CN113830892A - Device for synergistic denitrification of anaerobic ammonium oxidation bacteria coupled with heterotrophic denitrifying bacteria and culture method thereof - Google Patents
Device for synergistic denitrification of anaerobic ammonium oxidation bacteria coupled with heterotrophic denitrifying bacteria and culture method thereof Download PDFInfo
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- 230000003647 oxidation Effects 0.000 title claims abstract description 64
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 64
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- 238000012136 culture method Methods 0.000 title abstract description 7
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 18
- 230000001105 regulatory effect Effects 0.000 claims abstract description 12
- 230000000694 effects Effects 0.000 claims abstract description 10
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- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 9
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- 238000005070 sampling Methods 0.000 claims abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 22
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 11
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims description 8
- 230000008878 coupling Effects 0.000 claims description 8
- 238000010168 coupling process Methods 0.000 claims description 8
- 238000005859 coupling reaction Methods 0.000 claims description 8
- 239000006260 foam Substances 0.000 claims description 7
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 claims description 7
- 239000007789 gas Substances 0.000 claims description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 6
- 230000007704 transition Effects 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 3
- 239000003513 alkali Substances 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 239000004317 sodium nitrate Substances 0.000 claims description 3
- 235000010344 sodium nitrate Nutrition 0.000 claims description 3
- 235000010288 sodium nitrite Nutrition 0.000 claims description 3
- 238000004804 winding Methods 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 241000233866 Fungi Species 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 29
- 229910021529 ammonia Inorganic materials 0.000 description 15
- 239000010865 sewage Substances 0.000 description 14
- 230000008569 process Effects 0.000 description 12
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- 238000006243 chemical reaction Methods 0.000 description 6
- 239000000758 substrate Substances 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 230000010718 Oxidation Activity Effects 0.000 description 2
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 208000035404 Autolysis Diseases 0.000 description 1
- 101100314150 Caenorhabditis elegans tank-1 gene Proteins 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241001453382 Nitrosomonadales Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/282—Anaerobic digestion processes using anaerobic sequencing batch reactors
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2866—Particular arrangements for anaerobic reactors
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- Life Sciences & Earth Sciences (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to a device for the cooperative denitrification of anaerobic ammonium oxidation bacteria coupled with heterotrophic denitrifying bacteria and a culture method thereof, which comprises a regulating water tank, a reactor main body and a water outlet water tank; the regulating water tank is connected with the reactor main body through a water inlet pump; the reactor main body is connected with a water outlet tank through a water outlet pipe; the reactor main body is provided with a water inlet pipe, a dosing pump, a thermometer, a mechanical stirrer, an exhaust pipe, a water outlet pipe, a sampling port and a sludge discharge pipe; wherein the dosing pump and the mechanical stirrer are respectively connected with the timing device; the exhaust pipe is connected with the gas buffer bottle; a filter screen is arranged at the water outlet pipe. According to the device, the anaerobic ammonium oxidation bacteria are enriched, the adding amount of the nitrate and the carbon source in the raw water is increased gradually after the anaerobic ammonium oxidation bacteria are stabilized, and the denitrification activity in the anaerobic ammonium oxidation reactor is activated gradually, so that the inhibition of the anaerobic ammonium oxidation bacteria activity by the direct excessive carbon source and the excessive proliferation of the denitrification bacteria caused by adding the nitrate and the carbon source are avoided.
Description
Technical Field
The invention relates to the technical field of biological sewage treatment, and relates to a device for synergistic denitrification of anaerobic ammonium oxidation bacteria coupled with heterotrophic denitrifying bacteria and a culture method thereof.
Background
Excessive discharge of nitrogen can cause serious environmental damage. The traditional nitrification and denitrification biological process treatment process needs a large amount of aeration, so that the power cost is high. When the wastewater with low carbon-nitrogen ratio is treated, the treatment cost is increased due to the large addition of the carbon source. The anaerobic ammonia oxidation process is a novel autotrophic nitrogen removal process, does not consume an organic carbon source, and is mainly used for treating sewage with a low carbon-nitrogen ratio. The substrate necessary for the anammox process comprises nitrite, and stable production of nitrite requires that the anammox process be used in conjunction with other processes. The method is a feasible scheme for solving the problem that part of ammonia nitrogen in the sewage is nitrified into nitrate in the whole process, and then the residual ammonia nitrogen and the nitrate in the sewage are removed by the anaerobic ammonia oxidation and heterotrophic denitrification process.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides a device and a method for realizing the treatment of domestic sewage by anaerobic ammonia oxidation coupled heterotrophic denitrification, so that the problem of insufficient carbon source in the traditional denitrification process is solved, the process flow is simple, the operation cost is low, the sludge yield is low, the advantages of novel biological denitrification technologies such as anaerobic ammonia oxidation and denitrification are combined, the raw water carbon source is utilized to the maximum extent, and the high-efficiency and low-energy-consumption denitrification of the sewage is realized.
The invention aims to improve the denitrification efficiency of an anaerobic ammonia oxidation reactor, and provides an anaerobic ammonia oxidation coupling heterotrophic denitrification coordinated denitrification device and a culture method thereof for municipal sewage.
The technical scheme of the invention is as follows:
a device for the cooperative denitrification of anaerobic ammonium oxidation bacteria coupled with heterotrophic denitrifying bacteria comprises a regulating water tank, a reactor main body and a water outlet water tank; the regulating water tank is connected with the reactor main body through a water inlet pump; the reactor main body is connected with a water outlet tank through water outlet; the reactor main body is provided with a water inlet pipe, a dosing pump, a thermometer, a mechanical stirrer, an exhaust pipe, a water outlet pipe, a sampling port and a sludge discharge pipe; wherein the dosing pump and the mechanical stirrer are respectively connected with the timing device (12); the exhaust pipe is connected with the gas buffer bottle; a filter screen is arranged at the water outlet pipe; and a circulating water bath and a foam pad are arranged outside the reactor main body.
A method for the synergistic denitrification of anaerobic ammonium oxidation bacteria coupled with heterotrophic denitrifying bacteria comprises the following specific operation steps:
(1) enrichment culture stage of anammox bacteria:
the reactor operates in a semi-sequential batch mode, nitrogen is introduced into raw water for 30 minutes to remove dissolved oxygen in the inlet water, the dissolved oxygen in the inlet water is reduced to be below 0.5mg/L, the concentrations of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in the inlet water and the outlet water of the reactor are periodically measured, the enrichment culture condition of anaerobic ammonium oxidation bacteria in the anaerobic ammonium oxidation reactor is judged, the anaerobic ammonium oxidation sludge is reddish brown after enrichment,
(2) the anaerobic ammonium oxidation bacteria and heterotrophic denitrifying bacteria are used for the synergic denitrification stage:
after enriching the anaerobic ammonium oxidation bacteria in the reactor, gradually increasing the adding amount of nitrate and carbon source in raw water, gradually activating the denitrification activity in the anaerobic ammonium oxidation reactor, adopting sodium acetate as the carbon source, ammonium sulfate, sodium nitrate and sodium nitrite as the nitrogen source, dividing the operation of the anaerobic ammonium oxidation coupling heterotrophic denitrification reactor into three stages, namely a start-up stage, a transition stage and an operation stage, wherein the start-up stage is 1-7 days, and the water inlet composition of the anaerobic ammonium oxidation coupling heterotrophic denitrification reactor is 50-100mg/L,
The 8 th to 9 th days are transition period, the influent water composition is COD 100mg/L,
The 10 th day later is an operation stage, the water inlet composition is COD 110-,
Hydrochloric acid or alkali is adopted in the adjusting water tank to adjust the pH value to be 7.8-8.0, nitrogen is introduced for 30 minutes to remove dissolved oxygen in the inlet water, and the dissolved oxygen in the inlet water is reduced to be below 0.5 mg/L.
The timing device controls the operation period of the reactor, the water feeding and stirring are synchronously carried out for 11.5 hours, and the precipitation and the water drainage are carried out for 15min respectively in sequence to form a period.
The circulating water bath was formed by tightly winding a plastic hose into which hot water was introduced so that the temperature measured by a thermometer was 35. + -. 1 ℃.
The foam pad is arranged outside the circulating water bath, and has the functions of heat preservation and light protection, so that an environment suitable for growth of the anaerobic ammonia oxidizing bacteria is created.
The rotating speed of the mechanical stirrer is 140rad/min, and the function of the mechanical stirrer is to accelerate mass transfer of microorganisms.
The sewage treatment process in the device comprises the following steps: the urban sewage is pumped into the reactor main body by the regulating water tank through the water inlet pump; in the reactor main body, anaerobic ammonium oxidation bacteria are enriched and cultured, ammonia nitrogen and nitrite nitrogen are added, the concentration ratio of the ammonia nitrogen to the nitrite nitrogen is controlled to be about 1:1.32, most of aerobic bacteria and heterotrophic bacteria die after a thallus autolysis stage and an activity retardation stage, and the anaerobic ammonium oxidation sludge after enrichment is reddish brown; then, in the reactor main body, the adding amount of nitrate and carbon source in the inlet water is gradually increased, the denitrification activity in the anaerobic ammonia oxidation reactor is gradually activated, and denitrifying bacteria use anaerobic ammoniaNitrate produced in the oxidation process and a low-concentration carbon source are used as substrates to proliferate, on the one hand, anaerobic ammonium oxidation bacteria proliferate in the reactor body
And
conversion to N2And small amount
On the other hand, the denitrifying bacteria utilize the organic matters in the reactor body to oxidize the anaerobic ammonia
And the rest of
Conversion to N2And the effluent is discharged to the effluent water tank through the water outlet pipe (9), so that the urban domestic sewage treatment is realized.
Compared with the prior art, the invention has the following beneficial effects:
firstly, in China, anaerobic ammonia oxidation and denitrification are coupled to the same reactor, the reactor is unstable in operation and low in impact load resistance, and the flora in the reactor can be completely replaced by denitrifying bacteria; secondly, compared with the sequencing batch type operation mode which is researched more at present, the semi-sequencing batch type reactor can reduce the inhibiting effect of high influent carbon source concentration on the anaerobic ammonia oxidation activity in the anaerobic ammonia oxidation coupling denitrification system, in the sequencing batch type reactor, the substrate is added at one time, and the anaerobic ammonia oxidation bacteria and the heterotrophic denitrifying bacteria have NO inhibition effect on the electron acceptor in the reaction system2 -The competition cannot be controlled, and the semi-sequence batch operationIn a running mode, the input of the substrate can be artificially controlled, the substrate is at a lower concentration in the reaction system, and the influence of high carbon source concentration on the anaerobic ammonia oxidation activity is partially eliminated.
Drawings
FIG. 1 is a schematic structural diagram of an apparatus for the synergistic denitrification of anammox bacteria coupled with heterotrophic denitrifying bacteria according to the present invention;
in the figure: the device comprises a regulating water tank-1, a reactor main body-2, a water outlet tank-3, a water inlet pump-4, a dosing pump-5, a water inlet pipe-6, a dosing pipe-7, an exhaust pipe-8, a water outlet pipe-9, a sampling port-10, a sludge discharge pipe-11, a timing device-12, a filter screen-13, a circulating water bath-14, a foam pad-15, a gas buffer bottle-16, a thermometer-17 and a mechanical stirrer-18.
Detailed Description
The invention is further described in the following with reference to the following figures and examples:
as shown in fig. 1, the device for the synergistic denitrification of the anaerobic ammonium oxidation bacteria coupled with the heterotrophic denitrifying bacteria and the culture method thereof are characterized by comprising a regulating water tank 1, a reactor main body 2 and an effluent water tank 3; wherein the regulating water tank 1 is connected with the reactor main body 2 through a water inlet pump 4; the reactor body 2 is connected with the water outlet tank 3 through a water outlet pipe 9. The reactor main body 2 is provided with a water inlet pipe 6, a dosing pipe 7, a dosing pump 5, a thermometer 17, a mechanical stirrer 18, an exhaust pipe 8, a water outlet pipe 9, a sampling port 10 and a sludge discharge pipe 11; wherein the dosing pump 5 and the mechanical stirrer 18 are respectively connected with the timing device 12; the exhaust pipe 8 is connected with a gas buffer bottle 16; a filter screen 13 is arranged at the water outlet pipe 9. The reactor main body 2 is externally provided with a circulating water bath 14 and a foam pad 15.
The specific operation is as follows:
1 enrichment culture stage of anaerobic ammonium oxidation bacteria:
the reactor is operated in a semi-sequential batch mode, nitrogen is introduced into raw water for 30 minutes to remove dissolved oxygen in the inlet water, and the dissolved oxygen in the inlet water is reduced to be below 0.5 mg/L. And (3) periodically measuring the concentrations of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in the inlet water and the outlet water of the reactor, and judging the enrichment culture condition of anaerobic ammonium oxidation bacteria in the anaerobic ammonium oxidation reactor, wherein the enriched anaerobic ammonium oxidation sludge is reddish brown.
2, a coordinated denitrification stage of anammox bacteria and heterotrophic denitrifying bacteria:
after the anaerobic ammonium oxidation bacteria are enriched in the reactor, the adding amount of nitrate and carbon source in raw water is gradually increased, and the denitrification activity in the anaerobic ammonium oxidation reactor is gradually activated. Sodium acetate is used as a carbon source, and ammonium sulfate, sodium nitrate and sodium nitrite are used as nitrogen sources. The operation of the anaerobic ammonia oxidation coupled heterotrophic denitrification reactor is divided into three stages, namely a start-up stage, a transition stage and an operation stage. The 1 st to 7 th days are the starting period, the water inlet composition is COD 50-100mg/L,
The 8 th to 9 th days are transition period, the influent water composition is COD 100mg/L,
The 10 th day later is an operation stage, the water inlet composition is COD 110-,
Further, hydrochloric acid or alkali is adopted in the adjusting water tank 1 to adjust the pH value to be 7.8-8.0, nitrogen is introduced for 30 minutes to remove dissolved oxygen in the inlet water, and the dissolved oxygen in the inlet water is reduced to be below 0.5 mg/L.
Further, the timing device 12 controls the operation cycle of the reactor, the water feeding and stirring are synchronously carried out for 11.5 hours, and the precipitation and the water drainage are carried out for 15min respectively in sequence to form a cycle.
Further, the circulating water bath 14 is formed by tightly winding a plastic hose, and hot water is introduced into the hose so that the temperature measured by the thermometer 17 is 35. + -. 1 ℃.
Further, the foam pad 15 is disposed outside the circulating water bath 14, and functions to keep warm and light away, creating an environment suitable for the growth of anammox bacteria.
Further, the rotation speed of the mechanical stirrer 18 is 140rad/min, and the function of the mechanical stirrer is to accelerate the mass transfer of the microorganisms.
The water inlet adopts a semi-sequential batch input mode, namely the water inlet and the stirring are synchronously carried out for 11.5h, the precipitation and the water drainage are carried out for 15min in sequence respectively to form a period, the gas generated by the reaction is discharged to a gas buffer bottle 16 from an exhaust pipe 8 at the top of the reactor, and the outlet water flows out to an outlet water tank 3 from a water outlet pipe 9. The speed of the mechanical stirrer 18 is 140 rad/min. The culture temperature is controlled at 35 +/-1 ℃, and the pH value of the test inlet water is between 7.8 and 8.0. The pH value of the inlet water is adjusted by hydrochloric acid or caustic soda, and the culture temperature is controlled by circulating water bath. The formula for culturing the anaerobic ammonium oxidation bacteria is as follows: the NH42SO4And NaNO2The concentration of the ammonia nitrogen and the nitrite nitrogen is configured as required, and the concentration ratio of the ammonia nitrogen to the nitrite nitrogen is controlled to be about 1: 1.32; other materials include NaHCO at a concentration of 1.0g/L3KH of 0.021g/L2PO4CaCl with concentration of 0.14g/L2MgSO 0.166g/L MgSO4The concentration of the trace element I is 1mL/L, the concentration of the trace element II is 1mL/L, and the concentration of the trace element I is 5g/L of FeSO4EDTA with the concentration of 5 g/L; the microelements II are EDTA with the concentration of 15g/L and ZnSO with the concentration of 0.43g/L4·H2O, CoCl with concentration of 0.24g/L2·6H2O, CuSO with concentration of 0.25g/L4·5H2O, concentration 0.22g/LNaMoO4·2H2O, NiCl with a concentration of 0.19g/L2·5H2O, concentration 0.014g/LH3BO4The concentration of the sodium hydroxide is 0.05g/LNaWO4·2H2O。
In summary, the following steps: the invention belongs to the technical field of biological sewage treatment, and relates to a device for the synergistic denitrification of anaerobic ammonium oxidation bacteria coupled with heterotrophic denitrifying bacteria and a culture method thereof. The device comprises a regulating water tank, an anaerobic ammonia oxidation coupling heterotrophic denitrification reactor main body and a water outlet water tank. The method comprises the steps of feeding urban domestic sewage into a regulating water tank, controlling the concentration ratio of ammonia nitrogen to nitrite nitrogen in the fed water to be about 1:1.32 through real-time control, feeding the sewage into a reactor main body, and enriching and domesticating anaerobic ammonium oxidation bacteria in the reactor. After the anaerobic ammonium oxidation bacteria are enriched in the reactor, the adding amount of nitrate and a carbon source in raw water is gradually increased, the denitrification activity in the anaerobic ammonium oxidation reactor is gradually activated, the synergistic reaction of anaerobic ammonium oxidation and heterotrophic denitrification is simultaneously realized in the reactor, and the denitrification effect is achieved. The invention improves the denitrification efficiency by fully utilizing the synergistic effect among microorganisms, and realizes the high-efficiency and energy-saving deep denitrification of the urban domestic sewage.
Claims (7)
1. The utility model provides a device of anaerobic ammonium oxidation fungus coupling heterotrophic denitrifying bacterium denitrogenation in coordination which characterized in that: comprises a regulating water tank (1), a reactor main body (2) and a water outlet water tank (3); wherein the regulating water tank (1) is connected with the reactor main body (2) through a water inlet pump (4); the reactor main body (2) is connected with the water outlet tank (3) through a water outlet pipe (9); the reactor main body (2) is provided with a water inlet pipe (6), a dosing pipe (7), a dosing pump (5), a thermometer (17), a mechanical stirrer (18), an exhaust pipe (8), a water outlet pipe (9), a sampling port (10) and a sludge discharge pipe (11); wherein the dosing pump (5) and the mechanical stirrer (18) are respectively connected with the timing device (12); the exhaust pipe (8) is connected with the gas buffer bottle (16); a filter screen (13) is arranged at the water outlet pipe (9); and a circulating water bath (14) and a foam pad (15) are arranged outside the reactor main body (2).
2. The method for culturing the anaerobic ammonium oxidation bacteria coupled with the heterotrophic denitrifying bacteria for the synergic denitrification of the anaerobic ammonium oxidation bacteria as claimed in claim 1, wherein: the specific operation steps are as follows:
(1) enrichment culture stage of anammox bacteria:
the reactor operates in a semi-sequential batch mode, nitrogen is introduced into raw water for 30 minutes to remove dissolved oxygen in the inlet water, the dissolved oxygen in the inlet water is reduced to be below 0.5mg/L, the concentrations of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in the inlet water and the outlet water of the reactor are periodically measured, the enrichment culture condition of anaerobic ammonium oxidation bacteria in the anaerobic ammonium oxidation reactor is judged, the anaerobic ammonium oxidation sludge is reddish brown after enrichment,
(2) the anaerobic ammonium oxidation bacteria and heterotrophic denitrifying bacteria are used for the synergic denitrification stage:
after enriching the anaerobic ammonium oxidation bacteria in the reactor, gradually increasing the adding amount of nitrate and carbon source in raw water, gradually activating the denitrification activity in the anaerobic ammonium oxidation reactor, adopting sodium acetate as the carbon source, ammonium sulfate, sodium nitrate and sodium nitrite as the nitrogen source, dividing the operation of the anaerobic ammonium oxidation coupling heterotrophic denitrification reactor into three stages, namely a start-up stage, a transition stage and an operation stage, wherein the start-up stage is 1-7 days, and the water inlet composition of the anaerobic ammonium oxidation coupling heterotrophic denitrification reactor is 50-100mg/L,
The 8 th to 9 th days are transition period, the influent water composition is COD 100mg/L,
The 10 th day later is an operation stage, the water inlet composition is COD 110-,
3. The method for the synergistic denitrification of the anaerobic ammonium oxidation bacteria coupled with the heterotrophic denitrifying bacteria, which is disclosed by the claim 2, is characterized in that: hydrochloric acid or alkali is adopted in the adjusting water tank (1) to adjust the pH value to be 7.8-8.0, nitrogen is introduced for 30 minutes to remove dissolved oxygen in the inlet water, and the dissolved oxygen in the inlet water is reduced to be below 0.5 mg/L.
4. The method for the synergistic denitrification of the anaerobic ammonium oxidation bacteria coupled with the heterotrophic denitrifying bacteria, which is disclosed by the claim 2, is characterized in that: the timing device (12) controls the operation period of the reactor, water feeding and stirring are synchronously carried out for 11.5 hours, and precipitation and water drainage are carried out for 15min respectively in sequence to form a period.
5. The method for the synergistic denitrification of the anaerobic ammonium oxidation bacteria coupled with the heterotrophic denitrifying bacteria, which is disclosed by the claim 2, is characterized in that: the circulating water bath (14) is formed by tightly winding a plastic hose, and hot water is introduced into the hose, so that the temperature measured by the thermometer (17) is 35 +/-1 ℃.
6. The method for the synergistic denitrification of the anaerobic ammonium oxidation bacteria coupled with the heterotrophic denitrifying bacteria, which is disclosed by the claim 2, is characterized in that: the foam pad (15) is arranged outside the circulating water bath (14) and has the functions of heat preservation and light protection, so that an environment suitable for growth of anaerobic ammonium oxidation bacteria is created.
7. The method for the synergistic denitrification of the anaerobic ammonium oxidation bacteria coupled with the heterotrophic denitrifying bacteria, which is disclosed by the claim 2, is characterized in that: the rotating speed of the mechanical stirrer (18) is 140rad/min, and the function of the mechanical stirrer is to accelerate the mass transfer of microorganisms.
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Application publication date: 20211224 |