CN113801822B - Bacillus cereus YZS-C10 and application thereof - Google Patents
Bacillus cereus YZS-C10 and application thereof Download PDFInfo
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- CN113801822B CN113801822B CN202111151301.0A CN202111151301A CN113801822B CN 113801822 B CN113801822 B CN 113801822B CN 202111151301 A CN202111151301 A CN 202111151301A CN 113801822 B CN113801822 B CN 113801822B
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- 241000193755 Bacillus cereus Species 0.000 title claims abstract description 37
- 239000004698 Polyethylene Substances 0.000 claims abstract description 48
- 229920000573 polyethylene Polymers 0.000 claims abstract description 48
- -1 polyethylene Polymers 0.000 claims abstract description 41
- 229920003023 plastic Polymers 0.000 claims abstract description 9
- 239000004033 plastic Substances 0.000 claims abstract description 9
- 238000006065 biodegradation reaction Methods 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims abstract description 5
- 238000009629 microbiological culture Methods 0.000 claims abstract description 4
- 230000015556 catabolic process Effects 0.000 claims description 14
- 238000006731 degradation reaction Methods 0.000 claims description 14
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 230000008859 change Effects 0.000 description 10
- 238000005259 measurement Methods 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 125000000524 functional group Chemical group 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000011707 mineral Substances 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 240000002044 Rhizophora apiculata Species 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical group CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
- 238000009642 citrate test Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000009654 indole test Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 241001249119 Bacillus vallismortis Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000010292 electrical insulation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- CSJDCSCTVDEHRN-UHFFFAOYSA-N methane;molecular oxygen Chemical compound C.O=O CSJDCSCTVDEHRN-UHFFFAOYSA-N 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000013502 plastic waste Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000004416 thermosoftening plastic Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29B—PREPARATION OR PRETREATMENT OF THE MATERIAL TO BE SHAPED; MAKING GRANULES OR PREFORMS; RECOVERY OF PLASTICS OR OTHER CONSTITUENTS OF WASTE MATERIAL CONTAINING PLASTICS
- B29B17/00—Recovery of plastics or other constituents of waste material containing plastics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/62—Plastics recycling; Rubber recycling
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- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Mechanical Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides bacillus cereus YZS-C10 and application thereof, and belongs to the technical field of microorganisms. The classification of the bacillus cereus YZS-C10 is named as bacillus cereus @Bacillus vallismortis) YZS-C10 was deposited in China general microbiological culture Collection center, with the accession number: CGMCC No.22857, the preservation address is Hospital No.1 and No. 3 of North Chen West road in the Chaoyang area of Beijing city. The bacillus cereus YZS-C10 has good effect on the biodegradation of polyethylene plastics, provides new resources and solutions for the biodegradation of polyethylene wastes in the environment, and has great application prospect.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus YZS-C10 and application thereof in biodegradation of polyethylene plastics.
Background
Plastics are important organic synthesesPolymerThe material is very widely applied. However, plastics are difficult to degrade naturally in the environment, and the "white pollution" caused by waste plastics is also more and more serious. Polyethylene (PE) isEthyleneOne obtained by polymerizationThermoplastic resin. Polyethylene is odorless, nontoxic, wax-like in hand feeling, has excellent low temperature resistance,chemical stabilityAnd the product is good in resistance to most of acid and alkali. Is insoluble in general at normal temperatureSolvent(s)，Water absorptionThe size of the product is small and the product is easy to be manufactured,electrical insulation propertyExcellent, is easy to accumulate in natural environment, and causes secondary pollution. Therefore, the technology of disposing of plastic such as waste polyethylene is urgent, and the current research on biodegradation of plastic waste such as polyethylene is less and effectiveLittle microbial resource and low treatment efficiency.
Mangrove grows in the tropical, subtropical offshore intertidal zone and is immersed in periodic seawater for a long period of time. The water-saving type flood control system has the functions of flood prevention, wave prevention, storm prevention, dike protection and the like, and has unique social and economic values in the aspects of maintaining the carbon-oxygen balance of the atmosphere, purifying the atmosphere and the water environment and the like. The special habitat of mangrove determines its abundant microbial resources, and reserves genetic resources different from other environmental microbial sources.
Disclosure of Invention
The invention aims to provide a bacillus cereus strain and application thereof.
In order to achieve the above purpose, the invention adopts the following technical scheme:
bacillus cereus YZS-C10, which is classified and named as Bacillus cereus @Bacillus vallismortis) YZS-C10 was deposited in China general microbiological culture Collection center, with the accession number: CGMCC No.22857, the preservation address is Hospital No.1 and No. 3 of North Chen West road in the Chaoyang area of Beijing city.
The colony characteristics and the thallus morphology of the dead bacillus cereus YZS-C10 are as follows:
the bacterial colony formed by culturing YZS-C10 strain on NA flat plate culture medium for 24h is light yellow, matt, round, free of fluidity, gram positive, spore and capsule-free; the colony diameter is 3-4mm.
Physiological and biochemical characteristics of the bacillus cereus YZS-C10:
the YZS-C10 strain has negative contact enzyme reaction, V.P measurement is positive, MR measurement is negative, glucose acidogenesis test is positive, glucose acidogenesis test is negative, citrate test is negative, nitrate reduction is positive, starch hydrolysis is positive, indole test is negative, malonic acid measurement is negative, and H is produced 2 S test positive.
The result of comparing the 16S rDNA sequence of the dead bacillus cereus YZS-C10 with the sequences in the GenBank database shows that the YZS-C10 is compared with the sequences in the GenBank databaseBacillus vallismortisOn one branch, its 16S rDNA sequence andBacillus vallismortis（KP994551.1) The similarity of (2) reaches 100%. Combining colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, and primarily identifying the bacillus cereus as the bacillus cereus @Bacillus vallismortis）。
The invention has the advantages that:
the bacillus cereus YZS-C10 has good effect on the biodegradation of polyethylene plastics, provides new resources and solutions for the biodegradation of polyethylene wastes in the environment, and has great application prospect.
Drawings
Figure 1 topographical features of PE films in an optical microscope after 30 days of degradation by Bacillus cereus YZS-C10. a control; b inoculating bacillus cereus YZS-C10.
Figure 2 is a graph showing the morphological characteristics of PE films in a scanning electron microscope after 30 days of degradation of Bacillus cereus YZS-C10. a control; b inoculating bacillus cereus YZS-C10.
FIG. 3 shows the change of water contact angle of PE film before and after degradation of Bacillus cereus YZS-C10.
FIG. 4 shows the change of functional groups on the surface of PE film before degradation of Bacillus cereus YZS-C10.
FIG. 5 shows the change of functional groups on the surface of PE film after degradation of Bacillus cereus YZS-C10.
Detailed Description
The invention is further illustrated by the following examples.
Bacillus cereus YZS-C10, which is classified and named as Bacillus cereus @Bacillus vallismortis) YZS-C10 was deposited in China general microbiological culture Collection center, with the accession number: CGMCC No.22857, the preservation address is Hospital No.1 and No. 3 of North Chen West road in the Chaoyang area of Beijing city.
EXAMPLE 1 isolation screening and identification of Bacillus cereus
(1) And (3) separating and screening:
319 isolates were isolated from Fujian mangrove plants by gradient dilution. Purifying the separated endophytic bacteria by a three-zone streak method, judging whether the bacterial strain is purified by microscopic examination, numbering the purified bacteria, picking single bacterial colonies, and transferring the single bacterial colonies to an NA slant culture medium for storage. The surface of a plate of a carbon source-free mineral salt solid medium is coated with 0.02 g/plate (the diameter of the plate is 9 cm) of sterilized polyethylene powder, the strain obtained above is subjected to streak culture on the surface, and single colonies which grow rapidly are screened to be cultured and stored on an NA plate medium. And 7 strains with better polyethylene degradation effect are initially screened out through growth measurement of a carbon source-free mineral salt culture medium with polyethylene as a unique carbon source, and endophytic bacteria with high-efficiency degradation polyethylene plastic are finally screened out, and marked as YZS-C10.
The culture medium for screening is a mineral salt culture medium without carbon source, and comprises the following components: k (K) 2 HPO 4 ·3H 2 O 0.7 g，KH 2 PO 4 0.7 g，NH 4 NO 3 1.0 g，NaCl 0.5 g，MgSO 4 ·7H 2 O 0.5 g，FeSO 4 ·7H 2 O 0.002 g，ZnSO 4 ·7H 2 O 0.002 g，MnSO 4 ·H 2 O0.001 g; the above components were dissolved in 1L deionized water and the pH was adjusted to 7.0 with 1 mol/L NaOH. 1.5wt% agar was added to the solid medium.
Through the separation and screening work, the bacillus cereus YZS-C10 which is the endophyte of polyethylene degradation and grows rapidly is obtained through multiple separation and purification.
(2) Colony characteristics and colony morphology:
after the YZS-C10 strain is cultured on an NA plate for 24 hours, a colony is light yellow, matt, round, free of fluidity, gram-positive, spore-bearing and capsule-free; the colony diameter is 3-4mm.
(3) Physiological and biochemical characteristics:
the YZS-C10 strain has negative contact enzyme reaction, V.P measurement is positive, MR measurement is negative, glucose acidogenesis test is positive, glucose acidogenesis test is negative, citrate test is negative, nitrate reduction is positive, starch hydrolysis is positive, indole test is negative, malonic acid measurement is negative, and H is produced 2 S test positive.
(4) 16S rDNA sequence analysis
The 16S rDNA gene sequence is shown in a nucleotide sequence table SEQ ID NO. 1. The result of comparing the detected 16S rDNA sequence with the sequences in GenBank database shows that YZS-C10 is compared withBacillus vallismortisOn one branch, its 16S rDNA sequence andBacillus vallismortis（KP994551.1) The similarity of (2) reaches 100%. Combining colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, and primarily identifying to be bacillus cereus @Bacillus vallismortis）。
EXAMPLE 2 degradation Effect of Bacillus cereus on polyethylene film
In order to evaluate the application of the bacillus cereus YZS-C10 in polyethylene degradation, a common polyethylene film is selected for degradation effect measurement.
Bacillus cereusBacillus vallismortis) YZS-C10 is activated on NA slant culture medium, and one loop is selected and inoculated into NA culture medium (beef extract 3g, peptone 10g, naCl 5g, agar 18g, water 1000ml, pH 7.0-7.2) for culturing to logarithmic phase. Cutting polyethylene film into 1×1 cm pieces 2 After weighing, 2 h was sterilized with 75vol% ethanol, and then the surface ethanol was volatilized off with a sterile air stream in an ultra clean bench. 100 mL carbon-free mineral salt liquid culture medium is added into a conical flask, 3 pieces of surface sterilized polyethylene film are added, and the YZS-C10 logarithmic growth phase culture solution is inoculated with an inoculum size of 1% (v/v). The control group was a sterile treatment, and 3 replicates were set for each treatment. After placing the conical flask in a constant temperature shaking incubator at 25 ℃ and 180 r/min for culturing for 30 d, samples are taken to measure the mass loss, the surface morphology change, the functional group change and the hydrophobicity change of the polyethylene film.
And (3) measuring the mass change of the polyethylene film: the polyethylene film sheet 2 h was washed with 2wt% SDS solution by shaking, sonicated for 15 min, the biofilm on the surface was removed, the film sheet was washed 3 times with sterile water, and the washed polyethylene film sheet was dried in a desiccator for 48 h and then weighed. Mass loss rate (%) = (initial mass of polyethylene-mass after degradation)/initial mass×100% of polyethylene.
And (3) measuring the microscopic morphology change of the surface of the polyethylene film: excess medium on the surface was rinsed with sterile water, fixed with 2.5wt% glutaraldehyde for 2 h, and dehydrated sequentially with 30vol%, 50vol%, 70vol%, 90vol%, 100vol% ethanol for 15 min each, and replaced with t-butanol for 2 times each for 30 min. Observing the surface morphology change of the treated sample by adopting an optical microscope; microscopic morphological features were observed under a scanning electron microscope (Hitachi SU 3800) after fixed metal spraying.
The change in surface hydrophobicity of the polyethylene film was measured using a contact angle measuring instrument.
Surface functional group measurement of polyethylene film: naturally drying the cleaned polyethylene film, measuring with Fourier infrared spectrometer, and scanning wavelength range of 600-4000 cm -1 Resolution 4 cm -1 The number of scans was 32.
As can be seen from Table 1, when the polyethylene film was cultured to 30 d in the above-described culture manner, the mass of the polyethylene film inoculated with YZS-C10 treatment was reduced by 0.30mg on average, and the mass loss ratio was 5.85%. The control treatment was unchanged, indicating degradation of the polyethylene film by YZS-C10.
TABLE 1 influence of YZS-C10 on the mass loss of polyethylene films
As can be seen from FIGS. 1 and 2, after 30 d culture, the inoculated Bacillus cereus was observed under an optical microscope and a scanning electron microscopeBacillus vallismortis) Compared with the control group, the YZS-C10 treated polyethylene film has rough surface, disintegrates at the edge, and has obvious microorganism erosion holes on the surface.
As can be seen from FIG. 3, the results of the contact angle measurement show that the bacillus cereus is inoculatedBacillus vallismortis) The water contact angle of the polyethylene film treated by YZS-C10 is 73.57 +/-2.17 degrees, the water contact angle of the polyethylene film treated by the control treatment is 91.40+/-0.79 degrees, which is lower than that of the polyethylene film treated by the control treatment by 17.83 degrees, and the water contact angle is reduced by 19.51 percent, which shows that the water contact angle of the polyethylene film treated by the sterilization treatment is obviously reduced, and the polyethylene film is more easily adhered and corroded by microorganisms.
As can be seen from FIGS. 4 and 5, the inoculation of Bacillus cereusBacillus vallismortis) The infrared spectrum of the polyethylene film treated by YZS-C10 is 1652 cm -1 A new characteristic peak, i.e. a carbonyl peak, appears. Carbonyl [ -C=O-bond]The appearance of (2) is a fundamental sign of PE biodegradation, indicating that polyethylene is biodegradable.
The foregoing description is only of the preferred embodiments of the invention, and all changes and modifications that come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
SEQUENCE LISTING
<110> Minjiang college
<120> Bacillus vallismortis YZS-C10 strain and application thereof
<130> 1
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1424
<212> DNA
<213> SEQ ID NO.1
<400> 1
ttcggcggct ggctcctaaa ggttacctca ccgacttcgg gtgttacaaa ctctcgtggt 60
gtgacgggcg gtgtgtacaa ggcccgggaa cgtattcacc gcggcatgct gatccgcgat 120
tactagcgat tccagcttca cgcagtcgag ttgcagactg cgatccgaac tgagaacaga 180
tttgtgggat tggcttaacc tcgcggtttc gctgcccttt gttctgtcca ttgtagcacg 240
tgtgtagccc aggtcataag gggcatgatg atttgacgtc atccccacct tcctccggtt 300
tgtcaccggc agtcacctta gagtgcccaa ctgaatgctg gcaactaaga tcaagggttg 360
cgctcgttgc gggacttaac ccaacatctc acgacacgag ctgacgacaa ccatgcacca 420
cctgtcactc tgcccccgaa ggggacgtcc tatctctagg attgtcagag gatgtcaaga 480
cctggtaagg ttcttcgcgt tgcttcgaat taaaccacat gctccaccgc ttgtgcgggc 540
ccccgtcaat tcctttgagt ttcagtcttg cgaccgtact ccccaggcgg agtgcttaat 600
gcgttagctg cagcactaag gggcggaaac cccctaacac ttagcactca tcgtttacgg 660
cgtggactac cagggtatct aatcctgttc gctccccacg ctttcgctcc tcagcgtcag 720
ttacagacca gagagtcgcc ttcgccactg gtgttcctcc acatctctac gcatttcacc 780
gctacacgtg gaattccact ctcctcttct gcactcaagt tccccagttt ccaatgaccc 840
tccccggttg agccgggggc tttcacatca gacttaagaa accgcctgcg agccctttac 900
gcccaataat tccggacaac gcttgccacc tacgtattac cgcggctgct ggcacgtagt 960
tagccgtggc tttctggtta ggtaccgtca aggtgccgcc ctatttgaac ggcacttgtt 1020
cttccctaac aacagagctt tacgatccga aaaccttcat cactcacgcg gcgttgctcc 1080
gtcagacttt cgtccattgc ggaagattcc ctactgctgc ctcccgtagg agtctgggcc 1140
gtgtctcagt cccagtgtgg ccgatcaccc tctcaggtcg gctacgcatc gtcgccttgg 1200
tgagccgtta cctcaccaac tagctaatgc gccgcgggtc catctgtaag tggtagccga 1260
agccaccttt tatgtctgaa ccatgcggtt caaacaacca tccggtatta gccccggttt 1320
cccggagtta tcccagtctt acaggcaggt tacccacgtg ttactcaccc gtccgccgct 1380
aacatcaggg agcaagctcc catctgtccg ctcgacttgc atgt 1424
Claims (2)
1. The bacillus cereus YZS-C10 strain is characterized in that: the classification of the dead bacillus cereus YZS-C10 is named as dead bacillus cereus @Bacillus vallismortis) YZS-C10 is preserved in China general microbiological culture Collection center (CGMCC) at 7 and 9 days of 2021, with a preservation number of CGMCC No.22857 and a preservation address of North Chenxi Lu No.1 and 3 in the Chaoyang area of Beijing; the 16S rDNA gene sequence of the dead bacillus cereus YZS-C10 is shown in SEQ ID NO. 1.
2. The application of the dead bacillus cereus YZS-C10 in the biodegradation of polyethylene plastics, which is characterized in that: the temperature of the degradation was 25 ℃.
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| CN111690553A (en) * | 2020-05-18 | 2020-09-22 | 西北农林科技大学 | Bacterium for degrading plastic mulching film and application thereof |
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