CN113797219A - Xpr1抑制剂在制备抑制甲状腺癌细胞迁移和/或增殖的产品的应用 - Google Patents
Xpr1抑制剂在制备抑制甲状腺癌细胞迁移和/或增殖的产品的应用 Download PDFInfo
- Publication number
- CN113797219A CN113797219A CN202111068922.2A CN202111068922A CN113797219A CN 113797219 A CN113797219 A CN 113797219A CN 202111068922 A CN202111068922 A CN 202111068922A CN 113797219 A CN113797219 A CN 113797219A
- Authority
- CN
- China
- Prior art keywords
- xpr1
- thyroid cancer
- cells
- cancer cells
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000024770 Thyroid neoplasm Diseases 0.000 title claims abstract description 88
- 201000002510 thyroid cancer Diseases 0.000 title claims abstract description 86
- 108010004696 Xenotropic and Polytropic Retrovirus Receptor Proteins 0.000 title claims abstract description 73
- 102100036974 Xenotropic and polytropic retrovirus receptor 1 Human genes 0.000 title claims abstract description 73
- 238000013508 migration Methods 0.000 title claims abstract description 38
- 230000005012 migration Effects 0.000 title claims abstract description 33
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 32
- 239000003112 inhibitor Substances 0.000 title claims abstract description 30
- 230000035755 proliferation Effects 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 230000014509 gene expression Effects 0.000 claims abstract description 51
- 101100481912 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) tpc-1 gene Proteins 0.000 claims description 10
- 239000008194 pharmaceutical composition Substances 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 6
- 230000008685 targeting Effects 0.000 claims description 5
- 230000002452 interceptive effect Effects 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 abstract description 30
- 230000006907 apoptotic process Effects 0.000 abstract description 18
- 230000009545 invasion Effects 0.000 abstract description 12
- 101150085333 xpr1 gene Proteins 0.000 abstract description 10
- 206010028980 Neoplasm Diseases 0.000 abstract description 8
- 108010082117 matrigel Proteins 0.000 abstract description 8
- 210000001685 thyroid gland Anatomy 0.000 abstract description 5
- 230000002829 reductive effect Effects 0.000 abstract description 4
- 238000010609 cell counting kit-8 assay Methods 0.000 abstract description 3
- 238000000684 flow cytometry Methods 0.000 abstract description 3
- 238000001262 western blot Methods 0.000 abstract description 3
- 230000001332 colony forming effect Effects 0.000 abstract description 2
- 108010087230 Sincalide Proteins 0.000 abstract 1
- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 138
- 108090000623 proteins and genes Proteins 0.000 description 44
- 239000000047 product Substances 0.000 description 30
- 102000004169 proteins and genes Human genes 0.000 description 26
- 239000000243 solution Substances 0.000 description 23
- 239000007788 liquid Substances 0.000 description 21
- 239000012528 membrane Substances 0.000 description 21
- 108020004414 DNA Proteins 0.000 description 14
- 239000001963 growth medium Substances 0.000 description 14
- 239000006228 supernatant Substances 0.000 description 13
- 238000001890 transfection Methods 0.000 description 13
- 108020004459 Small interfering RNA Proteins 0.000 description 10
- 239000004055 small Interfering RNA Substances 0.000 description 10
- 238000005406 washing Methods 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 238000003197 gene knockdown Methods 0.000 description 8
- QZBRCSFGVAKGJU-UVLRMEHCSA-N (e)-2-cyano-3-[4-[(e)-2-[4-(n-phenylanilino)phenyl]ethenyl]phenyl]prop-2-enoic acid Chemical compound C1=CC(/C=C(C(=O)O)\C#N)=CC=C1\C=C\C1=CC=C(N(C=2C=CC=CC=2)C=2C=CC=CC=2)C=C1 QZBRCSFGVAKGJU-UVLRMEHCSA-N 0.000 description 7
- 108010058546 Cyclin D1 Proteins 0.000 description 7
- 102100024165 G1/S-specific cyclin-D1 Human genes 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 230000003247 decreasing effect Effects 0.000 description 7
- 238000001962 electrophoresis Methods 0.000 description 7
- 206010027476 Metastases Diseases 0.000 description 6
- 239000002033 PVDF binder Substances 0.000 description 6
- 230000009401 metastasis Effects 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 102100027995 Collagenase 3 Human genes 0.000 description 5
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 5
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 5
- 238000011529 RT qPCR Methods 0.000 description 5
- 239000006180 TBST buffer Substances 0.000 description 5
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 5
- 102000055102 bcl-2-Associated X Human genes 0.000 description 5
- 238000010009 beating Methods 0.000 description 5
- 239000000090 biomarker Substances 0.000 description 5
- 230000012292 cell migration Effects 0.000 description 5
- 230000004663 cell proliferation Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 5
- 238000010839 reverse transcription Methods 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 108091006027 G proteins Proteins 0.000 description 4
- 102000030782 GTP binding Human genes 0.000 description 4
- 108091000058 GTP-Binding Proteins 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 102000002274 Matrix Metalloproteinases Human genes 0.000 description 4
- 108010000684 Matrix Metalloproteinases Proteins 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 102100026547 Platelet-derived growth factor receptor beta Human genes 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 108091070501 miRNA Proteins 0.000 description 4
- 239000002679 microRNA Substances 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- 238000007747 plating Methods 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101000971171 Homo sapiens Apoptosis regulator Bcl-2 Proteins 0.000 description 3
- 101000577887 Homo sapiens Collagenase 3 Proteins 0.000 description 3
- 101000990902 Homo sapiens Matrix metalloproteinase-9 Proteins 0.000 description 3
- 102100030412 Matrix metalloproteinase-9 Human genes 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108010019160 Pancreatin Proteins 0.000 description 3
- 238000002123 RNA extraction Methods 0.000 description 3
- 102100035100 Transcription factor p65 Human genes 0.000 description 3
- 238000007664 blowing Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000012154 double-distilled water Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 239000006166 lysate Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 229940055695 pancreatin Drugs 0.000 description 3
- 230000002062 proliferating effect Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 108010063104 Apoptosis Regulatory Proteins Proteins 0.000 description 2
- 102000010565 Apoptosis Regulatory Proteins Human genes 0.000 description 2
- -1 BCPAP Chemical compound 0.000 description 2
- 108010001857 Cell Surface Receptors Proteins 0.000 description 2
- 102000000844 Cell Surface Receptors Human genes 0.000 description 2
- 206010066296 Cerebral calcification Diseases 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 101100297529 Drosophila melanogaster pho gene Proteins 0.000 description 2
- 208000001976 Endocrine Gland Neoplasms Diseases 0.000 description 2
- 208000026350 Inborn Genetic disease Diseases 0.000 description 2
- 108010076503 Matrix Metalloproteinase 13 Proteins 0.000 description 2
- 102000001776 Matrix metalloproteinase-9 Human genes 0.000 description 2
- 108010015302 Matrix metalloproteinase-9 Proteins 0.000 description 2
- 208000024556 Mendelian disease Diseases 0.000 description 2
- 241000714177 Murine leukemia virus Species 0.000 description 2
- 101150023810 PHO1 gene Proteins 0.000 description 2
- 108010051742 Platelet-Derived Growth Factor beta Receptor Proteins 0.000 description 2
- 101710164680 Platelet-derived growth factor receptor beta Proteins 0.000 description 2
- 102000052575 Proto-Oncogene Human genes 0.000 description 2
- 108700020978 Proto-Oncogene Proteins 0.000 description 2
- 101100271429 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ATP6 gene Proteins 0.000 description 2
- 101100190361 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PHO90 gene Proteins 0.000 description 2
- 101100115751 Trypanosoma brucei brucei dnaaf11 gene Proteins 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000003021 clonogenic effect Effects 0.000 description 2
- 230000004186 co-expression Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000005757 colony formation Effects 0.000 description 2
- 238000010293 colony formation assay Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 239000003405 delayed action preparation Substances 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 201000011523 endocrine gland cancer Diseases 0.000 description 2
- 238000010201 enrichment analysis Methods 0.000 description 2
- 230000010856 establishment of protein localization Effects 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000036210 malignancy Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000006187 pill Substances 0.000 description 2
- 238000003762 quantitative reverse transcription PCR Methods 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 230000001177 retroviral effect Effects 0.000 description 2
- WDZCUPBHRAEYDL-GZAUEHORSA-N rifapentine Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C(O)=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N(CC1)CCN1C1CCCC1 WDZCUPBHRAEYDL-GZAUEHORSA-N 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- 102000004121 Annexin A5 Human genes 0.000 description 1
- 108090000672 Annexin A5 Proteins 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 108091033409 CRISPR Proteins 0.000 description 1
- 238000010354 CRISPR gene editing Methods 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 101000760085 Daucus carota 21 kDa protein Proteins 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 101710088172 HTH-type transcriptional regulator RipA Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 239000012098 Lipofectamine RNAiMAX Substances 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- 102000007999 Nuclear Proteins Human genes 0.000 description 1
- 108010089610 Nuclear Proteins Proteins 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 1
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000005775 apoptotic pathway Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 238000003766 bioinformatics method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 208000015799 differentiated thyroid carcinoma Diseases 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- XMBWDFGMSWQBCA-RNFDNDRNSA-M iodine-131(1-) Chemical compound [131I-] XMBWDFGMSWQBCA-RNFDNDRNSA-M 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 230000005937 nuclear translocation Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 239000012474 protein marker Substances 0.000 description 1
- 238000010814 radioimmunoprecipitation assay Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- VSIVTUIKYVGDCX-UHFFFAOYSA-M sodium;4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate Chemical compound [Na+].COC1=CC([N+]([O-])=O)=CC=C1[N+]1=NC(C=2C(=CC(=CC=2)S([O-])(=O)=O)S([O-])(=O)=O)=NN1C1=CC=C([N+]([O-])=O)C=C1 VSIVTUIKYVGDCX-UHFFFAOYSA-M 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000004895 subcellular structure Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000007492 two-way ANOVA Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/04—Antineoplastic agents specific for metastasis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Oncology (AREA)
- Epidemiology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111068922.2A CN113797219B (zh) | 2021-09-13 | 2021-09-13 | Xpr1抑制剂在制备抑制甲状腺癌细胞迁移和/或增殖的产品的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111068922.2A CN113797219B (zh) | 2021-09-13 | 2021-09-13 | Xpr1抑制剂在制备抑制甲状腺癌细胞迁移和/或增殖的产品的应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113797219A true CN113797219A (zh) | 2021-12-17 |
CN113797219B CN113797219B (zh) | 2023-03-31 |
Family
ID=78940929
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111068922.2A Active CN113797219B (zh) | 2021-09-13 | 2021-09-13 | Xpr1抑制剂在制备抑制甲状腺癌细胞迁移和/或增殖的产品的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113797219B (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009117004A1 (en) * | 2008-03-21 | 2009-09-24 | Applied Genomics Inc. | Reagents and methods for use in head and neck cancer diagnosis, classification and therapy |
CN101627121A (zh) * | 2006-12-08 | 2010-01-13 | 奥斯瑞根公司 | 作为治疗干预的靶标的miRNA调控基因和路径 |
AU2015201151A1 (en) * | 2008-11-17 | 2015-03-26 | Veracyte, Inc. | Methods and compositions of molecular profiling for disease diagnostics |
CN105039523A (zh) * | 2008-11-17 | 2015-11-11 | 威拉赛特公司 | 用于疾病诊断的分子表达谱的方法和组合物 |
EP3034620A1 (en) * | 2014-12-17 | 2016-06-22 | Diaxonhit | Compositions and methods for diagnosing thyroid cancer |
-
2021
- 2021-09-13 CN CN202111068922.2A patent/CN113797219B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101627121A (zh) * | 2006-12-08 | 2010-01-13 | 奥斯瑞根公司 | 作为治疗干预的靶标的miRNA调控基因和路径 |
WO2009117004A1 (en) * | 2008-03-21 | 2009-09-24 | Applied Genomics Inc. | Reagents and methods for use in head and neck cancer diagnosis, classification and therapy |
AU2015201151A1 (en) * | 2008-11-17 | 2015-03-26 | Veracyte, Inc. | Methods and compositions of molecular profiling for disease diagnostics |
CN105039523A (zh) * | 2008-11-17 | 2015-11-11 | 威拉赛特公司 | 用于疾病诊断的分子表达谱的方法和组合物 |
EP3034620A1 (en) * | 2014-12-17 | 2016-06-22 | Diaxonhit | Compositions and methods for diagnosing thyroid cancer |
Non-Patent Citations (2)
Title |
---|
REINER K. MAILER: "Xenotropic and polytropic retrovirus receptor 1 regulates procoagulant platelet polyphosphate", 《BLOOD》 * |
邹华伟: "异嗜性和多变性逆转录病毒受体1 在乳头状", 《浙江大学学报(医学版)》 * |
Also Published As
Publication number | Publication date |
---|---|
CN113797219B (zh) | 2023-03-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2019165695A1 (zh) | 一种敲除GRIN2D基因的CRISPR-Cas9系统及其应用 | |
Van Huffel et al. | Identification of a novel A20-binding inhibitor of nuclear factor-κB activation termed ABIN-2 | |
Huang et al. | Knockdown of SGK1 alleviates the IL‐1β‐induced chondrocyte anabolic and catabolic imbalance by activating FoxO1‐mediated autophagy in human chondrocytes | |
Wang et al. | CASK and its target gene Reelin were co-upregulated in human esophageal carcinoma | |
CN112226510B (zh) | Mtx1基因或其表达产物在制备诊断、预防或治疗肝癌的产品中的应用及相关试剂 | |
Liu et al. | Inhibition of PC cell-derived growth factor (PCDGF)/granulin-epithelin precursor (GEP) decreased cell proliferation and invasion through downregulation of cyclin D and CDK 4 and inactivation of MMP-2 | |
Chen et al. | CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP‐9/PI3K/AKT pathway | |
CN113797219B (zh) | Xpr1抑制剂在制备抑制甲状腺癌细胞迁移和/或增殖的产品的应用 | |
Qin et al. | Relationship between p-cofilin and cisplatin resistance in patients with ovarian cancer and the role of p-cofilin in prognosis | |
CN112831497A (zh) | 一种新型lncRNA及其抑制剂、诊断试剂、药物和应用 | |
Shankland et al. | Cyclin and cyclin-dependent kinase expression in the remnant glomerulus. | |
CN112824540A (zh) | Snx5作为肝癌预后的生物学标志物及其应用 | |
WO2019174110A1 (zh) | Rhcg基因在制备治疗癌症药物及诊断性试剂盒中的应用 | |
Everett et al. | Developmental expression of protein phosphatase 2A in the kidney | |
CN114480631B (zh) | Inpp5f用于制备肝癌组织肿瘤预后、诊断或防治的药物的用途 | |
CN114099641A (zh) | Stat6基因为靶点在制备用于治疗急性肺损伤的药物中的应用 | |
Wysocki et al. | lncRNA DIRC3 regulates invasiveness and insulin-like growth factor signaling in thyroid cancer cells | |
WO2013022927A2 (en) | Treatment of uterine leiomyomata | |
CN109295101A (zh) | 过表达miR-125a-5p慢性病毒载体构建方法及其应用 | |
Tong et al. | USP37 promotes angiogenesis and metastasis in colorectal cancer by facilitating β-catenin stability | |
Wang et al. | HDAC5 inhibits ovarian angiogenesis in dehydroepiandrosterone-induced mouse model of polycystic ovary syndrome | |
Miao et al. | Genetic diagnosis of polycystic kidney disease, Alport syndrome, and thalassemia minor in a large Chinese family | |
CN113101368B (zh) | Slc7a8在食管鳞癌辅助诊断、癌前预警和靶向治疗中的应用 | |
CN116790759B (zh) | Plec在上皮性卵巢癌早期诊断及治疗中的应用 | |
CN115896283A (zh) | Crot在制备耐紫杉醇卵巢癌诊断试剂和治疗药物中的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240802 Address after: Building 3, No. 188 Changzu Road, Daqiao Town, Nanhu District, Jiaxing City, Zhejiang Province 314000, First and Second Floors 201 Patentee after: Zhejiang Kangfulong Instrument Co.,Ltd. Country or region after: China Address before: 325000 South Baixiang street, Ouhai District, Wenzhou City, Zhejiang Province Patentee before: THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL University Country or region before: China |