CN113802395B - Blue lyocell fabric prepared by dyeing escherichia coli fermentation extract and preparation method thereof - Google Patents
Blue lyocell fabric prepared by dyeing escherichia coli fermentation extract and preparation method thereof Download PDFInfo
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Abstract
The invention relates to blue lyocell fabric prepared by dyeing escherichia coli fermentation extract and a preparation method thereof. The invention adopts indole as substrate for colibacillus, and the indigo pigment is prepared by culturing and purifying; mixing indigo pigment, phenol oxidase solution, cellulase solution and H 2 O 2 Preparing a dyeing solution by using a solution and the like, putting manganese dioxide, the lyocell fabric and the dyeing solution into a dyeing machine for dyeing, inactivating enzyme of the dyed lyocell fabric, and then washing and drying with tap water to obtain the blue lyocell fabric. According to the invention, cellulose and indigo pigment are respectively activated by selecting cellulase and polyphenol oxidase, so that the graft polymerization reaction of cellulose and indigo pigment is realized. The dyeing condition of the invention is mild, and the pollution to the environment is small; the dyed blue lyocell fabric has higher K/S value and higher soaping fastness and dry rubbing fastness grades.
Description
Technical Field
The invention belongs to the technical field of fabric preparation, and particularly relates to blue lyocell fabric prepared by dyeing escherichia coli fermentation extract and a preparation method thereof.
Background
As early as in the ancient times, humans began to dye textile fabrics with natural dyes. In the 19 th century, a large number of synthetic dyes which are cheap, convenient to prepare and wide in color spectrum gradually occupy the market. Heretofore, synthetic dyes have been largely used in various industries such as textile, leather, paper, food, etc., but some of them have been disabled due to carcinogenic, allergenic effects of synthetic precursors or products on the human body. Along with the improvement of the living standard of people, the living concept of health and environmental protection is beginning to be favored. Natural dye is nontoxic, has no carcinogenicity, has good biocompatibility and is degradable, and is currently considered as an environment-friendly chemical capable of replacing synthetic dye, so that enthusiasm of students for developing and researching the natural dye is stimulated.
Natural dyes produced by plants, animals and microorganisms are considered to be several major sources of possible alternatives to synthetic dyes at present. The most widely used in the food and textile industries are plant extracts and microbial ferments. Although some plants (such as ginkgo, yew and mulberry) contain natural pigments, the growth cycle of the plants is long, and cultivation occupies a large amount of land resources, so that the industrial production of the natural pigments is limited to a certain extent. Microorganisms such as bacteria and fungi can stably produce natural pigments by fermentation culture methods, such as: beta-carotene, red organic amines, indigo pigments, and the like. Common chromogenic bacteria include Brevibacterium flavum, staphylococcus aureus, pseudomonas aeruginosa, serratia viscosa, violet bacillus, etc. The microbial fermentation method has high pigment yield, less residue compared with animals and plants, and low space and environment requirements. The generated chromophoric group of the natural dye can be further chemically modified to obtain a wider spectrum. In addition, some anthraquinone microbial dyes are bright in color and have a certain antibacterial effect, which indicates that some anthraquinone microbial dyes have potential application values in functional finishing of fabrics.
Indigo pigment is one of the important sources of natural blue pigment, and can be obtained by fermentation of escherichia coli. The literature research shows that the indigo pigment is widely applied in a plurality of fields, but the application of the indigo pigment to the dyeing of textiles is rarely reported. The indigo pigment has relatively stable chemical property, and is not easy to react with textiles, so that the indigo pigment has certain limitation in textile dyeing application and needs to be broken through further.
Disclosure of Invention
The chemical synthetic dye has strong reactivity, is combined with the lyocell fabric by chemical bonds, and the indigo pigment is relatively stable and has poor reactivity, so that the dye is not easy to combine with the lyocell fabric; the indigo can be used for dyeing the lyocell fabric under the auxiliary action of the printing and dyeing auxiliary agent. The dyeing process of the lyocell fabric by the indigo under the assistance of the printing and dyeing auxiliary agent can cause environmental pollution and a large amount of resource consumption; in addition, the lyocell fabric prepared by the dyeing process has low dyeing fastness, washing resistance and low weather fastness. Aiming at the defects in the prior art, the invention aims to provide blue lyocell fabric prepared by dyeing escherichia coli fermentation extract and a preparation method thereof.
The invention aims to provide a blue lyocell fabric prepared by dyeing escherichia coli fermentation extract, which has higher K/S value and better soaping discoloration resistance and dry rubbing color resistance.
The invention aims to provide blue lyocell fabric prepared by dyeing escherichia coli fermentation extract, which can be prepared by the following preparation method: the colibacillus adopts indole as a substrate, and is cultured and purified to prepare the indigo pigment; preparing phenol oxidase solution and cellulase solution, adding indigo pigment, phenol oxidase solution, cellulase solution and H 2 O 2 Preparing a dyeing liquid by using aqueous solution and the like, putting manganese dioxide, the lyocell fabric and the dyeing liquid into a dyeing machine for dyeing, inactivating enzyme of the dyed lyocell fabric, washing with tap water and drying to obtain the blue lyocell fabric.
The invention also aims to provide a preparation method of blue lyocell fabric prepared by dyeing escherichia coli fermentation extract, which comprises the following steps:
(1) Purification of E.coli fermentation extract: the purification method comprises the following steps:
s1: preparation of seed culture medium: glucose 1.0-1.2 g/L, potassium dihydrogen phosphate 0.5-0.7 g/L, and magnesium sulfate 0.2-0.3 g/L;
s2: preparation of fermentation medium: 0.1 to 0.2g/L of indole, 1.0 to 1.2g/L of agar, 1.0 to 1.2g/L of monopotassium phosphate, 0.2 to 0.3g/L of magnesium sulfate and 0.2 to 0.3g/L of calcium chloride;
s3: seed culture: inoculating colibacillus into a seed culture medium according to 1-1.2% of inoculum size under aseptic condition, and culturing for 48-60 h under the conditions of 28-30 ℃ and 150-200 r/min of rotation speed;
s4: fermentation culture: under the aseptic condition, inoculating the cultured seed culture solution into a sterilized fermentation culture medium with an inoculum size of 10-12%; fermenting and culturing for 100-120 h under the conditions of pH=6.0-7.0, temperature 28-30 ℃ and rotating speed 220-300 r/min;
s5: purification of E.coli fermentation extract: filtering the culture solution with gauze, vacuum drying wet thalli at 45-55 ℃, and weighing dry weight after constant weight; crushing the dried and constant-weight thalli to 60-100 meshes by using a crusher, adding absolute ethyl alcohol for extraction until the thalli are colorless, filtering an extract liquid, collecting the extract liquid, and distilling the extract liquid under reduced pressure to obtain an escherichia coli fermentation extract, wherein the extract is indigo pigment powder.
(2) Preparing an enzyme solution: preparing polyphenol oxidase solution: dissolving polyphenol oxidase in phosphate buffer solution with pH of 6.3-6.7 to prepare polyphenol oxidase solution with concentration of 20-30 mg/mL; preparing a cellulase solution: dissolving cellulase in an acetic acid-sodium acetate buffer solution with the pH value of 4.6-5.0 to prepare 20-30 mg/mL cellulase solution;
preferably, the preparation method of the phosphate buffer solution comprises the following steps: taking 0.6-0.7 g of monopotassium phosphate, adding 15-16 mL of 0.08-0.12 mol/L sodium hydroxide solution, and diluting with water to 80-120 mL to obtain the aqueous solution;
preferably, the preparation method of the acetic acid-sodium acetate buffer solution comprises the following steps: dissolving 5.0-6.0 g of sodium acetate in 40-60 mL of water, regulating the pH value to 4.6-5.0 by glacial acetic acid, and diluting to 80-120 mL by adding water.
(3) Preparing a staining solution: indigo pigment powder, polyphenol oxidase solution, cellulose solution, 8-12wt% H 2 O 2 Adding distilled water into the aqueous solution, stirring and mixing to obtain a dyeing solution, and regulating the pH value of the dyeing solution to 6-7 by using 30wt% NaOH alkali liquor;
preferably, the indigo pigment powder (g), polyphenol oxidase solution (mL), cellulase solution (mL), 8-12wt% H 2 O 2 The dosage ratio of the aqueous solution (mL) to distilled water (mL) is as follows: 1:0.1-0.3:0.1-0.3:0.5-0.7:50-70.
(4) Dyeing: mnO is added to 2 Putting the lyocell fabric and the dyeing liquid into the dyeing machineDyeing by a color machine, wherein the bath ratio is 1:20-30, the dyeing time is 30-40 minutes, the dyeing temperature is 30-50 ℃, and the lyocell fabric is taken out after the dyeing is finished;
preferably, the MnO 2 The amount (g) of the dye solution is the ratio of the amount (mL) of the dye solution to the amount (mL): 0.02 to 0.06 percent.
(5) Post-treatment: inactivating enzyme in boiling water for 60-80 min, washing the inactivated lyocell fabric with tap water for 2-4 times, and drying to obtain blue lyocell fabric.
Analysis of the dyeing mechanism of the invention:
during the dyeing process, H 2 O 2 At MnO 2 Under the condition of acting as a catalyst, oxygen is continuously and slowly generated, and the reaction equation is shown as follows.
Polyphenol Oxidase (PPO) is an enzyme specific for anthraquinone compounds, and the fermented extract of escherichia coli is an indigo pigment under the condition that indole is used as a culture substrate. The PPO will catalyze the oxidation of indigo to reactive free radical in the presence of oxygen as follows:
cellulases are a multicomponent enzyme system comprising a plurality of hydrolase members and are generally classified into the following 3 classes: (a) Endoglucanases, which hydrolyze beta-1, 4 glycosidic bonds in the amorphous region inside cellulose molecules, cutting long-chain cellulose molecules into short chains, and generating a large amount of small-molecule cellulose; (b) Exoglucanases which act on the ends of the polysaccharide chains to hydrolyze the beta-1, 4 glycosidic bonds, each time cleaving one cellobiose molecule from the non-reducing end, so are also known as cellobiohydrolases; (c) Beta-glucosidase, which hydrolyzes cellobiose to glucose molecules. It can be seen that the cellulose-related chemical bonds (e.g., beta-1, 4 glycosidic bonds) which are the main component of lyocell are slowly broken under the action of cellulase.
In the process of decomposing cellulose by cellulose, when chemical bonds of cellulose are broken, the indigo pigment with active free radicals is encountered, and at the moment, the indigo pigment with active free radicals attacks the chemical bond groups of the cellulose which are just broken, and the two chemical bonds react, so that the indigo pigment is grafted on the cellulose.
The invention has the following remarkable advantages:
(1) Aiming at the characteristic of low reactivity of the lyocell fiber, the invention selects the cellulase to activate the reactivity of the lyocell fiber, and in the process, the invention properly selects the low-dose cellulase, thus realizing the reactivity of activating the lyocell fiber without damaging the molecular structure of cellulose.
(2) The colibacillus adopts indole as a substrate, and is cultured and purified to prepare the indigo pigment; the indigo pigment is blue, the molecular structure of the blue pigment is an anthraquinone compound, and the chemical property is stable; aiming at the characteristic of low reactivity of the blue pigment, the invention selects polyphenol oxidase to activate the reactivity of the blue pigment, so that the blue pigment generates anthraquinone compounds with reactive free radicals.
(3) The inventors of the present application have unexpectedly found that graft polymerization of cellulose and blue pigment is achieved by selecting cellulase and polyphenol oxidase to activate cellulose and blue pigment, respectively, to produce cellulose and blue pigment having reactivity.
(4) The blue lyocell fabric dyed by the escherichia coli fermentation extract prepared by the invention has a K/S value slightly larger than that of the blue lyocell fabric purchased in the market, and the level of the soaping-resistant color-changing fastness and the dry friction-resistant color fastness is slightly higher than that of the blue lyocell fabric purchased in the market.
(5) Aiming at the defects of large environmental pollution and large resource consumption caused by chemical dye dyeing, the invention adopts cellulase and polyphenol oxidase for dyeing, has mild dyeing conditions and small environmental pollution.
Detailed Description
The examples and comparative examples described below illustrate the invention in detail.
The main raw material sources are as follows: polyphenol oxidase (870U/mg) was purchased from Worthington Biotechnology development Co., ltd, cellulase (40U/mg) was purchased from Shanghai Source leaf Biotechnology Co., ltd, and E.coli was purchased from Shanghai Biochemical reagent Co.
Example 1
The preparation method of the blue lyocell fabric prepared by dyeing the escherichia coli fermentation extract comprises the following steps of:
(1) Purification of E.coli fermentation extract: the purification method comprises the following steps:
s1: preparation of seed culture medium: glucose 1.1g/L, potassium dihydrogen phosphate 0.6g/L, and magnesium sulfate 0.25g/L;
s2: preparation of fermentation medium: indole 0.15g/L, agar 1.1g/L, potassium dihydrogen phosphate 1.1g/L, magnesium sulfate 0.25g/L, and calcium chloride 0.25g/L;
s3: seed culture: inoculating Escherichia coli into a seed culture medium according to an inoculum size of 1.1% under a sterile condition, and culturing for 54h at a temperature of 29 ℃ and a rotating speed of 180 r/min;
s4: fermentation culture: under the aseptic condition, inoculating the cultured seed culture solution into a sterilized fermentation culture medium in an inoculum size of 11%; fermenting and culturing for 110h under the conditions of pH=6.8, temperature 29 ℃ and rotating speed 260 r/min;
s5: purification of E.coli fermentation extract: filtering the culture solution with gauze, vacuum drying wet thallus at 50deg.C, weighing dry weight after constant weight; pulverizing dried thallus with constant weight to 80 mesh with pulverizer, extracting with anhydrous ethanol until thallus is colorless, filtering the extractive solution, collecting extractive solution, and distilling the extractive solution under reduced pressure to obtain fermented extract of Escherichia coli which is indigo pigment powder.
(2) Preparing an enzyme solution: preparing polyphenol oxidase solution: dissolving polyphenol oxidase in phosphate buffer solution with pH of 6.5 to prepare 25mg/mL polyphenol oxidase solution; preparing a cellulase solution: dissolving cellulase in an acetic acid-sodium acetate buffer solution with the pH value of 4.8 to prepare a cellulase solution with the concentration of 25 mg/mL; the preparation method of the phosphate buffer solution comprises the following steps: taking 0.68g of monopotassium phosphate, adding 15.2mL of 0.1mol/L sodium hydroxide solution, and diluting with water to 100mL to obtain the potassium dihydrogen phosphate; the preparation method of the acetic acid-sodium acetate buffer solution comprises the following steps: dissolving 5.4g of sodium acetate in 50mL of water, adjusting the pH value to 4.8 with glacial acetic acid, and diluting to 100mL with water.
(3) Preparing a staining solution: 1g of indigo pigment powder, 0.2mL of polyphenol oxidase solution, 0.2mL of cellulase solution, 0.6mL of 10wt% H 2 O 2 60mL of distilled water is added into the aqueous solution, and the mixture is stirred and mixed to prepare a staining solution, and the pH value of the staining solution is regulated and controlled to 6.5 by 30wt% NaOH alkali liquor.
(4) Dyeing: 0.04g MnO 2 Putting the lyocell fabric and 100mL of dyeing liquid into a dyeing machine for dyeing, wherein the bath ratio is 1:25, the dyeing time is 35 minutes, the dyeing temperature is 40 ℃, and taking out the lyocell fabric after dyeing is finished.
(5) Post-treatment: inactivating enzyme in boiling water for 70min, washing the inactivated lyocell fabric with tap water for 3 times, and drying to obtain blue lyocell fabric.
Example 2
The preparation method of the blue lyocell fabric prepared by dyeing the escherichia coli fermentation extract comprises the following steps of:
(1) Purification of E.coli fermentation extract: the purification method comprises the following steps:
s1: preparation of seed culture medium: glucose 1.0g/L, potassium dihydrogen phosphate 0.5g/L, and magnesium sulfate 0.2g/L;
s2: preparation of fermentation medium: indole 0.1g/L, agar 1.0g/L, potassium dihydrogen phosphate 1.0g/L, magnesium sulfate 0.2g/L, and calcium chloride 0.2g/L;
s3: seed culture: inoculating Escherichia coli into a seed culture medium according to 1% of inoculation amount under aseptic conditions, and culturing for 48h at the temperature of 28 ℃ and the rotating speed of 150 r/min;
s4: fermentation culture: under the aseptic condition, inoculating the cultured seed culture solution into a sterilized fermentation culture medium with an inoculum size of 10 percent; fermenting and culturing for 100h under the conditions of pH=6.0, temperature 28 ℃ and rotating speed 220 r/min;
s5: purification of E.coli fermentation extract: filtering the culture solution with gauze, vacuum drying wet thallus at 45deg.C, weighing dry weight after constant weight; pulverizing dried thallus with constant weight to 60 mesh with pulverizer, extracting with anhydrous ethanol until thallus is colorless, filtering the extractive solution, collecting extractive solution, and distilling the extractive solution under reduced pressure to obtain fermented extract of Escherichia coli which is indigo pigment powder.
(2) Preparing an enzyme solution: preparing polyphenol oxidase solution: dissolving polyphenol oxidase in phosphate buffer solution with pH of 6.5 to prepare 25mg/mL polyphenol oxidase solution; preparing a cellulase solution: dissolving cellulase in an acetic acid-sodium acetate buffer solution with the pH value of 4.8 to prepare a cellulase solution with the concentration of 25 mg/mL; the preparation method of the phosphate buffer solution comprises the following steps: taking 0.68g of monopotassium phosphate, adding 15.2mL of 0.1mol/L sodium hydroxide solution, and diluting with water to 100mL to obtain the potassium dihydrogen phosphate; the preparation method of the acetic acid-sodium acetate buffer solution comprises the following steps: dissolving 5.4g of sodium acetate in 50mL of water, adjusting the pH value to 4.8 with glacial acetic acid, and diluting to 100mL with water.
(3) Preparing a staining solution: 1g of indigo pigment powder, 0.1mL of polyphenol oxidase solution, 0.1mL of cellulase solution, 0.5mL of 10wt% H 2 O 2 50mL of distilled water is added into the aqueous solution, and the mixture is stirred and mixed to prepare a staining solution, and the pH value of the staining solution is regulated to 6.5 by 30wt% NaOH alkali liquor.
(4) Dyeing: will be 0.02gMnO 2 Putting the lyocell fabric and 100mL of dyeing liquid into a dyeing machine for dyeing, wherein the bath ratio is 1:20, the dyeing time is 30 minutes, the dyeing temperature is 30 ℃, and taking out the lyocell fabric after dyeing is finished.
(5) Post-treatment: inactivating enzyme in boiling water for 60min, washing the inactivated lyocell fabric with tap water for 2 times, and drying to obtain blue lyocell fabric.
Example 3
The preparation method of the blue lyocell fabric prepared by dyeing the escherichia coli fermentation extract comprises the following steps of:
(1) Purification of E.coli fermentation extract: the purification method comprises the following steps:
s1: preparation of seed culture medium: glucose 1.2g/L, potassium dihydrogen phosphate 0.7g/L, magnesium sulfate 0.3g/L;
s2: preparation of fermentation medium: indole 0.2g/L, agar 1.2g/L, potassium dihydrogen phosphate 1.2g/L, magnesium sulfate 0.3g/L, and calcium chloride 0.3g/L;
s3: seed culture: inoculating Escherichia coli into a seed culture medium according to 1.2% of inoculation amount under aseptic conditions, and culturing for 60h at the temperature of 30 ℃ and the rotating speed of 200 r/min;
s4: fermentation culture: under the aseptic condition, inoculating the cultured seed culture solution into a sterilized fermentation culture medium in an inoculum size of 12 percent; fermenting and culturing for 120h under the conditions of pH=7.0, temperature 30 ℃ and rotating speed 300 r/min;
s5: purification of E.coli fermentation extract: filtering the culture solution with gauze, vacuum drying wet thallus at 55deg.C, weighing dry weight after constant weight; pulverizing dried thallus with constant weight to 100 mesh with pulverizer, extracting with anhydrous ethanol until thallus is colorless, filtering the extractive solution, collecting extractive solution, and distilling the extractive solution under reduced pressure to obtain fermented extract of Escherichia coli which is indigo pigment powder.
(2) Preparing an enzyme solution: preparing polyphenol oxidase solution: dissolving polyphenol oxidase in phosphate buffer solution with pH of 6.5 to prepare 25mg/mL polyphenol oxidase solution; preparing a cellulase solution: dissolving cellulase in an acetic acid-sodium acetate buffer solution with the pH value of 4.8 to prepare a cellulase solution with the concentration of 25 mg/mL; the preparation method of the phosphate buffer solution comprises the following steps: taking 0.68g of monopotassium phosphate, adding 15.2mL of 0.1mol/L sodium hydroxide solution, and diluting with water to 100mL to obtain the potassium dihydrogen phosphate; the preparation method of the acetic acid-sodium acetate buffer solution comprises the following steps: dissolving 5.4g of sodium acetate in 50mL of water, adjusting the pH value to 4.8 with glacial acetic acid, and diluting to 100mL with water.
(3) Preparing a staining solution: 1g of indigo pigment powder, 0.3mL of polyphenol oxidase solution, 0.3mL of cellulase solution, 0.7mL of 10wt% H 2 O 2 70mL of distilled water is added into the aqueous solution, and the mixture is stirred and mixed to prepare a staining solution, and the pH value of the staining solution is regulated to 6.5 by 30wt% NaOH alkali liquor.
(4) Dyeing: 0.06g MnO 2 Putting the lyocell fabric and 100mL of dyeing liquid into a dyeing machine for dyeing, wherein the bath ratio is 1:30, the dyeing time is 40 minutes, the dyeing temperature is 50 ℃, and taking out the lyocell fabric after dyeing is finished.
(5) Post-treatment: inactivating enzyme in boiling water for 80min, washing the inactivated lyocell fabric with tap water for 4 times, and drying to obtain blue lyocell fabric.
Comparative example 1
In this comparative example, using example 1 as a comparison, only the polyphenol oxidase solution was added in step (2), and no cellulase solution was added, and the other production methods were carried out as in example 1.
Comparative example 2
In this comparative example, using example 1 as a comparison, only the cellulase solution was added in step (2), and no polyphenol oxidase solution was added, and the other production methods were carried out as in example 1.
Evaluation of performance:
performance evaluation was performed on the blue lyocell fabrics obtained in examples 1 to 3 and comparative examples 1 to 2 and the blue lyocell fabrics purchased in the market, which were purchased from Jiangsu Hua textile Co., ltd. The K/S value is measured by a computer color measuring and matching instrument; the soaping-resistant color-changing fastness value test refers to GB/T3921-2008 "soaping-resistant color fastness for textile color fastness test"; dry rub fastness value test reference GB/T3920-2008 "rubbing fastness to textile color fastness test", specific data are shown in table 1.
TABLE 1
| Sample of | K/S value | Fastness to soaping | Dry rub fastness/grade |
| Example 1 | 11.65 | 5 | 5 |
| Example 2 | 11.26 | 4-5 | 5 |
| Example 3 | 11.43 | 5 | 5 |
| Comparative example 1 | 5.58 | 3 | 3 |
| Comparative example 2 | 5.29 | 3 | 3 |
| Market purchasing | 10.58 | 4-5 | 4-5 |
As can be seen from Table 1, the K/S values, the soaping discoloration fastness and the dry rub fastness of examples 1 to 3 are all better than the K/S values, the soaping discoloration fastness and the dry rub fastness of comparative examples 1 to 2; in addition, the K/S value of the blue lyocell fabric prepared by the method is slightly higher than that of the blue lyocell fabric purchased in the market.
Claims (5)
1. The preparation method of the blue lyocell fabric prepared by dyeing the escherichia coli fermentation extract is characterized by comprising the following steps of:
(1) Preparing a staining solution: indigo pigment powder, polyphenol oxidase solution, cellulose solution, 8-12wt% H 2 O 2 Adding distilled water into the aqueous solution, stirring and mixing to obtain a dyeing solution, and regulating the pH value of the dyeing solution to 6-7 by using 30wt% NaOH alkali liquor;
(2) Dyeing: mnO is added to 2 Putting the lyocell fabric and the dyeing liquid into a dyeing machine for dyeing, wherein the bath ratio is 1:20-30, the dyeing time is 30-40 minutes, the dyeing temperature is 30-50 ℃, and taking out the lyocell fabric after dyeing is finished;
(3) Post-treatment: inactivating enzyme in boiling water, washing the inactivated lyocell fabric with tap water for 2-4 times, and drying to obtain blue lyocell fabric;
the preparation method of the indigo pigment powder in the step (1) comprises the following steps:
s1: preparation of seed culture medium: glucose 1.0-1.2 g/L, potassium dihydrogen phosphate 0.5-0.7 g/L, and magnesium sulfate 0.2-0.3 g/L;
s2: preparation of fermentation medium: 0.1 to 0.2g/L of indole, 1.0 to 1.2g/L of agar, 1.0 to 1.2g/L of monopotassium phosphate, 0.2 to 0.3g/L of magnesium sulfate and 0.2 to 0.3g/L of calcium chloride;
s3: seed culture: inoculating colibacillus into a seed culture medium according to 1-1.2% of inoculum size under aseptic condition, and culturing for 48-60 h under the conditions of 28-30 ℃ and 150-200 r/min of rotation speed;
s4: fermentation culture: under the aseptic condition, inoculating the cultured seed culture solution into a sterilized fermentation culture medium with an inoculum size of 10-12%; fermenting and culturing for 100-120 h under the conditions of pH=6.0-7.0, temperature 28-30 ℃ and rotating speed 220-300 r/min;
s5: purification of E.coli fermentation extract: filtering the culture solution with gauze, vacuum drying wet thalli at 45-55 ℃, and weighing dry weight after constant weight; crushing the dried and constant-weight thalli to 60-100 meshes by using a crusher, adding absolute ethyl alcohol for extraction until the thalli are colorless, filtering an extract, collecting the extract, and distilling the extract under reduced pressure to obtain an escherichia coli fermentation extract, wherein the extract is indigo pigment powder;
the preparation method of the polyphenol oxidase solution in the step (1) comprises the following steps: dissolving polyphenol oxidase in phosphate buffer solution with pH of 6.3-6.7 to prepare polyphenol oxidase solution with concentration of 20-30 mg/mL;
the preparation method of the cellulase solution in the step (1) comprises the following steps: dissolving cellulase in an acetic acid-sodium acetate buffer solution with the pH value of 4.6-5.0 to prepare 20-30 mg/mL cellulase solution;
the indigo pigment powder, polyphenol oxidase solution, cellulose solution and 8-12wt% H in the step (1) 2 O 2 The dosage ratio of the solution to distilled water is as follows: 1g to (0.1-0.3) mL to (0.5-0.7) mL to (50-70) mL;
the MnO in the step (2) 2 The ratio of the dosage of the dye to the dosage of the dye solution is as follows: 0.02% -0.06% (g/mL).
2. The method for preparing blue lyocell fabric dyed by escherichia coli fermentation extract according to claim 1, wherein the preparation method of the phosphate buffer solution is as follows: taking 0.6-0.7 g of monopotassium phosphate, adding 15-16 mL of 0.08-0.12 mol/L sodium hydroxide solution, and diluting with water to 80-120 mL.
3. The method for preparing blue lyocell fabric dyed by escherichia coli fermentation extract according to claim 1, wherein the preparation method of the acetic acid-sodium acetate buffer solution is as follows: dissolving 5.0-6.0 g of sodium acetate in 40-60 mL of water, regulating the pH value to 4.6-5.0 by glacial acetic acid, and diluting to 80-120 mL by adding water.
4. The method for preparing blue lyocell fabric dyed by escherichia coli fermentation extract according to claim 1, wherein the enzyme deactivation time in the step (3) is 60-80 min.
5. The blue lyocell fabric dyed by the escherichia coli fermentation extract is characterized by being prepared by a preparation method of the blue lyocell fabric dyed by the escherichia coli fermentation extract according to any one of claims 1 to 4.
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