CN113813076B - Skull positioning device for hypothalamus region of mouse - Google Patents
Skull positioning device for hypothalamus region of mouse Download PDFInfo
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- CN113813076B CN113813076B CN202111123097.1A CN202111123097A CN113813076B CN 113813076 B CN113813076 B CN 113813076B CN 202111123097 A CN202111123097 A CN 202111123097A CN 113813076 B CN113813076 B CN 113813076B
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- 210000003625 skull Anatomy 0.000 title claims abstract description 33
- 210000003016 hypothalamus Anatomy 0.000 title claims abstract description 21
- 230000007246 mechanism Effects 0.000 claims abstract description 25
- 230000002267 hypothalamic effect Effects 0.000 claims abstract description 14
- 210000001015 abdomen Anatomy 0.000 claims description 9
- 239000003550 marker Substances 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims description 5
- 210000000216 zygoma Anatomy 0.000 claims description 3
- 238000002347 injection Methods 0.000 abstract description 5
- 239000007924 injection Substances 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 101
- 210000004373 mandible Anatomy 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 9
- 238000007906 compression Methods 0.000 description 8
- 230000006835 compression Effects 0.000 description 7
- 241000700159 Rattus Species 0.000 description 3
- 230000003187 abdominal effect Effects 0.000 description 3
- 229910052736 halogen Inorganic materials 0.000 description 3
- 150000002367 halogens Chemical class 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 125000001475 halogen functional group Chemical group 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
- A61D2003/003—Appliances for supporting or fettering animals for operative purposes with head or neck restraining means
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dental Tools And Instruments Or Auxiliary Dental Instruments (AREA)
- Surgical Instruments (AREA)
Abstract
The invention provides a skull positioning device for a hypothalamic area of a mouse, which comprises a base for fixing the mouse and a mouse head positioning mechanism for positioning and clamping the head of the mouse; the mouse head positioning mechanism comprises a side positioner for fixing the skull of the cheek surfaces at two sides of the mouse and a second pressing sheet for fixing the top of the mouse head; a platform is supported on the base through a bracket, and a driving mechanism is arranged on the platform and used for driving the side positioner and the second pressing piece to position and clamp the head of the mouse; in the mouse hypothalamus skull positioning device, the lateral positioner is used for positioning and clamping the skull on two sides of the mouse, and the second pressing sheet is used for positioning and clamping the head and the top of the mouse, so that the head of the mouse is not easy to flick when the mouse is stressed, and the hypothalamus medicine injection and other operations are convenient to perform.
Description
Technical Field
The invention relates to a group of medical experimental instruments, in particular to a skull positioning device for a hypothalamic area of a mouse.
Background
Mice are common subjects in biological experiments, and in order to study the hypothalamus of mice, the hypothalamus of mice is sometimes subjected to injection of drugs by a syringe or resection.
In general, in such experiments, since mice are living animals, most of them have stress reaction in the face of the experiment, and thus, after placing the mice on an experimental instrument, the heads of the mice need to be fixed; the existing fixing device is simple in structure, most of the fixing device only presses the head of a mouse, so that when the mouse is subjected to stress reaction, the head is easy to move left and right, and hypothalamus experimental operation cannot be accurately performed.
In view of this, the present invention provides an improvement in the manner of fixing mice.
Disclosure of Invention
The invention aims to overcome the defects of the background technology, provides a skull positioning device for a mouse hypothalamus region, and particularly provides a novel positioning device for positioning and clamping the skull of two sides of the cheek surface of a mouse, so that the shaking of the mouse during stress is reduced, and the accuracy and the convenience of experiments are improved.
The embodiment of the invention is realized by the following technical scheme:
the skull positioning device for the hypothalamus area of the mouse comprises a base for fixing the mouse and a mouse head positioning mechanism for positioning and clamping the head of the mouse; the mouse head positioning mechanism comprises a side positioner for fixing the skull of the cheek surfaces at two sides of the mouse and a second pressing sheet for fixing the top of the mouse head;
a platform is supported on the base through a support, and a driving mechanism is arranged on the platform and used for driving the side positioner and the second pressing piece to position and clamp the head of the mouse.
Preferably, the driving mechanism comprises an outer cylinder vertically arranged on the platform and an inner cylinder which is arranged in the outer cylinder in a vertically sliding manner, the inner cylinder is used for accommodating the injector, and a third elastic piece is arranged between the outer cylinder and the inner cylinder and used for driving the inner cylinder to move upwards; the lower end of the inner cylinder body is provided with an extension cylinder, the extension cylinder penetrates through and extends to the lower part of the outer cylinder body, and the second pressing sheet is arranged at the lower end part of the extension cylinder, so that the needle head of the injector can penetrate into the head of the mouse through the extension cylinder;
the side locator comprises two groups of first pressing sheets which are oppositely arranged and are used for pressing the skull of the cheek surfaces at two sides of the mouse; the side positioner further comprises two sets of threaded pipes which are oppositely arranged, each set of threaded pipes is connected with a second screw rod, a movable piece is slidably arranged in one end of the head of the mouse, the first end of the movable piece is abutted against the second screw rod, the second end of the movable piece is connected with the first pressing piece through a set of compressed first elastic pieces, so that the movable piece and the first elastic pieces are pushed by screwing the second screw rods, the first pressing piece extrudes the cheek bones on two sides of the mouse, and a set of extrusion pieces with gaps are reserved between the second end of the movable piece and the first pressing piece;
two groups of vertical rods are further arranged on the inner cylinder body, a wedge-shaped part is arranged at the lower end of each group of vertical rods, the surface of a threaded pipe right below the wedge-shaped part is provided with a through hole, inclined planes are arranged on the abutting end surfaces of a second screw rod in each group of threaded pipes and the movable piece, so that an upward horn-shaped opening is formed between the second screw rod and the movable piece, and the through hole is positioned above the opening;
when the injector is inserted into the inner cylinder body to enable the inner cylinder body to move downwards, the second pressing piece presses the head top of the mouse, and the wedge-shaped parts at the lower ends of the vertical rods of each group are driven to be inserted into the opening to enable the movable piece to move so as to press the first pressing piece through the pressing piece.
Preferably, the movement travel of the second screw in the threaded tube is not greater than the maximum width of the opening, so that the opening is always located below the through hole.
Preferably, the mouse mandible fixing device further comprises a mandible positioner for fixing the mouse mandible, wherein the mandible positioner comprises a second sliding block which is axially movably arranged on the base along the screw rod, and the second sliding block is connected with the base through a group of third screws so that the second sliding block can be fixed at any position of the self moving stroke;
the second sliding block is also provided with a supporting block for supporting the mouse mandible, and the surface of the second sliding block contacted with the mouse mandible is an arc-shaped surface matched with the contour of the mouse mandible.
Preferably, the second sliding block is further provided with a marker post for positioning the mouse front marinating point, and the marker post is provided with a graduated scale for displaying the distance between the mouse hypothalamus and the front marinating point in the length direction of the screw rod.
Preferably, the mouse mouth fixing device further comprises a mouse mouth positioner for fixing the mouse mouth, wherein the mouse mouth positioner is used for being sleeved on the mouse mouth, and the mouse mouth positioner is arranged on the second sliding block.
Preferably, the mouse mouth positioner comprises a circular ring arranged on the second sliding block, a plurality of cap shells are arranged on the outer wall of the circular ring in a circumferential array, pistons are arranged in the cap shells, each group of cap shells is connected with the piston through a stretching-shaped second elastic piece, the tail end of each piston is connected with a third pressing sheet positioned in the circular ring through two groups of guide rods, and the third pressing sheets are arranged around the axis of the circular ring;
the outer walls of the plurality of third pressing sheets are sleeved with a group of ropes, the ropes sleeved on the outer walls of the plurality of third pressing sheets penetrate through the two groups of guide rods, the tail ends of the ropes penetrate through the circular rings and are sleeved on the support, the plurality of third pressing sheets are sleeved on the mouse mouth, and the second sliding blocks move towards the mouse mouth to enable the ropes to be in a stretching shape;
the inner cylinder body is also provided with a compression bar which is in compression joint with the rope, and when the inner cylinder body moves downwards, the compression bar is pressed downwards to enable the rope to tighten the plurality of third pressing pieces so as to fasten the mouse mouth.
Preferably, a screw pair is arranged on a bracket of the base, the screw pair comprises a screw rod rotatably arranged on the bracket and a first sliding block arranged on the screw rod, the screw rod is horizontally arranged, a guide piece is horizontally arranged on the bracket, and the first sliding block is slidably arranged on the guide piece in a penetrating manner so that the first sliding block can horizontally reciprocate along the length direction of the screw rod;
the guide piece is also slidably provided with the platform, the platform is in driving connection with the first sliding block, so that the first sliding block drives the platform to synchronously move, and the driving mechanism and the side surface positioner are arranged on the platform.
Preferably, the abdomen clamp for fixing the waist of the mouse is further arranged, the upper end of the abdomen clamp is connected with a first screw rod which is vertically arranged, the upper end of the first screw rod is in threaded connection with the first sliding block, and the abdomen clamp is enabled to move downwards through screwing the first screw rod so as to press the abdomen of the mouse on the base.
Preferably, the first screw rod vertically penetrates through the platform, and the first sliding block is in driving connection with the platform through the first screw rod.
The technical scheme of the embodiment of the invention has at least the following advantages and beneficial effects:
in the mouse hypothalamus skull positioning device, the lateral positioner is used for positioning and clamping the skull on two sides of the mouse, and the second pressing sheet is used for positioning and clamping the head and the top of the mouse, so that the head of the mouse is not easy to flick when the mouse is stressed, and the hypothalamus medicine injection and other operations are convenient to perform.
Drawings
FIG. 1 is a schematic plan view of a mouse hypothalamic skull positioning device of the present invention;
FIG. 2 is an enlarged view of portion A of FIG. 1;
FIG. 3 is a schematic diagram of a rat head positioning mechanism in the mouse hypothalamic skull positioning device of the present invention;
FIG. 4 is a perspective view of a mouse mouth locator in a mouse hypothalamic skull positioning device of the present invention;
FIG. 5 is a schematic diagram of the operation of the mouse mouth locator of the present invention;
icon: 1-base, 10-bracket, 10 a-cross frame, 11-screw pair, 11 a-screw, 11 b-first slider, 11 c-guide, 12-platform, 12 a-first connector, 13-chute, 2-mouse head positioning mechanism, 20-first screw, 20 a-abdominal clamp, 21-side positioner, 21 a-threaded tube, 21 b-second screw, 21 c-movable member, 21 d-extrusion, 21 e-first tab, 21 f-first elastic member, 22-second tab, 23-mouse mouth positioner, 23 a-ring, 23 b-cap, 23 c-rope, 23 d-third tab, 23 e-extension, 23 f-guide rod, 23 g-second elastic piece, 23 h-piston, 3-driving mechanism, 30-inner cylinder, 30 a-extending ear plate, 30 b-holding die, 30 c-needle cavity, 30 d-limit ring, 30 e-extending cylinder, 31-outer cylinder, 32-third elastic piece, 33-vertical rod, 33 a-wedge part, 34-second connecting piece, 35-third connecting piece, 35 a-compression rod, 4-second slide block, 40-supporting block, 41-third screw rod, 42-mark post, 420-scale, 5-mouse, 5 a-front halogen point, 5 b-hypothalamus, 6-syringe, 60-needle.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Referring to fig. 1-5, the present invention provides a skull positioning device for the hypothalamus 5b region of the head of a small murine species.
The device comprises a base 1 for fixing a mouse 5 and a mouse head positioning mechanism 2 for positioning and clamping the head of the mouse 5; the mouse head positioning mechanism 2 comprises a side positioner 21 for fixing the skull of the two sides of the cheek face of the mouse 5, a second pressing sheet 22 for fixing the top of the head of the mouse 5, a mandible positioner for fixing the mandible of the mouse and a mouse mouth positioner 23 for positioning and clamping the mouth of the mouse.
Wherein, a platform 12 is supported on the base 1 through a bracket 10, and a driving mechanism 3 is arranged on the platform 12 for driving a side positioner 21 and the second pressing piece 22 to position and clamp the head of the mouse 5.
Referring to fig. 1, in the embodiment, a screw pair 11 is provided on the bracket 10 of the base 1, the screw pair 11 includes a screw 11a rotatably provided on the bracket 10 and a first slider 11b provided on the screw 11a, the screw 11a extends along the length direction of the base 1, and the mouse 5 is placed and fixed along the length direction of the screw 11 a; the screw rod 11a is horizontally arranged, the support 10 is also horizontally provided with a guide piece 11c, the guide piece 11a can adopt two groups of parallel long rods or a group of square shafts, and the first sliding block 11b can be slidably arranged on the guide piece 11c in a penetrating manner so that the first sliding block 11b can horizontally reciprocate along the length direction of the screw rod 11 a.
The platform 12 is in driving connection with the first sliding block 11b, so that the first sliding block 11b drives the platform 12 to synchronously move, and the driving mechanism 3 and the side positioner 21 are arranged on the platform 12; specifically, the first slider 11b is threaded with a set of first screws 20, the lower ends of the first screws 20 are disposed on the platform 12 in a penetrating manner, the platform 12 is slidably connected with the guide piece 11c through the first connecting piece 12a, that is, the platform 12 is suspended on the guide piece 11c through a plurality of first connecting pieces 12a, the rotating wheel at the right end of the screw rod 11a in the screw rod pair 11 is rotated, and the first slider 11b moves and drives the platform 12 to move synchronously through the first screws 20.
The driving mechanism 3 is arranged on the upper end face of the platform 12, the side locator 21 is suspended and fixed below the platform 12 through the second connecting piece 34, and the screw pair 11 can drive the driving mechanism 3, the side locator 21 and the second pressing piece to synchronously move, so that mice with different sizes and placing positions can be conveniently positioned and clamped.
Referring to fig. 1 to 3, the driving mechanism 3 includes an outer cylinder 31 vertically disposed on the platform 12 and an inner cylinder 30 slidably disposed in the outer cylinder 31, the inner cylinder 30 is configured to accommodate the syringe 6, a third elastic member 32 is disposed between the outer cylinder 31 and the inner cylinder 30, and in this embodiment, the third elastic member 32 is disposed on a spring on the inner cylinder 30, an extending lug 30a is disposed on an outer side wall of an upper end of the inner cylinder 30, and the spring abuts between the extending lug 30a and an inner bottom of the outer cylinder 31 to drive the inner cylinder 30 to move upwards.
The lower end of the inner cylinder 30 is provided with a thinner extension cylinder 30e, the extension cylinder 30e extends to the lower part of the outer cylinder 31 in a penetrating way, and the second pressing piece 22 is arranged at the lower end part of the extension cylinder 30e, so that the needle 60 of the syringe 6 can penetrate into the head of the mouse 5 through the extension cylinder 30 e.
The outer side wall of the lower end of the inner cylinder 30 is provided with a limiting ring 30d to prevent the third elastic member 32 from ejecting the inner cylinder 30 from the outer cylinder 31.
The inner bottom of the inner cylinder 30 is provided with a holding die 30b adapted to the syringe 6, and the center of the holding die 30b is provided with a needle cavity 30c, which is convenient for the alignment of the needle 60.
Referring to fig. 3, the side locator 21 includes two sets of first pressing pieces 21e disposed opposite to each other for pressing the cheek bones on both sides of the mouse 5; the side positioner 21 further comprises two sets of threaded pipes 21a which are oppositely arranged, each set of threaded pipes 21a is connected with a second screw rod 21b, each set of threaded pipes 21a is further provided with a movable piece 21c in a sliding manner towards the inside of one end of the head of the mouse 5, the first end of each movable piece 21c is abutted to the corresponding second screw rod 21b, the second end of each movable piece is connected with the corresponding first pressing piece 21e through a set of compressed first elastic pieces 21f, when the initial positioning and clamping are carried out on the cheek face skull of the mouse 5, the second screw rods 21b on two sides are screwed, the second screw rods 21b are enabled to press the movable pieces 21c, the movable pieces 21c push the corresponding first elastic pieces 21f, and the first elastic pieces 21f push the corresponding first pressing pieces 21e to press the cheek face skull of the mouse.
In addition, in this embodiment, the first elastic member 21f is a spring, the second end of the movable member 21c is further provided with a set of pressing members 21d with a gap between the pressing members 21e, that is, the pressing members 21d are inserted into the spring, and when the skull of the cheek face of the mouse 5 is initially positioned and clamped, the right end of the pressing members 21d is not in contact with the first pressing members 21 e.
Referring to fig. 3, two sets of vertical rods 33 are further provided at the lower end of the extending ear plate 30a of the inner cylinder 30, the two sets of vertical rods 33 penetrate through the platform 12, and a wedge-shaped portion 33a is provided at the lower end of each set of vertical rods 33,
the surface of the threaded tube 21a right below the wedge-shaped portion 33a is provided with a through hole, and inclined surfaces are respectively arranged on the abutting end surfaces of the second screw rod 21b in each group of threaded tubes 21a and the movable piece 21c, so that an upward horn-shaped opening is formed between the second screw rod 21b and the movable piece 21c, and the through hole is positioned above the opening.
When hypothalamus 5b is injected, the injector 6 is inserted into the inner cylinder 30 to enable the inner cylinder 30 to move downwards, the second pressing piece 22 presses the top of the head of the mouse 5, the mouse 5 cannot shake upwards, the inner cylinder 30 moves downwards and drives the wedge-shaped parts 33a at the lower end of each group of vertical rods 33 to be inserted into the openings to enable the movable piece 21c to move towards the head of the mouse 5, at the moment, the pressing piece 21d presses the first pressing piece 21e, namely the pressing piece 21d replaces the first elastic piece 21f to be in rigid contact with the first pressing piece 21e, and compared with the first elastic piece 21f which is in contact with the first pressing piece 21e, the mouse 5 cannot shake left and right due to the contact of the pressing piece 21d, so that the injector 6 can be injected accurately.
In addition, when the needle 60 with a fixed length is adopted, the length of the needle 60 exposed out of the extension cylinder 30e is fixed, and when the second pressing sheet 22 presses the top of the mouse head, the needle 60 is pricked into the mouse head to a certain depth and just pricked into the hypothalamus 5b area, so that the driving mechanism 3 can also play a role in limiting and longitudinally positioning the pricking depth of the needle 60.
In some embodiments, the travel of the second screw 21b in the threaded tube 21a is not greater than the maximum width of the opening, so that the opening is always located below the through hole of the threaded tube 21a, facilitating the precise insertion and withdrawal of the wedge 33 a.
After the injection is completed, the syringe 6 is withdrawn and the third elastic member 32 urges the drive mechanism 3 to return, and the wedge portion 33a moves up to the outside of the threaded tube 21 a.
In addition, in some embodiments, the mouse fixing device further comprises a mandibular positioner for fixing the mandible of the mouse 5, referring to fig. 2, a sliding groove 3 is arranged on the base 1, and a sliding block 4 capable of sliding along the axial direction of the screw rod 11a is arranged in the sliding groove 3, specifically: the mandibular positioner comprises a second sliding block 4 which is axially movably arranged on the base 1 along the screw rod 11a, the second sliding block 4 is connected with the base 1 through a group of third screw rods 41, and a rotating wheel on the right side of the third screw rods 41 is screwed, so that the second sliding block 4 can be fixed at any position of the self moving stroke.
The mandibular fixator is arranged, so that the head of the mouse 5 can be kept horizontal, and the experimental accuracy is improved.
Furthermore, it is possible to provide a device for the treatment of a disease. The second sliding block 4 is also provided with a supporting block 40 for supporting the lower jaw of the mouse 5, the surface of the second sliding block 4 contacted with the lower jaw of the mouse 5 is an arc-shaped surface matched with the outline of the lower jaw of the mouse 5, and the head of the mouse 5 with different sizes can be adapted through the movement of the second sliding block 4.
In addition, the second slider 4 is further provided with a marker post 42 for positioning the front marinating point 5a of the mouse 5, and the marker post 42 is provided with a graduated scale 420 for displaying the distance between the hypothalamus 5b of the mouse 5 and the front marinating point 5a in the length direction of the screw rod 11 a; in general, in the industry, the rat hypothalamus 5b is located by the pre-halo 5a, and the interval parameters between the pre-halo 5a and the hypothalamus 5b of rats 5 of different individual sizes can be referred to from the existing literature; at this time, the lead screw 11a can be rotated to drive the platform 12 to move so that the marking on the second connector 34 is aligned with the spacing parameter scale on the scale 420 according to the size of the spacing parameter between the front halogen point 5a to the hypothalamus 5b of the mice 5 of different individual sizes by aligning the marking post 42 with the front halogen point 5a, while the extension cylinder 30e is aligned with the hypothalamus 5b.
In some preferred embodiments, the mouse mouth positioner 23 for fixing the mouse 5 mouth is further included, the mouse mouth positioner 23 is used for sleeving the mouse 5 mouth, and the mouse mouth positioner 23 is arranged on the second sliding block 4, and the mouse mouth positioner 23 can perform the preliminary positioning and limiting functions on the mouse 5 mouth before injection.
Because the mouth is easy to emit sound and is continuously opened and closed when the mouse 5 is stressed, and a certain head is rocked, referring to fig. 2, 4 and 5, in some embodiments, the mouse mouth positioner 23 comprises a circular ring 23a mounted on the second slider 4, a plurality of cap shells 23b are arranged on the outer wall of the circular ring 23a in a circumferential array manner, a piston 23h is arranged in each cap shell 23b, the piston 23h is connected with the interior of each cap shell 23b through a stretched second elastic piece 23g, the tail end of the piston 23h is connected with a third pressing piece 23d positioned in the circular ring 23a through two groups of guide rods 23f, and the third pressing pieces 23d are arranged around the axis of the circular ring 23 a;
namely, the third pressing pieces 23d can stretch and retract in the cap shell 23b, and under the action of the second elastic piece 23g, the third pressing pieces 23d can shrink towards the cap shell 23b, when the second elastic piece 23g is in a natural state, the mouse mouth stretches into the middle of the ring formed by the third pressing pieces 23d, and the second elastic piece 23g is slightly compressed to form primary clamping of the mouse mouth.
Referring to fig. 4, the outer walls of the third pressing pieces 23d are sleeved by a set of ropes 23c, and the ropes 23c sleeved on the outer walls of the third pressing pieces 23d are threaded between two sets of guide rods 23f, that is, the ropes 23c cannot be separated from the outer walls of the third pressing pieces 23 d.
Referring to fig. 2, the support 10 is further provided with a horizontal transverse frame 10a, the end of the other end of the rope 23c passes through the circular ring 23a and is sleeved on the transverse frame 10a, and the plurality of third pressing pieces 23d are sleeved on the mouth of the mouse.
The second slider 4 is moved to the left in fig. 2 so that the rope 23c is in a stretched and straightened state, and the mouse 5 is initially positioned by the multiple combination of the side positioner 21, the second pressing piece 22, the mandibular positioner and the mouse mouth positioner 23.
The inner cylinder 30 is connected with a compression bar 35a through a third connecting piece 35, when initial positioning is performed, the compression bar 35a is in compression joint with the rope 23c, when the inner cylinder 30 moves downwards, the compression bar 35a pushes down the middle part of the rope 23c to move downwards, so that the rope 23c in the mouse mouth positioner 23 is driven to tighten, and meanwhile, the plurality of third pressing pieces 23d overcome the tension of the second elastic piece 23g to tighten the mouse mouth, and the mouse mouth cannot shake when stressed.
Preferably, referring to fig. 4, each third pressing piece 23d has a gathered extension 23e thereon, which is more suitable for the mouth shape of a mouse.
Preferably, the invention further comprises a abdominal clamp 20a for fixing the waist of the mouse 5, wherein the upper end of the abdominal clamp 20a is connected with a first screw rod 20 which is vertically arranged, and the upper end of the first screw rod 20 is in threaded connection with the first sliding block 11b, so that the abdominal clamp 20a moves downwards to press the abdomen of the mouse 5 on the base 1 by screwing the first screw rod 20.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A skull positioning device of a mouse hypothalamic area, which comprises a base (1) for fixing a mouse (5), and is characterized by also comprising a mouse head positioning mechanism (2) for positioning and clamping the head of the mouse (5); the mouse head positioning mechanism (2) comprises a side positioner (21) for fixing the cheek face skull at two sides of the mouse (5) and a second pressing sheet (22) for fixing the head top of the mouse (5);
a platform (12) is supported on the base (1) through a bracket (10), and a driving mechanism (3) is arranged on the platform (12) and used for driving a side positioner (21) and the second pressing piece (22) to position and clamp the head of the mouse (5);
the driving mechanism (3) comprises an outer cylinder (31) vertically arranged on the platform (12) and an inner cylinder (30) which is arranged in the outer cylinder (31) in a vertically sliding manner, the inner cylinder (30) is used for accommodating the injector (6), and a third elastic piece (32) is arranged between the outer cylinder (31) and the inner cylinder (30) and used for driving the inner cylinder (30) to move upwards; the lower end of the inner cylinder body (30) is provided with an extension cylinder (30 e), the extension cylinder (30 e) penetrates and extends to the lower part of the outer cylinder body (31), and the second pressing sheet (22) is arranged at the lower end part of the extension cylinder (30 e) so that a needle head (60) of the injector (6) can penetrate into the head part of the mouse (5) through the extension cylinder (30 e);
the side locator (21) comprises two groups of first pressing sheets (21 e) which are oppositely arranged and are used for pressing the cheek face skull on two sides of the mouse (5); the side positioner (21) further comprises two sets of threaded pipes (21 a) which are oppositely arranged, each set of threaded pipes (21 a) is connected with a second screw rod (21 b), each set of threaded pipes (21 a) is further provided with a movable part (21 c) in a sliding manner towards the inside of one end of the head of the mouse (5), the first end of each movable part (21 c) is abutted against the corresponding second screw rod (21 b), the second end of each movable part is connected with the corresponding first pressing piece (21 e) through a set of compressed first elastic parts (21 f) so as to push the corresponding movable parts (21 c) and the corresponding first elastic parts (21 f) through screwing the corresponding second screw rods (21 b), so that the corresponding first pressing pieces (21 e) squeeze the cheek bones on two sides of the mouse (5), and a set of pressing pieces (21 d) which are spaced from the corresponding first pressing pieces (21 e) are further arranged at the second ends of the movable parts (21 c);
two groups of vertical rods (33) are further arranged on the inner cylinder body (30), a wedge-shaped part (33 a) is arranged at the lower end of each group of vertical rods (33), the surface of a threaded pipe (21 a) right below the wedge-shaped part (33 a) is provided with a through hole, inclined planes are respectively arranged on the abutting end surfaces of a second screw rod (21 b) in each group of threaded pipes (21 a) and a movable piece (21 c), so that an upward horn-shaped opening is formed between the second screw rod (21 b) and the movable piece (21 c), and the through hole is positioned above the opening;
when the injector (6) is inserted into the inner cylinder (30) to enable the inner cylinder (30) to move downwards, the second pressing piece (22) presses the top of the head of the mouse (5), and the wedge-shaped parts (33 a) at the lower ends of the vertical rods (33) of each group are driven to be inserted into the openings, so that the movable piece (21 c) is driven to move, and the first pressing piece (21 e) is pressed through the pressing piece (21 d).
2. The mouse hypothalamic area skull positioning device of claim 1, wherein: the travel of the second screw (21 b) in the threaded tube (21 a) is not greater than the maximum width of the opening, so that the opening is always located below the through hole.
3. A mouse hypothalamic area skull positioning device according to any of claims 1 or 2, characterized in that: the lower jaw positioner is used for fixing the lower jaw of the mouse (5), and comprises a second sliding block (4) which is axially and movably arranged on the base (1) along the screw rod (11 a), wherein the second sliding block (4) is connected with the base (1) through a group of third screws (41) so that the second sliding block (4) can be fixed at any position of the self moving stroke;
the second sliding block (4) is also provided with a supporting block (40) for supporting the lower jaw of the mouse (5), and the surface of the second sliding block (4) contacted with the lower jaw of the mouse (5) is an arc-shaped surface matched with the outline of the lower jaw of the mouse (5).
4. A mouse hypothalamic area skull positioning device according to claim 3, characterized in that: the second sliding block (4) is also provided with a marker post (42) for positioning a front marinating point (5 a) of the mouse (5), and the marker post (42) is provided with a graduated scale (420) for displaying the distance between the hypothalamus (5 b) of the mouse (5) and the front marinating point (5 a) in the length direction of the screw rod (11 a).
5. The mouse hypothalamic area skull positioning device according to claim 4, wherein: the mouse mouth fixing device is characterized by further comprising a mouse mouth positioner (23) used for fixing the mouth of the mouse (5), wherein the mouse mouth positioner (23) is used for being sleeved on the mouth of the mouse (5), and the mouse mouth positioner (23) is arranged on the second sliding block (4).
6. The mouse hypothalamic area skull positioning device according to claim 5, wherein: the mouse mouth positioner (23) comprises a circular ring (23 a) arranged on the second sliding block (4), a plurality of cap shells (23 b) are arranged on the outer wall of the circular ring (23 a) in a circumferential array, pistons (23 h) are arranged in the cap shells (23 b), the piston (23 h) is connected in each group of cap shells (23 b) through a stretching-shaped second elastic piece (23 g), the tail end of each piston (23 h) is connected with a third pressing piece (23 d) positioned in the circular ring (23 a) through two groups of guide rods (23 f), and the third pressing pieces (23 d) are arranged around the axis of the circular ring (23 a);
the outer walls of the plurality of third pressing pieces (23 d) are sleeved by a group of ropes (23 c), the ropes (23 c) sleeved on the outer walls of the plurality of third pressing pieces (23 d) are penetrated between two groups of guide rods (23 f), the tail ends of the ropes (23 c) penetrate through the circular rings (23 a) and are sleeved on the bracket (10), the plurality of third pressing pieces (23 d) are sleeved on the mouse mouth, and the second sliding blocks (4) move towards the mouse mouth to enable the ropes (23 c) to be in a stretching shape;
and the inner cylinder body (30) is also provided with a pressing rod (35 a) which is in pressure connection with the rope (23 c), and when the inner cylinder body (30) moves downwards, the pressing rod (35 a) downwards presses to enable the rope (23 c) to tighten the plurality of third pressing sheets (23 d) so as to fasten the mouse mouth.
7. The mouse hypothalamic area skull positioning device of claim 1, wherein: a screw pair (11) is arranged on a support (10) of the base (1), the screw pair (11) comprises a screw rod (11 a) rotatably arranged on the support (10) and a first sliding block (11 b) arranged on the screw rod (11 a), the screw rod (11 a) is horizontally arranged, a guide piece (11 c) is horizontally arranged on the support (10), and the first sliding block (11 b) is slidably arranged on the guide piece (11 c) in a penetrating manner so that the first sliding block (11 b) can horizontally reciprocate along the length direction of the screw rod (11 a);
the guide piece (11 c) is also slidably provided with the platform (12), the platform (12) is in driving connection with the first sliding block (11 b), the first sliding block (11 b) drives the platform (12) to synchronously move, and the driving mechanism (3) and the side positioner (21) are arranged on the platform (12).
8. The mouse hypothalamic area skull positioning device of claim 7, wherein: the abdomen clamp (20 a) for fixing the waist of the mouse (5) is further included, a first screw rod (20) which is vertically arranged is connected to the upper end of the abdomen clamp (20 a), the upper end of the first screw rod (20) is in threaded connection with the first sliding block (11 b), and the abdomen clamp (20 a) is enabled to move downwards through screwing the first screw rod (20) so as to press the abdomen of the mouse (5) on the base (1).
9. The mouse hypothalamic area skull positioning device of claim 8, wherein: the first screw rod (20) vertically penetrates through the platform (12), and the first sliding block (11 b) is in driving connection with the platform (12) through the first screw rod (20).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111123097.1A CN113813076B (en) | 2021-09-24 | 2021-09-24 | Skull positioning device for hypothalamus region of mouse |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111123097.1A CN113813076B (en) | 2021-09-24 | 2021-09-24 | Skull positioning device for hypothalamus region of mouse |
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| CN113813076A CN113813076A (en) | 2021-12-21 |
| CN113813076B true CN113813076B (en) | 2023-11-21 |
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| CN208838226U (en) * | 2018-04-24 | 2019-05-10 | 安徽师范大学 | The adapter fixed for mouse brain solid |
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| CN111388135A (en) * | 2020-03-26 | 2020-07-10 | 河南中医药大学 | Fixing device for functional experiment |
| CN211301543U (en) * | 2019-07-17 | 2020-08-21 | 吴周强 | Rat trigeminal ganglion injection guide plate |
| CN213283540U (en) * | 2020-04-20 | 2021-05-28 | 广西中医药大学 | Laboratory mouse mouth cover fixing device |
| CN113101004A (en) * | 2021-04-22 | 2021-07-13 | 广西医科大学 | Rat mouth gag |
| CN113164036A (en) * | 2018-09-21 | 2021-07-23 | 麦科鲁金克斯有限公司 | Methods, devices and systems for ophthalmic testing and measurement |
| CN213941053U (en) * | 2020-11-25 | 2021-08-13 | 深圳华阳生物技术有限公司 | Mouse head fixing device |
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2021
- 2021-09-24 CN CN202111123097.1A patent/CN113813076B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208838226U (en) * | 2018-04-24 | 2019-05-10 | 安徽师范大学 | The adapter fixed for mouse brain solid |
| CN209285798U (en) * | 2018-06-15 | 2019-08-23 | 千奥星科南京生物科技有限公司 | A kind of stereotaxic instrument |
| CN113164036A (en) * | 2018-09-21 | 2021-07-23 | 麦科鲁金克斯有限公司 | Methods, devices and systems for ophthalmic testing and measurement |
| CN211301543U (en) * | 2019-07-17 | 2020-08-21 | 吴周强 | Rat trigeminal ganglion injection guide plate |
| CN111388135A (en) * | 2020-03-26 | 2020-07-10 | 河南中医药大学 | Fixing device for functional experiment |
| CN213283540U (en) * | 2020-04-20 | 2021-05-28 | 广西中医药大学 | Laboratory mouse mouth cover fixing device |
| CN213941053U (en) * | 2020-11-25 | 2021-08-13 | 深圳华阳生物技术有限公司 | Mouse head fixing device |
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| CN113813076A (en) | 2021-12-21 |
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