CN113800622A

CN113800622A - Method and device for removing photoinhibition of photosynthetic microorganisms and application of method and device

Info

Publication number: CN113800622A
Application number: CN202111182203.3A
Authority: CN
Inventors: 孙健; 姚欣媛; 白晓燕
Original assignee: Guangdong University of Technology
Current assignee: Guangdong University of Technology
Priority date: 2021-10-11
Filing date: 2021-10-11
Publication date: 2021-12-17
Anticipated expiration: 2041-10-11
Also published as: CN113800622B

Abstract

The application belongs to the technical field of bioelectrochemistry, and particularly relates to a method and a device for removing photoinhibition of photosynthetic microorganisms and application thereof. The present application provides a method of removing photoinhibition of photosynthetic microorganisms; wherein, the method for releasing the photoinhibition of the photosynthetic microorganisms comprises the following steps: the photosynthetic microorganism is illuminated to provide hydrogen and carbon sources for the photosynthetic microorganism and attach the photosynthetic microorganism on the working electrode, and part of generated photosynthetic electrons are transferred from the photosynthetic microorganism to the working electrode, so that the photosynthesis of the photosynthetic microorganism is promoted to synthesize organic matters such as protein and the like and the synthesized organic matters are metabolized in vivo, and the photoinhibition of the photosynthetic microorganism is relieved.

Description

Method and device for removing photoinhibition of photosynthetic microorganisms and application of method and device

Technical Field

The application belongs to the technical field of bioelectrochemistry, and particularly relates to a method and a device for removing photoinhibition of photosynthetic microorganisms and application thereof.

Background

Photosynthetic microorganisms are microorganisms living by using light as the only or main energy source, and can synthesize organic matters in vivo by utilizing various inorganic matters and organic matters in the nature under the illumination condition, so the treatment of the photosynthetic microorganisms is a common method for removing inorganic and organic pollutants in wastewater.

However, the self-protection reaction of the photosynthetic system of the photosynthetic microorganism itself, i.e., the photoinhibition of the photosynthetic microorganism, is specifically embodied in the phenomenon that when the illumination intensity exceeds the intensity that the photosynthetic system of the photosynthetic microorganism itself can utilize, the photosynthetic function of the photosynthetic microorganism is reduced, so that the efficiency of removing inorganic and organic pollutants in wastewater and synthesizing organic matters such as protein in vivo by the existing photosynthetic microorganism is not high.

Disclosure of Invention

In view of the above, the present application provides a method and an apparatus for removing photoinhibition of photosynthetic microorganisms and applications thereof, which promote the growth and metabolism of photosynthetic microorganisms by applying electrical stimulation to the photosynthetic microorganisms, and can extract more photosynthetic electrons, thereby removing photoinhibition of photosynthetic microorganisms, and solving the problem that in the prior art, when the intensity of light exceeds the intensity that can be utilized by the photosynthetic system of the photosynthetic microorganisms themselves, the photosynthetic function of photosynthetic microorganisms is reduced, and photoinhibition occurs.

In a first aspect, the present application provides a method for removing photoinhibition of photosynthetic microorganisms comprising the steps of:

step one, applying light to the photosynthetic microorganisms;

step two, providing a hydrogen body and carbon source for the photosynthetic microorganism;

thirdly, the photosynthetic microorganisms are attached to an electrode;

step four, applying electrode potential to the electrode;

the electrode is a working electrode in a three-electrode electrochemical system.

It should be noted that, photosynthetic microorganisms are attached to the working electrode in the three-electrode electrochemical system, and since the three-electrode electrochemical system forms a closed loop and simultaneously applies electrode potential, the growth and metabolism of the photosynthetic microorganisms are stimulated, more photosynthetic electrons can be extracted and transferred to the working electrode, so that extracellular diversion is realized, photosynthetic metabolism of the photosynthetic microorganisms is enhanced, and the purpose of removing photoinhibition of the photosynthetic microorganisms is achieved.

Preferably, the applying an electrode potential comprises: an electrode potential of-0.3V, 0V, +0.3V was applied.

It should be noted that although the electrical stimulation accelerates the growth and metabolism of the photosynthetic microorganisms and releases the photoinhibition, if the applied electrode potential is too large, a large amount of photosynthetic microorganisms will die, and therefore, controlling the magnitude of the applied electrode potential is advantageous to avoid the death of a large amount of photosynthetic microorganisms.

Preferably, the hydrogen and carbon source includes: one, two or more of ammonia nitrogen, phosphate and sulfadiazine.

It should be noted that ammonia nitrogen, phosphate and sulfadiazine are common inorganic and organic pollutants in domestic and industrial wastewater, and photosynthetic microorganisms growing in the domestic and industrial wastewater can adapt to the domestic and industrial wastewater containing ammonia nitrogen, phosphate and sulfadiazine due to natural acclimation, and grow, metabolize and synthesize protein and other organic matters by using the ammonia nitrogen, phosphate and sulfadiazine for photosynthesis.

A second aspect of the present application provides a device for implementing a method for removing the photoinhibition of photosynthetic microorganisms, comprising:

photosynthetic microorganism photosynthetic reactor, electrochemical workstation;

the working electrode of the electrochemical workstation is arranged in the photosynthetic microorganism photosynthetic reactor.

It should be noted that the photosynthetic microorganisms in the photosynthetic microorganism photosynthetic reactor can utilize hydrogen and carbon sources to perform photosynthesis, synthesize organic matters in vivo and metabolize the organic matters to generate photosynthetic electrons, and the photosynthetic microorganisms are loaded on the working electrode of the electrochemical workstation, so that the working electrode can transfer the photosynthetic electrons generated in the photosynthesis of the photosynthetic microorganisms and the metabolic process of the organic matters, the photosynthesis of the photosynthetic microorganisms is promoted, and the photoinhibition of the photosynthetic microorganisms is relieved.

Preferably, the photosynthetic microorganism photosynthetic reactor comprises a light source, a container;

the container may contain a gas, a liquid or a solid containing a hydrogen and carbon source and a culture solution of the photosynthetic microorganism, and the working electrode loaded with the photosynthetic microorganism is placed in the gas, the liquid or the solid containing the hydrogen and carbon source, and the photosynthetic microorganism is photosynthetic to synthesize an organic substance and the organic substance decomposed and synthesized in the body is metabolized under irradiation of the light source simulated sunlight.

Preferably, the working electrode is a graphite plate.

It should be noted that, graphite has excellent stability and conductivity, and when used as a working electrode of an electrochemical workstation, can avoid interference, transfer photosynthetic electrons, and well reflect photosynthesis and growth metabolism of photosynthetic microorganisms on the working electrode.

Preferably, the light source is one, two or more of a torch, a candle, an incandescent lamp, a sodium lamp, a mercury lamp, a fluorescent lamp, an LED and an OLED.

Preferably, the light source is an OLED.

The relative color temperature (CCT) of the incandescent lamp is about 2700K, the relative color temperature (CCT) of the cold fluorescent lamp is about 4000-5000K and is far lower than 2500-8000K of sunlight, and the relative color temperature (CCT) of the OLED is close to the sunlight, so that the OLED has the performance of simulating the sunlight, and the photosynthetic microorganisms can be better promoted to utilize hydrogen and carbon sources to carry out photosynthesis.

Preferably, the graphite plates have dimensions of 30 × 20 × 5 mm.

Preferably, the graphite plate is a treated graphite plate.

Preferably, the processing method comprises: and sequentially putting the graphite plate into acetone and deionized water for ultrasonic treatment for 10min and 20min respectively, and then putting the graphite plate into an oven for drying for later use. And (4) ultrasonically treating the electrode for 20min by using deionized water, and then putting the electrode into an oven for drying.

Preferably, the distance between the light source and the working electrode is 5 cm.

Preferably, the container is a glass container.

It should be noted that the glass container has good light transmittance, so that the light of the light source can be irradiated on the surface of the photosynthetic microorganism as much as possible, and the growth and metabolism of the photosynthetic microorganism are accelerated.

In a third aspect, the present application provides a method and apparatus for removing the photoinhibition of photosynthetic microorganisms for use in the field of wastewater treatment.

Nitrogen and phosphorus contained in the wastewater promote the growth of algae and aquatic plants, thereby deteriorating the water quality and reducing dissolved oxygen. The organic pollutant antibiotic in the waste water coexists with the pollutant containing nitrogen and phosphorus, the antibiotic can affect the environment at extremely low concentration, and has the characteristics of durability, degradability and accumulation in the environment, the existing efficiency of treating the waste water by utilizing the photosynthetic microorganism is not high due to the photoinhibition phenomenon of the photosynthetic microorganism, and the growth and metabolism and photosynthesis of the photosynthetic microorganism can be promoted by removing the photoinhibition of the photosynthetic microorganism, so that the aim of improving the efficiency of treating the waste water by the photosynthetic microorganism is fulfilled.

In summary, the present application provides a method and apparatus for removing the photoinhibition of photosynthetic microorganisms and uses thereof; wherein, the method for releasing the photoinhibition of the photosynthetic microorganisms comprises the following steps: the photosynthetic microorganism is illuminated, hydrogen and carbon sources are provided for the photosynthetic microorganism, the photosynthetic microorganism is attached to the working electrode, when the photosynthetic microorganism utilizes the hydrogen and carbon sources to carry out photosynthesis under the illumination condition, organic matters are synthesized in vivo and the organic matters are produced along with the production of photosynthetic electrons in the in vivo metabolic process of the photosynthetic microorganism, and when part of produced photosynthetic electrons are transferred from the photosynthetic microorganism to the working electrode, the photosynthesis of the photosynthetic microorganism is promoted to synthesize the organic matters and the synthesized organic matters are metabolized in vivo, so that the photoinhibition of the photosynthetic microorganism is relieved; therefore, the present application provides a method and apparatus for removing the photo-inhibition of photosynthetic microorganisms and the application thereof, which can solve the technical problem of the prior art that when the illumination intensity exceeds the intensity that the photosynthetic system of the photosynthetic microorganisms can utilize, the photosynthetic function of the photosynthetic microorganisms is reduced.

Description of the drawings:

in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.

FIG. 1 shows the ammonia nitrogen removal efficiency of the present application at 3000Lux illumination intensity;

FIG. 2 shows the ammonia nitrogen removal efficiency of the present application at 8000Lux illumination intensity;

FIG. 3 shows the ammonia nitrogen removal efficiency of the present application at 23000Lux illumination intensity;

FIG. 4 is a graph of phosphate removal at 3000Lux illumination intensity for the present application;

FIG. 5 is a graph of phosphate removal at 8000Lux illumination according to the present application;

FIG. 6 is a graph of phosphate removal at 23000Lux illumination in accordance with the present application;

FIG. 7 is a graph of the effect of antibiotic degradation in the present application at 3000Lux illumination;

FIG. 8 is a graph of the effect of antibiotic degradation in the present application at 8000Lux illumination;

FIG. 9 is a graph of the effect of antibiotic degradation in 23000Lux light intensity for the present application;

FIG. 10 is a histogram of protein content of photosynthetic microorganisms under illumination intensity of 3000Lux, 8000Lux, 23000Lux in the present application.

The specific implementation mode is as follows:

the application provides a method and a device for removing the photoinhibition of photosynthetic microorganisms and application thereof, which can solve the technical problem of the phenomenon that the photosynthetic function of the photosynthetic microorganisms is reduced when the illumination intensity exceeds the intensity which can be utilized by the photosynthetic systems of the photosynthetic microorganisms in the prior art.

The technical solutions in the embodiments of the present application will be described clearly and completely below, and it should be understood that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

The reagents or raw materials used in the following examples are commercially available or self-made.

Example 1

The present embodiment provides a first method for removing the photoinhibition of photosynthetic microorganisms, comprising the steps of:

step one, a graphite plate is used as a working electrode of an electrochemical workstation, a titanium wire is used as a counter electrode, and a saturated calomel electrode is used as a reference electrode to construct a three-electrode electrochemical system;

placing the graphite plate working electrode loaded with the photosynthetic microorganisms, the titanium wire counter electrode, the saturated calomel electrode and the photosynthetic microorganism culture solution into a glass container to form a closed loop;

controlling the OLED lamp to irradiate the glass container with light intensities of 3000lux, 8000lux and 23000 lux;

and step four, applying 0V electrode potential to a working electrode of the three-electrode electrochemical system.

Wherein the photosynthetic microorganisms are collected in the factory wastewater and are in a logarithmic growth phase, and the concentration of ammonia nitrogen in the photosynthetic microorganism culture solution is 125 mg/L.

It should be noted that, the working electrode, the counter electrode and the reference electrode in the three-electrode electrochemical system form a closed loop, and under the condition that 0V electrode potential is applied to the reference electrode, the photosynthetic microorganisms are attached to the working electrode to electrically stimulate the photosynthetic microorganisms, so that the growth and metabolism are accelerated, and more photosynthetic electrons generated in the growth and metabolism process are guided to the outside of cells through the working electrode, so that the photosynthetic metabolism of the photosynthetic microorganisms is promoted, and the purpose of removing the photoinhibition of the photosynthetic microorganisms is achieved.

Example 2

This example provides a second method of removing photoinhibition of photosynthetic microorganisms, which differs from example 1 in that it further comprises a fifth step of applying a potential of-0.3V through an electrochemical workstation.

Example 3

This example provides a third method for the removal of photoinhibition of photosynthetic microorganisms, which differs from example 1 in that it further comprises a fifth step of applying a potential of +0.3V through an electrochemical workstation.

Example 4

This embodiment provides a fourth method for removing photoinhibition of photosynthetic microorganisms, comprising the steps of:

Wherein the photosynthetic microorganisms are collected in the factory wastewater and are in a logarithmic growth phase, and the concentration of phosphate in a photosynthetic microorganism culture solution is 15 mg/L.

Example 5

This example provides a fifth method for removing the photoinhibition of photosynthetic microorganisms, which comprises the step five of applying a potential of-0.3V through an electrochemical workstation.

Example 6

This example provides a sixth method for removing the photoinhibition of photosynthetic microorganisms, which comprises the step five of applying a potential of +0.3V through the electrochemical workstation, different from the step 4.

Example 7

The present embodiment provides a seventh method for removing the photoinhibition of photosynthetic microorganisms, comprising the steps of:

Wherein the photosynthetic microorganism is collected in the factory wastewater and is in a logarithmic growth phase, and the concentration of sulfadiazine in the photosynthetic microorganism culture solution is 1 mg/L.

Example 8

This example provides an eighth method for removing the photoinhibition of photosynthetic microorganisms, which comprises the step five of applying a potential of-0.3V through an electrochemical workstation.

Example 9

This example provides a ninth method for removing the photoinhibition of photosynthetic microorganisms, which comprises the step five of applying a potential of +0.3V through an electrochemical workstation.

Example 10

The present embodiment provides a tenth method for removing the photoinhibition of photosynthetic microorganisms, comprising the steps of:

placing factory wastewater loaded with a photosynthetic microorganism graphite plate working electrode, a titanium wire counter electrode, a saturated calomel electrode and a photosynthetic microorganism culture solution in a glass container to form a closed loop;

Wherein the photosynthetic microorganism is collected in the factory wastewater and is in a logarithmic growth phase, the concentration of ammonia nitrogen in a photosynthetic microorganism culture solution is 125mg/L, the concentration of phosphate is 15mg/L, the concentration of sulfadiazine is 1mg/L, and the photosynthetic microorganism culture solution is prepared by a conventional formula method in the field.

Example 11

This example provides an eleventh method for removing photoinhibition of photosynthetic microorganisms comprising the steps of, in addition to step five, applying a potential of-0.3V through an electrochemical workstation.

Example 12

The present embodiment provides a twelfth method for removing the photoinhibition of photosynthetic microorganisms, which comprises the step five of applying a potential of +0.3V through the electrochemical workstation, different from the step 10.

Comparative example 1:

placing photosynthetic microorganisms loaded in a glass container containing a photosynthetic microorganism culture solution;

and step two, controlling the OLED lamp to irradiate the glass container with light intensities of 3000lux, 8000lux and 23000 lux.

The photosynthetic microorganism is collected in factory wastewater and is in a logarithmic growth phase, the concentration of ammonia nitrogen in a photosynthetic microorganism culture solution is 125mg/L, the concentration of phosphate is 15mg/L, the concentration of sulfadiazine is 1mg/L, and a preparation method of the photosynthetic microorganism culture solution is the prior art and is not described herein.

Example 13

This example is an example of testing the efficiency of removing ammonia nitrogen from the culture broth of photosynthetic microorganisms in the method of removing photoinhibition of photosynthetic microorganisms described in examples 1-3.

The detection step comprises: step one, when an electrochemical workstation detects three repeatable currents, timing is started, and parts of photosynthetic microorganism culture solution containing ammonia nitrogen are taken out at 0 th, 6 th, 12 th, 19 th, 24 th, 36 th, 48 th, 72 th, 96 th and 120 th hours and are respectively marked as samples N1-N10;

step two, filtering the samples N1-N10 by using a water system filter membrane with the aperture of 0.22 mu m, and then placing the filtered samples in a refrigerator at 4 ℃ for storage to be tested;

and step three, measuring the ammonia nitrogen concentration in the filtered samples N1-N10 by adopting a nano reagent spectrophotometry, wherein the results are shown in the attached figures 1-3 of the specification.

Example 14

This example is an example of testing the efficiency of removing phosphate from the culture of photosynthetic microorganisms in the methods for removing the photoinhibition of photosynthetic microorganisms described in examples 4-6.

The detection step comprises: step one, when an electrochemical workstation detects three repeatable currents, timing is started, and partial photosynthetic microorganism culture solution containing phosphate is taken out at 0 th, 6 th, 12 th, 19 th, 24 th, 36 th, 48 th, 72 th, 96 th and 120 th hours and is respectively marked as samples P1-P10;

filtering the samples P1-P10 by using a water system filter membrane with the aperture of 0.22 mu m, and then placing the filtered samples in a refrigerator at 4 ℃ for storage to be tested;

and step three, measuring the phosphate concentration in the filtered samples P1-P10 by adopting an ammonium molybdate spectrophotometry, wherein the results are shown in the attached figures 4-6 of the specification.

Example 15

This example is an example of testing the removal efficiency of sulfadiazine from the culture of photosynthetic microorganisms in the methods of removing photoinhibition of photosynthetic microorganisms described in examples 7-9.

The detection step comprises: step one, when an electrochemical workstation detects three repeatable currents, timing is started, and parts of a photosynthetic microorganism culture solution containing sulfadiazine are taken out at 0 th, 3 rd, 6 th, 12 th, 24 th, 36 th, 48 th, 72 th, 96 th and 120 th hours and are respectively marked as samples S1-S10;

step two, filtering the samples S1-S10 by a water system filter membrane with the aperture of 0.22 mu m, and then placing the filtered samples in a refrigerator at 4 ℃ for storage to be tested;

and step three, adopting high performance liquid chromatography to measure sulfadiazine concentration in the filtered samples S1-S10, and referring to the attached figures 7-9 of the specification.

Example 16

This example is an example of measuring the protein content in photosynthetic microorganisms in the method of releasing photoinhibition of photosynthetic microorganisms described in examples 10-12.

The detection step comprises: step one, applying an electrode potential of 0V to a working electrode under the illumination intensity of 3000lux, scraping the photosynthetic biofilm attached to the working electrode from the working electrode by using a sterile brush when three repeatable currents are detected by an electrochemical workstation, recording the illumination time h of photosynthetic microorganisms, and centrifuging at 4000rpm for 5min so as to collect the photosynthetic microorganisms as much as possible. Protein was extracted using the total protein extraction kit. Add 500. mu.l TPEBuffer-I per 100. mu.l cell pellet. The tubes were vortexed for 1-2 minutes and then placed on ice. For each 100. mu.l of TPEBuffer-I used, 60. mu.l of TPE Buffer-II was added. Gently mix by tapping the tube with a finger. The tube was vortexed for 30 seconds to achieve complete mixing, if necessary. The test tube was placed in a boiling hot water bath for 30 seconds.

Remove the tube and vortex for 30 seconds. This heating and vortexing process was repeated until a clear solution was seen. The tube was incubated in a boiling water bath for a further 10 minutes and after removal the tube was centrifuged at 15000Xg for 5 minutes at 4 ℃ to remove all cell debris. The supernatant, i.e., the protein-containing solution, was transferred to a clean tube. The supernatant can be stored at the temperature of between 20 ℃ below zero and 70 ℃ and is recorded as a sample CH 1;

step two to step nine, under 3000lux illumination intensity, under-0.3V electrode potential, 3000lux illumination intensity, +0.3V electrode potential and 8000lux illumination intensity, under 0V electrode potential, 8000lux illumination intensity, -0.3V electrode potential, 8000lux illumination intensity, +0.3V electrode potential and 23000lux illumination intensity, under 0V electrode potential, 23000lux illumination intensity, -0.3V electrode potential, 23000lux illumination intensity, and a sample collected under +0.3V electrode potential is marked as CH 2-CH 9;

step ten, determining the protein content in the filtered samples CH 1-CH 9 by using a BCA protein concentration determination kit, and referring to the attached figure 10 of the specification.

Example 17

This example is an example of measuring the protein content in the photosynthetic microorganism described in comparative example 1.

Step one, illuminating for h hours under the illumination intensity of 3000lux, and then placing the photosynthetic microorganisms in the glass container in a centrifuge for 5min at 4000rpm by using a sterile brush, thereby collecting the photosynthetic microorganisms as much as possible. Protein was extracted using the total protein extraction kit. Add 500. mu.l TPE Buffer-I per 100. mu.l cell pellet. The tubes were vortexed for 1-2 minutes and then placed on ice. For each 100. mu.l TPE Buffer-I used, 60. mu.l TPE Buffer-II was added. Gently mix by tapping the tube with a finger. The tube was vortexed for 30 seconds to achieve complete mixing, if necessary. The test tube was placed in a boiling hot water bath for 30 seconds. Remove the tube and vortex for 30 seconds. This heating and vortexing process was repeated until a clear solution was seen. The tube was incubated in a boiling water bath for a further 10 minutes and after removal the tube was centrifuged at 15000Xg for 5 minutes at 4 ℃ to remove all cell debris. The supernatant, i.e., the protein-containing solution, was transferred to a clean tube. The supernatant can be stored at the temperature of between 20 ℃ below zero and 70 ℃ and is recorded as a sample CH 10;

step two to step three, respectively illuminating for h hours under 8000lux illumination intensity and 23000lux illumination intensity, and recording collected samples as CH 11-CH 12;

step four, the BCA protein concentration determination kit is adopted to determine the protein content in the filtered samples CH 10-CH 12, and the results are shown in the attached figure 10 of the specification.

As can be understood from fig. 10, when the electrode potential is not applied to stimulate the photosynthetic microorganisms, the protein content in the photosynthetic microorganisms is substantially the same under strong light, medium light and weak light, which indicates that the photosynthetic microorganisms are photo-inhibited when the electrode potential is not applied, and when the electrode potential of-0.3V, 0V, +0.3V is applied, the protein content in the photosynthetic microorganisms is significantly increased, which indicates that the application of the electrode potential can promote the growth and metabolism of the photosynthetic microorganisms, extract more photosynthetic electrons and release the photo-inhibition of the photosynthetic microorganisms.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A method of removing photoinhibition of a photosynthetic microorganism comprising the steps of:

step one, applying light to the photosynthetic microorganisms;

thirdly, the photosynthetic microorganisms are attached to an electrode;

step four, applying electrode potential to the electrode;

2. A method for the removal of photo-inhibition of photosynthetic microorganisms according to claim 1 wherein the applying an electrode potential comprises: an electrode potential of-0.3V, 0V, +0.3V was applied.

3. A method for relieving photo-suppression of photosynthetic microorganisms according to claim 1 wherein the hydrogen and carbon source comprises: one, two or three of ammonia nitrogen, phosphate and sulfadiazine.

4. An apparatus for implementing a method for removing the photoinhibition of photosynthetic microorganisms comprising:

5. An apparatus for performing a method of relieving photo-suppression of photosynthetic microorganisms according to claim 4 wherein the photosynthetic microorganism photosynthetic reactor includes a light source, a container.

6. A device for performing a method for the disinhibition of photosynthetic microorganisms according to claim 4, characterized in that the working electrode is a graphite plate.

7. A device for performing a method for the removal of photo-inhibition of photosynthetic microorganisms according to claim 4 characterised in that the light source is one, two or more of a torch, a candle, an incandescent lamp, a sodium lamp, a mercury lamp, a fluorescent lamp, an LED, an OLED.

8. An apparatus for performing a method of photoinhibition of photosynthetic microorganisms according to claim 7 wherein said light source is an OLED.

9. A device for performing a method for the disinhibition of photosynthetic microorganisms according to claim 5, characterized in that the container is a glass container.

10. Use of the method according to claims 1-3 and the device according to claims 4-9 in the field of wastewater treatment.