CN113848329A - Biochemical project detection device - Google Patents
Biochemical project detection device Download PDFInfo
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- CN113848329A CN113848329A CN202111257175.7A CN202111257175A CN113848329A CN 113848329 A CN113848329 A CN 113848329A CN 202111257175 A CN202111257175 A CN 202111257175A CN 113848329 A CN113848329 A CN 113848329A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6827—Total protein determination, e.g. albumin in urine
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/728—Bilirubin; including biliverdin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/76—Assays involving albumins other than in routine use for blocking surfaces or for anchoring haptens during immunisation
- G01N2333/765—Serum albumin, e.g. HSA
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91045—Acyltransferases (2.3)
- G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
- G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91188—Transferases (2.) transferring nitrogenous groups (2.6)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
Abstract
The invention discloses a biochemical project detection device. The biochemical item detection device comprises: the device comprises a disc, a plasma quantifying tank, a centrifugal bag placing tank, a diluent quantifying tank, a mixing tank and a mixed liquid transition tank. The biochemical item detection device further comprises: the biochemical item detection holes are provided with a plurality of biochemical detection holes which are uniformly arranged on the edge of the disc, are connected with the mixed liquid transition groove through the liquid inlet flow passage of the detection holes, and meanwhile, freeze-drying reagents for detecting different biochemical items are pre-buried in the biochemical item detection holes. Through carrying out centrifugal operation to the disk, make plasma sample and diluent mix and evenly distributed in a plurality of biochemical project detection hole, according to the difference that detects biochemical project, make mixed liquid and the freeze-drying reagent that corresponds react, and then carry out biochemical project and detect, this detection operation step is simple, convenient, can reach and detect a plurality of blood conventionalities and biochemical detection project's technological effect to single sample simultaneously.
Description
Technical Field
The invention relates to the field of in-vitro diagnosis, in particular to a biochemical item detection device.
Background
The detection of biochemical indicators in plasma using medical devices is the most common technique used in medical diagnostics. In the medical diagnosis in the prior art, the existing blood cell detection and biochemical detection are generally carried out on a blood analyzer and a biochemical analyzer respectively, so that two blood samples and two different instruments are required to be prepared during detection, the detection process is complicated, the sample coding information is easy to generate errors in manual transmission, the time for a patient to wait for a diagnosis result is too long, and the requirement for rapidly detecting different indexes in a blood sample cannot be met. The existing instrument capable of performing blood routine and biochemical detection has the advantages of single detection item, complex structure and complex flow.
Therefore, the medical diagnosis equipment in the prior art has the technical problems of single routine and biochemical detection items of blood and complicated detection operation steps.
Disclosure of Invention
In view of the above, the main objective of the present invention is to provide a biochemical detection device capable of detecting multiple routine and biochemical detection items of blood simultaneously with simple and convenient detection operation steps for a single sample.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
the biochemical item detection device comprises: the device comprises a disc, a plasma quantifying tank, a centrifugal bag placing tank, a diluent quantifying tank, a mixing tank and a mixed liquid transition tank.
Plasma ration groove, centrifugal bag standing groove, diluent ration groove, mixing tank and mixed liquid cross the aqueduct and all set up on the disc, centrifugal bag standing groove with diluent ration groove passes through the diluent feed liquor runner and connects, plasma ration groove with the mixing tank is connected through first siphon runner, diluent ration groove with the mixing tank passes through the second siphon runner and connects, mixing tank and mixed liquid pass through the aqueduct and connect through third siphon runner.
One end of the diluent quantifying groove, which is far away from the second siphon flow channel, is connected with a diluent extending groove, the diluent extending groove is connected with a diluent transition groove through the flow channel, and the diluent transition groove is connected with a plurality of water blank reference holes.
The biochemical item detection device further comprises: the biochemical detection holes are uniformly arranged on the disc and are connected with the mixed liquid transition groove through a liquid inlet flow passage of the detection holes.
Freeze-drying reagents for biochemical detection of different projects are pre-buried in the biochemical project detection holes.
In a preferred embodiment, the biochemical item detecting device further comprises: and the sampling hole is formed in the disc and is connected with the plasma quantifying groove through a flow channel.
In a preferred embodiment, the biochemical item detecting device further comprises: quality control inspection hole, quality control inspection hole set up on the dish piece, quality control inspection hole includes: the first quality control detection hole, the second quality control detection hole and the third quality control detection hole.
The first quality control detection hole is connected with the plasma quantification tank through a flow channel, the second quality control detection hole is connected with the diluent transition tank through a flow channel, and the third quality control detection hole is connected with the mixed liquid transition tank through a flow channel.
In a preferred embodiment, the second quality control detection hole is formed at one end far away from the feed of the diluent, and the third quality control detection hole is formed at one end far away from the feed of the mixed liquid.
In a preferred embodiment, the centrifugal bag is placed in the centrifugal bag placing groove, and the aluminum foil strip above the centrifugal bag is torn before biochemical item detection.
In a preferred embodiment, the width of the first siphon flow passage, the width of the second siphon flow passage and the width of the third siphon flow passage are 0.1-0.5mm, and the depth of the first siphon flow passage, the second siphon flow passage and the third siphon flow passage are 0.1-0.5 mm; the width of the liquid inlet flow channel of the detection hole is 0.5-1.0mm, the depth of the liquid inlet flow channel of the detection hole is 0.05-0.5mm, and the cross sectional area of the liquid inlet flow channel of the detection hole is smaller than that of the third siphon flow channel.
In a preferred embodiment, the surfaces of the first siphon flow channel, the second siphon flow channel and the third siphon flow channel are all subjected to hydrophilic treatment, and the hydrophilicity of the first siphon flow channel and the second siphon flow channel is superior to that of the third siphon flow channel.
In a preferred embodiment, the biochemical item detecting device further comprises: the positioning rubber block placing groove is formed in the edge of the disc, and the positioning prism is fixedly connected to the disc.
Black positioning rubber blocks are placed in the positioning rubber block placing grooves.
In a preferred embodiment, the upper surface of the disc sheet is bonded through a thin film, wherein the thin film can be a PET pressure sensitive film, a PC, PMMA, PS film or the like, and the thickness of the thin film is 0.05-0.2 mm.
In a preferred embodiment, the back surface of the disk is provided with a raised 12-edge structure for fixing the disk and an instrument tray, the height of the 12-edge structure is 1-5mm, and a plurality of circular grooves are distributed on the edge structure and matched with glass beads protruding from the instrument tray for accurately fixing the disk and the instrument.
The biochemical project detection device has the following beneficial effects:
the biochemical item detection device comprises: the device comprises a disc, a plasma quantifying tank, a centrifugal bag placing tank, a diluent quantifying tank, a mixing tank and a mixed liquid transition tank. Make plasma ration groove, centrifugal bag standing groove, diluent ration groove, mixing tank and mixed liquid cross the aqueduct and all set up on the disc, centrifugal bag standing groove passes through the diluent feed liquid runner with diluent ration groove and is connected, and plasma ration groove is connected through first siphon runner with the mixing tank, and diluent ration groove passes through the second siphon runner with the mixing tank and is connected, and the mixing tank passes through the aqueduct with mixed liquid and passes through the third siphon runner and connect.
The biochemical item detection device further comprises: the biochemical item detection holes are provided with a plurality of biochemical detection holes which are uniformly arranged on the edge of the disc, are connected with the mixed liquid transition groove through the liquid inlet flow passage of the detection holes, and meanwhile, freeze-drying reagents for detecting different biochemical items are pre-buried in the biochemical item detection holes.
Through carrying out centrifugal operation to the disk, make plasma sample and diluent mix and evenly distributed in a plurality of biochemical project detection hole, according to the difference that detects biochemical project, make mixed liquid and the freeze-drying reagent that corresponds react, and then carry out biochemical project and detect, this detection operation step is simple, convenient, can reach and detect a plurality of blood conventionalities and biochemical detection project's technological effect to single sample simultaneously.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a front view of a biochemical item detecting apparatus according to an embodiment of the present disclosure;
FIG. 2 is a rear view of a biochemical item detecting apparatus according to an embodiment of the present disclosure;
FIG. 3 is a side view of a biochemical item detecting apparatus according to an embodiment of the present disclosure;
FIG. 4 is a schematic plan view of the biochemical item detecting device after the first centrifugation according to one embodiment of the present disclosure;
FIG. 5 is a schematic plan view of the biochemical item detecting device after the second centrifugation according to an embodiment of the disclosure;
FIG. 6 is a schematic plan view of the biochemical item detecting apparatus after the third centrifugation according to an embodiment of the disclosure.
[ description of main reference symbols ]
1. A disk; 2. a plasma quantification tank; 3. a centrifugal bag placing groove; 4. a diluent quantitative tank; 5, a mixing tank; 6. a mixed liquid transition tank; 7 a diluent liquid inlet flow channel; 8. a first siphon flow path; 9. a second siphon flow channel; 10. a third siphon flow channel; 11. a diluent extension slot; 12. a diluent transition groove; 13. a water blank reference well; 14. biochemical item detection holes; 15. a sample application hole; 16. a quality control monitoring hole; 161. a first quality control detection hole; 162. a second quality control detection hole; 163. a third quality control detection hole; 17. positioning the glue block placing groove; 18. positioning a prism; 19. a 12-sided structure; 20. a circular groove.
Detailed Description
The biochemical item detecting device according to the present invention will be described in detail with reference to the accompanying drawings and embodiments of the present invention.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the application described herein are, for example, capable of operation in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.
As shown in fig. 1 to 6, the biochemical item detecting apparatus includes: the device comprises a disc 1, a plasma quantifying groove 2, a centrifugal bag placing groove 3, a diluent quantifying groove 4, a mixing groove 5 and a mixed liquid transition groove 6. Preferably, the plasma quantifying tank 2 can quantify the plasma sample in a volume of 5 to 30. mu.l, and the diluent quantifying tank 4 can quantify the diluent in a volume of 100 to 500. mu.l.
Make plasma ration groove 2, centrifugal bag standing groove 3, diluent ration groove 4, mixing tank 5 and mixed liquid and cross aqueduct 6 and all set up on disc 1, centrifugal bag standing groove 3 passes through diluent inlet flow channel 7 with diluent ration groove 4 and is connected, and plasma ration groove 2 is connected through first siphon runner 8 with mixing tank 5, and diluent ration groove 4 is connected through second siphon runner 9 with mixing tank 5, and mixing tank 5 and mixed liquid are crossed aqueduct 6 and are connected through third siphon runner 10.
In order to realize the purpose of increasing the accuracy of biochemical project detection by using diluent as a reference, one end of the diluent quantifying groove 4, which is far away from the second siphon flow channel 9, is connected with a diluent extending groove 11, the diluent extending groove 11 is connected with a diluent transition groove 12 through a flow channel, and the diluent transition groove 12 is connected with a plurality of water blank reference holes 13.
Further, the biochemical item detection device further comprises: the biochemical item detection holes 14 are provided with a plurality of biochemical detection holes 14 which are uniformly arranged on the edge of the disc 1 and are connected with the mixed liquid transition groove 6 through a liquid inlet flow passage of the detection holes.
In order to perform a plurality of biochemical item function tests on a single plasma sample, freeze-dried reagents for different biochemical item tests are embedded in the biochemical item test holes 14, and the disk 1 may be classified into a renal function disk, a liver function disk, a preoperative disk, and the like according to the test functions. For example, in the case of 9-item liver function, reagents such as ALB (albumin), ALP (alkaline phosphatase), ALT (alanine aminotransferase), TP (total protein), TBA (total bile acid), AST (aspartate aminotransferase), TC (total cholesterol), TBIL (total bilirubin), and GGT (glutamyltransferase) may be embedded in the biochemical test well 14.
In order to facilitate the injection of the plasma sample, the biochemical item detection device further comprises: and the sample adding hole 15 is formed on the disk 1, is connected with the plasma quantifying groove 2 through the flow channel, and can inject a plasma sample into the plasma quantifying groove through the sample adding hole 15 and the flow channel when the function index of the biochemical project is detected.
Further, the biochemical item detection device further comprises: a quality control inspection hole 16, which makes the quality control inspection hole 16 opened on the disk 1, the quality control inspection hole 16 includes: a first quality control inspection hole 161, a second quality control inspection hole 162, and a third quality control inspection hole 163. The first quality control detection hole 161 is connected to the plasma quantifying tank 2 via a flow channel, the second quality control detection hole 162 is connected to the diluent transition tank 12 via a flow channel, and the third quality control detection hole 163 is connected to the mixed liquid transition tank 6 via a flow channel. Whether the disc centrifugation process is successfully completed each time is judged by observing the liquid states in the first quality control detection hole 161, the second quality control detection hole 162 and the third quality control detection hole 163. If more than one of the first quality control detection hole 161, the second quality control detection hole 162 and the third quality control detection hole 163 has no liquid, the instrument automatically reports an error and prompts a user that there is a problem in the use of the disk.
In order to facilitate the detection of the centrifugal state of the disk by the quality control monitoring hole 16, the second quality control detection hole 162 is formed at one end far away from the liquid inlet of the diluent, and the third quality control detection hole 163 is formed at one end far away from the liquid inlet of the mixed liquid, so that the redundant liquid in the diluent transition tank 12 and the mixed liquid transition tank 6 can respectively enter the second quality control monitoring hole 162 and the third quality control detection hole 163.
Furthermore, a diluent centrifugal bag is placed in the centrifugal bag placing groove 3, an aluminum foil strip above the centrifugal bag is torn off before biochemical item detection is carried out, and due to the fact that dosage of detection items and plasma samples is different, mixing proportion of diluent and the plasma samples is different, and therefore the diluent centrifugal bag with the corresponding dosage can be reasonably selected according to needs.
Preferably, the width of the first siphon flow channel 8, the depth of the second siphon flow channel 9 and the third siphon flow channel 10 is 0.1-0.5mm, the depth of the third siphon flow channel 10 is 0.1-0.5mm, the width of the liquid inlet flow channel of the detection hole is 0.5-1.0mm, the depth of the liquid inlet flow channel of the detection hole is 0.05-0.5mm, and the cross-sectional area of the liquid inlet flow channel of the detection hole is smaller than that of the third siphon flow channel 10.
Furthermore, the surfaces of the first siphon flow channel 8, the second siphon flow channel 9 and the third siphon flow channel 10 are all subjected to hydrophilic treatment, so that liquid can conveniently enter the siphon flow channels when the disc 1 is subjected to a centrifugal process. And the hydrophilicity of the first siphon flow channel 8 and the second siphon flow channel 9 is superior to that of the third siphon flow channel 10, so that the plasma sample and the diluent in the mixing tank 5 can be mixed fully, and the accuracy of biochemical item detection is improved.
Due to the different reaction rates of the mixed liquid sample in the biochemical item detection hole 14 and different freeze-drying reagents, different reference substances are needed for positioning when the signal of the biochemical item detection hole is collected. The biochemical item detection plate further comprises: the positioning rubber block placing groove 17 and the positioning prism 18 are used for enabling the positioning rubber block placing groove 17 to be formed in the edge of the disc 1, the positioning prism 18 is fixedly connected to the disc 1, the black positioning rubber block is placed in the positioning rubber block placing groove 17 and can be used for positioning a detection hole, and the detection hole positioning prism on the outer ring of the disc is also used for positioning the detection hole; the difference between the two positioning schemes is that the disc is positioned by using a black rubber block when signals in the detection hole are collected at a low speed, and is positioned by using a prism when the signals in the detection hole are collected at a high speed.
In order to prevent the liquid in the disk 1 from being thrown out at the time of centrifugation, the upper surface of the disk 1 is bonded by a thin film, which may be a PET pressure sensitive film, a PC, PMMA, PS film, or the like, and has a thickness of 0.05 to 0.2 mm.
In order to facilitate accurate fixation of the disk 1 and an instrument tray, the back surface of the disk 1 is provided with a convex 12-edge structure 19 for fixation of the disk 1 and the instrument tray, the height of the 12-edge structure 19 is 1-5mm, and a plurality of circular grooves 20 are distributed on the 12-edge structure and matched with glass beads protruding on the instrument tray for accurate fixation of the disk 1 and the instrument.
In a specific embodiment, when the biochemical item detection plate is used for detecting different biochemical items of a single plasma sample, the operation steps are as follows:
as shown in FIG. 4, the disk 1 was operated for the first centrifugation at 3000 and 5000rpm, 100. mu.l of whole blood sample was added through the well 15 of the disk 1, and the aluminum foil membrane sealed on the centrifuge capsule was peeled off. After the first centrifugation, the diluent in the centrifugal bag enters the diluent quantifying groove 4 and the water blank reference hole 13 through the diluent liquid inlet flow channel 7, and the redundant diluent enters the second quality control detection hole 162; the whole blood sample enters the plasma quantifying groove 2, the redundant whole blood sample enters the first quality control detection hole 161, and the whole blood sample in the plasma quantifying groove 2 is separated into plasma through centrifugal operation of 60-180s for subsequent use.
As shown in FIG. 5, the disk 1 is operated to perform the second centrifugation at 500-. The diluent in the diluent quantifying groove 4 enters the mixing groove 5 through the first siphon flow channel 8, the plasma sample in the plasma quantifying groove 2 enters the mixing groove 5 through the second siphon flow channel 9, and the plasma and the diluent in the mixing groove 5 are mixed by controlling the disc 1 to accelerate and decelerate quickly.
As shown in FIG. 6, the disk 1 is operated to perform a third centrifugation at 4000-.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention.
Claims (10)
1. A biochemical item detecting device, comprising: the device comprises a disc (1), a plasma quantifying groove (2), a centrifugal bag placing groove (3), a diluent quantifying groove (4), a mixing groove (5) and a mixed liquid transition groove (6);
the plasma quantifying tank (2), the centrifugal bag placing tank (3), the diluent quantifying tank (4), the mixing tank (5) and the mixed liquid transition tank (6) are all arranged on the disc (1), the centrifugal bag placing tank (3) is connected with the diluent quantifying tank (4) through a diluent liquid inlet flow channel (7), the plasma quantifying tank (2) is connected with the mixing tank (5) through a first siphon flow channel (8), the diluent quantifying tank (4) is connected with the mixing tank (5) through a second siphon flow channel (9), and the mixing tank (5) is connected with the mixed liquid transition tank (6) through a third siphon flow channel (10);
one end, far away from the second siphon flow channel (9), of the diluent quantifying groove (4) is connected with a diluent extending groove (11), the diluent extending groove (11) is connected with a diluent transition groove (12) through a flow channel, and the diluent transition groove (12) is connected with a plurality of water blank reference holes (13);
further comprising: a plurality of biochemical item detection holes (14), wherein the biochemical detection holes (14) are uniformly formed in the disc (1) and are connected with the mixed liquid transition groove (6) through a detection hole liquid inlet flow channel;
freeze-drying reagents for biochemical detection of different projects are pre-buried in the biochemical project detection holes (14).
2. A biochemical item detecting device according to claim 1, further comprising: the sampling hole (15), the sampling hole (15) is seted up on the disc (1), through the runner with the plasma ration groove (2) is connected.
3. A biochemical item detecting device according to claim 1, further comprising: a quality control inspection hole (16), the quality control inspection hole (16) being provided on the disk (1), the quality control inspection hole (16) including: a first quality control detection hole (161), a second quality control detection hole (162), and a third quality control detection hole (163);
the first quality control detection hole (161) is connected with the plasma quantification tank (2) through a flow channel, the second quality control detection hole (162) is connected with the diluent transition tank (12) through a flow channel, and the third quality control detection hole (163) is connected with the mixed liquid transition tank (6) through a flow channel.
4. A biochemical item detecting device according to claim 3, wherein the second quality control detecting hole (162) is opened at an end far from the feed of the diluting liquid, and the third quality control detecting hole (163) is opened at an end far from the feed of the mixed liquid.
5. The biochemical item detecting device according to claim 1, wherein the centrifugal bag is placed in the centrifugal bag placing groove (3), and the aluminum foil strip above the centrifugal bag is torn before the biochemical item detection.
6. The biochemical item detecting device according to claim 1, wherein the first siphon flow passage (8), the second siphon flow passage (9) and the third siphon flow passage (10) have a width of 0.1-0.5mm and a depth of 0.1-0.5 mm; the width of the liquid inlet flow channel of the detection hole is 0.5-1.0mm, the depth of the liquid inlet flow channel of the detection hole is 0.05-0.5mm, and the cross sectional area of the liquid inlet flow channel of the detection hole is smaller than that of the third siphon flow channel (10).
7. The biochemical item detecting device according to claim 1, wherein the surfaces of the first siphon channel (8), the second siphon channel (9) and the third siphon channel (10) are all treated with hydrophilic treatment, and the hydrophilicity of the first siphon channel (8) and the second siphon channel (9) is better than the hydrophilicity of the third siphon channel (10).
8. A biochemical item detecting device according to claim 1, further comprising: the positioning rubber block placing groove (17) and the positioning prism (18) are arranged at the edge of the disc (1), and the positioning prism (18) is fixedly connected to the disc (1);
black positioning rubber blocks are placed in the positioning rubber block placing grooves (17).
9. A biochemical item detecting apparatus according to claim 1, wherein the upper surface of the disc (1) is bonded by a thin film, the thin film may be a PET pressure sensitive film, PC, PMMA, PS film, etc., and the thickness of the thin film is 0.05-0.2 mm.
10. A biochemical item detecting device according to claim 1, wherein the back of the disc (1) is a raised 12-edge structure (19) for fixing the disc to the instrument tray, the height of the 12-edge structure (19) is 1-5mm, and a plurality of circular grooves (20) are distributed on the 12-edge structure (19) to match with the protruding beads on the instrument tray for precise fixing of the disc (1) to the instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111257175.7A CN113848329A (en) | 2021-10-27 | 2021-10-27 | Biochemical project detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111257175.7A CN113848329A (en) | 2021-10-27 | 2021-10-27 | Biochemical project detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113848329A true CN113848329A (en) | 2021-12-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111257175.7A Pending CN113848329A (en) | 2021-10-27 | 2021-10-27 | Biochemical project detection device |
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| CN (1) | CN113848329A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114345432A (en) * | 2022-02-24 | 2022-04-15 | 含光微纳科技(太仓)有限公司 | Centrifugal disc for liquid quantification |
| WO2023071365A1 (en) * | 2021-10-27 | 2023-05-04 | 含光微纳科技(太仓)有限公司 | Biochemical item detection device |
| WO2023159870A1 (en) * | 2022-02-24 | 2023-08-31 | 含光微纳科技(太仓)有限公司 | Biochemical item testing disc |
-
2021
- 2021-10-27 CN CN202111257175.7A patent/CN113848329A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023071365A1 (en) * | 2021-10-27 | 2023-05-04 | 含光微纳科技(太仓)有限公司 | Biochemical item detection device |
| CN114345432A (en) * | 2022-02-24 | 2022-04-15 | 含光微纳科技(太仓)有限公司 | Centrifugal disc for liquid quantification |
| WO2023159870A1 (en) * | 2022-02-24 | 2023-08-31 | 含光微纳科技(太仓)有限公司 | Biochemical item testing disc |
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