CN113758869A - Blood tissue chip preparation method, storage medium and electronic equipment - Google Patents
Blood tissue chip preparation method, storage medium and electronic equipment Download PDFInfo
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- CN113758869A CN113758869A CN202110936626.3A CN202110936626A CN113758869A CN 113758869 A CN113758869 A CN 113758869A CN 202110936626 A CN202110936626 A CN 202110936626A CN 113758869 A CN113758869 A CN 113758869A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G16H10/00—ICT specially adapted for the handling or processing of patient-related medical or healthcare data
- G16H10/60—ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H40/00—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices
- G16H40/20—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the management or administration of healthcare resources or facilities, e.g. managing hospital staff or surgery rooms
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Abstract
The invention relates to the field of biochips, in particular to a preparation method of a blood tissue chip, which comprises the steps of obtaining a tissue sample, processing the tissue sample to prepare a donor wax block; slicing the donor wax block to prepare a dyed slice; acquiring a donor wax block and a corresponding staining section according to the correlation information; marking the range of lesion tissues aiming at pathological diagnosis of the stained section; preparing an acceptor wax block, extracting a pathological wax block tissue core from the donor wax block according to the marked pathological tissue range, and implanting the donor wax block into the acceptor wax block according to a set rule; and treating the receptor wax block to obtain the blood tissue chip. In the process of making a blood sample into a tissue chip, the donor wax block and the stained section are searched according to the associated information, so that the technical problems of long time consumption and errors caused by human factors during sample matching are solved, and the technical effect of improving the matching efficiency is achieved.
Description
The technical field is as follows:
the invention relates to the field of biochips, in particular to a blood tissue chip preparation method, a storage medium and electronic equipment.
Background art:
tissue chips, also called tissue microarrays, are an important branch of biochip technology, and are tissue sections produced by arranging tens to thousands of small tissues in order on a single slide.
During the chip manufacturing process, donor tissues are firstly embedded into a donor wax block, tissues of a target area are taken out from the donor wax block by using a sampling needle according to requirements, and different tissue points are arranged on a receptor wax block manufactured in advance according to the requirements of a customer. Then the arranged tissue points and the receptor wax block are fused into a wax block, and then subsequent slicing is carried out. The preparation of blood tissue chips is not widely used and is inefficient in the matching of stained sections with wax blocks.
The invention is provided in view of the above.
The invention content is as follows:
the present invention is directed to a method for preparing a blood tissue chip to solve at least one of the above problems.
The invention aims to provide a preparation method of a blood tissue chip, which comprises the steps of obtaining a tissue sample, processing the tissue sample and preparing a donor wax block; slicing the donor wax block to prepare a dyed slice; acquiring a donor wax block and a corresponding staining section according to the correlation information; marking the range of lesion tissues aiming at pathological diagnosis of the stained section; preparing an acceptor wax block, extracting a pathological wax block tissue core from the donor wax block according to the marked pathological tissue range, and implanting the donor wax block into the acceptor wax block according to a set rule; and treating the receptor wax block to obtain the blood tissue chip.
Further, the step of obtaining a donor wax block and a corresponding stained section comprises terminal reading a first tag of a donor wax block cassette used to embed the tissue sample; retrieving medical record information associated with the sample organization and associating the medical record information with the first label; the terminal reads a second label on a slice box for presenting the stained slice; associating the first label with the second label; and accumulating the donor wax blocks and the dyed sections to generate associated information.
Further, the step of obtaining the donor wax block and the corresponding stained section comprises receiving a batch of medical record information searching instructions; and according to the medical record information searching instruction, generating a region set where the donor wax block and the dyed section to be acquired are located.
Further, after the step of generating the region set where the donor wax block and the stained section to be obtained are located, traversing all the region sets, and identifying the number attribute of the region sets; and calling a set rule, and carrying out sampling sequence arrangement on the region set according to the set rule.
Further, the step of generating a set of regions where the donor wax block, the stained section, is to be obtained comprises: acquiring an extraction instruction; traversing all the area sets, and identifying the number attribute of the area sets; acquiring power device information corresponding to the serial number attribute in the area set; the power device receives the server information to open the storage space for the donor wax blocks and/or stained sections to be removed.
Further, the step that the power device receives information of the server to open a storage space for storing the donor wax block and/or the dyed slice to be taken out further comprises the step of judging whether a first induction signal sent by the signal transmitter is received, wherein the first induction signal is used for identifying whether the storage space is opened or not, and identifying the transmitter number of the signal transmitter and marking the transmitter number as a checking transmitter under the condition that the judgment is negative; traversing the database, and matching a first label and a second label corresponding to the serial number of the checking transmitter; and carrying out fault marking on the first label and the second label on a terminal display interface.
Further, the step of judging whether to receive a second sensing signal sent by the signal emitter after receiving the sensing information sent by the signal emitter is further included in the step of judging whether to receive the second sensing signal sent by the signal emitter under the condition that the judgment is yes, wherein the second sensing signal is used for identifying the opening degree of the storage space; and under the condition that the second sensing signal is not received, identifying the emitter number of the signal emitter and marking as a checking emitter.
Further, the step of processing the receptor wax block to obtain the blood tissue chip further comprises a terminal reading a third label on a receptor wax block box, wherein the receptor wax block box is used for containing the receptor wax block; associating the third label with the first label.
The present invention also protects a storage medium having stored therein processor-executable instructions that are loaded by one or more processors to perform the above-described method of processing an executable file.
The invention also protects an electronic device, which comprises a processor and a memory, wherein the memory stores a computer program, and the processor is used for executing the processing method of the executable file by calling the computer program.
In conclusion, the invention has the following beneficial effects:
1. by searching the donor wax block and the stained section according to the associated information in the process of making the blood sample into the tissue chip, the technical problems of long time consumption and error caused by human factors in sample matching are solved, and the technical effect of improving the matching efficiency is achieved.
2. By generating the area set on line and carrying out sampling sequence arrangement on the area set, the technical problems that workers are complex and prone to error when searching are solved, and the technical effect of improving searching efficiency and accuracy is achieved.
3. The arrangement of the first induction signal and the second induction signal solves the technical problem that the search result is adversely affected due to machine faults, and the technical effect of improving the search accuracy is achieved.
Description of the drawings:
in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of one embodiment of the present invention;
FIG. 2 is a schematic view of one embodiment of a wax block cassette;
FIG. 3 is a schematic view of one embodiment of a microtome cassette;
FIG. 4 is a flow chart of another embodiment of the present invention;
FIG. 5 is a schematic diagram of a terminal interface according to the present invention;
FIG. 6 is a schematic view of a storage cabinet according to an embodiment of the present invention;
FIG. 7 is another schematic illustration of a terminal interface according to the present invention;
FIG. 8 is a schematic view of an embodiment of a drawer connection;
FIG. 9 is a schematic view of one embodiment of a drawer push arrangement;
FIG. 10 is a schematic view of an embodiment of a drawer bulkhead;
FIG. 11 is a schematic view of one embodiment of an access opening;
FIG. 12 is a schematic view of a drawer side connection;
FIG. 13 is a partial perspective view of a drawer side connection.
Fig. 14 is a schematic diagram of infrared sensor signal emission.
Description of reference numerals:
the technical scheme of the invention can be more clearly understood and explained by combining the embodiment of the invention through the reference sign description.
11-rear fixing plate, 111-fixing rod, 12-side fixing plate, 1211-upper protecting plate, 1212-side protecting plate, 1213-track plate, 1214-blocking body, 13-door leaf, 21-second power mechanism, 22-containing device, 23-separating plate, 231-placing hole, 24-access opening, 25-placing platform, 26-connecting plate, 27-third connecting piece, 28-supporting plate, 29-drawer, 3-propelling device, 31-first power mechanism, 32-first transmission body, 33-second transmission body, 34-first connecting piece, 35-second connecting piece, 4-shell and 5-third connecting piece; 6-infrared sensor, 61-emitted light, 62-reflected light, 64-black fixture.
The specific implementation mode is as follows:
the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in this specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.
The present invention will be described in detail below by way of examples.
Referring to fig. 1, 2 and 3, the invention provides a method for preparing a blood tissue chip, comprising the following steps:
s100: obtaining a tissue sample, and processing the tissue sample to prepare a donor wax block;
s200: slicing the donor wax block to prepare a dyed slice;
s300: acquiring a donor wax block and a corresponding staining section according to the correlation information;
s400: marking the range of lesion tissues aiming at pathological diagnosis of the stained section;
s500: preparing an acceptor wax block, extracting a pathological wax block tissue core from the donor wax block according to the marked pathological tissue range, and implanting the donor wax block into the acceptor wax block according to a set rule;
s600: and treating the receptor wax block to obtain the blood tissue chip.
By adopting the scheme, the blood can be processed to be prepared into the blood clot so as to be prepared into the stained section, the blood clot tissue is marked in the external examination specimen of the pathology department and is difficult to prepare into high-quality pathological sections, and the sections are fragile and hard firstly and fragile secondly, so that the sections are incomplete; thirdly, the sheet is easy to be taken off, and the dyed slices and the donor wax block can be prepared by adopting the following method, particularly, the fixation is carried out, and the quick fixation solution is preferably selected: 8Oml parts of 95 ethanol, 15ml of formaldehyde and 5ml of glacial acetic acid, wherein the fixing time is not too long for 1 hour; dehydrating with acetone directly, wherein the dehydration with ethanol can make the tissue relatively hard, and the dehydration time is controlled within 2 h; wax dipping, wherein the wax dipping temperature is preferably controlled to be 56 ℃, excessive shrinkage of blood clot tissues can occur due to overhigh temperature, and the wax dipping time is controlled to be about 1h so as to avoid the influence on tabletting due to overhigh and fragile tissues; slicing, wherein the thickness is 4-5 m conventionally, the blood clot tissue is preferably 3m, the adhesion force between the thin slice and the glass slide is strong, the slice is not easy to fall off, and the wax block can be slightly blown to soften the tissue and cut into complete slices without breaking or folding during continuous slicing; and (3) dyeing, wherein the blood clot tissue contains a large amount of red blood cells and the tissue components are few, so that the cut blood clot tissue section is preferably dyed separately from other tissue sections, the hematoxylin staining time is longer, and the eosin staining time is shorter, so that the dyeing results are well compared, and the microscopic examination is convenient.
And determining corresponding tissue points corresponding to the donor wax block according to the marked lesion tissue range, taking out tissue cores of the corresponding tissue points through a sampling and lofting instrument, implanting the tissue cores into the receptor wax block, and slicing the receptor wax block to prepare the blood tissue chip.
The preparation of the receptor wax block can drill the wax block through drilling equipment, the drilling equipment can comprise a base, a support, a Y-axis moving device, an X-axis moving device, a Z-axis moving device, a drilling motor, a workbench, a controller and a drill bit, the support is connected with the base, the Y-axis moving device is connected with the base, the workbench is connected with the Y-axis moving device, the X-axis moving device is connected with the support, the Z-axis moving device is connected with the X-axis moving device, the drilling motor is connected with a Z-axis moving component, the drill bit is connected with the drilling motor, the Y-axis moving device, the X-axis moving device, the Z-axis moving device and the drilling motor are electrically connected with the controller, a user inputs instructions through the controller, and the controller controls the Y-axis moving device, the X-axis moving device and the Z-axis moving device to move and the drilling motor to rotate, x axle mobile device, Z axle mobile device drive the drill bit and remove at X axle, Z axle direction, Y axle mobile device drives workstation Y axle and removes, drilling motor drives the drill bit and rotates, place tissue chip wax stone on the workstation, realize the drilling to the accurate location of tissue chip wax stone, the three-dimensional direction controllable regulation of drilling position.
Referring to fig. 4, 5, and 6, the steps of obtaining a donor wax block and a corresponding stained section include terminal reading of a first label of a donor wax block cassette used to embed the tissue sample; retrieving medical record information associated with the sample organization and associating the medical record information with the first label; the terminal reads a second label on a slice box for presenting the stained slice; associating the first label with the second label; and accumulating the donor wax blocks and the dyed sections to generate associated information.
With the above scheme, the first label may include donor wax block box information, specifically, may be storage location information of the donor wax block box, for example, the first label information may be "a 03 cabinet 01 zone 01 row 01 column"; the medical record information of the sample organization can comprise source attribution information such as patient name, age and the like; the second label information may include information on the location where the slide cassette is stored, and for example, the second label information may be "a 03 row 02 in the cabinet 02 area 02". The first label and the second label can be arranged in a bar code or a two-dimensional code form, and the surface packaging prevents the first label and the second label from being corroded when soaked. The medical record information of the sample organization can be pre-input into an information base. Specifically, when a worker prepares the donor wax block, the worker can scan the first label by holding a mobile phone and other terminal equipment, and then a corresponding interface can be popped up, wherein the information of each first label is different. The staff can know the organization medical record information embedded into the donor wax block in advance, then the staff can find the medical record information which is the same as the organization medical record information which is known in advance in the options of the interface through attribute screening, for example, screening is carried out through the name Zhang III, and the medical record information is input, so that each piece of medical record information corresponds to the corresponding first label, and information interconnection and binding are realized. When the terminal reads the second label, a corresponding selection interface is popped up, the first label is correlated with the second label by searching the information of the first label in the information base, namely, the medical record information, the first label information and the second label information are jointly bound, and the information is uploaded to the cloud server for recording and then is stored. When the staff needs to search for the dyed slices or donor wax blocks of corresponding patients, the one-to-one correspondence relationship between the donor wax block boxes and the slice boxes in the storage space can be realized by inputting the attributes in the medical record information at the terminal, and the accurate positioning is realized.
The storage space is provided in the form of a storage cabinet or in the form of a drawer 29 in a storage cabinet.
In the current preparation process, an offline recording mode is usually adopted, corresponding errors of the three are easy to occur due to negligence of workers and improper operation, and the situation that the search is not matched with the actual search due to human errors during the search occurs. The technical scheme has the technical problems of improving the preparation efficiency, the storage efficiency and the storage accuracy.
Referring to fig. 5, the steps of obtaining donor wax blocks and corresponding stained sections include:
receiving batch medical record information searching instructions;
and according to the medical record information searching instruction, generating a region set where the donor wax block and the dyed section to be acquired are located.
By adopting the scheme, in the actual preparation process, a plurality of groups of donor wax blocks and dyeing slices are required to be taken out in sequence, and because the taken donor wax blocks and the dyeing slices are not necessarily adjacent to each other in the storage position, the working personnel need to frequently take the donor wax blocks and the dyeing slices from different storage spaces, so that the material taking efficiency is greatly reduced. Above-mentioned scheme, through the case history information that will input in batches, can confirm the information of the donor wax piece box and the slice box of waiting to acquire, through match the information of the two with storage space's in the information bank information, can generate all the information of the donor wax piece box and the slice box of waiting to acquire in the same storage space in batches at the display interface, the staff passes through above-mentioned information, can once only get the donor wax piece box or the slice box in the same storage space completely, remove other storage spaces and draw materials again, sampling efficiency has been promoted. The reference of the region set can be preset, and a single cabinet can be used as a reference unit or each layer in the cabinet can be used as a reference unit according to the sizes of different storage spaces.
Referring to fig. 7, the step of generating a set of regions where the donor wax pieces, stained sections, are to be acquired comprises:
traversing all the area sets, and identifying the number attribute of the area sets;
and calling a set rule, and carrying out sampling sequence arrangement on the region set according to the set rule.
By adopting the scheme, the number attribute can be Arabic numbers or letters on numbers, the established rule can be that the numbers of the storage spaces are arranged from large to small or from small to large, and the layers can be arranged in the order of top, middle and bottom, for example, the displayed region sets are arranged in the order of "A01 cabinet 01 area 01 row 01 column", "A01 cabinet 01 area 02 row 01 column", "A01 cabinet 02 area 01 row 01 column", "A02 cabinet 02 area 02 row 01 column", and "A03 cabinet 03 area 02 row 01 column", because the actual storage spaces are also arranged in the order of the numbers, a worker can conveniently take out all the donor wax blocks and the dyeing slices at one time in the adjacent layers or the adjacent storage spaces without frequently switching different storage spaces, and the working efficiency is improved.
The step of generating the region set where the donor wax block and the stained section to be obtained are located comprises the following steps:
acquiring an extraction instruction;
traversing all the area sets, and identifying the number attribute of the area sets;
acquiring power device information corresponding to the serial number attribute in the area set;
the power device receives the server information to open the storage space for the donor wax blocks and/or stained sections to be removed.
By adopting the scheme, each storage space is provided with a power device capable of being automatically opened, such as a motor-driven power device or a hydraulic power device, the server can send an opening instruction to the cabinet body or the drawer 29 containing the region collection information after receiving the region collection information, the opened storage space for opening the storage space for storing the donor wax blocks and/or the dyeing slices to be taken out does not necessarily represent a cabinet door, and the storage space can also be in the forms of pulling the drawer 29 open, opening the upper cover and the like, at the moment, the corresponding storage space is opened without the comparison terminal by the staff one by one, the drawer 29 of the corresponding storage space can be opened in an automatic opening mode, and the staff can be closed after taking one drawer 29 each time until all the drawers are taken out. The scheme reduces human errors of workers and improves the working efficiency.
Fig. 6, 8-13 below illustrate a storage cabinet for storing wax blocks or shifting pieces, which includes a cabinet body including a side fixing plate 12 and a drawer connected to the side fixing plate 12; the drawer comprises a second power device 21 arranged inside and a containing device 22 connected with the second power device 21, wherein the containing device 22 can move under the action of the second power device 21. By adopting the scheme, the second power device can adopt a servo motor or a hydraulic transmission mechanism, the servo motor is adopted in the embodiment, the linkage rod can be installed on the motor shaft of the servo motor, the accommodating device is arranged on the upper side of the linkage rod and can adopt connection modes such as screw connection or buckle connection and the like, the servo motor can be connected with the control mechanism and a power supply to control the rotation angle, the upper part of the accommodating device can be provided with an opening for placing the tissue chip wax block, when the servo motor operates at an upward rotation angle, the corresponding accommodating device can move upwards, so that a worker can find the corresponding tissue chip wax block in an automatic control mode, and the time of the worker is greatly saved.
The servo motors can be arranged on one side of the inner space of the drawer, and a plurality of servo motors are arranged along the axial direction of the drawer as required; the setting direction of trace can follow the radial setting of drawer, can set up a plurality ofly in quantity along the axial direction of drawer to set up suitable interval, can not produce the hindrance between every accommodate device with the assurance.
The drawer comprises a partition plate 23 arranged above the second power device 21, and a placing hole 231 matched with the accommodating device 22 is formed in the partition plate 23.
With the above technical scheme, the partition 23 can protect the second power device 21, the partition 23 can be integrally formed with the drawer and can be detachably connected with the drawer, and the placing hole 231 is formed according to the shape and size of the accommodating device 22, so that the accommodating device 22 cannot be obstructed when moving upwards.
And an access opening 24 corresponding to the second power device 21 is formed in the side surface of the drawer.
By adopting the scheme, the second power device 21 can be overhauled or nursed without taking down the partition plate 23 through the access hole 24, and the operation is greatly facilitated.
The tissue chip wax block storage cabinet further comprises a rear fixing plate 11 and a pushing device 3 connected with the rear fixing plate 11, wherein the pushing device 3 comprises a first power device 31 with one end connected with the drawer, a first transmission body 32 with one end connected with the other end of the first power device 31, and a second transmission body 33 connected with the first transmission body 32, one end of the second transmission body 33 is connected with the rear fixing plate 11, and the first transmission body 32 can be displaced relative to the second transmission body 33 under the driving of the first power device 31.
By adopting the scheme, the back surface of the tissue chip wax block storage cabinet is provided with the rear fixing plate 11, the fixing plate 11 shown in fig. 4 is a part of the integral rear fixing plate 11, a plurality of drawers can be arranged in the cabinet body, the specific specification and shape can be customized according to specific requirements, and the rear fixing plate 11 can be arranged into a whole panel or a single plate corresponding to each drawer independently; the side fixing plates 12 may be outer plates at both sides of the cabinet body or inner plates with internal spaces parallel to the outer plates; the pushing device 3 is used for providing a reaction supporting force to push out the drawer by taking the rear fixing plate 11 as a supporting surface, the first power device 31 can be connected with a power supply and a control mechanism at the moment, the power supply provides electric energy, the control mechanism can output corresponding programs to open different drawers according to the selection of a worker, and the drawers can be automatically closed after the worker takes out corresponding wax blocks; wherein the second transmission body 33 is a stationary part, and the first transmission body 31 is a part that is relatively displaced with respect to the second transmission body 33, and at this time the drawer is also displaced in connection with the pushing device 3, thereby achieving opening and closing.
An internal thread is arranged on the inner side of the first transmission body 32, and an external thread matched with the internal thread is arranged on the outer side of the second transmission body 33.
With the above-mentioned solution, the first power device 31 may be a dc motor or a heat conducting mechanism, etc., and may drive the first transmission body 32 to rotate, and may be disposed in the protective casing 4, the first power device 31 may be connected to the first transmission body 32 through a second connecting member 35, the first transmission body 32 and the second transmission body 33 may be disposed in a cylindrical shape, the outer diameter of the first transmission body 32 is larger than the outer diameter of the second transmission body 33, at this time, the second transmission body 33 is kept still because the second transmission body 33 is connected to the rear fixing plate 11, and the exposed length of the second transmission body 33 is longer and longer under the mutual fit of the internal and external threads, at this time, the first transmission body 32 moves, and because the first power device 31 is connected to the first transmission body 32 and the first power device 31 is connected to the drawer, and then the drawer is driven to be opened, and when the drawer needs to be closed, the first power device 31 can rotate in the opposite direction, so that the drawer is closed.
The same effect can be achieved by providing the first transmission body 32 with a smaller outer diameter than the second transmission body 33, providing an external thread on the outside of the first transmission body 32 and an internal thread on the inside of the second transmission body 33.
The rear fixing plate 11 is provided with a fixing rod 111, and one end of the second transmission body 33 is provided with a first connecting member 34 connected with the fixing rod 111.
By adopting the scheme, the connecting mode of the rear fixing plate 11 and the second transmission body 33 is provided, the fixing rod is detachably connected with the fixing plate 11 and can be in a screwed connection or a buckled connection mode, the first connecting piece 34 is detachably connected with the second transmission body 33 and can be detached to be replaced independently without being integrally replaced when parts are damaged and need to be replaced through the screwed connection or the buckled connection mode.
The first power device 31 is connected with the rear side of the drawer.
Adopt above-mentioned scheme, provide a connected mode, can with first power device 31 through third connecting piece 5 with the drawer rear side is connected, has reduced the usage space, sets up rationally.
In other embodiments of the present invention, referring to fig. 4, a placing platform 25 is disposed at the rear side of the drawer, and the first power device 31 is disposed on the placing platform 25.
By adopting the scheme, another connection mode is provided, and the placing platform 25 is connected with the rear side of the drawer and is used for supporting the first power device 31.
A connecting plate 26 is arranged below the placing platform 25, and an accommodating space is formed between the placing platform 25 and the connecting plate 26.
By adopting the above scheme, the accommodating space formed between the placing platform 25 and the connecting plate 26 can be used for placing parts such as wires for connection, and the connecting space between the first power device 31 and the control mechanism and the connecting space between the second power device 21 and the control mechanism are smooth and reasonable, so that the failure occurrence rate is reduced.
The side fixing plate 12 is provided with a moving track, the side of the drawer is provided with a third connecting piece 27 matched with the moving track, and the third connecting piece 27 can move relatively along the moving track.
With the above scheme, the moving rail is fixed on the side fixing plate 12 and is used for providing conditions for opening and closing the drawer, the moving rail may include a rail plate 1213, a side protection plate 1212 having one side connected to the rail plate 1213, and an upper protection plate 1211 connected to the other side of the side protection plate 1212, the third connecting member 27 may be a wheel or a slider, when the drawer moves, the third connecting member 27 may move on the lower rail plate 121, and the side protection plate 1212 may prevent the third connecting member 27 from falling out and play a role in protection.
The side of the drawer is also provided with a supporting plate 28 which is matched with the upper protective plate 1211, and the supporting plate 28 is used for supporting the upper protective plate 1211, so that the drawer can run more stably in the moving process; a blocking body 1214 is further arranged at one end of the side guard plate 1212, and the blocking body 1214 can block the movement of the third connecting piece 27 so as to prevent the drawer from sliding out of the storage cabinet, thereby playing a role in protection; the drawer should include a panel 29 for protecting the stored items.
The first power device 31 and the second power device 21 are in signal connection with a central control system, and the central control system or the server can control the corresponding power devices to work according to instructions.
Referring to fig. 6, the step of the power device receiving the server information to open the storage space for the donor wax block and/or the stained section to be taken out further comprises:
judging whether a first induction signal sent by a signal transmitter is received or not, wherein the first induction signal is used for identifying whether a storage space is opened or not;
under the condition that the judgment is negative, identifying the emitter number of the signal emitter, and marking as an inspection emitter;
traversing the database, and matching a first label and a second label corresponding to the serial number of the checking transmitter;
and carrying out fault marking on the first label and the second label on a terminal display interface.
Adopt above-mentioned scheme, signal transmitter can be infrared ray sensor 6, installs light transmitting tube, light receiver tube on the infrared ray sensor 6, and every infrared ray sensor 6 all corresponds a drawer, and every infrared ray sensor 6 is installed by every drawer, and install on the cabinet body. When the drawer is not used, the drawer is closed, and the light emitting tube and the light receiving tube are in a closed state; after obtaining the extraction instruction, the first power device 31 pushes the drawer to be pushed out, after a certain time interval, the infrared sensor 6 is opened, the time interval can be set according to the time when the drawer is completely opened, the first sensing signal is information that the emitted light is irradiated by the side surface of the cabinet body to become reflected light and is received by the light receiving tube, if the first sensing signal is received, the drawer is proved to be pushed out, if the first sensing signal is not received, the drawer is not pushed away due to the power device or line connection and other problems, at the moment, the corresponding label information is displayed through the terminal again to enable the worker to learn the drawer which is not opened, so as to determine the donor wax block and the dyeing slice which cannot be taken out, and form a record to remind the worker to guarantee maintenance or overhaul, at the moment, for the drawer which is not normally opened, the worker may also choose to pull the pull apart manually. The scheme adopts intelligent sampling, has promoted sampling efficiency, has reduced the sample inaccuracy that just causes because of artificial error, and the accuracy of still keeping the sample when the staff forms the fault record and ensures breaking down simultaneously can also be helped in the acquisition of first sensing signal.
Referring to fig. 6 and 14, the step of determining whether the sensing information sent by the signal transmitter is received further includes:
if so, judging whether a second induction signal sent by a signal transmitter is received or not, wherein the second induction signal is used for identifying the opening degree of the storage space;
and under the condition that the second sensing signal is not received, identifying the emitter number of the signal emitter and marking as a checking emitter.
Adopt above-mentioned scheme, can be at this moment the drawer be close to infrared sensor one side curb plate, be close to the position of afterbody on, fixed black fixture 64 can be the sticker form, because of signal transmitter adopt infrared sensor 6 time, infrared ray shines black interface light can be absorbed, can't form the reflection and lead to signal receiver unable received signal, does not accept the second sensing signal this moment promptly. At the moment, the drawer is not completely opened, a fault occurs, the dyed slices or donor wax blocks on the inner side can not be taken out, and at the moment, label information is determined according to the number of the signal transmitter, so that the fault position is determined. The scheme adopts intelligent sampling, has promoted sampling efficiency, has reduced the sample inaccuracy that just causes because of artificial error, and acquisition of first sensing signal can also help the staff to form the fault record and simultaneously, still keeps the accuracy of sample when guaranteeing to break down.
Treating the receptor wax block to obtain the blood tissue chip, wherein the step of obtaining the blood tissue chip further comprises the following steps:
reading a third label on a receptor wax block box by the terminal, wherein the receptor wax block box is used for containing a receptor wax block;
associating the third label with the first label.
By adopting the scheme, the donor wax block box and the receptor wax block box are associated, so that the searching efficiency of workers can be improved.
The present invention also protects a storage medium having stored therein processor-executable instructions that are loaded by one or more processors to perform the above-described method of processing an executable file.
The invention also protects an electronic device, which comprises a processor and a memory, wherein the memory stores a computer program, and the processor is used for executing the processing method of the executable file by calling the computer program.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. The invention provides a preparation method of a blood tissue chip, which comprises the following steps:
obtaining a tissue sample, and processing the tissue sample to prepare a donor wax block;
slicing the donor wax block to prepare a dyed slice;
acquiring a donor wax block and a corresponding staining section according to the correlation information;
marking the range of lesion tissues aiming at pathological diagnosis of the stained section;
preparing an acceptor wax block, extracting a pathological wax block tissue core from the donor wax block according to the marked pathological tissue range, and implanting the donor wax block into the acceptor wax block according to a set rule;
and treating the receptor wax block to obtain the blood tissue chip.
2. The method for preparing a blood tissue chip according to claim 1, wherein the step of obtaining the donor wax block and the corresponding stained section comprises:
terminal reading a first label of a donor wax block cassette used to embed a tissue sample;
retrieving medical record information associated with the sample organization and associating the medical record information with the first label;
the terminal reads a second label on a slice box for presenting the stained slice;
associating the first label with the second label;
and accumulating the donor wax blocks and the dyed sections to generate associated information.
3. The method of claim 2, wherein the step of obtaining the donor wax block and the corresponding stained section comprises:
receiving batch medical record information searching instructions;
and according to the medical record information searching instruction, generating a region set where the donor wax block and the dyed section to be acquired are located.
4. The method for preparing a blood tissue chip according to claim 3, wherein the step of generating a set of regions where the donor wax block and the stained section to be obtained are located comprises:
traversing all the area sets, and identifying the number attribute of the area sets;
and calling a set rule, and carrying out sampling sequence arrangement on the region set according to the set rule.
5. The method for preparing a blood tissue chip according to claim 3, wherein the step of generating a set of regions where the donor wax block and the stained section to be obtained are located comprises:
acquiring an extraction instruction;
traversing all the area sets, and identifying the number attribute of the area sets;
acquiring power device information corresponding to the serial number attribute in the area set;
the power device receives the server information to open the storage space for the donor wax blocks and/or stained sections to be removed.
6. The method for preparing a blood tissue chip according to claim 5, wherein the step of the power device receiving the server information to open the storage space for the donor wax block and/or the stained section to be taken out further comprises:
judging whether a first induction signal sent by a signal transmitter is received or not, wherein the first induction signal is used for identifying whether a storage space is opened or not;
under the condition that the judgment is negative, identifying the emitter number of the signal emitter, and marking as an inspection emitter;
traversing the database, and matching a first label and a second label corresponding to the serial number of the checking transmitter;
and carrying out fault marking on the first label and the second label on a terminal display interface.
7. The method of claim 5, wherein the step of determining whether the sensing information sent by the signal transmitter is received further comprises:
if so, judging whether a second induction signal sent by a signal transmitter is received or not, wherein the second induction signal is used for identifying the opening degree of the storage space;
and under the condition that the second sensing signal is not received, identifying the emitter number of the signal emitter and marking as a checking emitter.
8. The method of any one of claims 3 to 7, wherein the step of treating the receptor wax block to obtain the blood tissue chip further comprises:
reading a third label on a receptor wax block box by the terminal, wherein the receptor wax block box is used for containing a receptor wax block;
associating the third label with the first label.
9. A storage medium having stored therein processor-executable instructions, which are loaded by one or more processors to perform a method of processing an executable file as claimed in any one of claims 1 to 8.
10. An electronic device, characterized in that it comprises a processor and a memory, said memory storing a computer program, said processor being adapted to execute the method of processing an executable file according to any one of claims 1 to 8 by invoking said computer program.
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