CN113846141A - Rapid physiological toxicity detection method based on emulsion interface energy induced release and application - Google Patents
Rapid physiological toxicity detection method based on emulsion interface energy induced release and application Download PDFInfo
- Publication number
- CN113846141A CN113846141A CN202110844652.3A CN202110844652A CN113846141A CN 113846141 A CN113846141 A CN 113846141A CN 202110844652 A CN202110844652 A CN 202110844652A CN 113846141 A CN113846141 A CN 113846141A
- Authority
- CN
- China
- Prior art keywords
- emulsion
- fluorescent probe
- sample
- physiological toxicity
- rapidly detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/30—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving catalase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The quick physiological toxicity detection method based on the induction release of the edible emulsion interfacial energy comprises the following steps: 1) dissolving emulsifier in ultrapure water, uniformly and slowly adding an edible emulsion sample, shearing by using a high-speed shearing machine, filtering by using an organic membrane after shearing and homogenizing, and maintaining the temperature to be constant in the whole process; 2) preparing an edible emulsion sample containing oxidoreductase according to volume; 3) dissolving a fluorescent probe in ultrapure water, diluting a fluorescent probe aqueous solution, and setting a determination condition; 4) sucking an edible emulsion sample into a centrifugal tube, putting the centrifugal tube into a sample groove, and starting to measure; the diluted fluorescent probe aqueous solution was added to the sample, and measurement data and a kinetic curve were obtained and analyzed. The detection method is rapid, efficient and simple, has low equipment requirement, accurate result, high repeatability and high sensitivity, and can detect the result only by trace; and can induce and destroy the milk system in vitro and release the interface energy thereof, and is safe and convenient.
Description
Technical Field
The invention belongs to the field of food safety detection, and particularly relates to a method for rapidly detecting physiological toxicity based on emulsion interface energy-induced release and application thereof.
Background
Free radicals, also known as reactive oxygen species or radicals, refer to molecules, atoms or groups of atoms with unpaired valence electrons. The biological active peptide has high chemical activity, instability, short existence time, active property and extremely high reactivity, and can react with biological system components to destroy proteins, lipids and polysaccharide nucleic acid, thereby causing cell damage. The biological systems mainly encounter oxygen free radicals, and the active oxygen free radicals in the body have certain functions, such as immunity and signal transduction processes. However, excessive reactive oxygen radicals have destructive actions, resulting in damage to normal cells and tissues of the human body, thereby causing various diseases. Such as heart disease, senile dementia, Parkinson's disease and tumor. Meanwhile, the food has a certain physiological toxicity to human bodies due to the excessively high free radical content.
The edible emulsion is micro-nano emulsion which is prepared by emulsifying edible oil or fat-soluble components into O/W or W/O by a physical or chemical method, most of the micro-nano emulsion is a specific space emulsion structure formed by mechanical mixing (intensive processing such as homogenizing and shearing, and mild mechanical stirring) or ultrasonic mixing, chemical stabilization and the like, and part of energy is stored in the emulsion structure in the processing process of the micro-nano emulsion for keeping the relative stability of the structure. It has been found that when the emulsion structure is disturbed, the stored energy is released, and if the release process is carried out in the human body, it is very likely to cause inflammation of various degrees. Recently, researches show that emulsion systems such as fish oil and the like can cause inflammation of a part of organisms after being eaten, however, when tracing food safety problems, various conventional indexes such as acid value, peroxide value and the like of the prepared emulsion are normal, physiological blockage is not detected, and the operation process also meets the specifications, which brings about a plurality of questions for the safety of emulsion products. The present inventors have for the first time discovered that these safety problems are related to the free radicals that can be transformed at their interface.
In conclusion, in the face of more and more food emulsion products in life, the evaluation and detection method for physiological toxicity of the food emulsion products in the market still needs to be further improved, and a rapid evaluation method for physiological toxicity of in vitro edible emulsion is urgently needed.
Disclosure of Invention
Based on the defects and shortcomings in the prior art, the invention creatively uses catalase to induce and release the interfacial energy of the emulsion and is based on the inflammation problem caused by excessive free radicals in the food emulsion interface, a fluorescence detection technology is utilized, a rapid physiological toxicity detection method based on the induced and released interfacial energy of the emulsion is established, a detection system of the physiological toxicity of the food is supplemented, and the method has important significance for rapid detection and quality control of related products.
In order to achieve the purpose, the invention adopts the technical scheme that:
the method for rapidly detecting the physiological toxicity based on the emulsion interface energy-induced release comprises the following steps:
1) dissolving emulsifier in ultrapure water, uniformly and slowly adding an edible emulsion sample, shearing by using a high-speed shearing machine, filtering by using an organic membrane after shearing and homogenizing, and maintaining the temperature to be constant in the whole process;
2) preparing an edible emulsion sample containing oxidoreductase;
3) dissolving a fluorescent probe in ultrapure water, diluting a fluorescent probe aqueous solution, and setting a determination condition;
4) sucking an edible emulsion sample into a centrifugal tube, putting the centrifugal tube into a sample groove, and starting to measure; the diluted fluorescent probe aqueous solution was added to the sample, and measurement data and a kinetic curve were obtained and analyzed.
Preferably, the amount of the emulsifier in the step 1 is 100 μ L, the amount of ultrapure water is 100mL, the amount of the edible emulsion is 500 μ L, the shearing speed is 3000-30000rpm, the time is 3-15min, and the temperature is 30-50 ℃.
Preferably, in step 3, the fluorescent probe is a lutidine N-oxide fluorescent probe, the amount of the fluorescent probe is 0.1mg, the amount of ultrapure water is 866 μ L, and the dilution ratio is 1: 4.5;
the determination conditions are as follows: setting the preparation time of an AB-2280 illuminance sensor to be 5s, and setting the measurement time to be 120 s;
in step 4, the amount of the edible emulsion sample is 1100 muL, and the amount of the diluted fluorescent probe aqueous solution is 100 muL; the diluted aqueous solution of the fluorescent probe was added 5s after the start of the measurement.
Preferably, the edible emulsion is prepared from one or more of fish oil, lard oil, olive oil, algae oil, linoleic acid and palm oil.
Preferably, the edible emulsion is prepared in the form of one or more of nano-emulsion, liposome, micelle and vesicle.
Preferably, the oxidoreductase is a catalase. Catalase is able to induce the release of interfacial energy of the food emulsion.
Preferably, the concentration of the reductase contained in the edible emulsion prepared in the step 2 is 0-0.5 mu mol/L.
Preferably, the organic film is 0.22 to 0.45. mu.L of the organic film.
Preferably, the emulsifier is one or more of tween, lecithin, span and glyceride.
The invention also provides application of the rapid detection method for physiological toxicity based on the emulsion interface energy-induced release in analysis of the physiological toxicity of food emulsion.
The invention has the beneficial effects that:
1. the invention uses catalase to induce and release the interfacial energy of the emulsion, and establishes a quick physiological toxicity detection method based on the induced release of the interfacial energy of the emulsion by utilizing a fluorescence detection technology based on the inflammation problem caused by excessive free radicals in the food emulsion interface. The detection method is rapid, efficient and simple, and has low equipment requirement.
2. The detection method has the advantages of accurate result, high repeatability and high sensitivity, and the result can be detected only by trace amount.
3. The detection method can induce and destroy the milk system in vitro and release the interface energy of the milk system, and is safe and convenient.
Drawings
FIG. 1 shows the background ROS content of fish oil emulsions prepared with different emulsifiers and their ROS content after mixing with Catalase (CAT); (Black part: no catalase was added; gray part: catalase was added)
FIG. 2 shows the effect of different Catalase (CAT) concentrations on ROS content in fish oil emulsions (F0 is the background ROS content of fish oil emulsions, F1 is the ROS content of fish oil emulsions after hydrogen peroxide addition);
FIG. 3 effect of Catalase (CAT) addition at different concentrations on ROS content of lard emulsion (days 1, 3);
FIG. 4 effect of Catalase (CAT) addition at different concentrations on ROS content of linoleic acid emulsion;
FIG. 5 effect of different concentrations of Catalase (CAT) addition on the ROS content of DHA algal oil emulsion;
FIG. 6 is a graph showing the physiological toxicity of fish oil emulsions containing Catalase (CAT) at different concentrations (16.7. mu.g/mL for fish oil, and 0/0.24/0.48. mu. mol/L for catalase)
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the invention without any inventive step, are within the scope of protection of the invention.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs.
Example 1: method for rapidly detecting physiological toxicity of fish oil emulsion
Sucking 100 μ L tween 80 or lecithin, dissolving in 100ml ultrapure water, stirring, dissolving, dripping 500 μ L fish oil sample into the system, homogenizing at 18000rpm for five minutes, filtering with 0.22 μ L organic membrane, and maintaining the whole process at 40 deg.C. Respectively preparing fish oil emulsions with catalase concentration of 0/0.24/0.48 mu mol/L.
0.1mg of the lutidine N-oxide fluorescent probe was dissolved in 866. mu.L of ultrapure water, the lutidine N-oxide aqueous solution was diluted at a ratio of 1:4.5, an AB-2200 illumination sensor was turned on, the set setup time was set to 5s, and the measurement time was set to 120 s. And (3) sucking 1100 mu L of emulsion sample into a centrifuge tube, putting the centrifuge tube into a sample groove, clicking to start measurement, waiting for 5s, adding 100 mu L of diluted lutidine N-oxide fluorescent probe aqueous solution into the sample, clicking a start button, and obtaining and analyzing measurement data and a kinetic curve.
Under the conditions, the effect of different emulsifiers, different fish oil concentrations/presence/absence of catalase on the ROS content of the fish oil emulsion and the toxicity of different catalase concentrations on cells were determined, and the details are shown in the attached figure 1, the attached figure 2 and the attached figure 6.
The results show that different emulsifiers have a greater effect on the free radical content of the finished emulsion product. The important analysis in FIG. 2 shows that the free radical content of the sample without catalase is about 80000, the base number is small, and the change is not large with the increase of the fish oil concentration, while the free radical content of the fish oil emulsion added with catalase is exploded and expanded by more than 6 times, and then the analysis in FIG. 6 shows that the survival rate of the cells is reduced after the increase of the catalase concentration. Therefore, the content of free radicals and cytotoxicity are approximately related under the condition that the concentration of catalase is 0-0.48 mu mol/L, the survival rate of cells is reduced due to a large amount of burst free radicals, and therefore, the fish oil emulsion has physiological toxicity to organisms after being taken. The method has good reproducibility, and the result can be detected only by trace amount.
Example 2: method for rapidly detecting physiological toxicity of linoleic acid emulsion
Sucking 100 μ L Tween 80, dissolving in 100mL ultrapure water, stirring for dissolving, dripping 500 μ L linoleic acid sample into the system uniformly and slowly, shearing with a high-speed shearing machine at 30000rpm for 3 min, filtering the sheared homogeneous sample with 0.22 μ L organic membrane, and maintaining the whole process at 47 deg.C. Preparing linoleic acid emulsion containing catalase concentration of 0 μmol/L, 0.2 μmol/L, 0.4 μmol/L, 0.6 μmol/L, 0.8 μmol/L for use.
0.1mg of the lutidine N-oxide fluorescent probe is dissolved in 866 mu L of ultrapure water, the lutidine N-oxide fluorescent probe aqueous solution is diluted according to the proportion of 1:4.5, the measurement conditions of an AB-2200 illumination sensor control panel are set, the preparation time is set to 5s, and the measurement time is set to 120 s. And (3) sucking 1100 mu L of linoleic acid emulsion sample into a centrifuge tube, placing the centrifuge tube into a sample groove, clicking to start measurement, waiting for 5s, adding 100 mu L of diluted lutidine N-oxide fluorescent probe aqueous solution into the sample, clicking a start button, and recording the number of free radicals and the kinetic curve thereof. Partial results are shown in FIG. 4, the free radicals of the sample are exploded 2-4 times and have low base number with the increase of catalase concentration, and the sample is supposed to have no physiological toxicity to human body after being taken. The method has good reproducibility, and the result can be detected only by trace amount.
Example 3: method for rapidly detecting physiological toxicity of algae oil emulsion
Sucking 100 μ L tween 80, dissolving in 100ml ultrapure water, stirring for dissolving, dripping 500 μ L algae oil sample into the system, shearing for 15min with a high speed shearing machine at 3000rpm, filtering the sheared homogeneous sample with 0.45 μ L organic membrane, and maintaining the whole process at 32 deg.C. Preparing algae oil emulsion containing catalase concentration of 0 μmol/L, 0.2 μmol/L, 0.4 μmol/L, 0.6 μmol/L, 1.0 μmol/L for use.
0.1mg of the lutidine N-oxide fluorescent probe is dissolved in 866 mu L of ultrapure water, the lutidine N-oxide fluorescent probe aqueous solution is diluted according to the proportion of 1:4.5, the measurement conditions of an AB-2200 illumination sensor control panel are set, the preparation time is set to 5s, and the measurement time is set to 120 s. And (3) sucking 1100 mu L of algae oil emulsion into a centrifuge tube (the algae oil emulsion can be diluted when the concentration is too high), putting the centrifuge tube into a sample groove, clicking to start measurement, waiting for 5s, adding 100 mu L of diluted lutidine N-oxide fluorescent probe aqueous solution into the sample, clicking a start button, and recording the content of free radicals and the kinetic curve of the free radicals. Part of the results are shown in FIG. 4, with the increase of catalase concentration, the free radicals of the sample are exploded within 4 times and the base number is not high, and the analysis shows that the sample is basically non-physiological toxicity to human body after being taken. The method has good reproducibility, and the result can be detected only by trace amount.
Example 4: rapid detection method for physiological toxicity of lard emulsion
Sucking 100 μ L Tween 80, dissolving in 100mL ultrapure water, stirring for dissolving, dropping 500 μ L lard sample into the system uniformly and slowly, shearing for 7 min with a high speed shearing machine at 15000rpm, filtering the sheared homogeneous sample with 0.22 μ L organic membrane, and maintaining the whole process at 38 deg.C. Preparing lard emulsion with catalase concentration of 0 μmol/L, 0.1 μmol/L, 0.2 μmol/L, 0.3 μmol/L, 0.4 μmol/L, 0.5 μmol/L for use.
0.1mg of the lutidine N-oxide fluorescent probe was dissolved in 866. mu.L of ultrapure water, the lutidine N-oxide fluorescent probe aqueous solution was diluted at a ratio of 1:4.5, the measurement conditions of the AB-2200 illuminance sensor control panel were set, the preparation time was set to 5s, and the measurement time was set to 120 s. And (3) sucking 1100 mu L of lard emulsion into a centrifuge tube (the lard emulsion can be diluted when the concentration is too high), putting the centrifuge tube into a sample tank, clicking to start measurement, waiting for 5s, adding 100 mu L of diluted lutidine N-oxide fluorescent probe aqueous solution into the sample, clicking a start button, and recording the number of free radicals and the kinetic curve thereof. Partial results are shown in fig. 5, as the concentration of catalase increases, a large amount of free radicals of the sample explodes, the number of the free radicals reaches 10 at most, and the physiological toxicity of the human body after the sample is analyzed. The method has good reproducibility, and the result can be detected only by trace amount.
The above embodiment fully shows that the physiological toxicity condition of the food emulsion can be rapidly obtained through ROS detection, and the ROS content obtained based on the fluorescence detection technology is expected to be used as quality evaluation, regulation and judgment of the physiological toxicity of the food emulsion.
The foregoing has outlined rather broadly the preferred embodiments and principles of the present invention and it will be appreciated that those skilled in the art may devise variations of the present invention that are within the spirit and scope of the appended claims.
Claims (10)
1. The method for rapidly detecting the physiological toxicity based on the emulsion interface energy-induced release is characterized by comprising the following steps of:
1) dissolving emulsifier in ultrapure water, uniformly and slowly adding an edible emulsion sample, shearing by using a high-speed shearing machine, filtering by using an organic membrane after shearing and homogenizing, and maintaining the temperature to be constant in the whole process;
2) preparing an edible emulsion sample containing oxidoreductase according to volume;
3) dissolving a fluorescent probe in ultrapure water, diluting a fluorescent probe aqueous solution, and setting a determination condition;
4) sucking an edible emulsion sample into a centrifugal tube, putting the centrifugal tube into a sample groove, and starting to measure; the diluted fluorescent probe aqueous solution was added to the sample, and measurement data and a kinetic curve were obtained and analyzed.
2. The method for rapidly detecting physiological toxicity based on the emulsion interfacial energy induced release as claimed in claim 1, wherein the amount of the emulsifier in step 1 is 100 μ L, the amount of ultrapure water is 100mL, the amount of the edible emulsion is 500 μ L, the shearing speed is 3000-30000rpm, the time is 3-15min, and the temperature is 30-50 ℃.
3. The method for rapidly detecting physiological toxicity based on the emulsion interfacial energy induced release according to claim 1, wherein in the step 3, the fluorescent probe is a lutidine N-oxide fluorescent probe, the amount of the fluorescent probe is 0.1mg, the amount of ultrapure water is 866 μ L, and the dilution ratio is 1: 4.5;
the determination conditions are as follows: setting the preparation time of an AB-2280 illuminance sensor to be 5s, and setting the measurement time to be 120 s;
in step 4, the amount of the edible emulsion sample is 1100 muL, and the amount of the diluted fluorescent probe aqueous solution is 100 muL; the diluted aqueous solution of the fluorescent probe was added 5s after the start of the measurement.
4. The method for rapidly detecting physiological toxicity based on emulsion interfacial energy induced release according to claim 1, wherein the edible emulsion is prepared from one or more of fish oil, lard oil, olive oil, algae oil, unsaturated fatty acid and palm oil.
5. The method for rapidly detecting physiological toxicity based on emulsion interfacial energy induced release according to claim 1, wherein the edible emulsion is prepared in the form of one or more of micro-nano emulsion, liposome, micelle and vesicle.
6. The method for rapidly detecting physiological toxicity based on the release induced by interfacial energy of emulsion according to claim 1, wherein the oxidoreductase is catalase.
7. The method for rapidly detecting physiological toxicity based on the interfacial energy-induced release of emulsion according to claim 1, wherein the concentration of the reductase contained in the edible emulsion prepared in the step 2 is 0-1.0 μmol/L.
8. The method for rapidly detecting physiological toxicity based on emulsion interfacial energy induced release according to claim 1, wherein the organic membrane is 0.22-0.45 μ L organic membrane.
9. The method for rapidly detecting physiological toxicity based on emulsion interfacial energy induced release according to claim 1, wherein the emulsifier is one or more of tween, lecithin, span and glyceride.
10. Use of a method for rapidly detecting physiological toxicity based on the release induced by interfacial energy in emulsion, which is characterized in that the method for rapidly detecting physiological toxicity based on the release induced by interfacial energy in emulsion according to any one of claims 1 to 9 is used for analyzing the physiological toxicity of food emulsion.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110844652.3A CN113846141A (en) | 2021-07-26 | 2021-07-26 | Rapid physiological toxicity detection method based on emulsion interface energy induced release and application |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110844652.3A CN113846141A (en) | 2021-07-26 | 2021-07-26 | Rapid physiological toxicity detection method based on emulsion interface energy induced release and application |
Publications (1)
Publication Number | Publication Date |
---|---|
CN113846141A true CN113846141A (en) | 2021-12-28 |
Family
ID=78975166
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110844652.3A Pending CN113846141A (en) | 2021-07-26 | 2021-07-26 | Rapid physiological toxicity detection method based on emulsion interface energy induced release and application |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113846141A (en) |
Citations (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4961939A (en) * | 1989-06-02 | 1990-10-09 | Nabisco Brands, Inc. | Deodorized water-in-oil emulsion containing fish oil |
US20010036671A1 (en) * | 2000-03-25 | 2001-11-01 | Nick Gina Lynn | Optical antioxidant sensing process |
US20020182736A1 (en) * | 2001-04-02 | 2002-12-05 | Trustees Of Tufts College | Methods to measure lipid antioxidant activity |
CA2483570A1 (en) * | 2004-10-01 | 2006-04-01 | Luminultra Technologies Ltd. | Reagent system and process for adenosine triphosphate monitoring |
CN1759183A (en) * | 2003-01-17 | 2006-04-12 | 丹尼斯科公司 | Method |
CN101231233A (en) * | 2007-12-18 | 2008-07-30 | 北京科技大学 | Method for controlling release velocity of fluorescent probe molecule in emulsions |
ES2332170A1 (en) * | 2008-07-18 | 2010-01-27 | Universidad Politecnica De Valencia | Binding emulsion, production method thereof and use of same as a conditioning thinning medium and as a binding medium in etching inks and paints |
WO2011149513A1 (en) * | 2010-05-26 | 2011-12-01 | Nestec S.A. | Methods for increasing the production or activity of catalase |
CN102520034A (en) * | 2011-12-16 | 2012-06-27 | 江南大学 | Method for electrochemically measuring content of hydroperoxides in emulsion |
WO2013081496A1 (en) * | 2011-12-01 | 2013-06-06 | Kirsanov Dmitry Olegovich | Apparatus and method for determining the toxicity of liquid media |
CN103196884A (en) * | 2013-04-19 | 2013-07-10 | 河北科技大学 | Method for determining biotoxicity of atrazine by utilizing microcystis aeruginosa |
CN103954732A (en) * | 2014-04-21 | 2014-07-30 | 浙江大学 | Method for measuring continuous phase entering rate of free radicals desorbed from latex particles in emulsion polymerization |
JP2016180686A (en) * | 2015-03-24 | 2016-10-13 | 三菱化学フーズ株式会社 | Method for evaluating fat oxidization in fat emulsion |
CN107022599A (en) * | 2016-02-02 | 2017-08-08 | 常州莱道斯生物医药科技有限公司 | The detection method of catalase activity in a kind of Sensitive Detection few cells and tissue |
CN108020539A (en) * | 2018-01-17 | 2018-05-11 | 南开大学 | The enhancing Raman optical spectrum method that a kind of food security quickly detects |
-
2021
- 2021-07-26 CN CN202110844652.3A patent/CN113846141A/en active Pending
Patent Citations (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4961939A (en) * | 1989-06-02 | 1990-10-09 | Nabisco Brands, Inc. | Deodorized water-in-oil emulsion containing fish oil |
US20010036671A1 (en) * | 2000-03-25 | 2001-11-01 | Nick Gina Lynn | Optical antioxidant sensing process |
US20020182736A1 (en) * | 2001-04-02 | 2002-12-05 | Trustees Of Tufts College | Methods to measure lipid antioxidant activity |
CN1759183A (en) * | 2003-01-17 | 2006-04-12 | 丹尼斯科公司 | Method |
CA2483570A1 (en) * | 2004-10-01 | 2006-04-01 | Luminultra Technologies Ltd. | Reagent system and process for adenosine triphosphate monitoring |
CN101231233A (en) * | 2007-12-18 | 2008-07-30 | 北京科技大学 | Method for controlling release velocity of fluorescent probe molecule in emulsions |
ES2332170A1 (en) * | 2008-07-18 | 2010-01-27 | Universidad Politecnica De Valencia | Binding emulsion, production method thereof and use of same as a conditioning thinning medium and as a binding medium in etching inks and paints |
WO2011149513A1 (en) * | 2010-05-26 | 2011-12-01 | Nestec S.A. | Methods for increasing the production or activity of catalase |
WO2013081496A1 (en) * | 2011-12-01 | 2013-06-06 | Kirsanov Dmitry Olegovich | Apparatus and method for determining the toxicity of liquid media |
CN102520034A (en) * | 2011-12-16 | 2012-06-27 | 江南大学 | Method for electrochemically measuring content of hydroperoxides in emulsion |
CN103196884A (en) * | 2013-04-19 | 2013-07-10 | 河北科技大学 | Method for determining biotoxicity of atrazine by utilizing microcystis aeruginosa |
CN103954732A (en) * | 2014-04-21 | 2014-07-30 | 浙江大学 | Method for measuring continuous phase entering rate of free radicals desorbed from latex particles in emulsion polymerization |
JP2016180686A (en) * | 2015-03-24 | 2016-10-13 | 三菱化学フーズ株式会社 | Method for evaluating fat oxidization in fat emulsion |
CN107022599A (en) * | 2016-02-02 | 2017-08-08 | 常州莱道斯生物医药科技有限公司 | The detection method of catalase activity in a kind of Sensitive Detection few cells and tissue |
CN108020539A (en) * | 2018-01-17 | 2018-05-11 | 南开大学 | The enhancing Raman optical spectrum method that a kind of food security quickly detects |
Non-Patent Citations (6)
Title |
---|
JAE KYOO LEE等: "Spontaneous generation of hydrogen peroxide from aqueous microdroplets", PNAS, vol. 116, no. 39, pages 19294, XP055726280, DOI: 10.1073/pnas.1911883116 * |
农清清;竹内亨;张志勇;何敏;: "活性氧在微囊藻毒素-LR致细胞毒性中的作用", 广西医科大学学报, no. 04, pages 37 - 39 * |
张鑫;王旗;: "细胞中活性氧的荧光探针检测法研究进展", 现代预防医学, no. 22, pages 122 - 124 * |
徐阳: "改性木薯淀粉胶粘剂的制备及性能研究", 中 国 胶 粘, vol. 27, no. 2, pages 1 - 5 * |
王瑛瑶;王璋;罗磊;: "水酶法提花生油中乳状液性质及破乳方法", 农业工程学报, no. 12, pages 267 - 271 * |
马小媛;钱卫平;: "抗氧化能力评价方法", 化学进展, no. 08, pages 159 - 168 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Camkerten et al. | Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange | |
Lo Nostro et al. | Self-assembly and antioxidant properties of octanoyl-6-O-ascorbic acid | |
CN111944738B (en) | Porcine epithelial cell oxidative stress model and establishing method and application thereof | |
Cialoni et al. | Altered venous blood nitric oxide levels at depth and related bubble formation during scuba diving | |
CN113332182B (en) | High-stability vegetable oil liposome and preparation and detection methods thereof | |
CN113846141A (en) | Rapid physiological toxicity detection method based on emulsion interface energy induced release and application | |
EP0423801A1 (en) | Process and apparatus for detecting sensitized leukocyte | |
CN113925157A (en) | Beta-carotene emulsion and preparation method thereof | |
Řehulka et al. | Investigations of physiological and pathological levels of total plasma protein in rainbow trout, Oncorhynchus mykiss (Walbaum) | |
CN106525809B (en) | A method of identifying one of theophylline caffeine theobromine or a variety of chemicals whether are added in relieving cough and asthma class Chinese medicine | |
CN114002213A (en) | Application of Cu/Au/Pt-MOFs and visual test paper thereof in detection of H2O2, Cys or glucose | |
JPS6136185B2 (en) | ||
CN115381775B (en) | Fishskin gelatin emulsion stabilized by luteolin and preparation method and application thereof | |
Skhalyakhov et al. | Phenolic compounds and antioxidant potential of wild-growing plant materials of the North Caucasus region | |
US3256151A (en) | Process for preparing calcitonin | |
CN113017091B (en) | Lipid nanoemulsion with function of eliminating intracellular active oxygen free radicals and preparation method thereof | |
CN109078033A (en) | Saussurea involucrata culture inhibits protein non-enzyme glycosylation to react the application in anti-aging product | |
CN104251857A (en) | Three-dimensional wood fiber detection tool | |
Sazhina et al. | The Effect of the Composition of a Liposomal Nanocomplex on the Antioxidant Activity of Murine Blood Plasma and Lipids of the Liver and Brain | |
RU2627459C2 (en) | Method for determining free radical oxidation in model system | |
WO2018087295A1 (en) | Competition test of an enzyme substrate with internal compensation for enzyme activity | |
CN108195808B (en) | Method for detecting oversulfated chondroitin sulfate impurities in heparin sodium | |
Sezgintürk et al. | A hybrid enzyme biosensor based on sulfite oxidase and glucose oxidase for the analyzing of sulfite and glucose | |
RU54196U1 (en) | ANALYZER FOR DETERMINING THE TOTAL ANTIOXIDANT ACTIVITY OF OBJECTS | |
CN116919842A (en) | Stable and safe phycocyanin nanoemulsion preparation and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |