CN113762435A - RFID-based biological sample library intelligent management system and management method thereof - Google Patents

RFID-based biological sample library intelligent management system and management method thereof Download PDF

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CN113762435A
CN113762435A CN202011245826.6A CN202011245826A CN113762435A CN 113762435 A CN113762435 A CN 113762435A CN 202011245826 A CN202011245826 A CN 202011245826A CN 113762435 A CN113762435 A CN 113762435A
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sample
rfid
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biological sample
antenna
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李谡
戴欢
石凌云
俞雯超
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Hangzhou Technology Co Ltd
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    • G06COMPUTING; CALCULATING OR COUNTING
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    • G06K17/00Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations
    • G06K17/0022Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisions for transferring data to distant stations, e.g. from a sensing device
    • G06K17/0029Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisions for transferring data to distant stations, e.g. from a sensing device the arrangement being specially adapted for wireless interrogation of grouped or bundled articles tagged with wireless record carriers
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    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00Methods specially adapted for identifying library members
    • C40B20/04Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00ICT programming tools or database systems specially adapted for bioinformatics

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Abstract

The invention discloses an RFID-based intelligent management system and a management method of a biological sample library, and the system comprises a cloud server, terminal equipment, a portable sample box scanner and a sample checking instrument, wherein the portable sample box scanner is used for scanning a sample box, and the sample checking instrument is used for checking sample test tubes in the sample box; the sample checking instrument comprises a central processing unit, an antenna control module, a radio frequency identification module, a radio frequency unit and a response unit, wherein the radio frequency unit comprises a plurality of Radio Frequency Identification (RFID) antennas, the response unit comprises a plurality of RFID electronic tags, the RFID electronic tags are attached to the sample box, each radio frequency antenna corresponds to one of the RFID electronic tags of the response unit, the radio frequency antennas are matched with the radio frequency identification unit, and the central processing unit and the cloud server compare different RFID electronic tags. The invention can effectively carry out digital management and improve the identification and tracking of the biological sample library.

Description

RFID-based biological sample library intelligent management system and management method thereof
Technical Field
The invention belongs to the field of biological sample management, and particularly relates to an intelligent management system and method for a biological sample library based on RFID.
Background
The biological sample bank is also called biological bank (Biobank) and mainly refers to standardized collection, processing, storage and application of samples such as biological macromolecules, cells, tissues and organs of healthy and disease organisms (including human organ tissues, whole blood, blood plasma, blood serum, biological body fluid or processed biological samples (DNA, RNA, protein and the like), and clinical, pathological, treatment, follow-up visit, informed consent and other data related to the biological samples, and a quality control, information management and application system thereof.
At present, most hospitals or scientific research units adopt manual methods to manage biological sample libraries. There are problems as follows:
the space utilization rate of the preservation box is as follows: after a certain amount of samples are accumulated, manual searching and storing of the samples are difficult and error-prone, and the utilization efficiency of containers (such as refrigerators and liquid nitrogen tanks) for storing the samples is gradually reduced. Because the samples may be stored in a sorted order initially, but depending on the scientific needs, the samples are taken from different positions of the container, and a large amount of scattered vacant space is generated in the container after a period of time, and the scattered vacant space cannot be effectively utilized. It is necessary to automatically assign and search the sample storage positions by using an information system, thereby improving the space utilization efficiency of containers such as refrigerators.
Accuracy of sampling: this is directly related to experimental results and research conclusions and is sometimes overlooked. We have once heard of such cases: a subject for studying a female disease provides a commercial service with a collection of patient samples, some of which are found to be male during the course of the study, which results in a substantial loss of value. To avoid such problems, the barcode can be scanned by a mature low-temperature barcode labeling technology to check related information in a software tool, so that the sampling accuracy can be effectively ensured.
Managing matched clinical data: the purpose of collecting and storing samples is to screen the samples of patients according to the needs of clinical scientific research, obtain the correlation between the external symptoms and the internal expressions of genes, proteins and the like of the patients through molecular biology experiments, if one sample lacks complete matched clinical data, the screening basis is insufficient, and the scientific research value of the sample is greatly reduced.
Quality control of biological samples: the method comprises the steps of sample collection and subpackaging, warehousing, sampling and ex-warehouse monitoring of the quality of the samples in the whole process, and avoids the quality problems of pollution, degradation and the like of the samples.
Therefore, how to improve the safety and reliability of the experiment of each biological sample library and realize the automatic recording of data so as to facilitate the inquiry, analysis and statistics in the future is a problem to be solved in the prior art.
In the informatization process of biological sample library management, a scheme of adopting a two-dimensional code identification technology appears.
The use of two-dimensional code management can standardize the management of biological sample libraries. By scanning the two-dimensional code on the side surface or the bottom of the sample tube and inquiring the corresponding database in a matching way, the information of the sample can be obtained quickly.
Meanwhile, compared with the traditional manual management mode, the two-dimensional code has the advantages of simplicity and convenience in management, correct sample information and the like, and meets the requirement for informatization management of a biological sample library. Meanwhile, the two-dimension code label is low in cost, and the upgrading difficulty is small for a sample library needing to be deployed with a two-dimension code identification scheme.
The two-dimensional code recognition technology has inevitable problems:
1. the two-dimension code has no uniform coding protocol in the international range, and the matched database has high development difficulty
2. In an actual use scenario, since the biological sample is usually stored in a low-temperature or ultra-low-temperature environment, after the biological sample tube with the two-dimensional code label is taken out, frost fog is easily formed on the surface, and normal reading of the two-dimensional code reader is affected. If a defrosting machine is used or frost fog is waited to dissipate, the sample needs to be placed in a non-low temperature environment for a long time, which is easy to cause deterioration of the biological sample
3. The two-dimensional code recognizer recognizes sample information by adopting visible light such as laser, the system structure is complex, and multiple processes such as focusing are often needed to read multiple two-dimensional codes. Due to the complexity of the structure, it is also difficult to apply the two-dimensional code recognizer to the field of automation management.
Disclosure of Invention
In order to solve the defects and shortcomings in the prior art, the invention provides a method which can effectively carry out digital management, perfects the identification and tracking of a biological sample library, distributes unique electronic numbers for different biological samples, checks the correctness of the biological samples by a computer according to the uniqueness in the processing of each link, automatically alarms by voice and images in any error system, strictly controls the experimental process and avoids missing the operation links; the error caused by the human factors in the laboratory is avoided; the RFID-based biological sample library intelligent management system and the management method thereof provide data for the management of a laboratory to support the automatic recording, permanent storage, traceability and retrieval of all operation data and can automatically analyze reports.
The technical scheme of the invention is as follows: an RFID-based intelligent management system for a biological sample library comprises a cloud server, a terminal device connected to the cloud server, a portable sample box scanner and a sample checking instrument, wherein the portable sample box scanner is used for scanning a sample box, and the sample checking instrument is used for checking sample test tubes in the sample box; the sample checking instrument comprises a central processing unit, an antenna control module and a radio frequency identification module which are connected to the central processing unit, a radio frequency unit which is connected to the antenna control module and the radio frequency identification module, and a response unit which is connected to the radio frequency unit, wherein the radio frequency unit comprises a plurality of wireless radio frequency antennas, the response unit comprises a plurality of RFID electronic tags, the RFID electronic tags are attached to a sample box, each wireless radio frequency antenna corresponds to one of the RFID electronic tags of the response unit, the wireless radio frequency antennas are matched with the radio frequency identification unit, and the central processing unit and a cloud server compare different RFID electronic tags.
Preferably, the central processing unit adopts an embedded microprocessor, controls the antenna control module and the radio frequency identification module downwards, communicates with the cloud server, receives a cloud server instruction, performs front-end processing on received data, and then sends the data to the cloud server.
Preferably, the antenna control module is a general radio frequency switch, and the radio frequency switch receives and decodes a control signal of the central processing unit to control the on/off of any radio frequency unit at any time.
Preferably, the radio frequency identification module is an RFID reader, and is designed to perform multi-antenna reading and writing according to a position and single-antenna reading and writing at an appointed position, and performs polling operation in cooperation with the antenna control module.
Preferably, the radio frequency unit is designed into a matrix type, namely a matrix RFID antenna, and is controlled by the antenna control module, the antennas at different positions have no crosstalk, so that the RFID tag can be accurately positioned, and the radio frequency unit adopts a UHF frequency band antenna and is designed into a near field antenna, so that the interference among the antennas is reduced.
Preferably, the RFID electronic tag of the answering unit is the identification characteristic of the biological sample, adopts EPC-C1-G2 global uniform coding and has a global unique identification code.
A management method of the RFID-based intelligent management system for biological sample libraries of claim 1, binding the sample boxes and sample tubes in the biological sample library with RFID electronic tags; the user communicates with the cloud server through the terminal equipment; a portable sample box scanner and a sample checking instrument which are manufactured based on the RFID technology are connected with a cloud server; the user controls the RFID reader-writer to work in the terminal equipment, the antenna control module controls the on-off of the wireless radio frequency antenna according to the position by designing the matrix RFID antenna, the radio frequency identification module sends a reading instruction to read the RFID electronic tag attached to the biological sample, and the central processing unit collects and obtains biological sample data with position information so as to realize the functions of checking and positioning the biological sample through the RFID technology.
Preferably, the management method of the RFID-based biological sample library intelligent management system comprises the following specific steps:
1) a user logs in the terminal equipment, and the cloud server records an operator according to login information;
2) recording:
a) a user uses the terminal equipment to control the sample checking instrument to enter a recording working mode;
b) placing a sample to be recorded at a recording position;
c) the characteristic code of the sample to be input is collected by a sample checking instrument and reflected on the terminal equipment through a cloud processor;
d) the user records the sample information to be input in the database corresponding to the feature code;
e) placing a sample box into which the sample needs to be placed in a reading area by a user;
f) reading the characteristic code of the sample box and recording the position of the sample placed in the sample box;
g) the recording process is finished;
3) checking:
a) a user controls the sample checking instrument to enter a checking working mode by using the terminal equipment;
b) placing the sample cartridge entirely in the sample inventorying instrument;
c) firstly, reading a sample box feature code and confirming sample box information;
d) entering a polling working mode, and reading the feature codes of the samples in the sample box according to the positions;
e) after reading, matching the read feature codes with original data of the database, and reflecting the feature codes to a display interface of the equipment terminal;
f) judging an abnormal condition and feeding back to a user;
g) finishing the checking process;
4) searching:
a) a user inputs a search condition in terminal equipment;
b) the system gives the position information of the sample according to the database;
c) according to the information, the user firstly uses the portable sample box scanner to scan the sample box where the sample is located;
d) placing the corresponding sample box into a sample counting instrument reading area, and reading the specified position to confirm whether the sample at the position is the required sample;
e) the search process ends.
Preferably, when the user searches, uses, puts back and shifts the biological sample, the user should perform the inventory before warehousing again and record the operation in the system to ensure the correctness of the biological sample data.
Preferably, the RFID electronic tag has the capability of normally working after resisting high-dose gamma radiation, and can maintain the function after the biological sample tube is sterilized through medical use.
The invention has the following beneficial effects:
1) the digital management can be effectively carried out, and the identification and tracking samples of the biological sample library are perfected: a) the extracted and subpackaged samples can be traced back to the original samples through an information system and are associated with the information of the original samples; b) each sample should have and only has a unique identification symbol in an information system; c) the whole process of collecting, processing, storing, delivering, transporting, returning to store again after use and the like of the sample is effectively recorded; d) the transfer of the biological samples should be recorded in time, and the information system can trace back the change of the storage position of each sample;
2) the problem of safety worry in the process of collecting and storing the biological sample library is solved, unique electronic numbers are distributed for different biological samples, the correctness of the biological samples is checked by a computer according to the uniqueness in the processing of each link, any error system can automatically give an alarm through voice and images, the system can strictly control the experimental process, and operation links are prevented from being missed;
3) the system avoids errors caused by human factors in the laboratory, provides data for the management of the laboratory to support all automatic operation data recording, permanently stores, can be traced, can be retrieved, can automatically analyze reports, and has positive promoting significance for the process improvement of the laboratory.
Drawings
FIG. 1 is a system wiring diagram of the present invention;
FIG. 2 is a block diagram of the system of the present invention;
FIG. 3 is a schematic diagram of an array antenna circuit of the RFID reader of the present invention;
FIG. 4 is an enlarged schematic view at I of FIG. 3;
FIG. 5 is an enlarged schematic view at II of FIG. 3;
FIG. 6 is an enlarged schematic view at III of FIG. 3;
FIG. 7 is an enlarged view of the point IV in FIG. 3;
fig. 8 is an enlarged view of v in fig. 3.
Detailed Description
The present invention will be described in further detail with reference to the attached drawings, but the present invention is not limited thereto.
As shown in fig. 1 to 8, an RFID-based intelligent management system for a biological sample library includes a cloud server, a terminal device connected to the cloud server, a portable sample box scanner for scanning a sample box, and a sample inventory instrument for inventorying sample test tubes in the sample box; the sample checking instrument comprises a central processing unit, an antenna control module and a radio frequency identification module which are connected to the central processing unit, a radio frequency unit which is connected to the antenna control module and the radio frequency identification module, and a response unit which is connected to the radio frequency unit, wherein the radio frequency unit comprises a plurality of wireless radio frequency antennas, the response unit comprises a plurality of RFID electronic tags, the RFID electronic tags are attached to a sample box, each wireless radio frequency antenna corresponds to one of the RFID electronic tags of the response unit, the wireless radio frequency antennas are matched with the radio frequency identification unit, and the central processing unit and a cloud server compare different RFID electronic tags.
The central processing unit adopts an embedded microprocessor, controls the antenna control module and the radio frequency identification module downwards, communicates with the cloud server, receives a cloud server instruction, performs front-end processing on received data and then sends the data to the cloud server.
The antenna control module is a general radio frequency switch, and the radio frequency switch receives and decodes a control signal of the central processing unit and controls the on-off of any radio frequency unit at any moment.
The radio frequency identification module is an RFID reader-writer, multi-antenna reading and writing according to positions and single-antenna reading and writing at specified positions are designed, and the antenna control module is matched for polling operation.
The radio frequency unit is designed into a matrix type, namely a matrix RFID antenna, and is controlled by an antenna control module, antennas at different positions have no crosstalk, so that the RFID tag can be accurately positioned, the radio frequency antenna adopts a UHF (ultra high frequency) band antenna and is designed into a near field antenna, and the interference among the antennas is reduced.
The RFID electronic tag of the response unit is the identification characteristic of the biological sample, adopts EPC-C1-G2 global uniform coding and has a global unique identification code.
A management method of an RFID-based biological sample library intelligent management system is characterized in that sample boxes and sample test tubes in a biological sample library are bound with RFID electronic tags; the user communicates with the cloud server through the terminal equipment; a portable sample box scanner and a sample checking instrument which are manufactured based on the RFID technology are connected with a cloud server; the user controls the RFID reader-writer to work in the terminal equipment, the antenna control module controls the on-off of the wireless radio frequency antenna according to the position by designing the matrix RFID antenna, the radio frequency identification module sends a reading instruction to read the RFID electronic tag attached to the biological sample, and the central processing unit collects and obtains biological sample data with position information so as to realize the functions of checking and positioning the biological sample through the RFID technology.
A management method of an RFID-based biological sample library intelligent management system comprises the following specific steps:
1) a user logs in the terminal equipment, and the cloud server records an operator according to login information;
2) recording:
a) a user uses the terminal equipment to control the sample checking instrument to enter a recording working mode;
b) placing a sample to be recorded at a recording position;
c) the characteristic code of the sample to be input is collected by a sample checking instrument and reflected on the terminal equipment through a cloud processor;
d) the user records the sample information to be input in the database corresponding to the feature code;
e) placing a sample box into which the sample needs to be placed in a reading area by a user;
f) reading the characteristic code of the sample box and recording the position of the sample placed in the sample box;
g) the recording process is finished;
3) checking:
a) a user controls the sample checking instrument to enter a checking working mode by using the terminal equipment;
b) placing the sample cartridge entirely in the sample inventorying instrument;
c) firstly, reading a sample box feature code and confirming sample box information;
d) entering a polling working mode, and reading the feature codes of the samples in the sample box according to the positions;
e) after reading, matching the read feature codes with original data of the database, and reflecting the feature codes to a display interface of the equipment terminal;
f) judging an abnormal condition and feeding back to a user;
g) finishing the checking process;
4) searching:
a) a user inputs a search condition in terminal equipment;
b) the system gives the position information of the sample according to the database;
c) according to the information, the user firstly uses the portable sample box scanner to scan the sample box where the sample is located;
d) placing the corresponding sample box into a sample counting instrument reading area, and reading the specified position to confirm whether the sample at the position is the required sample;
d) the search process ends.
When a user searches, uses, puts back and shifts the biological sample, the user needs to perform inventory before warehousing again and record operation in the system so as to ensure the correctness of the biological sample data.
The RFID electronic tag has the capability of normally working after resisting high-dose gamma radiation, and can keep the function after the biological sample tube is sterilized for medical use.

Claims (10)

1. An intelligent management system of a biological sample library based on RFID is characterized in that: the portable sample box scanning system comprises a cloud server, terminal equipment connected to the cloud server, a portable sample box scanner and a sample checking instrument, wherein the portable sample box scanner is used for scanning a sample box, and the sample checking instrument is used for checking sample test tubes in the sample box; the sample checking instrument comprises a central processing unit, an antenna control module and a radio frequency identification module which are connected to the central processing unit, a radio frequency unit which is connected to the antenna control module and the radio frequency identification module, and a response unit which is connected to the radio frequency unit, wherein the radio frequency unit comprises a plurality of wireless radio frequency antennas, the response unit comprises a plurality of RFID electronic tags, the RFID electronic tags are attached to a sample box, each wireless radio frequency antenna corresponds to one of the RFID electronic tags of the response unit, the wireless radio frequency antennas are matched with the radio frequency identification unit, and the central processing unit and a cloud server compare different RFID electronic tags.
2. The intelligent management system for biological sample bank based on RFID as claimed in claim 1, wherein: the central processing unit adopts an embedded microprocessor, controls the antenna control module and the radio frequency identification module downwards, communicates with the cloud server, receives a cloud server instruction, performs front-end processing on received data and then sends the data to the cloud server.
3. The intelligent management system for biological sample bank based on RFID as claimed in claim 1, wherein: the antenna control module is a general radio frequency switch, and the radio frequency switch receives and decodes a control signal of the central processing unit and controls the on-off of any radio frequency unit at any moment.
4. The intelligent management system for biological sample bank based on RFID as claimed in claim 1, wherein: the radio frequency identification module is an RFID reader-writer, multi-antenna reading and writing according to positions and single-antenna reading and writing at specified positions are designed, and the antenna control module is matched for polling operation.
5. The intelligent management system for biological sample bank based on RFID as claimed in claim 1, wherein: the radio frequency unit is designed into a matrix type, namely a matrix RFID antenna, and is controlled by an antenna control module, antennas at different positions have no crosstalk, so that the RFID tag can be accurately positioned, the radio frequency antenna adopts a UHF (ultra high frequency) band antenna and is designed into a near field antenna, and the interference among the antennas is reduced.
6. The intelligent management system for biological sample bank based on RFID as claimed in claim 1, wherein: the RFID electronic tag of the response unit is the identification characteristic of the biological sample, adopts EPC-C1-G2 global uniform coding and has a global unique identification code.
7. A management method of the RFID-based biological sample bank intelligent management system according to claim 1, wherein: binding a sample box and a sample test tube in a biological sample library with an RFID electronic tag; the user communicates with the cloud server through the terminal equipment; a portable sample box scanner and a sample checking instrument which are manufactured based on the RFID technology are connected with a cloud server; the user controls the RFID reader-writer to work in the terminal equipment, the antenna control module controls the on-off of the wireless radio frequency antenna according to the position by designing the matrix RFID antenna, the radio frequency identification module sends a reading instruction to read the RFID electronic tag attached to the biological sample, and the central processing unit collects and obtains biological sample data with position information so as to realize the functions of checking and positioning the biological sample through the RFID technology.
8. The method for managing an intelligent management system for a biological sample library based on RFID as claimed in claim 7, wherein: the method comprises the following specific steps:
1) A user logs in the terminal equipment, and the cloud server records an operator according to login information;
2) Recording:
a user uses the terminal equipment to control the sample checking instrument to enter a recording working mode;
placing a sample to be recorded at a recording position;
the characteristic code of the sample to be input is collected by a sample checking instrument and reflected on the terminal equipment through a cloud processor;
the user records the sample information to be input in the database corresponding to the feature code;
placing a sample box into which the sample needs to be placed in a reading area by a user;
reading the characteristic code of the sample box and recording the position of the sample placed in the sample box;
the recording process is finished;
3) Checking:
a user controls the sample checking instrument to enter a checking working mode by using the terminal equipment;
placing the sample cartridge entirely in the sample inventorying instrument;
firstly, reading a sample box feature code and confirming sample box information;
entering a polling working mode, and reading the feature codes of the samples in the sample box according to the positions;
after reading, matching the read feature codes with original data of the database, and reflecting the feature codes to a display interface of the equipment terminal;
judging an abnormal condition and feeding back to a user;
finishing the checking process;
4) Searching:
a user inputs a search condition in terminal equipment;
the system gives the position information of the sample according to the database;
according to the information, the user firstly uses the portable sample box scanner to scan the sample box where the sample is located;
placing the corresponding sample box into a sample counting instrument reading area, and reading the specified position to confirm whether the sample at the position is the required sample;
the search process ends.
9. The method for managing an intelligent management system for a biological sample library based on RFID as claimed in claim 8, wherein: when a user searches, uses, puts back and shifts the biological sample, the user needs to perform inventory before warehousing again and record operation in the system so as to ensure the correctness of the biological sample data.
10. The method for managing an intelligent management system for a biological sample library based on RFID as claimed in claim 7, wherein: the RFID electronic tag has the capability of normally working after resisting high-dose gamma radiation, and can keep the function after the biological sample tube is sterilized for medical use.
CN202011245826.6A 2020-11-10 2020-11-10 RFID-based biological sample library intelligent management system and management method thereof Pending CN113762435A (en)

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CN114663025A (en) * 2022-03-31 2022-06-24 武汉博特睿达智能科技有限公司 Biological sample library management system and method based on batch identification equipment
CN115489888A (en) * 2022-08-09 2022-12-20 中国人民解放军总医院 Intelligent management and storage all-in-one machine for biological sample resources
CN115936033A (en) * 2022-12-31 2023-04-07 苏州药明康德新药开发有限公司 Automatic sample checking system
CN115936033B (en) * 2022-12-31 2024-01-30 苏州药明康德新药开发有限公司 Automatic sample checking system
CN117132061A (en) * 2023-08-28 2023-11-28 阿坝藏族羌族自治州食品药品检验研究中心 System and method for managing traditional Chinese medicine specimen data based on wireless radio frequency
CN117132061B (en) * 2023-08-28 2024-03-08 阿坝藏族羌族自治州食品药品检验研究中心 System and method for managing traditional Chinese medicine specimen data based on wireless radio frequency

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