in 2% sodium hypochlorite solution for 5min, washing with sterile water for 3 times, sterilizing, filtering with filter paper to absorb water on the surface of the ginger blocks, soaking the ginger blocks in 100mg/L nano-silicon solution for 10min, and storing in a storage cabinet with relative humidity of 95% and storage temperature of 12 deg.C.
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| CN202111026596.9A CN113854351A (en) | 2021-09-02 | 2021-09-02 | Application of nano silicon in fresh-keeping agent for ginger |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101473870A (en) * | 2009-01-16 | 2009-07-08 | 山东农业大学 | Film-coating fresh-keeping technique fresh cut ginger |
| CN102450602A (en) * | 2010-10-21 | 2012-05-16 | 江苏嘉安食品有限公司 | Novel preparation method of ginger |
| CN104170947A (en) * | 2013-07-05 | 2014-12-03 | 四川农业大学 | Composite preserving technology of young ginger and implementation method thereof |
| CN109006983A (en) * | 2018-07-04 | 2018-12-18 | 山东农业大学 | A kind of fresh-cut fruit and vegetable quality control technology |
-
2021
- 2021-09-02 CN CN202111026596.9A patent/CN113854351A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101473870A (en) * | 2009-01-16 | 2009-07-08 | 山东农业大学 | Film-coating fresh-keeping technique fresh cut ginger |
| CN102450602A (en) * | 2010-10-21 | 2012-05-16 | 江苏嘉安食品有限公司 | Novel preparation method of ginger |
| CN104170947A (en) * | 2013-07-05 | 2014-12-03 | 四川农业大学 | Composite preserving technology of young ginger and implementation method thereof |
| CN109006983A (en) * | 2018-07-04 | 2018-12-18 | 山东农业大学 | A kind of fresh-cut fruit and vegetable quality control technology |
Non-Patent Citations (1)
| Title |
|---|
| 蔡德龙 等: "《中国硅肥》", 湖北科学技术出版社, pages: 2 - 3 * |
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Inventor after: Zhu Yongxing Inventor after: Xi Keyong Inventor after: Li Gang Inventor after: Zhu Xiongmeng Inventor after: Tian Ye Inventor after: Liu Deqi Inventor after: Liu Yiqing Inventor before: Xi Keyong Inventor before: Zhu Xiongmeng Inventor before: Tian Ye Inventor before: Li Gang Inventor before: Liu Deqi Inventor before: Zhu Yongxing Inventor before: Liu Yiqing |
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Application publication date: 20211231 |
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