CN113736269A - Collagen composite material and preparation method and application thereof - Google Patents
Collagen composite material and preparation method and application thereof Download PDFInfo
- Publication number
- CN113736269A CN113736269A CN202111124012.1A CN202111124012A CN113736269A CN 113736269 A CN113736269 A CN 113736269A CN 202111124012 A CN202111124012 A CN 202111124012A CN 113736269 A CN113736269 A CN 113736269A
- Authority
- CN
- China
- Prior art keywords
- collagen
- high molecular
- molecular polymer
- cellulose
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/48—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with macromolecular fillers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2471/00—Characterised by the use of polyethers obtained by reactions forming an ether link in the main chain; Derivatives of such polymers
- C08J2471/02—Polyalkylene oxides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Materials Engineering (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Manufacturing & Machinery (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Composite Materials (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to the technical field of biological materials, and particularly relates to a collagen composite material and a preparation method and application thereof. The collagen composite material comprises a high molecular polymer and collagen dispersed in the high molecular polymer, wherein the weight ratio of the high molecular polymer to the collagen is 1: (0.01-20). The collagen composite material can effectively reduce the degradation efficiency of collagen and has good mechanical property.
Description
Technical Field
The invention relates to the technical field of biological materials, and particularly relates to a collagen composite material and a preparation method and application thereof.
Background
Collagen is the most abundant protein in the human body and accounts for about 25% -35% of the total protein in the body. Because of its excellent low immunogenicity, it is often used as a material for preparing tissue engineering scaffolds for the repair of damaged tissues. However, the collagen matrix has the disadvantages of fast degradation rate and incapability of maintaining structural stability after being implanted into a human body. For this reason, chemical cross-linking agents are often used to modify the collagen matrix to prolong its degradation time in vivo. Although the chemical cross-linking agent can effectively regulate the degradation time of the collagen matrix, the chemical cross-linking agent has cytotoxicity and can affect the biocompatibility of the collagen matrix, so that the dosage of the chemical cross-linking agent and the regulation degree of the degradation rate have certain limits.
Therefore, it is very important to research a collagen material which is more slowly degraded and safer for human body.
Disclosure of Invention
The present invention aims to overcome the above problems in the prior art and provide a collagen composite material, and a preparation method and application thereof. The collagen composite material can effectively reduce the degradation efficiency of collagen and has good mechanical property.
In order to achieve the above object, a first aspect of the present invention provides a collagen composite material including a high molecular polymer and collagen dispersed therein, wherein a weight ratio of the high molecular polymer to the collagen is 1: (0.01-20).
The inventor of the present invention has found that the degradation rate of collagen can be effectively adjusted by combining a water-soluble polymer safe to the human body with collagen, so that the degradation efficiency of collagen can be significantly reduced.
The above proportion can well achieve the purpose of the present invention, and in order to further achieve a balance between a lower degradation efficiency of collagen and no influence on the beneficial functions of collagen on the human body, preferably, the weight ratio of the high molecular polymer to the collagen is 1: (1-10), more preferably 1: (2-6).
The high molecular polymer may be selected from various water-soluble high molecular polymers safe to the human body.
Preferably, the high molecular polymer has a weight average molecular weight of 10kDa to 500kDa, preferably 100kDa to 300 kDa.
Preferably, the high molecular polymer is selected from one or more of mixtures and/or copolymers of polyethyleneimine, polyvinylpyrrolidone, polyethylene oxide, polyacrylamide, cellulose ethers, cellulose esters, cellulose amides, polyvinyl acetate, polyvinyl alcohol, polyamides, gelatin, methyl cellulose, carboxymethyl cellulose and salts thereof, dextrin, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, maltodextrin, starch, modified starch, guar gum, gum arabic, xanthan gum, chitosan, carrageenan and polyacrylates and salts thereof.
According to a preferred embodiment, the high molecular polymer comprises a combination of two or three of the components a, B and C, each component in the combination being present in an amount of no less than 5% by weight (preferably 10% by weight) based on the total weight of the high molecular polymer;
wherein the A component is selected from one or more of methyl cellulose, carboxymethyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, cellulose ether, cellulose ester and cellulose amide, and the B component is selected from one or more of polyethyleneimine, polyvinylpyrrolidone, polyethylene oxide, polyacrylamide, polyvinyl acetate, polyvinyl alcohol, polyamide and polyacrylate; the C component is selected from one or more of gelatin, chitosan, dextrin, maltodextrin, starch, modified starch, guar gum, gum arabic, xanthan gum and carrageenan.
Preferably, the a component is selected from one or more of carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose and cellulose amide.
Preferably, the B component is selected from one or more of polyvinylpyrrolidone, polyethylene oxide, polyacrylamide and polyvinyl acetate.
Preferably, the C component is selected from one or more of gelatin, carrageenan, guar gum, gum arabic and xanthan gum.
According to a preferred embodiment, the high molecular polymer is a mixture of the component A, the component B and the component C in a weight ratio of 1: (1.2-2.5): (0.4-0.8), more preferably 1: (1.5-2): (0.5-0.7).
In the present invention, the term "collagen" has a meaning generally used in the art, and is a three-dimensional helical structure protein, and also includes various derivatives and variants commonly used in the art for repair, regeneration and reconstruction of defective tissues.
The type of the collagen is not particularly limited in the present invention, and may be selected according to specific needs and clinical modes.
Preferably, the collagen composite has a water content of 10 wt% or less, more preferably 5 wt% or less.
The collagen composite material can be made into various forms according to the application requirement, and is preferably made into a membrane material.
The collagen composite material meeting the characteristics can have good application performance. The collagen composite material can be prepared by various methods, and can meet the characteristics.
After intensive research, the inventor of the invention finds that a proper preparation process can further promote the obtained collagen composite material to have better performance.
Thus according to a preferred embodiment, the second aspect of the present invention provides a method of preparing a collagen composite according to the first aspect of the present invention, the method comprising the steps of:
(1) preparing a collagen solution, wherein the collagen solution is acidic;
(2) preparing a high molecular polymer solution, wherein the high molecular polymer solution is acidic;
(3) and freeze-drying the mixed solution of the collagen solution and the high molecular polymer solution.
The inventor of the present invention found that the synergy between collagen and a high molecular polymer in the collagen composite material obtained can be made more effective by dissolving collagen and a high molecular polymer in an acidic environment, respectively.
In step (1), preferably, the acidity of the collagen solution is [ H ]+]The ion concentration is 0.008 to 0.1mol/L, more preferably 0.02 to 0.09mol/L, and still more preferably 0.05 to 0.08 mol/L.
Preferably, the collagen solution has a collagen content of 1-20mg/ml, preferably 5-12 mg/ml.
The collagen solution may be obtained by dissolving collagen in a first acidic solution.
Preferably, the collagen solution is prepared in a manner including: mixing the collagen with the first acidic solution under the condition of water bath at 4-8 ℃ and stirring until the collagen is completely dissolved.
The first acidic solution may be selected from, for example, one or more of acetic acid, citric acid, hydrochloric acid, sulfuric acid, oxalic acid, and phosphoric acid solutions; preferably one or more of acetic acid, citric acid and oxalic acid. .
In the step (2), preferably, the acidity of the high molecular polymer solution is [ H ]+]The ion concentration is 0.008 to 0.1mol/L, more preferably 0.02 to 0.09mol/L, and still more preferably 0.05 to 0.08 mol/L.
Preferably, the content of the high molecular polymer in the high molecular polymer solution is 1-20mg/ml, preferably 5-12 mg/ml.
The high molecular polymer solution may be obtained by dissolving a high molecular polymer in a second acidic solution.
Preferably, the preparation method of the high molecular polymer solution comprises the following steps: mixing the high molecular polymer and the second acidic solution under the condition of water bath at 40-60 ℃ and stirring until the high molecular polymer is completely dissolved.
The second acidic solution may be selected from one or more of acetic acid, citric acid, hydrochloric acid, sulfuric acid, oxalic acid, and phosphoric acid solutions, for example.
In the step (3), preferably, the mixing of the collagen solution and the high molecular polymer solution is performed at 4 to 8 ℃.
In step (3), preferably, before the freeze-drying, the method further comprises: and degassing the mixed solution of the collagen solution and the high molecular polymer solution. The degassing treatment conditions may include: the vacuum degree is below 20Pa, and the time is 20-60 min.
Preferably, the temperature of the freeze-drying is from-10 ℃ to-30 ℃, more preferably from-15 ℃ to-25 ℃.
In order to achieve the preferred range of water content according to the first aspect of the invention, according to a preferred embodiment, the method further comprises step (4): and (4) carrying out high-temperature vacuum dehydration on the material obtained in the step (3).
Preferably, the high-temperature vacuum dehydration conditions include: the temperature is 102-120 ℃, and more preferably 108-115 ℃; the time is 16h-30 h; the degree of vacuum is 20Pa or less.
In a third aspect of the present invention, there is provided a collagen composite obtained by the production method according to the second aspect.
The collagen composite material according to the third aspect of the present invention has the same properties as the collagen composite material according to the first aspect of the present invention, and thus, the details thereof are not repeated.
The collagen composite material of the invention is especially suitable for the repair, regeneration and reconstruction of defective tissues in animal tissue engineering, for example, the collagen composite material is used as a wound repair material or a scaffold material, because of the excellent properties of slow degradation speed, safety to human bodies, good biocompatibility, good mechanical property and the like. Thus, according to a fourth aspect of the present invention, there is provided the use of a collagen composite according to the first and third aspects in the preparation of a material for use in the engineering of repair, regeneration and reconstruction of defective tissue.
The application mode of the collagen composite material in animal tissue engineering can be determined according to specific needs, and the application can be carried out according to the conventional mode in the field, and the detailed description is omitted.
Through the technical scheme, compared with the prior art, the collagen composite material disclosed by the invention at least has the following advantages:
(1) the degradation speed of the collagen is slower and controllable;
(2) the biological compatibility is good, no chemical cross-linking agent is contained, and the safety is higher for human bodies;
(3) the preparation method is simple and quick;
(4) has good mechanical properties.
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
Detailed Description
The present invention will be described in detail below by way of examples. The described embodiments of the invention are only some, but not all embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following examples and comparative examples, the collagen used was the same. The components used are all commercially available analytical grades unless otherwise specified.
Example 1
(1) Preparing collagen solution
Adjusting the temperature of the cooling water bath of the reaction kettle to 5 ℃, adding collagen and 0.08M acetic acid solution, stirring until the collagen and the acetic acid solution are dissolved, and preparing the concentration of the collagen in the solution to be 10 mg/ml.
(2) Preparing high molecular polymer solution
Adding high molecular polymer (polyethylene oxide, molecular weight 220kDa) and 0.08M acetic acid solution into a sealed glass bottle, putting into a water bath at 50 ℃, stirring until the solution is dissolved, and cooling to room temperature to prepare a solution with the final concentration of 10 mg/ml.
(3) Mixed solution
And stirring and mixing the two solutions in a reaction kettle at the temperature of 5 ℃, wherein the mass ratio of the collagen to the high molecular polymer is 4: 1. After which it is degassed under vacuum at below 20Pa for 30 minutes.
(4) Freeze-drying film
And adding the degassed mixed solution into a freeze-drying mold, and freeze-drying in a freeze dryer. The freezing temperature was set at-20 ℃ and maintained for 2 hours. Then vacuum is carried out for 24 hours under the condition that the freeze-drying vacuum degree is less than 10 Pa.
(5) Thermal treatment
And (3) placing the composite membrane material prepared in the last step into a vacuum drying oven, and carrying out vacuum heat treatment for 24 hours at 110 ℃ to further dehydrate the composite membrane material.
The final collagen composite product was obtained and the water content was measured to be 0.5% by weight.
Example 2
(1) Preparing collagen solution
And adjusting the temperature of the cooling water bath of the reaction kettle to 4 ℃, adding collagen and 0.1M citric acid solution, and stirring until the collagen and the citric acid solution are dissolved, wherein the final concentration of the prepared solution is 5.5 mg/ml.
(2) Preparing high molecular polymer solution
Adding high molecular polymer (chitosan, molecular weight of 160kDa) and 0.1M citric acid solution into a sealed glass bottle, putting into a water bath at 55 ℃, stirring until the solution is dissolved, and cooling to room temperature to prepare a solution with the final concentration of 5.5 mg/ml.
(3) Mixed solution
And stirring and mixing the two solutions in a reaction kettle at the temperature of 5 ℃, wherein the mass ratio of the collagen to the high molecular polymer is 6: 1. After which vacuum degassing was carried out for 30 minutes.
Degassing, freeze-drying film formation and heat treatment were carried out as in example 1.
The final collagen composite product was obtained and the water content was measured to be 0.6% by weight.
Example 3
(1) Preparing collagen solution
And adjusting the temperature of the cooling water bath of the reaction kettle to 8 ℃, adding the collagen and the 0.12M oxalic acid solution, stirring until the collagen and the oxalic acid solution are dissolved, and preparing the solution with the final concentration of 7.5 mg/ml.
(2) Preparing high molecular polymer solution
Adding high molecular polymer (xanthan gum, molecular weight 160Da) and 0.12M oxalic acid solution into a sealed glass bottle, putting into water bath at 60 ℃, stirring to dissolve, cooling to room temperature, and preparing solution with final concentration of 7.5 mg/ml.
(3) Mixed solution
And stirring and mixing the two solutions in a reaction kettle at the temperature of 5 ℃, wherein the mass ratio of the collagen to the high molecular polymer is 2:1 finally.
Degassing, freeze-drying film formation and heat treatment were carried out as in example 1.
The final collagen composite product was obtained and the water content was measured to be 0.8% by weight.
EXAMPLE 4 group
This set of examples is presented to illustrate the effect of high molecular weight polymers.
This set of examples was conducted as in example 1, except that the composition of the high molecular weight polymer was changed separately while keeping the total content constant. Specifically, the method comprises the following steps:
example 4 a: the high molecular polymer is a combination of two components, specifically hydroxypropyl methylcellulose and polyethyleneimine in a weight ratio of 1: 2, having a weight average molecular weight of 150 kDa;
example 4 b: the high molecular polymer is a combination of two components, specifically polyvinyl acetate and gum arabic in a weight ratio of 1: 0.2, weight average molecular weight is 280 kDa;
example 4 c: the high molecular polymer is a combination of two components, specifically hydroxyethyl cellulose and gelatin in a weight ratio of 1: 0.5, weight average molecular weight 110 kDa;
example 4 d: the high molecular polymer is a combination of three components, specifically carboxymethyl cellulose, polyvinyl acetate and guar gum in a weight ratio of 1: 1.5: 0.7, weight average molecular weight 180 kDa;
example 4 e: the high molecular polymer is a combination of three components, specifically cellulose amide, polyvinylpyrrolidone and carrageenan in a weight ratio of 1: 2: 0.5, weight average molecular weight 210 kDa;
respectively obtaining the final collagen composite material products.
Example 5
The procedure was as in example 1, except that the ratio of the high molecular weight polymer to the collagen was changed while keeping the total content of the two constant. Specifically, the mass ratio of collagen to high-molecular polymer was 10: 1.
Finally obtaining the collagen composite material product.
Comparative example 1
Reference was made to example 1, except that collagen alone was contained and the high molecular weight polymer was not contained. Specifically, the method comprises the following steps:
and adjusting the temperature of the cooling water bath of the reaction kettle to 5 ℃, adding the collagen raw material and 0.08M acetic acid solution, and stirring until the collagen raw material and the acetic acid solution are dissolved, wherein the final concentration of the prepared solution is 5 mg/ml.
Degassing, freeze-drying film formation and heat treatment were carried out as in example 1.
Obtaining the final collagen material product.
Test example
The materials obtained in the examples and comparative examples were subjected to the following tests, respectively:
(1) amount of enzyme residue (%)
Performing enzyme degradation on the obtained material under the same condition, calculating the percentage of the residual amount after degradation to the weight before degradation, and recording the result in table 1, wherein the higher the value is, the more the residual amount is, the less the material is easy to degrade;
(2) mechanical Properties
The resulting material was tested for tensile strength (kPa) and the results are reported in table 1.
TABLE 1
As can be seen from Table 1, the collagen composite material of the present invention has a high residual amount after degradation, can effectively reduce the degradation efficiency of collagen, and has good mechanical properties.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. A collagen composite material, which is characterized by comprising a high molecular polymer and collagen dispersed therein, wherein the weight ratio of the high molecular polymer to the collagen is 1: (0.01-20).
2. The collagen composite according to claim 1, wherein the weight ratio of the high molecular polymer to the collagen is 1: (1-10), preferably 1: (2-6).
3. The collagen composite according to claim 1 or 2, wherein the high molecular polymer is a water-soluble high molecular polymer selected from one or more of a mixture and/or copolymer of polyethyleneimine, polyvinylpyrrolidone, polyethylene oxide, polyacrylamide, cellulose ether, cellulose ester, cellulose amide, polyvinyl acetate, polyvinyl alcohol, polyamide, gelatin, methyl cellulose, carboxymethyl cellulose and salts thereof, dextrin, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, maltodextrin, starch, modified starch, guar gum, gum arabic, xanthan gum, carrageenan and polyacrylate and salts thereof;
preferably, the high molecular polymer has a weight average molecular weight of 10kDa to 500kDa, more preferably 100kDa to 300 kDa.
4. The collagen composite according to claim 1 or 2, wherein the high molecular polymer comprises a combination of two or three of the components A, B and C, wherein the content of each component in the combination is not less than 5 wt% of the total weight of the high molecular polymer; wherein the A component is selected from one or more of methyl cellulose, carboxymethyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, cellulose ether, cellulose ester and cellulose amide, and the B component is selected from one or more of polyethyleneimine, polyvinylpyrrolidone, polyethylene oxide, polyacrylamide, polyvinyl acetate, polyvinyl alcohol, polyamide and polyacrylate; the C component is selected from one or more of gelatin, chitosan, dextrin, maltodextrin, starch, modified starch, guar gum, gum arabic, xanthan gum and carrageenan;
preferably, the a component is selected from one or more of carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose and cellulose amide; the component B is selected from one or more of polyvinylpyrrolidone, polyethylene oxide, polyacrylamide and polyvinyl acetate; the C component is selected from one or more of gelatin, carrageenan, guar gum, gum arabic and xanthan gum.
5. A method for preparing a collagen composite according to any one of claims 1 to 4, comprising the steps of:
(1) preparing a collagen solution, wherein the collagen solution is acidic;
(2) preparing a high molecular polymer solution, wherein the high molecular polymer solution is acidic;
(3) and freeze-drying the mixed solution of the collagen solution and the high molecular polymer solution.
6. The method according to claim 5, wherein, in step (1), the acidity of the collagen solution is [ H ]+]The ion concentration is 0.008-0.1mol/L, preferably 0.05-0.08 mol/L;
preferably, the collagen solution contains collagen in an amount of 1-20 mg/ml.
7. According to the rightThe method of claim 5, wherein, in the step (2), the acidity of the high molecular polymer solution is [ H ]+]The ion concentration is 0.008-0.1mol/L, preferably 0.05-0.08 mol/L;
preferably, the content of the high molecular polymer in the high molecular polymer solution is 1-20 mg/ml.
8. The method according to any one of claims 5 to 7, wherein in step (3), before the freeze-drying, further comprising: performing degassing treatment on the mixed solution of the collagen solution and the high molecular polymer solution, wherein the degassing treatment conditions comprise: the vacuum degree is below 20Pa, and the time is 20-60 min;
preferably, the method further comprises step (4): and (4) carrying out high-temperature vacuum dehydration on the material obtained in the step (3).
9. The collagen composite material obtained by the production method according to any one of claims 5 to 8.
10. Use of a collagen composite according to any one of claims 1 to 4 and 9 for the preparation of a material for use in the engineering of defect tissue repair, regeneration and reconstruction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111124012.1A CN113736269A (en) | 2021-09-24 | 2021-09-24 | Collagen composite material and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111124012.1A CN113736269A (en) | 2021-09-24 | 2021-09-24 | Collagen composite material and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113736269A true CN113736269A (en) | 2021-12-03 |
Family
ID=78740788
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111124012.1A Pending CN113736269A (en) | 2021-09-24 | 2021-09-24 | Collagen composite material and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113736269A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114717057A (en) * | 2022-03-18 | 2022-07-08 | 九江德福科技股份有限公司 | Cleaning solution and cleaning method for cleaning collagen adding system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2159259A1 (en) * | 2008-08-28 | 2010-03-03 | Fujifilm Corporation | A structure comprising chitosan and collagen |
| CN111001039A (en) * | 2019-12-16 | 2020-04-14 | 天新福（北京）医疗器材股份有限公司 | Nerve injury repair material and preparation method and application thereof |
| CN112842931A (en) * | 2021-02-04 | 2021-05-28 | 甘肃天际生物科技有限公司 | Animal collagen freeze-dried ball, cosmetic set and preparation process |
-
2021
- 2021-09-24 CN CN202111124012.1A patent/CN113736269A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2159259A1 (en) * | 2008-08-28 | 2010-03-03 | Fujifilm Corporation | A structure comprising chitosan and collagen |
| CN111001039A (en) * | 2019-12-16 | 2020-04-14 | 天新福（北京）医疗器材股份有限公司 | Nerve injury repair material and preparation method and application thereof |
| CN112842931A (en) * | 2021-02-04 | 2021-05-28 | 甘肃天际生物科技有限公司 | Animal collagen freeze-dried ball, cosmetic set and preparation process |
Non-Patent Citations (6)
| Title |
|---|
| 伍津津等主编: "《皮肤组织工程学》", vol. 1, 人民军医出版社, pages: 58 - 63 * |
| 张晓莉等: ""胶原蛋白/聚乙烯醇混合液的流变特性及复合膜性能的研究"", 《中原工学院学报》 * |
| 张晓莉等: ""胶原蛋白/聚乙烯醇混合液的流变特性及复合膜性能的研究"", 《中原工学院学报》, vol. 25, no. 6, 31 December 2014 (2014-12-31), pages 32 - 35 * |
| 李晓静: ""壳聚糖-胶原膜引导种植体颊侧裂隙状缺损骨再生的实验研究及纳米纤维膜的初步研究"", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
| 李晓静: ""壳聚糖-胶原膜引导种植体颊侧裂隙状缺损骨再生的实验研究及纳米纤维膜的初步研究"", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》, no. 1, 15 January 2015 (2015-01-15), pages 074 - 121 * |
| 王碧等: ""胶原蛋白—壳聚糖—聚乙烯吡咯烷酮共混膜的结构及性能"", 《中国皮革》, vol. 36, no. 3, 28 February 2007 (2007-02-28), pages 22 - 24 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114717057A (en) * | 2022-03-18 | 2022-07-08 | 九江德福科技股份有限公司 | Cleaning solution and cleaning method for cleaning collagen adding system |
| CN114717057B (en) * | 2022-03-18 | 2023-12-12 | 九江德福科技股份有限公司 | Cleaning liquid and cleaning method for cleaning collagen adding system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103467760B (en) | A kind of method preparing high-strength chitosan/cellulose composite hydrogel film | |
| CN110522948B (en) | Injectable hydrogel and preparation method and application thereof | |
| CN113845668B (en) | Polysaccharide composite hydrogel and preparation method and application thereof | |
| US20130154139A1 (en) | Process for producing cellulose shaped articles | |
| EP2956184B9 (en) | Silk based porous scaffold and a process for the preparation thereof | |
| CN114470330B (en) | Recombinant collagen gel particles for tissue filling and preparation method thereof | |
| JP2023064714A (en) | Hydrophilic injection-type skin filling composition, and preparation method and application thereof | |
| CN107118361B (en) | Silk fibroin/carboxymethyl chitosan composite gel and preparation method thereof | |
| CN113736269A (en) | Collagen composite material and preparation method and application thereof | |
| CN111732741A (en) | Method for crosslinking hyaluronic acid and polylysine, composite crosslinked product obtained by method and application of composite crosslinked product | |
| Wang et al. | Fabrication, characterization and potential application of biodegradable polydopamine-modified scaffolds based on natural macromolecules | |
| CN108478874B (en) | Preparation method of hydroxyethyl chitosan nano composite bone scaffold material | |
| CN116144082A (en) | Sodium hyaluronate and chitosan composite gel and preparation method and application thereof | |
| CN104448843B (en) | A kind of method that polycaprolactone improves poly-peptide film hydrophily and compliance with polyacrylic acid | |
| CN110540661A (en) | Composite hydrogel of silk fibroin and polyvinyl alcohol, and preparation method and application thereof | |
| RU2408746C1 (en) | Method of producing chitosan-containing threads | |
| CN103480038B (en) | Preparation method for chitosan/hydroxyapatite composite hydrogel | |
| JP2000516278A (en) | Method for producing cellulosic molded article | |
| Wei et al. | Gelatin/carboxymethyl chitosan/aloe juice hydrogels with skin-like endurance and quick recovery: Preparation, characterization, and properties | |
| CN105670307A (en) | Method for improving water resistance and flexibility of polyvinyl alcohol membrane through polycaprolactone and poly(trimethylene carbonate)-polyvinylpyrrolidone | |
| CN114470307B (en) | Degradable hemostatic plugging adhesive and preparation method thereof | |
| CN116421771B (en) | Novel porous starch hemostatic powder and preparation method and application thereof | |
| You et al. | All-aqueous-processed Silk Fibroin/Chondroitin Sulfate Scaffolds | |
| CN112876696B (en) | Biomedical polyglyceryl hydrogel and preparation method thereof | |
| CN117599247B (en) | Silk fibroin microcarrier skin filling preparation, and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |