CN113753381B - Novel coronavirus multiplex PCR (polymerase chain reaction) rapid detection kit - Google Patents

Novel coronavirus multiplex PCR (polymerase chain reaction) rapid detection kit Download PDF

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Publication number
CN113753381B
CN113753381B CN202111210449.7A CN202111210449A CN113753381B CN 113753381 B CN113753381 B CN 113753381B CN 202111210449 A CN202111210449 A CN 202111210449A CN 113753381 B CN113753381 B CN 113753381B
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China
Prior art keywords
reagent
groove
hole
pull rod
kit
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CN202111210449.7A
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CN113753381A (en
Inventor
蒋玉香
王熙婷
韩林秀
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Zhenjiang Jintai Medical Science Examination Laboratory Co ltd
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Zhenjiang Jintai Medical Science Examination Laboratory Co ltd
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02Internal fittings
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02Internal fittings
    • B65D25/10Devices to locate articles in containers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/38Devices for discharging contents
    • B65D25/52Devices for discharging successive articles or portions of contents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/02Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
    • B65D81/05Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents

Abstract

The invention relates to a novel coronavirus multiplex PCR (polymerase chain reaction) rapid detection kit, which comprises a kit body, a plurality of reagent holes arranged at the top of the kit body, a protection part arranged at the bottom of the reagent holes, a driving part arranged at the bottom of the protection part, and reagent tubes arranged in the reagent holes, wherein the reagent tubes are arranged in the reagent holes; a first through hole groove is formed in the bottom of the reagent hole, and when the reagent tube is inserted into the reagent hole and is in contact with the protection ball, the first through hole groove presses down two groups of sliding blocks to slide towards two sides along the sliding rod. According to the invention, the reagent tube is placed in the reagent hole, the reagent kit body is made of opaque materials, so that the light is prevented from influencing the detection reagent, the reagent tube can be protected by the arranged jacking column, the bottom wall of the reagent tube and the bottom wall of the reagent hole are prevented from generating devices to cause the internal detection reagent to shake, then the detection design can be jacked out from the inside of the reagent hole by the arranged driving part, and the detection reagent is convenient to take.

Description

Novel coronavirus multiple PCR rapid detection kit
Technical Field
The invention relates to the technical field of kits, in particular to a novel coronavirus multiplex PCR rapid detection kit.
Background
In chinese patent application No. 201720505514.1, a quick detect reagent box of PCR is disclosed, including gasbag, transparent material test tube, sealing plug, pipe syringe needle, mounting panel, lead screw, bearing ring, sucking disc and well hollow rod of getting, the gasbag lower extreme is equipped with the transparent material test tube of getting, the transparent material test tube lower extreme of getting is installed the pipe syringe needle, the transparent material test tube of getting sets up on the mounting panel, the sealing plug assembly is on the transparent material test tube of getting, and this design can take out reagent fast, also prevents the maloperation, the sucking disc upper end is equipped with well hollow rod, install the bearing ring on the well hollow rod, the bearing ring assembly is on the lead screw, inside the hollow rod extends into the well hollow rod of lead screw lower extreme, and the adjustable end box levelness of this design prevents that the intraductal reagent of reagent from spilling, and this reagent box convenient to use improves detection efficiency, and stability is good, and the reliability is high.
The detection kit is convenient to use, improves the detection efficiency, has good stability and high reliability, but the detection kit is inconvenient and quick to take out the detection reagent from the kit, so the problems are solved by designing a novel coronavirus multiplex PCR rapid detection kit.
Disclosure of Invention
The invention aims to provide a novel coronavirus multiplex PCR rapid detection kit.
In order to solve the above technical problems, the present invention provides a novel coronavirus multiplex PCR rapid detection kit, comprising
The kit comprises a kit body, a plurality of reagent holes arranged at the top of the kit body, a protection part arranged at the bottom of the reagent holes, a driving part arranged at the bottom of the protection part, and reagent tubes arranged in the reagent holes;
a first through hole groove is formed in the bottom of the reagent hole, a protection groove is formed in the bottom of the first through hole groove, the inner diameter of the first through hole groove is smaller than that of the reagent hole, and the inner diameter of the protection groove is larger than that of the reagent hole;
the protection part comprises a sliding rod arranged on the inner side wall of the protection groove, an expansion spring is sleeved on the sliding rod, two groups of sliding blocks are arranged on the surface of the sliding rod in a sliding mode, the tops of the sliding blocks are hinged with first hinged rods, and a protection ball is hinged between every two adjacent groups of the first hinged rods;
the protective ball protrudes from the first through hole groove, wherein
When the reagent pipe is inserted into the reagent hole and is contacted with the protective ball, the first through hole grooves press the two sets of sliding blocks to slide towards two sides along the sliding rod, so that the shaking of the internal detection liquid of the reagent hole is reduced.
Furthermore, the bottom of reagent pipe is provided with the constant head tank, the constant head tank is the semicircular arrangement, the protection ball is the spheroid setting, the protection ball is located the central point of reagent hole bottom puts, wherein
After the reagent tube is inserted into the reagent hole, the positioning groove wraps the top wall of the protective ball.
Furthermore, a plurality of pull rod grooves are formed in the bottoms of the two side walls of the kit body, and bottom grooves are formed in the adjacent pull rod grooves.
Furthermore, the driving part comprises a pull rod body arranged in the pull rod groove, a jacking block fixed at the top of the pull rod groove and a baffle body hinged between two adjacent groups of the pull rod grooves;
the front view section of the jacking block is in a right trapezoid shape, and the top wall of the baffle body is attached to the top wall of the bottom groove.
Furthermore, a plurality of baffles are arranged at the top of the kit body, the baffle body is arranged inside the baffles, and the jacking blocks isolate two adjacent groups of reagent holes.
Further, the bottom end of the baffle plate is communicated with the top wall of the bottom groove;
two groups of second hinged rods are hinged to the middle position of the pull rod body, the other ends of the two groups of second hinged rods are hinged to the bottom of the baffle body, and the other ends of the two groups of second hinged rods are hinged to the bottom of the baffle body
When the pull rod body is completely inserted into the pull rod groove, the two groups of second hinge rods are folded in a triangular mode, and the top end of the baffle body is ejected out of the pull rod groove.
Further, a second through hole groove is formed in the bottom of the protection groove and communicated with the bottom groove;
and a jacking column is installed at the top of the pull rod body, and the top end of the jacking column is positioned in the second through hole groove.
Furthermore, the jacking column is cylindrical, the inside of the jacking column is hollow, a vertical groove is formed in the inside of the jacking column, and a spherical block is fixed at the bottom of the jacking column, wherein
When the jacking block moves upwards, the first hinge rod is inserted into the vertical groove, and the jacking column penetrates through the first through hole groove to jack up the reagent tube.
Further, when the pull rod body is pulled outwards, the jacking block moves along with the pull rod body, and the inclined edge position of the jacking block is abutted against the spherical block, so that the side wall of the jacking block slowly jacks up the jacking column to move upwards along the second through hole groove.
The novel coronavirus multiple PCR rapid detection kit has the beneficial effects that the reagent tube is placed inside the reagent hole, the kit body is made of opaque materials, so that light is prevented from affecting the detection reagent, the reagent tube can be protected by the arranged jacking column, the bottom wall of the reagent tube and the bottom wall of the reagent hole are prevented from generating devices, so that the internal detection reagent is prevented from shaking, then the detection design can be jacked out of the inside of the reagent hole through the arranged driving part, and the detection reagent is convenient to take.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is an exploded view of the novel coronavirus multiplex PCR rapid detection kit of the present invention;
FIG. 2 is a top view of the novel coronavirus multiplex PCR rapid detection kit of the present invention;
FIG. 3 is a schematic diagram of the front sectional view of the multi-PCR rapid detection kit for coronavirus of the present invention;
FIG. 4 is an enlarged view of a portion of FIG. 3;
FIG. 5 is a perspective view of a preferred embodiment of the jacking leg of the present invention;
FIG. 6 is a cross-sectional view of a preferred embodiment of the reagent vessel of the present invention.
In the figure:
1. a kit body; 11. a pull rod groove; 111. a bottom groove; 12. a baffle slot;
2. a reagent well;
3. a protective part; 31. a protective bath; 311. a first through-hole groove; 32. a slide bar; 321. a tension spring; 33. a slider; 331. a first hinge lever; 332. a protective ball; 34. a second through-hole slot;
4. jacking the column; 41. a vertical slot; 42. a spherical block;
5. a drive section; 51. a pull rod body; 52. jacking the block; 53. a second hinge lever; 54. a baffle body;
6. a reagent tube; 61. and (7) positioning a groove.
Detailed Description
The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic views illustrating only the basic structure of the present invention in a schematic manner, and thus show only the constitution related to the present invention.
As shown in fig. 1 to 6, the reagent cartridge body 1 of the present invention, a plurality of reagent wells 2 disposed on the top of the reagent cartridge body 1, a shielding part 3 disposed on the bottom of the reagent wells 2, a driving part 5 mounted on the bottom of the shielding part 3, and reagent tubes 6 mounted inside the reagent wells 2 and 7,7 covering the top of the reagent cartridge body 1 can cover the top wall of the reagent cartridge body 1.
Through placing reagent tube 6 in the inside of reagent hole 2, kit body 1 is light-tight material, make to prevent light influence detect reagent, and can protect reagent tube 6 through the jack-up post 4 that sets up, prevent the diapire of reagent tube 6 and the diapire generating device of reagent hole 2, lead to inside detect reagent to rock, then 5 can be ejecting from the inside of reagent hole 2 with the detection design through the drive division that sets up, convenient taking to detect reagent.
The bottom of the reagent hole 2 is provided with a first through hole groove 311, the bottom of the first through hole groove 311 is provided with a protection groove 31, the inner diameter of the first through hole groove 311 is smaller than that of the reagent hole 2, and the inner diameter of the protection groove 31 is larger than that of the reagent hole 2; the protection part 3 comprises a sliding rod 32 arranged on the inner side wall of the protection groove 31, an expansion spring 321 is sleeved on the sliding rod 32, two groups of sliding blocks 33 are arranged on the surface of the sliding rod 32 in a sliding mode, the top of each sliding block 33 is hinged to a first hinged rod 331, and a protection ball 332 is hinged between every two adjacent groups of first hinged rods 331; the protection ball 332 protrudes out of the first through hole slot 311, wherein when the reagent tube 6 is inserted into the reagent hole 2 and contacts with the protection ball 332, the first through hole slot 311 presses the two sets of sliders 33 to slide along the slide bar 32 to two sides, so as to reduce the shaking of the detection liquid in the reagent hole 2.
Through inserting the inside of detecting design into reagent hole 2, through the first through-hole groove 311 who offers for protection ball 332 can pass first through-hole groove 311, makes protection ball 332 be located inside reagent hole 2, because the gravity of reagent pipe 6 self, can push down the protection ball 332 of bottom, makes slider 33 slide to both sides along slide bar 32 to compress expanding spring 321, make and to play the effect to reagent pipe 6 buffering.
The bottom of reagent pipe 6 is provided with constant head tank 61, and constant head tank 61 is the semicircle setting, and protective ball 332 is the spheroid setting, and protective ball 332 is located the central point of reagent hole 2 bottom and puts, wherein when reagent pipe 6 inserts the inside back in reagent hole 2, constant head tank 61 wraps up protective ball 332 roof.
Through the constant head tank 61 with protection ball 332 roof parcel for can carry on spacingly to the bottom of reagent pipe 6, reagent pipe 6 is all difficult horizontal migration or slope in the inside bottom of inserting reagent hole 2.
The bottom of each of the two side walls of the kit body 1 is provided with a plurality of pull rod grooves 11, the adjacent pull rod grooves 11 are provided with bottom grooves 111, and the driving part 5 comprises a pull rod body (51) arranged in each pull rod groove 11, a jacking block 52 fixed at the top of each pull rod groove 11, and a baffle body 54 hinged between the two adjacent pull rod grooves 11; the elevation section of the jacking block 52 is a right trapezoid, and the top wall of the baffle body 54 is attached to the top wall of the bottom groove 111.
Because after inserting reagent pipe 6 into the inside in reagent hole 2, reagent pipe 6 is whole to be in the inside in reagent hole 2, can be better like this protect reagent hole 2, but inconvenient when taking reagent hole 2, can be ejecting from the bottom in reagent hole 2 with some of reagent pipe 6 through the drive division 5 that sets up, conveniently takes reagent pipe 6.
A plurality of baffle 12 has been seted up at the top of kit body 1, and the internally mounted of baffle 12 has baffle body 54, and jack-up piece 52 keeps apart adjacent two sets of reagent hole 2, and reagent hole 2 is two rows of settings at the top of kit body 1, can keep apart between two rows of reagent hole 2 through the baffle body 54 that sets up, reduces cross infection's risk between the liquid.
The bottom end of the baffle plate 12 is communicated with the top wall of the bottom groove 111; two groups of second hinged rods 53 are hinged to the middle position of the pull rod body 51, the other ends of the two groups of second hinged rods 53 are hinged to the bottom of the baffle body 54, when the pull rod body 51 is completely inserted into the pull rod groove 11, the two groups of second hinged rods 53 are folded in a triangular shape, and the top end of the baffle body 54 is ejected out of the pull rod groove 11.
Through two sets of articulated second articulated rods 53 each other that set up, make and fold, after pull rod body 51 pushed pull rod groove 11's inside completely, second articulated rod 53 becomes triangle-shaped folding, make the top can promote articulated baffle body 54 along baffle 12 rebound, make the top outstanding in the roof of reagent hole 2, baffle body 54 can keep apart between two lines of reagent hole 2, when the roof of reagent box body 1 needs to be cleaned, through the inside of pushing out pull rod groove 11 completely with pull rod body 51, second articulated rod 53 stretches out and draws back to in the baffle 12, make second articulated rod 53 not influence to the cleanness of reagent box body 1 roof.
A second through hole groove 34 is formed in the bottom of the protection groove 31, and the second through hole groove 34 is communicated with the bottom groove 111; jacking post 4 is installed at the top of pull rod body 51, the inside jacking post 4 that the top of jacking post 4 is located second through-hole groove 34 is cylindrical setting, jacking post 4's inside is the cavity setting, perpendicular groove 41 has been seted up to jacking post 4's inside, jacking post 4's bottom is fixed with sphere piece 42, wherein during jacking piece 52 rebound, first hinge bar 331 inserts inside perpendicular groove 41, jacking post 4 passes first through-hole groove 311, jack-up reagent pipe 6, when outside pulling pull rod body 51, jacking piece 52 follows pull rod body 51 and removes, jacking piece 52's hypotenuse position contradicts with sphere piece 42, so that jacking piece 52 lateral wall slowly jacks up jacking post 4 along second through-hole groove 34 rebound.
Through pulling pull rod body 51 to the outside, jack-up piece 52 lateral wall slowly jacks up post 4 along second through-hole groove 34 rebound, and inside first hinge bar 331 inserted perpendicular groove 41 this moment, jack-up post 4 passed first through-hole groove 311, with reagent pipe 6 jack-up for reagent pipe 6's top salient in the roof of reagent hole 2 makes can conveniently take reagent pipe 6, improves detection efficiency.
In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims (6)

1. A novel coronavirus multiple PCR rapid detection kit is characterized by comprising
The kit comprises a kit body (1), a plurality of reagent holes (2) arranged at the top of the kit body (1), a protection part (3) arranged at the bottom of the reagent holes (2), a driving part (5) arranged at the bottom of the protection part (3), and reagent tubes (6) arranged in the reagent holes (2);
a first through hole groove (311) is formed in the bottom of the reagent hole (2), a protection groove (31) is formed in the bottom of the first through hole groove (311), the inner diameter of the first through hole groove (311) is smaller than that of the reagent hole (2), and the inner diameter of the protection groove (31) is larger than that of the reagent hole (2);
the protection part (3) comprises a sliding rod (32) arranged on the inner side wall of the protection groove (31), a telescopic spring (321) is sleeved on the sliding rod (32), two groups of sliding blocks (33) are slidably arranged on the surface of the sliding rod (32), the top of each sliding block (33) is hinged with a first hinge rod (331), and a protection ball (332) is hinged between every two adjacent groups of first hinge rods (331);
the protective ball (332) protrudes from the first through-hole groove (311), wherein
When the reagent tube (6) is inserted into the reagent hole (2) and contacts with the protective ball (332), the first through hole groove (311) presses the two groups of sliding blocks (33) downwards to slide towards two sides along the sliding rod (32) so as to reduce the shaking of the detection liquid in the reagent hole (2);
the bottom of reagent pipe (6) is provided with constant head tank (61), constant head tank (61) are semi-circular setting, protection ball (332) are the spheroid setting, protection ball (332) are located the central point of reagent hole (2) bottom puts, wherein
When the reagent tube (6) is inserted into the reagent hole (2), the positioning groove (61) wraps the top wall of the protective ball (332);
the bottom of two side walls of the kit body (1) is provided with a plurality of pull rod grooves (11), and a bottom groove (111) is arranged adjacent to each pull rod groove (11);
the driving part (5) comprises a pull rod body (51) arranged in the pull rod groove (11), a jacking block (52) fixed at the top of the pull rod groove (11) and a baffle body (54) hinged between two adjacent groups of pull rod grooves (11);
the front view section of the jacking block (52) is in a right trapezoid shape, and the top wall of the baffle body (54) is attached to the top wall of the bottom groove (111).
2. The novel rapid multiplex PCR detection kit for coronavirus according to claim 1,
the reagent kit comprises a reagent kit body (1) and is characterized in that a plurality of baffle plates (12) are arranged at the top of the reagent kit body (1), baffle plate bodies (54) are arranged inside the baffle plates (12), and two adjacent groups of reagent holes (2) are isolated by jacking blocks (52).
3. The novel rapid multiplex PCR detection kit for coronavirus according to claim 2,
the bottom end of the baffle (12) is communicated with the top wall of the bottom groove (111);
the middle position of the pull rod body (51) is hinged with two groups of second hinged rods (53), the other ends of the two groups of second hinged rods (53) are hinged at the bottom of the baffle body (54), wherein
When the pull rod body (51) is completely inserted into the pull rod groove (11), the two groups of second hinge rods (53) are folded in a triangular shape, and the top end of the baffle body (54) is ejected out of the pull rod groove (11).
4. The novel rapid multiplex PCR detection kit for coronavirus according to claim 3,
a second through hole groove (34) is formed in the bottom of the protection groove (31), and the second through hole groove (34) is communicated with the bottom groove (111);
and a jacking column (4) is installed at the top of the pull rod body (51), and the top end of the jacking column (4) is positioned in the second through hole groove (34).
5. The novel rapid multiplex PCR assay kit for coronavirus according to claim 4,
the jacking column (4) is cylindrical, the inner part of the jacking column (4) is hollow, a vertical groove (41) is formed in the inner part of the jacking column (4), a spherical block (42) is fixed at the bottom of the jacking column (4), and the spherical block (42) is fixed at the bottom of the jacking column (4)
When the jacking block (52) moves upwards, the first hinge rod (331) is inserted into the vertical groove (41), and the jacking column (4) penetrates through the first through hole groove (311) to jack up the reagent tube (6).
6. The novel rapid multiplex PCR assay kit for coronavirus according to claim 5,
when the pull rod body (51) is pulled outwards, the jacking block (52) moves along with the pull rod body (51), and the inclined edge position of the jacking block (52) is abutted against the spherical block (42), so that the side wall of the jacking block (52) slowly jacks up the jacking column (4) to move upwards along the second through hole groove (34).
CN202111210449.7A 2021-10-18 2021-10-18 Novel coronavirus multiplex PCR (polymerase chain reaction) rapid detection kit Active CN113753381B (en)

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