CN113834798A - Multifunctional optical detection and analysis system - Google Patents
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
The invention provides a multifunctional optical detection and analysis system, which comprises: the device comprises an optical path detection unit, a sample processing unit, an optical path sorting unit, a signal conversion unit and a data processing unit. The multifunctional optical detection and analysis system provided by the invention can realize the detection of various substances when a liquid phase sample is only injected once, wherein the detection of different optical analysis methods is involved. Meanwhile, the method can automatically control the moving and the processing of the liquid phase sample, distribute the original sample, the preprocessed sample and the mixed sample into a detection light path according to a set program, automatically select a corresponding light path configuration and an optical analysis method, realize the quantitative detection of various different substances in the same liquid phase sample, and ensure that the multinomial combined analysis is simpler, more convenient, more reliable, more efficient and safer.
Description
Technical Field
The invention relates to the technical field of detection and analysis, in particular to a multifunctional optical detection and analysis system.
Background
Optical analysis refers to a method for chemical analysis by using the optical properties of substances, and commonly used optical analysis includes fluorescence photometric analysis, transmission light analysis, scattered light analysis and chemiluminescence analysis, and the analysis methods have mature technologies, more types of analyzable substances and higher automation level, and are widely applied to the fields of health inspection, environmental and food analysis, drug analysis, biochemistry, clinical detection and the like.
However, most of the analysis apparatuses currently mainly use an analysis apparatus equipped with an analysis method, such as: a full-automatic biochemical analyzer based on transmitted light analysis and a full-automatic luminescence immunoassay analyzer based on chemiluminescence analysis. In actual scientific research and production work, detection and analysis of multiple different substances are often required to be performed on the same sample. Depending on the limitations of the detection method of the analyzer, even the detection and analysis of the substance of interest in the same sample often requires the use of a plurality of different analyzers.
In addition, in many cases, a solution sample subjected to pretreatment such as centrifugation or dissolution is used for the detection analysis. The process of sample pretreatment requires extensive manual intervention by laboratory personnel and is a major limiting factor in laboratory automation, while sample exposure during pretreatment is one of the major bio-safety risks in the laboratory.
Disclosure of Invention
In view of the above, the present invention provides a multifunctional optical detection and analysis system to solve the problem of single function of the detection and analysis device.
In view of the above object, the present invention provides a multifunctional optical detection and analysis system, comprising:
the light path detection unit comprises at least two groups of detection light paths, and each group of detection light paths comprises a weak light detection light path and a transmission light detection light path;
the sample processing unit is communicated with the light path detection unit through a pipeline and is used for moving, taking and processing a sample;
the optical path sorting unit is used for providing detection light beams for the optical path detection unit, comprises at least two groups of sorting optical paths, and is connected with the optical path detection unit through an optical fiber;
the signal conversion unit is electrically connected with the optical path detection unit and is used for performing signal conversion on the optical signal output by the optical path detection unit and outputting a digital signal;
and the data processing unit is electrically connected with the signal conversion unit and used for analyzing and processing the digital signals output by the signal conversion unit and outputting analysis data.
Further, the weak light detection optical path is used for detecting scattered light or fluorescence of the sample, and the transmitted light detection optical path is used for detecting transmitted light of the sample.
Further, the detection pond in the detection light path includes detection zone and capillary, the capillary sets up in the detection zone, the capillary is the wave form capillary, the one end of capillary is the inflow end, and the other end is the outflow end.
Further, the output optical signal of the weak light detection optical path is filtered by a first optical filter and then sent to the photoelectric conversion unit, the first optical filter is assembled in a first circular filter wheel, and the first circular filter wheel is driven by a first stepping motor.
Further, the sample processing unit includes an original sample sampling path, a preprocessed sample sampling path, and at least one mixed sample sampling path, and samples respectively obtained through the original sample sampling path, the preprocessed sample sampling path, and the mixed sample sampling path are detected by the optical path detecting unit.
Further, the sample processing unit further includes: the cleaning device comprises a cleaning passage and a compressed air passage, wherein the pretreatment sample sampling passage and the mixed sample sampling passage are respectively communicated with the cleaning passage, and the pretreatment sample sampling passage and the mixed sample sampling passage are respectively communicated with the compressed air passage.
Further, the sample processing unit includes an original sample sampling path and a multiple sample sampling path, and samples obtained through the original sample sampling path and the multiple sample sampling path are detected by the optical path detecting unit.
Furthermore, the multi-sample sampling path comprises a reaction disc provided with at least two reaction cups, and the samples in the reaction cups are detected through the light path detection unit.
Furthermore, the detection light path for detecting the sample in the reaction cup comprises a detection module, and a detection groove matched with the reaction disc is formed in the detection module.
Further, the signal conversion unit includes a photoelectric conversion element and an analog-to-digital conversion element, the photoelectric conversion element converts the optical signal output by the optical path detection unit into an electrical signal, and the analog-to-digital conversion element converts the electrical signal into a digital signal.
From the above, the multifunctional optical detection and analysis system provided by the invention can realize the detection of various substances when a liquid phase sample only needs to be fed once, wherein the detection of different optical analysis methods is involved. The system provided by the invention can automatically control the moving and processing of the liquid phase sample, distribute the original sample, the preprocessed sample and the mixed sample into the detection light path according to a set program, automatically select the corresponding light path configuration and the optical analysis method, realize the quantitative detection of various different substances in the same liquid phase sample, and ensure that the multinomial combined analysis is simpler, more convenient, more reliable, more efficient and safer.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a multifunctional optical detection and analysis system according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of an optical path detecting unit according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a sample processing unit according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a detection cell according to an embodiment of the present invention;
FIG. 5 is a schematic structural diagram of a sample processing unit according to another embodiment of the present invention;
FIG. 6 is a schematic structural view of a reaction disk according to an embodiment of the present invention;
FIG. 7 is a schematic structural diagram of an optical path detecting unit according to another embodiment of the present invention;
fig. 8 is a schematic structural diagram of an optical path sorting unit according to an embodiment of the present invention.
Reference numerals:
1. an optical path detection unit; 11. a first collimator; 12. a first detection cell; 121. an inflow end; 122. an outflow end; 123. a capillary tube; 124. a detection zone; 13. a first lens; 141. a first optical filter; 142. a first circular filter wheel; 15. a first stepper motor; 16. a first fiber optic interface; 17. a first optical fiber; 18. a detection module; 181. a detection tank; 182. a second collimator; 183. a second lens; 184. a second optical fiber interface; 185. a third optical fiber interface; 186. a fourth optical fiber interface; 187. a third lens; 188. a third optical filter; 189. a third circular filter wheel; 1891. a third step motor; 2. a sample processing unit; 211. a first original sample tube; 212. a first sampling needle; 213. a first rotary sampling valve; 214. a first selector valve; 215. a first plunger pump; 216. a first quantity of sample loops; 221. a first reagent bottle; 222. a first reagent pump; 223. a first mixing tank; 224. a second rotary sampling valve; 2241. a second quantitative sample loop; 225. a third rotary sampling valve; 2251. a third quantitative sample loop; 226. a second selector valve; 227. a second detection cell; 228. a second plunger pump; 231. a second reagent bottle; 232. a second reagent pump; 233. a second mixing tank; 241. a third reagent bottle; 242. a third reagent pump; 243. a third mixing tank; 251. an air compression pump; 252. a first air solenoid valve; 253. a second air solenoid valve; 254. a third air solenoid valve; 261. a cleaning solution reagent bottle; 262. a first cleaning solenoid valve; 263. cleaning the pump; 264. a second cleaning solenoid valve; 265. a third cleaning solenoid valve; 266. a fourth cleaning solenoid valve; 267. a waste discharge pump; 268. a fifth cleaning electromagnetic valve; 271. a reaction disc; 2711. a reaction cup; 2712. a sample application system; 2713. a reagent dispensing system; 272. a second sampling needle; 273. a fourth rotary sampling valve; 2731. a fourth quantitative sample loop; 274. a third selector valve; 275. a third detection cell; 276. a third plunger pump; 277. a fourth reagent bottle; 278. a fourth reagent pump; 281. a second original sample tube; 282. a third sampling needle; 3. an optical path sorting unit; 31. a light source; 32. a fifth optical fiber interface; 33. a third collimator; 34. a second optical filter; 341. a second circular filter wheel; 35. a sixth optical fiber interface; 36. a second optical fiber; 37. a second stepping motor; 4. a signal conversion unit; 5. a data processing unit.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to specific embodiments and the accompanying drawings.
It is to be noted that technical terms or scientific terms used in the embodiments of the present invention should have the ordinary meanings as understood by those having ordinary skill in the art to which the present disclosure belongs, unless otherwise defined. The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
The multifunctional optical detection and analysis system provided by the invention is described in detail below with reference to the accompanying drawings.
Referring to fig. 1, the present invention provides a multifunctional optical detection and analysis system, including:
the light path detection unit 1 comprises at least two groups of detection light paths, each group of detection light paths comprises a weak light detection light path and a transmission light detection light path, multiple kinds of light analysis can be carried out on a sample, and the two groups of detection light paths are mutually independent. The light path structures of the two groups of detection light paths can be set to be the same or different and are adjusted according to specific detection conditions. The number of the detection light paths is not limited to two, and a plurality of detection light paths can be arranged, so that the optical detection analysis can be carried out on the sample at the same time or in a time-sharing manner.
The sample processing unit 2 is communicated with the light path detection unit 1 through a pipeline and used for moving, taking and processing samples, automatically sampling and preprocessing original samples, mixing the samples with other different reagents, automatically preparing a plurality of mixed samples, reducing manual intervention, reducing manual workload and simultaneously reducing sample exposure time.
The light path sorting unit 3 is used for providing detection light beams for the light path detection unit 1, and comprises at least two groups of sorting light paths, the light path sorting unit 3 is connected with the light path detection unit 1 through an optical fiber, a light source of the light path sorting unit 3 is a wide-spectrum or multi-spectrum light source, and a spectrum light source or a monochromatic light source meeting detection requirements can be output through conversion of an optical element.
And the signal conversion unit 4 is electrically connected with the optical path detection unit 1 and is used for performing signal conversion on the optical signal output by the optical path detection unit 1 to output a digital signal, converting the optical signal through a signal conversion element in the signal conversion unit 4, and converting the optical signal into the digital signal so as to be analyzed and processed by the data processing unit 5.
And the data processing unit 5 is electrically connected with the signal conversion unit 4 and used for analyzing and processing the digital signals output by the signal conversion unit 4, obtaining the concentration of the detected object in the detected sample according to a light intensity data-concentration algorithm of the corresponding detected object by a computer and special software, completing optical analysis, outputting analysis data and completing detection of the specified substance in the original sample put into the detection and analysis system.
In some embodiments, the weak light detection optical path is used for detecting scattered light or fluorescence of the sample, and the transmitted light detection optical path is used for detecting transmitted light of the sample. Referring to fig. 1, monochromatic light output from the light path sorting unit 3 is collimated and then irradiated onto the surface of a sample to be detected, and the monochromatic light is used as excitation light for performing fluorescence photometric analysis on the sample to be detected or as an incident light source for transmission light and scattered light analysis. Fluorescence emitted by a sample to be detected after excitation and scattered light generated by reflecting incident light are detected through a weak light detection optical path, and transmitted light formed by the sample to be detected after irradiation of the incident light is detected through a transmission light detection optical path.
In some embodiments, the detection cell in the detection optical path includes a detection region 124 and a capillary tube 123, the capillary tube 123 is disposed in the detection region 124, the capillary tube 123 is a corrugated capillary tube, one end of the capillary tube 123 is an inflow end 121, and the other end is an outflow end 122.
The first detection cell 12 and the second detection cell 227 in this embodiment are flow detection cells, as shown in fig. 4. The flow detection cell is a flat rectangular quartz glass or transparent polymer material product, and comprises a detection area 124 and a capillary 123 therein, the capillary 123 is a corrugated capillary, two ends of the capillary 123 are respectively an inflow end 121 of liquid and an outflow end 122 of liquid, and the inflow end 121 and the outflow end 122 are connected in series in a fluid processing pipeline and are used for inflow and outflow of fluids such as samples. The detection zone 124 refers to the smallest enveloping circular area of the capillary 123. The capillary 123 has a uniform and consistent inner diameter, with an applicable size of 0.1mm to 1.6 mm. The area ratio of the total length of the capillary 123 to the minimum enveloping circle, i.e., the detection area 124, is greater than or equal to 0.3mm/mm2. Compared with a linear capillary tube, the waveform capillary tube has larger liquid volume, can store more detection samples, increases the absorption or reflection area of the samples to light in the detection process, and can effectively improve the optical detection precision.
The flow cell in the present embodiment is suitable for detecting various low-viscosity liquid samples, and is not limited to the application form in the present embodiment, and may be used in other devices such as fluorescence analysis, luminescence analysis, scattered light analysis, transmission light detection, and image analysis.
In some embodiments, referring to fig. 2, the output light signal of the weak light detection optical path is filtered by a first optical filter 141 and then sent to the signal conversion unit 4, the first optical filter 141 is assembled in a first circular filter wheel 142, and the first circular filter wheel 142 is driven by a first stepper motor 15. Specifically, the light path detecting unit 1 includes two sets of the same detection light paths, the weak light detection light path of each set of detection light path includes a first collimator 11, a first detection cell 12, a first lens 13 and a first optical filter 141 in order, the incident light emitted by the light path sorting unit 3 irradiates the surface of the sample to be detected of the first detection cell 12 after being collimated by the first collimator 11, the light emitted from the first detection cell 12 converges and collimates through the first lens 13, and irradiates the signal conversion element of the signal conversion unit 4 after being filtered by the first optical filter 141. The first filter 141 is mounted on the first circular filter wheel 142, and a plurality of sets of filters with different wavelengths can be mounted in the first circular filter wheel 142, so that the first filters 141 with different wavelengths can be selected by rotating the first circular filter wheel 142. The first circular filter wheel 142 is driven to rotate by the first stepping motor 15, the first stepping motor 15 is controlled by the system to operate, the first circular filter wheel 142 is driven to rotate according to the light path parameter setting corresponding to the sample to be detected in the detection cell, and the first optical filter 141 installed in the first circular filter wheel 142 is selected to be in the detection light path, so that the optical detection of the weak light detection light path is completed. The transmitted light detection optical path includes, in order, a first collimator 11, a first detection cell 12, and a first fiber interface 16. Incident light shines first detection cell 12 after first collimator 11, forms the transmitted light at the opposite side of first detection cell 12, and this transmitted light passes through first optical fiber interface 16, transmits to signal conversion unit 4 through first optic fibre 17 again, and first optical fiber interface 16 in this embodiment is microscope optical fiber interface.
The two groups of detection light paths in the light path detection unit 1 can realize the detection of the characteristic optical data of two different samples. In this embodiment, the characteristic optical data detection includes fluorescence detection, transmitted light detection, and scattered light detection, and the detection light path may also be adjusted according to actual detection requirements to detect other characteristic optical data, and is not limited to the three characteristic optical data provided in this embodiment.
In some embodiments, the sample processing unit 2 includes an original sample sampling path, a preprocessed sample sampling path, and at least one mixed sample sampling path, and samples respectively obtained through the original sample sampling path, the preprocessed sample sampling path, and the mixed sample sampling path are all detected by the optical path detecting unit 1. The sample processing unit 2 can detect an original sample, and can also detect a pre-processed sample and a mixed sample, so that detection of various substances can be realized. One mixed sample sampling passage corresponds to one mixed sample, and the mixed sample sampling passage may be added according to actual conditions, and is not limited to one mixed sample sampling passage in the present embodiment.
In some embodiments, the sample processing unit 2 further comprises: the cleaning device comprises a cleaning passage and a compressed air passage, wherein the pretreatment sample sampling passage and the mixed sample sampling passage are respectively communicated with the cleaning passage, and the pretreatment sample sampling passage and the mixed sample sampling passage are respectively communicated with the compressed air passage. The cleaning passage can clean the mixing tank of the preprocessed sample and the mixed sample, and the compressed air passage can fully mix the sample in the mixing tank by conveying compressed air, so that the sample detection precision is improved.
Referring to fig. 3, the sample processing unit 2 includes an original sample sampling path, a preprocessed sample sampling path, two mixed sample sampling paths, a purge path, and a compressed air path. The original sample sampling passage sequentially comprises a first original sample tube 211, a first sampling needle 212, a first rotary sampling valve 213, a first selection valve 214, a first detection cell 12 and a first plunger pump 215, the first rotary sampling valve 213 rotates to a sampling position, the sampling needle 212 is driven by a motor to enter the original sample tube 211, the original sample is sucked from the original sample tube 211 through the original sample sampling passage, one part of the sucked original sample enters the first detection cell 12 for detection, the other part of the original sample is filled into a first quantitative sample ring 216 in the first rotary sampling valve 213, and at this time, the sampling is completed, and the sampling needle 212 is driven by the motor to move out of the sample tube. After the original sample in the first detection cell 12 is detected, the first plunger pump 215 is used to drive the first detection cell 12 to fill pure water, the first detection cell 12 is cleaned, and the cleaned waste water is driven by the first plunger pump 215 to be discharged from the waste discharge port of the first selection valve 214 for the next sample suction detection.
The pre-processed sample sampling path sequentially includes a first reagent bottle 221, a first reagent pump 222, a first rotary sampling valve 213, a first mixing tank 223, a second rotary sampling valve 224, a third rotary sampling valve 225, a second selector valve 226, a second detection tank 227 and a second plunger pump 228, specifically, the first rotary sampling valve 213 rotates to a dispensing position, the first reagent pump 222 discharges pre-processed liquid drawn from the first reagent bottle 221, pushes the raw samples filled in the first quantitative sample ring 216 to be sequentially injected into the first mixing tank 223, the pre-processed liquid is mixed with the raw samples to be pre-processed samples, the second rotary sampling valve 224 and the third rotary sampling valve 225 rotate to a sampling position, the pre-processed sample sampling path draws the pre-processed samples from the first mixing tank 223, a part of the drawn pre-processed samples enters the second detection tank 227 for detection, another part of the second quantitative sample ring 2241 and the third quantitative sample ring 2251 in the second rotary sampling valve 224 and the third rotary sampling valve 225, sampling of the pre-treated sample is completed. When the detection of the pretreated sample in the second detection cell 227 is completed, pure water is injected into the second detection cell 227 by the pushing of the second plunger pump 228 for cleaning, and the cleaned wastewater is discharged from the second selection valve 226 by the pushing of the second plunger pump 228, so that the second detection cell 227 is flushed for the next suction detection of the sample.
The sample sampling path of the first mixed sample includes a second reagent bottle 231, a second reagent pump 232, a second rotary sampling valve 224, a second mixing well 233, a second selection valve 226, a second detection well 227, and a second plunger pump 228 in order, and the sample sampling path of the second mixed sample includes a third reagent bottle 241, a third reagent pump 242, a third rotary sampling valve 225, a third mixing well 243, a second selection valve 226, a second detection well 227, and a second plunger pump 228 in order. The second rotary sampling valve 224 and the third rotary sampling valve 225 are rotated to the dispensing position, the second reagent pump 232 discharges the reagent sucked from the second reagent bottle 231, the pre-processed samples filled in the second quantitative sample ring 2241 are pushed to be sequentially injected into the second mixing pool 233, the third reagent pump 242 discharges the reagent sucked from the third reagent bottle 241, the pre-processed samples filled in the third quantitative sample ring 2251 are pushed to be sequentially injected into the third mixing pool 243, and the reagent and the pre-processed samples are uniformly mixed to form a mixed sample. Through the pipeline connected to the second selector valve 226, the mixed sample in the second mixing pool 233 and the third mixing pool 243 is sequentially sucked by the second plunger pump 228 through the second selector valve 226 and then filled into the second detection pool 227 for detection.
In this embodiment, the pre-processed sample in the first mixing cell 223, the mixed sample in the second mixing cell 233, and the mixed sample in the third mixing cell 243 may also be respectively communicated with the first selector valve 214 through a pipeline, and sucked and filled into the first detection cell 12 through the first plunger pump 215 for detection.
The air compression passage includes an air compression pump 251 and first 252, second 253, and third 254 air solenoid valves in the passage. The first air solenoid valve 252 is pulsed on a plurality of times, and a small amount of compressed air is pumped into the first mixing tank 223 by the air compressor 251 to mix the pre-processed sample thoroughly. After the second air solenoid valve 253 and the third air solenoid valve 254 are pulsed for a plurality of times, a small amount of compressed air is pulsed into the second mixing tank 233 and the third mixing tank 243 by the air compressor pump 251, so that the mixed samples are fully and uniformly mixed.
The cleaning path includes a cleaning solution reagent bottle 261, a first cleaning solenoid valve 262, a cleaning pump 263, a fifth cleaning solenoid valve 268, a second cleaning solenoid valve 264, a third cleaning solenoid valve 265, a fourth cleaning solenoid valve 266 and a waste discharge pump 267, the second cleaning solenoid valve 264, the third cleaning solenoid valve 265 and the fourth cleaning solenoid valve 266 which are connected with the waste discharge pump 267 and correspond to each other are respectively connected, the waste discharge pump 267 is started to empty the residual liquid in the relevant first mixing tank 223, the second mixing tank 233 and the third mixing tank 243, then the fifth solenoid valve 268 and the cleaning solenoid valves connected with the mixing tanks are connected, the cleaning solution in the cleaning solution reagent bottle 261 is injected into the mixing tanks through the cleaning pump 263, and then the mixing tanks are emptied to complete one cleaning. The first solenoid valve 262 is connected, pure water is injected into each mixing pool through the cleaning pump 263, and then each mixing pool is emptied one after another, thereby completing one rinsing. And the cleaned and rinsed mixed tanks can be used for mixing treatment of the next sample.
The mixing pool inner cavity in this embodiment is cylindrical or elliptic cylindrical, and each mixing pool at least includes 4 pipe connection ports, which are a sample moving-in port, a sample taking-out port, an air inlet port, and an evacuation/purge port, respectively. Except for the connecting port, the mixing tank in the embodiment is of a fully-closed structure.
In some embodiments, the sample processing unit 2 includes an original sample sampling path and a multiple sample sampling path, and samples obtained through the original sample sampling path and the multiple sample sampling path respectively are detected by the optical path detecting unit 1. Specifically, referring to fig. 5, the original sample sampling path is not changed, and the pre-treatment sample sampling path and the mixed sample sampling path are replaced with a multi-sample sampling path, and the multi-sample sampling path sequentially includes a fourth reagent bottle 277, a fourth reagent pump 278, a fourth rotary sampling valve 273, a second sampling needle 272, and a reaction disk 271. The raw sample sampling path in this embodiment includes, in order, a second raw sample tube 281, a third sampling needle 282, a fourth rotary sampling valve 273, a third selector valve 274, a third detection cell 275, and a third plunger pump 276. The fourth rotary sampling valve 273 is rotated to the sampling position, the motor drives the third sampling needle 282 to enter the second original sample tube 281, the original sample is sucked from the second original sample tube 281, a part of the sucked original sample enters the third detection cell 275 for detection, and the other part of the sucked original sample is filled in the fourth quantitative sample ring 2731 in the fourth rotary sampling valve 273, so that the sampling is completed, and the motor drives the third sampling needle 282 to move out of the second original sample tube 281. After the original sample in the third detection cell 275 is detected, pure water is filled in the third plunger pump 276 for cleaning, and the cleaned waste water is pushed by the third plunger pump 276 to be discharged from the waste discharge port of the third selection valve 274, so that the third detection cell 275 is cleaned for the next sample to be detected to be sucked and detected.
The fourth rotary sampling valve 273 is rotated to the dispensing position, the fourth reagent pump 278 discharges the pretreatment liquid sucked from the fourth reagent bottle 277, pushes the original sample filled in the fourth quantitative sample ring 2731 to be filled into the reaction disk 271 together to be mixed into a pretreatment sample, and then the pretreatment sample and the reagent are mixed uniformly to prepare a mixed sample. The reaction disk 271 is driven by an external motor to rotate, so as to rotate the pre-processed sample and the mixed sample which are subjected to reaction to the optical path detection module 18 composed of optical elements to complete characteristic light detection, and the optical path detection module 18 is contained in the optical path detection unit 1.
In some embodiments, referring to fig. 6, the multi-sample sampling path includes a reaction disk 271 having at least two reaction cups 2711, and the sample in the reaction cups 2711 is detected by the optical path detecting unit 1. Specifically, the reaction disk 271 includes a plurality of reaction cups 2711 circumferentially disposed at equal intervals on the edge of the reaction disk 271, and each reaction cup 2711 also serves as a detection cup. The reaction disk 271 is matched with the sample adding system 2712 and the reagent distributing system 2713 to sample and distribute the pretreated sample for multiple times, and different reagents are sequentially added into the reaction cup 2711 to be mixed and reacted to form a plurality of mixed samples. The sample and the pretreatment liquid injected from the fourth rotary sampling valve 273 are stored in one of the cuvettes 2711 to be mixed into a pretreated sample, the sample injection system 2712 samples the sample from the cuvette 2711 a plurality of times and redistributes the sample to the other cuvettes 2711, and different reaction reagents are injected into the sample injection system 2713, respectively, thereby forming a plurality of mixed samples.
In some embodiments, the detection light path for detecting the sample in the reaction cup 2711 includes a detection module 18, and a detection groove 181 matched with the reaction disk 271 is formed in the detection module 18. Referring to fig. 7, the optical path detecting unit 1 includes two sets of detecting optical paths, where one set of detecting optical path is used for detecting the third detecting cell 275, and the detecting manner is the same as that described above, and is not described herein again. And the other set of detection light paths is used for detecting the sample in the reaction cup 2711 of the reaction disc 271. The detecting module 18 is placed under the reaction disk 271 so that the detecting cups 2711 are positioned in the detecting grooves 181 of the detecting module 18, and the width of the detecting grooves 181 is larger than the thickness of the detecting cups 2711 in the reaction disk 271, thereby ensuring that each detecting cup 2711 in the reaction disk 271 can move and detect in the detecting groove 181 smoothly. The detection module 18 includes a second collimator 182, a second lens 183, a second optical fiber interface 184, and a third optical fiber interface 185, wherein the second collimator 182, the second lens 183, and the second optical fiber interface 184 are included in the weak light detection optical path, and the third optical fiber interface 185 is included in the transmission light detection optical path. Incident light from the light path sorting unit 3 is collimated by the second collimator 182 and then irradiates to a sample surface in one detection cup 2711 of the reaction disk 271, scattered light generated by reflection of the sample or fluorescence generated by excitation of the sample is converged and collimated by the second lens 183 and then enters the second optical fiber interface 184, and then enters the third optical filter 188 through convergence and collimation of the fourth optical fiber interface 186 and the third lens 187, and light emitted by the third optical filter 188 irradiates to the signal conversion unit 4 for signal conversion. The third filter 188 is fitted in a third circular filter wheel 189, and the third circular filter wheel 189 is driven by a third stepper motor 1891. The combined use of the detection cell and the detection cup in the embodiment is suitable for the detection requirements of combining multi-stage processing and different optical analysis methods, and compared with the use of a single detection cell, more reagent mixed samples can be conveniently prepared, and more substances can be quantitatively detected more quickly.
In some embodiments, the sorting light path includes a second filter 34 for outputting a monochromatic light source, the second filter 34 being mounted in a second circular filter wheel 341, the second circular filter wheel 341 being driven by a second stepper motor 37. Referring to fig. 8, the optical path sorting unit 3 includes two sets of sorting optical paths, which are identical in configuration in this embodiment. Each group of sorting optical paths includes a light source 31, a fifth optical fiber interface 32, a third collimator 33, a second optical filter 34, and a sixth optical fiber interface 35. The light source 31 is a wide-spectrum or multi-spectrum light source, and the wide-spectrum light emitted by the light source 31 is transmitted to the third collimator 33 through the fifth optical fiber interface 32 and the second optical fiber 36, is divided into two paths of wide-spectrum light to be output, and is collimated into two parallel light beams by the third collimator 33. And then filtered by the second optical filter 34 to form two monochromatic lights, where the two monochromatic lights respectively irradiate onto the light receiving surfaces of the two sixth optical fiber interfaces 35 symmetrically arranged, and the monochromatic lights capable of being transmitted by the optical fibers are converged and output in the sixth optical fiber interfaces 35. The wavelength of the monochromatic light is determined by the second filter 34, the second filter 34 is assembled in the second circular filter wheel 341, a plurality of sets of narrow-band filters can be assembled in the second circular filter wheel 341, and the second circular filter wheel 341 is driven by the second stepper motor 37 to rotate. The light path sorting unit 3 can simultaneously output two paths of monochromatic light with different wavelengths, and the two paths of monochromatic light form a group. According to the system instruction, the optical path sorting unit 3 can output a plurality of groups of specified monochromatic light groups with different wavelengths in a time-sharing manner.
In some embodiments, the signal conversion unit 4 includes a photoelectric conversion element that converts an optical signal output from the optical path detection unit 1 into an electrical signal, and an analog-to-digital conversion element that converts the electrical signal into a digital signal. In this embodiment, the photoelectric conversion element includes a photomultiplier tube and a photodiode, the photomultiplier tube is a photodetector for weak light such as fluorescent light and scattered light, and the photodiode is a photodetector for transmitted light. The optical signal is converted into current through the photoelectric detector, then is amplified and processed, and is converted into light intensity data through the analog-to-digital conversion element A/D, and further detection of the optical data of the sample characteristics to be detected is achieved. The photomultiplier and the photodiode can be replaced by image detection devices such as CCD, CMOS and the like, so that the image analysis and the spectrum analysis of a sample to be detected can be realized.
The following describes the working procedure of the multifunctional optical detection and analysis system provided by the present invention:
the working process is as follows:
an original sample is placed in a multifunctional optical detection analysis system, and is distributed and processed into 4 samples by a sample processing unit 2, wherein the samples are respectively an original sample, a preprocessed sample and two mixed samples, the structure of the sample processing unit 2 is shown in fig. 3, and the structure of an optical path detection unit 1 is shown in fig. 2. As shown in fig. 3, the original sample and the pre-processed sample are respectively filled into the first detection cell 12 and the second detection cell 227 for detection, after the detection is completed, the first detection cell 12 and the second detection cell 227 are respectively emptied and cleaned, and then the two mixed samples are detected, and the detection method is the same as above.
The characteristic light detection of the 4 samples is performed through the light path detection unit 1, according to the characteristic wavelength of the exciting light of the sample to be detected, the light path sorting unit 3 outputs a corresponding characteristic monochromatic light group according to a system instruction, the corresponding characteristic monochromatic light group is transmitted to the light path detection unit 1, the sample to be detected is excited by the characteristic monochromatic light to generate characteristic emitting light, stray light is filtered by a corresponding optical filter selected according to the system instruction in the light path detection unit 1, the stray light is detected through a weak light detection light path, and the process is a fluorescence detection process.
According to the characteristic wavelength of the absorbed light of the sample to be detected, the light path sorting unit 3 outputs a corresponding characteristic monochromatic light group according to a system instruction, the corresponding characteristic monochromatic light group is transmitted to the light path detection unit 1, and the characteristic monochromatic light is partially absorbed by the sample to be detected. The unabsorbed transmitted light is transmitted to the signal conversion unit 4 through the first optical fiber 17 by the first optical fiber interface 16 in the optical path detection unit 1, and the intensity of the transmitted light is in direct proportion or inverse proportion to the concentration of the sample to be detected, and the transmitted light is detected through the transmitted light detection optical path.
According to the characteristic wavelength of the absorbed light of the sample to be detected, the light path sorting unit 3 outputs a corresponding characteristic monochromatic light group according to a system instruction, the corresponding characteristic monochromatic light group is transmitted to the light path detection unit 1, and the characteristic monochromatic light is scattered by the sample part to be detected. According to the system instruction, a corresponding scattered light filter set is selected in the optical path detection unit 1, and the scattered light enters the signal conversion unit 4 to be detected, wherein the process is a scattered light detection process and is detected through a weak light detection optical path.
The three optical signals are converted into digital light intensity data which can be identified, stored and called by computer software through the signal conversion unit 4. The light intensity data is further analyzed and calculated through the data processing unit 5, the concentration of the detected object in the sample to be detected is obtained, optical analysis is completed, analysis data is output, and detection of the specified substance in the original sample is completed.
In summary, in the present flow, the original samples are allocated and processed into 4 samples after being put in. The light source is sequentially filled into 2 independent detection cells for detection, at least 4 characteristic light data are detected through 2 groups of independent light paths, and quantitative detection of at least 4 different substances in the original sample is realized according to a corresponding optical analysis method.
And 2, a work flow:
the difference between the process and the work process 1 is that: an optical path detection unit 1 and a sample processing unit 2. The optical path detection unit 1 refers to fig. 7, and the sample processing unit refers to fig. 5 and 6. The original sample is placed in the sample processing unit 2, the original sample and the pretreatment solution are sampled and distributed, the difference between the workflow 1 and the original sample and the pretreatment solution is avoided, and the sample is treated by using the reaction disk 271 when the pretreatment sample is formed and the mixed sample is prepared. By the cooperation of the sample addition system 2712 and the reagent distribution system 2713, a plurality of mixed samples are formed in the cuvette 2711. After the preparation of the mixed sample is completed, the reaction disk 271 is rotated into the detection module 18 for optical detection. The reaction disk 271 is placed in the detection groove 181, and the detection of different mixed samples is completed by aligning different cuvettes 2711 into the detection groove 181 through rotation, and the detection of the pretreated sample is also completed in the cuvettes 2711. The original sample is detected in the third detection cell 275 according to the same detection principle as the working process 1.
Aiming at different samples to be detected, three different characteristic lights, namely fluorescence, transmission light and scattered light, can be respectively detected through the light path detection unit 1, corresponding characteristic optical signals are obtained through the signal conversion unit 4, and finally optical analysis is completed through the data processing unit 5, and optical data are output.
The flow adopts an analysis device combining a detection pool and a detection cup, can carry out multi-stage distribution and treatment on samples, is suitable for combining the multi-stage treatment with different optical analysis methods, and can be used for detecting specific samples of multiple substances in a combined manner.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the idea of the invention, also features in the above embodiments or in different embodiments may be combined, steps may be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.
The embodiments of the invention are intended to embrace all such alternatives, modifications and variances that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, substitutions, improvements and the like that may be made without departing from the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (10)
1. A multi-functional optical inspection analysis system, comprising:
the light path detection unit comprises at least two groups of detection light paths, and each group of detection light paths comprises a weak light detection light path and a transmission light detection light path;
the sample processing unit is communicated with the light path detection unit through a pipeline and is used for moving, taking and processing a sample;
the optical path sorting unit is used for providing detection light beams for the optical path detection unit, comprises at least two groups of sorting optical paths, and is connected with the optical path detection unit through an optical fiber;
the signal conversion unit is electrically connected with the optical path detection unit and is used for performing signal conversion on the optical signal output by the optical path detection unit and outputting a digital signal;
and the data processing unit is electrically connected with the signal conversion unit and used for analyzing and processing the digital signals output by the signal conversion unit and outputting analysis data.
2. The analytical system of claim 1, wherein the weak light detection optical path is configured to detect scattered light or fluorescence of the sample, and the transmitted light detection optical path is configured to detect transmitted light of the sample.
3. The analytical system of claim 1, wherein the detection cell in the detection light path comprises a detection zone and a capillary tube, the capillary tube is disposed in the detection zone, the capillary tube is a corrugated capillary tube, one end of the capillary tube is an inflow end, and the other end of the capillary tube is an outflow end.
4. The analytical system of claim 1, wherein the output optical signal of the weak light detection optical path is filtered by a first optical filter and sent to the photoelectric conversion unit, the first optical filter being mounted in a first circular filter wheel, the first circular filter wheel being driven by a first stepper motor.
5. The analysis system according to claim 1, wherein the sample processing unit comprises an original sample sampling path, a preprocessed sample sampling path, and at least one mixed sample sampling path, and samples respectively obtained through the original sample sampling path, the preprocessed sample sampling path, and the mixed sample sampling path are detected by the optical path detection unit.
6. The analytical system of claim 5, wherein the sample processing unit further comprises: the cleaning device comprises a cleaning passage and a compressed air passage, wherein the pretreatment sample sampling passage and the mixed sample sampling passage are respectively communicated with the cleaning passage, and the pretreatment sample sampling passage and the mixed sample sampling passage are respectively communicated with the compressed air passage.
7. The analysis system according to claim 1, wherein the sample processing unit includes an original sample sampling path and a multi-sample sampling path, and samples obtained through the original sample sampling path and the multi-sample sampling path, respectively, are detected by the optical path detection unit.
8. The analytical system of claim 7, wherein the multi-sample sampling path comprises a reaction disk provided with at least two reaction cups, and the sample in the reaction cup is detected by the optical path detection unit.
9. The analysis system of claim 8, wherein the detection light path for detecting the sample in the reaction cup comprises a detection module, and a detection groove matched with the reaction disk is formed in the detection module.
10. The analysis system according to claim 1, wherein the signal conversion unit includes a photoelectric conversion element that converts the optical signal output from the optical path detection unit into an electrical signal, and an analog-to-digital conversion element that converts the electrical signal into a digital signal.
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