CN113841793A - 一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法 - Google Patents
一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法 Download PDFInfo
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Abstract
本发明提供一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，涉及农业领域，具体涉及一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法。该一种用白酒糟生产高含量饲料多肽蛋白粉制备方法为：将白酒糟780～800kg、麦麸皮90～95kg、玉米粉48～55kg、菜籽油饼48～55kg、尿素12～18kg、磷酸二氢钾为6～8kg，混合均匀后，边搅拌便加入加入发酵剂2.5～3.5kg，用水稀释的食用碱调节pH为5、水份含量为50％时，进入恒温发酵塔在30～32℃密封发酵8天后，再进行烘干粉碎成为260～300目，得饲料多肽蛋白粉。
Description
技术领域
本发明涉及农业领域。一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法。
背景技术
我国酒糟资源丰富，利用方式多样，如用作饲料原料、微生物发酵生产高附加值产品。但由于其原料特性使得很多运用仅限在实验室研究阶段。主要原因如下：(1)鲜酒糟含水量高，脱水干燥困难，很多都是自然风干，堆放久了容易霉变，并且容易产生霉菌毒素的污染。这给酒糟的进一步加工利用带来很大困难。很难大规模的利用酒糟生产生物饲料。(2)酒糟大量饲喂会引起动物中毒，生产性能降，降低经济效益。并且酒糟在动物饲料中的最大添加量超不过30％，利用率低。(3)酒糟纤维素含量高，蛋白质含量相对较低，这是制约酒糟利用的主要因素。纤维素很难降解，单胃动物很难利用，反当动物也只能利用消化率也不到50％，因此，酒糟只能少量添加于饲料中。这对于我国丰富的酒糟资源来说不异于杯水车薪。因此，要想大规模利用酒糟原料首先需要解决脱水烘干的设备问题。其次，在饲料资源的开发与利用上，必需解决酒糟纤维素降解及提高蛋白质含量的问题。微生物发酵处理能明显改善酒糟品质，提高了酒糟的营养特性及利用率，但还需进一步研巧完善酒糟固态发酵工艺，最大程度利用酒糟资源。目前国内对于白酒糟发酵的研究比较多，但是对于发酵酒糟的研巧还在初级阶段，发酵酒糟的营养物质与豆粕、鱼粉等优质的蛋白原料相比较，粗蛋白含量只有豆粕的55％～75％、鱼粉的40％～45％，而粗纤维含量是豆粕的4～5倍，真蛋白的提升幅度只有10％左右，影响发酵酒糟的利用价值，因此发酵酒糟有待进一步研究。
本发明利用微生物能够将酒糟中的淀粉、糖类等营养物质转化为自身的代谢产物，并同时产生脂肪酸，蛋白酶，纤维素酶等酶类降解酒糟中的纤维素含量，进而得多肽及蛋白质，使其成为物美价廉的绿色饲料添加剂。
发明内容
本发明的目的在于针对我国国情，提出了一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法。既可以添加到青贮饲料中喂养各种动物，又添加到全价精饲料中喂养各种动物，为一种物美价廉的绿色饲料添加剂。
为了实现上述目的，本发明所采用的技术方案为，一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，其原料按重量计为：白酒糟780～800kg、麦麸皮90～95kg、玉米粉48～55kg、菜籽油饼48～55kg、尿素12～18kg、磷酸二氢钾为6～8kg，混合均匀后，边搅拌便加入加入生物霉菌剂2.5～3.5kg；
作为优选，所述的生物霉菌剂添加量为3kg；
进一步，一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，其原料按重量计为：白酒糟780kg、麦麸皮95kg、玉米粉45kg、菜籽油饼55kg、尿素15kg、磷酸二氢钾为7kg、生物霉菌剂3kg；
进一步，所述的生物霉菌剂制备过程为：先将纤维素酶75U/g、木聚糖酶200U/g、3-葡聚糖酶20U/g混合后得酶制剂。再将产朊假丝酵母、干酪乳杆菌和枯草芽孢杆菌以2∶1∶1进行接种后再与以上三种酶制剂混合得生物霉菌剂；
制备如上所述的一种用白酒糟生产高含量饲料多肽蛋白粉，包括以下步骤：
a.先将纤维素酶75U/g、木聚糖酶200U/g、3-葡聚糖酶20U/g混合后得酶制剂；
b.再将产朊假丝酵母、干酪乳杆菌和枯草芽孢杆菌以2∶1∶1进行接种后再与以上三种酶制剂混合得生物霉菌剂；
c.将白酒糟780～800kg、麦麸皮90～95kg、玉米粉48～55kg、菜籽油饼48～55kg、尿素12～18kg、磷酸二氢钾为6～8kg，混合均匀后，边搅拌便加入加入生物霉菌剂2.5～3.5kg，用水稀释的食用碱调节pH为5、水份含量为50％时，进入恒温发酵塔在30～32℃密封发酵8天后，再进行烘干粉碎成为260～300目，得饲料多肽蛋白粉；
本发明具备以下优点：
1.将大量废弃的白酒糟经过二次发酵后变废为宝；
2.与未发酵的白酒糟相比较，干物质基础，真蛋白提高了67.6％，粗纤维、酸性洗涤纤维、中性洗涤纤维、粗脂肪(化)分别降低了42.3％、32.5％、27.8％、22.5％，总钙磷分别提高了18.6％、总氨基酸提高了26.7％，其中赖氨酸、蛋氨酸、苏氨酸、亮氨酸、异亮氨酸和脯氨酸等必需氨基酸分别提高了39.3％、41.3％、68.8％、29.6％、11.4％、9.82％，经济效益极佳；
3.既可以添加到青贮饲料中喂养各种动物，又添加到全价精饲料中喂养各种动物，为一种物美价廉的绿色饲料添加剂；
4.既取得了环保效益、又取得了经济效益，一举两得；
5.制备方法简单合理，产品质量稳定，且易于操作、投入较低。
具体实施方式
下面通过具体的实施例对本发明做进一步的详细描述。
实施例1：一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，其原料按重量份计为：白酒糟780kg、麦麸皮95kg、玉米粉45kg、菜籽油饼55kg、尿素15kg、磷酸二氢钾为7kg、生物霉菌剂3kg。
制备如上所述的一种用白酒糟生产高含量饲料多肽蛋白粉，包括以下步骤：
a.先将纤维素酶75U/g、木聚糖酶200U/g、3-葡聚糖酶20U/g混合后得酶制剂；
b.再将产朊假丝酵母、干酪乳杆菌和枯草芽孢杆菌以2∶1∶1进行接种后再与以上三种酶制剂混合得生物霉菌剂；
c.将白酒糟780kg、麦麸皮95kg、玉米粉45kg、菜籽油饼55kg、尿素15kg、磷酸二氢钾为7kg，混合均匀后，边搅拌便加入加入生物霉菌剂3kg，用水稀释的食用碱调节pH为5、水份含量为50％时，进入恒温发酵塔在30～32℃密封发酵7天后，再进行烘干粉碎成为260～300目，得饲料多肽蛋白粉；
经检测，多肽含量为9％，蛋白质含量为45.5％，经济效益极佳。
实施例2：一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，其原料按重量份计为：白酒糟785kg、麦麸皮90kg、玉米粉50kg、菜籽油饼50kg、尿素15kg、磷酸二氢钾为6kg、生物霉菌剂4kg。
制备如上所述的一种用白酒糟生产高含量饲料多肽蛋白粉，包括以下步骤：
a.先将纤维素酶75U/g、木聚糖酶200U/g、3-葡聚糖酶20U/g混合后得酶制剂；
b.再将产朊假丝酵母、干酪乳杆菌和枯草芽孢杆菌以2∶1∶1进行接种后再与以上三种酶制剂混合得生物霉菌剂；
c.将白酒糟785kg、麦麸皮90kg、玉米粉50kg、菜籽油饼50kg、尿素15kg、磷酸二氢钾为6kg，混合均匀后，边搅拌便加入加入生物霉菌剂4kg，用水稀释的食用碱调节pH为5、水份含量为50％时，进入恒温发酵塔在30～32℃密封发酵8天后，再进行烘干粉碎成为260～300目，得饲料多肽蛋白粉；
经检测，多肽含量为10％，蛋白质含量为47.3％，经济效益极佳。
实施例3：一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，其原料按重量份计为：白酒糟780kg、麦麸皮92kg、玉米粉47kg、菜籽油饼53kg、尿素18kg、磷酸二氢钾为6kg、生物霉菌剂4kg。
制备如上所述的一种用白酒糟生产高含量饲料多肽蛋白粉，包括以下步骤：
a.先将纤维素酶75U/g、木聚糖酶200U/g、3-葡聚糖酶20U/g混合后得酶制剂；
b.再将产朊假丝酵母、干酪乳杆菌和枯草芽孢杆菌以2∶1∶1进行接种后再与以上三种酶制剂混合得生物霉菌剂；
c.将白酒糟780kg、麦麸皮92kg、玉米粉47kg、菜籽油饼53kg、尿素18kg、磷酸二氢钾为6kg，混合均匀后，边搅拌便加入加入生物霉菌剂4kg，用水稀释的食用碱调节pH为5、水份含量为50％时，进入恒温发酵塔在30～32℃密封发酵9天后，再进行烘干粉碎成为260～300目，得饲料多肽蛋白粉；
经检测，多肽含量为10％，蛋白质含量为48.6％，经济效益极佳。
以上所述仅为本发明的优选实施例而已，并不用于限制本发明，对于本领域的技术人员来说，在本发明的精神和原则内可以有各种更改和变化，这些等同的变型或替换等，均包含在本发明的保护范围之内。
Claims (4)
1.一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，其特征在于，将将白酒糟780～800kg、麦麸皮90～95kg、玉米粉48～55kg、菜籽油饼48～55kg、尿素12～18kg、磷酸二氢钾为6～8kg，混合均匀后，边搅拌便加入加入生物霉菌剂2.5～3.5kg，用水稀释的食用碱调节pH为5、水份含量为50％时，进入恒温发酵塔在30～32℃密封发酵8天后，再进行烘干粉碎至260～300目，得饲料多肽蛋白粉。经检测发现，与未发酵的白酒糟相比较，干物质基础，真蛋白提高了67.6％，粗纤维、酸性洗涤纤维、中性洗涤纤维、粗脂肪(化)分别降低了42.3％、32.5％、27.8％、22.5％，总钙磷分别提高了18.6％、总氨基酸提高了26.7％，其中赖氨酸、蛋氨酸、苏氨酸、亮氨酸、异亮氨酸和脯氨酸等必需氨基酸分别提高了39.3％、41.3％、68.8％、29.6％、11.4％、9.82％，经济效益极佳。
2.根据权利要求1所述的一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，其特征在于，其原料按重量计为，白酒糟780～800kg、麦麸皮90～95kg、玉米粉48～55kg、菜籽油饼48～55kg、尿素12～18kg、磷酸二氢钾、发酵剂2.5～3.5kg。
3.根据权利要求要求1或2所述的一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，其特征在于，所述的发酵剂制备过程为：先将纤维素酶75U/g、木聚糖酶200U/g、3-葡聚糖酶20U/g混合后得酶制剂。再将产朊假丝酵母、干酪乳杆菌和枯草芽孢杆菌以2∶1∶1进行接种后再与以上三种酶制剂混合得生物霉菌剂。
4.根据权利要求1或2所述的一种用白酒糟生产高含量饲料多肽蛋白粉的制备方法，所述的纤维素酶、木聚糖酶、3-葡聚糖酶、产朊假丝酵母、干酪乳杆菌和枯草芽孢杆菌均为市售产品。
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