CN113795757A - Automatic analysis device and automatic analysis method - Google Patents
Automatic analysis device and automatic analysis method Download PDFInfo
- Publication number
- CN113795757A CN113795757A CN202080009339.1A CN202080009339A CN113795757A CN 113795757 A CN113795757 A CN 113795757A CN 202080009339 A CN202080009339 A CN 202080009339A CN 113795757 A CN113795757 A CN 113795757A
- Authority
- CN
- China
- Prior art keywords
- reagent
- reagents
- pair
- tests
- valid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004458 analytical method Methods 0.000 title claims abstract description 62
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 397
- 238000012360 testing method Methods 0.000 claims abstract description 101
- 239000007788 liquid Substances 0.000 claims abstract description 49
- 239000000523 sample Substances 0.000 claims abstract description 41
- 238000003860 storage Methods 0.000 claims abstract description 38
- 230000007246 mechanism Effects 0.000 claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 238000004364 calculation method Methods 0.000 claims abstract description 19
- 230000008859 change Effects 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 description 25
- 238000005259 measurement Methods 0.000 description 24
- 238000000034 method Methods 0.000 description 20
- 238000010586 diagram Methods 0.000 description 9
- 238000013500 data storage Methods 0.000 description 5
- 238000007726 management method Methods 0.000 description 5
- 230000023555 blood coagulation Effects 0.000 description 4
- 238000000465 moulding Methods 0.000 description 4
- 238000010876 biochemical test Methods 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000013062 quality control Sample Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
Abstract
The invention provides an automatic analysis device and an automatic analysis method, which can change the structure of a reagent (bottle) pair according to the actual use condition after determining the structure of the reagent (bottle) pair. An automatic analyzer includes a dispensing mechanism for dispensing a plurality of reagents, and is characterized by comprising: a reagent probe for dispensing a reagent filled in a reagent container; a liquid level detection unit that detects a liquid level of a reagent via the reagent probe; a calculation unit that calculates a remaining amount of the reagent in the reagent container based on a liquid level height of the reagent detected by the liquid level detection unit; and a storage unit that stores the data calculated by the calculation unit, wherein the calculation unit calculates the number of valid tests for each of the reagent containers based on the calculated remaining amount of each of the plurality of reagents, registers a reagent pair composed of a combination of the plurality of reagents in the storage unit based on the calculated number of valid tests, corrects the number of valid tests for each of the reagent containers according to the usage status of the plurality of reagents after the start of analysis, and re-registers the reagent pair.
Description
Technical Field
The present invention relates to an automatic analysis device and an automatic analysis method for analyzing the amount of a component contained in a sample such as blood or urine.
Background
Examples of a sample test for processing a sample such as blood or urine collected from a patient include biochemical tests, immunological tests, blood coagulation tests, and the like.
For example, in tests for analyzing components such as blood and urine, biochemical tests in which a sample is reacted with a reagent to measure components such as sugars, lipids, proteins, and enzymes, and immunological tests in which antibodies, hormones, tumor markers, and the like generated when bacteria and viruses enter the body are measured by antigen-antibody reactions are known.
In the biochemical test, measurement is generally performed using an automatic biochemical analyzer that mixes a sample and a reagent and measures a change in color due to a chemical reaction by transmitted light, and in the immunoassay test, measurement is generally performed using an immunoassay device that adds an antibody bound to a luminophore to an antigen contained in the sample to cause an antigen-antibody reaction, washes the unbound antibody, and measures the luminescence amount due to the bound antibody.
In addition, in an automatic biochemical analyzer, there is also a method of detecting an antibody contained in a sample using a reagent in which an antibody is immobilized on a latex particle. In addition, in blood coagulation tests, there are items for measuring the time taken until blood coagulation and items for measuring molecular markers involved in blood coagulation reactions by transmitted light.
In order to perform efficient analysis in an automatic analyzer, there is a function of sequentially switching to the next reagent container when there is no reagent. Here, since the reagent mixed with the sample is composed of 2 kinds of reagents in many cases, the reagents are managed for each pair. However, the reagents in pairs are not necessarily at the same timing due to errors in the molding of the reagent containers, errors in the amount of reagent filled, and the like. Further, in a case where the apparatus is stopped suddenly between the first reagent and the second reagent, only one of the reagent pair may be consumed earlier.
As a background art in this field, for example, there is a technology as in patent document 1. Patent document 1 discloses "a system capable of calculating the number of remaining uses per reagent pair, which is a combination of a first reagent bottle and a second reagent bottle, from the number of uses of the first reagent and the number of uses of the second reagent when a plurality of reagents are reacted with a sample to be measured, and selecting a reagent pair to be excluded from reagent pairs used for a reaction with the sample when the number of remaining uses is small".
Documents of the prior art
Patent document
Patent document 1: japanese patent laid-open publication No. 2016-95147
Patent document 2: japanese patent laid-open publication No. 2007-322241
Disclosure of Invention
Problems to be solved by the invention
However, in the method described in patent document 1, the excess reagent cannot be used up, and the reagent may be consumed wastefully. The remaining number of times of use per bottle pair in patent document 1 is a theoretical value calculated by the calculation unit, and for example, when the number of times that can be actually measured differs from the prediction of the number of times of use calculated before the start of analysis due to a molding error of a reagent container, an error of a reagent filling amount, or the like, the amount of reagent that remains without being used up increases.
Further, in the above-mentioned patent document 2, management for each reagent pair is not assumed, and as in the patent document 1, a molding error of the reagent container and an error of the amount of the reagent filled are not taken into consideration, so that there is a problem in the accuracy of calculating the remaining amount of the reagent.
Therefore, an object of the present invention is to provide an automatic analyzer and an automatic analysis method that can change the configuration of a reagent (bottle) pair in accordance with the actual usage situation after the configuration of the reagent (bottle) pair is determined.
Means for solving the problems
In order to solve the above problems, the present invention is an automatic analyzer having a dispensing mechanism for dispensing a plurality of reagents, the automatic analyzer comprising: a reagent probe for dispensing a reagent filled in a reagent container; a liquid level detection unit that detects a liquid level of a reagent via the reagent probe; a calculation unit that calculates a remaining amount of the reagent in the reagent container based on a liquid level height of the reagent detected by the liquid level detection unit; and a storage unit that stores the data calculated by the calculation unit, wherein the calculation unit calculates the number of valid tests for each of the reagent containers based on the calculated remaining amount of each of the plurality of reagents, registers a reagent pair composed of a combination of the plurality of reagents in the storage unit based on the calculated number of valid tests, corrects the number of valid tests for each of the reagent containers according to the usage status of the plurality of reagents after the start of analysis, and re-registers the reagent pair.
The present invention is an automatic analysis method for dispensing a plurality of reagents into sample containers, wherein the number of valid tests for each reagent container in which each of the plurality of reagents is stored is calculated, a reagent pair composed of a combination of the plurality of reagents is determined based on the calculated number of valid tests, and after the start of analysis, the number of valid tests for each reagent container is corrected in accordance with the usage status of the plurality of reagents, and the reagent pair is registered again.
Effects of the invention
According to the present invention, it is possible to provide an automatic analysis apparatus and an automatic analysis method that can change the configuration of a reagent (bottle) pair in accordance with the actual usage situation after the configuration of the reagent (bottle) pair is determined.
This enables the reagent to be used up to the end without unnecessarily consuming the reagent.
Problems, structures, and effects other than those described above will become apparent from the following description of the embodiments.
Drawings
Fig. 1 is a diagram showing a basic configuration of an automatic analyzer according to an embodiment of the present invention.
Fig. 2 is a diagram showing a basic configuration of a reagent liquid level detection mechanism of an automatic analyzer according to an embodiment of the present invention.
Fig. 3 is a diagram showing a reagent pack according to an embodiment of the present invention.
Fig. 4 is a flowchart showing an automatic analysis method (reagent pair registration method) in example 1.
FIG. 5 is a diagram showing an example of a reagent pair in example 1.
FIG. 6A is a diagram showing an example of a reagent pair in example 1.
FIG. 6B is a diagram showing an example of the reagent pair in example 1.
Fig. 7 is a flowchart showing an automatic analysis method (reagent pair re-registration method) in example 1.
FIG. 8 is a diagram showing an example of a reagent pair in example 1.
Fig. 9 is a diagram showing a modification of the calculation of the effective test of the reagent pair in example 1.
Fig. 10 is a flowchart showing an automatic analysis method (reagent pair re-registration method) in example 2.
FIG. 11 is a diagram showing an example of a reagent pair in example 2.
Detailed Description
Hereinafter, embodiments for carrying out the present invention will be described in detail with reference to the drawings. In general, the same reference numerals are given to the components having the same functions in the drawings, and the description thereof may be omitted.
Integrated Structure of apparatus
First, a basic configuration of an automatic analyzer and a flow of analysis based on the basic configuration will be described with reference to fig. 1 and 2. When the sample 2 filled in the sample container 1 is set on the sample disk 3, it is sucked by the sample dispensing mechanism 4 and discharged into the reaction container 5.
The reaction vessel 5 containing the sample is moved to the first reagent dispensing position by the rotation operation of the reaction disk 6, and the first reagent dispensing mechanism 7a dispenses the first reagent 8a for analysis from the first reagent vessel 9a to the reaction vessel 5.
Subsequently, the first reagent stirring mechanism 10a stirs the mixed solution in the reaction container 5. After a predetermined time has elapsed, the second reagent dispensing mechanism 7b dispenses the second reagent 8b for analysis from the second reagent container 9b into the reaction vessel 5. Subsequently, the mixed liquid in the reaction container 5 is stirred by the second reagent stirring mechanism 10 b.
Here, the reaction vessel 5 into which the second reagent 8b is dispensed is the same as the reaction vessel 5 containing the sample 2 and the first reagent 8a described above. The reaction vessel 5 is maintained at a constant temperature, for example, 37 ℃ by circulating the liquid 11 through a constant temperature bath filled in the lower part of the reaction tray 6, thereby promoting the reaction and stabilizing the progress of the reaction.
The series of operations are controlled by the control circuit 21. The amount of the liquid mixture in the reaction well 5 is measured by the transmitted light measuring circuit 22 when the liquid mixture passes through the absorption spectrophotometer 12 in accordance with the rotation of the reaction disk 6. The transmitted light amount data thus obtained is sent to a PC (personal computer) 23, and the concentration of the target component in the sample is calculated by an arithmetic section in the PC23, and the data is stored in a data storage section, and the arithmetic result is displayed on an output section 24. The reaction vessel 5 after the reaction is cleaned by the cleaning mechanism 13 and is repeatedly used in the next reaction.
Here, the reagent containers 9a and 9b are provided in the first reagent storage 14a and the second reagent storage 14b, respectively. As shown in fig. 2, the first reagent dispensing mechanism 7a and the second reagent dispensing mechanism 7b are connected to a control unit (control circuit 21) via a liquid level detection circuit 26, and the remaining amount of the reagent is managed by information from the liquid level detection circuit 26 and displayed on a reagent management screen of the output unit 24.
Fig. 1 shows an example in which the reagent dispensing mechanism and the reagent (storage) library are separately configured, but this configuration may not necessarily be the case. For example, a configuration may be adopted in which a plurality of reagents are dispensed by 1 reagent dispensing mechanism, or a configuration may be adopted in which a plurality of reagents are stored in 1 reagent storage.
Example 1
Next, the remaining amount control of the reagent in the present invention will be described with reference to fig. 3 to 6B. Fig. 3 shows a reagent container 9 used in the present invention and a structure for detecting the liquid level of a reagent. Fig. 4 is a flowchart showing an automatic analysis method (reagent pair registration method) according to the present embodiment.
In the reagent containers 9a and 9b provided in the first reagent storage 14a and the second reagent storage 14b of fig. 1, the reagent is recognized by an instruction from the input unit 25, and the remaining amount is registered. Here, the identification of the reagent and the registration of the remaining amount are described as separate operations, but may be performed together (simultaneously).
As a method of discriminating the reagent, for example, there is a method of reading the individual identifier 16 attached to the reagent pack 9 shown in fig. 3 by the reading units 15a and 15 b. Examples of the individual identifier 16 include a barcode, an RFID, and the like, but are not limited thereto.
Further, there is also a method of manually inputting the reagent pack 9 not having the individual identifier 16 from the operation unit.
The first reagent storage 14a and the second reagent storage 14b perform a rotation operation in response to an instruction from the input unit 25. Thus, the reagent containers 9a and 9b move, and the reagent information given to the individual identifier 16 is read every time the reagent containers pass in front of the first reader 15a and the second reader 15 b.
The reagent information indicates, for example, some or all of the test item name, the bottle code, the reagent type, the size of the reagent container, the expiration date of the reagent, the lot, the serial number, the dose line information, and the like. In addition, even when the individual identifier 16 is not given to the reagent containers 9a and 9b, the positions in the first reagent storage 14a and the second reagent storage 14b can be specified from the input unit 25, and the reagent can be identified by inputting the reagent information.
Next, margin registration is performed. The reagent dispensing mechanism 7 is connected to the liquid level detection circuit 26, and when an instruction for registering the remaining amount of the reagent is received from the input unit 25, the operation of the reagent probe 17 is controlled by the control circuit 21 (fig. 2). The information on the capacitance when the tip of the reagent probe 17 reaches the reagent liquid surface is processed by the liquid surface detection circuit 26, and the calculation unit and the data storage unit in the PC23 calculate and store the reagent liquid surface height from the amount of drop of the reagent probe 17.
In addition, in the PC23, the effective test count for each reagent container is calculated from the reagent liquid level height and the sectional area information of the reagent container 9, stored in the data storage portion, and output to the output portion 24 (step S401 of fig. 4). Here, although the method of detecting the change in the electrostatic capacitance is described in the calculation of the reagent liquid level height, other methods such as a pressure detection method in a pipe to which a probe is connected, a detection method by an optical method, and the like may be used.
Next, when a plurality of reagent containers are provided for the same item and the same reagent type, the priority order for each reagent type is determined (step S402 in fig. 4). Further, the reagent type is a classification of reagents such as a diluent, a first reagent, and a second reagent.
Subsequently, according to the processing of step S403 in fig. 4, reagent pairs are registered in order of higher priority. The priority order determination method includes, but is not limited to, the order of the date and time of opening the reagent (the date and time of first loading the reagent into the apparatus), the order of the expiration date of the reagent, the order of the small amount of remaining reagent, and the order of the small amount of position in the reagent storage.
FIG. 5 shows an example of a reagent pair comprising 2 kinds of reagents. Here, an example is shown in which 2 reagent containers capable of 400 test analyses are provided in the first reagent storage 14a, 5 reagents capable of 130 test analyses are provided in the second reagent storage 14b, 2 first reagents are provided at positions 1 to 2 in the first reagent storage 14a, and 5 second reagents are provided at positions 1 to 5 in the second reagent storage 14 b. Here, the priority order is determined in order of the positions from small to large.
In FIG. 5, the second reagents provided at positions 1 to 4 in the second reagent storage 14b are paired with the first reagent provided at position 1 in the first reagent storage 14 a. In this case, in S501 to S503, the effective test for each reagent pair is calculated as 130 tests. Since the remaining amount of the first reagent consumed in S501 to S503 is 10 tests, the effective test of the reagent pair is 10 tests (S504).
In this case, the second reagent disposed at position 4 in the second reagent reservoir 14b is consumed in the remaining amount of 120 tests in S504, and forms a pair with the reagent disposed at position 2 in the first reagent reservoir 14a (S505). Then, the reagent at the position 5 in the second reagent storage 14b is paired with the reagent at the position 2 in the first reagent storage 14a as in S506.
Here, when there is an error in the cross-sectional area information of the reagent container registered in advance due to a molding error of the reagent container or the like, the number of valid tests per reagent container registered at the time of reagent registration may deviate from the number of tests that can be actually analyzed. Regarding the reagent container 9 of fig. 3, the effective test number per reagent container is expressed by the formula (1) using the sectional area 18 and the height 19 from the bottom of the reagent container to the liquid surface.
[ equation 1]
For example, when the cross-sectional area of the reagent container of the first reagent is smaller than 10% of the pre-registered cross-sectional area information and the cross-sectional area of the reagent container of the second reagent matches the pre-registered cross-sectional area information, the number of valid tests at the time of registration of the remaining amount of the reagent deviates from the actual number of measurable tests. The remaining amount of reagent in this case will be described with reference to fig. 6A. With respect to the first reagent, at the time point when the reagent pair is registered, the cross-sectional area registered in advance is 10% more than the actual cross-sectional area, and therefore, the number of predicted valid tests is apparently overestimated.
Here, when the liquid level height is measured every time the measurement is performed and the number of effective tests is updated, the number of effective tests decreases at a rate 10% more than the number of initial predicted tests, and the apparent number of tests in the case of actually performing 130 tests decreases to 143 tests (S601 to S602).
On the other hand, the second reagent is consumed on the assumption of the number of tests when the remaining amount of the reagent is registered. In this case, the number of effective tests of the reagent set at the position 1 in the first reagent storage 14a before the reagent set at the position 3 in the second reagent storage 14b is used up is 0. In this case, the remaining reagent at the position 3 in the second reagent storage 14b newly forms a pair with the reagent at the position 2 in the first reagent storage 14a (S604), and the reagents at the positions 4 and 5 in the first reagent storage 14a also form a pair with the reagent at the position 2 in the first reagent storage 14a (S605 and S606).
This is schematically shown in fig. 6B. When the remaining amounts of reagents are registered, the pairs shown in S611 to S616 are formed, but as a result of reflecting the actual state of analysis, the pair in S614 does not exist, and the pair in S617 is registered instead.
However, in the analyzer, a dose line is created by performing measurement (hereinafter, also referred to as calibration) of a standard sample having a known concentration, and the concentration is calculated by comparing the measurement result of the sample having an unknown concentration with the dose line. For this reason, a dose line is required for each reagent pair. Before a sample to be tested is measured, a measurement accuracy control sample is required to confirm whether or not the conditions of the apparatus and the reagent are problematic.
Therefore, in the automatic analyzer of the present invention, it is checked in advance whether a dose line is registered for each reagent pair or a quality control sample is measured (S404 in fig. 4), and if the measurement result does not exist, the measurement is recommended (executed) (S405 in fig. 4).
Furthermore, the fabrication of the dose line does not necessarily need to be performed for each reagent pair. That is, in the example of fig. 5, when the calibration is performed in the pair of S501, the calibration result of S501 can be applied before using the pairs of S502 to S505.
However, as described above, the number of effective tests of the remaining reagent amount deviates from the actual number of measurable tests, and when the structure of the reagent pair is changed when continuously performing the analysis, the measurement of the dose line and the quality control sample of the reagent pair that is not actually used is performed, resulting in wasteful consumption of the reagent.
The flow of this example in which the number of remaining tests of the reagent is appropriately corrected and the reagent pairs are registered again will be described with reference to fig. 7 and 8.
First, when an instruction for measurement is received from the input unit 25 after a reagent pair is registered by the remaining reagent amount registration (S701 in fig. 7), the control circuit 21 controls various mechanisms to start an analysis operation as described in "overall configuration of apparatus" (S702 in fig. 7).
Thereafter, each time the reagent is aspirated, the first reagent dispensing mechanism 7a and the second reagent dispensing mechanism 7b store the liquid level of the reagent from the inner bottom of the reagent container (reference numeral 19 in fig. 3) and the actual number of analyses in the storage unit of the PC23, and display the remaining amount of the reagent on the reagent management screen of the output unit 24.
Thereafter, the rate of deviation (error rate of the number of measurements) between the number of effective tests of the reagent at the time of registration of the reagent and the actual number of times of aspiration is calculated, the number of effective tests is corrected, and re-registration of reagent pairs is performed in descending order of priority (S703 to S705 in fig. 7). The error rate of the number of measurements is calculated by equation (2).
[ formula 2]
Here, in S801 in fig. 8, it is assumed that, when analysis of 130 tests is actually performed, 143 tests are reduced in the data storage unit of the PC 23. In this case, when the error rate of the number of measurements is calculated by the following formula (2), the error rate of the number of measurements can be calculated to be 10%.
Next, the number of valid trials stored in the storage unit of the PC23 is corrected by the formula (3) using the error rate calculated by the formula (2) and reflected in the remaining amount management (S802 to S805 in fig. 8).
[ formula 3]
Preferably, the reagent pair is registered again and then displayed on the output unit 24. In particular, when the configuration of the reagent pair is different from the configuration of the reagent remaining amount registration, the reagent pair is notified of the update (S706), and execution of calibration and measurement of control are recommended (executed) as necessary (S707).
As described above, the automatic analyzer according to the present embodiment includes: a reagent dispensing mechanism 7 for dispensing a plurality of reagents, comprising: a reagent probe 17 for dispensing a reagent filled in the reagent container 9; a liquid level detection unit (liquid level detection circuit 26) that detects the liquid level of the reagent via the reagent probe 17; a calculation unit that calculates the remaining amount of the reagent in the reagent container 9 based on the liquid level height of the reagent detected by the liquid level detection means (liquid level detection circuit 26); and a storage unit for storing the data calculated by the calculation unit, wherein the calculation unit calculates the number of valid tests for each reagent container 9 based on the calculated remaining amount of each of the plurality of reagents, registers a reagent pair composed of a combination of the plurality of reagents in the storage unit based on the calculated number of valid tests, corrects the number of valid tests for each reagent container 9 according to the usage status of the plurality of reagents after the start of analysis, and registers the reagent pair again.
Further, the cross-sectional area 18 of each reagent container 9 is calculated based on the usage status of a plurality of reagents, the number of valid tests of each reagent container 9 is corrected based on the calculated cross-sectional area 18, and the reagent pair is registered again.
In addition, the error rate is calculated as the rate of deviation between the number of effective tests of the reagent at the time of registration and the actual reagent consumption, and the number of effective tests is corrected based on the error rate.
In the present embodiment, a method of calculating the error rate of the number of measurements and changing the reagent pair when the second reagent is not present and the reagent pair is transferred to the next pair has been described, but the method of calculating the error rate of the number of measurements and the timing of performing the correction are not necessarily limited to the above.
In addition, as the error rate of the number of measurements, the number of tests actually measured when the first reagent container 9a is used up and the number of reductions in the number of effective tests calculated at the time of registration of the remaining amount of reagent and stored in the PC23 are compared in the formula (2), but it may be considered that the number of analyses is divided every time.
That is, it is preferable that 1 test of the reagent is consumed for every 1 dispensing, but the 1 test amount is not necessarily reduced due to an error in the cross-sectional area 18 and a height variation caused by liquid surface detection such as a fluctuation of the liquid surface. Therefore, the error rate may be calculated by counting the increase or decrease in the number of effective tests and the number of times, and considering that errors may occur the same number of times (fig. 9).
In order to change the reagent pair early, after a predetermined number of tests of reagents have been consumed, the error rate of the number of measurements can be calculated from the actual number of measurements and the number of reductions in the number of valid tests stored in the PC 23. If the number of tests at this time is reduced, the timing of updating the reagent pair can be advanced.
However, in reality, when the number of tests to be integrated to some extent or more is consumed, the calculation is performed, and the variation in the liquid level detection height can be calculated with a more accurate error rate. Therefore, if the number of trials until the calculation of the effective trial correction can be input from the input unit 25, the user can correct the effective trial at an arbitrary timing.
Further, there are also a method of automatically confirming the remaining amount of reagent at the time of starting the operation and correcting the number of effective tests, a method of correcting the effective tests when the user registers the remaining amount of reagent at an arbitrary timing, and the like.
Further, when the configuration of the reagent pair is changed by re-registration of the reagent pair, the output unit 24 or a not-shown notification means may be configured to notify that the change has occurred.
As described above, according to the present embodiment, after the configuration of the reagent (bottle) pair is determined, the configuration of the reagent (bottle) pair can be changed in accordance with the actual usage situation, and the reagent can be used up to the end without unnecessarily consuming the reagent.
Example 2
Another embodiment of the present invention will be described with reference to fig. 10. Since the basic flow of correcting the number of tests by analyzing and reflecting the usage status based on the calculation of the number of valid tests for each reagent container is the same as in example 1 (fig. 4), detailed description is omitted and only different points will be described.
In example 1, the error rate of the effective number of tests of the reagent is calculated by detecting the reagent liquid level height every time the reagent probe 17 comes into contact with the reagent liquid level at the time of analysis and updating the effective number of tests, and the re-registration of the reagent pair is performed, but in example 2, the error of the cross-sectional area of the reagent container is corrected.
For example, when the number of actually used tests is small relative to the number of effective tests at the time of registration of a reagent pair, the cross-sectional area of the preliminary reagent container is different from the cross-sectional area of registration. The sectional area can be calculated by the formula (4) using the liquid level height of the reagent from the inner bottom of the reagent container (reference numeral 19 of fig. 3) and the actual number of analysis tests.
[ formula 4]
In this case, at the stage of analyzing 130 tests of the bottle of S801 in fig. 8, the actual reagent consumption amount is calculated from the dispensing amount and the number of analysis tests at position 1 in the first reagent reservoir 14a, and divided by the height 19 from the bottom of the reagent container to the liquid surface, whereby the cross-sectional area of the reagent container is calculated and stored as cross-sectional area information in the data storage unit in the PC23 (S1001 in fig. 10). The number of effective tests is corrected using the sectional area information, and reagent pairs are registered again in descending order of priority (S1002 to S1003 in fig. 10).
Example 3
Next, another embodiment relating to the method of correcting the number of effective tests will be described with reference to fig. 11. The overall flow of the remaining amount of reagent control is substantially the same as in example 1, and therefore, a detailed description thereof is omitted. The difference is that in example 1, the liquid level height of the reagent is measured every time the measurement (analysis) is performed, and the number of valid tests is updated, but in this example, the measurement of the liquid level height every time the measurement (analysis) is performed is not performed.
That is, after the registration of the remaining amount of reagent, the number of valid tests is calculated to constitute a reagent pair, but the number of times of analysis is reduced by software counting without reflecting the liquid level height for each analysis in the remaining amount management thereafter. In this case, there is no difference between the actual number of analysis tests and the number of valid tests stored in PC 23.
However, when the apparatus is stopped suddenly due to a power failure or the like until the second reagent is dispensed after the first reagent is dispensed, the number of effective tests of only the first reagent is reduced. For example, after the registration of the remaining amount of reagent, the reagent pair as shown in fig. 5 is registered, the reagent at position 1 in the first reagent storage 14a is consumed in the test 30 times, and the remaining amount of reagent in the case where the apparatus is stopped by the emergency stop before the dispensing of the second reagent is started is schematically shown in fig. 11.
In S1101, since 30 times of tests are dispensed with the reagent at position 1 in the first reagent storage 14a, the number of valid tests is 370 tests. However, since the second reagent is not dispensed, the number of effective tests of the reagent at position 1 in the second reagent storage 14b constituting the pair is not changed while the number is maintained at 130. Thereafter, if the analysis is resumed, the reagent at the position 1 in the first reagent reservoir 14a is used up before the reagent at the position 3 in the second reagent reservoir 14b is used up, and the first reagent is not used up and 20 test amounts remain.
In order to effectively use up these components, the reagent pair may be reconfigured by adding the process of S1107 instead of performing the process of S1104 as intended.
That is, by automatically re-registering the reagent pair before the measurement is restarted after the device is temporarily stopped, even when only one of the reagents constituting the pair is consumed, the reagent pair can be efficiently used up by re-registering the reagent pair in accordance with the state of the analysis.
The present invention is not limited to the above-described embodiments, and various modifications are possible. For example, the above-described embodiments are described in detail to help understanding of the present invention, and are not necessarily limited to having all the configurations described. In addition, a part of the structure of one embodiment may be replaced with the structure of another embodiment, and the structure of another embodiment may be added to the structure of one embodiment. In addition, a part of the configuration of each embodiment can be added, deleted, or replaced with another configuration.
Description of the reference numerals
1 … sample container
2 … test sample
3 … sample plate
4 … sample dispensing mechanism
5 … reaction vessel
6 … reaction disc
7 … reagent dispensing mechanism
7a … first reagent dispensing mechanism
7b … second reagent dispensing mechanism
8a … first reagent
8b … second reagent
9 … reagent container
9a … first reagent container
9b … second reagent Container
10a … first reagent rabbling mechanism
10b … second reagent rabbling mechanism
11 … thermostatic bath circulating liquid
12 … absorption photometer
13 … cleaning mechanism
14a … first reagent library
14b … second reagent library
15a … first reading part
15b … second reading unit
16 … individual identifier
17 … reagent probe
18 … cross-sectional area (of reagent vessel 9)
19 … height of bottom to liquid level in reagent container
21 … control circuit
22 … transmitted light measuring circuit
23 … PC (personal computer)
24 … output part
25 … input part
26 … liquid level detection circuit.
Claims (12)
1. An automatic analyzer having a dispensing mechanism for dispensing a plurality of reagents, comprising:
a reagent probe that dispenses a reagent filled in a reagent container;
a liquid level detection unit that detects a liquid level of a reagent via the reagent probe;
a calculation unit that calculates the remaining amount of the reagent in the reagent container based on the liquid level height of the reagent detected by the liquid level detection means; and
a storage unit for storing the data calculated by the calculation unit,
the calculation unit calculates the number of valid tests for each of the reagent containers based on the calculated remaining amount of each of the plurality of reagents, registers a reagent pair composed of a combination of the plurality of reagents in the storage unit based on the calculated number of valid tests, corrects the number of valid tests for each of the reagent containers according to the usage status of the plurality of reagents after the start of analysis, and registers the reagent pair again.
2. The automatic analysis device according to claim 1,
calculating a sectional area of each of the reagent containers based on the usage of the plurality of reagents,
and correcting the number of valid tests for each of the reagent containers based on the calculated cross-sectional area, and registering the reagent pairs again.
3. The automatic analysis device according to claim 1,
calculating the deviation rate of the effective test number of the reagent to the registered reagent consumption and the actual reagent consumption as an error rate,
correcting the number of valid trials according to the error rate.
4. The automatic analysis device according to claim 1,
after the start of the analysis, the number of valid tests for each of the reagent containers is corrected in accordance with the number of analyses counted down by the software, and the reagent pairs are registered again.
5. The automatic analysis device according to claim 1,
the automatic analyzer comprises: and an input unit capable of inputting the number of analyses until the number of valid tests is corrected.
6. The automatic analysis device according to claim 1,
when the structure of the reagent pair is changed by re-registration of the reagent pair, a notification is made that the change has occurred.
7. An automatic analysis method for dispensing a plurality of reagents into a sample container,
calculating the number of valid tests for each reagent container containing each of the plurality of reagents,
determining a reagent pair composed of a combination of the plurality of reagents based on the calculated effective test number,
after the start of the analysis, the number of valid tests for each of the reagent containers is corrected in accordance with the usage status of the plurality of reagents, and the reagent pair is registered again.
8. The automated analysis method according to claim 7,
calculating a sectional area of each of the reagent containers based on the usage of the plurality of reagents,
correcting the number of valid tests for each of the reagent containers based on the calculated cross-sectional area,
the reagent pairs were again registered.
9. The automated analysis method according to claim 7,
calculating the deviation rate of the effective test number of the reagent to the registered reagent consumption and the actual reagent consumption as an error rate,
correcting the number of valid trials according to the error rate.
10. The automated analysis method according to claim 7,
after the start of the analysis, the number of valid tests for each of the reagent containers is corrected in accordance with the number of analyses counted down by the software, and the reagent pairs are registered again.
11. The automated analysis method according to claim 7,
the number of analyses until the number of valid tests is corrected is set in advance.
12. The automated analysis method according to claim 7,
when the structure of the reagent pair is changed by re-registration of the reagent pair, a notification is made that the change has occurred.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019-039248 | 2019-03-05 | ||
| JP2019039248 | 2019-03-05 | ||
| PCT/JP2020/004086 WO2020179317A1 (en) | 2019-03-05 | 2020-02-04 | Automatic analysis device and automatic analysis method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113795757A true CN113795757A (en) | 2021-12-14 |
Family
ID=72337557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080009339.1A Pending CN113795757A (en) | 2019-03-05 | 2020-02-04 | Automatic analysis device and automatic analysis method |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP7204878B2 (en) |
| CN (1) | CN113795757A (en) |
| WO (1) | WO2020179317A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023090017A1 (en) * | 2021-11-22 | 2023-05-25 | 株式会社島津製作所 | Analysis device and method |
| WO2024033534A1 (en) | 2022-08-11 | 2024-02-15 | Preomics Gmbh | Fractionation of proteins for proteomics |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1153305A (en) * | 1995-07-11 | 1997-07-02 | 株式会社日立制作所 | Method and apparatus for management of reagent |
| CN101126762A (en) * | 2006-08-18 | 2008-02-20 | 希森美康株式会社 | Sample analyzer |
| US20100115463A1 (en) * | 2008-10-31 | 2010-05-06 | Sysmex Corporation | Sample analyzer, reagent information displaying method and computer program product |
| CN101726610A (en) * | 2008-10-27 | 2010-06-09 | 希森美康株式会社 | Sample analyzer |
| JP2011102705A (en) * | 2009-11-10 | 2011-05-26 | Hitachi High-Technologies Corp | Automatic analysis apparatus |
| WO2013150978A1 (en) * | 2012-04-06 | 2013-10-10 | 株式会社日立ハイテクノロジーズ | Automatic analysis device |
| JP2014198934A (en) * | 2013-03-29 | 2014-10-23 | 株式会社クボタ | Hose guide structure for work machine |
| US20150064795A1 (en) * | 2013-08-30 | 2015-03-05 | Sysmex Corporation | Sample analyzer and sample analyzing method |
| WO2018051672A1 (en) * | 2016-09-16 | 2018-03-22 | 株式会社 日立ハイテクノロジーズ | Automated analyzer, automated analysis system, and method for displaying reagent list |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6017362A (en) * | 1983-07-11 | 1985-01-29 | Nippon Tectron Co Ltd | Automatic analyzing device |
| JP2003294774A (en) | 2002-04-01 | 2003-10-15 | Olympus Optical Co Ltd | Automatic analyzer |
| JP4966913B2 (en) * | 2007-05-15 | 2012-07-04 | 株式会社日立ハイテクノロジーズ | Liquid dispensing device |
| JP6660861B2 (en) | 2016-09-26 | 2020-03-11 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
-
2020
- 2020-02-04 CN CN202080009339.1A patent/CN113795757A/en active Pending
- 2020-02-04 WO PCT/JP2020/004086 patent/WO2020179317A1/en active Application Filing
- 2020-02-04 JP JP2021503471A patent/JP7204878B2/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1153305A (en) * | 1995-07-11 | 1997-07-02 | 株式会社日立制作所 | Method and apparatus for management of reagent |
| CN101126762A (en) * | 2006-08-18 | 2008-02-20 | 希森美康株式会社 | Sample analyzer |
| CN101726610A (en) * | 2008-10-27 | 2010-06-09 | 希森美康株式会社 | Sample analyzer |
| US20100115463A1 (en) * | 2008-10-31 | 2010-05-06 | Sysmex Corporation | Sample analyzer, reagent information displaying method and computer program product |
| JP2011102705A (en) * | 2009-11-10 | 2011-05-26 | Hitachi High-Technologies Corp | Automatic analysis apparatus |
| WO2013150978A1 (en) * | 2012-04-06 | 2013-10-10 | 株式会社日立ハイテクノロジーズ | Automatic analysis device |
| JP2014198934A (en) * | 2013-03-29 | 2014-10-23 | 株式会社クボタ | Hose guide structure for work machine |
| US20150064795A1 (en) * | 2013-08-30 | 2015-03-05 | Sysmex Corporation | Sample analyzer and sample analyzing method |
| WO2018051672A1 (en) * | 2016-09-16 | 2018-03-22 | 株式会社 日立ハイテクノロジーズ | Automated analyzer, automated analysis system, and method for displaying reagent list |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020179317A1 (en) | 2020-09-10 |
| JP7204878B2 (en) | 2023-01-16 |
| JPWO2020179317A1 (en) | 2021-12-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7193564B2 (en) | Automatic analysis device and automatic analysis method | |
| JP6653375B2 (en) | Automatic analyzer | |
| JP3990944B2 (en) | Automatic analyzer | |
| US8535607B2 (en) | Sample analyzer | |
| JP6873295B2 (en) | Automatic analyzer | |
| US20090292494A1 (en) | Analyzer | |
| CN113795757A (en) | Automatic analysis device and automatic analysis method | |
| EP2009448B1 (en) | Automatic analyzing apparatus and quality control method for analysis supporting liquid in the same | |
| JP4676689B2 (en) | Improved fluid measurement in reaction vessels used with clinical analyzers. | |
| WO2022176295A1 (en) | Automatic analysis device and method for controlling automatic analysis device | |
| CN117795345A (en) | Automatic analysis device and automatic analysis system | |
| WO2021157333A1 (en) | Automated analyzer | |
| JP7472360B2 (en) | Automated Analysis Method | |
| US20220291247A1 (en) | Walk-away time visualization methods and systems | |
| JP2019100894A (en) | Automatic analysis device and automatic analysis method | |
| US20230152343A1 (en) | Laboratory instrument | |
| US20230194556A1 (en) | Automatic analyzer and maintenance method for automatic analyzer | |
| JP4871761B2 (en) | Biological sample analysis method and automatic analyzer | |
| JP2023127333A (en) | Automatic analysis device, program, storage medium, and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |