CN113785822A - Tumor tissue specimen preservation device - Google Patents
Tumor tissue specimen preservation device Download PDFInfo
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- CN113785822A CN113785822A CN202111066286.XA CN202111066286A CN113785822A CN 113785822 A CN113785822 A CN 113785822A CN 202111066286 A CN202111066286 A CN 202111066286A CN 113785822 A CN113785822 A CN 113785822A
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- sealing ring
- extraction
- hole
- cavity
- extraction cavity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
Abstract
The invention provides a tumor tissue specimen preservation device. When the storage device is used, the position of the tissue to be cut is determined and marked on the perspective window; rotating the extraction cavity to enable the specimen protection liquid in the first sealing ring to flow into the liquid storage chamber through the through hole; adjusting a plurality of beams of light of a laser cutting instrument to focus on a cutting point and operating to separate tissues; after cutting, rotating the isolating slide and the extraction cavity to enable the separated tissue part to fall into the extraction groove; finally, the separated tissue is extracted to the outside through a movable door on the side surface of the extraction groove. Through the steps, no external equipment is required to enter the storage device for any operation in the whole process, the tissue specimen can be extracted from the storage device, and the isolation and protection effects of the storage device are effectively improved.
Description
Technical Field
The invention relates to the technical field of medical preservation. In particular to a device for preserving tumor tissue specimens.
Background
The analysis and preservation of tumor tissues can be used as an important basis for the later analysis of relevant pathological diagnosis. The correct treatment of the pathological sample can provide effective guarantee for pathological diagnosis and clinical diagnosis. The most critical step in the treatment of pathological specimens is the fixation and preservation of the pathological specimens. After the pathological sample is separated from the body, the structure of the pathological sample is destroyed because of autolysis and/or putrefaction caused by the change of microenvironment, and simultaneously, cells are degenerated and autolyzed microscopically. The purpose of processing the sample is to maintain the pathological specimen in a pre-isolated state as much as possible by various methods. More importantly, due to the need for microscopic observation, histopathology requires that the ex vivo sample not only be as close to the pre-ex vivo state as possible in macroscopic aspects (such as color, volume, hardness, etc.), but also be as close to the in vivo state as possible in microscopic aspects at the cellular level. Currently, the most common treatment agents for pathological specimens are formaldehyde solutions and ethanol solutions, but both of them have significant drawbacks. The formaldehyde is easy to make the specimen body hard, deformed, faded and not fresh; the formaldehyde solution contains methanol, which is easy to polymerize to generate precipitate and needs to be replaced frequently; methanol has a strong pungent odor, which not only pollutes the environment, but also harms the eyes and respiratory system of the operator to various degrees, seriously harms human health, and is very harmful to first-line medical workers. After the ethanol-containing preservation solution is used for a long time, the body of the sample is also hard, and the fading phenomenon is more obvious than that of formaldehyde; and the samples preserved by the two solutions have the defects of difficult modeling and troublesome transportation.
Referring to the related disclosed technical solutions, solutions such as CN211458669(U), JP2018154617(a), CN208676209(U), etc. make several optimization designs on the structure or storage conditions of the storage device, so that the storage device provides a better or more convenient storage function. However, in the above-mentioned solutions, the specimen to be preserved needs to be contacted with the outside to some extent in the operations such as the replacement of the preservation solution or the cutting of the specimen to be preserved, which is not good for the isolation, protection and preservation of the specimen.
Disclosure of Invention
The invention aims to provide a tumor tissue specimen storage device in order to cut and extract a tumor tissue specimen under the condition of being isolated from the outside to the maximum extent.
The invention adopts the following technical scheme:
a tumor tissue specimen preservation device is characterized by comprising a perspective window, an isolation slide, a first sealing ring, a second sealing ring, a liquid storage chamber and an extraction cavity; the perspective window is embedded in the first end face of the first sealing ring and is used for observing the preserved specimen from the top; the extraction cavity can slide and rotate in the axial direction in the first sealing ring and comprises an extraction groove and a through hole; the isolating slide is placed inside the first sealing ring and covers the first end face of the extraction cavity; the second sealing ring is connected with the liquid storage chamber through threads and is used for receiving the specimen preservation liquid flowing out of the through hole;
when the storage device is used, the position of the tissue to be cut is determined and marked on the perspective window; rotating the extraction cavity to enable the sample protection liquid in the first sealing ring to flow into the liquid storage chamber through the through hole; adjusting a plurality of beams of light of a laser cutting instrument to focus on a cutting point and operating to separate tissues; after cutting, rotating the isolating slide and the extracting cavity to enable the separated tissue part to fall into the extracting groove; finally, the separated tissues are extracted to the outside through a movable door on the side surface of the extraction groove;
the perspective window is made of high-light-transmittance glass, the light transmittance is over 85 percent, and the Mohs hardness is 4 or more;
the isolating glass sheet can be made of transparent glass, ground glass or other optical glass; the isolating slide comprises a circular through hole A and a fan-shaped through hole; sealing materials are added between the circular through hole A and the fan-shaped through hole and the contact surface of the extraction cavity, and are used for sealing the peripheries of the contact surfaces of the two through holes;
the periphery of the circumference of the isolating slide is wrapped with a sealing material, so that the isolating slide divides the inside of the first sealing ring into two closed spaces;
the extraction cavity comprises a through hole B; the circle center position of the through hole B is consistent with that of the through hole A, and the two through holes are communicated after reaching a proper angle by rotating one of the extraction cavity or the partition glass; the extraction cavity comprises a fan-shaped extraction groove; the opening of the extraction groove is positioned on the first end surface of the extraction cavity, but does not penetrate to the other end surface of the extraction cavity; the first end surface of the extraction cavity is closely mounted with the isolating slide;
the extraction groove is provided with the movable door close to the outer side of the periphery of the extraction cavity; the movable door can be turned over from the outside, and a tool is used for taking out the sample tissue in the extraction groove;
the inner diameter of the second sealing ring is larger than the outer diameter of the extracting cavity; the extraction cavity is embedded in the second sealing ring when being installed; the inner surface of the second sealing ring and the outer surface of the extraction cavity are sealed through a sealing material;
the inner surface of the second seal ring comprises a threaded segment A; the outer surface of the extraction cavity comprises a section of threaded section B; through screwing the thread section A and the thread section B, and through rotating the second sealing ring, the second sealing ring and the extraction cavity generate relative displacement in the axial direction;
the inside of the liquid storage chamber is a circular cavity; the diameter of the circular cavity is larger than the outer diameter of the second sealing ring; the inner surface of the reservoir comprises a threaded segment C; the outer surface of the second seal ring comprises a threaded segment D; through the screwing of the thread section C and the thread section D, and through rotating the liquid storage chamber, the liquid storage chamber and the second sealing ring generate relative displacement in the axial direction.
The beneficial effects obtained by the invention are as follows:
1. the invention adopts the laser internal focusing cutting technology to cut the sample tissue, can finish the tissue separation operation under the condition of not contacting the tissue, and has complete and undamaged cut;
2. according to the technical scheme adopted by the invention, in the whole extraction link, the tissue preservation is always carried out in the internal environment of the protection device, and the specimen is not polluted by the external environment;
3. the specific size of the preservation device can be designed according to the size of the specimen, and the operation is simple, so that the preservation device is beneficial to popularization and use in practical application;
4. the invention adopts a plurality of sealing mechanisms to seal and isolate the preservation cavity, and the selected materials can be kept stable at low temperature, thereby being beneficial to the long-term preservation of tumor tissue specimens.
Drawings
The invention will be further understood from the following description in conjunction with the accompanying drawings. The components in the figures are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the embodiments. Like reference numerals designate corresponding parts throughout the different views.
FIG. 1 is a schematic composition of the present invention;
FIG. 2 is a schematic view of various details of the present invention;
FIG. 3 is a schematic diagram of an inner focus cutting using multiple lasers;
FIG. 4 is a schematic view of a medical laser cutting instrument;
fig. 5 is a schematic view of a tumor tissue specimen immersed in a specimen preservation solution.
The reference numbers illustrate: 100-a preservation device; 101-a see-through window; 102-a magnetic ring; 103-a first sealing ring; 104-an extraction chamber; 105-a spacer slide; 106-sealing plug; 107-second sealing ring; 108-a reservoir; 201-a sealing material; 202-via a; 203-via B; 204-fan-shaped through holes; 205-an extraction tank; 206-thread segment a; 207-thread segment C; 208-thread segment D; 209-thread segment B.
Detailed Description
In order to make the technical solution and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the embodiments thereof; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Other systems, methods, and/or features of the present embodiments will become apparent to those skilled in the art upon review of the following detailed description. It is intended that all such additional systems, methods, features and advantages be included within this description, be within the scope of the invention, and be protected by the accompanying claims. Additional features of the disclosed embodiments are described in, and will be apparent from, the detailed description that follows.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it is to be understood that if there is an orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", etc. based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not intended to indicate or imply that the device or assembly referred to must have a specific orientation.
The first embodiment is as follows:
a tumor tissue specimen preservation device comprises a perspective window, an isolation slide, a first sealing ring, a second sealing ring, a liquid storage chamber and an extraction cavity; the perspective window is embedded in the first end face of the first sealing ring and is used for observing the preserved specimen from the top; the extraction cavity can slide and rotate in the axial direction in the first sealing ring and comprises an extraction groove and a through hole; the isolating slide is placed inside the first sealing ring and covers the first end face of the extraction cavity; the second sealing ring is connected with the liquid storage chamber through threads and is used for receiving the specimen preservation liquid flowing out of the through hole;
when the storage device is used, the position of the tissue to be cut is determined and marked on the perspective window; rotating the extraction cavity to enable the sample protection liquid in the first sealing ring to flow into the liquid storage chamber through the through hole; adjusting a plurality of beams of light of a laser cutting instrument to focus on a cutting point and operating to separate tissues; after cutting, rotating the isolating slide and the extracting cavity to enable the separated tissue part to fall into the extracting groove; finally, the separated tissues are extracted to the outside through a movable door on the side surface of the extraction groove;
the perspective window is made of high-light-transmittance glass, the light transmittance is over 85 percent, and the Mohs hardness is 4 or more;
the isolating glass sheet can be made of transparent glass, ground glass or other optical glass; the isolating slide comprises a circular through hole A and a fan-shaped through hole; sealing materials are added between the circular through hole A and the fan-shaped through hole and the contact surface of the extraction cavity, and are used for sealing the peripheries of the contact surfaces of the two through holes;
the periphery of the circumference of the isolating slide is wrapped with a sealing material, so that the isolating slide divides the inside of the first sealing ring into two closed spaces;
the extraction cavity comprises a through hole B; the circle center position of the through hole B is consistent with that of the through hole A, and the two through holes are communicated after reaching a proper angle by rotating one of the extraction cavity or the partition glass; the extraction cavity comprises a fan-shaped extraction groove; the opening of the extraction groove is positioned on the first end surface of the extraction cavity, but does not penetrate to the other end surface of the extraction cavity; the first end surface of the extraction cavity is closely mounted with the isolating slide;
the extraction groove is provided with the movable door close to the outer side of the periphery of the extraction cavity; the movable door can be turned over from the outside, and a tool is used for taking out the sample tissue in the extraction groove;
the inner diameter of the second sealing ring is larger than the outer diameter of the extracting cavity; the extraction cavity is embedded in the second sealing ring when being installed; the inner surface of the second sealing ring and the outer surface of the extraction cavity are sealed through a sealing material;
the inner surface of the second seal ring comprises a threaded segment A; the outer surface of the extraction cavity comprises a section of threaded section B; through screwing the thread section A and the thread section B, and through rotating the second sealing ring, the second sealing ring and the extraction cavity generate relative displacement in the axial direction;
the inside of the liquid storage chamber is a circular cavity; the diameter of the circular cavity is larger than the outer diameter of the second sealing ring; the inner surface of the reservoir comprises a threaded segment C; the outer surface of the second seal ring comprises a threaded segment D; the thread section C is screwed with the thread section D, and the liquid storage chamber is rotated to enable the liquid storage chamber and the second sealing ring to generate relative displacement in the axial direction;
when the storage operation is carried out, the method comprises the following operation steps;
s1, placing the extraction cavity into the first sealing ring;
s2, placing the isolating slide into the first sealing ring and covering the first end face of the extraction cavity;
s3, placing the tumor tissue specimen to be preserved on the isolating glass sheet;
s4, rotating the extraction cavity to enable the through hole and the extraction groove of the extraction cavity to be staggered with the circular through hole and the fan-shaped through hole on the isolation slide;
s5, injecting a specimen preservation solution and ensuring that the whole specimen is immersed;
s6, covering the perspective window on the spigot at the first end of the first sealing ring;
s7, screwing the threaded section A of the second sealing ring into the threaded section B of the extraction cavity;
s8, screwing the thread section C of the liquid storage chamber into the thread section D of the second sealing ring;
after the specimen preservation liquid in the first sealing ring is ensured not to leak, the four sections of thread sections are screwed tightly, so that the appearance of the preservation device reaches the most compact state;
when the specimen tissue needs to be separated, in order to ensure that a plurality of cutting light rays of the laser cutting instrument can ensure enough power when passing through the perspective window but do not damage the surface of the perspective window, the related technicians need to calculate the power of each cutting light ray; because the laser power for cutting the tumor tissue is necessarily far less than the power required for cutting the glass material, even if the single-beam cutting power q is far less than the minimum power for melting the glass material of the perspective window, the tumor tissue behind the perspective window can be necessarily cut by superposing a plurality of beams of light and focusing the plurality of beams of light on one point at the same time;
furthermore, in order to enable the laser to be accurately focused on one point after the laser passes through the perspective window for one time of refraction, the light transmittance and the purity of the perspective window and the flatness and the parallelism of two end surfaces of the perspective window are controlled within a standard range; preferably, the light transmittance of the selected glass material is more than 85%, the purity is less than 2%, and the flatness tolerance and the parallelism tolerance are both more than 6 grades;
furthermore, before cutting operation, the position of the tissue to be cut needs to be determined and marked on the perspective window; in order to avoid the influence of the specimen protection liquid on the differential refraction of the laser, the specimen protection liquid in the first sealing ring needs to be emptied; therefore, the extraction cavity needs to be rotated to enable the through hole A to be communicated with the through hole B, so that the specimen protection liquid in the first sealing cavity flows into the liquid storage chamber through the through hole; adjusting a plurality of beams of light of a laser cutting instrument to focus on a cutting point and operating to separate tissues; after cutting, rotating the isolating slide and the extracting cavity to enable the separated tissue part to fall into the extracting groove through the fan-shaped through hole; and finally, extracting the separated tissues to the outside through a movable door on the side surface of the extraction groove.
Example two:
this embodiment should be understood to include at least all of the features of any of the foregoing embodiments and further modifications thereon; a tumor tissue specimen preservation device comprises a perspective window, an isolation slide, a first sealing ring, a second sealing ring, a liquid storage chamber and an extraction cavity; the perspective window is embedded in the first end face of the first sealing ring and is used for observing the preserved specimen from the top; the extraction cavity can slide and rotate in the axial direction in the first sealing ring and comprises an extraction groove and a through hole; the isolating slide is placed inside the first sealing ring and covers the first end face of the extraction cavity; the second sealing ring is connected with the liquid storage chamber through threads and is used for receiving the specimen preservation liquid flowing out of the through hole;
when the storage device is used, the position of the tissue to be cut is determined and marked on the perspective window; rotating the extraction cavity to enable the sample protection liquid in the first sealing ring to flow into the liquid storage chamber through the through hole; adjusting a plurality of beams of light of a laser cutting instrument to focus on a cutting point and operating to separate tissues; after cutting, rotating the isolating slide and the extracting cavity to enable the separated tissue part to fall into the extracting groove; finally, the separated tissues are extracted to the outside through a movable door on the side surface of the extraction groove;
the perspective window is made of high-light-transmittance glass, the light transmittance is over 85 percent, and the Mohs hardness is 4 or more;
the isolating glass sheet can be made of transparent glass, ground glass or other optical glass; the isolating slide comprises a circular through hole A and a fan-shaped through hole; sealing materials are added between the circular through hole A and the fan-shaped through hole and the contact surface of the extraction cavity, and are used for sealing the peripheries of the contact surfaces of the two through holes;
the periphery of the circumference of the isolating slide is wrapped with a sealing material, so that the isolating slide divides the inside of the first sealing ring into two closed spaces;
the extraction cavity comprises a through hole B; the circle center position of the through hole B is consistent with that of the through hole A, and the two through holes are communicated after reaching a proper angle by rotating one of the extraction cavity or the partition glass; the extraction cavity comprises a fan-shaped extraction groove; the opening of the extraction groove is positioned on the first end surface of the extraction cavity, but does not penetrate to the other end surface of the extraction cavity; the first end surface of the extraction cavity is closely mounted with the isolating slide;
the extraction groove is provided with the movable door close to the outer side of the periphery of the extraction cavity; the movable door can be turned over from the outside, and a tool is used for taking out the sample tissue in the extraction groove;
the inner diameter of the second sealing ring is larger than the outer diameter of the extracting cavity; the extraction cavity is embedded in the second sealing ring when being installed; the inner surface of the second sealing ring and the outer surface of the extraction cavity are sealed through a sealing material;
the inner surface of the second seal ring comprises a threaded segment A; the outer surface of the extraction cavity comprises a section of threaded section B; through screwing the thread section A and the thread section B, and through rotating the second sealing ring, the second sealing ring and the extraction cavity generate relative displacement in the axial direction;
the inside of the liquid storage chamber is a circular cavity; the diameter of the circular cavity is larger than the outer diameter of the second sealing ring; the inner surface of the reservoir comprises a threaded segment C; the outer surface of the second seal ring comprises a threaded segment D; the thread section C is screwed with the thread section D, and the liquid storage chamber is rotated to enable the liquid storage chamber and the second sealing ring to generate relative displacement in the axial direction;
after the cutting is finished, the isolating slide needs to be adjusted, so that the through hole A and the fan-shaped through hole of the isolating slide can be aligned to a target position, and then the next operation is carried out; in order to make the rotation of the isolating slide more convenient and flexible, the isolating slide is further improved by the embodiment; a plurality of magnet devices are arranged on one side of the outer edge of the isolating slide; the magnetic field directions of the plurality of magnet devices are along the radial direction; the magnet device is wrapped by a sealing material and distributed in the sealing material at the outer edge of the isolating slide;
further, a magnetic ring is arranged outside the first sealing ring; the magnetic ring may be composed of a plurality of distributed magnets, preferably a set of switching electromagnetic devices; the direction of a differential magnetic field of the magnetic force ring is the same as that of the magnetic fields of the magnets of the isolating slide, so that the isolating slide is relatively fixed after being acted by the magnetic force ring through magnetic force and can be further driven to rotate by the magnetic force ring;
in the implementation operation, a magnetic ring is sleeved in the first sealing ring and moves to the position of the isolating slide along the axial direction; starting an electromagnetic switch of the magnetic ring, adjusting the angle of the magnetic ring, and observing whether the isolating slide is sucked by the magnetic ring and can be driven from the perspective window; rotating the magnetic ring to make the isolating slide rotate along with the rotation of the magnetic ring and make the through hole A reach the position of the through hole B; after the two through holes are communicated, waiting for the discharge of the specimen protection liquid; further, the magnetic ring is moved to drive the isolating slide to rotate, and the fan-shaped through hole is arranged below the position to be cut; further, a later cutting operation may be performed.
Example three:
this embodiment should be understood to include at least all of the features of any of the embodiments described above and further refinements thereto: a tumor tissue specimen preservation device comprises a perspective window, an isolation slide, a first sealing ring, a second sealing ring, a liquid storage chamber and an extraction cavity; the perspective window is embedded in the first end face of the first sealing ring and is used for observing the preserved specimen from the top; the extraction cavity can slide and rotate in the axial direction in the first sealing ring and comprises an extraction groove and a through hole; the isolating slide is placed inside the first sealing ring and covers the first end face of the extraction cavity; the second sealing ring is connected with the liquid storage chamber through threads and is used for receiving the specimen preservation liquid flowing out of the through hole;
when the storage device is used, the position of the tissue to be cut is determined and marked on the perspective window; rotating the extraction cavity to enable the sample protection liquid in the first sealing ring to flow into the liquid storage chamber through the through hole; adjusting a plurality of beams of light of a laser cutting instrument to focus on a cutting point and operating to separate tissues; after cutting, rotating the isolating slide and the extracting cavity to enable the separated tissue part to fall into the extracting groove; finally, the separated tissues are extracted to the outside through a movable door on the side surface of the extraction groove;
the perspective window is made of high-light-transmittance glass, the light transmittance is over 85 percent, and the Mohs hardness is 4 or more;
the isolating glass sheet can be made of transparent glass, ground glass or other optical glass; the isolating slide comprises a circular through hole A and a fan-shaped through hole; sealing materials are added between the circular through hole A and the fan-shaped through hole and the contact surface of the extraction cavity, and are used for sealing the peripheries of the contact surfaces of the two through holes;
the periphery of the circumference of the isolating slide is wrapped with a sealing material, so that the isolating slide divides the inside of the first sealing ring into two closed spaces;
the extraction cavity comprises a through hole B; the circle center position of the through hole B is consistent with that of the through hole A, and the two through holes are communicated after reaching a proper angle by rotating one of the extraction cavity or the partition glass; the extraction cavity comprises a fan-shaped extraction groove; the opening of the extraction groove is positioned on the first end surface of the extraction cavity, but does not penetrate to the other end surface of the extraction cavity; the first end surface of the extraction cavity is closely mounted with the isolating slide;
the extraction groove is provided with the movable door close to the outer side of the periphery of the extraction cavity; the movable door can be turned over from the outside, and a tool is used for taking out the sample tissue in the extraction groove;
the inner diameter of the second sealing ring is larger than the outer diameter of the extracting cavity; the extraction cavity is embedded in the second sealing ring when being installed; the inner surface of the second sealing ring and the outer surface of the extraction cavity are sealed through a sealing material;
the inner surface of the second seal ring comprises a threaded segment A; the outer surface of the extraction cavity comprises a section of threaded section B; through screwing the thread section A and the thread section B, and through rotating the second sealing ring, the second sealing ring and the extraction cavity generate relative displacement in the axial direction;
the inside of the liquid storage chamber is a circular cavity; the diameter of the circular cavity is larger than the outer diameter of the second sealing ring; the inner surface of the reservoir comprises a threaded segment C; the outer surface of the second seal ring comprises a threaded segment D; the thread section C is screwed with the thread section D, and the liquid storage chamber is rotated to enable the liquid storage chamber and the second sealing ring to generate relative displacement in the axial direction;
in order to further facilitate the replacement of the specimen protection solution in the preservation device of the preservation box under the condition of isolating external contact, the embodiment is further optimized; a threaded hole E is formed in one side of the through hole B of the extraction cavity; the central axis of the threaded hole E points to the central axis of the extraction cavity, and the central axis of the threaded hole E intersects and is perpendicular to the central axis of the through hole B; furthermore, a sealing plug with a thread is arranged in the threaded hole E; the thread of the sealing plug can be tightly matched with the threaded hole E; the whole length of the sealing plug needs to be calculated, so that the sealing plug can be screwed into the threaded hole E to completely block the through hole B and does not protrude out of the extraction cavity; one end of the sealing plug close to the outer side is provided with a linear or cross-shaped groove, so that the sealing plug can be screwed by a screwdriver;
further, an adjustable micro valve is added at the bottom of the liquid storage chamber; the micro valve is operated by adopting a rotary switch mode to open or close the valve; when the operation of emptying the specimen protection solution is carried out, the method comprises the following steps:
s1, rotating the liquid storage chamber to expand the space surrounded by the liquid storage chamber and the second sealing ring so as to accommodate more specimen protection liquid;
s2, operating the magnetic ring to align the through hole A of the isolating slide with the through hole B of the extraction cavity;
s3, rotating the second sealing ring to expose the sealing plug on the side surface of the extraction cavity in the visual field; the storage device is erected, the sealing plug is screwed by a screwdriver, so that two ends of the through hole B are communicated, and the specimen protection liquid immediately flows into the liquid storage chamber;
s4, using a negative pressure suction device such as a liquid pump to connect the micro valve on the liquid storage chamber, adjusting the switch of the adjusting valve, opening the suction device, and evacuating the specimen protection liquid in the protection device by using negative pressure;
in the above-described evacuation step, the specimen-protecting liquid in the storage device is evacuated as much as possible, and then the operation of cutting the tissue or the operation of periodically replacing the specimen-protecting liquid is performed, so that the stored material in the storage device is isolated from the outside to the maximum extent.
In the above embodiments, the descriptions of the respective embodiments have respective emphasis, and reference may be made to the related descriptions of other embodiments for parts that are not described or illustrated in a certain embodiment.
Although the invention has been described above with reference to various embodiments, it should be understood that many changes and modifications may be made without departing from the scope of the invention. That is, the methods, systems, and devices discussed above are examples. Various configurations may omit, substitute, or add various procedures or components as appropriate. For example, in alternative configurations, the methods may be performed in an order different than that described, and/or various components may be added, omitted, and/or combined. Moreover, features described with respect to certain configurations may be combined in various other configurations, as different aspects and elements of the configurations may be combined in a similar manner. Further, elements therein may be updated as technology evolves, i.e., many elements are examples and do not limit the scope of the disclosure or claims.
Specific details are given in the description to provide a thorough understanding of the exemplary configurations including implementations. However, configurations may be practiced without these specific details, for example, well-known circuits, processes, algorithms, structures, and techniques have been shown without unnecessary detail in order to avoid obscuring the configurations. This description provides example configurations only, and does not limit the scope, applicability, or configuration of the claims. Rather, the foregoing description of the configurations will provide those skilled in the art with an enabling description for implementing the described techniques. Various changes may be made in the function and arrangement of elements without departing from the spirit or scope of the disclosure.
In conclusion, it is intended that the foregoing detailed description be regarded as illustrative rather than limiting, and that it be understood that these examples are illustrative only and are not intended to limit the scope of the invention. After reading the description of the invention, the skilled person can make various changes or modifications to the invention, and these equivalent changes and modifications also fall into the scope of the invention defined by the claims.
Claims (9)
1. A tumor tissue specimen preservation device is characterized by comprising a perspective window, an isolation slide, a first sealing ring, a second sealing ring, a liquid storage chamber and an extraction cavity; the perspective window is embedded in the first end face of the first sealing ring and is used for observing the preserved specimen from the top; the extraction cavity can slide and rotate in the axial direction in the first sealing ring and comprises an extraction groove and a through hole; the isolating slide is placed inside the first sealing ring and covers the first end face of the extraction cavity; the second sealing ring is connected with the liquid storage chamber through threads and is used for receiving the specimen preservation liquid flowing out of the through hole;
when the storage device is used, the position of the tissue to be cut is determined and marked on the perspective window; rotating the extraction cavity to enable the sample protection liquid in the first sealing ring to flow into the liquid storage chamber through the through hole; adjusting a plurality of beams of light of a laser cutting instrument to focus on a cutting point and operating to separate tissues; after cutting, rotating the isolating slide and the extracting cavity to enable the separated tissue part to fall into the extracting groove; and finally, extracting the separated tissues to the outside through a movable door on the side surface of the extraction groove.
2. The device for preserving a tumor tissue specimen according to claim 1, wherein the transparent window is made of high-transmittance glass, the transmittance is more than 85%, and the mohs hardness is 4 or more.
3. The device for preserving a tumor tissue specimen according to claim 2, wherein the material of the isolating slide can be transparent glass, ground glass or other optical glass; the isolating slide comprises a circular through hole A and a fan-shaped through hole; and sealing materials are added between the circular through hole A and the fan-shaped through hole and the contact surface of the extraction cavity and are used for sealing the peripheries of the contact surfaces of the two through holes.
4. The device for preserving a tumor tissue specimen as claimed in claim 3, wherein the isolating slide is wrapped with a sealing material at its circumference so that the isolating slide separates the inside of the first sealing ring into two closed spaces.
5. The device for preserving a tumor tissue specimen according to claim 4, wherein the extraction chamber comprises a through hole B; the circle center position of the through hole B is consistent with that of the through hole A, and the two through holes are communicated after reaching a proper angle by rotating one of the extraction cavity or the partition glass; the extraction cavity comprises a fan-shaped extraction groove; the opening of the extraction groove is positioned on the first end surface of the extraction cavity, but does not penetrate to the other end surface of the extraction cavity; the first end face of the extraction chamber is mounted in close proximity to the spacer slide.
6. The apparatus for preserving tumor tissue specimen as claimed in claim 5, wherein the extraction trough is provided with the movable door near the outer side of the extraction cavity circumference; the movable door can be turned over from the outside, and a tool is used to take out the specimen tissue inside the extraction tank.
7. The device for preserving a tumor tissue specimen according to claim 6, wherein the inner diameter of the second sealing ring is larger than the outer diameter of the extraction cavity; the extraction cavity is embedded in the second sealing ring when being installed; the inner surface of the second sealing ring and the outer surface of the extraction cavity are sealed through a sealing material.
8. The device for preserving a tumor tissue specimen according to claim 7, wherein the inner surface of the second sealing ring comprises a segment of a threaded segment A; the outer surface of the extraction cavity comprises a section of threaded section B; and through screwing the thread section A and the thread section B, and rotating the second sealing ring, the second sealing ring and the extraction cavity generate relative displacement in the axial direction.
9. The device for preserving a tumor tissue specimen according to claim 8, wherein the interior of the reservoir is a circular cavity; the diameter of the circular cavity is larger than the outer diameter of the second sealing ring; the inner surface of the reservoir comprises a threaded segment C; the outer surface of the second seal ring comprises a threaded segment D; through the screwing of the thread section C and the thread section D, and through rotating the liquid storage chamber, the liquid storage chamber and the second sealing ring generate relative displacement in the axial direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111066286.XA CN113785822A (en) | 2021-09-13 | 2021-09-13 | Tumor tissue specimen preservation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111066286.XA CN113785822A (en) | 2021-09-13 | 2021-09-13 | Tumor tissue specimen preservation device |
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| Publication Number | Publication Date |
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| CN113785822A true CN113785822A (en) | 2021-12-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111066286.XA Withdrawn CN113785822A (en) | 2021-09-13 | 2021-09-13 | Tumor tissue specimen preservation device |
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| CN (1) | CN113785822A (en) |
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| CN116267901A (en) * | 2023-05-19 | 2023-06-23 | 山东第一医科大学附属省立医院(山东省立医院) | Human body local tumor preservation device |
| CN115590012B (en) * | 2022-09-27 | 2024-05-10 | 南京海合源农业科技服务有限公司 | Device and method for softening insect specimen preparation |
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Application publication date: 20211214 |