CN113841533A - Pre-harvest application method for improving preservative and fresh-keeping effects of cherry tomatoes - Google Patents

Pre-harvest application method for improving preservative and fresh-keeping effects of cherry tomatoes Download PDF

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CN113841533A
CN113841533A CN202111015226.5A CN202111015226A CN113841533A CN 113841533 A CN113841533 A CN 113841533A CN 202111015226 A CN202111015226 A CN 202111015226A CN 113841533 A CN113841533 A CN 113841533A
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cherry tomatoes
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CN113841533B (en
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弓德强
胡美姣
李敏
高兆银
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CATAS Environment and Plant Protection Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins

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Abstract

The invention discloses a pre-harvest application method for improving the preservative and fresh-keeping effects of cherry tomatoes, which comprises the following steps: (1) before seedling and sowing of cherry tomatoes, soaking dry seeds in water, and then soaking the seeds in a mixed medicament A; the mixed medicament A comprises gamma-aminobutyric acid, salicylic acid and hypersensitive protein; (2) spraying the mixed medicament B to the fruits in the small fruit period and the color transition period of the cherry tomatoes; the mixed medicament B comprises gamma-aminobutyric acid, salicylic acid, compound sodium nitrophenolate and an emulsifier. The cherry tomatoes treated by the method are harvested when the surfaces of the cherries are all red, and are placed into a foam box for storage at normal temperature, so that the fruit rot in the storage period can be effectively reduced, and the cherry tomatoes have good preservative and fresh-keeping effects. The pre-mining technology is convenient to apply, simple to operate, green, safe, efficient, friendly to ecological environment and good in economic benefit and social benefit.

Description

Pre-harvest application method for improving preservative and fresh-keeping effects of cherry tomatoes
Technical Field
The invention relates to the technical field of cherry tomato preservation, in particular to a pre-harvest application method for improving the preservative and fresh-keeping effects of cherry tomatoes.
Background
Cherry tomatoes (also known as cherry tomatoes, small tomatoes, and the like) can be used as vegetables and fruits and are rich in nutrition. Because the appearance is exquisite and lovely, the sugar content is very high, the taste is fragrant, sweet and delicious, and the flavor is unique and is widely loved by consumers. Cherry tomatoes are mainly distributed in China such as Hainan, Guangdong, Guangxi, Yunnan, Sichuan and Fujian. In recent years, the cherry tomato industry has been rapidly developed, the variety is more and more abundant, the yield is continuously increased, but the cherry tomato industry also faces challenges brought by various problems. Firstly, the occurrence of diseases of cherry tomatoes in the field growth process is increasing day by day, the control difficulty is also increased, and some pathogenic bacteria are easy to be latently impregnated on the surfaces of fruits, so that the fruits are extremely easy to rot and mildew in storage and transportation after being picked, and huge economic loss is caused; secondly, the cherry tomatoes belong to respiration jump type fruits, after being picked, the cherry tomatoes are vigorous in respiratory metabolism and not resistant to storage and preservation, and due to the characteristics of thin and juicy skins, the cherry tomatoes are extremely easy to dehydrate and wither without effective preservation measures in the storage and transportation processes, and then mildew and rot occur until the commodity value is completely lost. At present, the research on cherry tomato preservation technology mainly focuses on postharvest treatment, including low-temperature storage, modified atmosphere packaging, biological coating and the like. But the labor cost and the loss are increased by the complicated post-harvest treatment; and improper or misused chemical pesticide application by growers in field production often causes the quality of cherry tomatoes to be reduced, and is not beneficial to food safety and ecological environment protection. Therefore, starting from before picking, the research and development of the before-picking application technology which is safe, efficient, low in cost, simple and convenient to operate and easy to popularize and apply and can improve the cherry tomato preservative and fresh-keeping effects has very important practical significance.
At present, the disease resistance induction technology has become one of the hot spots of the research on the preservation and freshness of picked fruits and vegetables. The resistance inducer has no direct bactericidal effect on pathogens, can induce plants to generate immunocompetence, plays a role in disease resistance and disease prevention, and is widely applied to agricultural production. Salicylic Acid (SA) is an endogenous signal molecule similar to plant hormones, can activate plants to generate systemic acquired resistance, and plays an important role in regulation of physiological processes such as plant maturation and aging and induction of stress-resistant reactions. Researches show that exogenous SA treatment before or after picking can improve the resistance of fruits and vegetables such as mangoes, bananas, tomatoes, pears and the like to diseases after picking, and achieve good effects of corrosion prevention and freshness preservation. Gamma-aminobutyric acid (GABA) is a four-carbon amino acid, widely found in animals, plants and microorganisms, and plays a critical role in plant growth and development and resistance reactions. It has been shown that GABA treatment can improve cold resistance of peach and pumpkin during postharvest cold storage and reduce fruit cold damage. It has also been reported that GABA treatment induces resistance of postharvest pear fruits to penicilliosis, inhibiting decay of sweet cherry fruits. At present, no application research report that salicylic acid and gamma-aminobutyric acid improve the preservative and fresh-keeping effects of picked fruits through seed soaking treatment in the seedling raising period on cherry tomatoes is found.
The hypersensitive protein (Harpin protein, trade name: combretastatin) is a polypeptide compound, is an activator protein which induces the plant to generate anaphylactic reaction (HR) and can stimulate the plant to generate Systemic Acquired Resistance (SAR). The Harpin protein is obtained by fermentation of engineering bacteria containing natural genes, is easy to be identified by plant surface receptors, can activate endogenous signal conduction in plants, and is a broad-spectrum plant function activator. Compound Sodium Nitrophenolate (CSN) also known as Aiduoshou (atonik), Fengyuchu, Tefengshou, the chemical composition is 5-nitroguaiacol sodium, ortho-nitrophenol sodium, para-nitrophenol sodium; after contacting with crops, the fertilizer can quickly permeate into the plant body, promote the protoplasm flow of cells and improve the cell activity; meanwhile, the growth and development can be promoted, flowers and fruits can be prevented from falling, the product quality can be improved, and the yield and the stress resistance of crops can be improved. Research reports that the compound sodium nitrophenolate has the effects of antisepsis and preservation when applied to litchis and mangoes. At present, no application report of the hypersensitive protein and the compound sodium nitrophenolate in the preservation and the preservation of the cherry tomatoes is found. At present, in production, the preservation of cherry tomatoes is usually only paid attention to postharvest, and the link before harvest is easy to be ignored. Gamma-aminobutyric acid, salicylic acid, hypersensitive protein and compound sodium nitrophenolate have no toxic or side effect on human bodies and are friendly to the ecological environment. Therefore, starting from before picking, the substances are applied to seed soaking in the cherry tomato seedling raising period and field resistance inducing treatment in the growth period, and a set of safe and efficient before-picking application technology which is simple and convenient to operate and can effectively improve the cherry tomato preservative and fresh-keeping effects is developed, so that the method is very important.
The present application has been made for the above reasons.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a pre-harvest application method for improving the preservative and fresh-keeping effects of cherry tomatoes. Starting from the pre-picking process, the method is environment-friendly, efficient, convenient and fast to operate and has good application prospect.
The technical scheme of the invention mainly comprises the following contents:
a pre-harvest application method for improving the preservative and fresh-keeping effects of cherry tomatoes comprises the following steps:
(1) before seedling and sowing of cherry tomatoes, soaking dry seeds in water, and then soaking the seeds in a mixed medicament A;
the mixed medicament A comprises gamma-aminobutyric acid, salicylic acid and hypersensitive protein;
(2) spraying the mixed medicament B to the fruits in the small fruit period and the color transition period of the cherry tomatoes;
the mixed medicament B comprises gamma-aminobutyric acid, salicylic acid, compound sodium nitrophenolate and an emulsifier.
Preferably, the mixed medicament A comprises 100-2000 mg/L gamma-aminobutyric acid, 10-100 mg/L salicylic acid and 100-500 mg/L hypersensitivity protein.
Preferably, the mixed medicament B comprises 100-2000 mg/L of gamma-aminobutyric acid, 10-100 mg/L of salicylic acid, 200-1000 mg/L of compound sodium nitrophenolate and an emulsifier with the volume percentage concentration of 0.05-0.2%.
Preferably, the emulsifier is abamectin 4209-A phoxim.
Preferably, the mixed medicament A comprises 1000mg/L gamma-aminobutyric acid, 40mg/L salicylic acid and 500mg/L hypersensitivity protein.
Preferably, the mixed medicament B comprises 1000mg/L gamma-aminobutyric acid, 40mg/L salicylic acid, 500mg/L compound sodium nitrophenolate and 0.1% of emulsifier by volume percentage.
Preferably, the seed soaking time with water is 3-5 h.
Preferably, the seed soaking time of the mixed medicament A is 1-3 h.
Preferably, the small fruit period is 50-60 days after the seedlings are transplanted, and the color conversion period is 100-110 days after the seedlings are transplanted.
The invention has the following effects:
the invention combines seed soaking treatment in the seedling growing period with resistance inducing treatment in the growing period, thereby achieving the purposes of reducing the rottenness of picked cherry tomatoes and improving the fruit preservative and fresh-keeping effects. When the cherry tomato fruits treated by the method are stored for 15 days in a warehouse with the normal temperature of 25 +/-1 ℃ and the relative humidity of 80-90 percent, the fruit rot is obviously reduced, the rot rate is 1.73-5.21 percent and is reduced by 15.89-19.37 percent compared with the control fruit (21.10 percent), and the prevention effect reaches 75.31-91.80 percent. The pre-harvest application method can effectively reduce the rotting rate of cherry tomatoes in normal-temperature storage, improves the anti-corrosion and fresh-keeping effects, and is safe, environment-friendly, efficient, convenient to operate and easy to popularize and apply.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The method for defining the rotten fruits of the cherry tomatoes and calculating the rotten rate in the following examples of the invention comprises the following steps:
investigating the rotten condition of the cherry tomato fruits in the normal-temperature storage period, and counting rotten fruits according to the judgment standard by whether the fruits are wholly or partially mildewed, rotten or juiced.
Rotting rate (%). rotten fruit number × 100/total fruit number investigated.
Control effect (%) (decay rate of control fruit-decay rate of treated fruit) × 100/decay rate of control fruit.
The selection and treatment method of the induced disease-resistant preservative adopted by the invention is made on the basis of the following theory and experimental results:
the research of the inventor shows that the preservative and fresh-keeping effects of the resistance inducing agent SA (40mg/L) and GABA (1000mg/L) in the fruit period before harvest (60 d after seedling transplantation) and the color transition period (100 d after seedling transplantation) for 2 times are better than the effects of spraying the resistance inducing agent SA (40mg/L) and GABA (1000mg/L) for 1 time only in the fruit period (60 d after seedling transplantation) (see table 1); researches show that the rot of cherry tomato fruits can be delayed by spraying SA, GABA, CSN and combination treatment thereof 2 times in the small fruit period (50 d after seedling transplantation) and the color-changing period (100 d after seedling transplantation) in the growth period before harvest, and the combination treatment effect of GABA (1000mg/L), SA (40mg/L) and CSN (500mg/L) is better than that of GABA, SA and CSN (see table 2); the inventor also finds that SA (40mg/L) and GABA (1000mg/L) seed soaking treatment before sowing in the seedling raising period can improve the preservative and fresh-keeping effects in normal-temperature storage after the cherry tomato fruits are picked, the (SA + GABA) composite seed soaking treatment effect is obviously superior to that of SA and GABA single seed soaking treatment, and the (SA + GABA + hypersensitive protein) composite seed soaking treatment effect is superior to that of (SA + GABA) composite seed soaking treatment (see table 3); the inventor further combines the seed soaking treatment A in the seedling growing period with the induced resistance treatment B in the field growing period, and researches show that the treatment method can obviously reduce the rotting rate of the cherry tomato fruits in the normal-temperature storage period and improve the fruit preservative and fresh-keeping effects, and the effects are obviously superior to those of the seed soaking treatment A and the growth period treatment B which are independently treated (see table 4). Therefore, the pre-harvest application method combining seed soaking in the seedling growing period and field induced resistance treatment in the growing period has good preservative and fresh-keeping effects.
TABLE 1 Effect of pre-harvest spray treatment of resistance-inducing agent for different times on preservation and preservation of cherry tomato fruits
Figure BDA0003239721910000041
Note: i and I respectively represent 1 time of each treatment in the small fruit stage (60 d after seedling transplantation) and the color transition stage (100 d after seedling transplantation), and I + I represents 2 times of treatment. The different lower case english letters after the same column of data in the table represent significant differences at the 5% level. The same applies below.
TABLE 2 Effect of Pre-harvest SA, GABA, CSN and combination treatments on preservative and preservative effectiveness of cherry tomato fruits (storage 14d)
Figure BDA0003239721910000051
Note: the treatment is carried out for 1 time respectively in the small fruit period and the color conversion period, namely the treatment is sprayed at 50 days and 100 days after the seedlings are transplanted.
TABLE 3 influence of seed soaking treatment before seedling cultivation and sowing on the preservative and fresh-keeping effect of cherry tomato fruits (storage 15d)
Figure BDA0003239721910000052
Note: the concentrations of SA, GABA and hypersensitive protein were 40mg/L, 1000mg/L and 500mg/L, respectively.
TABLE 4 influence of soaking before seedling cultivation and inducing anti-bonding treatment in growth period on the preservation and fresh-keeping effect of cherry tomato fruit (storage 15d)
Figure BDA0003239721910000053
Note: a comprises gamma-aminobutyric acid, salicylic acid and hypersensitivity protein, B comprises gamma-aminobutyric acid, salicylic acid and compound sodium nitrophenolate; the concentrations of SA, GABA, CSN and hypersensitivity protein are 40mg/L, 1000mg/L, 500mg/L and 500mg/L respectively; treating the field B agent for 2 times in the growing period, namely a small fruit period (50 d after seedling transplantation) and a color conversion period (100 d after seedling transplantation).
Embodiment 1 pre-harvest application method for improving preservative and fresh-keeping effects of cherry tomatoes
(1) Before seedling and sowing of cherry tomatoes, dry seeds are soaked for 3 hours by clear water and then soaked for 3 hours by a mixed medicament A, wherein the mixed medicament A comprises 100mg/L gamma-aminobutyric acid, 10mg/L salicylic acid and 100mg/L hypersensitive protein;
(2) wrapping the seeds soaked in the step (1) with wet double-layer gauze or cotton balls, and culturing in a constant-temperature incubator at 30-35 ℃ for 18h for accelerating germination;
(3) mixing the seeds subjected to germination acceleration in the step (2) with a proper amount of insecticidal powder (the content of cypermethrin is 0.25%) and seed treatment dry powder (the content of metalaxyl is 35%), sowing on a seedling tray, and then placing in a seedling shed for seedling for about 40 days;
(4) transplanting the cherry tomato seedlings bred in the step (3) to a field for conventional production, cultivation and management;
(5) spraying the mixed agent B on the fruits in the cherry tomato small fruit period (50 d after seedling transplantation) and the color conversion period (100 d after seedling transplantation) which are transplanted to the field in the step (4); the mixed medicament B comprises 100mg/L gamma-aminobutyric acid, 10mg/L salicylic acid, 200mg/L compound sodium nitrophenolate and an emulsifier avermectin 4209-A phoxim with the percentage concentration of 0.05 percent;
(6) picking the cherry tomato fruits with the surfaces completely red after the field treatment in the step (5), putting the cherry tomato fruits into a foam box, and storing the foam box in a normal-temperature storage at the temperature of (25 +/-1) DEG C.
Embodiment 2 a pre-harvest application method for improving cherry tomato preservative and fresh-keeping effects
(1) Before seedling and sowing of cherry tomatoes, dry seeds are soaked for 4 hours by clear water and then soaked for 2 hours by a mixed medicament A, wherein the mixed medicament A comprises 200mg/L gamma-aminobutyric acid, 20mg/L salicylic acid and 200mg/L hypersensitive protein;
(2) wrapping the seeds soaked in the step (1) with wet double-layer gauze or cotton balls, and culturing in a constant-temperature incubator at 30-35 ℃ for 18h for accelerating germination;
(3) mixing the seeds subjected to germination acceleration in the step (2) with a proper amount of insecticidal powder (the content of cypermethrin is 0.25%) and seed treatment dry powder (the content of metalaxyl is 35%), sowing on a seedling tray, and then placing in a seedling shed for seedling for about 40 days;
(4) transplanting the cherry tomato seedlings bred in the step (3) to a field for conventional production, cultivation and management;
(5) spraying the mixed agent B on the fruits in the cherry tomato small fruit period (50 d after seedling transplantation) and the color conversion period (100 d after seedling transplantation) which are transplanted to the field in the step (4); the mixed medicament B comprises 200mg/L gamma-aminobutyric acid, 20mg/L salicylic acid, 500mg/L compound sodium nitrophenolate and 0.1% of emulsifier avermectin 4209-A phoxim by volume percentage;
(6) picking the cherry tomato fruits with the surfaces completely red after the field treatment in the step (5), putting the cherry tomato fruits into a foam box, and storing the foam box in a normal-temperature storage at the temperature of (25 +/-1) DEG C.
Embodiment 3 pre-harvest application method for improving preservative and fresh-keeping effects of cherry tomatoes
(1) Before seedling and sowing of cherry tomatoes, dry seeds are soaked for 4 hours by clear water and then soaked for 2 hours by a mixed medicament A, wherein the mixed medicament A comprises 1000mg/L gamma-aminobutyric acid, 40mg/L salicylic acid and 500mg/L hypersensitive protein;
(2) wrapping the seeds soaked in the step (1) with wet double-layer gauze or cotton balls, and culturing in a constant-temperature incubator at 30-35 ℃ for 18h for accelerating germination;
(3) mixing the seeds subjected to germination acceleration in the step (2) with a proper amount of insecticidal powder (the content of cypermethrin is 0.25%) and seed treatment dry powder (the content of metalaxyl is 35%), sowing on a seedling tray, and then placing in a seedling shed for seedling for about 40 days;
(4) transplanting the cherry tomato seedlings bred in the step (3) to a field for conventional production, cultivation and management;
(5) spraying the mixed agent B on the fruits in the cherry tomato small fruit period (50 d after seedling transplantation) and the color conversion period (100 d after seedling transplantation) which are transplanted to the field in the step (4); the mixed medicament B comprises 1000mg/L gamma-aminobutyric acid, 40mg/L salicylic acid, 500mg/L compound sodium nitrophenolate and 0.1% of emulsifier avermectin 4209-A phoxim by volume percentage;
(6) picking the cherry tomato fruits with the surfaces completely red after the field treatment in the step (5), putting the cherry tomato fruits into a foam box, and storing the foam box in a normal-temperature storage at the temperature of (25 +/-1) DEG C.
Embodiment 4 a pre-harvest application method for improving cherry tomato preservative and fresh-keeping effects
(1) Before seedling and sowing of cherry tomatoes, soaking dry seeds for 5 hours by using clear water, and then soaking the dry seeds for 1 hour by using a mixed medicament A; the mixed medicament A comprises 2000mg/L gamma-aminobutyric acid, 100mg/L salicylic acid and 500mg/L hypersensitivity protein;
(2) wrapping the seeds soaked in the step (1) with wet double-layer gauze or cotton balls, and culturing in a constant-temperature incubator at 30-35 ℃ for 18h for accelerating germination;
(3) mixing the seeds subjected to germination acceleration in the step (2) with a proper amount of insecticidal powder (the content of cypermethrin is 0.25%) and seed treatment dry powder (the content of metalaxyl is 35%), sowing on a seedling tray, and then placing in a seedling shed for seedling for about 40 days;
(4) transplanting the cherry tomato seedlings bred in the step (3) to a field for conventional production, cultivation and management;
(5) spraying the mixed agent B on the fruits in the cherry tomato small fruit period (50 d after seedling transplantation) and the color conversion period (100 d after seedling transplantation) which are transplanted to the field in the step (4); the mixed medicament B comprises 2000mg/L gamma-aminobutyric acid, 100mg/L salicylic acid, 1000mg/L compound sodium nitrophenolate and 0.2% of emulsifier avermectin 4209-A phoxim by volume percentage;
(6) picking the cherry tomato fruits with the surfaces completely red after the field treatment in the step (5), putting the cherry tomato fruits into a foam box, and storing the foam box in a normal-temperature storage at the temperature of (25 +/-1) DEG C.
Comparative example 1 cherry tomato preservation method
(1) Before seedling and sowing of cherry tomatoes, soaking dry seeds for 6 hours by using clear water;
(2) wrapping the seeds soaked in the step (1) with wet double-layer gauze or cotton balls, and culturing in a constant-temperature incubator at 30-35 ℃ for 18h for accelerating germination;
(3) mixing the seeds subjected to germination acceleration in the step (2) with a proper amount of insecticidal powder (the content of cypermethrin is 0.25%) and seed treatment dry powder (the content of metalaxyl is 35%), sowing on a seedling tray, and then placing in a seedling shed for seedling for about 40 days;
(4) transplanting the cherry tomato seedlings bred in the step (3) to a field for conventional production, cultivation and management;
(5) and (4) spraying an emulsifier (abamectin 4209-A phoxim) with the volume percentage concentration of 0.1% in the small fruit period (50 d after seedling transplantation) and the color conversion period (100 d after seedling transplantation) of the cherry tomatoes transplanted in the field in the step (4).
(6) Picking the cherry tomato fruits with the surfaces completely red after the field treatment in the step (5), putting the cherry tomato fruits into a foam box, and storing the foam box in a normal-temperature storage at the temperature of (25 +/-1) DEG C.
Comparative example 2 cherry tomato preservation method
(1) Before seedling and sowing of cherry tomatoes, soaking dry seeds for 6 hours by using clear water;
(2) wrapping the seeds soaked in the step (1) with wet double-layer gauze or cotton balls, and culturing in a constant-temperature incubator at 30-35 ℃ for 18h for accelerating germination;
(3) mixing the seeds subjected to germination acceleration in the step (2) with a proper amount of insecticidal powder (the content of cypermethrin is 0.25%) and seed treatment dry powder (the content of metalaxyl is 35%), sowing on a seedling tray, and then placing in a seedling shed for seedling for about 40 days;
(4) transplanting the cherry tomato seedlings bred in the step (3) to a field for conventional production, cultivation and management;
(5) spraying the mixed agent B on the fruits in the cherry tomato small fruit period (50 d after seedling transplantation) and the color conversion period (100 d after seedling transplantation) which are transplanted to the field in the step (4); the mixed medicament B comprises 1000mg/L gamma-aminobutyric acid, 40mg/L salicylic acid, 500mg/L compound sodium nitrophenolate and 0.1% of emulsifier avermectin 4209-A phoxim by volume percentage;
(6) picking the cherry tomato fruits with the surfaces completely red after the field treatment in the step (5), putting the cherry tomato fruits into a foam box, and storing the foam box in a normal-temperature storage at the temperature of (25 +/-1) DEG C.
TABLE 3 Effect of the treatments before harvesting on the preservative and freshness-keeping effects of cherry tomatoes in the examples and comparative examples (storage 15d)
Figure BDA0003239721910000081
Figure BDA0003239721910000091
The results show that: by adopting the embodiment method, after being stored for 15 days, the rotting rate of the cherry tomato fruits is 1.73-5.21%, which is 15.89-19.37% lower than that of the control fruits (21.10%), and the control effect reaches 75.31-91.80%, thus the processing method of the embodiments 1-4 can improve the preservative and fresh-keeping effects of the cherry tomato fruits.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.

Claims (9)

1. The pre-harvest application method for improving the preservative and fresh-keeping effects of cherry tomatoes is characterized by comprising the following steps of:
(1) before seedling and sowing of cherry tomatoes, soaking dry seeds in water, and then soaking the seeds in a mixed medicament A;
the mixed medicament A comprises gamma-aminobutyric acid, salicylic acid and hypersensitive protein;
(2) spraying the mixed medicament B to the fruits in the small fruit period and the color transition period of the cherry tomatoes;
the mixed medicament B comprises gamma-aminobutyric acid, salicylic acid, compound sodium nitrophenolate and an emulsifier.
2. The method for improving the preservation and fresh-keeping effects of cherry tomatoes as claimed in claim 1, wherein the mixed agent A comprises 100-2000 mg/L gamma-aminobutyric acid, 10-100 mg/L salicylic acid and 100-500 mg/L hypersensitive protein.
3. The method for improving the preservative and fresh-keeping effects of cherry tomatoes as claimed in claim 1, wherein the mixed medicament B comprises 100-2000 mg/L of gamma-aminobutyric acid, 10-100 mg/L of salicylic acid, 200-1000 mg/L of compound sodium nitrophenolate and 0.05-0.2% of emulsifier by volume percentage.
4. The method for improving the preservation and fresh-keeping effects of cherry tomatoes as claimed in claim 1, wherein the mixed agent A comprises 1000mg/L gamma-aminobutyric acid, 40mg/L salicylic acid and 500mg/L hypersensitive protein.
5. The method for improving the preservation and fresh-keeping effects of cherry tomatoes as claimed in claim 1, wherein the mixed medicament B comprises 1000mg/L gamma-aminobutyric acid, 40mg/L salicylic acid, 500mg/L compound sodium nitrophenolate and 0.1% by volume of emulsifier.
6. The pre-harvest application method for improving the preservative and fresh-keeping effects of cherry tomatoes according to claim 1, characterized in that the emulsifier is abamectin 4209-A phoxim.
7. The pre-harvest application method for improving the preservative and fresh-keeping effects of cherry tomatoes as claimed in claim 1, wherein the seed soaking time with water is 3-5 h.
8. The pre-harvest application method for improving the preservative and fresh-keeping effects of cherry tomatoes as claimed in claim 1, wherein the seed soaking time of the mixed agent A is 1-3 h.
9. The pre-harvest application method for improving the cherry tomato preservative and fresh-keeping effects is characterized in that the small fruit period is 50-60 days after seedling transplantation, and the color conversion period is 100-110 days after seedling transplantation.
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