CN113789321A - Rose bio-enzyme extraction process - Google Patents
Rose bio-enzyme extraction process Download PDFInfo
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- CN113789321A CN113789321A CN202111119480.XA CN202111119480A CN113789321A CN 113789321 A CN113789321 A CN 113789321A CN 202111119480 A CN202111119480 A CN 202111119480A CN 113789321 A CN113789321 A CN 113789321A
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- 241000220317 Rosa Species 0.000 title claims abstract description 55
- 238000000605 extraction Methods 0.000 title claims abstract description 37
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 60
- 238000005360 mashing Methods 0.000 claims abstract description 46
- 238000012360 testing method Methods 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 238000007710 freezing Methods 0.000 claims abstract description 16
- 230000008014 freezing Effects 0.000 claims abstract description 16
- 230000007246 mechanism Effects 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 5
- 239000006285 cell suspension Substances 0.000 claims abstract description 4
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 12
- 230000008569 process Effects 0.000 claims description 11
- 239000004744 fabric Substances 0.000 claims description 10
- 238000001704 evaporation Methods 0.000 claims description 3
- 230000008020 evaporation Effects 0.000 claims description 3
- 238000001125 extrusion Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 238000010257 thawing Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 6
- 238000004544 sputter deposition Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 3
- 241000109329 Rosa xanthina Species 0.000 description 2
- 235000004789 Rosa xanthina Nutrition 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000019719 rose oil Nutrition 0.000 description 2
- 239000010666 rose oil Substances 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- 238000001665 trituration Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009990 desizing Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
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- 238000013508 migration Methods 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C1/00—Crushing or disintegrating by reciprocating members
- B02C1/005—Crushing or disintegrating by reciprocating members hydraulically or pneumatically operated
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C1/00—Crushing or disintegrating by reciprocating members
- B02C1/14—Stamping mills
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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Abstract
The invention provides a rose bio-enzyme extraction process. The rose bio-enzyme extraction process comprises the following steps: s1: taking out the rose petals and cleaning to obtain a rose petal raw material; s2: the obtained rose petal raw material is placed into a mashing mechanism for mashing treatment, the mashed crushed material and juice are filtered, and the filtered juice is collected and filled into a transparent test tube; s3: then putting the transparent test tube filled with the juice in the S2 into an ultrasonic processor for ultrasonic treatment to enable cell suspension in the juice to vibrate rapidly; s4: cooling the juice sonicated in S3; s5: and putting the juice cooled in the S4 into a freezer for freezing. The rose bio-enzyme extraction process provided by the invention has the advantages of accelerating the extraction speed, improving the raw material mashing effect and preventing juice sputtering.
Description
Technical Field
The invention relates to the technical field of biological enzyme, and particularly relates to a rose biological enzyme extraction process.
Background
The biological enzyme is an organic matter which is generated by living cells and has a catalytic action, most of the biological enzyme is protein, and the least of the biological enzyme is RNA, the manufacturing and application fields of the biological enzyme are gradually expanded, the biological enzyme is more and more mature to be applied in the textile industry, and the biological enzyme is widely applied in various fields of textile dyeing and finishing from the desizing of cotton fabrics and the degumming of silk in the past.
Roses are deciduous shrubs of rosaceous plants, rosaceous plants and rosaceous plants, can be used as ornamental flowers and economic crops, and are mainly embodied in the fact that flowers of the roses can be used in the food industry and as main raw materials for extracting essence rose oil, and the rose oil is applied to the industries of cosmetics, foods, fine chemicals and the like.
However, when the traditional rose bio-enzyme extraction process is used, it is found that when the preparation stage in the early stage begins, rose leaves need to be mashed to obtain tissue fluid of rose, the traditional mashing method mostly adopts a stone mortar as a container and adopts a manual hand-held mashing rod to perform long-time mashing operation, or mechanically drives the mashing rod to move up and down to smash the rose leaves, the former needs to consume a long mashing time and more manpower, the extraction efficiency of the whole bio-enzyme is slow, and the latter can make up a part of the defects of manual mashing, but when the whole thicker mashing rod is used to smash, it is found that because the mashing rod can only move up and down to smash, the middle part of the raw material of the rose leaves can be smashed more fully, but the raw material on the periphery of the mashing rod is difficult to smash fully, thus reducing the trituration effect and, moreover, both modes may cause the splashing of the juice during trituration, resulting in a lower extraction rate of the juice.
Therefore, there is a need to provide a new extraction process of rose bio-enzyme to solve the above technical problems.
Disclosure of Invention
The invention aims to provide a rose bio-enzyme extraction process which can improve the raw material mashing effect and prevent juice sputtering while accelerating the extraction speed.
In order to solve the technical problems, the extraction process of the rose bio-enzyme provided by the invention comprises the following steps:
s1: taking out the rose petals and cleaning to obtain a rose petal raw material;
s2: the obtained rose petal raw material is placed into a mashing mechanism for mashing treatment, the mashed crushed material and juice are filtered, and the filtered juice is collected and filled into a transparent test tube;
s3: then putting the transparent test tube filled with the juice in the S2 into an ultrasonic processor for ultrasonic treatment to enable cell suspension in the juice to vibrate rapidly;
s4: cooling the juice sonicated in S3;
s5: firstly, putting the juice cooled in the step S4 into a freezer for freezing, taking out the frozen juice after a period of time, unfreezing the juice in a normal-temperature environment, then putting the juice unfrozen at the normal temperature into the freezer for freezing, taking out the juice again after freezing, unfreezing at the normal temperature, repeating the steps repeatedly for many times, and enabling the juice in the test tube to be layered, wherein the bottom of the juice is fine precipitate, the upper part of the juice is clear cell sap tissue, and the cell sap tissue with the clear upper part of the juice is extracted and filled into the test tube, so that the primary purification is completed;
s6: and (4) performing reduced pressure evaporation treatment on the clear cell sap tissue obtained in the step (S5) by using an evaporator, so as to obtain a concentrated biological enzyme solution with high biological enzyme content, and further finishing the extraction process of the biological enzyme.
Preferably, the filtering process in S2 includes the following steps:
a1: taking out the cylinder with two open ends, fixedly installing a funnel at the bottom end of the cylinder, and then clamping the cylinder in the air to separate the cylinder from the ground;
a2: filter cloth is put into the cylinder, and a transparent test tube is arranged below the funnel, so that the bottom end of the funnel is slightly higher than the transparent test tube;
a3: the crushed aggregates and the juice are poured into the cylinder and stay on the filter cloth, then the crushed aggregates are extruded by the pressure plate, and in the extrusion process, the juice can drip down through the filter cloth and flow into the transparent test tube through the funnel until the collection is finished.
Preferably, in S5, the freezing environment is-20 ℃, and the cycle number of the freezing to normal temperature thawing process is four to six.
Preferably, smash mechanism includes the base, the top fixed mounting of base has the frame, the standing groove has been seted up at the top of base, be equipped with in the standing groove and smash the bucket, the top fixed mounting of base has two extension bars, two equal slidable mounting has the linkage plate on the extension bar, smash the sliding sleeve on the bucket and be equipped with the cover, two one side that the linkage plate is close to each other all with the outer wall fixed connection who hides the cover, two all the cover is equipped with the reset spring on the extension bar, two the top of reset spring all with correspond linkage plate fixed connection, two the bottom of reset spring all with base fixed connection, the top that hides the cover is equipped with the apron, the below of apron is located the disc in the cover, the bottom fixed mounting of disc has a plurality of pole of smashing, the bottom of pole extends to smash in the bucket, the top fixed mounting of apron has the mounting bracket, it installs the pivot to rotate on the mounting bracket, the bottom of pivot extends to the below of apron and with disc fixed connection, the pivot with the apron rotates the connection, the top fixed mounting of frame has two cylinders, two the output shaft of cylinder all extends to in the frame and with apron fixed connection, the output shaft of cylinder with the top swing joint of frame.
Preferably, the fixed cover in the pivot is equipped with the snap ring, a plurality of restricted slots have been seted up on the outer lane of snap ring, one side slidable mounting of mounting bracket has the restricted pole, the one end of restricted pole extends to corresponding in the restricted slot.
Preferably, fixed cover is equipped with the go-between on the restriction pole, slidable mounting has two branches, two on the mounting bracket branch is close to the one end of pivot all with go-between fixed connection, the cover is equipped with tight spring on the restriction pole, tight spring one end with go-between fixed connection, tight spring the other end with one side inner wall fixed connection that the mounting bracket corresponds, just the restriction pole is kept away from the one end fixed mounting of pivot has the pull ring.
Preferably, the top ends of the two sliding connection rods are fixedly provided with contact blocks, and the two contact blocks are respectively matched with the two connecting plates.
Preferably, a sealing ring is fixedly mounted at the top of the shielding sleeve, and the sealing ring is in contact with the bottom of the cover plate.
Preferably, the bottom of apron has seted up the annular, slidable mounting has two slide bars in the annular, the bottom of slide bar extend to the below of apron and with disc fixed connection.
Preferably, two movable holes are formed in the top of the frame, balls are inlaid on the inner walls of the movable holes, and the output shafts of the two cylinders penetrate through the corresponding movable holes and are in contact with the corresponding balls.
Compared with the related technology, the extraction process of the rose bio-enzyme provided by the invention has the following beneficial effects:
the invention provides a rose bio-enzyme extraction process, which can perform relatively comprehensive mashing work on a rose leaf raw material by utilizing a plurality of uniformly distributed mashing rods, and can change the spatial position of each mashing rod by rotating a rotating shaft, so that the mashing comprehensiveness can be further improved.
Drawings
FIG. 1 is a flow chart of the extraction process of rose bio-enzyme provided by the present invention;
FIG. 2 is an enlarged view of portion A of FIG. 1;
FIG. 3 is an enlarged view of the portion B shown in FIG. 1;
FIG. 4 is a schematic view of the bottom connection structure of the disc and the tamper in the process of extracting the rose bio-enzyme provided by the present invention;
FIG. 5 is a schematic perspective view of a snap ring in the extraction process of rose bio-enzyme according to the present invention.
Reference numbers in the figures: 1. a base; 2. a frame; 3. a mashing barrel; 4. a sliding connection rod; 5. a connector tile; 6. a blocking sleeve; 7. a reset spring; 8. a cover plate; 9. a disc; 10. a tamping rod; 11. a mounting frame; 12. a rotating shaft; 13. a snap ring; 14. defining a slot; 15. a cylinder; 16. a contact block; 17. a rod is defined.
Detailed Description
The invention is further described with reference to the following figures and embodiments.
Please refer to fig. 1, fig. 2, fig. 3, fig. 4 and fig. 5 in combination, wherein fig. 1 is a flow chart of the extraction process of the rose bio-enzyme provided by the present invention; FIG. 2 is an enlarged view of portion A of FIG. 1; FIG. 3 is an enlarged view of the portion B shown in FIG. 1; FIG. 4 is a schematic view of the bottom connection structure of the disc and the tamper in the process of extracting the rose bio-enzyme provided by the present invention; FIG. 5 is a schematic perspective view of a snap ring in the extraction process of rose bio-enzyme according to the present invention. The rose bio-enzyme extraction process comprises the following steps:
s1: taking out the rose petals and cleaning to obtain a rose petal raw material;
s2: the obtained rose petal raw material is placed into a mashing mechanism for mashing treatment, the mashed crushed material and juice are filtered, and the filtered juice is collected and filled into a transparent test tube;
s3: then putting the transparent test tube filled with the juice in the S2 into an ultrasonic processor for ultrasonic treatment to enable cell suspension in the juice to vibrate rapidly;
s4: cooling the juice sonicated in S3;
s5: firstly, putting the juice cooled in the step S4 into a freezer for freezing, taking out the frozen juice after a period of time, unfreezing the juice in a normal-temperature environment, then putting the juice unfrozen at the normal temperature into the freezer for freezing, taking out the juice again after freezing, unfreezing at the normal temperature, repeating the steps repeatedly for many times, and enabling the juice in the test tube to be layered, wherein the bottom of the juice is fine precipitate, the upper part of the juice is clear cell sap tissue, and the cell sap tissue with the clear upper part of the juice is extracted and filled into the test tube, so that the primary purification is completed;
s6: and (4) performing reduced pressure evaporation treatment on the clear cell sap tissue obtained in the step (S5) by using an evaporator, so as to obtain a concentrated biological enzyme solution with high biological enzyme content, and further finishing the extraction process of the biological enzyme.
The filtering process in S2 includes the steps of:
a1: taking out the cylinder with two open ends, fixedly installing a funnel at the bottom end of the cylinder, and then clamping the cylinder in the air to separate the cylinder from the ground;
a2: filter cloth is put into the cylinder, and a transparent test tube is arranged below the funnel, so that the bottom end of the funnel is slightly higher than the transparent test tube;
a3: the crushed aggregates and the juice are poured into the cylinder and stay on the filter cloth, then the crushed aggregates are extruded by the pressure plate, and in the extrusion process, the juice can drip down through the filter cloth and flow into the transparent test tube through the funnel until the collection is finished.
In the S5, the freezing environment is-20 ℃, and the cycle times of the process of freezing to normal temperature and thawing are four times.
The mashing mechanism comprises a base 1, a frame 2 is fixedly mounted at the top of the base 1, a placing groove is formed in the top of the base 1, a mashing barrel 3 is arranged in the placing groove, the placing groove is in a circular design and can form size adaptation with the mashing barrel 3, so that the mashing barrel 3 cannot displace in the mashing barrel, two sliding rods 4 are fixedly mounted at the top of the base 1, two connecting rods 5 are slidably mounted on the sliding rods 4, bearing holes are formed in the two connecting rods 5, linear bearings are slidably sleeved on the two sliding rods 4 and are fixedly connected with the inner walls of the two bearing holes respectively, a shielding sleeve 6 is slidably sleeved on the mashing barrel 3, the inner diameter of the shielding sleeve 6 is the same as the outer diameter of the mashing barrel 3, the shielding sleeve is in contact sliding connection, certain sealing performance can be guaranteed, and one side, close to each other, of the two connecting plates 5 is fixedly connected with the outer wall of the shielding sleeve 6, all the sleeves of the two sliding connecting rods 4 are provided with reset springs 7, the top ends of the two reset springs 7 are fixedly connected with the corresponding connecting plates 5, the bottom ends of the two reset springs 7 are fixedly connected with the base 1, the reset springs 7 can provide a rebounding function for the shielding sleeve 6, when the shielding sleeve 6 is separated from the cover plate 8, the shielding sleeve 6 can rebound to the original position under the action of the elastic force of the reset springs 7, the cover plate 8 is arranged above the shielding sleeve 6, the whole size of the cover plate 8 is larger than the maximum outer diameter of the shielding sleeve 6, so that comprehensive coverage can be realized, a disc 9 positioned in the shielding sleeve 6 is arranged below the cover plate 8, a plurality of tamping rods 10 are fixedly arranged at the bottom of the disc 9, the tamping rods 10 are distributed in an even annular mode, so that the rose raw materials can be mashed thoroughly, and the bottom ends of the tamping rods 10 extend into the mashing barrel 3, the top fixed mounting of apron 8 has mounting bracket 11, it installs pivot 12 to rotate on the mounting bracket 11, the bottom of pivot 12 extends to the below of apron 8 and with 9 fixed connection of disc, pivot 12 with 8 rotate the connection of apron, and pivot 12 plays a connection disc 9 to and can realize the effect of disc 9 pivoted, the top fixed mounting of frame 2 has two cylinders 15, two the output shaft of cylinder 15 all extends to in the frame 2 and with 8 fixed connection of apron, the output shaft of cylinder 15 with the top swing joint of frame 2 utilizes the flexible of 15 output shafts of cylinder, alright realize the up-and-down motion of pole for smash the work smoothly.
Fixed cover is equipped with snap ring 13 in the pivot 12, a plurality of restricted slots 14 have been seted up on the outer lane of snap ring 13, and a plurality of restricted slots 14 are the annular array and distribute, and two adjacent restricted slots 14's interval is less, can adapt to the adjustment work of 9 multi-angles of disc, one side slidable mounting of mounting bracket 11 has restricted pole 17, the one end of restricted pole 17 extends to corresponding in the restricted slot 14, restricted pole 17 plays a card and holds the effect, can fix snap ring 13 in the assigned position to can not hinder pounding work of ram 10.
Fixed cover is equipped with the go-between on the restriction pole 17, slidable mounting has two branches, two on the mounting bracket 11 branch is close to the one end of pivot 12 all with go-between fixed connection, branch can restrict the movement track of restriction pole 17, makes it unrotatable, can only carry out stable horizontal migration, the cover is equipped with tight spring on the restriction pole 17, tight spring one end with go-between fixed connection, tight spring the other end with one side inner wall fixed connection that mounting bracket 11 corresponds, tight spring can enough make it automatic return to in the restriction groove 14 under the restriction pole 17 does not have the external force pulling condition, can provide an elastic pressure for restriction pole 17 again, makes its tight card in restriction groove 14, improves stability, just restriction pole 17 keeps away from the one end fixed mounting of pivot 12 has the pull ring.
Two the equal fixed mounting in top of sliding rod 4 has the conflict piece 16, two conflict piece 16 respectively with two link up board 5 looks adaptation, conflict piece 16's setting can provide a highest rise height for sheltering from cover 6, also enables it and can not take place to break away from with sliding rod 4.
The top fixed mounting that shelters from cover 6 has the sealing ring, the sealing ring with the bottom of apron 8 contacts, and the sealing ring is as a most important sealing member, can improve apron 8 and shelter from the leakproofness between the cover 6 on the one hand, and on the other hand the sealing ring is the rubber material mostly, and it has certain softness itself, when apron 8 descends and contacts with it, can not take place rigid collision, reduces wearing and tearing between the spare part.
The annular has been seted up to apron 8's bottom, slidable mounting has two slide bars in the annular, the bottom of slide bar extends to apron 8 the below and with disc 9 fixed connection, the setting of annular and slide bar can provide a support piece except pivot 12 for disc 9 to reduce the gathering nature gravity that pivot 12 received, improve its life.
Two movable holes are formed in the top of the frame 2, balls are inlaid in the inner walls of the movable holes, the output shafts of the two air cylinders 15 penetrate through the corresponding movable holes and are in contact with the corresponding balls, the movable holes can provide movable channels for the output shafts of the air cylinders 15, the balls in contact with the output shafts of the air cylinders 15 can form contact type supports for the output shafts of the air cylinders, and adverse effects caused by the fact that the length of the output shafts of the air cylinders is long and in the aspect of deflection are reduced.
In the initial state, the output shaft of the cylinder 15 is in a retraction state, the connecting plate 5 is in a contact state with the contact block 16, and in addition, the shielding sleeve 6 and the tamping rod 10 are in a separation state with the mashing barrel 3;
when rose blade raw materials need to be mashed, only need earlier to smash barrel 3 and propose from the standing groove, then take it out from base 1, pour into a certain amount of rose blade wherein afterwards, alright place it in the standing groove again, then start stretching out of cylinder 15 output shaft, alright drive apron 8 descends, thereby can be under the connection of pivot 12, drive disc 9 and stamp rod 10 descend, stamp rod 10 just can get into smash barrel 3 gradually, and before stamp rod 10 contacted rose blade, apron 8 can contact the sealing ring on sheltering from cover 6 earlier, thereby push down it, make it push down and form the coincidence on smashing barrel 3, and reset spring 7 at this moment is in the compression state, like this, can be under the interconnect of apron 8, sheltering from cover 6 and smashing barrel 3, form a comparatively sealed environment of smashing, until after last stamp rod 10 contacted rose blade, the smashing operation is carried out for one time, then the output shaft of the air cylinder 15 is started to retract, the smashing rod 10 is brought back to the original height, the shielding sleeve 6 is also returned to the original position under the resilience action of the reset spring 7, then the output shaft of the air cylinder 15 is continuously started to extend and retract, so that the smashing operation is carried out for one time again, and then the steps are circulated for multiple times, so that the smashing operation of the smashing rod 10 can be completed for multiple times under one state;
after a period of time, the output shaft of the starting cylinder 15 retracts, then the limiting rod 17 is pulled towards the direction far away from the mounting frame 11 and is pulled out of the corresponding limiting groove 14, at the moment, the tightening spring is in a deep compression state, then the rotating shaft 12 is rotated in a small angle, the space position of each tamping rod 10 can be changed, the corresponding limiting groove 14 in the state can correspond to the limiting rod 17, then the limiting rod 17 is loosened, the tightening spring can release the elastic force, the limiting rod 17 is brought into the corresponding limiting groove 14, then the cylinder 15 is reused according to the same steps to perform up-and-down mashing operation, and the position of the tamping rod 10 in the space can be adjusted in a mode that the rotating shaft 12 can be rotated for multiple times subsequently, so that the mashing operation can be performed on the rose leaf raw materials in all directions.
Compared with the related technology, the extraction process of the rose bio-enzyme provided by the invention has the following beneficial effects:
the invention provides a rose bio-enzyme extraction process, which can perform relatively comprehensive mashing work on a rose leaf raw material by utilizing a plurality of uniformly distributed mashing rods 10, and can change the spatial position of each mashing rod 10 by rotating a rotating shaft 12, so that the mashing comprehensiveness can be further improved, and in addition, a relatively sealed mashing environment can be formed by the mutual connection of a cover plate 8, a sealing ring, a shielding sleeve 6 and a mashing barrel 3, so that the situation of splashing of juice can be effectively avoided in the mashing process, and the juice extraction rate is improved.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A rose bio-enzyme extraction process is characterized by comprising the following steps:
s1: taking out the rose petals and cleaning to obtain a rose petal raw material;
s2: the obtained rose petal raw material is placed into a mashing mechanism for mashing treatment, the mashed crushed material and juice are filtered, and the filtered juice is collected and filled into a transparent test tube;
s3: then putting the transparent test tube filled with the juice in the S2 into an ultrasonic processor for ultrasonic treatment to enable cell suspension in the juice to vibrate rapidly;
s4: cooling the juice sonicated in S3;
s5: firstly, putting the juice cooled in the step S4 into a freezer for freezing, taking out the frozen juice after a period of time, unfreezing the juice in a normal-temperature environment, then putting the juice unfrozen at the normal temperature into the freezer for freezing, taking out the juice again after freezing, unfreezing at the normal temperature, repeating the steps repeatedly for many times, and enabling the juice in the test tube to be layered, wherein the bottom of the juice is fine precipitate, the upper part of the juice is clear cell sap tissue, and the cell sap tissue with the clear upper part of the juice is extracted and filled into the test tube, so that the primary purification is completed;
s6: and (4) performing reduced pressure evaporation treatment on the clear cell sap tissue obtained in the step (S5) by using an evaporator, so as to obtain a concentrated biological enzyme solution with high biological enzyme content, and further finishing the extraction process of the biological enzyme.
2. The rose bio-enzyme extraction process according to claim 1, wherein the filtering step in S2 comprises the following steps:
a1: taking out the cylinder with two open ends, fixedly installing a funnel at the bottom end of the cylinder, and then clamping the cylinder in the air to separate the cylinder from the ground;
a2: filter cloth is put into the cylinder, and a transparent test tube is arranged below the funnel, so that the bottom end of the funnel is slightly higher than the transparent test tube;
a3: the crushed aggregates and the juice are poured into the cylinder and stay on the filter cloth, then the crushed aggregates are extruded by the pressure plate, and in the extrusion process, the juice can drip down through the filter cloth and flow into the transparent test tube through the funnel until the collection is finished.
3. The rose bio-enzyme extraction process according to claim 1, wherein in the S5, the freezing environment is-20 ℃, and the cycle number of the process of freezing to normal temperature and thawing is four to six.
4. The extraction process of rose bio-enzyme according to claim 1, wherein the mashing mechanism comprises a base, a frame is fixedly mounted on the top of the base, a placing groove is formed in the top of the base, a mashing barrel is arranged in the placing groove, two sliding connecting rods are fixedly mounted on the top of the base, connecting plates are slidably mounted on the two sliding connecting rods, a shielding sleeve is slidably mounted on the mashing barrel, one side, close to each other, of each of the two connecting plates is fixedly connected with the outer wall of the shielding sleeve, a reset spring is sleeved on each of the two sliding connecting rods, the top ends of the two reset springs are fixedly connected with the corresponding connecting plates, the bottom ends of the two reset springs are fixedly connected with the base, a cover plate is arranged above the shielding sleeve, a disc positioned in the shielding sleeve is arranged below the cover plate, and a plurality of mashing rods are fixedly mounted at the bottom of the disc, the bottom of pounding rod extends to smash to pieces in the bucket, the top fixed mounting of apron has the mounting bracket, rotate on the mounting bracket and install the pivot, the bottom of pivot extends to the below of apron and with disc fixed connection, the pivot with the apron rotates and connects, the top fixed mounting of frame has two cylinders, two the output shaft of cylinder all extends to in the frame and with apron fixed connection, the output shaft of cylinder with the top swing joint of frame.
5. The extraction process of rose bio-enzyme according to claim 4, wherein the rotating shaft is fixedly sleeved with a snap ring, the outer ring of the snap ring is provided with a plurality of limiting grooves, one side of the mounting frame is slidably mounted with a limiting rod, and one end of the limiting rod extends into the corresponding limiting groove.
6. The rose bio-enzyme extraction process according to claim 5, wherein the limiting rod is fixedly sleeved with a connecting ring, the mounting frame is slidably mounted with two support rods, two of the support rods are fixedly connected with the connecting ring at ends close to the rotating shaft, the limiting rod is sleeved with a tightening spring, one end of the tightening spring is fixedly connected with the connecting ring, the other end of the tightening spring is fixedly connected with an inner wall of one side corresponding to the mounting frame, and a pull ring is fixedly mounted at an end of the limiting rod far away from the rotating shaft.
7. The rose bio-enzyme extraction process according to claim 4, wherein the top ends of the two sliding connection rods are fixedly provided with contact blocks, and the two contact blocks are respectively matched with the two connection plates.
8. The rose bio-enzyme extraction process according to claim 4, wherein the top of the shielding sleeve is fixedly provided with a sealing ring, and the sealing ring is in contact with the bottom of the cover plate.
9. The extraction process of rose bio-enzyme according to claim 4, wherein the bottom of the cover plate is provided with a ring groove, two sliding rods are slidably mounted in the ring groove, and the bottom ends of the sliding rods extend to the lower part of the cover plate and are fixedly connected with the disc.
10. The rose bio-enzyme extraction process according to claim 4, wherein the top of the frame is provided with two movable holes, the inner walls of the movable holes are embedded with balls, and the output shafts of the two cylinders penetrate through the corresponding movable holes and are in contact with the corresponding balls.
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| CN202111119480.XA CN113789321A (en) | 2021-09-24 | 2021-09-24 | Rose bio-enzyme extraction process |
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| CN202111119480.XA CN113789321A (en) | 2021-09-24 | 2021-09-24 | Rose bio-enzyme extraction process |
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