CN113797124B

CN113797124B - Preparation method of yeast bifidus skin care stock solution for repairing and relieving sensitive muscles

Info

Publication number: CN113797124B
Application number: CN202111126964.7A
Authority: CN
Inventors: 刘牧青
Original assignee: Yishen Cell Biotechnology Shanghai Co ltd
Current assignee: Yishen Cell Biotechnology Shanghai Co ltd
Priority date: 2021-09-26
Filing date: 2021-09-26
Publication date: 2023-02-24
Anticipated expiration: 2041-09-26
Also published as: CN113797124A

Abstract

The invention discloses a preparation method of a yeast bifidus skin care stock solution for repairing and relieving sensitive muscles, which comprises the following steps: firstly, sterilizing and breaking the walls after culturing the secondary fission yeast; regulating the pH value of the solution to 6.2-6.9; then adding zinc sulfate, fully stirring to generate precipitation, and filtering to obtain a yeast lysate without kojic acid; in the second step, naOH is used for adjusting the pH value of the solution; the usage amount of ZnSO4.H2O added in the third step is slightly more than the amount required by theoretical calculation. The preparation method of the invention adopts the extraction mode of kojic acid precipitation, so that the fermented secondary cracking yeast filtrate only contains various vitamins, minerals and amino acids; the precipitation method is used for reacting at a medium pH value at normal temperature, and the structures of various nutrient substances are not damaged; after the kojic acid is removed, the sensitive muscle can be better repaired, and the problems of irritation and excessive metabolism of the kojic acid on barriers are also removed; so that the function of the split yeast stock solution can be obtained by users with thin sensitive muscles or barriers.

Description

Preparation method of yeast bifida skin care stock solution for repairing and relieving sensitive muscles

Technical Field

The invention relates to the technical field of removing impurities by microbial fermentation, in particular to a preparation method of a yeast schizophyllum commune skin care stock solution for repairing and relieving sensitive muscles.

Background

The lysate of the fermentation product of the yeast bifidus is obtained by culturing, inactivating and decomposing bifidobacterium to obtain metabolites, cytoplasmic fragments, cell wall components and polysaccharide complexes, and simultaneously contains abundant vitamins, minerals, amino acids and kojic acid in the fermentation process. Can help skin moisturize, repair barriers, promote metabolism and repair ultraviolet injury, so the skin care cream is often applied to various skin care products. The production of kojic acid is difficult to avoid in the production process of the yeast for second splitting fermentation. Kojic acid can promote cutin metabolism and exfoliation to achieve whitening and even anti-aging effects, but for sensitive muscle users with thinned barriers or local deficiency, the presence of kojic acid can increase stimulation, so that the skin generates red and stabbing pain and other side effects. And hypermetabolizing cutin worsens the condition that sensitive muscle cutin is too thin, bringing the sensitive state into the vicious circle. Therefore, most of sensitive muscles are skin care products which are not suitable for using the yeast bifidus.

Based on the method, the invention designs a preparation method of the yeast bifidus skin care stock solution for repairing and relieving sensitive muscles.

Disclosure of Invention

The invention aims to provide a method for preparing a yeast bifidus skin care stock solution for repairing and relieving sensitive muscles, which aims to solve the problem that the existence of kojic acid can increase stimulation and cause the skin to generate red stabbing pain and other side effects on sensitive muscle users with thinned barriers or locally-deleted barriers proposed in the background art. And the problem that the cutin is excessively metabolized to cause the condition that the cutin of sensitive muscles is too thin to be worsened and cause the sensitive state to enter a vicious circle.

In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of a yeast bifidus skin care stock solution for repairing and relieving sensitive muscles comprises the following steps:

the first step is as follows: sterilizing and breaking the wall after culturing the secondary split yeast;

the second step is that: adjusting the pH value of the solution to 6.2-6.9;

the third step: adding zinc sulfate, stirring thoroughly to precipitate, and filtering to obtain yeast lysate without kojic acid.

As a further variant of the invention, naOH is used to adjust the pH of the solution in the second step.

As a further scheme of the invention, the amount of ZnSO4.H2O added in the third step is slightly more than that required in the theoretical calculation.

Compared with the prior art, the invention has the beneficial effects that: the preparation method of the invention adopts the extraction mode of kojic acid precipitation, so that the fermented secondary fission yeast filtrate only contains various vitamins, minerals and amino acids; the precipitation method is used for reacting at a medium pH value at normal temperature without damaging the structures of various nutrient substances; after the kojic acid is removed, the repair effect on sensitive muscles can be better exerted, and the problems of irritation and excessive metabolism of the kojic acid on barriers can be solved; therefore, the functions brought by the yeast liquid can be obtained by the user with thin sensitive muscle or barrier.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 shows the values of the components of the solution at pH 6.54 for the precipitated divalent zinc ion in accordance with one embodiment of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides the following technical scheme: a preparation method of a yeast bifidus skin care stock solution for repairing and relieving sensitive muscles comprises the following steps:

the first step is as follows: sterilizing and breaking the walls after culturing the secondary fission yeast;

the second step is that: adjusting the pH value of the solution to 6.2-6.9;

Specifically, in the second step, naOH is used to adjust the pH of the solution. Sterilizing and breaking the wall after culturing the secondary fission yeast. Taking a certain fermentation liquor, using NaOH to adjust the pH value of the solution, and because Zn ions are zwitter ions and are easy to reversely dissolve in the solution under the conditions of overhigh pH value and overlow pH value, the pH value needs to be adjusted to be within the neutral range of 6.2-6.9 so as to enable chelation to occur, and the generated zinc kojiate is separated out and is not dissolved back.

Referring to fig. 1, the precipitated divalent zinc ions reached ionic equilibrium at PH 6.54 and began to precipitate and stabilize. Adding slightly excessive ZnSO4.H2O after theoretical calculation, (for example: 7.29g is theoretically required to be added into 300ml of filtrate, and 8.0g is actually added, because the added chelating agent is also crystal solid, and even if the excessive chelating agent does not react, the chelating agent exists as precipitate, and does not pollute the stock solution), fully stirring, and then extracting the filtrate in vacuum, and leaving the precipitate. The extracted solution is the required secondary fission yeast filtrate. The extracted solution is tested by taking a 1% solution, a Shijiajunzhi method (a 1% Fecl3-HCl solution is used as a color developing agent to test the residue of kojic acid in the solution, if no color development exists, the removal of the kojic acid is proved to be in a satisfactory state, if iron ions are developed, the OD500 value is measured, and then the OD500 value is input into a regression equation y =0.0014+0.0832x (the correlation coefficient is 0.9987) to calculate the amount of the kojic acid, then a precipitation method is used for extracting the kojic acid for the second time, the test is free of color development, and the PH value is about 6.2.

Specifically, the usage amount of ZnSO4.H2O added in the third step is slightly more than the required amount calculated theoretically; since the added chelating agent is also a crystalline solid, it is present as a precipitate even if it is excessive and does not react, and does not contaminate the stock solution.

The preparation method of the invention adopts the extraction mode of kojic acid precipitation, so that the fermented secondary fission yeast filtrate only contains various vitamins, minerals and amino acids; the precipitation method is used for reacting at a medium pH value at normal temperature without damaging the structures of various nutrient substances; after the kojic acid is removed, the repair effect on sensitive muscles can be better exerted, and the problems of irritation and excessive metabolism of the kojic acid on barriers can be solved; therefore, the functions brought by the yeast liquid can be obtained by the user with thin sensitive muscle or barrier.

In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A preparation method of a yeast bifidus skin care stock solution for repairing and relieving sensitive muscles is characterized by comprising the following steps:

the second step: adjusting the pH value of the solution to 6.2-6.9;

the third step: adding ZnSO4.H2O, fully stirring to generate precipitate, and filtering to obtain the yeast lysate without kojic acid.

2. The method for preparing a yeast for skin care stock solution for sensitive muscle repair and relaxation according to claim 1, wherein the yeast for skin care stock solution comprises the following steps: in the second step, the pH of the solution is adjusted using NaOH.

3. The method for preparing the yeast bifidus skin care stock solution for sensitive muscle repair and relaxation according to claim 1, wherein the yeast bifidus skin care stock solution comprises the following components: the amount of ZnSO4.H2O used in the third step was 8.0g per 300ml of the filtrate.