CN113786384A - Method for realizing oil/water emulsion with adjustable form based on glyceryl monostearate - Google Patents
Method for realizing oil/water emulsion with adjustable form based on glyceryl monostearate Download PDFInfo
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- CN113786384A CN113786384A CN202110446867.XA CN202110446867A CN113786384A CN 113786384 A CN113786384 A CN 113786384A CN 202110446867 A CN202110446867 A CN 202110446867A CN 113786384 A CN113786384 A CN 113786384A
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- emulsion
- glyceryl monostearate
- oil
- ratio
- oil phase
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- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 title claims abstract description 100
- 239000000839 emulsion Substances 0.000 title claims abstract description 78
- 229940075507 glyceryl monostearate Drugs 0.000 title claims abstract description 49
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 title claims abstract description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims description 19
- 239000003921 oil Substances 0.000 claims abstract description 42
- 235000019198 oils Nutrition 0.000 claims abstract description 42
- 238000003756 stirring Methods 0.000 claims abstract description 17
- 239000007787 solid Substances 0.000 claims abstract description 12
- 238000010438 heat treatment Methods 0.000 claims abstract description 11
- 238000000265 homogenisation Methods 0.000 claims abstract description 10
- 238000001816 cooling Methods 0.000 claims abstract description 9
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 7
- 239000008158 vegetable oil Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 235000012424 soybean oil Nutrition 0.000 claims description 16
- 239000003549 soybean oil Substances 0.000 claims description 16
- 235000020238 sunflower seed Nutrition 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000002425 crystallisation Methods 0.000 abstract description 2
- 230000008025 crystallization Effects 0.000 abstract description 2
- 239000002245 particle Substances 0.000 description 16
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000002105 nanoparticle Substances 0.000 description 6
- 230000007547 defect Effects 0.000 description 3
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000004700 cellular uptake Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000155 melt Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000007779 soft material Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 210000004565 granule cell Anatomy 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000012621 metal-organic framework Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/14—Esters of carboxylic acids, e.g. fatty acid monoglycerides, medium-chain triglycerides, parabens or PEG fatty acid esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/44—Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
Heating glyceryl monostearate and vegetable oil in different proportions until the glyceryl monostearate is fully dissolved and uniformly mixed, keeping stirring, adding pure water at the same temperature, performing high-pressure homogenization treatment, and cooling to obtain the emulsion with adjustable form. The invention controls the crystallization degree of the emulsion drop oil phase by controlling the proportion of the solid glyceryl monostearate and the liquid oil, and then prepares the emulsion with different deformability (hardness).
Description
Technical Field
The invention relates to a technology in the field of food industry, in particular to a method for realizing oil/water emulsion with adjustable form based on glyceryl monostearate.
Background
Cellular uptake of nanoparticles involves the process of deposition and deformation of the particles on the cell membrane surface. The deformability of the particles has a significant influence on their cellular uptake process. Compared with the rigid hard particles which are not deformable, the cell absorption efficiency of the soft substance particles with good deformability is obviously improved. Therefore, how to obtain soft material particles with controllable deformability and design the soft material particles as efficient delivery carriers of functional factors/drug molecules becomes one of the research hotspots in the fields of functional foods and biomedicines.
The existing method for controlling the deformability of the micro-nano particles comprises the technology of preparing hollow nano particles by using a metal organic framework, microgel inside the hollow nano particles and the like. The method can construct nano-particles with different deformability, but the preparation process is more complicated and the cost is high, and the preparation raw materials sometimes relate to the use of synthetic macromolecules, so the method has potential edible toxicity and has no good application prospect.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method for realizing oil/water emulsion with adjustable form based on glyceryl monostearate, which regulates and controls the crystallization degree of an emulsion drop oil phase by controlling the proportion of solid glyceryl monostearate and liquid oil, and then prepares emulsions with different deformability (hardness).
The invention is realized by the following technical scheme:
the invention relates to a method for realizing oil/water emulsion with adjustable form based on glyceryl monostearate, which comprises the steps of heating glyceryl monostearate and vegetable oil in different proportions until the glyceryl monostearate is fully dissolved and uniformly mixed, keeping stirring, adding pure water at the same temperature, carrying out high-pressure homogenization treatment, and cooling to obtain the emulsion with adjustable form.
The vegetable oil is soybean oil or sunflower seed oil.
The heating is preferably carried out in a water bath at 60-90 ℃, and more preferably at 80 ℃ for 30 min.
Stirring at 8000rmp for 1 min;
the high-pressure homogenization treatment is carried out, wherein the pressure is 400bar, and the high-pressure homogenization cycle time is 3 times.
The invention relates to an emulsion prepared by the method, which comprises the following components: glyceryl monostearate and soybean oil or glyceryl monostearate and sunflower seed oil; the content of the compound is any one of the following:
when the ratio of the glyceryl monostearate to the soybean oil is more than 1:12, the emulsion is emulsion drops with solid oil phase;
when the ratio of the glyceryl monostearate to the soybean oil is (1:18 to 1:12), the emulsion is emulsion drops of which the oil phase is semisolid;
thirdly, when the ratio of the glyceryl monostearate to the soybean oil is less than 1:24, the emulsion is emulsion drops with an oil phase in a liquid state;
fourthly, when the ratio of the glycerin monostearate to the sunflower seed oil is more than 1:10, the emulsion is emulsion droplets with solid oil phase;
fifthly, when the proportion of the glyceryl monostearate to the sunflower seed oil is (1:20 to 1:10), the emulsion is emulsion drops with oil phase in a semi-solid state;
when the ratio of the glyceryl monostearate to the sunflower seed oil is less than 1:40, the emulsion is emulsion drops with an oil phase in a liquid state.
Technical effects
The invention integrally solves two main defects of complex preparation process and overhigh production cost in the prior art, can prepare multi-scale emulsion delivery carriers with different deformability rapidly, in a large scale and at low cost, and has wide prospect in embedding and delivering functional nutrients and drug molecules.
Compared with the prior art, the invention prepares stable emulsion with different deformability of emulsion drops by adjusting the proportion of the monoglyceride and the vegetable oil so as to realize obvious difference of delivery effect (or complete granule cell absorption rate).
Drawings
FIG. 1 is a graph showing the appearance of glyceryl monostearate and soybean oil mixed at different mass ratios and heated until the glyceryl monostearate melts and then cooled in examples 1-3;
FIG. 2 is the results of the particle size distribution of examples 1-3, with different ratios of glyceryl monostearate to soy oil emulsion for 0 and 14 days;
FIG. 3 is a graph showing the appearance of glyceryl monostearate and soybean oil mixed at different mass ratios and heated until the glyceryl monostearate melts and then cooled in examples 4-6;
the weight ratio of the glyceryl monostearate to the sunflower seed oil is 1:40, 1:20, 1:10 and 1:5 in sequence from left to right in the figure.
Detailed Description
Example 1
The process for preparing emulsion particles with different deformability by using glyceryl monostearate is as follows:
1) heating 2g glyceryl monostearate and 24g soybean oil in water bath at 80 deg.C for 30min, quickly adding into pure water at the same temperature, and quickly stirring at 80 deg.C with stirring speed of 8000rmp for 1min to obtain coarse emulsion;
2) and (3) quickly homogenizing the coarse emulsion under high pressure, wherein the homogenizing pressure is 400bar, the high-pressure homogenizing cycle times are 3 times, and then cooling to room temperature of 25 ℃ to obtain the emulsion with solid emulsion droplet oil phase and low deformability.
Example 2
The process for preparing emulsion particles with different deformability by using glyceryl monostearate is as follows:
1) heating 2g glyceryl monostearate and 36g soybean oil in water bath at 80 deg.C for 30min, quickly adding into pure water at the same temperature, and quickly stirring at 80 deg.C with stirring speed of 8000rmp for 1min to obtain coarse emulsion;
2) and (3) rapidly carrying out high-pressure homogenization on the coarse emulsion, wherein the homogenization pressure is 400bar, the high-pressure homogenization cycle times are 3 times, and then cooling to room temperature of 25 ℃ to obtain the emulsion with semi-solid emulsion droplet oil phase and medium deformability.
Example 3
The process for preparing emulsion particles with different deformability by using glyceryl monostearate is as follows:
1) heating 2g glyceryl monostearate and 48g soybean oil in water bath at 80 deg.C for 30min, quickly adding into pure water at the same temperature, and quickly stirring at 80 deg.C with stirring speed of 8000rmp for 1min to obtain coarse emulsion;
2) and (3) quickly homogenizing the coarse emulsion under high pressure, wherein the homogenizing pressure is 400bar, the high-pressure homogenizing cycle times are 3 times, and then cooling to room temperature of 25 ℃ to obtain the emulsion with high deformable emulsion droplet oil phase.
Example 4
The process for preparing emulsion particles with different deformability by using glyceryl monostearate is as follows:
1) heating 2g glyceryl monostearate and 20g oleum Helianthi in water bath at 80 deg.C for 30min, quickly adding into pure water at the same temperature, and quickly stirring at 80 deg.C with stirring speed of 8000rmp for 1min to obtain coarse emulsion;
2) and (3) quickly homogenizing the coarse emulsion under high pressure, wherein the homogenizing pressure is 400bar, the high-pressure homogenizing cycle times are 3 times, and then cooling to room temperature of 25 ℃ to obtain the emulsion with solid emulsion droplet oil phase and low deformation.
Example 5
The process for preparing emulsion particles with different deformability by using glyceryl monostearate is as follows:
1) heating 2g glyceryl monostearate and 40g oleum Helianthi in water bath at 80 deg.C for 30min, quickly adding into pure water at the same temperature, and quickly stirring at 80 deg.C with stirring speed of 8000rmp for 1min to obtain coarse emulsion;
2) and (3) quickly homogenizing the coarse emulsion at high pressure, wherein the homogenizing pressure is 400bar, the high-pressure homogenizing cycle times are 3 times, and then cooling to room temperature of 25 ℃ to obtain the emulsion with semi-solid and deformable emulsion droplet oil phase.
Example 6
The process for preparing emulsion particles with different deformability by using glyceryl monostearate is as follows:
1) heating 2g glyceryl monostearate and 80g oleum Helianthi in water bath at 80 deg.C for 30min, quickly adding into pure water at the same temperature, and quickly stirring at 80 deg.C with stirring speed of 8000rmp for 1min to obtain coarse emulsion;
2) and (3) quickly homogenizing the coarse emulsion under high pressure, wherein the homogenizing pressure is 400bar, the high-pressure homogenizing cycle times are 3 times, and then cooling to room temperature of 25 ℃ to obtain the emulsion with high deformable emulsion droplet oil phase.
TABLE 1 mean particle size of glyceryl monostearate/soy oil emulsion for 0 and 14 days
Analysis of the differences between the particle sizes of emulsions standing for different days at the same weight ratio of glyceryl monostearate to soybean oil
Compared with the defects of complex process, high cost and difficult industrialization of the prior art for preparing hollow organic nano particles, filling hydrogel with different hardness in the particles and the like, the method can obtain the emulsion with different deformability by simply mixing monoglyceride with different concentrations and vegetable oil and then homogenizing. The preparation process is simple, the cost of the raw materials is low, and the method has good industrial prospect.
The foregoing embodiments may be modified in many different ways by those skilled in the art without departing from the spirit and scope of the invention, which is defined by the appended claims and all changes that come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (6)
1. A method for realizing oil/water emulsion with adjustable form based on glyceryl monostearate is characterized in that glyceryl monostearate and vegetable oil with different proportions are heated until the glyceryl monostearate is fully dissolved and uniformly mixed, stirring is kept, pure water with the same temperature is added, then high-pressure homogenization treatment is carried out, and emulsion with adjustable form is obtained after cooling;
the vegetable oil is soybean oil or sunflower seed oil.
2. The method of claim 1, wherein said heating is carried out at a temperature of 60-90 ℃.
3. The method for realizing a morphologically tunable oil/water emulsion based on glyceryl monostearate according to claim 1 or 2, wherein said heating, in particular in a 80 ℃ water bath, is carried out for 30 min.
4. A process for the preparation of a morphologically tunable oil/water emulsion according to claim 1, wherein said stirring is carried out at 8000rmp for 1 min.
5. The method of claim 1, wherein the high pressure homogenization treatment is performed at a pressure of 400bar and the number of high pressure homogenization cycles is 3.
6. An emulsion prepared according to any one of claims 1 to 5, characterized in that it comprises the following components: glyceryl monostearate and soybean oil or glyceryl monostearate and sunflower seed oil; the content of the compound is any one of the following:
when the ratio of the glyceryl monostearate to the soybean oil is more than 1:12, the emulsion is emulsion drops with solid oil phase;
when the ratio of the glyceryl monostearate to the soybean oil is 1: 18-1: 12, the emulsion is emulsion drops with oil phase being semisolid;
thirdly, when the ratio of the glyceryl monostearate to the soybean oil is less than 1:24, the emulsion is emulsion drops with an oil phase in a liquid state;
fourthly, when the ratio of the glycerin monostearate to the sunflower seed oil is more than 1:10, the emulsion is emulsion droplets with solid oil phase;
fifthly, when the ratio of the glyceryl monostearate to the sunflower seed oil is 1: 20-1: 10, the emulsion is emulsion drops with oil phase in a semi-solid state;
when the ratio of the glyceryl monostearate to the sunflower seed oil is less than 1:40, the emulsion is emulsion drops with an oil phase in a liquid state.
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| CN202110446867.XA CN113786384A (en) | 2021-04-25 | 2021-04-25 | Method for realizing oil/water emulsion with adjustable form based on glyceryl monostearate |
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| CN202110446867.XA CN113786384A (en) | 2021-04-25 | 2021-04-25 | Method for realizing oil/water emulsion with adjustable form based on glyceryl monostearate |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4443487A (en) * | 1981-02-27 | 1984-04-17 | Lever Brothers Company | Process for producing a spreadable emulsion |
| EP0563593A1 (en) * | 1992-03-02 | 1993-10-06 | Kao Corporation | Oil-in-water type emulsion for whipped cream, powder and whipped cream |
| JPH0799886A (en) * | 1993-09-30 | 1995-04-18 | Snow Brand Milk Prod Co Ltd | Emulsion substitutable for fat, its usage and frozen confectionary using the same |
| US20120034353A1 (en) * | 2010-08-09 | 2012-02-09 | Joerg Eggeling | High Pressure Homogenization In Combination With Functionalized Egg For Production Of Emulsion-Based Food Products |
| CN110215416A (en) * | 2019-06-06 | 2019-09-10 | 浙江大学 | A kind of camellia oil oleogel lotion and preparation method thereof |
-
2021
- 2021-04-25 CN CN202110446867.XA patent/CN113786384A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4443487A (en) * | 1981-02-27 | 1984-04-17 | Lever Brothers Company | Process for producing a spreadable emulsion |
| EP0563593A1 (en) * | 1992-03-02 | 1993-10-06 | Kao Corporation | Oil-in-water type emulsion for whipped cream, powder and whipped cream |
| JPH0799886A (en) * | 1993-09-30 | 1995-04-18 | Snow Brand Milk Prod Co Ltd | Emulsion substitutable for fat, its usage and frozen confectionary using the same |
| US20120034353A1 (en) * | 2010-08-09 | 2012-02-09 | Joerg Eggeling | High Pressure Homogenization In Combination With Functionalized Egg For Production Of Emulsion-Based Food Products |
| CN110215416A (en) * | 2019-06-06 | 2019-09-10 | 浙江大学 | A kind of camellia oil oleogel lotion and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘盼盼等: "不同单硬脂酸甘油酯含量的大豆油油凝胶性能和微观结构分析", 《南京农业大学学报》 * |
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